LOCUS       NZ_RYYV01000008        10858 bp    DNA     linear   CON 06-MAY-2024
DEFINITION  Dyella choica strain 4 M-K27 scaffold8, whole genome shotgun
            sequence.
ACCESSION   NZ_RYYV01000008
VERSION     NZ_RYYV01000008.1
KEYWORDS    WGS; RefSeq.
SOURCE      Dyella choica
  ORGANISM  Dyella choica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae; Dyella.
REFERENCE   1  (bases 1 to 203943)
  AUTHORS   Qiu,L.-H. and Gao,Z.-H.
  TITLE     Dyella dinghuensis sp. nov. DHOA06 and Dyella choica sp. nov.
            4M-K27, isolated from forest soil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 203943)
  AUTHORS   Qiu,L.-H. and Gao,Z.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) School of Life Sciences, Sun Yat-sen
            University, No. 135, Xinggang West Road, Guangzhou, Guangdong
            510275, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2018
            Assembly Method                   :: Velvet v. 1.2.10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 145.0x
            Sequencing Technology             :: Illumina Hiseq 4000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003977665.1-RS_2024_05_06
            Annotation Date                   :: 05/06/2024 01:53:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,733
            CDSs (total)                      :: 4,678
            Genes (coding)                    :: 4,648
            CDSs (with protein)               :: 4,648
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 9 of 30
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:37:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 14572
            Orig. end                         :: 25430
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            RYYV01000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10858
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10858
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10858
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5858](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5858
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            945..2312
                     /locus_tag="EKH80_RS12070"
                     /old_locus_tag="EKH80_12075"
     CDS             945..2312
                     /EC_number="6.3.2.2"
                     /GO_function="GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477658.1"
                     /locus_tag="EKH80_RS12070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH80_12075"
                     /product="glutamate--cysteine ligase"
                     /protein_id="WP_126685023.1"
                     /transl_table=11
                     /translation="MSIPSAVKSTPIYGRQQLIDYLAAGEKPRDAWLIGTEHEKFGFRT
                     EDLRPPAFEGERGIRALLEGIASRYGWEIAREGDTPVALMRDKANITLEPAGQLELSGA
                     PLETIHQTCLEVNTHLAEVRSVADELGLGFLGMGFQPKWPRDQMPWMPKGRYKIMREYM
                     PKVGSLGLDMMTRTCTVQVNLDFESEGDMVRKFRTSLALQPIATALFADSPFTDGKPNG
                     YLSYRSNVWTDTDADRTGMLDFVFEAGFGYERYVDYMLDVPMYFSYQDGRYVDLSGKDF
                     KRFMSGALDELPGTRATMKDWGDHLTTAFPEVRLKQYLEMRGADGGPWNRLCALPAFWV
                     GLLYDDEALHAAWDLVKDFSREERHALRDGVPKHALKLPFRRGTVRDLAREALQIAAHG
                     LKRRNRLNRNGANESIFLEPLIEIVDANQTPAERKLELFHGVWNGSVDPVFKEFAY"
     gene            2309..2947
                     /locus_tag="EKH80_RS12075"
                     /old_locus_tag="EKH80_12080"
     CDS             2309..2947
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908756.1"
                     /locus_tag="EKH80_RS12075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH80_12080"
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="WP_230473638.1"
                     /transl_table=11
                     /translation="MIATTPVQAPFLSGLLLGASLIIAIGAQNAFVLRQGLLRSHRFAV
                     ALFCALSDAALIAAGVGGLGVAIGQHPRMLAVVACAGALVLAWYGIKALRRALHPEVLT
                     PDTHGATASQWRVLAACAGFTWLNPHVYLDTVLLIGSLASAWPVPGPRTLFAIGAATAS
                     FTWFFGLAYGARLLAPLFRKPLAWRVLDVSIALVMWGIAGKLMLSFLAH"
     misc_feature    3019..3032
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: medium, score:
                     16.82"
                     /tool="antismash"
     gene            3112..3624
                     /locus_tag="EKH80_RS12080"
                     /old_locus_tag="EKH80_12085"
     CDS             3112..3624
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EKH80_RS12080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="EKH80_12085"
                     /product="hypothetical protein"
                     /protein_id="WP_126685024.1"
                     /transl_table=11
                     /translation="MIENRDEQFKELCAQYGAVMYLVQAFENEVANANSCITLAPGLKQ
                     PVTAGEEVEDPPSDEPFTVGLHTLVKRLSGLTFVPATLVSMLDQAKSRRDFLTKRFFRD
                     RAESFYMGRSEELLEELEGHGKFFHGAEKSLQDFLCPLLLKLGFAQAGVQGEFDMYFAA
                     PDSHMLH"
     gene            complement(3963..4424)
                     /locus_tag="EKH80_RS12085"
                     /old_locus_tag="EKH80_12090"
     CDS             complement(3963..4424)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465161.1"
                     /locus_tag="EKH80_RS12085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH80_12090"
                     /product="DoxX family protein"
                     /protein_id="WP_126685025.1"
                     /transl_table=11
                     /translation="MRHFLATTASRWLGILHAGRSLGPLVIRLVFGYFWLETGIGKVQN
                     LDGFTQRFIGWGIPHPAFNAALSAWTELVGGLLLMLGLFTRLVSIPMIINMLVALALVV
                     SSRLMGLDDYVEADEVLYITIFFWLLIAGPGKFSLDTLVARWLNIQTRD"
     gene            4668..4940
                     /locus_tag="EKH80_RS12090"
                     /old_locus_tag="EKH80_12095"
     CDS             4668..4940
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401640.1"
                     /locus_tag="EKH80_RS12090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH80_12095"
                     /product="hypothetical protein"
                     /protein_id="WP_126685026.1"
                     /transl_table=11
                     /translation="MNVKTVNASAMALMVAGLFAAATVSAAPAGAAAGDQAQVKCVNSS
                     SCKGQGACKQATNSCKGQNSCKGQGFTMQKTQADCEKAQAAAKAK"
     gene            5001..5858
                     /locus_tag="EKH80_RS12095"
                     /old_locus_tag="EKH80_12100"
     CDS             5001..5858
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465159.1"
                     /locus_tag="EKH80_RS12095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH80_12100"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_230473639.1"
                     /sec_met_domain="DUF692 (E-value: 7.2e-106, bitscore:
                     346.8, seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAMQSAERSDALPYLGYGLGLRVEHYEALRAQPSGVDWLEIVSEN
                     YMVQGGLPLTWLDRFRERFPLVMHGVSMSIGSTDPLDDDYLARLVTLARRIEPAWVSDH
                     LCWTGVQGVNLHDLMPLPYTEEALDHVVARVQQVQDRLKRRILLENVSSYVSYADSQLT
                     EWEFLAEVAGRADCMILLDINNVHVSAKNHGFSALDYLHGIPVSRVQQFHLAGYEQGER
                     LIIDTHDAPVSNAVWDLYTEAVRRFGRVSTMIERDDHFPPLAELKAELEHARMLAEPLL
                     RDAA"
     gene            5855..6634
                     /locus_tag="EKH80_RS12100"
                     /old_locus_tag="EKH80_12105"
     CDS             5855..6634
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /locus_tag="EKH80_RS12100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH80_12105"
                     /product="DNA-binding domain-containing protein"
                     /protein_id="WP_126685027.1"
                     /transl_table=11
                     /translation="MTSLQATQQQMLQAVLAESAQIPPIIRGSDLTDARSRLEIYRHGY
                     RVRLRDALQAEFSGLRSMAGARFEVMLDKYISAHPSEHYNIRWYGAGLAAFLDYARPWR
                     DQPQFGDMARLDWAISVAFDAADETSASAADLASVPPDAWPRLQLSLLRNLHVLDCRCN
                     VDAFRRAADHGHPRPRLRRLAPARQILVWRKETTVHYRRLDDTEWQVLSAALRGETLAG
                     LCECLAARHDGAAAMAHMVTLLQGWLSAGLIHGWTFA"
     misc_feature    6821..6839
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.45"
                     /tool="antismash"
     misc_feature    complement(6824..6839)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 20.1"
                     /tool="antismash"
     gene            6863..7765
                     /locus_tag="EKH80_RS12105"
                     /old_locus_tag="EKH80_12110"
     CDS             6863..7765
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1287: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /locus_tag="EKH80_RS12105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH80_12110"
                     /product="AraC family transcriptional regulator"
                     /protein_id="WP_126685028.1"
                     /transl_table=11
                     /translation="MYVDAYAKPTQASRPPTVAPALEALLNQSMLRVEVVAEPKFCGSW
                     FADAPCNGHGLFHLVGSGECLIESESLAETWHLQAGDLVVFAHGDPHRLRARDAGADID
                     TSLICGELRYASHTRHPLSHALPACFVVRDAQAHTLFRHLSTMMVEIAKTEVPGRKVLL
                     NKLADTLFTLAVCDYAHRHADQQGLFAALADNRICKVLQAVHEEPGRLWTMQNMAALAC
                     MSRSSFAERFAQLMNVPPMQYVTQWRVNVAERLLHDPEMSVASIAERLGYSSEAAFRRL
                     FKRVSGICPGRIRSGSRHA"
     gene            7998..8780
                     /locus_tag="EKH80_RS12110"
                     /old_locus_tag="EKH80_12115"
     CDS             7998..8780
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466668.1"
                     /locus_tag="EKH80_RS12110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH80_12115"
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="WP_230473640.1"
                     /transl_table=11
                     /translation="MATFRSGRDGATKTARPVLARRIRSGAGPMAARNLHGHVVQVLGQ
                     RIVSGAIKQGEVLPPEATMAEEMDVSRTSLREAMKVLSAKGLVEARPKVGTRVRDARFW
                     SQLDADVLAWRCASMPTSDFVQKLSEMREIIEPAAAASAARHRSVTQLKRMQVAFDAME
                     ASHDPESWTTADIDFHEAILAATGNELLISLFSVIETALSSYFTMSAHTAVNFKYSLPQ
                     HRTVLQAIRDKDPEAAHKAMVKVISDTKENLSSKRRKA"
     gene            complement(8940..10091)
                     /locus_tag="EKH80_RS12115"
                     /old_locus_tag="EKH80_12120"
     CDS             complement(8940..10091)
                     /EC_number="5.1.3.-"
                     /GO_function="GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA]"
                     /GO_function="GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081205.1"
                     /locus_tag="EKH80_RS12115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH80_12120"
                     /product="aldose epimerase family protein"
                     /protein_id="WP_230473641.1"
                     /transl_table=11
                     /translation="MKSAGLLGSIFTATVLLPLVATAGQATRASFGHTADGQEVEIVTL
                     TNSHGMRARIMSYGASLQSLLVPDRDGKLADVVLGYDTLQGYLDRRQYFGSTVGRYANR
                     IAHGKFTLDSKSYSLGLNDGTNTLHGGTKGFDVQVWKVLEVKPGPTPGVMMEYVSPDGD
                     QGYPGTLTVKATYALSDDNQLKIAYTATTDKPSIVNLSNHTYWNLGGEGSGSVMEQELM
                     IPGDAITPVSDAAAIPTGAIVSVSGTPYDFRVAKPIGRDIRDGRSQQLLFGHGYDMNWV
                     LSRKEIEPRVVARAEDPHSGRVMTIWSGKPGLQFDSGNFLDGATVGKDHHIYRQGDAFV
                     LEPQLFPDTPNHPDFGSARLAPGETYRNVIVYRFGIDQAAKQQ"
ORIGIN
        1 tcggcaaccg caatcgctgc aaccgttgcc ggtggcgcct gtacccagcg aagtgcgccc
       61 attggtgcaa tcgctcaata ccctgctggg caccgtgcgc aatgccgcgc aagctcagca
      121 gcacttcctt accaatgccg ctcatcaact acgcacgccg ctctccggct tgaaagcgca
      181 gctggaagta ttggctggcg aaacccgcga tggagcgcag caggaacgca tcgcacgtct
      241 gcacgacagc gtggatcgcc tggcgcacac tgccaatcag ctgcttgctc ttgcgcgtgc
      301 cgagccttcg gcgcacagcc caggcgactt caccgaggtc cagctggaag cactgatcgg
      361 cacggtggcc gactccatgc tcgatcgcgc tatagcgcgc aacatcgatc tgggcgcgga
      421 gtgcgaaccg gcgcacgttc atggcgtcct ttggctgctg cacgagctgt tgatcaacct
      481 cctcgacaac gccatccgtc acacgcccga ggacggccgc atcacgctgc gctgcggatt
      541 gcatggcggc gcgccctacc tggaagtgga ggacaacggc cccggcattc ccatctcaga
      601 gcgcgaacgc gtgcgcgaac gtttctatcg tgcggccggc agcgatggcc agagcagcgg
      661 gctggggctg gcgatcgtcg aggagatcgc tcgcagccac cacgcgcggt tcgaaatcct
      721 cgatgcgcgg gtagggacgg gggcgcgaat gcgcgtggcg tttccgtccg aaggcatgaa
      781 ggaacaggta ttgactgcgt agcccagggg gtttttgcta tttcctgtcc ccgcatgctc
      841 cctggctgta gctgtactgt cacgtacaaa ttttttcaca tacccccttt tcgcgacgat
      901 gcgcagcctc catagtcggg ggctatcccc gtgaggcttg ccccgtgtcc atacccagtg
      961 ccgtcaaaag caccccgatc tacggccgcc agcaactgat cgattacctg gccgccggcg
     1021 agaagccgcg tgatgcctgg ctgatcggca ccgagcacga aaaatttggc ttccgtacag
     1081 aggatcttcg cccaccggcg ttcgagggtg agcgcggcat acgcgcactg ctggaaggca
     1141 ttgcctcacg ttatggttgg gagattgccc gcgaaggcga taccccggtg gcgctgatgc
     1201 gcgacaaggc caacatcacg ctggagccgg ccggccagct ggagttgtcc ggcgcgccac
     1261 tggaaaccat ccaccagacc tgtctcgaag tgaacacgca tctggcagaa gtgcgctccg
     1321 tagccgacga gctgggcctg ggcttccttg gcatgggctt ccaaccgaag tggccccgtg
     1381 accagatgcc gtggatgccc aagggccgct acaagatcat gcgcgaatac atgcccaagg
     1441 tcggctcgct cggcctggac atgatgacgc gcacctgcac cgtgcaggtc aacctggatt
     1501 tcgagagcga aggcgacatg gtgcgcaagt tccgcaccag cctcgccttg caaccgatcg
     1561 ccaccgcgct tttcgccgat tcgccattta cggacggcaa gcccaacggc tatctctcct
     1621 accgctcgaa tgtgtggacg gataccgatg cggatcgtac cggcatgctc gacttcgtat
     1681 tcgaagcggg tttcggttac gaacgctatg tcgactacat gctggacgta ccgatgtatt
     1741 tcagctatca ggacggccgc tacgtcgacc tgtccggcaa ggacttcaag cgtttcatgt
     1801 caggcgcgct cgacgaactg cccggcacgc gcgccacgat gaaggactgg ggcgatcatc
     1861 tgaccaccgc ctttccggag gtacggctga agcaatacct ggaaatgcgt ggcgcggacg
     1921 gtggtccgtg gaatcgcctt tgtgcgcttc cggcgttctg ggtcggcctg ctctacgacg
     1981 atgaagcgct gcatgcagcg tgggatctgg tgaaggattt cagccgcgaa gaacgccatg
     2041 ctttgcgcga tggcgtgccc aagcatgcac tgaagctgcc attccgccgc ggcaccgtgc
     2101 gcgacctggc ccgcgaggcg ttgcaaatcg ctgcgcatgg cctgaaacgc cgcaaccggc
     2161 tcaatcgcaa cggcgcgaac gagagcatct tcctggaacc gctgattgaa atcgtcgacg
     2221 ccaatcagac gcccgccgaa cgcaagctag agctgttcca cggtgtgtgg aacggcagcg
     2281 tggatcccgt cttcaaggaa ttcgcctatt gatcgcgaca acgcccgttc aggcaccttt
     2341 cctttccgga ttgctgcttg gcgcctcgct gatcatcgcc attggcgcgc agaacgcctt
     2401 cgtgctgcgg caaggactgt tgcgatcgca ccgctttgcg gtggcgctgt tttgcgcact
     2461 ttctgacgcg gcattgattg cggcgggcgt cggcgggctg ggtgtcgcca tcggccagca
     2521 cccgcgcatg ctggccgtcg tggcttgtgc aggcgcgttg gtactggcgt ggtatggcat
     2581 caaggcactg cgccgcgcct tgcatcccga ggtgcttaca ccggacacgc acggcgccac
     2641 tgccagccag tggcgggtgc ttgccgcctg tgccggcttc acctggctca atccgcacgt
     2701 ttacctggat accgtgctgc tgatcggcag cctcgcctcg gcctggccgg taccgggacc
     2761 gcgcacgctg ttcgccatcg gcgcggcaac ggcttcgttc acctggttct tcggtctggc
     2821 ctatggtgcg cgcttgctcg cgccgctgtt ccgcaaaccg ctggcatggc gcgtgctgga
     2881 tgtgtcgatc gcgctggtga tgtggggtat tgccggcaag ctaatgcttt ccttcctggc
     2941 gcattgacgt catcgcctgg acgcgactcg ggcggaacat ctcaggcggc gatgggcatc
     3001 cctgccgccc aagatcttgg cacacaggtt gcttcggata gagcgaggca cgggtcgacg
     3061 caatcccatg cgccggtgat cgtttgatga cctgacgaga ggatcatgca tatgatcgag
     3121 aaccgcgacg agcaattcaa agagttgtgc gcccagtacg gtgccgtcat gtatctcgta
     3181 caggcattcg agaacgaagt ggctaacgcc aattcatgca tcactcttgc gccgggcctc
     3241 aagcagccag tcacggcggg ggaagaggtg gaagatccgc cgtcggacga gccatttacg
     3301 gtcggcctgc acaccctggt caagcgcctg agcgggctca cgtttgtgcc agcgacgctc
     3361 gtaagcatgc tcgaccaggc gaaatctcgc cgggacttcc tgaccaaacg attctttcgc
     3421 gatcgcgcgg aaagcttcta catgggcagg agcgaagagt tgctggaaga attggaaggg
     3481 cacggaaaat tctttcacgg cgcggagaag tcgctgcagg atttcctgtg tccgctactt
     3541 ctgaaactgg gctttgccca ggcgggcgta cagggagaat tcgacatgta cttcgctgcg
     3601 ccggactccc acatgctcca ttagggacgg tctgaaaaag tccgtcacgg acttccgcgc
     3661 cgcgtccggt acacgcccgg acgcggctat gccaaatcaa gcgcttacgc acctgtttcg
     3721 cggcgggcct tccctggccc gcggaaactc tgaactcatt cagagtttcc ttagctgccg
     3781 caaagcggca gtgccttttt ggccccccgg cgtcacgtcg cggcgcccgt gcaccccatc
     3841 ctgccgacta tgcaggaacg gcccgcacac ataggctgtg atgaccatcc cagcatcgag
     3901 cgcagtgcat gcatggccat gccggggttt gcaccccggc ttacgatggt ccccagcgca
     3961 catcaatcgc gcgtttgaat attcagccag cgcgcgacca gcgtgtccag gctgaacttg
     4021 cccggccccg ctatcaggag ccagaagaaa atcgtgatgt agagcacttc gtcggcttca
     4081 acgtagtcat ccaaccccat caggcggctc gataccacca gggccaaggc caccagcatg
     4141 ttgatgatca tcggaatgct gaccaggcgc gtgaacagtc cgagcatcag cagcaggccg
     4201 ccaaccagct ccgtccaggc cgacaaagct gcattgaacg ccgggtgagg aatgccccag
     4261 ccgatgaagc gctgggtaaa accgtccagg ttctgcacct tgccgatacc cgtctccagc
     4321 cagaagtaac cgaacaccag ccttatcacc aaggggccca gcgagcgtcc cgcatgcagg
     4381 atgccgagcc aacgcgaagc tgtcgtcgcc aagaaatgac gcattgcacc tattcccaca
     4441 cgtggagtag gcgcatgatg cgcgggctgc cccattgcct caatgtccag acgtccagac
     4501 tttcttgcct ggcatccgcc ggactgttgg gcataaatgc aggatgcacg gatgcagcga
     4561 ccgatggagc gaagcagcac aatcgccctt gtccgggtgg cctcatggca ccgggcaagt
     4621 atccctgtgc cgccctgcgg cgacccacaa atcttggagc gatagatatg aacgtcaaga
     4681 cagtcaatgc cagcgccatg gccctgatgg tggccggttt gttcgccgcc gccacggttt
     4741 ccgccgcgcc tgccggtgcc gccgccggcg accaggccca ggtcaagtgc gtgaactcca
     4801 gctcttgcaa gggccagggc gcctgcaagc aggccaccaa ttcctgcaag ggccagaact
     4861 cgtgcaaggg tcaaggcttc accatgcaga agacccaggc cgattgcgaa aaggcgcagg
     4921 ccgcagccaa agcgaagtga tgcacacggc gggatgcacg cacgccgcat gcgtcccgcc
     4981 ttctccagga gactctcgcc atggccatgc aatccgccga gcgctccgat gccttgccct
     5041 acctgggcta cggattgggc ttgcgcgttg agcactacga agcgctgcgt gcccagccat
     5101 ccggtgtcga ttggctggag attgtttcag aaaattacat ggtgcagggc ggcttgccgc
     5161 tgacctggct ggaccgcttc cgcgaacggt ttccgctggt gatgcatggc gtgtcgatgt
     5221 cgatcggcag caccgacccg ctggacgacg attacctggc ccgccttgtc acgctggcca
     5281 ggcgcatcga accggcctgg gtttccgacc acctttgctg gaccggcgtg cagggcgtga
     5341 atctgcacga cttgatgccg ctgccctata ccgaggaagc cctcgaccat gtcgttgccc
     5401 gtgtccaaca ggtacaggac cggctcaagc gccgcatttt gctcgaaaac gtttccagct
     5461 atgtcagcta tgccgattca cagctgaccg agtgggagtt tctggccgaa gtggccggac
     5521 gcgccgattg catgatcctg ctggacatca acaacgtgca tgtcagcgcg aagaaccatg
     5581 ggttttcggc gctggattac ctgcacggca ttccggtttc gcgcgtgcag cagtttcacc
     5641 tagccggcta cgaacagggc gagcgtttga tcatcgatac ccatgacgcg ccggtttcca
     5701 acgcagtgtg ggacctgtac accgaagccg tgcgtcggtt tggccgcgta tcgacgatga
     5761 tcgaacgcga tgaccatttc ccaccgctgg ccgaattgaa ggcggaactg gaacatgcgc
     5821 gcatgctcgc cgaaccgctc ttgcgagatg ccgcatgacc tcgctgcagg ccacgcaaca
     5881 gcagatgctg caggccgtgt tggcggaatc ggcacagata ccgccgatca ttcgtggcag
     5941 cgatcttacc gatgcccgta gccgcctgga gatctaccgg cacggctacc gtgtgcgtct
     6001 gcgcgacgcc ttgcaagccg aattctccgg gctgcgcagc atggccggcg cgcgcttcga
     6061 agtcatgctg gacaaataca tcagcgcaca cccttccgag cactacaata ttcgctggta
     6121 cggcgccggc cttgccgcct ttctcgacta tgcccgtccg tggcgcgacc agccccagtt
     6181 cggggacatg gcgcgactgg actgggcgat ttccgtcgcc ttcgacgcag ccgatgaaac
     6241 cagcgcaagc gccgccgatc tcgccagcgt accgccggat gcatggccgc ggctgcagct
     6301 ttccctgctg cgtaacctgc atgtgctgga ctgccgctgc aatgtcgatg cattccgccg
     6361 ggcagcggac catggacatc ctcggccacg cctgcgcagg ctcgcgccgg cgcggcagat
     6421 cctggtatgg cgcaaggaaa ccaccgtgca ctaccggcgc ctggacgaca ccgaatggca
     6481 ggtgctgagc gccgccctgc ggggtgaaac cttggctggc ctgtgcgaat gcctggccgc
     6541 gcgccacgat ggtgcagccg ccatggcgca catggtgacg ttgctgcagg gctggctgtc
     6601 agccggactg atccatggct ggacattcgc ctgagcgaac gctgctgcct tctgattgat
     6661 gtggcagtgc cgcaaccgct agccggctgt ttagaaacag ccggctagcg cttgatttaa
     6721 gcgtagccaa gtacggaccc gtgccggact tggcgcgttg agaaaggcag ggaagccctt
     6781 tctcaacatt ctgcttcagc acgtaacccg agccgacttc ccggcgaatg attgtcaaag
     6841 cgcagtccaa ggaagtctgt ccatgtacgt cgatgcttac gccaaaccaa cccaggcatc
     6901 acgaccgcct accgtcgcgc cggccctgga agccctgctc aaccagtcga tgctgcgtgt
     6961 cgaagtggtg gcggagccga aattctgcgg ttcctggttc gccgatgcgc cgtgcaatgg
     7021 ccatggactg ttccatctgg tgggttcggg cgaatgcctg attgaaagcg aatccctcgc
     7081 cgaaacctgg catctgcaag ccggagacct ggtggtgttt gcgcatggcg acccgcatcg
     7141 gctgcgtgct cgcgacgccg gggccgatat cgacaccagc ctgatctgtg gcgaacttcg
     7201 ttacgccagc cacactcgtc accctctcag ccatgccttg ccggcttgct ttgtggtgcg
     7261 cgacgcacaa gcccacaccc tgttccgcca tctgtcgacg atgatggtcg agatcgcgaa
     7321 aaccgaggtg ccgggccgca aggtgctgct gaacaagctg gccgatacgc tgttcacgct
     7381 cgccgtatgc gattacgccc accgccacgc cgaccagcag ggcctgttcg ccgccctggc
     7441 cgacaatcgc atctgcaagg tgctgcaggc cgtacacgaa gagcccggac gcctgtggac
     7501 gatgcagaac atggctgccc tggcctgcat gtcgcgctcg tcatttgccg aacgcttcgc
     7561 gcaactgatg aacgtaccgc ccatgcagta cgtcacacag tggcgcgtca atgttgcgga
     7621 acgtctgctg catgaccctg aaatgtcggt ggcgtcgatc gccgagcggc tggggtatag
     7681 cagcgaggcc gccttccgcc gcctgttcaa acgcgtgagc ggcatttgcc ccggccgcat
     7741 ccgctcgggc agccgtcacg cctaagcatt ccatccaggt gccggctcga ttccgccgtg
     7801 gtcgcggcac gacttggcac gaggcaaaaa catcctgccc ggccgcagcc tctagtctcc
     7861 ttcctgaaac gtggcatcat ttcccacggc gtgttgaccg gcgccgcgga ttcacgttgt
     7921 cctgctgcga ttagtgaacg atcaggtttt ctatcgggca ggtctttcca tggggtggga
     7981 ttggaggaga cggcagcatg gcaacctttc gaagtggcag ggatggggcc accaagactg
     8041 cccgtcctgt gctcgcgcgg cgcatccgca gcggcgccgg ccccatggcc gcgcgcaacc
     8101 tgcatgggca cgtagtgcag gtccttggac agcgcatcgt cagcggcgcg atcaagcagg
     8161 gcgaggtact gccgcccgaa gcgaccatgg ccgaagaaat ggacgtgagc cgaacctccc
     8221 tgcgcgaggc catgaaggtg ctctcggcca aaggcctggt ggaagcgcgc cccaaggtcg
     8281 gcacgcgcgt gcgcgatgcg cggttctgga gtcagctgga tgccgacgta ctcgcctggc
     8341 gctgcgcctc gatgcccact tcggacttcg tgcagaagct gtcggaaatg cgcgaaatca
     8401 tcgagccggc ggccgcggcg agcgccgcac ggcatcgcag cgtcacgcaa ttgaagcgca
     8461 tgcaggtcgc cttcgatgcc atggaagcct cacacgatcc ggagtcatgg accactgccg
     8521 acatcgactt ccacgaggcg attctggccg ccaccggcaa cgaactgctg atctcgctgt
     8581 tctcggtgat cgaaacggcg ctcagcagct acttcacgat gtcggcgcat acggccgtga
     8641 acttcaaata ttcgctgccg caacaccgca cggtgctgca ggccatccgc gacaaagatc
     8701 cggaagcagc ccacaaggcc atggtcaagg tgatttcgga tacgaaggaa aacctgagca
     8761 gcaagcggcg caaggcgtga gttggaacga agatgctgcc atcacggccc tgggtaaatt
     8821 ttgctgagta cagccgcgta ggctgggatg acaatcccag cttttggttc ggcattgcaa
     8881 atgctgggat tgtcatccca gcctacgcgg cttagctgcg ccctaaccgc cggctcgact
     8941 cactgttgct tcgcggcctg gtcgataccg aaacgataaa cgatgacgtt gcgataggtt
     9001 tcgcccggcg ccagccgcgc ggagccgaag tccggatgat tgggcgtgtc tggaaacagc
     9061 tgcggctcca gcacgaaggc atcgccctgg cgatagatat ggtgatcctt gcccacggtg
     9121 gcgccgtcaa ggaagttgcc cgaatcgaat tgcaggcccg gcttgcccga ccagatggtc
     9181 atcacccggc ccgaatgcgg atcctccgcc cgcgccacca cgcgcggctc gatttccttg
     9241 cggctgagca cccagttcat gtcgtatccg tggccgaaca gcagctgttg cgagcggcca
     9301 tcgcgaatgt cgcggccgat cggcttggcg acgcgaaaat cgtacggcgt gcccgatacg
     9361 gagacgattg ctcccgtcgg tatcgcagcg gcatcgctca ccggcgtgat cgcatcgccg
     9421 gggatcatca gttcctgctc catcacggag cccgagcctt caccgcccag gttccaatag
     9481 gtgtgattgg ataagttgac gatgctgggc ttgtccgtag tcgcggtgta ggcaatcttc
     9541 agctggttgt cgtcgctcaa ggcgtaggtc gccttcaccg tcaaggtgcc gggataaccc
     9601 tggtcgccat cgggactgac gtattccatc atcacgccgg gcgtcggacc cggctttacc
     9661 tcgagcacct tccacacctg cacgtcgaag cctttcgtgc cgccatgcag ggtattcgtg
     9721 ccgtcattca agccaaggct gtagctcttg ctgtccagcg tgaatttgcc gtgcgcgatg
     9781 cggttcgcat agcggccaac ggtcgaaccg aaatactggc gccggtcgag atacccctgc
     9841 agcgtgtcgt agccgagtac tacgtcggcg agcttgccgt cgcggtccgg caccaacaag
     9901 gactgcagcg acgcgccata gctcatgata cgcgcgcgca tgccatggct attggtaagc
     9961 gtcacgattt ccacctcctg cccatccgcc gtgtggccga atgaggcacg cgtcgcctgg
    10021 ccggctgtcg cgaccagcgg caacagcacg gttgccgtaa agatcgagcc gagcagccct
    10081 gcagatttca agcaactccc tttatgaaat cggccagcca agggccctgt cgcttctgag
    10141 catattgtat gattatatag acgcatggcc gtccgggctt tacgcgccgc agcacgggct
    10201 tcgaacaagc agaaccggga catgatcgtg gcaaggggca tgagcggagg gtgaccaaag
    10261 gcgaagtgga aaagacgcat gttataggcg agcaacgtga caaacagaac gtccgcaccc
    10321 gacagccatc atccgtacgc aggagcaaat ttcgcaatgc aacacgagca tctatctccc
    10381 atcactccgt agagcgccga tgagagtcgc aaacacccgc aacctgcacg gccatgtcac
    10441 ccaggaactg ggggtgcgga tcgtcagcgg ccagctccag cccggggagg cattgccacg
    10501 tgaagaagcg cttgccgagc aaatggcggt gagccgcacg gccctgcgcg aagcaatgcg
    10561 tgtgctgtcc gccaagggcc tggtcgaggc acgccccaag gtcggtacgc gggtgcgcga
    10621 agaacgctac tggcatcagc ttgatgccga cgtgttggcc tggcgctgcg cctcgatgcc
    10681 cccggcggat ttcgtgcaga agctggcgca gatgcgcgaa atcttcgagc ccgccgcagc
    10741 cgccctggcc gcgcgccgct gcgatgcctc acagctgaag cagatcgagc tggcataccg
    10801 tgccatggaa gcggcccaag ataccgatga gtgggccgaa gcggatctgc agttccac
//
