LOCUS       NZ_CP029617            13436 bp    DNA     linear   CON 19-MAY-2024
DEFINITION  Streptomyces sp. WAC 01529 chromosome, complete genome.
ACCESSION   NZ_CP029617
VERSION     NZ_CP029617.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. WAC 01529
  ORGANISM  Streptomyces sp. WAC 01529
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8270461)
  AUTHORS   Waglechner,N. and Wright,G.D.
  TITLE     Evolution of GPA BGCs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8270461)
  AUTHORS   Waglechner,N. and Wright,G.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2018) Michael G DeGroote Institute for Infectious
            Disease Research, McMaster University, 1280 Main St West, Hamilton,
            Ontario L8S 4L8, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. May-2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 202.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003945545.1-RS_2024_05_19
            Annotation Date                   :: 05/19/2024 04:27:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,297
            CDSs (total)                      :: 7,207
            Genes (coding)                    :: 7,110
            CDSs (with protein)               :: 7,110
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 33 of 97
            Pseudo Genes (incomplete)         :: 81 of 97
            Pseudo Genes (internal stop)      :: 9 of 97
            Pseudo Genes (multiple problems)  :: 22 of 97
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:50:51
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2486354
            Orig. end                         :: 2499790
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029617.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Gerard D Wright, McMaster
            University, 1280 Main St West, Hamilton ON, Canada.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..13436
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="phosphonate"
                     /region_number="9"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     cand_cluster    1..13436
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13436
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:8436](-)"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..8436)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     misc_feature    476..490
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
     misc_feature    810..819
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            complement(886..2046)
                     /locus_tag="DMA15_RS11155"
                     /old_locus_tag="DMA15_11150"
     CDS             complement(886..2046)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="DMA15_RS11155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="DMA15_11150"
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="WP_125512618.1"
                     /sec_met_domain="ATP-grasp (E-value: 5.3e-05, bitscore:
                     21.6, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVVCLGYRAGFVEAARRAGLDLHLVVDKIKPGLKGLSYTKVADL
                     SDAEEVLRAVTEHPGDKMSAVVTGHEHPMFSATVIRSVFGLPGDTDPARCLRFRDKRVQ
                     KRAVSPKIPTARCAYLNPSAPDYAALAEQLGEVFVAKPADGFGSQATEPIRGQEQLDDY
                     LRRHPARSDVSTVVESYVEGREIHVDGVWWDGRPAWTAVSGYLAPLMGWTKGEAVADAP
                     LGPAESELSARATRFAAEVLRELDAPDTVFHLEAFVRPDGELVLGEVGARPAGALTPEV
                     LRMTHGVDLYAATLDVGLGRAPELPVPPQEPAELFGWIYLVRDAGRQLTEDDFRDRFDV
                     VEVDYPAPENDRVGTYGRCGHAIVKAATHEGLVDGLRAIAEFNRSV"
     gene            complement(2043..2492)
                     /locus_tag="DMA15_RS11160"
                     /old_locus_tag="DMA15_11155"
     CDS             complement(2043..2492)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /locus_tag="DMA15_RS11160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMA15_11155"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_125512619.1"
                     /transl_table=11
                     /translation="MPVVVRQARRRDATAVARLIHDVYRPFAATFRPTALRWSAETVRA
                     CSDTWLIARAGNEWVGVVHQTPDPEGHTLDALAVTPHCRRKGVGTQLVAAVEDRALSLG
                     TRRMVIALRDGLAANIAFFTALGYRPTRPFPPSHHLYVKEIGARE"
     gene            complement(2492..3265)
                     /locus_tag="DMA15_RS11165"
                     /old_locus_tag="DMA15_11160"
     CDS             complement(2492..3265)
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018892809.1"
                     /locus_tag="DMA15_RS11165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMA15_11160"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_240659747.1"
                     /transl_table=11
                     /translation="MSHVPPSDASFDGLADNYDRTRPRYPSALFQHLVGLLPEAGPPTV
                     VDAGAGTGIALEGVLPHLPADAAVHAVDVSADMIRLGRKKFPEVEWHEGTAEQYLSSCA
                     PGSVHVLVAAQSYQWMDRAAYLREAARCLAPGGVCMVIQNNRDHAAGGFAAEYEDLLEE
                     LSPGYSRFYRSFDIADELGAAFSHTERRAAEWHQSLTVEEFVTMSSSSTQAQRAVAAVG
                     PVFYARVRDLCARHAKGGSVEIPYLSEAFYAVRPS"
     gene            complement(3262..3735)
                     /locus_tag="DMA15_RS11170"
                     /old_locus_tag="DMA15_11165"
     CDS             complement(3262..3735)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018892808.1"
                     /locus_tag="DMA15_RS11170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMA15_11165"
                     /product="hypothetical protein"
                     /protein_id="WP_240659748.1"
                     /transl_table=11
                     /translation="MRRVAGTVSTDDIIPARYKHMHTDPGRLAPHLFEAYLPGFVESVR
                     PGDAIVSDSVFGIGSSREQAVSTLLANGISLVIAPRFGRILFRNCWNLALPAIEADTGD
                     LSEGMTISVDLERGEIRSEGDVIAGFPKPPLFLLEMIASGGLLNRVKPSPSGA"
     gene            complement(3756..4991)
                     /locus_tag="DMA15_RS11175"
                     /old_locus_tag="DMA15_11170"
     CDS             complement(3756..4991)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018892807.1"
                     /locus_tag="DMA15_RS11175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMA15_11170"
                     /product="aconitase family protein"
                     /protein_id="WP_125512620.1"
                     /transl_table=11
                     /translation="MKRRLKSGTPLAEQIIADHRDHEDGPVEPGRIVTATPDRIYVQDG
                     NAPTVARLYEQYGFSTVFDPERVSFVFDHSVLVANVDMADRMKDADAFAARLGVDVRAR
                     GQGISHVIAAEAEWFTPGALVLGSDSHTCVGGAFGALGLGMGASDIVAAMVTGTTWLRV
                     PETVELRITGTPTRAARSRDVLLTLLRERGQEPFLYRSVEFTGAWARDLSSDASAGLAS
                     LGVELGAKCVFVPDAEGTIPTGTAQGVSRIEFDISGLQPTVALPHLPGNTVPLDEAAGT
                     PISYVFLGSCTNSRLEDIAEAAAVVRGHRVRQGTQFVVTPGSNDIYRAAMRAGHIDTLL
                     EAGAVVTPAGCGACVGTQGPLPARGENVLSTMNRNFRGRMGNPEANIYLSSPLVAAVTA
                     LCGAVPGVEDLP"
     gene            complement(5001..6131)
                     /locus_tag="DMA15_RS11180"
                     /old_locus_tag="DMA15_11175"
     CDS             complement(5001..6131)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: HMGL-like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1271: 2-isopropylmalate synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018892806.1"
                     /locus_tag="DMA15_RS11180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMA15_11175"
                     /product="hypothetical protein"
                     /protein_id="WP_125512621.1"
                     /sec_met_domain="HMGL-like (E-value: 2.8e-72, bitscore:
                     242.1, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRDDLIIEDTTLRDGEQTPGVALSAKKKVEIFNALVDAGVRWIEP
                     GIPTMGGEEVRALKEMLERRDEVTLIGWNRGVRGDVQRSIDLGFRAIHIGLPTSDNHLK
                     NSVRRDRTWLLSTAQDMVKMAKDQGCFVSISAEDIGRTEPDFLKEYAVAVAEAGADRLR
                     LSDTIGILSPSQYAERVRAIDSVADIDLQAHCHNDFGLAVANTLAAIEAGVRYFHVTVN
                     GIGERAGMADLAQVVLSLRQWYDVDLGIDTTQLTALSRLVSEACGIPASPWQPIVGPNV
                     FAHESGIHTTGMLRDSSTFEPYSPELVGGERKLLVGKHSGRGVIAHALGEGGADLDTET
                     LEALLHDVRAEAIAVGGTVSPERLRELADQTRAARR"
     gene            complement(6151..7497)
                     /locus_tag="DMA15_RS11185"
                     /old_locus_tag="DMA15_11180"
     CDS             complement(6151..7497)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1083: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018892805.1"
                     /locus_tag="DMA15_RS11185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMA15_11180"
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="WP_346428909.1"
                     /transl_table=11
                     /translation="MPAQRREQMIMGAYVSYGTGHHAASWRHPTSDPTATRNLDHYVEV
                     TRLAEQHLYDTLFLSDAPAVFNDDQAGFGGRVTFFEPLTLMSALAMVSRHIGLVATSST
                     TYKEPYNLAREYASLDLISRGRAAWNLVTTSKLAAAGNLGLASHPAHADRYGRAEEFID
                     VVRGLWDTWEDDAFPADKAQGRFYDPAKRHKLRHRGSFFSLEAELNVSRPPQGYPVIVQ
                     AGSSDAGRELAARTAEMVFTAQPDLDSAVAFAEDLDKRAAGHARLNPGIIILPGLTVYA
                     GRNESEAQDRVEELKSLIRPEFGLSMLSDLVGGFDLSSCDIEGPLPHLPPSNANTSRRA
                     LIEKLAYGERLNIRQLYEKMTTARGHLTLIGSYDAVAKEMLQWFESGAADGFNIMPPLL
                     PESLNDFSENILPRLQRHGVFKRRYTPGTLRQKLGLRRPENQYVTSAQR"
     gene            complement(7516..8436)
                     /gene="aepX"
                     /locus_tag="DMA15_RS11190"
                     /old_locus_tag="DMA15_11185"
     CDS             complement(7516..8436)
                     /EC_number="5.4.2.9"
                     /GO_function="GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA]"
                     /GO_process="GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="aepX"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231: carboxyvinyl-carboxyphosphonate"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR02320.1"
                     /locus_tag="DMA15_RS11190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMA15_11185"
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="WP_164551731.1"
                     /sec_met_domain="phosphonates (E-value: 9.5e-88, bitscore:
                     292.7, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 2.1e-56, bitscore:
                     189.8, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSISSVGNEIRLAALREALSREKFVRILEVHSPLSAVIAEHAQGV
                     GPTKRSFHGFWSSSLTDSALRGLPDIELLDSNLRLSWIDQIFSVSTLPLVMDGDTGGRV
                     SHFEYLVRAMERRGVSAVVIEDKSGEKRNSLLPGKGLHTMAPVEEFCEKISRGKAAQAG
                     TDFMIIARLEGLITGLPRQEVLDRAGAYVEAGADGLVIHSRSADESLVLDLARELRKRY
                     PHIPLVSIPTAYPSVTEQELHEAGFRLIIYANHMLRAATRAMEEVSHRILDHGRALEAG
                     EVCIETSKILSMPEDKRPIVRAPRH"
     misc_feature    complement(8702..8716)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.84"
                     /tool="antismash"
     misc_feature    complement(9642..9655)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: medium, score:
                     16.73"
                     /tool="antismash"
     misc_feature    9853..9862
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    11068..11081
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: medium, score:
                     16.82"
                     /tool="antismash"
     misc_feature    11517..11531
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.94"
                     /tool="antismash"
ORIGIN
        1 aacctgtccg cgtcgtggcc gagctggcag gccacgaacg cgccccagag gtccgccccg
       61 gtcgtcacgc gcagcagctc cccccacccc gcgccggccg gacgcatgcc gacctcaccg
      121 aagtacagct ccgcgccgtc gtacaggaac tccgcgtgga agacgccgtc caccgcgtcg
      181 agtgctccga tcacgcttcg ggtgagggcg gtcagccggg catgcatggc gtcgtcgcgg
      241 cagaggatgg acgtgcccca cgtgccggcc ttcttgagcg ccggccggtc gtactcggag
      301 acctccaccc aggagacacg gccgtcgtgg cagacgccgt cgcagtggaa ctcccgctgt
      361 accgcgacgg cctgctcgac cagccacagt tcgccgtggt ccgcgagcca ggcacgggcc
      421 tgggcgacgg gcatccggcg gacgtcccgt gaggcggagg aggcgatcgg cttcacgacg
      481 acctcggcca tggcccgcgt acgttccggc tcctcggcca ggaaatgccc ggagagccag
      541 gcttgtgggg agggcacggc ggcggcgagc ctcctgcgca tccgccactt gttcgtcacg
      601 ggcagtgtct gttcgtagcc gagccccggc aggtcgtacc ggctgcgcag gaacccggcg
      661 gcgagcatgg tgctctcgct cgtggccacc accgcgccga acggctgagc cgccatcagc
      721 gcgtcggcgt ggagcagaag ctcggggccg ggcgtcttct cccactgccg cgcctccgcg
      781 tcgaggcgga cgaactcgtg cgtgtcggag aaggcggcca tggcgcggcg ggcccaatcc
      841 ttgtcgcgtc ccagtacgag agcccgggtc ctgttcgtcg acacctcaga cgctccggtt
      901 gaactcggct atggcacgca gaccgtccac caggccctcg tgtgtcgccg ccttgacgat
      961 ggcgtgtccg caccgtccgt acgtgcccac acggtcgttc tccggtgccg ggtagtccac
     1021 ttcgaccacg tcgaatcggt cgcggaagtc gtcctcggtc agttgtcgtc cggcgtcgcg
     1081 gaccaggtag atccatccga acagctcggc gggctcctgg ggtggcaccg gcagttcggg
     1141 ggcccggccg agccccacat cgagggtcgc ggcgtagagg tcgacgccat gggtcatgcg
     1201 cagcacctcg ggtgtgagcg caccggccgg acgtgccccc acctccccga ggacgagctc
     1261 cccgtccggc cggacgaagg cttcgaggtg gaagacggtg tccggggcgt ccagctcgcg
     1321 caggacctcc gcggcgaacc gggtcgcccg ggcgctgagt tcgctctcgg ccggccctag
     1381 cggcgcgtcg gcgaccgcct cgcccttggt ccatcccatc agcggggcga ggtagccgga
     1441 gaccgccgtc cacgcgggac ggccgtccca ccacacgccg tcgacgtgga tctcgcgtcc
     1501 ctcgacgtac gactcgacca ccgtcgacac gtccgaccgg gccggatggc ggcgcaggta
     1561 gtcgtcgagc tgctcctggc cgcggatggg ctcggtggcc tgggagccga atccgtcggc
     1621 cggcttggcg acgaagacct cgccgagctg ctccgccagt gccgcgtagt cgggcgcgga
     1681 ggggttgagg taggcgcacc gggccgtggg gatcttgggg gagacggctc gcttctggac
     1741 gcgtttgtcg cggaagcgca ggcaccgcgc cggatcggtg tcgcccggca ggccgaacac
     1801 cgagcggatg acggtcgccg agaacatcgg gtgctcgtgc cctgtgacca ccgcgctcat
     1861 cttgtcgccg ggatgctccg tgacggcgcg caggacctcc tcggcgtcgg agaggtcggc
     1921 gaccttcgtg taggacagtc ccttcagacc aggcttgatc ttgtcgacga cgagatgcag
     1981 gtccaggccg gcgcgcctgg ccgcttcgac gaatcctgct cgatacccca gacagacaac
     2041 agtcactctc gtgcaccgat ctctttgacg tacaggtggt gggagggcgg gaacggtcgc
     2101 gtgggccggt atcccagggc ggtgaagaac gcgatgttgg cggcgagacc gtcgcgcagg
     2161 gcgatgacca tgcggcgcgt accgagggag agagcccgat cctccaccgc ggcgaccagt
     2221 tgggtgccca cgcccttgcg gcggcagtgc ggcgtcacgg ccagagcgtc cagcgtgtgc
     2281 ccctccggat ccggcgtctg gtgcaccacg cccacccatt cgttcccggc ccgtgcgatc
     2341 agccaggtgt cggagcaggc ccggacggtc tctgcggacc accgcagagc ggtcggacgg
     2401 aatgtcgcgg cgaacggccg gtagacgtcg tggatcagcc gggccaccgc ggtcgcgtcc
     2461 cggcgccgcg cctgccggac cacgacgggc atcacgacgg ccggaccgcg tagaacgctt
     2521 cgctgaggta cgggatctcg acgcttcccc ccttcgcgtg gcgggcgcac agatcgcgta
     2581 cccgggcgta gaagaccggt cccacggccg ccaccgcgcg ctgcgcctgg gtggaggagg
     2641 agctcatggt gacgaactcc tcgacggtca gcgactggtg ccactcggcc gcccggcgct
     2701 cggtgtgcga gaaggccgcg cccagttcgt cggcgatgtc gaagctgcgg tagaagcggc
     2761 tgtaaccggg tgagagttcc tccagcaggt cctcgtactc ggcggcgaac ccgccggccg
     2821 cgtggtcgcg gttgttctgg atgaccatgc agaccccgcc gggggcgagg cacctggccg
     2881 cctcgcgcag gtaggccgcc cggtccatcc actggtagga ctgggcggcg accaggacgt
     2941 ggacggagcc cggggcacac gaggagaggt actgctcggc ggtcccctcg tgccattcca
     3001 cctcggggaa cttcttcctg ccgagccgga tcatgtccgc cgacacatcc acggcgtgta
     3061 cggccgcgtc ggcgggaagg tgcggcagca ctccctccag ggcgatgccc gtgccggccc
     3121 cggcgtccac cacggtcggc gggcccgcct cggggaggag gccgacgagg tgctggaaca
     3181 gggcggaggg gtaacgcggc cgtgtccggt cgtagttgtc ggccaggccg tcaaagctgg
     3241 catcggaagg gggcacgtgg ctcacgctcc agacggtgag ggcttcacac ggttcagaag
     3301 accgcccgag gcgatcattt ccagcagaaa aagcggaggc ttcggaaacc cggcgatcac
     3361 gtctccctcg gagcggatct ctccgcgctc caggtcgacg gagatcgtca tcccctcgct
     3421 gaggtcgccg gtatcggcct cgatggcggg gagggcgagg ttccagcagt tgcggaagag
     3481 tatccggccg aagcgggggg cgatcaccag actgatcccg ttggccaaga gggtggagac
     3541 cgcctgttcc cgcgaactgc cgatgccgaa gaccgagtcg ctgacgatcg cgtcccccgg
     3601 ccggacactc tccacgaaac cggggaggta ggcctcgaac aggtgaggcg cgaggcgccc
     3661 gggatcggtg tgcatgtgct tgtagcgtgc cgggatgatg tcatcggtcg agaccgtgcc
     3721 ggcgacgcgg cgcacccggt gtgtcgatat gtgggtcacg gcaggtcctc cacgccggga
     3781 accgcgccgc acagcgcggt caccgcggcc acgagcggcg acgagaggta gatgttggcc
     3841 tcggggttgc ccatgcgccc gcggaagttg cggttcatgg tggagagcac gttctcgccg
     3901 cgggcgggca gcgggccctg ggtccccacg cacgccccgc agcccgcggg cgtgaccacg
     3961 gctccggcct ccagcagtgt gtcgatgtgg ccggcgcgca tggctgcccg gtagatgtcg
     4021 ttcgagcccg gcgtcaccac gaactgggtg ccctggcgca cgcggtggcc gcgcaccacc
     4081 gctgccgcct ccgcgatgtc ctccagtcgg ctgttggtgc aactgcccag gaagacatag
     4141 gagatggggg tgcccgccgc ctcgtcgagc ggcacggtgt tgcccggcag atgcggcagg
     4201 gcgacagtgg gctgaagacc gctgatgtcg aattcgatcc gggagacccc ctgagccgtt
     4261 ccggtcggga tcgtgccctc cgcgtcgggg acgaagacgc atttcgcgcc gagttcgacg
     4321 cccagactcg ccaggccggc ggacgcgtcg ctggacaggt cacgcgccca cgccccggtg
     4381 aactccaccg accggtagag gaacggttcc tggcctcgct cgcgcagcag ggtgaggagt
     4441 acgtcgcgtg accgtgccgc ccgcgtcggc gtccccgtga tccgcagctc gacggtttcg
     4501 ggaacgcgca gccaggtggt cccggtgacc atcgccgcga cgatgtccga ggctcccatg
     4561 ccgaggccga gcgccccgaa ggcgccgccg acgcaggtgt gggagtcgga cccgaggacg
     4621 agggcccccg gggtgaacca ttcggcttcg gcggcgatga cgtggctgat gccctggcct
     4681 cgggcgcgta cgtccacccc gaggcgcgcg gcgaacgcgt cggcgtcctt catcctgtcg
     4741 gccatgtcga cgttggccac gagaacggag tggtcgaaga cgaagctcac ccgctccggg
     4801 tcgaagaccg tcgagaaccc gtactgctcg tacagcctgg cgacggtcgg cgcgttgccg
     4861 tcctgtacgt agatccggtc gggcgtggcg gtgacgatcc ggcccggttc caccggcccg
     4921 tcctcgtggt cgcgatggtc ggcgatgatc tgttcggcga gcggggtccc gctcttgagc
     4981 cgccgtttca cagcccgtcc tcagcgccgc gcggcgcgcg tctggtcggc gagttcgcgc
     5041 aggcgctcgg gcgagacggt gccgccgacg gcgatggcct cggcccgcac gtcgtgcagc
     5101 agtgcctcca gcgtctccgt gtccagatcg gcaccaccct cgccgagggc gtgggcgatc
     5161 acaccgcggc cggagtgctt gccgaccagc agcttgcgct cgccgcccac cagttccggg
     5221 gagtacggct cgaaggtgga ggagtcgcgg agcatgccgg tggtgtggat gccggattcg
     5281 tgggcgaaga cgttgggtcc gacgatcggc tgccacggcg aggcgggaat gccgcaggcc
     5341 tcggagacca ggcgggacag ggccgtgagt tgggtggtgt cgatgccgag gtcgacgtcg
     5401 taccactggc gcaggctcag cacgacctgt gcgagatcgg ccatgccggc ccgctccccg
     5461 atgccgttga ccgtgacgtg gaagtagcgc acgcctgctt ctatcgccgc cagggtgttg
     5521 gccaccgcga ggccgaagtc gttgtggcag tgggcctgga ggtcgatgtc ggcgacgctg
     5581 tcgatggcgc gcacccgctc cgcgtactgg ctgggcgaca ggatgccgat ggtgtcggac
     5641 aggcggagcc ggtccgctcc cgcctcggcg acggcgaccg cgtactcctt gaggaagtcg
     5701 ggctccgtgc gtccgatgtc ctccgcgctg atgctgacga agcagccctg gtccttcgcc
     5761 atcttcacca tgtcctgcgc cgtggacagc agccatgtgc ggtcccggcg gaccgagttc
     5821 ttcaggtggt tgtcggaggt gggaaggccg atgtggatgg cgcggaagcc gaggtcgatg
     5881 ctgcgctgca cgtcgccgcg cacaccccgg ttccacccga tgagcgtcac ctcgtcgcgg
     5941 cgctccagca tctccttcag ggcccggacc tcctcgcctc ccatggtggg gatcccgggc
     6001 tcgatccacc gcaccccggc gtccaccagg gcgttgaaga tctccacctt cttcttcgcc
     6061 gacagagcca cgccgggggt ctgctcgccg tcgcgcaggg tggtgtcctc gatgatcagg
     6121 tcgtcgcgca tgctgatgct ctctttccgt ctatcgctga gcggaggtca cgtactggtt
     6181 ctcgggacga cgcagtccca atttctgccg aagggtgccg ggtgtgtagc gccgtttgaa
     6241 tactccgtgg cgctggagcc ggggcagaat gttctcggag aagtcgttga ggctctccgg
     6301 cagcagcgga ggcatgatgt tgaagccgtc ggcggcgccg ctctcgaacc actggagcat
     6361 ttctttggcg accgcgtcat agctgccgat gagggtcaga tggccgcgtg cggtggtcat
     6421 cttctcgtac agctggcgta tgttgagccg ctcgccgtaa gcgagcttct ctatcagtgc
     6481 gcgccggctg gtgttggcgt tgctcggcgg caggtgcggc aacgggccct cgatgtcgca
     6541 ggatgacaga tcgaatccgc cgaccaggtc cgaaagcatg ctgaggccga attcaggacg
     6601 gatgagcgac ttcagctctt cgactctgtc ctgcgcctcg ctttcgttcc ttccggcgta
     6661 gacggtgagg cccggaagga tgatgattcc ggggttgagc cgtgcgtggc cggccgcccg
     6721 cttgtcgagg tcttccgcga aggcgacggc ggagtccagg tccggttggg ccgtgaacac
     6781 catctcggcg gttctggcgg cgagttcccg gccggcatcg gaagacccgg cctgcactat
     6841 caccggatat ccctgtggcg ggcgggagac gttgagctcc gcttcgagtg agaagaagct
     6901 tccccgatgg cgcagcttgt gccgcttggc gggatcgtag aagcggcctt gcgccttgtc
     6961 cgccggaaag gcgtcgtcct cccaggtgtc ccagaggccg cgcaccacgt cgatgaactc
     7021 ctcggcccgg ccataccggt ccgcgtgcgc gggatgcgat gccagcccga ggttgccggc
     7081 ggccgcgagt ttggacgtgg tcaccaggtt ccaggccgcc cgccctcgcg agatgaggtc
     7141 gaggctggcg tactcgcggg ccaggttgta cggctccttg tatgtcgtcg aggaggtcgc
     7201 caccagaccg atgtgccggc tgaccatggc cagcgcactc atcagtgtca gcggttcgaa
     7261 gaaggtcacc cggcccccga agccggcctg gtcgtcgttg aagacggccg gtgcgtcgga
     7321 caggaacagg gtgtcgtaca ggtgctgctc ggcgagccgg gtcacttcga cgtagtggtc
     7381 gaggttgcgg gtggcggtcg gatcgctcgt ggggtgccgc caggaggcgg catggtgccc
     7441 ggtgccgtac gagacgtagg cacccatgat catctgttcc ctgcgctgtg cgggcatcgc
     7501 gacggctccg gtgggtcagt ggcggggggc gcgcacgatg ggccgcttgt cctcgggcat
     7561 cgagaggatc ttcgaggtct cgatgcacac ctcgccggct tcgagtgccc ggccgtggtc
     7621 gaggatgcgg tgactgacct cttccatggc ccgagtcgcc gcgcgcagca tgtggttggc
     7681 gtagatgatc aggcggaaac ccgcctcgtg aagctcttgc tcggtaaccg agggataggc
     7741 cgtcggaatc gacaccaagg ggatgtgcgg ataccgcttt cgcagctcgc gtgccagatc
     7801 gagcacgagg gactcgtccg cgctgcggct gtgaatgacc aggccgtccg ccccggcctc
     7861 gacataggct ccggcgcggt cgagcacctc ttgtctcggc agtcccgtga tgagcccttc
     7921 gagccgagcg atgatcatga agtcggtgcc ggcctgtgcg gccttccccc gggatatctt
     7981 ttcgcagaac tcctcgaccg gggccatggt gtgcagaccc ttgccgggca gcagagagtt
     8041 ccgcttttcc ccgctcttgt cctcgatcac cacggccgat acgccacgcc gctccatggc
     8101 cctgaccaga tattcgaagt ggctcacccg tccgcccgta tccccgtcca tgacgagagg
     8161 cagggtgctc accgagaaga tctgatcgat ccaggacaac cgcagattgc tgtcgagcag
     8221 ttcgatgtca ggaaggccgc gcaaggcgga gtcggtcagc gagctggacc agaatccgtg
     8281 gaagctcctt ttcgtcggcc ctaccccctg ggcgtgctcc gcgatgaccg ctgaaagcgg
     8341 gctgtgcact tccagtatgc gtacgaactt ctcccgggag agggcctccc gaagtgccgc
     8401 caagcgaatc tcgttaccta cgctggaaat actcacaagc actccctgac gacgatcctg
     8461 cctccggctc cggtgcactc cgagaaccac gctttcctgc gccactttct cgcggatcgc
     8521 gcttcccgct ctcggagttg ggaggcggtg acaacaaaag cggtgatcgt ctggcgcgtc
     8581 aatgatctcc tcttcgcgca cccgaccggt ggaatacttt gcgccaccgg ccgaacctgc
     8641 cggggtcacg cggagtcgcg gactttaccc gaattcggga actctcttac cttccctata
     8701 catctagatg gttgatcaat tgacgtaacg ctacgttcac cttcccgccc ccacctctac
     8761 gtgtccgggc accgggctcc acatggccgg attcgcacgt cgaacgtgtc cgcgtggcgc
     8821 gaggcgcagg ggtgaacacg gcactccctc gcgcggttcc gcagacgcgg gccggctctg
     8881 ctggctaaaa ttccttgctg tgtaggggag ttgagtatcg ctgcgccttg ttgcggcgac
     8941 atcggggggc gcggtgacag aaccactgag ggttacgccg tcggacggct gcgagatcga
     9001 tgactggagt gccgtcaaag acgcgctgct cggggtggtc cgccacgcgg atcccgcggc
     9061 gggcgagagc acctgccgtg ccgaggggaa gtggcatgct tccgtcgcga gcgtcgaggt
     9121 ggtcaagcgg ctggccggtg gccgtggcgg cgcgcaggtt ctggagatca tcacccgtcg
     9181 ggacggcaac cggcagccca cgctccatgt ggcgaagctg atggacgtgg gtgaggccgt
     9241 caaggagtgg aaggggtaca ggcggctcat cgccacctgt gacagcaaca tgttcgtggc
     9301 tgtcacggcg gtcagccagg gggtcgccga agagcgtacg tgggaggccc gcaagagcgt
     9361 cgtggtgtac cggcacgcca tggactggga tcaccgtgca gaggtgagca tggtggccct
     9421 ggaggacgtg atcgccgccg ccgtggacgg gcacacccct gaggatgagt gtgccgggct
     9481 tgtgcggcag gtgctcgcct cgctggcgga cagcatgtat gcccgcgccg agacgcagga
     9541 aaggcagctg gcggcgtctt tcaaccgttc tcttggcttt gatctctgcc tgtcggtggg
     9601 cagagtcgag gggcgcgccg gtgacttgct cctgatggac ggcaacgccg atgccgagga
     9661 caaggaatca cggcggatct actccgatga cgtggcggag gaatccaccg cgacgcccgg
     9721 tgcgaaacgt tccctgactc ccggccagac ggtgcggctg tccctgagca atcttgaggt
     9781 gagtgacgac gaggtcagag ggacactggt cgagagtgcg gtccatgtgc gggtgaagct
     9841 ggtgggctcc gcgaaggcgg ccgcgagtct gaagcgcatc cgtcaggagc aggcgaaccg
     9901 cagatccgtg gaggtgctcg gtctcatcac ggccgtgcgc gcgcagtcgt ggtcgacagc
     9961 gatggctcgc gcgttcggac cggaggcctt caccgagacg gaggcggcga tcacctacgg
    10021 ggagacgcgt atcgcgcatc cggtgggacg cctggcatcg gtcctgtggg ggcctgccga
    10081 caagcggtcg ttctcgccgg tccaccgcga cctcaatccg cgcaacatca tcactctggg
    10141 cacgactccc tatctgatcg atttcgcgac gttcgaggag tcgggggcga ctctccagga
    10201 tccggcgtgg ctggagacct gcttggtccg ggactgtctc gcggaccggc tgacgtggga
    10261 cgagttggtg cgtcagcagc gtctcctggg cttcctggtg cggatgaccg cgcactggga
    10321 cggcgatcgc gtccgcgcgg cggcggagca ggtcgcccag gccttgacga ccgacaaccc
    10381 acgcctggga cggagcctgc tgctcctgtg gcaggtgcgc ggcacgctca ccgagacctc
    10441 gctgcgggcc gttctcggtg attggcgtgt gcactatcca cagcacttgg ttctggccgc
    10501 ctgcaagacc ctgaagtggg cggagaacgc ggatgaggtg gccaaccccc atcgtttgcg
    10561 ggcgtcgtgc gccgtggccg gggtggcgag tgagagtctg ggaccgctcg gcgagatgtt
    10621 cgccgactgg gcaccggggg acgcggctct cgcctgcgag gtcctgctgg atgccggtga
    10681 ggcgatttcc tcgggcgcgg gagatctgct ccttgcggtg tcctcccggg tggaggaccg
    10741 cgcgttgctg gcccgtgtgg cacagggact gcggagactg ccgtcatcag ccctgtcgga
    10801 ggcgcgccag gcgttactcc ttcgccacct gaaggggccg gatgccccag cgggaaaagc
    10861 agagaggcct gacccgagca cggggcaggc cgatgatctc tcgttcacct acatcgctct
    10921 ggagggccac cggttgccgc cgggggcccc gtgtgtgcag caagggcacg gcgcgttgtc
    10981 ggccgcgtcc gaggactgtg tcgccttggt cgagcgtgaa cagcatgtgg tgctgttgtc
    11041 cgatccggga gcgggaaaga cgacggtggc acgtgagttg catgtgcggc tgctgtcgga
    11101 cgacgtggtc cccgtcagcg gcccactgct gccgctgtgg gtctctgccg tcggtgtcat
    11161 cgagttcttg cgcgcctcca aagagccgac gaccgtccac cggttcctgc gcgtggccaa
    11221 tggtctgcag aaggtgctgt cggacgcgtg tctggcggcg ttgatcgcca tgggcggggt
    11281 gcatgtcacc gttgacgacc tggacctggt cgacagccgc gagcgcagcc agatcatggt
    11341 ctatctggcc cagatagccc gcaaggctcc gaggctcaga ctccttgtgt gcgaccgcat
    11401 ccgcgactac gacccgatgg tcctgcggtg gcccgcggtc gccgtgcaca aggtccgtga
    11461 gggaccggcc cgggacttct tgcgggcggt gctgcgcaag cgggacaagc acggctggaa
    11521 gaaccggttc aagaaccttg aggcccgcct gttccgggac gtcggcgccg gggcgctgcg
    11581 cgatctggcc ggcaagccgc agttcctgaa catgctggtc gaccagtacg cgggcaccga
    11641 cgtactgccg tccaaccccg gcgagttggt gcaccgctat ctcacccggc tgctcaacga
    11701 ctccgcatcg agcgtgcacg tcgatgatct gatccggctg ctgggagcga tcgccaagaa
    11761 gctcgacgcg tccggcgcgc tgcgcaaggc ggcggccgag gccgcgctgg agaaggtggc
    11821 cgagggacag ggccaggagc ggttacggga actgctggcc acgccctgtc tggacgaggt
    11881 cgccggacgg gtcagcttcc gtgatcccct ggtgcagtcc tactgcggtg cggtcgctct
    11941 gcagcggtac ccgaaagagg ctctcaggac cgtcacggac tacgtcctgc ggtacgggtg
    12001 gagagaagcg gccgtcttgc tggtcaccga tccggagacg gcggagacga cgaccgaggc
    12061 cgtcgtgcgg gcgggggtca tcgccagccc ctggtacggg gctctgctga tgcaggccgc
    12121 cccggacagt gcggtcatcg aaatcatccg tgacacgttc ctgcgggagc agaaggacgt
    12181 tctccgctcc tcggacagcg gcgtccctgc ctggaaacgc tccgcgtacg ccctggccaa
    12241 gtacggggac cgggcggctc tggagatcct gcgcgacacc gctctggccg cggaggtatc
    12301 caccgacgcc gcggaagccg ccctggacgg cctggtgatg atgtaccggt ggttcgtccc
    12361 cgatgcgaac acctacctgc aagaggtgct gaccgctttc ctcgacgccc cggccgagcg
    12421 aacaccccgg catccgcggc tggtcacgcg ggctctgcgc agtatccagg tcgcggagct
    12481 gcacagcctc gtgggcctgg cctgggaccg gatcgccgcc catgagccct gggaggtgca
    12541 ctcccaggcg tgggagacgg tgacgcatct gggggtgcgg ccggaccggt ccagaacccg
    12601 tgcctacgcg gacgcctgcg gtgagcggct cctgcgactc gacgcggaac tcaaggagac
    12661 ggccgccacg gagaccacgc agctgctgga ctcggagcgg ctgcggctgc tcacgcacct
    12721 ggcgacgtgc ggtcggctgg aaaccgtcct ggcctaccgg ttccggttcg gactcgccga
    12781 ctaccgggac tggccgcagc tcatcgacag ggcggccgca gcgcagcact ccttccagtg
    12841 gcacggggcg gcggccccca ccttgctgct gacctcggat gtcctgcggg gcatcaacat
    12901 ggcgtccgag cagtggaaga ggatgctgtc gctgggagac gactcactga ccgcactggc
    12961 cgcgcaccat ctcctcgcgg gcaacgtcga cgtcgacgag gcctgtctgc gggacatggt
    13021 ctcgacaggt accagcaagg cgctcctgat cgcgtccgcg ttcgtgcaca gtcttcccca
    13081 gcagatgcac agcatcctgc ccgcactgct cgccccctac ctcgacgaac tggacaccga
    13141 gtcgttggag gcggttgccg cgctggtcag cgccaccgag cacctgcacg ccgagacggg
    13201 acggcaactg gcctggcgtg tccaccgcac cttgctggca cagaatctgg accgggcggc
    13261 cctccactgg ccctggtcgg tcacatggcg gcgggccctg cttccccggg cggagatcgg
    13321 cgacttcctc acggcgcggg cggagcagag cgagcagacc ggcccggccg gcccggccgg
    13381 tcaggacggt gccgagggcg cccgcgaccg cgaactcctg acgctgctgg gcagtg
//
