LOCUS       NZ_CP029617            10408 bp    DNA     linear   CON 19-MAY-2024
DEFINITION  Streptomyces sp. WAC 01529 chromosome, complete genome.
ACCESSION   NZ_CP029617
VERSION     NZ_CP029617.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. WAC 01529
  ORGANISM  Streptomyces sp. WAC 01529
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8270461)
  AUTHORS   Waglechner,N. and Wright,G.D.
  TITLE     Evolution of GPA BGCs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8270461)
  AUTHORS   Waglechner,N. and Wright,G.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2018) Michael G DeGroote Institute for Infectious
            Disease Research, McMaster University, 1280 Main St West, Hamilton,
            Ontario L8S 4L8, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. May-2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 202.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003945545.1-RS_2024_05_19
            Annotation Date                   :: 05/19/2024 04:27:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,297
            CDSs (total)                      :: 7,207
            Genes (coding)                    :: 7,110
            CDSs (with protein)               :: 7,110
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 33 of 97
            Pseudo Genes (incomplete)         :: 81 of 97
            Pseudo Genes (internal stop)      :: 9 of 97
            Pseudo Genes (multiple problems)  :: 22 of 97
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:50:51
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4382143
            Orig. end                         :: 4392551
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029617.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Gerard D Wright, McMaster
            University, 1280 Main St West, Hamilton ON, Canada.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10408
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="13"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10408
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10408
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5408](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5408
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(628..1770)
                     /locus_tag="DMA15_RS19800"
                     /old_locus_tag="DMA15_19785"
     CDS             complement(628..1770)
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016644663.1"
                     /locus_tag="DMA15_RS19800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMA15_19785"
                     /product="glycosyltransferase"
                     /protein_id="WP_125514131.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 4e-33, bitscore:
                     113.2, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPRILHVSQPVDGGVARVVTELAAAQAAAGLDVHVACPAEGTLPR
                     ALDAAGRGVRGHPWAASRSPGPGLRTEVAELNRVIDAVRPTLVHAHSAKAGLAARLALR
                     GRVPTVFQPHAWSFEAVGGGTALLARFWERRAARWTDRVVCVSEAEWITGRRAGIAGSY
                     VVVPNGVDVDHFSPGASRRARDRLLPTDFAPAPLVVCVGRLCRQKGQDVLLRAWASVTA
                     RVPGARLVLVGDGPDAGRLRAGADASVTFAGAARDTADWYRAADVVVLPSRWEGMALAP
                     LEAMACGRPVVVTDVNGARESLPSAGRAHGLVPPEDPAALARALVALLRDAELRDALGR
                     RGRRHVLDHHDARRAADAIDVVYRELLGAAPTKYRECTTT"
     gene            2089..2328
                     /locus_tag="DMA15_RS19805"
                     /old_locus_tag="DMA15_19790"
     CDS             2089..2328
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056482.1"
                     /locus_tag="DMA15_RS19805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMA15_19790"
                     /product="chaplin"
                     /protein_id="WP_125514132.1"
                     /transl_table=11
                     /translation="MSRTTKALALSAVAAAAVAGSAGVAAADAGAQGAATNSPGVLSGN
                     ALQVPVHIPVNVCGNSINVIGLLNPAFGNTCVND"
     misc_feature    complement(2453..2471)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.51"
                     /tool="antismash"
     gene            2615..2893
                     /locus_tag="DMA15_RS19810"
                     /old_locus_tag="DMA15_19795"
     CDS             2615..2893
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016644659.1"
                     /locus_tag="DMA15_RS19810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMA15_19795"
                     /product="hypothetical protein"
                     /protein_id="WP_125517890.1"
                     /transl_table=11
                     /translation="MKALKAAAVLAGSIAIAGSAAPAFAGGLTPTSLNGGLETLAGQPL
                     LDTKPVSTNMLDTENENSVVNTVNDTAEGLNAGGGPTKMLGGLPLAK"
     gene            3120..3617
                     /locus_tag="DMA15_RS19815"
                     /old_locus_tag="DMA15_19800"
     CDS             3120..3617
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008746418.1"
                     /locus_tag="DMA15_RS19815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMA15_19800"
                     /product="DUF5949 family protein"
                     /protein_id="WP_125514133.1"
                     /transl_table=11
                     /translation="MTSTSEQRPFQATDLGTLAVLAWSGEHPDEEQDMPFLLAYSLGDT
                     EGGPEATAEAMRVLLRDTGLPVGGPVVDGSRNPSLPVSLLVETDQAVVSMPHLHAQCVV
                     PPEWLAAVKQRGHAYFMVTTRAWPDARPGEPVDEKRLAEFAGDEETLLNSGHCLLPARS
                     LR"
     gene            3617..4282
                     /locus_tag="DMA15_RS19820"
                     /old_locus_tag="DMA15_19805"
     CDS             3617..4282
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010988243.1"
                     /locus_tag="DMA15_RS19820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMA15_19805"
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="WP_125514134.1"
                     /transl_table=11
                     /translation="MSTTGLPTRVPRQAVGGPGASDAAPEDGPHRPEGGRWFGLLLVVT
                     GAAGLLASWVITLDKFKLLQDPDFTPGCSLNPVVACGNIMESEQASAFGFPNPMLGLAA
                     YAVVIGAGAGVLAGARFPRWYWLTFQAGTLFGVAFCTWLQFQSLYRVNSLCLWCCLAWI
                     ATTLMFWYVTSHNVRRGFLPAPREVRAFLGEFTWTLPALHIGVIGMLVLTRWWDFWTS"
     gene            4399..4905
                     /locus_tag="DMA15_RS36730"
                     /old_locus_tag="DMA15_19810"
     CDS             4399..4905
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018568413.1"
                     /locus_tag="DMA15_RS36730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMA15_19810"
                     /product="hypothetical protein"
                     /protein_id="WP_164551792.1"
                     /transl_table=11
                     /translation="MKKSTRGALAAVIACAAAAGGSASATAAEQSEVTVPLHGVEYALG
                     MQAPEITLGLPVPLPSGSPEGPDYVEGQLLPDHVLPRVPVNNSLPSARVKAPLSDVVGD
                     HSVDAVEAELSASDVRATTPGAGLGLPLTAPRGELWGLPALQLPSAGLHAPRLSAQPTT
                     ALGLT"
     gene            5001..5408
                     /locus_tag="DMA15_RS19830"
                     /old_locus_tag="DMA15_19815"
     CDS             5001..5408
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF017995.4"
                     /locus_tag="DMA15_RS19830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMA15_19815"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_206302780.1"
                     /sec_met_domain="melC (E-value: 2.5e-42, bitscore: 143.1,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRRHLLGVAASALLAAGSVFRLAQAPREASPPVALDDPPSGEPL
                     PGAPLPGEPSFDEMYRGRRIQGESVASRDPHAPGGWSVTVDGRPLGLMRRADGGYLSTV
                     DHFQSYATPLAAARAAVDELGPSQALSGRGH"
     gene            5446..6318
                     /locus_tag="DMA15_RS19835"
                     /old_locus_tag="DMA15_19820"
     CDS             5446..6318
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020116480.1"
                     /locus_tag="DMA15_RS19835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMA15_19820"
                     /product="tyrosinase family protein"
                     /protein_id="WP_125514136.1"
                     /transl_table=11
                     /translation="MAHTRKNQSSLSRAERRRFVDAVLKLKRRGEYDEFVRMHIDYYVS
                     DGEDRLRVAHMTPSFFPWHRRFLIEFERALQRIDSGVTVPYWDWTKDRSPAASLWGEDL
                     MGGNGRRSDRRVTTGPFAYRNGHWVIKEGVTDGEFLTRDLGRPHDPLDLPTRRDVAWAM
                     ADPRYDVPPWDSRAPRGFRNKLEGWTSASGKERWRNHNRVHRWVGGHMLGGGSVNDPVF
                     WLNHAFVDLLWDRWRRRHPRSAPYLPARPPGPGSSQYRRVVARHEPMPPWDVRPDDLLE
                     HKRLYTYGS"
     gene            complement(6376..6762)
                     /locus_tag="DMA15_RS19840"
                     /old_locus_tag="DMA15_19825"
     CDS             complement(6376..6762)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020136070.1"
                     /locus_tag="DMA15_RS19840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMA15_19825"
                     /product="chaplin"
                     /protein_id="WP_125514137.1"
                     /transl_table=11
                     /translation="MSRIAKAAAVTLGTGAIVLGGSGLASADAGAQGSAVGSPGVLSGN
                     LLQVPIHVPINLCGNTVDIIGVLNPAFGNTCINNGGGHHKDNGHHNGHGKGHHGKGHDK
                     GHGKGHGKGKGHHGGGHHKGGYGG"
     misc_feature    6768..6785
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 19.29"
                     /tool="antismash"
     misc_feature    6814..6829
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.64"
                     /tool="antismash"
     misc_feature    complement(6815..6828)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.1"
                     /tool="antismash"
     misc_feature    6871..6882
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.17"
                     /tool="antismash"
     gene            6984..7265
                     /locus_tag="DMA15_RS19845"
                     /old_locus_tag="DMA15_19830"
     CDS             6984..7265
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="DMA15_RS19845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="DMA15_19830"
                     /product="hypothetical protein"
                     /protein_id="WP_125514138.1"
                     /transl_table=11
                     /translation="MTSARRLIAAVSLAAGAAALATPTAHAAGDALGPGNISVQGTIND
                     LQTAGMPEEHRALVPKIDQQLGGLSELNKLQALVEPVAPLMNLVPAIA"
     gene            complement(7281..7928)
                     /locus_tag="DMA15_RS19850"
                     /old_locus_tag="DMA15_19835"
     CDS             complement(7281..7928)
                     /EC_number="3.4.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020135775.1"
                     /locus_tag="DMA15_RS19850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMA15_19835"
                     /product="M23 family metallopeptidase"
                     /protein_id="WP_125514139.1"
                     /transl_table=11
                     /translation="MDHDIEKAPEAPPTRRHALRVAAGILAVGAVPLTAPRASARSADV
                     CTGEYDAEFEEALAEADAQVPAEETRGLPPELGPGRYTLPLRRRFRVSARYGIRGDWLA
                     GHHTGIDLAVPRGTPVYAVGAGVVVLARWSGAYGNAVTVRMRDGHYAVFAHLSRVQVQQ
                     GTRLRTGARIGSSGATGRATGPHLHLEIRARRGYGSDIDPVRYLARRGARLL"
     gene            complement(8009..8194)
                     /locus_tag="DMA15_RS19855"
     CDS             complement(8009..8194)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="DMA15_RS19855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_125514140.1"
                     /transl_table=11
                     /translation="MSVHLRLLVSLVAMVIPLLVFSPSAAAPTGPAGRTGTVVGQVECD
                     WKYGRSCVVGGGMSFM"
     misc_feature    complement(8270..8283)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.77"
                     /tool="antismash"
     gene            8377..9591
                     /locus_tag="DMA15_RS19860"
                     /old_locus_tag="DMA15_19840"
     CDS             8377..9591
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1087: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010982572.1"
                     /locus_tag="DMA15_RS19860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMA15_19840"
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_125514141.1"
                     /transl_table=11
                     /translation="MKIDCVGGGPASLYLAILAKLQEPAHEVTVHERHAAGSSHGWGVT
                     FWPDLLAELHLHDPVSARAVAERSVRWRGGFAHVGDRTTAHDGDVGHAIGRHRLRTILA
                     DRAQELGVRLEFGVEIGGRAQLPDADLVVAGDGAHSRLRTESAAHFGTRVTTGANRFIW
                     LGTTRVFTAFTFAFVETGHGWIWCYAYGYSPEHSTCVVECSEATWTGLGLDRLGHGDAL
                     RLLEGLFADMLDGHALLGRTDIDGHARWQAFPTVTNESWSHGNLVLIGDAAHTTHYSIG
                     AGTSLALRDAACLARALGPAGPEQLPAALAAYERERKQALLPVQSAARHSAQWYENLPR
                     YIGLEPERMFALLGQRHSPLLPYVPPQLYYHLDQTVGRLPSLRRLKRSLGPRLARVLQS
                     RSPRG"
     misc_feature    complement(9727..9740)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: medium, score: 20.31"
                     /tool="antismash"
     misc_feature    10102..10115
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 17.99"
                     /tool="antismash"
     misc_feature    10382..10396
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.74"
                     /tool="antismash"
ORIGIN
        1 gcatgccgca gcgggggcgc cgcagcaggg ccgccgccac cgcccgcgcc tcaccgcaag
       61 ggccgaacac cagcacgggc cggggcctgc gcaggacgtc cgcccggcgc tgccagtgca
      121 ccagggcgcg cccgccgacg gcgagcaggc actgggcggc gcccacggtg gccagggccg
      181 acaggggcag cggcggcacg ggggcgggca gcgccggggc gaggacggcg gtgagggcga
      241 ggccgagcgc gatccgcccg ctcagcgagg gcacctcgtc gagggtggcg ggcagcgccg
      301 tgtacgcgta caggcgcccc tgcgcgttca gggtgaggac ggcgaccaga agcaggggcg
      361 ccagcacggc gtaccaccgc ggctcgtcga gcaggagcag ggcgagcagt gcggcggcgg
      421 agtcgaccgc caccagggcg gcgaccgaac ggcggtgccg ggaggggcgc cgcgcctgcg
      481 ccccggcgcg tgaaccccgc gcgccccgcg gcccgatgac aaagctgccg ggcgaggcgt
      541 gggcactccg cgaggcgtgg gtgtcctgcg aggcgtgggt gtcctgccgt gtcggacctg
      601 acgaggaggc aacggttcgt tccgcactca cgtggtggtg cactccctgt acttggtggg
      661 cgccgcgccc agaagctcgc ggtacaccac gtcgatcgcg tccgcggcgc ggcgcgcgtc
      721 gtggtggtcc aggacatgcc tgcggccccg cctgccgagc gcgtcgcgca gctccgcgtc
      781 ccgcagcagg gcgacgaggg cccgggccag cgcggcggga tcctcgggcg gtacgaggcc
      841 gtgggcgcgg cccgccgacg gcaggctctc ccgggcgccg ttcacatcgg tgacgacgac
      901 cggccggccg cacgccatgg cctccagcgg ggccagcgcc atcccctccc accgggaggg
      961 cagcaccacc acgtcggcgg cgcggtacca gtcggcggtg tccctggccg ccccggcgaa
     1021 cgtcacggac gcgtccgccc ccgcgcgcag ccggccggcg tccggcccgt ccccgacgag
     1081 gacgagccgc gcccccggca cccgcgcggt gaccgaggcc caggcgcgca gcaggacgtc
     1141 ctgccccttc tgccggcaca gcctgcccac gcacaccacc aggggagcgg gggcgaagtc
     1201 ggtgggcagc agccggtcgc gggcccgccg ggaggcgccg ggcgagaagt ggtccacgtc
     1261 cactccgttg gggacgacca cgtaggagcc cgcgatgccc gcacggcggc cggtgatcca
     1321 ttcggcctcg ctgacgcaga cgacgcggtc cgtccagcgg gccgcgcggc gctcccagaa
     1381 ccgggcgagc agcgcggtgc ccccgccgac cgcctcgaag gaccaggcgt gcggctggaa
     1441 gacggtcggg acgcgcccgc gcagggcgag ccgcgcggcg agcccggcct tggcgctgtg
     1501 ggcgtggacg agcgtgggcc gtacggcgtc gatgacgcgg ttcagctccg ccacctcggt
     1561 gcgcagcccg gggccggggg agcgcgacgc cgcccacggg tggccgcgca ccccgcgccc
     1621 ggcggcgtcc agcgcccgcg gcagggtgcc ctcggcaggg caggccacgt ggacatccag
     1681 ccccgccgcc gcctgagcgg cggccagctc ggtgacgacc cgcgccacac ctccgtccac
     1741 gggctggctg acgtgcagga tccgtggcat gacggcgttg gccttccgac gtgtgaagag
     1801 ctctgaccac agcggagatg agaggttcgc atcaattacg cgaatctggc gcgtatgact
     1861 gaaatgccct aaatagaagg gcaactgtag ccgctgcggg gcgccgctcc gtcaacctga
     1921 gtacgtgtgt cggattggtg aagggcatgg tgaatcggcg atcgcgtacg tgggggcccg
     1981 ccgcacatca ctcgtctggg cgcacaactc gccgtccggg tcggcagttg atccctgtat
     2041 acgggcaccc ggcccgtacg tcgcaccctt accaaggagc agcactccat gtcgcgtacc
     2101 accaaggctc tcgccctgtc cgccgttgcc gccgccgccg tggccggaag cgcgggtgtc
     2161 gctgccgccg acgccggcgc gcagggcgcc gccaccaact ccccgggcgt cctctccggc
     2221 aacgctctcc aggtgccggt ccacatcccc gtgaacgtct gcggcaactc catcaacgtc
     2281 atcggtctgc tgaacccggc gttcggcaac acgtgcgtca acgactgacg cgtcgtctga
     2341 ccgaacggcc gtccccgcga aggcgggggc ggccgttccg catgtggggg ccccggccgc
     2401 ccgtgccgcc gcccgtaccg ccgccgtata gaaacaggcc ggttgatatg agaatgcgat
     2461 gcgttcttca tttgagcggg tcggcgggaa ctttccggac caggggtttc cgcaccggcc
     2521 ttgtctcgtt agtcccggtg aatgagacgc caggcaaacg atgcctgaac ggtgctccca
     2581 tatggcggcg cgagtcgccg aagaaaggaa catgatgaag gccctgaagg ctgccgctgt
     2641 ccttgccggt tcgatcgcca tcgccggttc cgccgcgccc gcgttcgccg gcggcctgac
     2701 gcccaccagc ctgaacggcg gacttgagac ccttgccggc cagcccctgc tcgacaccaa
     2761 gccggtgagc accaacatgc tcgacaccga gaacgagaac tcggtcgtca acaccgtcaa
     2821 cgacaccgcg gagggcctga acgccggtgg cggcccgacg aagatgctcg gtgggcttcc
     2881 cctcgccaag taacccccgg ttcattcccc tttccgctgc ggtgtactgc cccgaacggg
     2941 aaaagggaac acgcgacgac gcaagggccg gccccctctt ccacgggccg gccctttcgc
     3001 gtgcccgcac gtcatcggag accgccgaat gaccccatcg tgtgatggaa gccccgcgct
     3061 cggccaattg ggtgacagcg gaagggaata gggccgccgc cgtctatacg gttcccctca
     3121 tgacctcaac cagcgaacag cgccccttcc aagcaaccga cctcggaacc ctggctgttc
     3181 ttgcctggag cggcgaacac cccgacgagg agcaggacat gccctttctg ctcgcctact
     3241 ccctgggcga cacggagggc gggcccgagg cgacggccga ggccatgcgc gtcctgctcc
     3301 gcgacacggg gctgcccgtc ggcgggcccg tggtcgacgg ctcgcgcaac ccgtccctgc
     3361 ccgtcagcct cctcgtcgag accgaccagg cggtcgtctc catgccgcac ctgcacgcgc
     3421 agtgcgtcgt gccgccggag tggctcgccg cggtgaagca gcgcggccac gcctacttca
     3481 tggtcaccac gcgcgcgtgg ccggacgcca ggccgggaga gcccgtcgac gagaagcgcc
     3541 tcgccgagtt cgcgggcgac gaggagaccc tgctcaactc cggccactgc ctgctgcccg
     3601 cgcgaagcct gcgctgatga gcaccaccgg actgccgacc cgggtgccgc ggcaggccgt
     3661 cggcgggccc ggtgcgtccg acgcggcgcc tgaggacggc ccccaccggc ccgagggggg
     3721 ccggtggttc ggcctgctcc tggtcgtcac cggcgcggcg gggctgctgg cgtcctgggt
     3781 catcacgctc gacaagttca agctcctcca ggacccggac ttcaccccgg ggtgcagcct
     3841 caacccggtg gtggcctgcg ggaacatcat ggagagcgag caggcgtcgg cgttcggctt
     3901 ccccaacccg atgctcggcc tcgccgccta cgccgtcgtg atcggcgcgg gggcgggcgt
     3961 cctcgccggg gcccgcttcc cgcgctggta ctggctgacg ttccaggcgg gcacgctctt
     4021 cggcgtcgcg ttctgcacct ggctgcaatt ccagtccctc taccgcgtca attcgctctg
     4081 cctgtggtgc tgcctcgcct ggatcgccac cacgctcatg ttctggtacg tgacgtcaca
     4141 caacgtgcgc cgcggatttc tccccgcccc acgcgaagtg cgcgctttcc tgggcgagtt
     4201 cacctggact ctccccgccc tgcacatcgg cgtcatcgga atgctcgtac tgacgcgctg
     4261 gtgggatttc tggaccagct gagcccggga aatccacggg aattggttca gtcgggcgaa
     4321 gtttgcgctg atgtcggccc aggatcgttg attcggtgac ggccgctcgg tcgtcacggt
     4381 gaaaggacga gacccgcaat gaagaagtca acccgaggcg ccctcgccgc ggtcatcgca
     4441 tgcgcggcgg cggccggcgg cagcgcctcc gccacggccg ccgagcagtc cgaggtgacc
     4501 gtcccgctgc acggcgtcga gtacgccctg gggatgcagg cgcccgagat caccctcggc
     4561 ctcccggtcc cgctgcccag cggcagcccg gagggccccg actacgtcga gggccagctg
     4621 ctgcccgacc acgtgctccc gcgtgtcccg gtcaacaaca gcctcccgtc cgcgcgggtg
     4681 aaggccccgc tctccgacgt ggtcggcgac cacagcgtcg acgcggtcga ggccgagctg
     4741 agcgcctccg acgtcagggc caccacgccg ggcgccggcc tcggtctccc cctgaccgcg
     4801 ccgcgcggcg agctgtgggg gctgcccgcc ctccagctgc ccagcgcggg gctccacgcg
     4861 ccgaggctga gcgcccagcc gacgaccgcg ctcggcctca cctgaccgtg gcgccgctgc
     4921 tgaaccgtgt gggcccggtc ccgagccggg cccacaccgc cccctgccag gagcccccgg
     4981 ccccacggct catcgaccgc gtgacgcggc gccacctcct cggcgtcgcg gcctcggcgc
     5041 tgctcgccgc cgggagcgta ttccgcctcg cacaggcgcc gcgcgaggcg agcccgcccg
     5101 tcgcgctcga cgacccgccg tcaggtgagc cactgcccgg cgcaccactg cccggcgaac
     5161 cgtcgttcga cgagatgtac cggggccgcc gcatccaggg ggagtcggtc gcctcgcggg
     5221 acccgcacgc gccggggggc tggagcgtca ccgtcgacgg gcggccgctc ggcctcatgc
     5281 gccgggcgga cggcggctac ctcagcacgg tcgaccactt ccagtcgtac gcgacaccgc
     5341 tcgccgccgc ccgggcggcc gtggacgaac tcggcccctc ccaagccctg agcggccgcg
     5401 ggcactgagc cggccgcacc gccccggacg gcagaacgga cggacatggc ccacacgcgc
     5461 aagaaccaga gcagcctcag ccgggccgag cggcgcagat tcgtcgacgc ggtcctgaag
     5521 ctcaagcgca ggggggagta cgacgaattc gtccggatgc acatcgacta ctacgtgtcg
     5581 gacggcgagg accgactccg ggtcgcgcac atgaccccgt cgttcttccc ctggcaccgc
     5641 cgcttcctga tcgagttcga acgggccctc cagcggatcg actccggtgt caccgtcccg
     5701 tactgggact ggacgaagga ccgcagcccg gccgcgtccc tttggggcga ggacctgatg
     5761 ggcgggaacg ggcggcggtc tgaccggcgg gtcacgaccg gcccgttcgc ctacaggaac
     5821 ggccactggg tcatcaagga gggcgtcacc gacggggagt tcctcacccg cgacctcggg
     5881 cgcccgcacg accccctcga cctgcccacc cggcgggacg ttgcctgggc gatggccgat
     5941 ccgcgctacg acgtgccgcc gtgggactcc cgcgcgcccc ggggcttccg caacaagctg
     6001 gagggctgga cgtcggcgtc gggcaaggag cggtggcgca accacaaccg ggtccaccgc
     6061 tgggtcggcg ggcacatgct gggcggcggc tcggtgaacg accccgtgtt ctggctcaac
     6121 cacgccttcg tcgacctgct gtgggaccgc tggcgccgcc gccacccccg ctcggccccc
     6181 tacctccccg cccggccccc cggccccggc tcgtcccagt accggcgcgt cgtcgcccgc
     6241 cacgagccga tgccgccgtg ggacgtgcgg ccggacgacc tgctggagca caagcggctc
     6301 tacacctacg gcagttaacc ccgggcagcg cgaagccccc ggtggaacgc tcaccggggg
     6361 cttcctcggc cgggatcagc cgccgtagcc acccttgtgg tggccgccgc cgtggtgccc
     6421 cttgcccttg ccgtggccct tgccgtgacc cttgtcgtgg cccttgccgt ggtggccctt
     6481 gccgtggccg ttgtggtggc cgttgtcctt gtggtggccg ccgccgttgt tgatgcaggt
     6541 gttgccgaat gcggggttca gcacaccgat gatgtcgacg gtgttgccgc acaggttgat
     6601 cggcacgtgg atcgggacct ggagcaggtt gcccgagagc acaccggggg agccgacggc
     6661 cgagccctgc gcaccggcgt ccgccgaagc caggccggag cccccgagca cgatggcgcc
     6721 ggtgccgagg gtgacggcgg ctgccttcgc gatgcgagac atcacgttct cctttgagtc
     6781 ggatagagcg actgctacgc gcagccgcat tcttctaacg tctgttctca cggcgaagta
     6841 acggcatacg acactccggc caccggtccg gaaatcgctt ttccgcgcgt cgagcggacc
     6901 cgaatgcgct attcggcgtt tctcatgagt gagaacggag ggaaagccgt gtccttggcg
     6961 ggggtccggc cgtttctgag gacatgacat cagcacgccg acttatcgcc gctgtctcgc
     7021 tggccgccgg agccgccgcc cttgccaccc cgacggccca cgccgccggt gacgcgctgg
     7081 ggccgggcaa catctcggtg cagggcacga tcaacgatct ccagacggca gggatgcccg
     7141 aggagcaccg cgccctggtg cccaagatcg accagcagct cggcggcctg agcgaactga
     7201 acaagctgca ggcgctggtc gagccggtgg cccccctcat gaacctcgtg ccggccatcg
     7261 cgtagccggt accaccaggg ctagaggagc cgggctcccc gtctggcgag gtagcggacc
     7321 gggtcgatgt ccgagccgta gccgcggcgg gcccggatct ccaggtgcag atgggggccg
     7381 gtggcccggc ccgtcgcccc gctgctcccg atgcgggccc cggtccggag ccgcgtcccc
     7441 tgctggacct ggacgcggga gagatgggcg aagacggcgt agtggccgtc ccgcatccgc
     7501 accgtcaccg cgttgccgta cgcccccgac cagcgtgcga gtacgacgac ccccgcgccc
     7561 accgcgtaca ccggtgtccc gcgcggcacc gccaggtcga tgcccgtgtg gtgcccggcg
     7621 agccagtcgc cgcggatccc gtagcgcgcg ctgaccctga agcggcggcg cagcgggagg
     7681 gtgtaccggc cggggcccag ctcagggggg aggccccggg tctcctcggc ggggacctgg
     7741 gcgtccgcct cggccagggc ctcctcgaac tcggcgtcgt actcgcccgt gcagacatcc
     7801 gctgatcgtg cggaggcgcg cggcgcggtc agcgggaccg cgccgacggc caggattccg
     7861 gcggcgacgc gcagggcgtg gcgccgcgtg ggaggcgcct cgggcgcctt ctctatgtcg
     7921 tggtccatgc ccgtcagctc actagcggcg gactcgacac gcatcccgtg ggccgccgtc
     7981 gctactccag gcggctcaat cggcccgatc acatgaagga catgccgccg cccacgacgc
     8041 aggacctgcc gtacttccag tcgcactcga cctggccgac gacggttccg gtacggccgg
     8101 cggggcccgt gggggccgcg gcggaggggc tgaacaccag cagcgggatc accatcgcca
     8161 ccagcgacac aagcagtctg aggtgaaccg acacgtccgt tctcctcgtg atcgtcgtat
     8221 cccgagacgt tccgatcctg ccccgatgtc actcatgatc gctattccat caccaagggt
     8281 gaccttggtg gcgggtgtga cgggggtgcg agggcggcgg cgacacggcg tcggcgttga
     8341 gccgtgacgc ggcgggcggc gcttacgttg gtcggcgtga agatcgattg cgtcggtggg
     8401 ggccccgcgt ccctgtacct ggccatcctc gccaagcttc aggaaccggc ccacgaggtc
     8461 accgtccacg agaggcacgc ggccggctcc agccacggct ggggcgtcac cttctggccc
     8521 gatctcctgg cggagctgca cctccacgac cccgtctccg cgcgggccgt cgccgagcgg
     8581 tccgtgcgct ggcgcggcgg cttcgcgcac gtcggcgaca ggaccacggc ccacgacggc
     8641 gacgtgggcc atgccatcgg acgccaccgg ctgcggacga tcctcgccga ccgcgcccag
     8701 gagctgggcg tccggctgga gttcggcgtc gagatcggcg gccgcgcaca gctgcccgac
     8761 gcggacctcg tcgtcgcggg cgacggcgcg cacagcaggc tccgcacgga gagcgccgcc
     8821 cacttcggca cccgcgtgac gaccggcgcc aaccgcttca tctggctcgg cacgacccgg
     8881 gtgttcaccg cgttcacctt cgccttcgtg gagaccgggc acggctggat ctggtgctac
     8941 gcctacggat acagccccga gcacagcacc tgcgtggtgg agtgctccga ggccacctgg
     9001 accggcctcg ggctcgaccg gctcggccac ggggacgccc tgcgcctgct ggaggggctc
     9061 ttcgccgaca tgctcgacgg ccacgcgctc ctcggccgta cggacatcga cggccacgcc
     9121 cggtggcagg ccttcccgac cgtcaccaac gagtcctggt cgcacggcaa cctcgtcctg
     9181 atcggcgacg ccgcgcacac cacgcactac tcgatcgggg ccggcaccag cctcgccctg
     9241 cgcgacgccg cctgcctggc ccgtgcgctg gggccggcgg gccccgagca gctgcccgcc
     9301 gcgctcgccg cctacgagcg cgagcgcaag caggccctgc tcccggtgca gagcgcggcc
     9361 cggcacagcg cccagtggta cgagaacctg ccccggtaca tcggtctgga gccggagcgg
     9421 atgttcgcgc tgctcggtca gcgccactcg ccgctgctgc cgtatgtgcc gccgcagctc
     9481 tactaccacc tggaccagac cgtgggccgc ctcccgtccc tgcgcaggct caagcggagc
     9541 ctcggccccc ggctggcgcg cgtcctgcag agccggagcc ctcggggctg agccggaagg
     9601 gggcggggcc ccggtgggtg cagtgcccac gcgttcaccc gaatggccct atcggcttcc
     9661 ttggccggtc cgggcggtcg gaacggcgtc ggcgccggga tggcgaagcc gcgtacggct
     9721 gcccccgcac gctgcgtgac gctccgcctt gcgcggcagg cgtcggcggc gcgggatggg
     9781 tacgaggcgt gcgtcgtcct tggcggattg gtttctgccc gtcgcatgct ccaagggagt
     9841 gatcacccat gacgcagggc gaggccttca ccaaccccga tgcgctcaag ggcctgacag
     9901 cctacgaccg gaccggcgag aagatcggca gcatcgaacg ggtctacctc gacgaccgca
     9961 gcggacgccc cgagtgggtg accgtgaaga ccggcctctt cggcatgaag gagagcttcg
    10021 tgccgctggc gggtgcccgg ctgcggggcg acgacctgca cgtcacggcc accaaggagg
    10081 ccgtgaagga ggcgccgagg gtggatgccg accaacacct cgaccctggc caggagcacg
    10141 agctgtacgc ccactacggc ctgacccggc cgggtgccgc gaccggcacc ggcaaggcgg
    10201 cgaccggcac cggtatgggc acggcgggca ccgcgggcac ggccggtctg gccggcgccg
    10261 gtgcgggcca gggccggaac gccggtgacc gcgagtacgc catgcgggcc ggtctgggcg
    10321 acgacaccaa ggaccaggag atggtgcggt ccgaggagcg gctgcgggtc ggcaccgagg
    10381 aggaagaggt cggccaggcc cggctgcg
//
