LOCUS       NZ_CP034587            11011 bp    DNA     linear   CON 19-MAY-2024
DEFINITION  Streptomyces luteoverticillatus strain CGMCC 15060 chromosome,
            complete genome.
ACCESSION   NZ_CP034587
VERSION     NZ_CP034587.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces luteoverticillatus
  ORGANISM  Streptomyces luteoverticillatus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7367863)
  AUTHORS   Feng,Z., Chen,G., Zhang,J., Zhu,H., Yu,X., Zhang,W. and Zhang,X.
  TITLE     The whole draft genome of Streptomyce luteoverticillatus CGMCC 15060
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7367863)
  AUTHORS   Feng,Z., Chen,G., Zhang,J., Zhu,H., Yu,X., Zhang,W. and Zhang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2018) School of Life Sciences, Ludong University,
            186 Hongqi Road, Zhifu Distric, Yantai, Shandong 264025, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 247.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003970715.1-RS_2024_05_19
            Annotation Date                   :: 05/19/2024 04:34:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,575
            CDSs (total)                      :: 6,474
            Genes (coding)                    :: 6,276
            CDSs (with protein)               :: 6,276
            Genes (RNA)                       :: 101
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 198
            CDSs (without protein)            :: 198
            Pseudo Genes (ambiguous residues) :: 0 of 198
            Pseudo Genes (frameshifted)       :: 104 of 198
            Pseudo Genes (incomplete)         :: 118 of 198
            Pseudo Genes (internal stop)      :: 12 of 198
            Pseudo Genes (multiple problems)  :: 30 of 198
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:02:22
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 892161
            Orig. end                         :: 903172
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP034587.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11011
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="11"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11011
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11011
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:6011](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6011)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(556..1536)
                     /gene="sbnA"
                     /locus_tag="EKH77_RS03655"
                     /old_locus_tag="EKH77_03655"
     CDS             complement(556..1536)
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="sbnA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1081: cysteine synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017236958.1"
                     /locus_tag="EKH77_RS03655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH77_03655"
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="WP_126912994.1"
                     /sec_met_domain="PALP (E-value: 1.3e-50, bitscore: 171.1,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIYQRACDIVTDEVFLELDGFLPGSHLFLKLEGMNPAGSVKLKSA
                     LGMVEDAERNGVLWPGGRIVESSSGSLGIALSVIAAARGYHFTCVTDPNISPQSLALIQ
                     ALGAEVVQVTRRDDNGGYLGTRIARIREMTEADPGLVWLNQYANPANAQAHARRTATSI
                     LKNIGHVDVLLVGAGTTGTLMGCTRHFRTYSPWTRIVAVDTTGSVTFGGPPGPRYIPGL
                     GTSRRPELCRPDRVDEVVLVDEPDAVRMCRRLAREHGLPVGGSTGSVLAALESIADRIP
                     AGSRVVALSPDMGDRYIRTIYDDMWVADTFGTGVLEAPGAQPVAV"
     gene            complement(1533..2948)
                     /locus_tag="EKH77_RS03660"
                     /old_locus_tag="EKH77_03660"
     CDS             complement(1533..2948)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1247: acetylornithine deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018562660.1"
                     /locus_tag="EKH77_RS03660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH77_03660"
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="WP_126912995.1"
                     /transl_table=11
                     /translation="MSAPPLAANVRALMPRAREDLAELVALRTVVGNAPPDHFPRAARR
                     IAALLTDAGLPEIRLLSADDGTETVYARRPGPPGAPSVVLYAHYDVVSAQDETAWRTPP
                     FELTERDGRWYGRGSADCKGNVVMHLTALRALRDALPHDPYPDLDIVVLVDGSEEPGDA
                     SLEKLLRAHPGLIAPDVVVIADTGNVAPGIPTVTTSLRGMAVVEVTLTTLSRTLHSGQF
                     GGPTPDAMTALIRLLATLHDSDGTPSVPVPDTGTVAGRGDSGRGAEYAEADFRADAGVL
                     DGVELIGTGTVADRLWTLPSVTVLAVQSPPLDDAVPALLPTAKALVSVRTPPGADVVRV
                     HEELAGHLRARVPWGAHLEVRRVTSGPSFLADTGGTAHAVMRDAMRDAFGVAPREVGAG
                     GSIAVCSAFRSLHPDAEIILCGVEDPPSNIHGTDESVSPGEIASLAHAEALFLRRLAAG
                     AVRTPSTGPGEDR"
     gene            complement(2978..3913)
                     /locus_tag="EKH77_RS03665"
                     /old_locus_tag="EKH77_03665"
     CDS             complement(2978..3913)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /locus_tag="EKH77_RS03665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH77_03665"
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="WP_164737732.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 6.1e-60, bitscore:
                     200.1, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPSPIVIVSDALPGETLAVLKSTCDVRHLDGRDRKALFAALTDAD
                     ALIVRSGTRVDAEVLDRAPRLRVVGRAGVGLDNVDTITAAARGIAVVNAPGSNTVSVAE
                     LTVGLIVASARHITAAARSLEDGQWRRSEFAGVELAGRTLGIVGYGRVGRQVARRMAAF
                     DMRVLAHDPYADIADGDVHATGLDELLATSDVVTLHLPRTPGTAGLIGARELSLMKPTA
                     YLVNTARGGIVDESALTRALREGRPAGAALDVFATEPPGSSPLLALPNVVALPHLGAST
                     HDAQLRAGREVAGKVLAELAPLLSPSPTAV"
     misc_feature    3964..3977
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 20.13"
                     /tool="antismash"
     misc_feature    complement(3974..3987)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.14"
                     /tool="antismash"
     misc_feature    complement(3975..3988)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.44"
                     /tool="antismash"
     misc_feature    complement(3976..3989)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.85"
                     /tool="antismash"
     misc_feature    3977..3990
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 18.22"
                     /tool="antismash"
     misc_feature    3978..3991
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.06"
                     /tool="antismash"
     gene            complement(4075..5004)
                     /locus_tag="EKH77_RS03670"
                     /old_locus_tag="EKH77_03670"
     CDS             complement(4075..5004)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /locus_tag="EKH77_RS03670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH77_03670"
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_164737733.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 2.5e-13, bitscore:
                     48.3, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNRPRVLVTGGSGFVGGAVVPLLRSSGARVRLLAHRRPLVRGPGV
                     EVVDGDLTDPATLRGICRDVDCVLHLASHVGRDEASGVAVNTDGTRHLVAEAGRAGVGK
                     FVRLGTAAVYGNGPFREAAEGAVEPRPVSPTSRSRLSGERFVLEAGGAVLRPYLVYGTG
                     DRWVMPSLLRMLRALRSGLPGHGSALLSLTEVGSLATSLARMAVTTPPRRAAGVFHACP
                     PRPVSLRELVVATHRHLGFPLVTGGQPLERARAEMVAAGGDGHLLDLFGVDHWFDATRL
                     RTVGDHAPETGLSAGLAAHAAWYREQLT"
     gene            complement(5001..6011)
                     /locus_tag="EKH77_RS03675"
                     /old_locus_tag="EKH77_03675"
     CDS             complement(5001..6011)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="EKH77_RS03675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH77_03675"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_126912997.1"
                     /sec_met_domain="AfsA (E-value: 5.4e-20, bitscore: 69.8,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVDLSSPSVLTACPPPLATERLRPLSFDRTAPRDLVHRRAIGEV
                     LLTDWIRHGPHRFKVGAQWPRGHGFYGPVGHRWHDPLLAAESIRQTSSLVAHAFYELPC
                     DHPTLMTELDFSLSPHALRLDDRPVNVELTIDCTDVTLRGDLLAGMTMNVGLAWGAVRL
                     GHGRMTLNCLRRSVYRRLRGAYAEVPVRLPEQPAPLPPHEVGRAVEADVVLGRPPAGAD
                     TTDPYEGAWPLRVNWAHPTLFDHPTDHVPGMLLMEAARQAAQRLAGPGAILAPAMTNRF
                     HRYVEFDAPCLVRARLAMTGVGTYEVRVTAEQNGAVAYECDLVAHRAPSTSRTRR"
     misc_feature    complement(5943..5945)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(6116..6129)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.14"
                     /tool="antismash"
     misc_feature    complement(6117..6130)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.86"
                     /tool="antismash"
     misc_feature    complement(6118..6131)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.05"
                     /tool="antismash"
     gene            6186..6821
                     /locus_tag="EKH77_RS03680"
                     /old_locus_tag="EKH77_03680"
     CDS             6186..6821
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF041196.1"
                     /locus_tag="EKH77_RS03680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH77_03680"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_126912998.1"
                     /transl_table=11
                     /translation="MAQQERAIETRRRLLIGAAEVFDERGYAAASINEIQERSGITKGA
                     MYFHFASKEEIARGVMAAQTELPVDTRSRSASPMQRAIDLTHELAHQLRTNVLFRASIR
                     LTVEQGTFQANDSDLYDWWLRAFGQELTRARDGGELREELVPEDVAASVVGSFTGIQML
                     SHVMCDRRDLHERLARWWEQILPGIATREALRRALPTGSPELGLTGGT"
     gene            complement(6860..7459)
                     /locus_tag="EKH77_RS03685"
                     /old_locus_tag="EKH77_03685"
     CDS             complement(6860..7459)
                     /EC_number="3.1.3.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1101: phosphoglycerate mutase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020130746.1"
                     /locus_tag="EKH77_RS03685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH77_03685"
                     /product="histidine phosphatase family protein"
                     /protein_id="WP_126912999.1"
                     /transl_table=11
                     /translation="MTVRAMLLAPAPGPALREARFDDDSPLDEAGLGRARAVAAAVPRA
                     SRLLASPSRRCRETAAALGFAPALAAPADLDAGRWRGRTLGELSAAEPGAVATWLADPG
                     AAPHGGESVLALVGRVGAWLDGLGGEGDEANEEGDDGRVLAVTEPVVIRAAVVHALALP
                     PRAFWRLDVRPLTLTVLSGRAGRWNLRCGEELGSAA"
     gene            7641..8759
                     /locus_tag="EKH77_RS03690"
                     /old_locus_tag="EKH77_03690"
     CDS             7641..8759
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1104: putative esterase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942555.1"
                     /locus_tag="EKH77_RS03690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH77_03690"
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="WP_126913000.1"
                     /transl_table=11
                     /translation="MGLTSQTFLLTLVVIAVAVTALVLWLWPRAAKKGPLAFASRLGML
                     VLVQLCVLSVLLVAANNEFGFYSTWKELLGQNSRKQAVRAGSGNGKRPPALTVKGQEPV
                     GLGPKEKAGQVDRIEINGPVTGLSMDGFAYLPPQYFQKGHEKDTFPVIVSITGQPGQSI
                     NLITKAKVPQAASKAVMAGQMKPTIVLMLRPSVVADRDTNCTDVPGGPQALTFFNQDVP
                     VAVNDVYRVDNTRGGWGAVGNSTGGYCALKMAMTNPYRYGAAAGLSTDLFAREDRDTGD
                     LYAGNKQIKNESDLTWRLEHMPPPPVSLLLAASEKGEEKYYAQMQKFAGKAKPPTKVEK
                     LVREEGGHNFVTWREEYPVVFRWLDKELATRR"
     gene            8899..10398
                     /locus_tag="EKH77_RS33615"
                     /old_locus_tag="EKH77_03695"
     CDS             8899..10398
                     /EC_number="2.7.11.1"
                     /GO_function="GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003989212.1"
                     /locus_tag="EKH77_RS33615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EKH77_03695"
                     /product="serine/threonine-protein kinase"
                     /protein_id="WP_240667205.1"
                     /sec_met_domain="Pkinase (E-value: 2.7e-49, bitscore:
                     166.4, seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTERRKPLLAGRYRLMEQLGRGGMGVVWLAMDEVLDREVAAKEV
                     QAPERMNADDVRVLYARLKQEARAAARVSHPNVITVHDVVEQEGRPWIVMELVRGQSLD
                     AVLRQEGVLTPKEAARIGALVLQALRAAHAVGVLHRDVKPANVLMEAGGRVVLTDFGIA
                     LVEGAGTLTRTGDLIGSAEYLAPERATGKRPGIPSDLWSLGTLLYVAVEGVSPFRRGDA
                     LSTLRAVVDDPLAPPRRAGPLTPLLEGLLRKDPARRMGSAEAEQLLDAVADGRLTEQPG
                     YVPTTLDEPVRARTETPSPLPLRETPPPFTPTPTPPPTPTPSRAPTPTPTPTPAPSPMP
                     TPRPWAGPSPVGSPTPPPVPVGLQQGGLATPLVVPAYGDPRHPGYPVGPDPDPDPVPDP
                     GRRKAAWIVAAVTALVLVAGGTAGVIAFRDAGGGTGTPGGGPTASVSQGAGSDQDGQSQ
                     APATGATGDDGSGNPSPTPSPTYEPSPSARTSCGGGWVTSC"
     misc_feature    10997..11011
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: medium, score: 24.07"
                     /tool="antismash"
ORIGIN
        1 cctcccggtc gtgcacgacg aaacggttca cggaccaggc ctgcgcggcg aggaactcgg
       61 cgatgttgcg cgcgatgacc ccggcgccca ccagggctat ccggtccgcg acccggccgc
      121 cggtgagttc ctgggcgccc aggacggcgg acgcggcggt gcgggcggcg ctgatctggg
      181 aggcctccag gcaggcgaag gggtatcccg tctcgtagtc gttgaggagc agggtggcgg
      241 aggcgcgggg gatgttccgc tcgatgttgc gggggtagct ggcgatccac ttgatgccgg
      301 agacaccgtg gtcgccgccg aggtgggcgg gcagggcgat gatgcgggcg tccggcttct
      361 ccgggaagcg caggaaatag ctgttggggt tgaccgagtc accggcgtcg tgtgtgaggt
      421 aggcggagcg gaccgtctcg acgatctcgg gacgggatac gtcgatgagg gtgcgcaggg
      481 cggagccgcc caccacgtgg aagtcgaaca tgcggggttc tcttttctcg tcggtgtgat
      541 cgcgccgggg gccggtcata cggcgacggg ttgcgcgccg ggggcctcga ggacgccggt
      601 accgaaggtg tcggccaccc acatgtcgtc gtagatcgtc ctgatgtagc ggtcgcccat
      661 gtccggggag agcgccacga cgcgcgagcc cgcggggatc cggtcggcga tcgactccag
      721 ggccgccagg acggatccgg tggagccgcc gaccggcagg ccgtgttcgc gggccaggcg
      781 ccggcacatg cggacggcgt cgggctcgtc gacgaggacg acctcgtcca cccggtccgg
      841 ccggcacagt tcggggcggc ggctggtgcc gaggccgggg atgtagcggg ggccgggagg
      901 accgccgaag gtgacggatc cggtggtgtc gaccgcgacg atccgcgtcc acggcgagta
      961 cgtccggaag tgccgggtgc agcccatgag cgtgccggtg gtgcccgcgc cgaccagcag
     1021 gacgtcgacg tgcccgatgt tcttcaggat cgaggtggcg gtccgccggg cgtgcgcctg
     1081 cgcgttggcg gggttggcgt actggttgag ccacaccagg cccgggtcgg cctcggtcat
     1141 ctcccggatg cgggcgatcc gggtgcccag gtatccgccg ttgtcgtccc gccgggtgac
     1201 ctggacgacc tcggctccga gggcctggat gagggccagg ctctgggggg agatgttcgg
     1261 gtcggtcacg caggtgaagt ggtatccgcg ggcggcggcg atgacggaca gggcgatgcc
     1321 gaggctgccg gaggacgact ccacgatccg gccgccgggc cacaggacgc cgttgcgttc
     1381 ggcgtcctcc accatgccga gcgccgactt gagcttcacg gacccggccg ggttcatccc
     1441 ctccagcttc aggaagaggt gactgcccgg caggaagccg tccagttcca gaaacacctc
     1501 gtcggtgacg atgtcgcagg cgcgctggta gatcatcggt cctcccccgg gccggtcgac
     1561 ggagtgcgaa cggcaccggc ggccagccgg cgcaggaaga gggcttcggc gtgggccagc
     1621 gaggcgatct cccccgggct cacgctctcg tcggtgccgt ggatgttcga cggcgggtcc
     1681 tcgacgccgc acaggatgat ctcggcgtcg gggtggagcg accggaaggc ggagcagacg
     1741 gcgatggagc cgccggcccc gacctcgcgg ggcgcgacgc cgaaggcgtc ccgcatggcg
     1801 tcgcgcatca cggcgtgggc ggttccgccg gtgtccgcga ggaaggacgg gccgctggtg
     1861 acgcgccgga cctccaggtg cgcgccccag gggacccggg cccgcagatg gccggcgagc
     1921 tcctcgtgga cgcgcacgac gtccgccccg ggcggggtac ggacgctgac cagcgccttc
     1981 gccgtcggca ggagcgcggg tacggcgtcg tcgaggggcg gtgactggac ggccagcacg
     2041 gtgacggagg gcagggtcca cagccggtcg gcgacggtac cggtgccgat gagttcgacg
     2101 ccgtccagga cgccggcgtc ggcccggaag tccgcctccg cgtactccgc accgcgcccg
     2161 gaatccccgc gccccgctac cgtcccagtg tccggaacgg gcacgctcgg tgtgccgtcg
     2221 ctgtcgtgca aggtggccag gaggcggatc agggcggtca tcgcgtcggg cgtgggcccg
     2281 ccgaactggc ccgagtgcag cgtcctggac agcgtggtga gggtgacctc cacgacggcc
     2341 atgccgcgca gggaggtggt gacggtcggg atgcccgggg cgacgttccc ggtgtccgcg
     2401 atgacgacga cgtccggcgc gatgaggccg gggtgggcgc gcagcagctt ctccaggctc
     2461 gcgtcgccgg gctcctcact gccgtcgacg aggaccacga tgtcgaggtc ggggtagggg
     2521 tcgtggggca gggcgtcccg gagcgcgcgc agcgccgtga gatgcatgac gacgttgccc
     2581 ttgcagtccg cgctgccgcg gccgtaccag cggccgtcgc gttcggtcag ctcgaaggga
     2641 ggtgtccgcc aggcggtctc gtcctgcgcg gagacgacgt cgtagtgggc gtagagcacc
     2701 acggacggcg ctccgggcgg accggggcgg cgggcgtaga cggtctcggt gccgtcgtcg
     2761 gcggaaagca gccggatctc gggcaatccg gcgtcggtga gcagggcggc gatcctgcgg
     2821 gccgcgcggg ggaagtggtc cgggggcgcg ttcccgacga ccgtgcgcag cgcgaccagt
     2881 tcggcgaggt cctcgcgggc tctgggcatc agggcgcgca cgttcgcggc caaggggggt
     2941 gcggacatga tggtctccgg cgtgggggtg ggggcgctca gacggcggtg ggcgagggag
     3001 aaaggagcgg ggccagctcc gcgaggacct tcccggcgac ttcccggccg gcccgcagct
     3061 gggcgtcgtg cgtgctggcg ccgaggtgcg gcagggcgac gacgttcggc agggccaaca
     3121 gcggcgagga gcccgggggt tcggtggcga agacgtccag cgccgcgccg gcgggccggc
     3181 cctcgcgcag cgctcgggtc agggcgctct cgtcgacgat gccgccgcga gcggtgttga
     3241 ccaggtaggc ggtgggcttc atcagggaca gctcccgggc gccgatgagt ccggcggtcc
     3301 cgggggtcct cggcaggtgg agggtgacga cgtcgctcgt ggcgaggagt tcgtcgaggc
     3361 cggtggcgtg gacgtcgccg tcggcgatgt ccgcgtaggg gtcgtgcgcg aggacgcgca
     3421 tgtcgaaggc ggccatgcgg cgcgcgacct gccggccgac gcggccgtag ccgacgatgc
     3481 ccagggtgcg gcccgccagt tcgacgccgg cgaactcgga ccggcgccac tggccgtcct
     3541 cgaggctgcg ggcggcggcg gtgatgtggc gcgccgaggc gacgatgagc ccgacggtga
     3601 gttcggcgac gctcacggtg ttggagccgg gggcgttgac cacggcgatg ccgcgggctg
     3661 cggctgtgat ggtgtccacg ttgtcgaggc cgacgccggc ccgcccgacg acgcgcagcc
     3721 ggggtgcccg gtcgagcacc tcggcgtcga cccgggtgcc gctgcggacg atcagggcgt
     3781 cggcgtcggt cagagcggcg aacagggcct tgcggtcacg gccgtcgagg tgccggacgt
     3841 cgcacgtgga cttcaggacg gcgagggtct cgccgggcag tgcgtcactc acgatcacga
     3901 tcggtgaggg cacggatctc tccttgagga ggtgtggagc cgcgaggaag ggctctacta
     3961 atccaaacgt ttggtttgtt ttcaaaacaa gcctaacggt cggttggttt tcgggaaagc
     4021 cgcgttccat gtggcctgca ccactccccc cggcggtcca ccggggggcg ggcgtcaggt
     4081 caactgctcg cggtaccagg cggcatgggc cgccagaccc gcggagagac cggtctccgg
     4141 cgcgtggtcg cccacggtgc gcagccgggt cgcgtcgaac cagtggtcga cgccgaacag
     4201 gtccaggaga tggccgtcgc ccccggcggc gaccatctcc gcccgggccc gttcgagcgg
     4261 ctgaccaccc gtcaccagcg ggaagccgag gtgacggtgc gtagcgacga ccagttcgcg
     4321 gaggctgacc ggccggggag gacaggcatg gaagactccg gccgcccggc gcggcggcgt
     4381 ggtcacggcc atgcgcgcga gggacgtggc gagactgccg acctccgtga gcgacagcag
     4441 cgcggagccg tgcccgggca gtcccgagcg cagagcacgc agcatccgca gcaggctcgg
     4501 catcacccac cggtcacccg tcccgtagac gaggtacggg cgcaggacgg caccgcccgc
     4561 ctccagtacg aaccgctcgc cggacaggcg gctgcggctg gtgggcgaga cgggacgcgg
     4621 ctccacggcg ccttcggcgg cctcgcggaa cggaccgttc ccgtacacgg ccgccgtgcc
     4681 cagccgtacg aacttgccca cgcccgcccg gccggcctcg gccacgaggt ggcgtgttcc
     4741 gtcggtgttc accgccacgc cgctcgcctc gtcccggccg acgtgggagg cgaggtgcag
     4801 gacgcagtcc acgtcgcggc agataccgcg cagggtggcg gggtccgtga ggtccccgtc
     4861 cacgacctcg acgcccggcc cgcgtaccag ggggcgccgg tgggccagca ggcgcacccg
     4921 cgcccccgag gaccgcagga gcgggacgac cgcgccgccc acgaagccgg atccgccggt
     4981 gacgaggacg cgcggacggt tcaccggcgg gtgcgcgagg tcgagggcgc gcggtgggcg
     5041 acgaggtcgc actcgtaggc gacggcgccg ttctgctcgg cggtcacccg cacctcgtac
     5101 gtgccgacgc cggtcatggc cagccgtgcc cgcaccaggc agggggcgtc gaactccacg
     5161 taccggtgga agcggttggt catcgcgggc gccaggatcg cgccggggcc cgcgaggcgt
     5221 tgggccgcct gccgggcggc ctccatcagg agcatcccgg gcacgtggtc ggtggggtgg
     5281 tcgaagaggg tcgggtgcgc ccagttgacg cgcagcggcc aggccccctc gtacgggtcc
     5341 gtggtgtcgg cgccggccgg cggccggccg agcaccacat cggcctcgac ggcccggccg
     5401 acctcgtgcg gaggcaaagg cgcgggctgc tcgggcaggc ggacgggcac ctcggcgtac
     5461 gctccccgca gccgccggta gacggagcgc cgcaggcagt tgagcgtcat ccggccgtgg
     5521 cccaggcgca ccgcgcccca ggcgagcccg acgttcatcg tcatcccggc gaggaggtcg
     5581 ccgcggagcg tcacatccgt gcagtcgatg gtcagttcca cgttgaccgg ccggtcgtcc
     5641 agacgcaggg cgtgcgggga gagactgaag tccagctccg tcatgagggt cgggtggtcg
     5701 cagggcagct cgtagaaggc gtgggcgacc agcgaggacg tctgccggat actctcggcg
     5761 gccagcagcg gatcgtgcca gcggtggccg acggggccgt agaagccatg gccgcgcggc
     5821 cattgggcgc ccactttgaa gcggtgcggg ccatggcgta tccagtcggt cagcagcacc
     5881 tcgccgatgg cccggcggtg gacgagatcg cgcggcgccg tccggtcgaa ggacaggggg
     5941 cgtaagcgct cggtggcgag cggcgggggg caggcggtga ggacggaagg agaggagagg
     6001 tcgaccgaca tgtcacacgg ctcctgatct gcgctgtttc ccgaagacac ccggcggccg
     6061 ccgggtgggg gtgatcgtgg tacagcttgt cgaagactag aatacctaac gtacggtttg
     6121 ttttaaacac ctctcaccag gctcttcacg cgcctggcac gacccggaaa caaggaagtc
     6181 acgcgatggc ccaacaagaa cgagcgatcg aaacgcgccg gagactcctg atcggggcgg
     6241 ccgaagtctt cgacgaacgc ggctacgcgg cagcgagcat caacgagatc caggagcgca
     6301 gcgggatcac caaaggggcc atgtacttcc acttcgcgtc caaggaggag atcgcgcggg
     6361 gcgtgatggc cgcgcagacc gagcttcccg tcgacacccg cagccgctcc gcttcaccga
     6421 tgcagcgggc catcgacctg acccatgaac tggcccacca gctgcgcacg aacgtcctgt
     6481 tccgcgcgag catccggctc acggtggagc aggggacgtt ccaggcgaac gactccgacc
     6541 tctatgactg gtggctgcgg gccttcggcc aggaactgac gcgtgcccgg gacggcgggg
     6601 agctgcgcga ggagctggtc cccgaggacg tggcggccag cgtggtgggc tccttcaccg
     6661 gcatccagat gctctcccac gtcatgtgcg accgccggga cctccacgag cgcctggccc
     6721 gctggtggga gcagatcctc cccggcatcg cgacgcggga ggccctgcgc cgggcgctgc
     6781 cgaccgggtc cccggaactc ggcctgaccg gcgggacctg agcggcggga gccgagcgga
     6841 ggggcggggc ccgggcgccc tacgccgcgg accccagctc ctccccgcac cggaggttcc
     6901 accgccccgc gcggccgctg agcacggtca gcgtcagggg ccgcacgtcc agccgccaga
     6961 acgcccgcgg cggcagcgcg agggcgtgga cgaccgccgc ccggatcacg acgggttcgg
     7021 tgacggccag cacacggccg tcgtcaccct cctcgttcgc ctcgtcgccc tctcccccga
     7081 ggccgtcgag ccaggcgccg acccggccga cgagcgcgag caccgactcc ccgccgtgcg
     7141 gcgcggcccc ggggtccgcc agccaggtgg ccacggcccc gggctcggcc gccgacagct
     7201 cgccgagcgt cctgccccgc cagcggccgg cgtccaggtc cgccggcgcg gcgagggccg
     7261 gggcgaagcc caaggcggcg gccgtctcac ggcagcggcg ggaaggggag gcgagcaggc
     7321 gggaggcgcg gggtacggcc gcggcgacgg cccgggcccg gccgagcccg gcctcgtcga
     7381 gcggggagtc gtcgtcgaaa cgggcctcgc gcagggccgg tccgggggcc ggggcgagca
     7441 gcatggcgcg tacggtcata cgacggattg tgccccgacg ccggccatgg aacggcgggc
     7501 gacggaagcg cgggatcgag cggaatcgca gcgcgagaat ttcggtacgc ctatacgatg
     7561 aaaaaggccc tctttcagga aaccgcgtcc catcgtttgc cctctctcca ggagaccccc
     7621 cgtgaagagg agcgacaacc gtgggcctta cgtcccagac cttcctgctg accctggtgg
     7681 tcatagccgt cgcggtgacc gcgctggtcc tgtggctgtg gccgcgggcc gcgaagaagg
     7741 ggcccctggc gttcgcctcc cgtctcggga tgctcgtgct cgtccagctc tgtgtcctgt
     7801 ccgtgctgct cgtcgccgcg aacaacgaat tcggcttcta ctccacctgg aaggaactcc
     7861 tcggccagaa ctcccgcaag caggccgtgc gcgccggttc gggcaacggc aagcggccgc
     7921 cggccctgac ggtcaagggg caggagcccg tcggcctcgg ccccaaggag aaggccggac
     7981 aggtggaccg gatcgagatc aacggcccgg tgaccgggct gagcatggac ggcttcgcct
     8041 atctcccgcc ccagtacttc cagaaggggc atgagaagga caccttcccc gtgatcgtgt
     8101 cgatcaccgg gcagcccggc cagtccatca atctcatcac caaggccaag gtcccccagg
     8161 ccgcctccaa ggccgtgatg gcgggccaga tgaagccgac gatcgtgctg atgctgcgcc
     8221 cctcggtggt ggcggaccgc gacaccaact gcacggacgt gcccggcggt ccgcaggcgc
     8281 tgaccttctt caaccaggac gtcccggtcg ccgtcaacga cgtctaccgc gtggacaaca
     8341 cccgtggcgg ctggggtgcg gtgggcaact ccaccggcgg ctactgcgcg ttgaagatgg
     8401 ccatgaccaa tccgtaccgg tacggggccg ccgccggtct gtccaccgac ctcttcgccc
     8461 gcgaggaccg tgacaccggc gacctctacg ccggcaacaa gcagatcaag aacgagtccg
     8521 atctgacgtg gcgtctggag cacatgcccc cgccgccggt ctccctgctc ctggccgcca
     8581 gcgagaaggg cgaggagaag tactacgcgc agatgcagaa gttcgccggg aaggccaagc
     8641 cccccaccaa ggtggagaag ctggtgcgcg aggagggcgg ccacaacttc gtcacctggc
     8701 gcgaggagta ccccgtggtg ttccgctggc tcgacaagga actcgccacc cgccgctgac
     8761 gcccgcgccc atggccgccc gcgcacacgg ccttcaccag cggaggggaa cggtatcggt
     8821 cggcgccacg tcccagccag tgtggcccga aagcgcacgg ctccgtacgc cgtacgcggg
     8881 ggaaggggcg tacggcatat ggacacggaa cgccgcaaac cactgctggc cggacgctac
     8941 cggctcatgg agcagctcgg ccggggcggc atgggcgtcg tctggctggc catggacgag
     9001 gtgctggacc gtgaggtggc cgcgaaggag gtccaggctc cggagcggat gaacgccgac
     9061 gacgtacgcg tcctctacgc ccggctgaaa caggaggccc gcgcggccgc ccgggtcagc
     9121 caccccaacg tcatcaccgt gcacgacgtg gtggagcagg agggccgccc ctggatcgtg
     9181 atggagctgg tgcgcgggca gtccctggac gccgtactcc gccaggaggg cgtgctgacg
     9241 cccaaggagg ccgcccgcat cggcgccctg gtgctccagg cgctgcgcgc cgcccacgcc
     9301 gtgggagtgc tccaccgcga cgtcaaaccg gccaacgtcc tgatggaggc cgggggccgg
     9361 gtcgtcctca ccgacttcgg catcgcgctc gtcgagggcg cgggcaccct cacccgcacc
     9421 ggtgacctca tcggctccgc cgagtacctc gccccggagc gggcgacggg caagcgcccc
     9481 ggcatcccct ccgacctctg gtcgctgggc accctgctgt acgtggcggt cgagggcgtg
     9541 tcgccgttcc ggcgcggcga cgccctcagc acgctgcggg ccgtggtcga cgacccgctg
     9601 gcgccgcccc ggcgcgccgg cccgctgacg ccgttgctgg aggggctgct gcggaaggac
     9661 cccgcacggc ggatgggctc ggccgaggcc gagcagctcc tggacgccgt cgccgacggc
     9721 cggctgacgg aacagccggg gtacgtgccc acgaccctcg acgagccggt gcgcgcccgg
     9781 acggagaccc cgtcccccct ccccctgcgg gagacgccac cgccgttcac accgacgccc
     9841 acgccacctc cgacgccgac gccgtcccgc gctcccacac ctactccgac tcccacgccc
     9901 gcgccgtcgc cgatgccgac gccgaggccc tgggccgggc cctcgcccgt cggctccccg
     9961 accccgccgc ccgtgccggt cgggctccag caggggggcc tcgccacgcc cctggtcgtt
    10021 cccgcctacg gcgatccgcg ccatcccggg tacccggtgg gaccggatcc ggatccggat
    10081 ccggtgccgg atcccgggcg gcggaaggcc gcctggatcg tggcggccgt caccgcgctg
    10141 gtcctggtcg ccgggggcac cgccggggtg atcgccttcc gggacgcggg cggcggcacg
    10201 ggaactcctg gcggcgggcc caccgcctcg gtctcccagg gcgcgggcag cgatcaggac
    10261 gggcagagcc aggccccggc caccggggcg acgggggacg acgggtccgg gaacccgtcc
    10321 cccaccccgt cccccaccta cgaaccatct cccagcgccc ggacctcgtg cggcggtggc
    10381 tgggtgacgt cgtgttagtc acgctatcgg gggtgtgagt gtgcaacgga ccaggatcgt
    10441 ctcattgctg gcggtgcccg tgctgctggc cggcgccggg ctgacggcct gctcgtcgga
    10501 cggggggaag ccgtcgaaga tcgccctgga ggcggcgggc gtggtggcgg cgcagccgtt
    10561 cgtccaggcc cccggggccg atatccaggg ggtcaaggcc gtcccggtga cgggaggcac
    10621 cacccgggcc gacgccaaag gggccttcgg cggcacgccc ggccagaccc actgcgaccg
    10681 ggccaagctg atcgaccagc tgacccagga cccggtgaag gggaaggcct gggccgacgt
    10741 gcggggcatc gccttcgaca ggatcaagga tcacatcaac ggcctcaacg aagtcactct
    10801 cgcccaggac accctggtcg agaaccacaa ctaccagggc gacggcaaga ccgtcgcgta
    10861 tctctccgtc ctccaggcgg gcgtcaccat ccttcaggac gactacggga ccccttccgt
    10921 gaagtgcaac tgcggcaatc cgctgggcaa gccggaggac gtcgaccgga aagggtccac
    10981 gtacaccggg accaagtggg agcgcttcga g
//
