GCF_003977665.1 - 20 region(s)

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Location: 377,967 - 388,812 nt. (total: 10,846 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: sulfotransferase

EKH80_RS01865: glutathione S-transferase

smcogs: SMCOG1193: glutathione S-transferase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002133.3	0.36	ribosomal	group 1 methanobactin	Methylosinus sp. LW3
BGC0000404.4	0.35	NRPS	penicillin	Penicillium chrysogenum
BGC0001484.3	0.31	other (other)	2'-chloropentostatin, 2'-amino-2'-deoxyadenosine	Actinomadura sp. ATCC 39365
BGC0001285.5	0.31	other (fatty acid)	pseudopyronine A, pseudopyronine B	Pseudomonas putida
BGC0001369.3	0.23	PKS	metatricycloene	uncultured bacterium

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002133.3	0.36	ribosomal	group 1 methanobactin	Methylosinus sp. LW3
BGC0000404.4	0.35	NRPS	penicillin	Penicillium chrysogenum
BGC0001484.3	0.31	other (other)	2'-chloropentostatin, 2'-amino-2'-deoxyadenosine	Actinomadura sp. ATCC 39365
BGC0001285.5	0.31	other (fatty acid)	pseudopyronine A, pseudopyronine B	Pseudomonas putida
BGC0001369.3	0.23	PKS	metatricycloene	uncultured bacterium

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.71 out of 26.33

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.59 out of 24.78

Confidence: medium

TFBS Finder

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
LexA variant 2	Repressor of DNA damage response

NZ_RYYV01000010.1 - Region 1 - NRPS

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Location: 78,376 - 122,374 nt. (total: 43,999 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acetyltransferase aminotransferase glycosyltransferase transferase

EKH80_RS14305: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

EKH80_RS14345: aminotransferase class V

smcogs: SMCOG1139: aminotransferase class V

EKH80_RS14385: glycosyltransferase

smcogs: SMCOG1102: glycosyltransferase

EKH80_RS14415: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

EKH80_RS14445: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

Hydrolases: deacetylase

EKH80_RS14460: acetylornithine deacetylase

smcogs: SMCOG1247: acetylornithine deacetylase

Ligases: ligase

EKH80_RS14380: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002075.3	0.74	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001833.4	0.67	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001132.4	0.67	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001758.4	0.67	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.66	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.65	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.64	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.63	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002075.3	0.74	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001833.4	0.67	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001132.4	0.67	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001758.4	0.67	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.66	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.65	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.64	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.63	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 20.76 out of 21.84

Confidence: strong

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 19.02 out of 21.84

Confidence: medium

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 16.31 out of 18.57

Confidence: medium

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 22.87 out of 26.33

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.94 out of 20.97

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 22.95 out of 27.78

Confidence: medium

Additional weak hits (6):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.48 out of 27.78

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.64 out of 20.01

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.46 out of 20.97

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 19.35 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.22 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.42 out of 26.06

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 78375 - 122374:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

EKH80_RS14380: X

AMP-binding (459..851): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LGMAFDIALLELWALVGGETQLYGPTETTVLSTS
Nearest Stachelhaus code(s):: Gly DILQLGLVWK (74% 8Å match)
Stachelhaus code match:: 80% (moderate)

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
CcpA	Catabolite control protein A
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
MexT	Global virulence regulator
ToxT	Regulator of MSHA biosynthesis

NZ_RYYV01000013.1 - Region 1 - terpene

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Location: 40,889 - 61,755 nt. (total: 20,867 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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other genes

resistance

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Terpene

TFBS Finder

Gene/CDS overview

Filter:

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: carbamoyltransferase methyltransferase

EKH80_RS16305: methyltransferase

smcogs: SMCOG1089: methyltransferase

EKH80_RS16375: ornithine carbamoyltransferase

smcogs: SMCOG1114: ornithine carbamoyltransferase

Hydrolases: hydrolase

EKH80_RS16310: HAD-superfamily hydrolase, subfamily IA, variant

smcogs: SMCOG1115: HAD-superfamily hydrolase, subfamily IA, variant

Lyases: cyclase

EKH80_RS16280: diguanylate cyclase

smcogs: SMCOG1025: diguanylate cyclase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001444.5	0.35	other (shikimate-derived)	caboxamycin	Streptomyces sp. NTK 937
BGC0001985.4	0.35	other (other)	Pyrazofurin	Streptomyces candidus
BGC0002657.2	0.32	NRPS, PKS	deoxyhangtaimycin	Streptomyces spectabilis
BGC0000874.5	0.32	other (other)	blasticidin S	Streptomyces griseochromogenes
BGC0002443.3	0.32	PKS	mycemycin C, mycemycin A, mycemycin B	Streptomyces olivaceus
BGC0002571.2	0.30	NRPS	pyoverdine DC3000	Pseudomonas syringae pv. tomato str. DC3000
BGC0002661.2	0.29	NRPS	tasikamide A, tasikamide B, tasikamide C	Streptomyces tasikensis
BGC0002491.3	0.28	NRPS	myxochelin B, myxochelin A, pseudochelin A	Pseudoalteromonas piscicida
BGC0000981.3	0.28	NRPS, PKS	cylindrospermopsin	Aphanizomenon sp. 10E6
BGC0002713.2	0.28	NRPS	mevalagmapeptide A, mevalagmapeptide B, mevalagmapeptide C, mevalagmapeptide D	Photorhabdus laumondii subsp. laumondii

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001444.5	0.35	other (shikimate-derived)	caboxamycin	Streptomyces sp. NTK 937
BGC0001985.4	0.35	other (other)	Pyrazofurin	Streptomyces candidus
BGC0002657.2	0.32	NRPS, PKS	deoxyhangtaimycin	Streptomyces spectabilis
BGC0000874.5	0.32	other (other)	blasticidin S	Streptomyces griseochromogenes
BGC0002443.3	0.32	PKS	mycemycin C, mycemycin A, mycemycin B	Streptomyces olivaceus
BGC0002571.2	0.30	NRPS	pyoverdine DC3000	Pseudomonas syringae pv. tomato str. DC3000
BGC0002661.2	0.29	NRPS	tasikamide A, tasikamide B, tasikamide C	Streptomyces tasikensis
BGC0002491.3	0.28	NRPS	myxochelin B, myxochelin A, pseudochelin A	Pseudoalteromonas piscicida
BGC0000981.3	0.28	NRPS, PKS	cylindrospermopsin	Aphanizomenon sp. 10E6
BGC0002713.2	0.28	NRPS	mevalagmapeptide A, mevalagmapeptide B, mevalagmapeptide C, mevalagmapeptide D	Photorhabdus laumondii subsp. laumondii

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
triterpene		squalene	C30	acyclic	none
triterpene	C30 carotenoid	diapophytoene	C30	acyclic	none
triterpene		PSPP	C30	acyclic	PP
tetraterpene	C40 carotenoid	phytoene	C40	acyclic	none
triterpene		hydroxysqualene	C30	acyclic	OH

Genes with terpene domains:

EKH80_RS16315: PT_phytoene_like

Domain 1 (61 - 214): Prenyltransferase; Phytoene synthase like

Subtype: unknown

Reactions:: FPP → squalene; FPP → diapophytoene; FPP → PSPP; GGPP → phytoene; PSPP → hydroxysqualene

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.18 out of 20.47

Confidence: medium

Additional weak hits (7):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.96 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.78 out of 27.78

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.27 out of 18.57

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.74 out of 30.22

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.44 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.43 out of 28.19

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 22.12 out of 30.98

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
FPP	Farnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
PP	Diphosphate
PSPP	Presqualene diphosphate

TFBS definitions

Regulator	Description
VqsM	Regulator of quorum-sensing signalling systems

NZ_RYYV01000013.1 - Region 2 - arylpolyene

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Location: 77,803 - 125,463 nt. (total: 47,661 nt) Show pHMM detection rules used

arylpolyene: (APE_KS1 or APE_KS2)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

EKH80_RS16530: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

EKH80_RS16540: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acetyltransferase acyltransferase carboxylase methyltransferase transferase

EKH80_RS16475: acetyl-CoA carboxylase biotin carboxylase

smcogs: SMCOG1090: acetyl-CoA carboxylase biotin carboxylase

EKH80_RS16510: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

EKH80_RS16520: acetyl-CoA acetyltransferase

smcogs: SMCOG1124: acetyl-CoA acetyltransferase

EKH80_RS16555: methyltransferase

smcogs: SMCOG1089: methyltransferase

EKH80_RS16585: acyltransferase

smcogs: SMCOG1141: acyltransferase

Hydrolases: protease

EKH80_RS16525: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Lyases: hydratase

EKH80_RS16485: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Ligases: synthase

EKH80_RS16470: 2-isopropylmalate synthase

smcogs: SMCOG1271: 2-isopropylmalate synthase

EKH80_RS16550: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

EKH80_RS16595: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

EKH80_RS16610: polyprenol-monophosphomannose synthase ppm1

smcogs: SMCOG1123: polyprenol-monophosphomannose synthase ppm1

Translocases: transport

EKH80_RS16600: MMPL domain-containing transport protein

smcogs: SMCOG1035: MMPL domain-containing transport protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001160.5	0.60	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.59	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000866.5	0.55	other (other)	polyhydroxyalkanoate	Burkholderia sp. DSM 9242
BGC0000867.3	0.52	other (other)	polyhydroxyalkanoic acids	Ectothiorhodospira shaposhnikovii
BGC0000234.5	0.50	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0001987.2	0.48	PKS	koreenceine A, koreenceine B, koreenceine C, koreenceine D	Pseudomonas koreensis
BGC0001197.5	0.47	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0002477.3	0.47	PKS	thioangucycline A, thioangucycline B	Streptomyces sp. CB00072
BGC0000231.5	0.47	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001917.4	0.47	PKS	5aTHQ-9i, 5aTHQ-9n, 5aTHQ-10a, STAMs-9i, STAMs-9n	Streptomyces nigrescens

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001160.5	0.60	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.59	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000866.5	0.55	other (other)	polyhydroxyalkanoate	Burkholderia sp. DSM 9242
BGC0000867.3	0.52	other (other)	polyhydroxyalkanoic acids	Ectothiorhodospira shaposhnikovii
BGC0000234.5	0.50	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0001987.2	0.48	PKS	koreenceine A, koreenceine B, koreenceine C, koreenceine D	Pseudomonas koreensis
BGC0001197.5	0.47	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0002477.3	0.47	PKS	thioangucycline A, thioangucycline B	Streptomyces sp. CB00072
BGC0000231.5	0.47	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001917.4	0.47	PKS	5aTHQ-9i, 5aTHQ-9n, 5aTHQ-10a, STAMs-9i, STAMs-9n	Streptomyces nigrescens

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 24.45 out of 26.06

Confidence: strong

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.55 out of 18.46

Confidence: strong

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 21.45 out of 25.31

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.80 out of 20.97

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.80 out of 20.47

Confidence: medium

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 18.02 out of 20.01

Confidence: medium

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.81 out of 18.57

Confidence: medium

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 24.14 out of 26.12

Confidence: medium

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.81 out of 18.57

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.79 out of 20.97

Confidence: medium

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 24.24 out of 30.25

Confidence: medium

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.58 out of 21.84

Confidence: medium

Additional weak hits (17):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.85 out of 26.85

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 20.73 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 23.93 out of 32.74

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.82 out of 20.01

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.78 out of 26.06

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 18.39 out of 28.14

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 18.32 out of 26.12

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.65 out of 26.06

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.59 out of 18.57

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.31 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.65 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.92 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.97 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 18.94 out of 26.06

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.43 out of 28.14

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.85 out of 20.01

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.56 out of 39.62

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

TFBS Finder

TFBS definitions

Regulator	Description
ArgR	Regulator of arginine biosynthesis genes
CatR	H2O2-responsive repressor
CcpA	Catabolite control protein A
ColR	Phenol-responsive regulator
LuxO	Repressor of luminescence
MexT	Global virulence regulator
MogR	Repressor of flagellar motility genes
RpoN	Regulator of nitrogen assimilation and virulence
ToxT	Regulator of MSHA biosynthesis
VqsM	Regulator of quorum-sensing signalling systems

NZ_RYYV01000019.1 - Region 1 - indole

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Location: 38,887 - 60,878 nt. (total: 21,992 nt) Show pHMM detection rules used

indole: (indsynth or dmat or indole_PTase)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

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TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

EKH80_RS19290: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Lyases: cyclase

EKH80_RS19240: diguanylate cyclase

smcogs: SMCOG1025: diguanylate cyclase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001197.5	0.41	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0001810.3	0.39	PKS	trehangelin	Polymorphospora rubra
BGC0000204.5	0.38	PKS	benastatin A, benastatin B, BE-43767, TAN1532B	Streptomyces sp. A2991200
BGC0000218.5	0.38	PKS	doxorubicin	Streptomyces peucetius
BGC0002138.2	0.38	PKS	fuelimycin A, fuelimicin B, fuelimicin C	Streptomyces albireticuli NRRL B-1670
BGC0001782.3	0.38	other (other)	spiroindimicin A, spiroindimicin B, spiroindimicin C, spiroindimicin D, indimicin A, indimicin B, indimicin C, indimicin D, indimicin E, lynamicin A, lynamicin D, lynamicin F, lynamicin G	Streptomyces sp. SCSIO 03032
BGC0000384.6	0.36	NRPS	lyngbyatoxin	Lyngbya majuscula
BGC0002746.3	0.36	ribosomal	dendrothelin A	Dendrothele bispora CBS 962.96
BGC0001987.2	0.35	PKS	koreenceine A, koreenceine B, koreenceine C, koreenceine D	Pseudomonas koreensis
BGC0001768.4	0.35	NRPS	sansalvamide	[Nectria] haematococca mpVI 77-13-4

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001197.5	0.41	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0001810.3	0.39	PKS	trehangelin	Polymorphospora rubra
BGC0000204.5	0.38	PKS	benastatin A, benastatin B, BE-43767, TAN1532B	Streptomyces sp. A2991200
BGC0000218.5	0.38	PKS	doxorubicin	Streptomyces peucetius
BGC0002138.2	0.38	PKS	fuelimycin A, fuelimicin B, fuelimicin C	Streptomyces albireticuli NRRL B-1670
BGC0001782.3	0.38	other (other)	spiroindimicin A, spiroindimicin B, spiroindimicin C, spiroindimicin D, indimicin A, indimicin B, indimicin C, indimicin D, indimicin E, lynamicin A, lynamicin D, lynamicin F, lynamicin G	Streptomyces sp. SCSIO 03032
BGC0000384.6	0.36	NRPS	lyngbyatoxin	Lyngbya majuscula
BGC0002746.3	0.36	ribosomal	dendrothelin A	Dendrothele bispora CBS 962.96
BGC0001987.2	0.35	PKS	koreenceine A, koreenceine B, koreenceine C, koreenceine D	Pseudomonas koreensis
BGC0001768.4	0.35	NRPS	sansalvamide	[Nectria] haematococca mpVI 77-13-4

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.59 out of 23.05

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 20.15 out of 23.79

Confidence: medium

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 23.74 out of 29.72

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 22.53 out of 28.19

Confidence: medium

Additional weak hits (9):

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 27.09 out of 39.62

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 19.47 out of 30.25

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 20.96 out of 36.54

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.62 out of 29.38

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 19.40 out of 26.12

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.70 out of 26.33

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 20.66 out of 30.25

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.36 out of 29.65

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.13 out of 25.75

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
IolR	Regulator of the inositol catabolism
NtrC	Nitrogen regulatory protein C
ZuR variant 1	Zinc-responsive repressor

NZ_RYYV01000002.1 - Region 1 - NRPS

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Location: 15,441 - 62,595 nt. (total: 47,155 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

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TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

EKH80_RS02930: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

EKH80_RS02960: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

EKH80_RS02980: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acyltransferase aminotransferase methyltransferase

EKH80_RS02915: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

EKH80_RS02935: methyltransferase

smcogs: SMCOG1089: methyltransferase

EKH80_RS02945: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

Ligases: ligase synthase

EKH80_RS02955: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

EKH80_RS02990: Pseudouridine synthase

smcogs: SMCOG1154: Pseudouridine synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002075.3	0.73	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001833.4	0.71	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.71	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.70	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001758.4	0.70	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.69	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.69	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000342.4	0.68	NRPS	enniatin	Fusarium equiseti
BGC0001135.5	0.68	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002075.3	0.73	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001833.4	0.71	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.71	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.70	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001758.4	0.70	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.69	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.69	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000342.4	0.68	NRPS	enniatin	Fusarium equiseti
BGC0001135.5	0.68	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.55 out of 18.46

Confidence: strong

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 16.82 out of 18.46

Confidence: medium

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 16.08 out of 18.57

Confidence: medium

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.54 out of 26.33

Confidence: medium

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 20.49 out of 23.43

Confidence: medium

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 16.82 out of 18.46

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

Additional weak hits (19):

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 23.30 out of 36.54

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 20.11 out of 33.73

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 21.21 out of 36.54

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.75 out of 26.06

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.11 out of 18.57

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.90 out of 30.22

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.79 out of 31.73

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.64 out of 29.65

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.63 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.69 out of 26.06

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 18.62 out of 29.25

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.54 out of 26.06

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.25 out of 28.14

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.30 out of 21.84

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.50 out of 39.62

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.69 out of 28.19

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.54 out of 35.63

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.93 out of 28.19

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.97 out of 35.63

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 15440 - 62595:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - Cys)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

EKH80_RS02945: X - Cys

Search NORINE for peptide: strict or relaxed

AMP-binding (474..872): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: LWQAFDVSFQEAFLITAGEYNQYGPTETHLASEL
Nearest Stachelhaus code(s):: 4Me-Pro DVQFIAHLAK (74% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (1528..1934): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: LWITFDLSVYDGDSFFSGDIGLGGATEASVWSNY
Nearest Stachelhaus code(s):: Cys DLFDFSLVWK (85% 8Å match)
Stachelhaus code match:: 90% (moderate)

EKH80_RS02955: X

AMP-binding (32..446): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: IAVPMIGFLYNVYAVTGSAQALWGMTEIGGATFT
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
AbrC3	Antibiotic production activator
BldD	Development and antibiotic global regulator
RpoN	Regulator of nitrogen assimilation and virulence
ToxT	Regulator of MSHA biosynthesis

NZ_RYYV01000002.1 - Region 2 - lanthipeptide-class-ii

Download region SVG

Location: 160,886 - 183,753 nt. (total: 22,868 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

EKH80_RS03415: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

EKH80_RS03425: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Hydrolases: dehalogenase

EKH80_RS03480: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

Lyases: cyclase hydratase

EKH80_RS03450: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

EKH80_RS03470: Nitrilase/cyanide hydratase and apolipoprotein

smcogs: SMCOG1294: Nitrilase/cyanide hydratase and apolipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002276.2	0.55	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001928.2	0.48	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0000554.4	0.48	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0000528.5	0.48	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0001579.4	0.46	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0000894.4	0.45	other (other)	citrinin	Monascus aurantiacus
BGC0000243.4	0.43	PKS	nonactin, monactin, dinactin, trinactin, tetranactin	Streptomyces griseus subsp. griseus
BGC0000503.5	0.43	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0002698.2	0.43	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0001364.3	0.43	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002276.2	0.55	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001928.2	0.48	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0000554.4	0.48	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0000528.5	0.48	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0001579.4	0.46	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0000894.4	0.45	other (other)	citrinin	Monascus aurantiacus
BGC0000243.4	0.43	PKS	nonactin, monactin, dinactin, trinactin, tetranactin	Streptomyces griseus subsp. griseus
BGC0000503.5	0.43	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0002698.2	0.43	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0001364.3	0.43	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for EKH80_RS03450

EKH80_RS03455

Leader:

MDTVLEGEALVAQWRASADVDSPAG

Core:

PLFIGGKYAKADIVNSAESITTNFLCTCGTAIHDNCCN

Core with dehydrations:

PLFIGGKYAKADIVNDhaAEDhaIDhbDhbNFLCysDhbCysGDhbAIHDNCysCysN

antiSMASH-DB 4.0 matches for EKH80_RS03455

100.0%

Lanthipeptides: EKH80_RS03455_lanthipeptide

1

PLFIGGKYAKADIVNSAESITTNFLCTCGTAIHDNCCN

38

PLFIGGKYAKADIVNSAESITTNFLCTCGTAIHDNCCN

1

PLFIGGKYAKADIVNSAESITTNFLCTCGTAIHDNCCN

38

40.0%

NZ_FOZI01000001.1:

Lanthipeptides: BM338_RS00935_lanthipeptide

1

PLFIGGKYAKADIVNSAESITTNFLCTCGTA

31

PLF GG++A+A+I++ A S+TT CGTA

2

PLFTGGQFAEAEIISEASSLTT--FGGCGTA

30

39.6%

NZ_FNOG01000007.1:

Lanthipeptides: BLU84_RS27270_lanthipeptide

1

PLFIGGKYAKADIVNSAESIT--TNFLCTCGTAIHDNCC

37

PLFIGG++A+ADI +E+++ T+ CGT IH CC

13

PLFIGGEFAEADIAGDSEAVSMITSVCTGCGT-IH--CC

48

with 53 more sequence matches

Only the first 10 are displayed here.

39.5%

NZ_CP064030.1:

Lanthipeptides: ISN74_RS08625_lanthipeptide

1

PLFIGGKYAKADIVNSAESITTNFLCT--CGTA----IHDNCC

37

PLF+ G++A++DIV+S +T +C+ CG+A H CC

1

PLFVSGQFAESDIVSSTNVLTLGAVCSGRCGSACSGSAHAECC

43

39.5%

NZ_JADIKF010000040.1:

Lanthipeptides: ISS99_RS17245_lanthipeptide

1

PLFIGGKYAKADIVNSAESITTNFLCT--CGT----AIHDNCC

37

PLF+ G++A++DIV+S +T +C+ CGT + H CC

1

PLFVSGRFAESDIVSSTNIVTAYAVCSGRCGTICSGSSHAECC

43

35.4%

NZ_CP072597.1:

Lanthipeptides: J5226_RS01600_lanthipeptide

1

PLFIGGKYAKADIVNSAESITTN-FLCT-CGT

30

PLFI G++A+ADI E I+ N CT CGT

13

PLFISGEFAEADIAGDNEIISRNTSACTGCGT

44

35.2%

NZ_CP076103.1:

Lanthipeptides: KME82_RS02870_lanthipeptide

1

PLFIGGKYAKADIVNS-AESITTNFLCTCGTAIHDNCC

37

PLFIGG+Y ++DI+ A +IT LCT +I NCC

18

PLFIGGEYVESDIIGEVAPAITGCSLCT--GSIRINCC

53

34.2%

NZ_CP041730.1:

Lanthipeptides: FNU76_RS16940_lanthipeptide

2

LFIGGKYAKADIVNSAESITTNFLCTCGTA

31

L++ G+YA+ADI S +T CGTA

1

LYVSGEYAEADIAMSIRPLTWG---GCGTA

27

34.1%

BGC0000535.4 (ADJ56352.1):

Lanthipeptides: allorf_108254_108448_lanthipeptide

1

PLFIGGKYAKADIVN-SAESITTNFLCTCGTAIHDNCC

37

PLF+ G K SAE I T F T A +CC

7

PLFVDGSTEKKMTTRASAERIDTMFRTTQSCASGCSCC

44

34.0%

NZ_CP040709.1:

Lanthipeptides: FF090_RS18940_lanthipeptide

1

PLFIGGKYAKADIVNSAESITTNFLCTCGTA

31

PLFIGG+YA+ADI+ SI+ CGTA

14

PLFIGGEYAEADIIGDTTSISGK----CGTA

40

33.3%

NZ_AP014940.1:

Lanthipeptides: LEN_RS25395_lanthipeptide

1

PLFIGGKYAKADIVNSAESIT--TNFLCTCGT

30

PLFIGG++A+ADI +E+I+ T+ CGT

13

PLFIGGEFAEADIAGDSEAISMITSVCTGCGT

44

33.3%

NZ_CP011130.1and 4 others:

NZ_CP023465.1 Lanthipeptides: CNO08_RS25510_lanthipeptideNZ_CP094357.1 Lanthipeptides: IEQ11_RS02795_lanthipeptideNZ_KQ061218.1 Lanthipeptides: WN54_RS23295_lanthipeptideNZ_LMHM01000017.1 Lanthipeptides: ASD86_RS21560_lanthipeptide

Lanthipeptides: LC55x_RS02840_lanthipeptide

1

PLFIGGKYAKADIVNS-AESITTNFLCTCGTAIHDNCC

37

PLFIGG+Y ++DI+ A +IT LCT ++ NCC

18

PLFIGGEYVESDIIGEVAPAITGCSLCT--GSMRINCC

53

32.1%

NZ_JAIQCM010000003.1:

Lanthipeptides: K9V56_RS08295_lanthipeptide

1

PLFIGGKYAKADIVNSAESITTNFLCTCGTA

31

PLFIGG+YA+ADIV +I+ CGTA

17

PLFIGGEYAEADIVGDTSTISGK----CGTA

43

MIBiG 4.0 matches for EKH80_RS03455

10.5%

Lanthipeptide: nisin A

27

TCGTAIH

33

TC +IH

25

TCHCSIH

31

10.3%

BGC0001229.4 (SBI_06990):

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

26

CTCGTAIHDNC

36

CT NC

28

CTLSKECQPNC

38

allorf_163957_164115

Leader:

MRRSGAMRKRTSQSEMTQRVGCIWCAEDLRAA

Core:

NRRNYETRLMHCASTRCVKE

Core with dehydrations:

NRRNYEDhbRLMHCysADhaDhbRCysVKE

antiSMASH-DB 4.0 matches for allorf_163957_164115

100.0%

Lanthipeptides: allorf_163957_164115_lanthipeptide

1

NRRNYETRLMHCASTRCVKE

20

NRRNYETRLMHCASTRCVKE

1

NRRNYETRLMHCASTRCVKE

20

allorf_175843_175911

Leader:

MSPQNALGQAAMARA

Core:

YTCLRGT

Core with dehydrations:

YDhbCysLRGDhb

antiSMASH-DB 4.0 matches for allorf_175843_175911

100.0%

Lanthipeptides: allorf_175843_175911_lanthipeptide

1

YTCLRGT

7

YTCLRGT

1

YTCLRGT

7

allorf_183483_183668

Leader:

MMLGVIRPSSLLHRRGEGRFSHGG

Core:

CMSQAMQPSTKCRNSTRAKGPLGFAPNGVFLSVHLDA

Core with dehydrations:

CysMDhaQAMQPDhaDhbKCysRNDhaDhbRAKGPLGFAPNGVFLDhaVHLDA

antiSMASH-DB 4.0 matches for allorf_183483_183668

100.0%

BGC0001229.4 (SBI_06989):

Lanthipeptides: allorf_183483_183668_lanthipeptide

1

CMSQAMQPSTKCRNSTRAKGPLGFAPNGVFLSVHLDA

37

CMSQAMQPSTKCRNSTRAKGPLGFAPNGVFLSVHLDA

1

CMSQAMQPSTKCRNSTRAKGPLGFAPNGVFLSVHLDA

37

24.3%

NZ_CP040417.1and 7 others:

NZ_CP040417.1 Lanthipeptides: FE328_RS09035_lanthipeptideNZ_CP040419.1 Lanthipeptides: FE326_RS02890_lanthipeptideNZ_CP040419.1 Lanthipeptides: FE326_RS02890_lanthipeptideNZ_CP040421.1 Lanthipeptides: FE324_RS02830_lanthipeptideNZ_CP040421.1 Lanthipeptides: FE324_RS02830_lanthipeptideNZ_CP040422.1 Lanthipeptides: FE323_RS02865_lanthipeptideNZ_CP040422.1 Lanthipeptides: FE323_RS02865_lanthipeptide

Lanthipeptides: FE328_RS09035_lanthipeptide

2

MSQAMQPSTKCRNSTR

17

+S A+ PSTKC + TR

9

ISLAICPSTKCASITR

24

24.3%

NZ_KV815277.1:

Lanthipeptides: allorf_240001_240225_lanthipeptide

1

CMSQAMQPSTKCRNSTRAKGPLGFAPNG

28

C+S A PST R + +P+G

2

CLSGAASPSTPTRECSECSRSYTDSPSG

29

with 3 more sequence matches

18.9%

NZ_KZ819159.1and 2 others:

NZ_KZ819159.1 Lanthipeptides: allorf_2152839_2153060_lanthipeptideNZ_KZ819159.1 Lanthipeptides: allorf_2152839_2153060_lanthipeptide

Lanthipeptides: allorf_2152839_2153060_lanthipeptide

17

RAKGPLGFAPN

27

RA+GP GF P+

5

RAEGPGGFLPS

15

16.3%

NZ_BNBV01000031.1and 3 others:

NZ_BNBV01000031.1 Lanthipeptides: IE286_RS22985_lanthipeptideNZ_BNBV01000031.1 Lanthipeptides: IE286_RS22990_lanthipeptideNZ_BNBV01000031.1 Lanthipeptides: IE286_RS22985_lanthipeptide

Lanthipeptides: IE286_RS22990_lanthipeptide

3

SQAMQPSTKCRN

14

S A P+TKC N

30

SAAFCPTTKCTN

41

16.3%

NZ_CP107567.1and 3 others:

NZ_CP107567.1 Lanthipeptides: OGH68_RS19755_lanthipeptideNZ_CP107567.1 Lanthipeptides: OGH68_RS19755_lanthipeptideNZ_CP107567.1 Lanthipeptides: OGH68_RS19755_lanthipeptide

Lanthipeptides: OGH68_RS19755_lanthipeptide

2

MSQAMQPSTKCRNS

15

+S AM P+TKC ++

29

VSAAMCPTTKCTSA

42

MIBiG 4.0 matches for allorf_183483_183668

16.2%

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

2

MSQAMQPSTKCRN

14

+S A P+TKC +

23

VSAAFCPTTKCTS

35

13.5%

BGC0001639.3 (OUH62395.1):

Lasso peptide: klebsidin

24

FAPNGV

29

F PNGV

10

FNPNGV

15

10.8%

BGC0002110.3 (QKV49894.1):

Lanthipeptide: birimositide

8

PSTKCRN

14

P+TKC +

18

PTTKCTS

24

allorf_183661_183744

Leader:

MLDVIPVG

Core:

GQVDGASKRATRLSTCHSG

Core with dehydrations:

GQVDGADhaKRADhbRLDhaDhbCysHDhaG

antiSMASH-DB 4.0 matches for allorf_183661_183744

100.0%

Lanthipeptides: allorf_183661_183744_lanthipeptide

1

GQVDGASKRATRLSTCHSG

19

GQVDGASKRATRLSTCHSG

1

GQVDGASKRATRLSTCHSG

19

26.3%

NZ_CP029194.1:

Lanthipeptides: allorf_0972442_0972567_lanthipeptide

10

ATRLSTCHS

18

AT+ +TCH

3

ATKWTTCHE

11

21.4%

NZ_LIWB01000026.1:

Lanthipeptides: AMK33_RS42645_lanthipeptide

3

VDGASKRATRLSTCHS

18

DG + +S+CHS

7

FDGCQWWSLTVSSCHS

22

with 2 more sequence matches

19.4%

NZ_CP073766.1:

Lanthipeptides: KD145_RS08730_lanthipeptide

3

VDGASKRATRLSTCHS

18

V+G + TR TC S

5

VEGGAIPVTRFITCLS

20

18.5%

NZ_CP059991.1:

Lanthipeptides: allorf_7879374_7879520_lanthipeptide

10

ATRLSTCHS

18

AT+ +TCH+

12

ATKWTTCHT

20

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (2):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 19.01 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 19.86 out of 26.06

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

EKH80_RS03455 - Class II

Cleavage pHMM score: -4.60

RODEO score: 0

Monoisotopic mass: 3895.9 Da

Molecular weight: 3898.4 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 3916.4 Da N = 1; 3934.4 Da N = 2

allorf_163957_164115 - Class II

Cleavage pHMM score: -6.40

RODEO score: 0

Monoisotopic mass: 2412.2 Da

Molecular weight: 2413.7 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 2431.8 Da N = 1

allorf_175843_175911 - Class II

Cleavage pHMM score: -6.90

RODEO score: 0

Monoisotopic mass: 776.4 Da

Molecular weight: 776.9 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 794.9 Da N = 1

allorf_183483_183668 - Class II

Cleavage pHMM score: -3.00

RODEO score: 0

Monoisotopic mass: 3810.9 Da

Molecular weight: 3813.4 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3831.4 Da N = 1; 3849.4 Da N = 2; 3867.4 Da N = 3; 3885.5 Da N = 4

allorf_183661_183744 - Class II

Cleavage pHMM score: -6.00

RODEO score: 0

Monoisotopic mass: 1839.9 Da

Molecular weight: 1841.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1859.0 Da N = 1; 1877.0 Da N = 2; 1895.1 Da N = 3; 1913.1 Da N = 4

TFBS definitions

Regulator	Description

NZ_RYYV01000002.1 - Region 3 - NRPS,transAT-PKS

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Location: 185,121 - 273,093 nt. (total: 87,973 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase synthase

EKH80_RS03560: riboflavin biosynthesis protein RibD

smcogs: SMCOG1240: riboflavin biosynthesis protein RibD

EKH80_RS03630: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

Transferases: acylase aminotransferase

EKH80_RS03525: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

EKH80_RS03640: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: dehalogenase synthase

EKH80_RS03560: riboflavin biosynthesis protein RibD

smcogs: SMCOG1240: riboflavin biosynthesis protein RibD

EKH80_RS03590: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

Ligases: ligase synthase

EKH80_RS03595: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

EKH80_RS03600: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

EKH80_RS03635: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Translocases: permease

EKH80_RS03540: phenylalanine-specific permease

smcogs: SMCOG1038: phenylalanine-specific permease

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.92	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.92	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002075.3	0.90	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001128.4	0.88	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.86	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001758.4	0.86	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.85	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.84	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001825.4	0.83	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001050.5	0.80	NRPS, PKS	thalassospiramide A	Tistrella bauzanensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.76	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.76	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001758.4	0.75	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002075.3	0.75	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001128.4	0.75	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.74	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.74	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.74	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.73	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001133.4	0.72	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.79 out of 21.84

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.13 out of 20.47

Confidence: medium

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 16.86 out of 18.57

Confidence: medium

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 23.69 out of 30.25

Confidence: medium

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 20.28 out of 23.43

Confidence: medium

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 18.25 out of 20.01

Confidence: medium

Additional weak hits (12):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.69 out of 21.55

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.63 out of 21.55

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.78 out of 20.01

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.29 out of 25.75

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.26 out of 32.74

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 21.71 out of 33.73

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.41 out of 29.38

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.53 out of 35.63

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 22.10 out of 30.98

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.40 out of 23.43

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.84 out of 25.88

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.32 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 3, location 185120 - 273093:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Phe - Phe - NMe-X) + (Ile) + (NMe-X) + (ccmal - ccmal - mal - Thr - Ala - Ala)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

EKH80_RS03595: Phe - Phe - X

Search NORINE for peptide: strict or relaxed

AMP-binding (475..865): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: LCQAFDAAFWEMAMVVAGEFNSYGPTEGTVGATL
Nearest Stachelhaus code(s):: Trp DGWAVASVCK (68% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (1521..1909): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: LSQAFDASVWEAVMATGGEVNAYGPTESSVCASI
Nearest Stachelhaus code(s):: Leu DAWLLGAVCK (62% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (2572..2969): X

nrpys: Ile, Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LDRSFDASTYEVFLWTGGEMHVYGPTESTTFATA
Nearest Stachelhaus code(s):: Ile or Val DAYFWGVTFK (91% 8Å match); Val DAYFWGVTFK (85% 8Å match); Ile DAYFWGVTFK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

EKH80_RS03600: Ile

Search NORINE for peptide: strict or relaxed

AMP-binding (470..866): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LNRSFDACTYEVFLWSGGEVNVYGPTETTTFATC
Nearest Stachelhaus code(s):: Ile DAYFWGVTFK (91% 8Å match); Ile or Val DAYFWGVTFK (88% 8Å match); Leu DAYFWGVTFK (85% 8Å match); Val DAYFWGVTFK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

EKH80_RS03605: X

AMP-binding (30..424): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LSYAFDASFAEMALVVGGEINAYGPTEITVDALI
Nearest Stachelhaus code(s):: Arg DAESVGAVDK (65% 8Å match)
Stachelhaus code match:: 80% (moderate)

EKH80_RS03635: Thr - Ala - Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (4163..4578): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LSTHFDFSVWEGNQIFGGEVNMYGITETTVHVTY
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (5260..5654): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: FWATFDLSIFEVNTNLAGEYNLYGPSEDTTYSTF
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (6315..6709): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: FWATFDLSIFEVNTNLAGEYNLYGPSEDTTYSTF
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

EKH80_RS03640: mal

PKS_AT (8..288): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 70.8%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 75.5
inactive: 51.8
Methoxymalonyl-CoA: 22.3
Methylmalonyl-CoA: 21.3
Isobutyryl-CoA: 15.3
Propionyl-CoA: 12.6
fatty_acid: 10.8
trans-1,2-CPDA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

TFBS definitions

Regulator	Description
ArgR	Regulator of arginine biosynthesis genes
BldD	Development and antibiotic global regulator
ColR	Phenol-responsive regulator
MexT	Global virulence regulator
ToxT	Regulator of MSHA biosynthesis
VqsM	Regulator of quorum-sensing signalling systems

NZ_RYYV01000026.1 - Region 1 - terpene

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Location: 33,377 - 52,734 nt. (total: 19,358 nt) Show pHMM detection rules used

Region on contig edge.

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

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Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: glucosidase

EKH80_RS21185: Alpha-glucosidase

smcogs: SMCOG1257: Alpha-glucosidase

EKH80_RS21195: Alpha-glucosidase

smcogs: SMCOG1257: Alpha-glucosidase

Lyases: cyclase

EKH80_RS21200: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Ligases: synthase

EKH80_RS21205: hydroxymethylglutaryl-CoA synthase

smcogs: SMCOG1043: hydroxymethylglutaryl-CoA synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001642.3	0.74	terpene	koraiol	Fusarium fujikuroi IMI 58289
BGC0002285.2	0.47	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0000550.3	0.38	ribosomal	salivaricin G32	Streptococcus salivarius
BGC0000545.5	0.38	ribosomal	ruminococcin A	[Ruminococcus] gnavus E1
BGC0001788.3	0.37	ribosomal	suicin 65	Streptococcus suis
BGC0000557.5	0.37	ribosomal	streptococcin A-FF22	Streptococcus pyogenes
BGC0000527.5	0.37	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0000547.5	0.36	ribosomal	salivaricin 9	Streptococcus salivarius
BGC0000188.5	0.36	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3
BGC0000506.4	0.35	ribosomal	entianin	Bacillus subtilis subsp. spizizenii

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001642.3	0.74	terpene	koraiol	Fusarium fujikuroi IMI 58289
BGC0002285.2	0.47	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0000550.3	0.38	ribosomal	salivaricin G32	Streptococcus salivarius
BGC0000545.5	0.38	ribosomal	ruminococcin A	[Ruminococcus] gnavus E1
BGC0001788.3	0.37	ribosomal	suicin 65	Streptococcus suis
BGC0000557.5	0.37	ribosomal	streptococcin A-FF22	Streptococcus pyogenes
BGC0000527.5	0.37	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0000547.5	0.36	ribosomal	salivaricin 9	Streptococcus salivarius
BGC0000188.5	0.36	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3
BGC0000506.4	0.35	ribosomal	entianin	Bacillus subtilis subsp. spizizenii

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene			C15	C1-C10	none

Genes with terpene domains:

EKH80_RS21200: T1TS

Domain 1 (22 - 325): Type 1 terpene synthase

Subtype: Corvol ether A and B synthase type

Reactions:: FPP → sesquiterpene_C1-C10

Transcription Factor Binding Sites

Binding sites found:

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 23.45 out of 27.55

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.31 out of 20.97

Confidence: medium

Additional weak hits (4):

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.94 out of 20.01

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.94 out of 20.01

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.94 out of 20.01

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 19.75 out of 27.78

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
FPP	Farnesyl diphosphate

TFBS definitions

Regulator	Description
CcpA	Catabolite control protein A
GnfM	Regulator of nitrogen-fixation genes

NZ_RYYV01000032.1 - Region 1 - NRPS

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Location: 1 - 32,883 nt. (total: 32,883 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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other genes

resistance

binding site

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Gene details

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Gene overview

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NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

EKH80_RS22220: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Hydrolases: esterase

EKH80_RS22285: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase

EKH80_RS22300: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000465.4	0.68	NRPS	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0000426.5	0.67	NRPS	sevadicin	Paenibacillus larvae
BGC0001133.4	0.65	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001758.4	0.65	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.65	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.65	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.65	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002071.2	0.65	NRPS	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001132.4	0.64	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001135.5	0.64	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000465.4	0.68	NRPS	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0000426.5	0.67	NRPS	sevadicin	Paenibacillus larvae
BGC0001133.4	0.65	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001758.4	0.65	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.65	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.65	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.65	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002071.2	0.65	NRPS	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001132.4	0.64	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001135.5	0.64	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

Additional weak hits (5):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.20 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.51 out of 26.06

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.34 out of 21.55

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 19.94 out of 30.25

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.66 out of 25.75

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 32883:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (NMe-Ser - D-Gly) + (D-Asp)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

EKH80_RS22295: Ser - Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (471..876): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFICSGEHNLYGPTEAAVDVTA
Nearest Stachelhaus code(s):: Ser DVWHISLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1948..2357): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: KAVSFDISLLELHALTGGDIELYGPTEAAIICAH
Nearest Stachelhaus code(s):: Gly DILQLGLIWK (59% 8Å match)
Stachelhaus code match:: 80% (moderate)

EKH80_RS22300: Asp - Thr

Search NORINE for peptide: strict or relaxed

AMP-binding (463..861): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWDFFDAGVTTGKLMCGGEINHFGPTETTVGCAT
Nearest Stachelhaus code(s):: Asp DATKMGHVGK (71% 8Å match); CysA DATKMGHVGK (68% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1984..2293): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LSTHFDFSVWEGNQIFGGEVNMYGITETTVHVTY
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator

NZ_RYYV01000035.1 - Region 1 - NRPS-like

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Location: 1 - 21,480 nt. (total: 21,480 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

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SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: aminotransferase

EKH80_RS22615: aminotransferase

smcogs: SMCOG1019: aminotransferase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001825.4	0.64	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001132.4	0.64	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.63	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.63	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002286.3	0.63	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.63	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.63	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.63	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.62	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001166.5	0.60	NRPS	HC-toxin	Alternaria jesenskae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001825.4	0.64	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001132.4	0.64	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.63	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.63	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002286.3	0.63	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.63	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.63	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.63	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.62	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001166.5	0.60	NRPS	HC-toxin	Alternaria jesenskae

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 24.28 out of 27.78

Confidence: strong

Additional weak hits (2):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.73 out of 26.06

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 19.83 out of 26.12

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

TFBS Finder

TFBS definitions

Regulator	Description
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria

NZ_RYYV01000038.1 - Region 1 - NRPS

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Location: 1 - 20,574 nt. (total: 20,574 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

EKH80_RS22930: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.72	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001133.4	0.71	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.70	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0002286.3	0.69	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000307.4	0.69	NRPS	AbT1	Aureobasidium pullulans
BGC0001132.4	0.69	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.72	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001133.4	0.71	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.70	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0002286.3	0.69	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000307.4	0.69	NRPS	AbT1	Aureobasidium pullulans
BGC0001132.4	0.69	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (1):

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.19 out of 35.63

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 20574:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Phe - Ile) + (D-Asp - X - Gly)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

EKH80_RS22930: Phe - Ile

Search NORINE for peptide: strict or relaxed

AMP-binding (714..1104): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: LSQAFDAAFWEMTLIVAGEFNAYGPTEATVCATV
Nearest Stachelhaus code(s):: Phe DAWTIAAVCK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1769..2158): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LNNGFDISTLEAHLLVGGDLHCYGPTESTTFATT
Nearest Stachelhaus code(s):: Ile DALFLGCTFK (65% 8Å match)
Stachelhaus code match:: 80% (moderate)

EKH80_RS22935: Asp - X - Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (462..860): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWDFFDAGVTTGKLMCGGEINHFGPTETTVGCAT
Nearest Stachelhaus code(s):: Asp DATKMGHVGK (71% 8Å match); CysA DATKMGHVGK (68% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1984..2577): X

nrpys: OH-Asn, Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWATFDATVTSLKLVVGGEINEYGPTETVVGCVA
Nearest Stachelhaus code(s):: OH-Asn or Asn DATKVGEVGK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3233..3633): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: FSMTFDIAGLELQALTGGEVNLYGPTETTVWSSL
Nearest Stachelhaus code(s):: Gly DILQLGLVWK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description

NZ_RYYV01000004.1 - Region 1 - lanthipeptide-class-ii

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Location: 98,541 - 121,363 nt. (total: 22,823 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: electron transfer oxidoreductase

EKH80_RS06625: dihydropteridine reductase

smcogs: SMCOG1159: dihydropteridine reductase

EKH80_RS06650: flavodoxin

smcogs: SMCOG1134: flavodoxin

Transferases: sulfotransferase

EKH80_RS06615: Sulfotransferase domain

extras: Sulfotransfer_3: Sulfotransferase domain

Hydrolases: dehalogenase hydrolase peptidase

EKH80_RS06635: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

EKH80_RS06640: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

EKH80_RS06680: methionine aminopeptidase

smcogs: SMCOG1172: methionine aminopeptidase

Lyases: cyclase

EKH80_RS06645: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000525.5	0.50	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0000528.5	0.45	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0001579.4	0.43	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0001660.3	0.43	ribosomal	mathermycin	Marinactinospora thermotolerans
BGC0001576.3	0.43	ribosomal	divamide A	Prochloron didemni
BGC0000503.5	0.42	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0000554.4	0.42	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0001364.3	0.41	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919
BGC0002346.3	0.40	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0001210.5	0.39	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000525.5	0.50	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0000528.5	0.45	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0001579.4	0.43	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0001660.3	0.43	ribosomal	mathermycin	Marinactinospora thermotolerans
BGC0001576.3	0.43	ribosomal	divamide A	Prochloron didemni
BGC0000503.5	0.42	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0000554.4	0.42	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0001364.3	0.41	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919
BGC0002346.3	0.40	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0001210.5	0.39	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for EKH80_RS06645

allorf_104369_104572

Leader:

MRIALFGKDRVGWGHHKCDKSGLSSLVIRRRHSAVTAM

Core:

PAWYRNTLRSRSTAGKGRNTCLRRQVHAC

Core with dehydrations:

PAWYRNDhbLRDhaRDhaDhbAGKGRNDhbCysLRRQVHACys

antiSMASH-DB 4.0 matches for allorf_104369_104572

100.0%

Lanthipeptides: allorf_104369_104572_lanthipeptide

1

PAWYRNTLRSRSTAGKGRNTCLRRQVHAC

29

PAWYRNTLRSRSTAGKGRNTCLRRQVHAC

1

PAWYRNTLRSRSTAGKGRNTCLRRQVHAC

29

30.6%

NZ_CABWKH010000001.1and 1 other:

NZ_CP010411.1 Lanthipeptides: allorf_1259634_1259786_lanthipeptide

Lanthipeptides: allorf_022146_022298_lanthipeptide

3

WYRNTLRSRST-AGKGRNTCLRRQVHAC

29

W+R L R T G GR+ C R +C

6

WHRAGLAVRETRMGDGRSACPRVAAGSC

33

27.6%

NC_019673.1:

Lanthipeptides: allorf_4537901_4538068_lanthipeptide

10

SRSTAGKGRNTCLRRQVHAC

29

SR G GR +R + AC

2

SRGPPGPGRTNTVRPRNAAC

21

with 1 more sequence matches

21.7%

NZ_LR881952.1and 4 others:

NZ_LR881952.1 Lanthipeptides: allorf_5341425_5341625_lanthipeptideNZ_LR881952.1 Lanthipeptides: allorf_5341425_5341625_lanthipeptideNZ_LR881952.1 Lanthipeptides: allorf_5341425_5341625_lanthipeptideNZ_LZQS01000002.1 Lanthipeptides: allorf_595585_595785_lanthipeptide

Lanthipeptides: allorf_5341425_5341625_lanthipeptide

1

PAWYRNTLRSRSTAGKG

17

PAW TLR R AG G

12

PAW--ETLRLRYGAGMG

26

allorf_104699_104878

Leader:

MGAHRKQDAAARTGIGASWSSCLIPEEHRSRGA

Core:

ARCLRTAHDPSIGHKGTPAPRYASAP

Core with dehydrations:

ARCysLRDhbAHDPDhaIGHKGDhbPAPRYADhaAP

antiSMASH-DB 4.0 matches for allorf_104699_104878

100.0%

Lanthipeptides: allorf_104699_104878_lanthipeptide

1

ARCLRTAHDPSIGHKGTPAPRYASAP

26

ARCLRTAHDPSIGHKGTPAPRYASAP

1

ARCLRTAHDPSIGHKGTPAPRYASAP

26

26.3%

NZ_FMXV01000009.1:

Lanthipeptides: allorf_148352_148558_lanthipeptide

3

CLRTA---HDPSIGHKGTPAP

20

CLRT H PS GH P

14

CLRTVGIGHCPSAGHNARRLP

34

21.9%

NZ_CP015405.2:

Lanthipeptides: allorf_4801575_4801745_lanthipeptide

1

ARCLRTAHDPSIGHK

15

RC TAH P G +

8

CRCADTAHFPEAGKE

22

with 1 more sequence matches

12.3%

NZ_LMEI01000006.1:

Lanthipeptides: allorf_386141_386362_lanthipeptide

3

CLRTAHDPSIGH

14

CLRT HDP+

27

CLRTVHDPATAQ

38

allorf_107615_107755

Leader:

MRHGANQVQAG

Core:

AGMTAWLSVVPSDRHWKPAGRTDIQPPCAKWSRFG

Core with dehydrations:

AGMDhbAWLDhaVVPDhaDRHWKPAGRDhbDIQPPCysAKWDhaRFG

antiSMASH-DB 4.0 matches for allorf_107615_107755

100.0%

Lanthipeptides: allorf_107615_107755_lanthipeptide

1

AGMTAWLSVVPSDRHWKPAGRTDIQPPCAKWSRFG

35

AGMTAWLSVVPSDRHWKPAGRTDIQPPCAKWSRFG

1

AGMTAWLSVVPSDRHWKPAGRTDIQPPCAKWSRFG

35

16.2%

NZ_CP023465.1and 1 other:

NZ_KQ061218.1 Lanthipeptides: allorf_237147_237311_lanthipeptide

Lanthipeptides: allorf_0704938_0705102_lanthipeptide

20

GRTDIQPPCAKWSRF

34

GR + P C++ +RF

14

GRNLLLPSCSRSARF

28

14.3%

NZ_CP102384.1and 1 other:

NZ_JAATIT010000001.1 Lassopeptides: allorf_0891587_0891718_lassopeptide

Lassopeptides: NP825_RS15870_lassopeptide

18

PAGRTDIQP

26

P+G+ D+QP

4

PSGQQDVQP

12

with 2 more sequence matches

14.3%

NZ_QLLX01000002.1and 1 other:

NZ_QLLX01000002.1 Lanthipeptides: allorf_335018_335257_lanthipeptide

Lanthipeptides: allorf_335018_335257_lanthipeptide

19

AGRTDIQPPCAKWSRFG

35

A RTD +PP SR G

16

APRTDRRPPAMSRSRTG

32

11.9%

NZ_CP072384.1:

Lanthipeptides: allorf_0337653_0337835_lanthipeptide

21

RTDIQPPCAKWSR

33

R+D+ PC++++R

1

RSDMSGPCSRFAR

13

allorf_108229_108468

Leader:

MLASVENQQQLQPEAQDCVVLNMVSILSA

Core:

LARVVIFFSVEPSTKSGPAGLPKPLMLSAPSSQAETTLSFCCIAELLRRS

Core with dehydrations:

LARVVIFFDhaVEPDhaDhbKDhaGPAGLPKPLMLDhaAPDhaDhaQAEDhbDhbLDhaFCysCysIAELLRRDha

antiSMASH-DB 4.0 matches for allorf_108229_108468

100.0%

Lanthipeptides: allorf_108229_108468_lanthipeptide

1

LARVVIFFSVEPSTKSGPAGLPKPLMLSAPSSQAETTLSFCCIAELLRRS

50

LARVVIFFSVEPSTKSGPAGLPKPLMLSAPSSQAETTLSFCCIAELLRRS

1

LARVVIFFSVEPSTKSGPAGLPKPLMLSAPSSQAETTLSFCCIAELLRRS

50

23.2%

NZ_FOGI01000013.1:

Lanthipeptides: allorf_142264_142503_lanthipeptide

7

FFSVEPSTKSG-PAGLPKPLMLSA--PSSQAETTLSFCCIAELLRRS

50

F S + T S PAG P PL + A P +++ C A L R+

16

FASPDRKTNSHLPAGSPPPLAVEACSPGGRSDWKEWITCKARLWWRA

62

allorf_108254_108448

Leader:

MQQNDNVVSAWLEGADSISG

Core:

LGNPAGPLFVDGSTEKKMTTRASAERIDTMFRTTQSCASGCSCC

Core with dehydrations:

LGNPAGPLFVDGDhaDhbEKKMDhbDhbRADhaAERIDDhbMFRDhbDhbQDhaCysADhaGCysDhaCysCys

antiSMASH-DB 4.0 matches for allorf_108254_108448

100.0%

Lanthipeptides: allorf_108254_108448_lanthipeptide

1

LGNPAGPLFVDGSTEKKMTTRASAERIDTMFRTTQSCASGCSCC

44

LGNPAGPLFVDGSTEKKMTTRASAERIDTMFRTTQSCASGCSCC

1

LGNPAGPLFVDGSTEKKMTTRASAERIDTMFRTTQSCASGCSCC

44

36.4%

NZ_CP023690.1and 1 other:

NZ_CP030771.1 Lanthipeptides: C9F11_RS41405_lanthipeptide

Lanthipeptides: CP982_RS40715_lanthipeptide

3

NPAGPLFVDGSTEKKMTTRASAERIDTMFR--TTQSCASGCSCC

44

NPAGPL+V GS + T S D+++ ++ S + G CC

3

NPAGPLYVGGSAAEAALTDPS----DSLYGFCSSPSGSHGSWCC

42

34.1%

NZ_BMNG01000007.1:

Lanthipeptides: IEY91_RS17560_lanthipeptide

3

NPAGPLFVDGSTEKKMTTRASAERIDTMFRTTQSCASGCSCC

44

NPAGPL+V G+ + T A+ F ++ S + G CC

3

NPAGPLYVGGTVGEAALTDATDSLYG--FCSSPSGSLGSWCC

42

with 47 more sequence matches

Only the first 10 are displayed here.

34.1%

NZ_BMUQ01000012.1and 11 others:

NZ_CP041604.2 Lanthipeptides: FNV64_RS07000_lanthipeptideNZ_CP041610.2 Lanthipeptides: FNV62_RS03705_lanthipeptideNZ_CP041611.1 Lanthipeptides: FNV63_RS03970_lanthipeptideNZ_CP041613.2 Lanthipeptides: FNV68_RS05855_lanthipeptideNZ_CP041650.2 Lanthipeptides: FNV59_RS05275_lanthipeptideNZ_CP050974.1 Lanthipeptides: HEP84_RS02235_lanthipeptideNZ_CP051010.1 Lanthipeptides: HEP87_RS03725_lanthipeptideNZ_FONR01000009.1 Lanthipeptides: BM303_RS25620_lanthipeptideNZ_KQ948493.1 Lanthipeptides: AQJ27_RS49365_lanthipeptideNZ_VISR01000021.1 Lanthipeptides: FB157_RS31330_lanthipeptideNZ_VISR01000021.1 Lanthipeptides: FB157_RS31330_lanthipeptide

Lanthipeptides: IE289_RS27575_lanthipeptide

3

NPAGPLFVDGSTEKKMTTRASAERIDTMFR--TTQSCASGCSCC

44

NPAGPL+V G+ + T S D+++ ++ S + G CC

3

NPAGPLYVGGAAAEAALTDPS----DSLYGFCSSPSGSHGSWCC

42

34.1%

NZ_BMUU01000006.1and 2 others:

NZ_BMUZ01000004.1 Lanthipeptides: IE267_RS12185_lanthipeptideNZ_CP070373.1 Lanthipeptides: GWQ54_RS15625_lanthipeptide

Lanthipeptides: IE233_RS19480_lanthipeptide

3

NPAGPLFVDGSTEKKMTTRASAERIDTMFR--TTQSCASGCSCC

44

NPAGPL+V G+ + T +S D+++ ++ S + G CC

3

NPAGPLYVGGAAAEAALTDSS----DSLYGFCSSPSGSHGSWCC

42

34.1%

NZ_BMVR01000007.1and 3 others:

NZ_CP040916.1 Lanthipeptides: FH965_RS39150_lanthipeptideNZ_MQUS01000030.1 Lanthipeptides: AV521_RS36555_lanthipeptideNZ_RHGC01000002.1 Lanthipeptides: EJ355_RS11575_lanthipeptide

Lanthipeptides: IE274_RS20785_lanthipeptide

3

NPAGPLFVDGSTEKKMTTRASAERIDTMFR--TTQSCASGCSCC

44

NPAGPL+V G+ + T S D+++ ++ S + G CC

3

NPAGPLYVGGTAAEAALTDPS----DSLYGFCSSPSGSHGSWCC

42

34.1%

NZ_CP016279.1and 1 other:

NZ_CP016279.1 Lanthipeptides: AVL59_RS19575_lanthipeptide

Lanthipeptides: AVL59_RS19575_lanthipeptide

3

NPAGPLFVDGSTEKKMTTRASAERIDTMFRTTQSCASGCSCC

44

NPAGPL+V G + T S F ++ S + G CC

3

NPAGPLYVGGRAAEAALTDPSDALYG--FCSSPSGSHGSWCC

42

34.1%

NZ_CP029194.1and 1 other:

NZ_CP059991.1 Lanthipeptides: H4W23_RS35675_lanthipeptide

Lanthipeptides: DEJ46_RS04155_lanthipeptide

3

NPAGPLFVDGSTEKKMTTRASAERIDTMFRTTQSCASGCSCC

44

NPAGPL+V G+ + T A T++ +GCS C

2

NPAGPLYVGGAAGEAALTDA-----------TEALLTGCSSC

32

34.1%

NZ_CP032698.1and 4 others:

NZ_CP106795.1 Lanthipeptides: N8I86_RS02690_lanthipeptideNZ_CP106795.1 Lanthipeptides: N8I86_RS02690_lanthipeptideNZ_LIWA01000005.1 Lanthipeptides: AMK32_RS23965_lanthipeptideNZ_LIWA01000005.1 Lanthipeptides: AMK32_RS23965_lanthipeptide

Lanthipeptides: DWB77_RS04745_lanthipeptide

3

NPAGPLFVDGSTEKKMTTRASAERIDTMFR--TTQSCASGCSCC

44

NPAGPL+V G+ + T S D+++ ++ S + G CC

3

NPAGPLYVGGTAAEAALTDPS----DSLYGFCSSPSGSRGSWCC

42

34.1%

NZ_CP098485.1and 4 others:

NZ_CP098485.1 Lanthipeptides: NAT22_RS36135_lanthipeptideNZ_CP106798.1 Lanthipeptides: N8I87_RS37365_lanthipeptideNZ_JACHJK010000003.1 Lanthipeptides: FHS34_RS11895_lanthipeptideNZ_JACHJK010000003.1 Lanthipeptides: FHS34_RS11895_lanthipeptide

Lanthipeptides: NAT22_RS36135_lanthipeptide

3

NPAGPLFVDGSTEKKMTTRASAERIDTMFR--TTQSCASGCSCC

44

NPAGPL+V G+ + T S D+++ ++ S + G CC

3

NPAGPLYVGGAAAEAALTDPS----DSLYGFCSSPSGSRGSWCC

42

34.1%

NZ_NNBJ01000008.1:

Lanthipeptides: CG740_RS17895_lanthipeptide

3

NPAGPLFVDGSTEKKMTTRASAERIDTMFR--TTQSCASGCSCC

44

NPAGPL+V G+ + T +S D+++ ++ S + G CC

3

NPAGPLYVGGAAAEAGLTDSS----DSLYGFCSSPSGSHGSWCC

42

34.1%

BGC0000480.5 (ACA04484.1 (core 1)):

Lanthipeptides: EKH80_RS03455_lanthipeptide

7

PLFVDGSTEKKMTTRASAERIDTMFRTTQSCASGCSCC

44

PLF+ G K SAE I T F T A +CC

1

PLFIGGKYAKADIVN-SAESITTNFLCTCGTAIHDNCC

37

32.7%

NZ_CP089414.1:

Lanthipeptides: DYST_RS04430_lanthipeptide

3

NPAGPLFVD--GSTEKKMTTRASAERIDTMFRTT------QSCASGCSCC

44

NPAGPL++ G+TE +T+ + + + T TT + GC+CC

3

NPAGPLYIQGAGATEAALTSDKTVKSMLTSVGTTCSTCSIHTSGGGCACC

52

MIBiG 4.0 matches for allorf_108254_108448

9.1%

Cyanobactin: tenuecyclamide A or tenuecyclamide C

38

ASGCSC

43

A+GC C

1

ATGCMC

6

allorf_108405_108521

Leader:

MMRRGDMEKA

Core:

NTRPDSAVCWPRWKISNNCSRKRRTALS

Core with dehydrations:

NDhbRPDDhaAVCysWPRWKIDhaNNCysDhaRKRRDhbALDha

antiSMASH-DB 4.0 matches for allorf_108405_108521

100.0%

BGC0000535.4 (ADJ56352.1):

Lanthipeptides: allorf_108405_108521_lanthipeptide

1

NTRPDSAVCWPRWKISNNCSRKRRTALS

28

NTRPDSAVCWPRWKISNNCSRKRRTALS

1

NTRPDSAVCWPRWKISNNCSRKRRTALS

28

22.2%

NC_017299.1and 1 other:

NC_017299.1 Lanthipeptides: H04402_RS03105_lanthipeptide

Lanthipeptides: H04402_RS03105_lanthipeptide

5

DSAVCWPRWKISNNCSRKR

23

D VC + NNCS K+

18

DGKVCTWTVECQNNCSHKK

36

21.4%

NZ_CP068102.1and 1 other:

NZ_CP068102.1 Lanthipeptides: allorf_1176509_1176580_lanthipeptide

Lanthipeptides: allorf_1176509_1176580_lanthipeptide

11

PRWKISNNCSRK

22

P++ NCSRK

2

PKYSAIKNCSRK

13

with 2 more sequence matches

15.2%

NZ_JANTYU010000003.1:

Lanthipeptides: allorf_298984_299208_lanthipeptide

12

RWKISNNCSRKRRTA

26

RW++S +C+ RR A

4

RWRMSIDCAVGRRGA

18

13.8%

NZ_RJUP01000016.1:

Lanthipeptides: allorf_085018_085254_lanthipeptide

6

SAVCWPRWKISNNCSRKRRTALS

28

S VC W +S NC + +S

30

STVCDSDWCLSFNCDSEGTFCIS

52

MIBiG 4.0 matches for allorf_108405_108521

8.8%

Lanthipeptide: nisin A

7

AVCWPRWK

14

++C P K

5

SLCTPGCK

12

allorf_118876_118977

Leader:

MLRLSGASSIFAA

Core:

AGRYPVKCPVLVMRVSSTVM

Core with dehydrations:

AGRYPVKCysPVLVMRVDhaDhaDhbVM

antiSMASH-DB 4.0 matches for allorf_118876_118977

100.0%

Lanthipeptides: allorf_118876_118977_lanthipeptide

1

AGRYPVKCPVLVMRVSSTVM

20

AGRYPVKCPVLVMRVSSTVM

1

AGRYPVKCPVLVMRVSSTVM

20

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.36 out of 19.26

Confidence: medium

Additional weak hits (4):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.93 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.66 out of 27.78

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.79 out of 22.40

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.67 out of 26.06

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

allorf_104369_104572 - Class II

Cleavage pHMM score: -6.30

RODEO score: 0

Monoisotopic mass: 3267.7 Da

Molecular weight: 3269.7 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3287.7 Da N = 1; 3305.7 Da N = 2; 3323.8 Da N = 3

allorf_104699_104878 - Class II

Cleavage pHMM score: -2.90

RODEO score: 0

Monoisotopic mass: 2657.4 Da

Molecular weight: 2659.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2677.0 Da N = 1; 2695.0 Da N = 2; 2713.0 Da N = 3

allorf_107615_107755 - Class II

Cleavage pHMM score: -3.00

RODEO score: 0

Monoisotopic mass: 3818.0 Da

Molecular weight: 3820.3 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 3838.4 Da N = 1; 3856.4 Da N = 2; 3874.4 Da N = 3; 3892.4 Da N = 4

allorf_108229_108468 - Class II

Cleavage pHMM score: -5.60

RODEO score: 0

Monoisotopic mass: 5106.8 Da

Molecular weight: 5110.0 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 5128.0 Da N = 1; 5146.0 Da N = 2; 5164.0 Da N = 3; 5182.1 Da N = 4; 5200.1 Da N = 5; 5218.1 Da N = 6; 5236.1 Da N = 7; 5254.1 Da N = 8; 5272.2 Da N = 9

allorf_108254_108448 - Class II

Cleavage pHMM score: -5.20

RODEO score: 0

Monoisotopic mass: 4429.1 Da

Molecular weight: 4432.0 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 4450.0 Da N = 1; 4468.0 Da N = 2; 4486.1 Da N = 3; 4504.1 Da N = 4; 4522.1 Da N = 5; 4540.1 Da N = 6; 4558.1 Da N = 7

allorf_108405_108521 - Class II

Cleavage pHMM score: -6.80

RODEO score: 0

Monoisotopic mass: 3193.7 Da

Molecular weight: 3195.6 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3213.6 Da N = 1; 3231.7 Da N = 2; 3249.7 Da N = 3; 3267.7 Da N = 4

allorf_118876_118977 - Class II

Cleavage pHMM score: -2.80

RODEO score: 0

Monoisotopic mass: 2138.2 Da

Molecular weight: 2139.6 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2157.7 Da N = 1; 2175.7 Da N = 2

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein

NZ_RYYV01000052.1 - Region 1 - NRPS

Download region SVG

Location: 1 - 5,727 nt. (total: 5,727 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.68	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000342.4	0.68	NRPS	enniatin	Fusarium equiseti
BGC0001135.5	0.67	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001261.3	0.66	NRPS	AM-toxin	Alternaria alternata
BGC0001825.4	0.66	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001758.4	0.65	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001132.4	0.65	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.65	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0000307.4	0.65	NRPS	AbT1	Aureobasidium pullulans
BGC0001128.4	0.65	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.68	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000342.4	0.68	NRPS	enniatin	Fusarium equiseti
BGC0001135.5	0.67	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001261.3	0.66	NRPS	AM-toxin	Alternaria alternata
BGC0001825.4	0.66	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001758.4	0.65	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001132.4	0.65	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.65	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0000307.4	0.65	NRPS	AbT1	Aureobasidium pullulans
BGC0001128.4	0.65	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 5727:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Val)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

EKH80_RS23515: Val

Search NORINE for peptide: strict or relaxed

AMP-binding (719..1117): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LHRSFDASTYEVWLIAGGDVNGYGPTETTTFATN
Nearest Stachelhaus code(s):: Val DAYWVGGTFK (88% 8Å match)
Stachelhaus code match:: 90% (moderate)

NZ_RYYV01000055.1 - Region 1 - transAT-PKS-like

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Location: 1 - 4,467 nt. (total: 4,467 nt) Show pHMM detection rules used

Region on contig edge.

transAT-PKS-like: cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS) and not PKS_AT)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

EKH80_RS23565: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000180.5	0.45	PKS	legioliulin	Legionella parisiensis
BGC0001050.5	0.42	NRPS, PKS	thalassospiramide A	Tistrella bauzanensis
BGC0000353.5	0.41	NRPS	tacrolimus	Streptomyces sp. MA6548
BGC0001109.4	0.39	NRPS, PKS	pederin	unidentified unclassified sequences.
BGC0001235.4	0.38	NRPS, PKS	antalid	Polyangium spumosum
BGC0000067.5	0.37	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0000185.5	0.37	PKS	tartrolon D, tartrolon E	Teredinibacter turnerae T7901
BGC0001795.3	0.36	PKS	swinholide A	Nostoc sp. UHCC 0450
BGC0001876.2	0.36	NRPS	thalassospiramide A	Thalassospira sp. CNJ-328
BGC0001301.5	0.36	PKS	anthracimycin	Streptomyces sp. T676

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000180.5	0.45	PKS	legioliulin	Legionella parisiensis
BGC0001050.5	0.42	NRPS, PKS	thalassospiramide A	Tistrella bauzanensis
BGC0000353.5	0.41	NRPS	tacrolimus	Streptomyces sp. MA6548
BGC0001109.4	0.39	NRPS, PKS	pederin	unidentified unclassified sequences.
BGC0001235.4	0.38	NRPS, PKS	antalid	Polyangium spumosum
BGC0000067.5	0.37	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0000185.5	0.37	PKS	tartrolon D, tartrolon E	Teredinibacter turnerae T7901
BGC0001795.3	0.36	PKS	swinholide A	Nostoc sp. UHCC 0450
BGC0001876.2	0.36	NRPS	thalassospiramide A	Thalassospira sp. CNJ-328
BGC0001301.5	0.36	PKS	anthracimycin	Streptomyces sp. T676

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed domain annotation

Selected features only

Show module domains

NZ_RYYV01000058.1 - Region 1 - NRPS

Download region SVG

Location: 1 - 2,825 nt. (total: 2,825 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

EKH80_RS23635: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

EKH80_RS23920: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.66	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.66	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000307.4	0.65	NRPS	AbT1	Aureobasidium pullulans
BGC0000342.4	0.64	NRPS	enniatin	Fusarium equiseti
BGC0001641.4	0.64	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.64	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.64	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001132.4	0.64	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.63	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001166.5	0.63	NRPS	HC-toxin	Alternaria jesenskae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.66	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.66	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000307.4	0.65	NRPS	AbT1	Aureobasidium pullulans
BGC0000342.4	0.64	NRPS	enniatin	Fusarium equiseti
BGC0001641.4	0.64	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.64	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.64	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001132.4	0.64	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.63	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001166.5	0.63	NRPS	HC-toxin	Alternaria jesenskae

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed domain annotation

Selected features only

Show module domains

NZ_RYYV01000059.1 - Region 1 - NRPS

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Location: 1 - 2,774 nt. (total: 2,774 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

EKH80_RS23645: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.66	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.66	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000307.4	0.65	NRPS	AbT1	Aureobasidium pullulans
BGC0001641.4	0.64	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.64	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.64	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.64	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.64	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.64	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.64	NRPS	icosalide A, icosalide B	Burkholderia gladioli

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.66	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.66	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000307.4	0.65	NRPS	AbT1	Aureobasidium pullulans
BGC0001641.4	0.64	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.64	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.64	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.64	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.64	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.64	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.64	NRPS	icosalide A, icosalide B	Burkholderia gladioli

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

NRPS/PKS substrate predictions

EKH80_RS23645: X

AMP-binding (422..669): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: QWFSFDAAVWELQW--------------------
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

NZ_RYYV01000006.1 - Region 1 - arylpolyene

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Location: 142,314 - 183,519 nt. (total: 41,206 nt) Show pHMM detection rules used

arylpolyene: (APE_KS1 or APE_KS2)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

EKH80_RS09910: 3-hydroxyisobutyrate dehydrogenase

smcogs: SMCOG1100: 3-hydroxyisobutyrate dehydrogenase

Transferases: acyltransferase transferase

EKH80_RS09975: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

EKH80_RS10115: acyltransferase

smcogs: SMCOG1141: acyltransferase

Hydrolases: dehalogenase

EKH80_RS10070: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

Isomerases: mutase

EKH80_RS10035: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Ligases: synthase

EKH80_RS10010: polyprenol-monophosphomannose synthase ppm1

smcogs: SMCOG1123: polyprenol-monophosphomannose synthase ppm1

EKH80_RS10020: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002191.2	0.56	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001217.5	0.50	other (other)	PreQ0 Base	Streptomyces qinglanensis
BGC0000840.5	0.48	other (other)	xanthomonadin I	Xanthomonas oryzae pv. oryzae
BGC0000255.5	0.44	PKS	pederin	Uncultured bacterium
BGC0002008.3	0.44	other (other)	aryl polyenes	Xenorhabdus doucetiae
BGC0001285.5	0.43	other (fatty acid)	pseudopyronine A, pseudopyronine B	Pseudomonas putida
BGC0000836.5	0.43	other (other)	APE Ec	Escherichia coli CFT073
BGC0001400.3	0.43	PKS	citreoviridin	Aspergillus terreus NIH2624
BGC0000837.4	0.42	other (other)	APE Vf	Aliivibrio fischeri ES114
BGC0001987.2	0.42	PKS	koreenceine A, koreenceine B, koreenceine C, koreenceine D	Pseudomonas koreensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002191.2	0.56	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001217.5	0.50	other (other)	PreQ0 Base	Streptomyces qinglanensis
BGC0000840.5	0.48	other (other)	xanthomonadin I	Xanthomonas oryzae pv. oryzae
BGC0000255.5	0.44	PKS	pederin	Uncultured bacterium
BGC0002008.3	0.44	other (other)	aryl polyenes	Xenorhabdus doucetiae
BGC0001285.5	0.43	other (fatty acid)	pseudopyronine A, pseudopyronine B	Pseudomonas putida
BGC0000836.5	0.43	other (other)	APE Ec	Escherichia coli CFT073
BGC0001400.3	0.43	PKS	citreoviridin	Aspergillus terreus NIH2624
BGC0000837.4	0.42	other (other)	APE Vf	Aliivibrio fischeri ES114
BGC0001987.2	0.42	PKS	koreenceine A, koreenceine B, koreenceine C, koreenceine D	Pseudomonas koreensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.92 out of 20.47

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.92 out of 20.47

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.59 out of 23.05

Confidence: medium

Additional weak hits (19):

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 19.20 out of 26.12

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.96 out of 21.55

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 20.19 out of 30.25

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.99 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 19.55 out of 26.06

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.12 out of 28.14

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.57 out of 27.78

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.58 out of 29.38

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 20.35 out of 31.73

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.04 out of 39.62

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 20.33 out of 30.25

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.93 out of 32.74

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 20.53 out of 30.25

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.43 out of 26.06

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 20.78 out of 33.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.35 out of 28.19

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.96 out of 35.63

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.27 out of 20.01

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 22.88 out of 30.98

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

TFBS Finder

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
AfsR	Pleiotropic regulatory for antibiotic production
VqsM	Regulator of quorum-sensing signalling systems

NZ_RYYV01000008.1 - Region 1 - RiPP-like

Download region SVG

Location: 14,573 - 25,430 nt. (total: 10,858 nt) Show pHMM detection rules used

Region on contig edge.

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001047.4	0.28	NRPS, PKS	syringolin A	Pseudomonas syringae
BGC0000495.5	0.23	ribosomal	actagardine	Actinoplanes garbadinensis
BGC0000807.3	0.20	saccharide	phosphonoglycans	Stackebrandtia nassauensis DSM 44728
BGC0001106.6	0.19	NRPS, PKS	oxazolomycin	Streptomyces albus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001047.4	0.28	NRPS, PKS	syringolin A	Pseudomonas syringae
BGC0000495.5	0.23	ribosomal	actagardine	Actinoplanes garbadinensis
BGC0000807.3	0.20	saccharide	phosphonoglycans	Stackebrandtia nassauensis DSM 44728
BGC0001106.6	0.19	NRPS, PKS	oxazolomycin	Streptomyces albus

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 16.82 out of 18.46

Confidence: medium

Additional weak hits (2):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.45 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 20.10 out of 29.38

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
RpoN	Regulator of nitrogen assimilation and virulence

NZ_RYYV01000008.1 - Region 2 - terpene