LOCUS       NZ_RYYV01000001        10846 bp    DNA     linear   CON 06-MAY-2024
DEFINITION  Dyella choica strain 4 M-K27 scaffold1, whole genome shotgun
            sequence.
ACCESSION   NZ_RYYV01000001
VERSION     NZ_RYYV01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Dyella choica
  ORGANISM  Dyella choica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae; Dyella.
REFERENCE   1  (bases 1 to 567299)
  AUTHORS   Qiu,L.-H. and Gao,Z.-H.
  TITLE     Dyella dinghuensis sp. nov. DHOA06 and Dyella choica sp. nov.
            4M-K27, isolated from forest soil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 567299)
  AUTHORS   Qiu,L.-H. and Gao,Z.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) School of Life Sciences, Sun Yat-sen
            University, No. 135, Xinggang West Road, Guangzhou, Guangdong
            510275, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2018
            Assembly Method                   :: Velvet v. 1.2.10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 145.0x
            Sequencing Technology             :: Illumina Hiseq 4000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003977665.1-RS_2024_05_06
            Annotation Date                   :: 05/06/2024 01:53:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,733
            CDSs (total)                      :: 4,678
            Genes (coding)                    :: 4,648
            CDSs (with protein)               :: 4,648
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 9 of 30
            Pseudo Genes (incomplete)         :: 25 of 30
            Pseudo Genes (internal stop)      :: 5 of 30
            Pseudo Genes (multiple problems)  :: 9 of 30
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:37:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 377966
            Orig. end                         :: 388812
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            RYYV01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10846
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10846
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10846
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5846](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5846)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(177..190)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 20.71"
                     /tool="antismash"
     gene            732..1127
                     /locus_tag="EKH80_RS01810"
                     /old_locus_tag="EKH80_01810"
     CDS             732..1127
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /locus_tag="EKH80_RS01810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH80_01810"
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="WP_196240318.1"
                     /transl_table=11
                     /translation="MKTSQKPTAFGRRHQNRHYAIRLMAAIAVSAFGAIAIHSAYAQST
                     SSSIFGRAPADSTITVHSDNGGARHGTANSKGRYTLTSLLPGTYVVSLERNGQTVAKVQ
                     GVPLFASRSSQVDFVCDNDQCTGTFGR"
     gene            complement(1272..2921)
                     /locus_tag="EKH80_RS01815"
                     /old_locus_tag="EKH80_01815"
     CDS             complement(1272..2921)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EKH80_RS01815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="EKH80_01815"
                     /product="hypothetical protein"
                     /protein_id="WP_126683005.1"
                     /transl_table=11
                     /translation="MRTEFENRNKAKFPSVSTWVLRLMCVVCIVYGSAHANEVWLSPLD
                     PVARAAHNWNASEDYMDLFRADSEWKTVAANLRVFKIGPGFVQQGRDEDLQRIFSELKR
                     RNIKLALEIGMATRSEHCQERTEAYGEPGLVEGLLQRVKRLGGEPSYIAMDEPLYYGHE
                     YQGGDACRLSVRQVAADVAPNVKLAKRIFPNVKIGDIEVVYASKAFLQATEEWVDAYRE
                     AVGFNLEFLHADVSWSPAGMANLPLLSSFVRKSRRISFGIIYNGGNTDGQTDQSWARAA
                     ADHFTYIESGLGVIPDQAVFQTWVALPSHNLPEATAGTLTNLVLEYLRPRSVMTLMRNG
                     TTLSGRLSDSAGAAISGAPISIADIGAMTGETMPTRNLSSTAFKNARAALFAVRANKEL
                     QCTCSGPVTANIGKVTYYENAQAGGGRTNVHEIPGGKIAVDASGTVYNNSAKFPVTPGA
                     AFTLEVPYEARINVEHSAYVAVLFVDDAGHEVGRRFLYLESIKTPIGTVRTRSDGSFSF
                     GINQTVLASGPQMFSADFPGDDRLRSATAMIQ"
     gene            3163..3414
                     /locus_tag="EKH80_RS01820"
                     /old_locus_tag="EKH80_01820"
     CDS             3163..3414
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EKH80_RS01820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="EKH80_01820"
                     /product="hypothetical protein"
                     /protein_id="WP_230473357.1"
                     /transl_table=11
                     /translation="MLVAGGRLTGYAWSEEVLAGIAKDVPYNAEQAMKDPGALYEKALL
                     PFVSNVVVDGRLVTGQNPASAKATAMQVASMLRSPFDA"
     gene            3555..3977
                     /locus_tag="EKH80_RS01825"
                     /old_locus_tag="EKH80_01825"
     CDS             3555..3977
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EKH80_RS01825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="EKH80_01825"
                     /product="hypothetical protein"
                     /protein_id="WP_126683006.1"
                     /transl_table=11
                     /translation="MYLGAVVGDMFCIGGSIAEVFGGELAGELINCAGILIAAPFQVTG
                     GAIDSIKEESELKNQAKEYLKQANELGNAETRDKPDGLHRIRSLGETGGLDDKTIDALV
                     DNAGVFSSSPDKAEEYLRELSKLPQRSRGSFKLGML"
     gene            complement(4249..5001)
                     /locus_tag="EKH80_RS01830"
                     /old_locus_tag="EKH80_01830"
     CDS             complement(4249..5001)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /locus_tag="EKH80_RS01830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH80_01830"
                     /product="DNA-binding domain-containing protein"
                     /protein_id="WP_230473358.1"
                     /transl_table=11
                     /translation="MLADMQRDFHHWLVNTGDENRLQRRLVSPRGLAAYQNNYRSQLVN
                     VLKASYPQLLSRMGEGAFLQTAIRHIDLHPPSSWTLDDYGADFRQTLRVIYPDNPDLDE
                     LAWIEWTLSECFVAPDATTLTIQALGDIDWDHARLRLTPSFRQHVATTNAVDVWSSLHH
                     QTEAIEAEMLDTPAGVIVWRRGFSSQLRQVDAIERAALLSLLNDGDFNAMCDVVVEHLG
                     EAEGVTRAGSLLAAWMASDIVASVEIHG"
     gene            complement(5001..5846)
                     /locus_tag="EKH80_RS01835"
                     /old_locus_tag="EKH80_01835"
     CDS             complement(5001..5846)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465159.1"
                     /locus_tag="EKH80_RS01835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH80_01835"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_126683008.1"
                     /sec_met_domain="DUF692 (E-value: 4.5e-103, bitscore:
                     339.1, seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTSLPTFAGFGLGLRKEHYHDFLETEVPVDFVEVISENFMVDGG
                     QPLDILRRVRERYPVALHGVSMSIGSADGLKPAYLRRLRSVVDRLDPLFVSDHLSWSRF
                     EQFNSHDLLPLPFTEETLAIVCRNIHAAQEAVGRPMLFENPSSYVTFTASTLTEWEFLS
                     EMTRRTGCGLLLDVNNLFVSARNHGWDVEAYLSGIPVDRVRQIHLAGHTQGSDMLIDTH
                     DQPVGVDVWALYARAGSVLGPVATMIERDDAIPPLADLLGELDLARNLRQRHLPLQEAA
                     "
     gene            complement(5907..6191)
                     /locus_tag="EKH80_RS01840"
                     /old_locus_tag="EKH80_01840"
     CDS             complement(5907..6191)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EKH80_RS01840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="EKH80_01840"
                     /product="hypothetical protein"
                     /protein_id="WP_126683009.1"
                     /transl_table=11
                     /translation="MSTITTKSRSSFAAAAAVVALAIASLNGAAHAADQSGHKEQPTRC
                     YGANACKGQSLCAMAKHDCKGLNDCKGQGVIVKTPSECAAAGGTLTEPK"
     gene            complement(6250..7005)
                     /locus_tag="EKH80_RS01845"
                     /old_locus_tag="EKH80_01845"
     CDS             complement(6250..7005)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /locus_tag="EKH80_RS01845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH80_01845"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_126683010.1"
                     /sec_met_domain="Abhydrolase_6 (E-value: 2e-12, bitscore:
                     43.2, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKPLSLLLLMLASTAPAFAAGDAPEGVKNIVIVPGAFVDGSGWR
                     VVHDILTKHGYSVNVVQPRIDTLSNDAEAVYRQLRKESGPTLLVGSDYGGAVITVAGNR
                     DKVKGLVYVAAVAPSVGESVSILVNSVPEPSNDIEASWDGFVYVNEKHFGADLGNDLTT
                     NRTNYLAISQVPATVPALGAVTEAAAWKNKPSWAVVATEDHFYSPDLQRSMYQRAGSRI
                     TEIKGSHLVYMSQPEQVAQVIENAAKSLK"
     gene            complement(7045..7488)
                     /locus_tag="EKH80_RS01850"
                     /old_locus_tag="EKH80_01850"
     CDS             complement(7045..7488)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704060.1"
                     /locus_tag="EKH80_RS01850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH80_01850"
                     /product="DoxX family protein"
                     /protein_id="WP_126683011.1"
                     /transl_table=11
                     /translation="MSAWPAPQRFVPEAMLLLMARLGIAAVFFQSGRTKVEGLLTIKES
                     TYQLFASEYALPLIPSDIAARLSTGAEHLFPVLLVLGLCTRLSALALLGMTTVIEIFVY
                     PDAWPTHLSWAALLLPLVVRGGGAWSMDHIRRNRSLQRSPSVA"
     gene            complement(7485..7760)
                     /locus_tag="EKH80_RS01855"
                     /old_locus_tag="EKH80_01855"
     CDS             complement(7485..7760)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433792.1"
                     /locus_tag="EKH80_RS01855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH80_01855"
                     /product="DUF2282 domain-containing protein"
                     /protein_id="WP_126683012.1"
                     /transl_table=11
                     /translation="MKSTRAALAATFALSLACFAVAGHAENAKKPMEKCYGVALAGKND
                     CAAGPGTTCAGTAKVDYQGNAWILVDKGTCTTFKTPKGYGSLEAKP"
     gene            8156..8458
                     /locus_tag="EKH80_RS01860"
                     /old_locus_tag="EKH80_01860"
     CDS             8156..8458
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /locus_tag="EKH80_RS01860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH80_01860"
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="WP_126683013.1"
                     /transl_table=11
                     /translation="MTHYTYIWEFMVKAGKEAEFVEHYSPDGTWAKLFKQAPGYVETLL
                     LKDRKDGQRYFTVDRWRSEAAYQAFRSRFADSYAALDRVCEDLTTSEKPMGCFDE"
     gene            8451..9071
                     /locus_tag="EKH80_RS01865"
                     /old_locus_tag="EKH80_01865"
     CDS             8451..9071
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GST_N_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1193: glutathione S-transferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /locus_tag="EKH80_RS01865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH80_01865"
                     /product="glutathione S-transferase family protein"
                     /protein_id="WP_230473359.1"
                     /sec_met_domain="GST_N_3 (E-value: 2.5e-19, bitscore: 64.6,
                     seeds: 52, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNDRPLQVYFSPYSPFVRKVLVCAAELGIAIERLPCAAHPVTRDQ
                     AIVALNPLGKVPTLLNSDGEALYDSRVICEYLDATSANPHLFPIQSPARWQSLREQALA
                     DGLLEAAVLMRYEQVVRPEELRYQPWYEGQHAKVISSLDQFEAWVPDFGERVDIGTISV
                     ACALGYLDLRFPGEGWRSRENLAAWYASFSERPSMSGTRHPQA"
     misc_feature    complement(9110..9123)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.59"
                     /tool="antismash"
     gene            9117..9485
                     /locus_tag="EKH80_RS01870"
                     /old_locus_tag="EKH80_01870"
     CDS             9117..9485
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214649.1"
                     /locus_tag="EKH80_RS01870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH80_01870"
                     /product="VOC family protein"
                     /protein_id="WP_126683014.1"
                     /transl_table=11
                     /translation="MFDHVVFGVSDYAASKAFFLKALEPLGIEVVHERPDGIELSSDGK
                     VSLCLLQTEEKPAHLHLAFVAESRQQVEAFHRAALAAGGKDHGAPGLRPHYHANYYAAF
                     VIDPDGHNIEVVCHRPEA"
     gene            9657..10448
                     /locus_tag="EKH80_RS01875"
                     /old_locus_tag="EKH80_01875"
     CDS             9657..10448
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466834.1"
                     /locus_tag="EKH80_RS01875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH80_01875"
                     /product="hypothetical protein"
                     /protein_id="WP_230473360.1"
                     /transl_table=11
                     /translation="MMKNAFPIKSALALCCLGGVIEAGHSMPADPAVSGISAPSSFVIG
                     PEAGDSHSHAAFPHVPKAIIKVSSRVRVTHAVGQGLNFFAIQVNFPNRTWAHGGPQRVS
                     GKYQMNWGGLVSRGGGATDYREENPASDLQLMQNAGDAERSAPYTWVEGKDYELTIERG
                     NQVTLPPGQYIFIGSGPTVSVSNARNMWEWKFTLRPADGHGPVQVSTLYDAADRISSFY
                     IWNECGYGSCGKRQSARWSMPVYSTLDAPGKSVPAQVLTRF"
ORIGIN
        1 agcacaaaac gaaaagctca aacaagagat ggacgcgcta cgcgcacagc agcagaagca
       61 gctgaccatg gatgaagaag cgcggcctac taaagaagcc ttggtgaagg aaattgtcat
      121 ccttcagaag caagcggatg ttcaagctgc tcggttggca tcattcgagc aacggcttga
      181 tcgcaatcaa cacctccaaa gtgccagcta tgccagtgcc gataccgaac attaagcagg
      241 aacattcgct ggagcattag gacagccccc tggctgacgg ttcatggggt aacccgggca
      301 gtcgggtgta ccgtggccac cgcattgcta gccaggcttt tacccccggg ggcttggcgg
      361 cccgccatgc gggccgccat agccaaaagc acttttgctt acctgccgcc gtgtacgtca
      421 acgggaacca tggcagaaag aatgcatgac ggggcttggg ttcgtggcgt aaaaagcatt
      481 acgaccttca catgccccgc ataagccttc ggggttaatt taaacctacc ttcgtcatgg
      541 atcatccaac ggaccgattc aggcgatttg gtgtgaacca aggcgtcaca ggaggagacg
      601 ctactcccaa caaggaagaa aagtatcttg gcagccaccc cactgccgcg atgccattga
      661 catcgattga aagccgacga ccgcgcgagc ggttgaacgg tttgtcacac cccaaaccca
      721 cgagcatccc catgaaaacc agtcaaaaac ctacagcctt tgggcgccgg catcaaaatc
      781 gccactacgc gatcaggctc atggcggcga tagccgtgag cgcattcggc gccatcgcaa
      841 tccattccgc atacgcgcag tcaaccagct ccagcatttt tggcagggca cccgcggaca
      901 gcaccatcac cgtgcacagc gacaacggcg gcgcccgcca cggaaccgcc aacagcaagg
      961 gtcggtacac cctcacttcg ctgctacccg gtacttacgt cgtctcgctg gaaaggaacg
     1021 gccagacagt cgccaaggta cagggtgttc cgctctttgc gagcaggtca tcccaagtcg
     1081 attttgtttg cgacaatgac cagtgcacgg gaacgtttgg tcgctgatca cgcggcccct
     1141 ggtgtcatgc cctagggcat gacaccaaag atccgggcgc ccaccacggg ctccctacag
     1201 cgaagctgat ctgcctggtg ccagtacacg acactccttg attacctttc tggcctggtt
     1261 ggcgccggat gtcactggat catcgccgtc gcgctgcgaa ggcgatcatc gccagggaaa
     1321 tccgcactga acatctgcgg gccggaagcg agcacggttt ggttgatgcc aaaggaaaaa
     1381 gaaccgtccg agcgggttct caccgtgcct atcggcgtct tgatcgattc gaggtagagg
     1441 aatcgccgtc cgacctcatg ccctgcgtcg tcgacaaaca acacggcgac gtaagcggag
     1501 tgttcaacgt tgatgcgcgc ctcgtacgga acttccaacg taaacgccgc accaggcgtt
     1561 acgggaaatt tcgcggaatt gttgtacacg gtaccgctcg catccacggc aatcttgccg
     1621 ccaggaattt catgaacatt cgtcctgcca cctccggctt gagcattctc atagtaggtg
     1681 accttcccaa tgtttgcggt gacggggccg gaacaagtgc actggagctc cttgttcgcc
     1741 cgcacggcga acaaggccgc gcgcgcattc ttaaaggccg tgctggatag gtttctggtg
     1801 ggcatcgttt ctcccgtcat cgcaccgatg tcggcgattg agatgggagc gccgctgatc
     1861 gctgcacccg ccgaatcgct caggcgaccg gaaagggtcg tcccgttacg catgagtgtc
     1921 atcacgctac gcggtcgcag atactcaagc accagattcg tgagcgtacc tgctgttgcc
     1981 tctggcagat tgtgcgatgg aagcgccacc caagtctgaa aaaccgcctg atccgggatg
     2041 acgccaaggc cggactcgat gtaggtgaaa tggtccgccg ctgcacgagc ccaggattga
     2101 tccgtttgtc cgtcggtgtt gccgccgttg tagataatgc caaaacttat ccggcgtgac
     2161 tttctgacaa aagaagacag cagcggcagg ttggccattc ccgccggcga ccagctcaca
     2221 tccgcgtgca ggaattccag attgaatcca actgcttcgc ggtacgcatc cacccattcc
     2281 tcggtagcct ggagaaacgc cttcgaagca tagacgactt cgatatcccc gatcttgacg
     2341 ttgggaaaga tgcgcttggc caacttcaca ttcggggcca cgtccgcagc tacttgccgg
     2401 acggaaagcc tgcaggcgtc gccgccttga tattcatggc catagtagag cggctcgtcc
     2461 atggcgatat aggaaggttc tccgcccagg cgtttgactc gctgcagcaa tccctccacc
     2521 aaacccggct ctccataggc ctcggtgcgc tcctgacaat gctccgaccg cgtcgccatc
     2581 ccgatctcca gcgccagttt gatgttgcga cgcttgagct cggagaaaat ccgttgcaga
     2641 tcctcgtccc tgccctgctg cacgaatccc ggccctattt tgaagacgcg caggttcgcc
     2701 gccaccgttt tccactcgga atccgcgcgg aacaaatcca tgtaatcctc gctggcgttc
     2761 cagttgtgag ccgcgcgtgc gaccgggtcc agcgggctga gccaaacttc attggcatgg
     2821 gcgctaccgt agacgatgca aacgacgcac atcagccgaa gtacccaagt gctgacggat
     2881 ggaaattttg ccttgtttcg attttcgaat tcggtccgca tgatcatcct ttgcgatggc
     2941 ttcaaccggg gttccacgag caccgtgcat ccattgactg aattcgcagt cgaatgtgga
     3001 catcccgcct atgctttcag cacctgcctt ggctgtcaaa gggccagttc aggtgcgtac
     3061 ctgttgaacc caagcacgcg ttttggaagc ccaaccgggt tcacatccgc cggaatgcca
     3121 ggaacgaggt ggccgctgcg gttcgagtta tcggatggca gcttgctggt ggccggtggt
     3181 cggctgaccg gctacgcctg gtctgaagag gtgttggccg gcattgccaa agatgtgccc
     3241 tacaacgccg agcaagccat gaaagacccc ggcgcgctgt acgaaaaagc cttgcttcca
     3301 ttcgtctcaa acgtagtggt ggatggtcgg ctcgtcacgg gtcaaaaccc ggcatcggcc
     3361 aaagccaccg ccatgcaggt cgcttccatg cttcgatcac cctttgacgc gtaaagaaag
     3421 aaccgctttc gcaaaaaaaa ccggtcaggc gcgttattgg ttttcaggga aatccctagc
     3481 tcgggatagc cccagcttgt gggtgcaaag cgcccggcac ctcacaggat ctcagcaagt
     3541 ccttaaatga cccggtgtat ctaggcgccg tcgtcggcga catgttttgc atagggggaa
     3601 gtattgccga agtctttggc ggtgagcttg ccggcgagct catcaactgc gcggggatac
     3661 tgatagccgc accgttccag gtcacgggtg gcgctatcga cagcatcaag gaagagagtg
     3721 aactgaaaaa tcaagcaaag gaatatctaa agcaagcgaa tgagctcggt aacgccgaga
     3781 cacgggataa accggacggt ttgcatagaa tccgatcgct gggtgaaacc ggcggactgg
     3841 atgacaagac catcgacgcg ctggtcgata atgccggcgt cttttcatcg tcacccgaca
     3901 aagccgagga gtacttgcgc gagctgagca agttgcccca gcgaagccgc ggctctttta
     3961 agctagggat gctctgatct attccacgtg cccggccttc tggacgtcac cccgtcattc
     4021 aaatttaaga tgtgggggcc gaactgtata cgcgtctggt ggagcgtagg ctgggatggc
     4081 aatcccagca tcgcgcggct gtaggcatgg caatgctggg tttctcaacc cagcctacgc
     4141 tccgccagac gcgcatacag ggcggtgacg ggtacgtgga atagatcaga gcatccctag
     4201 ggggccggaa cttccgccta gctcttcaac gcgagcgatg cgcctagatc atccatggat
     4261 ctccacgctc gccacgatgt cggaagccat ccaggcggcc agaagactgc cggcgcgagt
     4321 aacgccctct gcttcgccaa ggtgctcaac gacgacatca cacatggcgt tgaagtctcc
     4381 atcattcagc agcgatagga gcgccgcgcg ttcaatcgca tcgacctgtc tgagctgtga
     4441 tgaaaacccg cgacgccaga caattacgcc ggctggcgta tcgagcattt cggcctcgat
     4501 cgcctcagtc tgatggtgca atgaggacca gacgtcaacg gcattggtgg ttgcgacgtg
     4561 ctgacgaaag cttggcgtga gacgaaggcg tgcgtggtcc cagtcaatgt cacccaatgc
     4621 ctggatggtc agggtcgttg cgtctggcgc cacgaagcac tccgaaagtg tccactcgat
     4681 ccacgcaagt tcgtccagat ccgggttgtc cgggtatatc acacgcaagg tctggcgaaa
     4741 gtccgcgcca tagtcgtcca gggtccacga cgacggcgga tgaaggtcga tgtgacggat
     4801 ggctgtctgt agaaaggcgc cctcacccat tcggctgaga agttgcggat agctcgcctt
     4861 gaggacattc accagctggg aacgatagtt gttctgataa gctgcaagcc cgcgcgggga
     4921 aacaagtcgg cgttggagac gattttcgtc acccgtgttg accagccaat ggtgaaaatc
     4981 ccgctgcatg tcggccaaca tcacgctgcc tcctgcaagg gaagatggcg ttggcgcaaa
     5041 ttgcgcgcaa ggtcgagttc gcccaacaga tcggcaagcg gcggaatggc gtcatcgcgt
     5101 tcgatcatgg tggcgacagg gcctagcacg gacccagctc tcgcatacag cgcccacacg
     5161 tccacgccta cgggttggtc atgggtgtcg atcagcatgt ccgagccctg ggtgtggccg
     5221 gccaggtgga tctggcgcac gcggtcaacc ggtatgccgc tcaggtaggc ctccacgtcc
     5281 cagccatggt tccttgcgct gacgaatagg ttgttgacgt cgagcaagag gccgcagccc
     5341 gtacgtcggg tcatttcgct gaggaactcc cactccgtca aggtggaagc cgtgaaggtg
     5401 acgtaactcg acgggttctc gaacagcatc ggcctgccga cggcctcctg cgcggcgtga
     5461 atgttccggc agacgatggc cagcgtttcc tccgtaaagg gaagaggaag caggtcgtgc
     5521 gaattgaatt gctcgaagcg agaccagctc aaatggtccg atacgaacaa ggggtcaagc
     5581 cgatccacca cagaacgcag gcggcgcaga tacgccggct tcaagccgtc cgccgagcca
     5641 atggacatcg atacgccgtg gagcgcgacg ggatagcgct cgcgtacgcg gcgcaggata
     5701 tccaacggct gcccgccatc gaccatgaag ttctcggaga tcacttccac gaaatcgacc
     5761 ggcacctcgg tttcaagaaa gtcgtggtaa tgctctttgc gcaagccgag accgaagccg
     5821 gcaaaggtgg gaagcgatgt ggacatggcg atgctcctgg gcaaggaaag gcgctggacc
     5881 cgctcgcagc gggtccagcc aggcgatcac ttcggttcgg tcagcgtgcc gccggctgcg
     5941 gcacactcgc tcggggtctt tacgatgacg ccctggccct tgcagtcgtt gaggcccttg
     6001 cagtcgtgct tggccatggc gcaaaggctt tggcccttgc aggcgttagc tccgtaacag
     6061 cgggtcggct gttctttgtg gcccgactga tcggcggcgt gcgcagcacc gttgagtgat
     6121 gcgatagcga gtgcgacgac ggcggccgcc gcagcgaaac tgctgcggga cttggtggtg
     6181 atggtgctca tgagtcgttt tcctgttggg taagggttta ggtcaaacca ttgggggtat
     6241 gacgcggcgc tacttcaagc tcttcgccgc gttctcgatc acttgcgcaa cctgttccgg
     6301 ctgcgacatg tagacgaggt ggctgccctt gatctcggtg atcctggaac cggctcgctg
     6361 atacatcgag cgctgcagat cggggctgta gaagtgatct tcggtcgcaa ccaccgccca
     6421 gctgggcttg ttcttccagg cagccgcttc ggtaacggcg cccagcgcag gaaccgttgc
     6481 gggcacctgg gaaatggcca ggtagttggt gcggttggta gtcagatcgt tgcctaggtc
     6541 ggcgccgaag tgcttctcgt tgacgtaaac aaatccgtcc caggaggctt cgatgtcgtt
     6601 gctgggctcc ggcacggagt tgactagtat gctcacgctt tcacccacgc tcggcgctac
     6661 cgcggccacg tagaccaggc ctttcacctt gtcgcgattg ccggctaccg tgatcaccgc
     6721 gccgccgtag tcactgccta ccagcaaggt cgggccactc tccttgcgca actggcgata
     6781 gacggcttcg gcgtcattac tcagcgtgtc gatgcgcggt tgcacgacat ttacgctgta
     6841 accatgcttg gtaaggatgt cgtggacgac gcgccagccc gaaccgtcga cgaaggcgcc
     6901 ggggacgatg acgatgttct tgacgccttc gggggcgtcg ccagcagcga acgcgggtgc
     6961 ggtcgacgcc agcatgagca acaacaaact caatggcttt cgcatgatgt ttcctgagga
     7021 agtgaaattg atgggaatgg ggagtcacgc aacggaaggc gaccgttgca gcgatcggtt
     7081 gcgacggatg tgatccatcg accacgcgcc gccgccgcgg accacgagcg gaagcaacag
     7141 agcggcccag ctaaggtgcg ttggccatgc gtccgggtag acgaagatct cgatgaccgt
     7201 ggtcatgccc agcaaggcga gcgcggacag ccgcgtgcac aagcccagca ccagcagcac
     7261 gggaaacagg tgctccgcgc ctgtggacaa acgtgcggcg atgtccgagg gaatcagtgg
     7321 cagcgcgtac tcgctggcaa acagctgata ggtggactct ttgatcgtca ggaggccttc
     7381 cactttcgta cggcccgact ggaagaacac cgcagcgatg cccagccgtg ccatcagcaa
     7441 tagcatggcc tccggaacga aacgttgcgg cgcgggccat gcgctcatgg cttggcctca
     7501 agcgaaccgt accccttggg ggtcttgaag gtcgtacagg tgcccttgtc gaccaggatc
     7561 caggcattgc cctggtagtc gactttggca gtgcccgcac acgtcgtacc agggccggcg
     7621 gcgcaatcgt tcttgccggc gagcgccacg ccgtaacatt tttccatcgg cttcttggcg
     7681 ttctcggcat gaccggccac ggcgaagcag gccaggctga gggcgaatgt ggcggccagt
     7741 gccgcgcgag tgcttttcat gatgatttcc ttgggaaggt ggggaatttt tcggctcagc
     7801 gcagctcaag ccacagcgat acgctgttac ggttagcttt agcgcggatc ttgtccgcgc
     7861 atcgggtgtt gggcatcggg taactccggt atgactggaa atgccggtcg cgacccactg
     7921 aatcaggtct tacgcgagga ggctgggtgg gcagaaaact accccctacc cgattcattc
     7981 agtagccggt aaattcgaga ggcgcccttt cacagggtga gaggctcagg acgaaaaaga
     8041 ggcccgcttt cgaccaaagc ggaccgcttc gacggacgat gtacgctgac gcgcttgcgt
     8101 gtaaaagatg agcctggctg cgtatcgtca ttgacccgag cggggcatcg caccaatgac
     8161 ccactacacc tacatttggg aattcatggt caaagcagga aaagaggctg agttcgttga
     8221 gcactacagc ccggatggga cgtgggcaaa gctattcaag caagctcctg gatacgtcga
     8281 aacattactg ctcaaggaca ggaaggatgg acagcgctat ttcactgtgg accgttggcg
     8341 cagcgaagcg gcctatcaag ccttcagatc ccggttcgct gactcgtatg ccgctcttga
     8401 ccgggtctgt gaagacctga ccacttcgga gaagccaatg ggctgtttcg atgaatgacc
     8461 gacccttgca agtctacttc tcgccctact cgcccttcgt acgcaaggtg ctggtttgcg
     8521 ctgccgagct cggtattgca atcgaacgtc tgccctgcgc agcgcatccg gtgacgcgcg
     8581 atcaagccat cgttgcgctc aatcccctcg gcaaggtgcc aacgctcttg aattccgatg
     8641 gcgaggcgct ctatgacagc cgggtgatct gcgagtacct cgatgcgaca agtgcgaatc
     8701 cccacctgtt tccgatccaa tcccccgcgc gttggcaaag cctgcgggaa caggcgcttg
     8761 cagatgggct cctcgaagcg gctgtgctga tgcgctacga acaggtcgtt cgtcccgagg
     8821 agttgcgcta ccaaccatgg tatgaagggc aacacgcgaa ggtgatctcg tccctggacc
     8881 agttcgaggc atgggtgccg gacttcggcg aacgcgttga catcggtacg atttcggttg
     8941 cctgtgcgct cggttacctc gacctgcgtt ttcccggcga aggatggcgt tcgcgcgaga
     9001 acctggcggc gtggtatgca agtttcagcg aacgaccctc catgtcagga actcgccatc
     9061 cccaagcctg ataccattga gcaggacacg cagaccccca cacttcggga gaactcatgt
     9121 ttgaccacgt cgtcttcgga gtcagcgatt atgcagcgag caaagcgttt ttcctcaagg
     9181 cactcgaacc gctcggcata gaggtcgtcc acgagaggcc ggacggcatc gagctcagtt
     9241 cggacggcaa ggtttcattg tgcctgctgc aaaccgagga gaagccggcg catctccacc
     9301 tggcgttcgt cgccgagagc cgccagcaag tcgaagcctt tcatcgcgca gctctggctg
     9361 cgggcggcaa ggaccatggc gcgcccggtc tgcgcccgca ttaccacgcg aactactatg
     9421 cagctttcgt gattgatccg gacgggcaca acatcgaagt ggtttgccac agacccgagg
     9481 cctgatcctt ccatctatag gatgttcaga gcgggaatcg cacgcttgcg ctcgccctcc
     9541 ttccaaacgt tatcgacgtc atcggggagt acgggaattg gtcatggtgt gattgagcag
     9601 ggccatcttg gcatggatgg ctatgagatc gaactgctgt acgggaacaa ggattgatga
     9661 tgaagaacgc ctttccaatc aagtctgctc tggcgctgtg ctgcctgggc ggtgtcatcg
     9721 aggcggggca ttcgatgccg gctgatccag ctgtgtccgg gatatcggca ccgtcaagct
     9781 tcgtgattgg gccggaggcc ggcgattctc actcgcacgc ggccttcccc cacgtgccca
     9841 aggccatcat caaagtgtca tcgcgtgtgc gcgtaaccca tgccgtgggc caaggtctca
     9901 acttttttgc catccaggtc aattttccca atcgaacctg ggcccatggc ggccctcagc
     9961 gggtgagcgg caagtatcaa atgaactggg gtggcctggt gtcccgcggg ggcggcgcta
    10021 cggattatcg cgaggaaaac cccgcttcgg atctgcagct gatgcagaac gcaggtgatg
    10081 ctgagcgcag tgcaccgtac acctgggtgg agggaaaaga ctacgagctg acgatcgagc
    10141 gtggcaatca ggtcacgctt cccccgggac aatacatctt catcggatct ggccccaccg
    10201 tttccgtaag caacgcgcgc aacatgtggg aatggaagtt cacgctgagg ccggcggacg
    10261 ggcatggccc ggtccaagtg tccaccttgt atgacgcggc cgatcggatc tccagtttct
    10321 acatctggaa cgagtgcggt tacggctcat gcggtaagag acagagcgcc cgctggtcca
    10381 tgcctgtcta ttcgacgctc gacgcaccag gcaagagcgt gccagcacaa gtgctgacac
    10441 gtttttgagc cgccaatcac cttcacgtcc ataccttgcg aggatcggcg tcagggaagt
    10501 gcgtaggctg ggatggcagt cccagcatcg ggatgtcgcg tggacagctg ggattatcat
    10561 cccagcctgc gtgcttcatc tgtgttgtat cgtagatgtg cttgcactac gtctcgggag
    10621 gaggacggta tgagctttca aaggcgccaa tggcttcaat ccttgtcata cgctttcggg
    10681 gccagcttca ttctgcccag gaacgtattc gccgtgacgg cacgaaaccc cgcgcaaccg
    10741 gcaactgctg ctacgccagg gcaggcaaat gccaaacatg agtcagcggc agcggaaagg
    10801 gagaaagtga ccggaatcgg tggactgttc tttcgagccc atgatc
//
