LOCUS       NZ_CP029617            10228 bp    DNA     linear   CON 19-MAY-2024
DEFINITION  Streptomyces sp. WAC 01529 chromosome, complete genome.
ACCESSION   NZ_CP029617
VERSION     NZ_CP029617.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. WAC 01529
  ORGANISM  Streptomyces sp. WAC 01529
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8270461)
  AUTHORS   Waglechner,N. and Wright,G.D.
  TITLE     Evolution of GPA BGCs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8270461)
  AUTHORS   Waglechner,N. and Wright,G.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2018) Michael G DeGroote Institute for Infectious
            Disease Research, McMaster University, 1280 Main St West, Hamilton,
            Ontario L8S 4L8, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. May-2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 202.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003945545.1-RS_2024_05_19
            Annotation Date                   :: 05/19/2024 04:27:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,297
            CDSs (total)                      :: 7,207
            Genes (coding)                    :: 7,110
            CDSs (with protein)               :: 7,110
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 33 of 97
            Pseudo Genes (incomplete)         :: 81 of 97
            Pseudo Genes (internal stop)      :: 9 of 97
            Pseudo Genes (multiple problems)  :: 22 of 97
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:50:51
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6879572
            Orig. end                         :: 6889800
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029617.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Gerard D Wright, McMaster
            University, 1280 Main St West, Hamilton ON, Canada.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10228
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="25"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10228
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10228
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5228](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5228
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(265..1425)
                     /locus_tag="DMA15_RS30695"
                     /old_locus_tag="DMA15_30685"
                     /pseudo=""
     CDS             complement(265..1425)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983476.1"
                     /locus_tag="DMA15_RS30695"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="DMA15_30685"
                     /product="magnesium and cobalt transport protein CorA"
                     /pseudo=""
                     /transl_table=11
                     /translation="MAERRPRPASPAKKHAWRRATRPPAAPPPEETRPAPPSGPTAANR
                     EAGSVVEAALYREGVRVATPSSLADTFRELREQRDGMAWIGLARPTEDELLSLAAEFDL
                     HELAVEDAMEAHQRPKLERYGETLFVVLRAARYLDAPEEVDFG"
     misc_feature    1496..1511
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 22.67"
                     /tool="antismash"
     misc_feature    complement(1497..1510)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 21.19"
                     /tool="antismash"
     gene            1672..2874
                     /locus_tag="DMA15_RS30700"
                     /old_locus_tag="DMA15_30690"
     CDS             1672..2874
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016638566.1"
                     /locus_tag="DMA15_RS30700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMA15_30690"
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="WP_125515955.1"
                     /transl_table=11
                     /translation="MTKTTRARTGTAPVLGARALNRATLDRQLLLRRSRDLSVKDAVAH
                     LVGLQAQNVKPPYYALAARLEDFDPAELSALLEARELVRMVTLRSTIHLHTADDARTLR
                     PFVQDALARELGVFRDGLTGVDLHELAGIAREAVEAEPRTMKQLREILLVRWPGADPQS
                     LAVAARCLLPLVQVTPRGLWRRSGQVCLTTLEHWLGGTDAEAPSPDTPTPDATVLRYLA
                     AFGPASVKDMQTWARLTRMREVFERLRPRLRTFRDENGVELFDLPDAPRPAADTPAPPR
                     FLPEFDNLLLSHADRTRVVPAAYKGRTWKGNFAYCTFLLDGFLAGVWRLEEDARSGSAV
                     LTIEPFGKPGRAERDALTREAERMLTRIAETPAAAAAAAASSSASSSASYDIRFGVVVG
                     S"
     misc_feature    complement(2945..2957)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 22.13"
                     /tool="antismash"
     gene            3010..4551
                     /locus_tag="DMA15_RS30705"
                     /old_locus_tag="DMA15_30695"
     CDS             3010..4551
                     /NRPS_PKS="Domain: AMP-binding (37-410). E-value: 1.3e-78.
                     Score: 256.3. Matches aSDomain:
                     nrpspksdomains_DMA15_RS30705_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018541096.1"
                     /locus_tag="DMA15_RS30705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMA15_30695"
                     /product="AMP-binding protein"
                     /protein_id="WP_125515956.1"
                     /sec_met_domain="AMP-binding (E-value: 3.6e-84, bitscore:
                     281.5, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTPMTVTDFLDRAELGFAAAPGVIDEPHQPASVVPDSTYGRLGE
                     RIRAWQAGFDALGVGEGERVAVVSHNSARLLELLFAVPMSGRICVPVNFRLKPDEVDYV
                     VRQSGASVLLVDPELDDALSGVKARHRFVLGERTETDLMRFGVEPRPWANPDEDATATI
                     NYTSGTTARPKGVQLTHRNIWVNGLTFGLHTRVWERDVYMHTLPMFHCNGWGMPFVMAG
                     LGVKQVVLRKVDGAEILRRVEEHGVTLMCGAPAVWNAVLDAATSWRGEVPGRDRVRIVC
                     AGAPPPSKMIQRVEAELGWEFTQIYGLTETSPLLTFNRAVPADAELPAEERARKLSRAG
                     LPALGVKLKVSDAGEVLARSNVVLESYWEKPEETAAALEDGWFHTGDGGTLDEADGHLT
                     ISDRKKDVIITGGENVSSIEVEDAIFSHPAVAEVAVIGVPHEKWGETIKALVVLAEGET
                     AGEADIIAHCKQRMAGYKAPTSVEFRDAIPRTATGKIQKFKLREPYWAGRGRGVN"
     aSDomain        3121..4239
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DMA15_RS30705_AMP-binding.1"
                     /evalue="1.30E-78"
                     /label="DMA15_RS30705_AMP-binding.1"
                     /locus_tag="DMA15_RS30705"
                     /protein_end="410"
                     /protein_start="37"
                     /score="256.3"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="STYGRLGERIRAWQAGFDALGVGEGERVAVVSHNSARLLELLFAV
                     PMSGRICVPVNFRLKPDEVDYVVRQSGASVLLVDPELDDALSGVKARHRFVLGERTETD
                     LMRFGVEPRPWANPDEDATATINYTSGTTARPKGVQLTHRNIWVNGLTFGLHTRVWERD
                     VYMHTLPMFHCNGWGMPFVMAGLGVKQVVLRKVDGAEILRRVEEHGVTLMCGAPAVWNA
                     VLDAATSWRGEVPGRDRVRIVCAGAPPPSKMIQRVEAELGWEFTQIYGLTETSPLLTFN
                     RAVPADAELPAEERARKLSRAGLPALGVKLKVSDAGEVLARSNVVLESYWEKPEETAAA
                     LEDGWFHTGDGGTLDEADGHLTISDRKKDVIIT"
     CDS_motif       3490..3549
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DMA15_RS30705_0001"
                     /evalue="8.10E-07"
                     /label="NRPS-A_a3"
                     /locus_tag="DMA15_RS30705"
                     /protein_end="180"
                     /protein_start="160"
                     /score="21.5"
                     /tool="antismash"
                     /translation="ATINYTSGTTARPKGVQLTH"
     misc_feature    4875..4890
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 22.36"
                     /tool="antismash"
     gene            5001..5228
                     /locus_tag="DMA15_RS30710"
                     /old_locus_tag="DMA15_30700"
     CDS             5001..5228
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014157700.1"
                     /locus_tag="DMA15_RS30710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMA15_30700"
                     /product="type A2 lantipeptide"
                     /protein_id="WP_125518110.1"
                     /sec_met_domain="strepbact (E-value: 1.4e-28, bitscore:
                     97.9, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNTPQVETLEISDADLDNVSGGLVAQVAGNVTSTADSIAPVSGV
                     VAGVAGTVENVTGVNVGAVTGLASGLTAGL"
     gene            5412..6218
                     /locus_tag="DMA15_RS30715"
                     /old_locus_tag="DMA15_30705"
     CDS             5412..6218
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="DMA15_RS30715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMA15_30705"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_125515957.1"
                     /transl_table=11
                     /translation="MQFRQQALSKLQSPEELDLPVRYARPQGLLVLAVTLVVMAAASVW
                     AVTGSVSSTLRAPGILTHGQGSYVLQSPVTGQVTRVLAEEGKRVAADAPVLEVRTDRGD
                     RVVRALAAGRVTTLVAKIGTVVTTGADVATVERVAGPGDPLLAMLYVPADSAATVPVGA
                     SVDLTVQSVPTQEYGTLRGRVKAIGRAAQTRQKITGFLGDEELAGQFTKDGPPVAVLVR
                     LQKSPRATSGYTWSTSGGPPFALTSMTPATGALHLAEQRPIDWLLP"
     gene            6299..8542
                     /locus_tag="DMA15_RS30720"
                     /old_locus_tag="DMA15_30710"
     CDS             6299..8542
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018558043.1"
                     /locus_tag="DMA15_RS30720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMA15_30710"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_125518111.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 5.9e-38, bitscore:
                     128.4, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPPPGGRRRHRPEPPKRGRRAAPRPAAKGRRPKTVRTPTVLQMEA
                     VECGAAALAMVLAHHGRHVPLEELRIACGVSRDGSRASNVLKAARSYGLTAKGMQMEPA
                     ALAEVKAPAILFWEFNHYVVYDGMGRRLGRRGVHINDPDKGRRFVPSEEFDTSFTGVVL
                     VLEPGAEFRPGGRRPGVMKALPARLRGTTGTMLAALLASLLLVAVGAALPALSRTYIDL
                     FLIGHQTSLLGALFASMGAMVALTVVLTWLQQANLLRGRIISSTLGSARFFRHLLRLPV
                     TFYAQRSPADLVQRLASNDAVAETLARDLTAAGVDGVVVLLYAALLWTYDPQLTLVGVG
                     IALLNIVAMRVVIRLRATGTQKLRADSAKLTNTSYTGLQLIETMKATGGENGYFRRWAG
                     QHATTLEEQQRLGVPSAWLGVVAPTLATLNSALILWIGGLRAVEGHLSIGLLVAFQALV
                     TRFTAPITRLNGVAGRIQDFAADVARLKDVENFPVDSLYSRPEPAASTRRLKGHVTLDD
                     ITFGYSPLDKPLLTGFSLSVGPGQQVALVGGSGSGKSTVSRLISGLYSPWEGTIRIDGQ
                     RLEDIPRGALAASVSFVDQDVFLFEGTIRDNVALWDPSIPDDAVIAALEDACLYEVVAR
                     RPGGIHGRVEQDGRNFSGGQRQRLEIARALVRRPSVLVLDEVTSALDAETEQVIIDNLR
                     RRGCACVVIAHRLSTVRDSDEIVVLDHGQVVERGRHEHLVAAGGPYADLVKEY"
     misc_feature    8832..8845
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.87"
                     /tool="antismash"
ORIGIN
        1 tccccgagta cgtcccagag aacctcgccc tgacatgaga gaacgggtgg cacagcacac
       61 gctgtgccac ccattctcgt tacgttgcgt agccatgcgc tgatgagggc agctgcgccg
      121 ttagacgtgg aacagggtgg gcgcgagaga agcgactgga gcagcggagc ggcgcattct
      181 ccccagattc tccccagagg gccagaacga ccgctccgta acgttcgcgg acgcgaatcg
      241 atgtcggcgt acccggctaa acctctacag ccagtcccgt ttcttgaaga tcacgtacag
      301 gctcgaacac accaatgcca tcagcaggat tgcgaaagga tagccgtaca cccagtgcag
      361 ctcgggcatg tgatcgaagt tcatgccgta gatcgttccc acgagtgtgg gtgcaaacag
      421 aatggccgcc cacgacgaga tcttcttgat ctcctcgttc tgttcgaagc ccgcctccgc
      481 cagcgcccgc atctccgcgt tctgctgctg cgtcaccagc gtcgcgttga ccgtgaggat
      541 gtcggtcagg gcctggcgga agccgtcgac gcgttcgctg gtgtgggtga cgtggtcggc
      601 cacgtcccgt aggtagcgct ggagttcctc gtccgtgccg tacttcgcga agcccgccat
      661 cagggcgtgc agcatgccga ccagggggcg ggtggcgcgc tggaactcga ccatttcgcg
      721 cgagagttcg tagatgcggc gggagacctc cgggtcgccg ctgaagacct cggtctcgat
      781 ctcgtcgatg tcgttctgga cgcccgcgac gaccggggcg tagccgtcga ccaccgcgtc
      841 gaggatcgcg tagagcaccg cctcggggcc gagtttgagg aggtcggggg tctcctccat
      901 gcggcggcgg accccggaca ggtcgggggc cgcggcatgc cggaccgtga tgacgaagtc
      961 ggggcccacg aagacgtgca actagccgaa gtcgacctcc tcgggggcgt cgaggtagcg
     1021 ggcggcgcgc aggacgacga agagcgtctc gccgtaccgc tccagcttcg ggcgctggtg
     1081 ggcctccatc gcgtcctcga cagccagctc gtgcaggtcg aactcggcgg ccagggaaag
     1141 gagttcgtcc tccgtcggcc gggcgaggcc gatccacgcc atgccgtccc gctgctcgcg
     1201 cagctcacgg aacgtgtccg cgagggagga gggcgtcgcc acgcggactc cctcgcggta
     1261 cagcgccgcc tccacgacac tgcccgcctc ccgattcgcc gcggtgggac cggagggcgg
     1321 tgcgggccgg gtctcctcgg gcggcggggc cgccgggggc cgcgtcgcgc gccgccacgc
     1381 gtgcttcttc gcgggcgatg ccggacgggg tcggcgctcc gccatgctgg ccgcctccag
     1441 gttgatgagt cgttgactcg agtcgagtgt gagtcgagtc gcgtgcgagt cggtgtcgaa
     1501 cgtatggtct agatggccat tgagcaggat atacgtccta aaaggcgcgg gcgggcagcg
     1561 cggcccctcg gagaagcggt cggcctgcgg cgtgcctcgt gaggcgcact acccccgcgc
     1621 cggccccctt gcggaccgta cgtgtccgca acccccgcta gcgtgacccg catgaccaag
     1681 acgaccaggg cccggaccgg caccgccccc gtgctggggg cccgcgcgct caaccgggcc
     1741 acgctcgacc ggcagcttct cctgcgccgc tcccgcgatc tgtccgtcaa ggacgccgtc
     1801 gcccatctcg tcggcctcca ggcgcagaac gtcaaaccgc cctactacgc cctcgccgca
     1861 cgcctggagg acttcgaccc cgccgaactc tccgcgctcc tggaagcgcg cgagctggtc
     1921 cgcatggtca ccctgcgctc caccatccat ctgcacaccg ccgacgacgc ccgcacactg
     1981 cggccgttcg tacaggacgc actcgcacgc gaactgggcg tgttccgcga cgggctcacc
     2041 ggcgtcgacc tgcacgaact cgccgggatc gcccgcgagg ccgtcgaggc cgagccccgc
     2101 acgatgaaac agctccgcga gatcctgctc gtgcggtggc ccggcgccga cccgcagtcc
     2161 ctcgccgtcg ccgcgcgctg cctgctgccg ctggtccagg tgaccccgcg cgggctgtgg
     2221 cggcgcagcg ggcaggtctg cctcactacc ctcgaacact ggctcggggg tacggacgcc
     2281 gaggcgccgt ccccggacac ccccaccccc gacgccaccg tcctgcgcta tctcgccgcc
     2341 ttcgggcccg cctccgtcaa ggacatgcag acctgggcgc ggctcacccg tatgcgggag
     2401 gtcttcgaac ggctgcggcc acgcctgcgg acgttccgcg acgagaacgg cgtcgaactg
     2461 ttcgacctgc ccgacgcgcc gcgccccgcc gcggacaccc cggctccgcc ccgcttcctc
     2521 cccgagttcg acaacctcct cctctcgcac gccgaccgca cccgcgtcgt gcccgccgcg
     2581 tacaaggggc gtacgtggaa ggggaacttc gcgtactgca ccttcctcct cgacgggttc
     2641 ctcgccggag tgtggcggct ggaggaggat gccaggagcg ggagcgccgt gctcaccatc
     2701 gagccgttcg ggaaaccggg gcgcgccgag cgggacgcgc tgacgcggga ggccgagcgg
     2761 atgctgacgc ggatcgccga gaccccggct gcggcagcgg cggcggcagc gtcttcgtcc
     2821 gcctcgtcct cggcctcgta cgacattcgc ttcggggtcg tcgtcggaag ctgagccgcc
     2881 gccgcgaccg cgggctgccg ccggccgcgg ccgccgtatg gcgacaccct gcttcggggg
     2941 tagtgggaca gcgtcacgga ctacacgatg ttacgtagcc gaagtggtcc cggatatcag
     3001 gagtgagcta tgcggacacc gatgaccgtc acggatttcc tcgaccgggc ggagctgggc
     3061 ttcgccgccg cccccggtgt gatcgacgag ccgcaccaac ccgcctcggt ggtaccggat
     3121 tccacgtacg ggcggctcgg ggagcggatc cgcgcctggc aggcggggtt cgacgcgctg
     3181 ggagtggggg agggggagcg cgtggcggtg gtgagccata actccgcccg gctcctcgag
     3241 ctgttgttcg ccgtgccgat gagcgggcgg atctgcgtgc ccgtcaactt ccggctcaag
     3301 ccggacgagg tcgattacgt ggtgcggcag agcggcgcct ccgtcctgct cgtggacccg
     3361 gagctggacg acgccctgtc gggcgtcaag gcacgccacc gcttcgtgct cggtgagcgg
     3421 accgagaccg atctgatgcg gttcggcgtc gagccgcgcc cctgggcgaa ccccgacgag
     3481 gacgccaccg cgacgatcaa ctacacgtcg ggaaccaccg cccgccccaa gggggttcag
     3541 ctcacccacc gcaacatctg ggtcaacggg ctcaccttcg gcctgcacac ccgcgtgtgg
     3601 gagcgggacg tctatatgca cacgctgccg atgttccact gcaacggctg gggcatgccg
     3661 ttcgtgatgg cggggctcgg tgtgaagcag gtggtgctgc gcaaggtgga cggcgccgag
     3721 atcctgcgcc gcgtggagga gcacggcgtc acgctcatgt gcggggcgcc cgcggtgtgg
     3781 aacgcggtgc tcgacgcggc cacgagctgg cgcggcgagg tgccgggccg tgaccgggtg
     3841 cggatcgtct gcgcgggcgc accgccgccg agcaagatga tccagcgcgt ggaggccgag
     3901 ctgggctggg agttcacgca gatctacggc ctgaccgaga cctccccgct gctcaccttc
     3961 aaccgggcgg tgcccgccga cgcggagctg cccgccgagg agcgggcgcg caaactctcc
     4021 cgcgccgggc tgcccgcgct cggcgtcaag ctgaaggtct ccgacgcggg cgaggtgctg
     4081 gcccgctcga acgtcgtact cgaaagctac tgggagaagc cggaggagac cgcggcggcg
     4141 ctggaggacg gctggttcca cacgggcgac ggcggcactc tcgacgaggc ggacggccat
     4201 ctgaccatct ccgaccggaa gaaggacgtc atcatcaccg gcggcgagaa cgtctcctcg
     4261 atcgaggtgg aggacgcgat cttcagtcac ccggcggtcg ccgaggtcgc ggtcatcggg
     4321 gtgccgcacg agaagtgggg cgagacgatc aaggcgctcg tcgtcctggc ggagggggaa
     4381 acggcggggg aggcggacat catcgcccac tgcaagcagc ggatggccgg ttacaaggcg
     4441 ccgacgagcg tcgagttccg cgacgccatc ccgcgcacgg ccacgggcaa gatccagaag
     4501 ttcaagctgc gggagccgta ctgggcgggc cgtggccggg gcgtgaactg acgacgcgtc
     4561 ggcgtcctca tcacgtcccc ttcggcgacg attcggcatg acgtcccctt cggcggcgat
     4621 tcggcacgcc ttcaatgaac ctgcacatcg aatgcgcagt ccggtcacgc aattcgacgc
     4681 atgcaccgca cgaattcccg tgcaggggcc atagaaagct ctctttggcg ggagagtgtc
     4741 cgatttgttg aagggagtac cgccgtgcgg tcgattccct tctgtgaatg ttgtgaagga
     4801 gctgccacct cgcggtgaaa ccgcctgcac gcagcggaga tgcgccgtca caacctcacc
     4861 tgacgggcgc ttcatcgatt ggatgtgcga gctgcgaacg tgctttgatc ctggcccacg
     4921 cggaattcgc cccgcaggta aggcggattc cgctggttgg aaacaccttt gcgacattca
     4981 tgagaagggc agctcacacc atgaacaaca ccccgcaggt cgagaccctc gaaatctccg
     5041 acgccgacct cgacaacgtc tccggtggcc tcgtcgccca ggtcgcgggc aacgtcacca
     5101 gcaccgccga ctcgatcgcc ccggtctccg gcgtggtcgc cggtgtcgcc ggcaccgtcg
     5161 agaacgtcac cggcgtgaac gtcggcgccg tcacgggcct ggcctccggc ctgaccgccg
     5221 gtctctgagc cttccgctca ccgagacacc gtgatccctg agccccggag ccactggttc
     5281 cggggctcac ggacgccacg ggcaagccgt cgcgggccca gtaggtcagg cgggcccagc
     5341 aggcccagcg ggccgagccg tcacaggcca accgggccaa gcggcccccg gcacagtaag
     5401 ggaagcgttc cgtgcagttc cgccaacagg ccctgtccaa gctgcagtcg cccgaggaac
     5461 tcgacctgcc ggtgcgctac gcccgccccc agggcctgct cgtcctcgcc gtcaccctcg
     5521 tcgtcatggc cgccgcgagc gtctgggccg tcaccggctc ggtctcctcg accctcaggg
     5581 cgcccggcat cctcacgcac ggccagggca gttacgtcct ccagagcccc gtcaccggcc
     5641 aggtgacccg cgtcctcgcc gaggagggca agcgcgtcgc cgcggacgct cccgtgctcg
     5701 aagtccgtac ggaccggggc gacagggtcg tacgcgccct cgccgcgggc cgcgtgacca
     5761 cactcgtcgc gaagatcggc accgtcgtca ccaccggcgc cgacgtcgcg accgtcgagc
     5821 gcgtggcggg ccccggggat ccgcttctcg ccatgctcta cgtgcccgcc gacagcgcgg
     5881 ccaccgtccc cgtcggcgcc tcggtggacc tcaccgtcca gtccgtgccc acccaggagt
     5941 acggcacgct gcgcggtcgc gtgaaggcga tcgggagggc ggcgcagacc cggcagaaga
     6001 tcaccggctt cctcggcgac gaggagctgg ccgggcagtt caccaaggac gggccgcccg
     6061 tggccgtcct cgtgcggctc cagaagtcac cccgcgccac gtccggctac acgtggtcca
     6121 cttccggcgg cccacccttc gcgctcacct ccatgacccc ggccaccggc gccctccacc
     6181 tcgccgagca gcgcccgatc gattggctgc ttccgtgacc gcaccccaca cttccccggc
     6241 cgcaccccac accccctccc cggccgcacc ccacccctcc ccggcacacc agcagcagct
     6301 gccacccccc ggcggccgcc gcaggcaccg ccccgagccg ccgaagcgcg gccgccgcgc
     6361 cgcaccgcgc cccgcggcga agggccggcg cccgaagacc gtccgcacgc ccaccgtcct
     6421 ccagatggag gccgtggagt gcggcgccgc cgccctcgcg atggtcctcg cccaccacgg
     6481 gcggcacgtg cccctggagg agctgcgcat cgcgtgcggc gtctcccgcg acggctcgcg
     6541 ggccagcaac gtcctgaagg cggcccgcag ttacggcctc accgccaagg gcatgcagat
     6601 ggagcccgcc gcgctcgccg aggtcaaggc gcccgcgatc ctcttctggg agttcaacca
     6661 ctacgtcgtc tacgacggca tggggcgccg cctcggccgc cggggcgtcc acatcaacga
     6721 ccccgacaag ggccgccgct tcgtgccctc cgaggagttc gacacgagct tcaccggcgt
     6781 cgtcctcgtc ctcgaaccgg gggcggagtt ccggccggga ggccgcagac ccggcgtgat
     6841 gaaggcgctc ccggcgcggc tgcgcggcac cacgggcacg atgctcgccg ccctcctcgc
     6901 cagcctgctg ctcgtcgcgg tgggcgccgc gctgcccgcg ctcagccgga cgtacatcga
     6961 cctgttcctg ataggccatc agacctccct cctcggcgcc ctgttcgcgt cgatgggcgc
     7021 gatggtggcg ctcaccgtgg tgctgacctg gctgcagcag gcgaacctgc tgcgcggccg
     7081 catcatctcc tccaccctcg gcagcgcccg cttcttccgg catctgctgc gcctgcccgt
     7141 cacgttctac gcccagcgca gccccgccga cctcgtgcag cgcctcgcct ccaacgacgc
     7201 ggtcgccgag accctggccc gcgatctcac cgcggccggg gtcgacggag tcgtcgtact
     7261 cctctacgcc gccctgctgt ggacgtacga cccgcagctc accctcgtcg gcgtcggcat
     7321 cgcgctgctg aacatcgtgg cgatgcgcgt cgtcatccgg ctgcgcgcca ccggcaccca
     7381 gaagctgcgc gccgacagcg ccaagctcac caacacctcc tacaccggcc ttcagttgat
     7441 cgagaccatg aaggcgaccg gcggcgagaa cggctacttc cggcgctggg cgggccagca
     7501 cgccaccacc ctggaggagc agcagcgcct cggggtgccg agcgcctggc tcggtgtcgt
     7561 cgcgccgacg ctcgcgaccc tcaacagcgc gctgatcctg tggatcggcg gcctgcgcgc
     7621 ggtcgaggga catctgtcga tcggcctcct cgtcgccttc caggccctcg tcacccgctt
     7681 caccgcgccg atcacccgcc tcaacggcgt cgcgggccgc atccaggact tcgccgccga
     7741 cgtcgcccgc ctcaaagacg tcgagaactt ccccgtcgac tccctctact cgcgccccga
     7801 gccggccgcg agcacccgcc gcctcaaggg ccacgtcacc ctcgacgaca tcaccttcgg
     7861 ctacagcccc ctcgacaaac cgctcctgac cggcttctcc ctgtcggtcg gcccggggca
     7921 gcaggtcgcg ctcgtcggcg gctcgggcag cggcaagtcg accgtgtccc ggctgatctc
     7981 ggggctctac agcccgtggg agggcacgat ccgcatcgac gggcagcgcc tggaggacat
     8041 cccgcgcggc gcgctcgccg cctccgtctc cttcgtcgac caggacgtgt tcctcttcga
     8101 gggcacgatc cgcgacaacg tcgccctgtg ggacccctcc atccccgacg acgccgtgat
     8161 cgccgccctt gaggacgcct gcctgtacga ggtcgtggcc cgcaggcccg gcggcatcca
     8221 cggccgcgtc gaacaggacg gccggaactt ctccggcggg cagcgccagc gcctggagat
     8281 cgcccgcgcc ctggtccgcc gtcccagcgt cctcgtcctc gacgaggtga ccagcgccct
     8341 ggacgccgag accgaacagg tcatcatcga caacctgcgc cgccgcggct gcgcctgcgt
     8401 cgtcatcgcc caccgcctga gcaccgtgcg cgacagcgac gagatcgtcg tactcgacca
     8461 cgggcaggtc gtggagcgcg gccgccacga gcacctcgtc gccgccggtg ggccttacgc
     8521 cgacctggtc aaggagtact gaggtgaccc aggagcagta cgtccccgcg cacgacacgg
     8581 tcgccgccgc cttcggcggg ctcggcacgc cggtcgactg cgcgggtctg cgcagcgtcc
     8641 acctggaggg cccgcaggtg ctgtggctcg tcgtggcggg cgcgctcgac ctgttcgccg
     8701 tcgacgccgt gcagcagggc cactggcact tcctcggccg cctcgaaccg ggcaccctcc
     8761 tcctcggccc cgtcgaaggc ccccagcaca ccctcgtcgg ccgccccctg gcgggctgcg
     8821 agctgcgccg cgtcccgctg cgcgagctgt accgccccga ctacggcgac gcctgggcgt
     8881 acgagcagca gtaccccacg gcgtacgaca cccaggacgc ggccctgagc ctccttgagc
     8941 acgccttcgc actcggcatc gggcgcggcc accgagtcct gttcgaagcc ccgctcgacg
     9001 gccgcaccct gggcgaggac cccgacggcg ccgtcaccga cgacgacatc ctctggatgc
     9061 cggtcgcgcc gggcagcgtg cggtacggct ccgcctacag cgccgaggcc gcgggcgatc
     9121 tgctcgtgga ccccgcgatg tggcagcgca tggtcaacca gcagtaccga ctgctcgcca
     9181 cgctcgaccg ctggatcgaa cgccttgagc gggcccacga ggaccgcacg gcggccggta
     9241 tcaaggcggg cgagaacgtc cgcgagcagg ccgaccggac cctcatcgcc tccatcgggc
     9301 gcgcgggcag gggcagttca cgccgagtcg gcgcgcgggg cggcgatgac gccacgtacg
     9361 cggcctgccg tcttgtcgcc gaggcggcgg gcatcacgct gaccggccgg gcggacggcg
     9421 gcgcggtgaa cgaccggctc gacccggtgg agcggatcgc ggtggcctcc cggatccgca
     9481 cccgcgcggt gcggctcgac gggcgctggt ggcgcgagta cgcgggcccg ctggtggggc
     9541 accgggccgc gagcggcgcg ccggtcgcgt tgctgtggcg gcgcggcggc tacgagtcgg
     9601 tgcatgccac ctccgagcgg cgcacccgcg tcgacaagac caacgcggac gagttcgagc
     9661 cgcaggccgt catgttctac cggccgctgc ccgagcggag actgacgccg tggcggctct
     9721 tccgcttcag cctgcggggc accgccggcg atgtgcgcaa cctcgcgctc gcctcgctcg
     9781 tgacggtcgc gatcggtgcg ctggtgccga tcgcgacggg gcaggtgctc ggcgtgtttg
     9841 tgccgaacgc ggagaagagc ctcatcgtcc aggtctccgt cgccgtcatg atcaccagca
     9901 tcgtctcggc ggcgttcatg ctgctccaga acctcaccat cctgcggctg gagggccgca
     9961 tggagagcgc gctccaacca gccgtctggg accggcttct gcggctgccc acccgcttct
    10021 tcacctcccg ctccaccggt gaactggcga gcgcggccat gggcgtcagc gccattcgcc
    10081 gggtcctttc gggcatcggc cccgtcgccc tccagtcgag cacggtcggc gcgatgaacc
    10141 tcgtcctgct gctgctctac agcgtccccc tcgccctggc cgcgctggcg atgctcgccg
    10201 tcatcgccac cgtcttcctc gccctcgg
//
