LOCUS       NZ_CP029618            12934 bp    DNA     linear   CON 19-MAY-2024
DEFINITION  Streptomyces sp. WAC 06738 chromosome, complete genome.
ACCESSION   NZ_CP029618
VERSION     NZ_CP029618.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. WAC 06738
  ORGANISM  Streptomyces sp. WAC 06738
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8324535)
  AUTHORS   Waglechner,N. and Wright,G.D.
  TITLE     Evolution of GPA BGCs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8324535)
  AUTHORS   Waglechner,N. and Wright,G.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2018) Michael G DeGroote Institute for Infectious
            Disease Research, McMaster University, 1280 Main St West, Hamilton,
            Ontario L8S 4L8, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. Jun-2017
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 148.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003945505.1-RS_2024_05_19
            Annotation Date                   :: 05/19/2024 05:01:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,191
            CDSs (total)                      :: 7,115
            Genes (coding)                    :: 6,917
            CDSs (with protein)               :: 6,917
            Genes (RNA)                       :: 76
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 198
            CDSs (without protein)            :: 198
            Pseudo Genes (ambiguous residues) :: 0 of 198
            Pseudo Genes (frameshifted)       :: 107 of 198
            Pseudo Genes (incomplete)         :: 115 of 198
            Pseudo Genes (internal stop)      :: 5 of 198
            Pseudo Genes (multiple problems)  :: 27 of 198
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:28:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6640150
            Orig. end                         :: 6653084
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029618.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Gerard D Wright, McMaster
            University, 1280 Main St West, Hamilton ON, Canada.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..12934
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="18"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12934
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12934
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7934](-)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7934)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     misc_feature    complement(247..261)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.35"
                     /tool="antismash"
     misc_feature    438..454
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 15.31"
                     /tool="antismash"
     gene            632..1573
                     /locus_tag="DMB38_RS29030"
                     /old_locus_tag="DMB38_29020"
     CDS             632..1573
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018843271.1"
                     /locus_tag="DMB38_RS29030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_29020"
                     /product="aryldialkylphosphatase"
                     /protein_id="WP_125937627.1"
                     /transl_table=11
                     /translation="MSVRTVLGDLPADRLGRTDYHEHLFQVSPLLPGDELDDEARSGQE
                     AAYLRAAGFTAMIEATPVGLGRDPAGVARIARATGLHVVHTTGAHREPHYAPGHPLLAL
                     TEAELAGLFTTELTDGMYADADLTVRTGPALRAGIVKAGAGYWSLTPFEHRVLAAAGAA
                     HRATGAPVMVHLEFGSAAFEVLAELAGHGVPAHRVVLAHADRNPDPGLHAELCAAGAYL
                     GYDGMARHRSHPDSTVLGCLLATAERAGPGRLLLGGDVARRSRYRAYGGMPGLDYLPRR
                     FLPRVVAEGGEALADAVTVANPARLLDWSGAP"
     gene            complement(1614..3443)
                     /gene="recQ"
                     /locus_tag="DMB38_RS29035"
                     /old_locus_tag="DMB38_29025"
     CDS             complement(1614..3443)
                     /EC_number="5.6.2.4"
                     /GO_function="GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /gene="recQ"
                     /gene_functions="other (smcogs) SMCOG1122: ATP-dependent
                     RNA helicase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018839552.1"
                     /locus_tag="DMB38_RS29035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_29025"
                     /product="DNA helicase RecQ"
                     /protein_id="WP_125937628.1"
                     /transl_table=11
                     /translation="MSSTEALQVLSKVFGYESFREGQQEIIDHVVGGGDALVLMPTGGG
                     KSLCYQIPALVRPGTGVVISPLIALMQDQVDALRALGVRAGFLNSTQDPDERRMVEAEF
                     LAGEIDLLYLAPERLRVESTMRLLDRGTVALFAIDEAHCVAQWGHDFRPDYLALSALSE
                     RWPDVPRIALTATATKATHAEIASRLQLEQARHFVASFDRPNIQYRIVPKREPKKQLLE
                     LLRTEHAGDAGIVYCLSRKSVEDTAAFLVQNGIDALPYHAGLPAATRAEHQSRFLREEG
                     MAIVATIAFGMGIDKPDVRYVAHLDLPKSVEGYYQETGRAGRDGQPSTAWLAYGLQDVV
                     QQRKLIDGSEGDDAHRRRASAHLDAMLALCESVDCRRSQLLAYFGQSGSPCGNCDTCLT
                     PPESWDGTVAAQKLLSTVVRLRRERRQKFGAGQIVDILMGRKTAKVIQHDHDTLSVFGV
                     GDDLAEHEWRGVVRQLLAKGLLAVEGDYGTLVLTEEADEVLYRGRKVPLRTEPKRAAKA
                     AKAKSGSKRAEAVALPAEAASLFERLRAWRGATAKEQGVPAYVIFHDATLRQIATERPG
                     SLASLGSVSGVGENKLAKYGEAILQTVAEDDAG"
     gene            complement(3531..3803)
                     /locus_tag="DMB38_RS29040"
                     /old_locus_tag="DMB38_29030"
     CDS             complement(3531..3803)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010043586.1"
                     /locus_tag="DMB38_RS29040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_29030"
                     /product="hypothetical protein"
                     /protein_id="WP_240662519.1"
                     /transl_table=11
                     /translation="MARRWKLGAAAMAAGTALLVAGCTDPPTDSSEIVTFTDGHGRVCT
                     GSVVVDREQSEGTDYEITGLDCEYPPEGRSPGPDSYRPLPQRESD"
     misc_feature    3798..3808
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     gene            complement(3900..4904)
                     /locus_tag="DMB38_RS29045"
                     /old_locus_tag="DMB38_29035"
     CDS             complement(3900..4904)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018843268.1"
                     /locus_tag="DMB38_RS29045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_29035"
                     /product="proline racemase family protein"
                     /protein_id="WP_125937629.1"
                     /transl_table=11
                     /translation="MRASRYFTAVDSHTEGMPTRVITGGVGVVPGATMAERRAYFAEHL
                     DDIRRLLVNEPRGHAAMSGAILQPPTRPDADYGVLYIEVSGLLPMCGHGTIGVASVLVE
                     TGMVTVTEPVTEVRLDTPAGLVTARVAVSGGKAESVTFRNVPSFALELDAAVEVPGLGR
                     VTYDIAFGGNFYAILPIERLGIPFAKAEKERMLTAGLAIMDAINERNRPVHPADPGISG
                     CKHVQLTAPPAGGPADARNAMVIHPGWFDRSPCGTGTSARMAQLYARGELELGADFVNE
                     SLLGTLFTGRLEAGTEIAGLPAQIPTVTGRAWITGMSQYLLDPDDPFPAGFTL"
     gene            complement(5001..6365)
                     /locus_tag="DMB38_RS29050"
                     /old_locus_tag="DMB38_29040"
     CDS             complement(5001..6365)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018854413.1"
                     /locus_tag="DMB38_RS29050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_29040"
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_125937630.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.6e-26, bitscore:
                     92.0, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 6.9e-09, bitscore:
                     34.7, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGPGAEGPVVVIGGGPAGMAAARAAARHGVRVLLVDSGAALGGQ
                     YHRQNSLTGHDRFALPPGVEHLPHTVVWALEPVPEGGHRVHLRTGPADGPGRTARSVAT
                     RALVLATGAYDRALPFPGWDLPGVYTAGAAQALAKGQGVRVGERVLVAGTGPFLLPVAA
                     SLTAAGARVAGVLEAAEPVTAWLRRPADAVAGWRKSGELAGYGALLARHRIPYRRRTAV
                     VAAHGTDRVEAVTTARLTPGWEIVPGSERRVEADAVCVGFGFVPQVETALAAGCALAGG
                     FVAVDAAQATSVPGVFAAGELTGVGGAGPAAAEGEVAGVAAAKLLGARPAPPAAALRRV
                     RAGRGFAAALARAYPVPDGWRTWLADDTLVCRCEEVPYGELRRAVREREALGVRAVKLV
                     SRAGLGQCQGRVCGSSVACLTGVVDAAAFARRPISSPVRLGELAEPPPADPEPGT"
     gene            complement(6362..6619)
                     /locus_tag="DMB38_RS29055"
                     /old_locus_tag="DMB38_29045"
     CDS             complement(6362..6619)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018839558.1"
                     /locus_tag="DMB38_RS29055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_29045"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_164716135.1"
                     /sec_met_domain="Fer2_4 (E-value: 8.7e-20, bitscore: 69.3,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVRVDGEELVTEAGRTVAGLLLALGRVSWRTTRGGGRPRGVFCG
                     IGVCHDCLVVVNGAADVRACQRLVRDGDDIRTQDGAELPA"
     gene            complement(6738..7934)
                     /locus_tag="DMB38_RS29060"
                     /old_locus_tag="DMB38_29050"
     CDS             complement(6738..7934)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018839559.1"
                     /locus_tag="DMB38_RS29060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_29050"
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_125939346.1"
                     /sec_met_domain="DAO (E-value: 2.4e-61, bitscore: 207.0,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAYDVVVVGAGVIGAACAEALTAAGLSVAVLDHGAPASGTTAAGE
                     GNVLVSDKAPGPELALAQHSRRRWPRLLAELRDLLGPRRAEAEWEAKGGLVVATAPAAA
                     SALADFAAAQRTAGVRAEPVTPAEAGALEPHLTADVTAAVHYPQDGQLQPVLATTALLA
                     AVRERGGRVRAGVTAHGVETGAGGAVTGVRTDRGTVPCGAVVNACGPWAGRFAAAAGAP
                     VAVLPRRGLVLVTAAVPAGTVRHKVYDADYVGAVAGGTAALQTSTVVEATRAGTVLVGS
                     SRERAGFDETVRVAVLRELARKAAGLFPVLAGVPVIRAYGGFRPYVPDHLPVIGEDPRR
                     PRLWHATGHEGAGIGLAAGTGQLLADLFTGRAPEVPAEPFRVDRPGVMTEAEAADEPA"
     misc_feature    complement(7955..7969)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.9"
                     /tool="antismash"
     gene            complement(7999..8634)
                     /locus_tag="DMB38_RS29065"
                     /old_locus_tag="DMB38_29055"
     CDS             complement(7999..8634)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1148: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018854415.1"
                     /locus_tag="DMB38_RS29065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_29055"
                     /product="XRE family transcriptional regulator"
                     /protein_id="WP_125937632.1"
                     /transl_table=11
                     /translation="MVREQAGRVLGANLRALRARAGLSLSGLARASGIAKGTLSQLESG
                     TGNPTIETVFSLSNALGVPVSSLLTEREDPDVLLVRSAGLDVLSSNAVDLRMLRRMDLT
                     DTVVELYDQRVRPGEVQHSAGHPGREHVVVTSGRLRVGPPEAPYELGPGDYVCFPAAAP
                     HVYETVGGPVLSVLMLEYPAAAHPVPPVASCAVPAPRQNAPRLPGTGA"
     misc_feature    8709..8723
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 15.86"
                     /tool="antismash"
     misc_feature    complement(8710..8725)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.11"
                     /tool="antismash"
     misc_feature    8779..8796
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.44"
                     /tool="antismash"
     gene            8897..10003
                     /locus_tag="DMB38_RS29070"
                     /old_locus_tag="DMB38_29060"
     CDS             8897..10003
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018839561.1"
                     /locus_tag="DMB38_RS29070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_29060"
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="WP_125937633.1"
                     /transl_table=11
                     /translation="MAAHPGFDRRALLRGGLTASAAGALGLGAAGLAHAAPSSATLAAP
                     GAARGARALAAPEPRIYGTAEWGARPPATDIVILDHVPTYIAVHHTAEPGNSEDYSLEH
                     AMEICRSIQNFHMDTQGWGDTGQQFTISRGGYCLEGRHESLGVVRGGTQHVQGANVGGR
                     NSEVIGIENEGLYTDVDVPTALWDSLVAMVAWIAGQYGRPVENIQGHRDFNSTECPGDV
                     LYARLPELRDAVAGVLGAPKPERIAVWPLLRPGDSGPVVRAAQHLLRARGFTGLPADGL
                     FGPETKQAVATLAEEGGIERHSCSAEFHKRNDETGYLGADIWPMITPRGRAGAGGEVAK
                     AVEALLGRGRRAPAGRELDATVWKQLLS"
     gene            complement(10072..10752)
                     /locus_tag="DMB38_RS29075"
                     /old_locus_tag="DMB38_29065"
     CDS             complement(10072..10752)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020679070.1"
                     /locus_tag="DMB38_RS29075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_29065"
                     /product="hypothetical protein"
                     /protein_id="WP_240662520.1"
                     /transl_table=11
                     /translation="MGRVRRGVAGLVLVAAAGLAGTAACTGGDDGGDAKGRKEADPTAS
                     PDPLPRTPPPARQTVASSEDACDFTGPFEAGKVKPYLPGGPAYEGPGPHPAVLFKDDIA
                     VDESEPPQLPGDWTPDFANTDTQLVVCQYDDDSHDSRTVGTCTYLGGSHNTGDGVVDVQ
                     SARYVFRVFEAATGEPVTKFALDGTTSPQQTCPDSALYPATDFQLVTSEALADRLRPFV
                     AGRR"
     misc_feature    complement(12194..12208)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.35"
                     /tool="antismash"
ORIGIN
        1 gcggcgaggt gtcctccgtc tccaagcggg acggccgctt catcggccac gccaaggacc
       61 cgctcaccgc gggcctggcc atcgaggagt tcctcgcgcc cggccacttc gccgccaccg
      121 gcggcgagtt gctctgcctg ggcgccggcg gcgccggcac cgcgatcagc tactacctcg
      181 cccgccgccc cgacgccccc gcccgcatcg tctgcaccga ccgctccgcc ggccggctcg
      241 ccgagctggc cgacgtgctc cggcgcggcg gcgcggaccc cgcccggctg cgtaccgtcg
      301 tcgccgaggg ccacgccgac gacctgctcg cggcggcccc gcccggcacc ctcgtcgtca
      361 acgccaccgg tctcggcaag gaccggccgg gatcaccgct gtcgcccgcc gcgcggttcc
      421 cgcgcggggc cgtggcgtgg gagctgaact accgcggcgc gctgcccttc ctgcacgcgg
      481 cccgcgccca ggccgccgaa cgggacctcg acgtcgtcga cggctggcgg tacttcatcc
      541 acggctggtc ccaggtgata gcggaggtgt tcggcctcac cctggacgcc gcgaccgtcg
      601 accggctctc cgccgccgcc gaggccgtac ggtgagcgtc cgcaccgtcc tcggcgacct
      661 gcccgcggac cggctcgggc gcaccgacta ccacgagcac ctcttccagg tctcgccgct
      721 gctgcccggc gacgagctgg acgacgaggc gcgcagcggg caggaggccg cctacctgcg
      781 ggcggccggc ttcacggcga tgatcgaggc gaccccggtg ggcctgggcc gcgacccggc
      841 gggcgtcgcc cgcatcgcgc gcgccaccgg tctgcacgtc gtgcacacca ccggcgccca
      901 ccgcgagccg cactacgcgc cgggacaccc gctgctcgcg ctcaccgaag ccgaactggc
      961 cggcctcttc accaccgagc tcaccgacgg catgtacgcg gacgcggacc tgacggtacg
     1021 gaccgggccg gccctgcgcg ccgggatcgt caaggcgggc gccgggtact ggtcgctcac
     1081 cccgttcgag caccgggtgc tcgcggcggc cggcgccgcg caccgggcca ccggggcgcc
     1141 ggtgatggtc catctggagt tcggcagcgc ggcgttcgag gtgctggcgg agctggccgg
     1201 gcacggggtg ccggcgcacc gggtcgtcct ggcgcacgcc gaccgcaacc ccgaccccgg
     1261 gctgcacgcg gagctctgcg cggccggcgc gtacctcggc tacgacggca tggcccggca
     1321 ccgcagccac cccgactcca ccgtgctcgg ctgcctgctc gccaccgcgg agcgcgcggg
     1381 tcccggccgg ctgctgctcg gcggcgacgt cgcgcgccgc agccgctacc gcgcgtacgg
     1441 cggcatgccg ggcctggact acctgccccg ccgcttcctc ccgcgcgtcg tggccgaggg
     1501 cggcgaggcg ctggccgatg ccgtcacggt ggccaacccg gcgaggctgc tggactggag
     1561 cggcgcgccc tgaggcgagg gcccgccctc acccgcgccc caccccgcgg ccctcatccc
     1621 gcgtcgtcct ccgcgacggt ctggaggatc gcctcgccgt acttcgcgag cttgttctcg
     1681 ccgacgccgc tgacggaacc cagcgaggcg agcgagccgg ggcgctcggt ggcgatctgc
     1741 cgcagcgtcg cgtcgtggaa gatcacgtac gcgggcaccc cctgctcctt cgccgtcgca
     1801 ccccgccacg cgcgcagccg ctcgaagagc gacgccgcct ccgccggcag ggcgacggcc
     1861 tcggcgcgct tggacccgga cttcgccttc gccgccttcg ccgcccgctt gggctcggtg
     1921 cgcagcggca ccttgcggcc ccggtagagc acctcgtcgg cttcctcggt gagcaccagc
     1981 gtgccgtagt cgccctcgac ggccagcagc cccttcgcga gcaactgccg tacgacaccg
     2041 cgccactcgt gctccgcgag gtcgtcgccg acgccgaaga ccgagagcgt gtcatggtcg
     2101 tgctggatga ccttggccgt cttgcggccc atgaggatgt cgacgatctg cccggcgccg
     2161 aacttctgcc gccgctcgcg ccgcagccgc accaccgtgg agagcagctt ctgcgcggcg
     2221 accgtgccgt cccaggactc ggggggcgtg aggcacgtgt cgcagttgcc gcagggggag
     2281 ccgctctggc cgaagtacgc gagcaactgc gagcgccggc agtcgacgct ctcgcagagc
     2341 gcgagcatcg cgtcgaggtg cgcgctcgcc cgccgccggt gcgcgtcgtc gccctcggag
     2401 ccgtcgatga gcttgcgctg ctgcaccacg tcctggagcc cgtacgcgag ccacgcggtg
     2461 gacggctggc cgtcgcgtcc tgcgcggccg gtctcctggt agtacccctc gacggacttg
     2521 gggaggtcga gatgggcgac gtagcgcacg tccggcttgt cgatgcccat gccgaacgcg
     2581 atggtggcga cgatcgccat gccctcctcg cgcaggaacc gcgactggtg ctcggcgcgg
     2641 gtcgccgcgg gcagccccgc atggtagggc agcgcgtcga tcccgttctg cacgaggaag
     2701 gccgcggtgt cctccacgga cttgcgcgac aggcagtaga cgatgccggc gtcgcccgcg
     2761 tgctcggtgc gcagcagctc caggagctgc ttcttcggtt cccgcttggg cacgatgcgg
     2821 tactggatgt tcggccggtc gaagctggcc acgaagtgcc gcgcctgctc cagttgcagc
     2881 cggctcgcga tctccgcgtg cgtcgccttg gtcgcggtcg cggtgagcgc gatgcgcggc
     2941 acgtccggcc agcgctcgga gagcgccgac agcgcgaggt agtcgggccg gaagtcgtgc
     3001 ccccactgcg cgacgcagtg cgcctcgtcg atggcgaaca gcgcgacggt cccgcggtcg
     3061 agcagccgca tcgtggactc cacccgcagc cgctcgggcg ccagatagag caggtcgatc
     3121 tccccggcga ggaactcggc ctccaccatc cgccgctcgt cgggatcctg cgtggagttg
     3181 agaaacccgg cccgcacgcc gagggcgcgc agcgcgtcga cctggtcctg catgagcgcg
     3241 atgagcggcg agatcaccac tccggtgccg ggcctgacca gcgcgggaat ctgatagcac
     3301 agggacttgc cgccgccggt gggcatcagc acgagggcgt cgccgccgcc cacgacatgg
     3361 tcgatgatct cctgctgccc ctcgcggaac gactcgtagc cgaagacctt gctgaggacc
     3421 tgcagcgcct cggtggaact catcgggcga gtttatgccc cgccaccgac accccgcggg
     3481 gagacctccg gaccggccgg gcagcgcggc cggtccggtc gggtcctttg ctagtcggac
     3541 tcccgctgcg gcagcgggcg gtacgagtcg gggcccgggc tccggccctc gggcgggtac
     3601 tcgcagtcga ggccggtgat ctcgtagtcg gtgccctcgc tctgctcccg gtccacgacg
     3661 acggagcccg tgcacacgcg gccgtgtccg tcggtgaagg tgacgatctc gctggagtcg
     3721 gtcggggggt cggtgcaccc cgcgaccagc agtgccgtgc ccgcggccat cgccgccgcg
     3781 ccgagcttcc accgccgtgc catgccacgc tcctccgcgt cggtgcaccc ggcggtccgg
     3841 gcgccaccac cggcaccgta ggacggagcg cccgggagcc ctcccgcggg ggcccggaac
     3901 tacagcgtga agccggcggg gaacgggtcg tcggggtcca ggaggtactg ggacatgccg
     3961 gtgatccagg cgcggcccgt caccgtgggg atctgggccg gcaggccggc gatctccgtc
     4021 ccggcctcca ggcgtcccgt gaacagcgtg ccgagcagcg actcgttgac gaagtccgcg
     4081 cccagctcca gctccccgcg cgcgtacaac tgcgccatcc gcgccgacgt gcccgtgccg
     4141 cagggggagc ggtcgaacca gcccgggtgg atgaccatcg cgttgcgcgc gtccgccggg
     4201 ccgcccgcgg gcggcgccgt caactggacg tgcttgcagc ccgagatccc cgggtccgcc
     4261 gggtgcaccg gccggttccg ctcgttgatc gcgtccatga tcgccaggcc cgcggtgagc
     4321 atccgctcct tctccgcctt cgcgaacgga atgcccaggc gctcgatcgg caggatggcg
     4381 tagaagttgc ccccgaacgc aatgtcgtac gtgacccgcc ccaggcccgg cacctccacc
     4441 gccgcgtcca gctccagcgc gaacgacggc acgttgcgga acgtcaccga ctccgccttg
     4501 ccgcccgaga ccgcgacccg ggcggtcacc agccccgcgg gcgtgtcgag acggacctcc
     4561 gtgaccggct cggtgaccgt gaccatcccg gtctcgacca gcaccgaggc caccccgatc
     4621 gtgccgtgcc cgcacatcgg cagcagcccg gagacctcga tgtagagcac gccgtagtcg
     4681 gcgtccggcc gggtcggcgg ctggaggatc gcgccgctca tcgcggcgtg cccgcgcggc
     4741 tcgttcacca gcagtcgccg gatgtcgtcc aggtgctcgg cgaagtacgc gcggcgctcg
     4801 gccatcgtgg cgcccggcac caccccgacg ccgccggtga tcacccgggt cggcatgccc
     4861 tcggtgtgcg agtcgacggc cgtgaagtag cgggaggcgc gcatgtcaca cccccgccgg
     4921 gtgggccggg acggctctgt ccagggtgtc gtgcggcatg gcggtctcct tggaaggacg
     4981 gtgtggcggt acggggacgg tcacgttccg ggctccgggt ccgcgggcgg cggctcggcc
     5041 agctcgccca gccgtaccgg cgacgagatc gggcggcgcg cgaacgccgc cgcgtccacc
     5101 acgcccgtca ggcaggcgac gctggagccg cacacgcggc cctggcactg gccgagcccc
     5161 gccctgctga ccagcttcac ggcccgtacc ccgagcgcct cgcgctcccg gaccgcgcgg
     5221 cgcagttcgc cgtacgggac ctcctcgcag cggcacacga gggtgtcgtc ggccagccag
     5281 gtgcgccacc cgtccggcac cgggtacgcc cgcgccagcg cggcggcgaa cccgcgtccg
     5341 gcccgtaccc ggcgcagcgc cgccgccggg ggcgccggac gggcgccgag gagcttcgcc
     5401 gcggcgacgc ccgcgacctc gccctccgcg gcggccgggc cggcgccgcc gacgcccgtc
     5461 agctcgcccg ccgcgaacac ccccggcacc gaggtggcct gcgccgcgtc gaccgcgacg
     5521 aacccgccgg cgagcgcgca gcccgcggcg agagcggtct ccacctgcgg gacgaatccg
     5581 aagccgacgc acaccgcgtc ggcctccacc cgccgctcgc tgcccggcac gatctcccag
     5641 ccgggggtga ggcgcgcggt ggtgaccgcc tccacgcggt ccgtgccgtg cgcggcgacg
     5701 acggcggtgc ggcgccggta cgggatgcgg tggcgggcca ggagcgcgcc gtacccggcg
     5761 agttcgccgg acttgcgcca tccggcgacg gcgtcggcgg gccggcgcag ccaggccgtg
     5821 accggctcgg cggcctccag caccccggcc acccgggccc cggccgccgt cagcgacgcg
     5881 gcgaccggca gcaggaaggg accggtgcct gccaccagga cccgctcgcc gacgcgcacg
     5941 ccctgcccct tcgccagcgc ctgcgcggcg cccgcggtgt agacgccggg caggtcccag
     6001 ccggggaacg gcagcgccct gtcgtacgcc ccggtggcca gcaccagcgc gcgggtcgcc
     6061 acgctccgcg cggtacggcc ggggccgtcg gccgggccgg tgcgcaggtg cacccggtgc
     6121 ccgccttccg gcacgggctc cagcgcccag acgacggtgt gcggcagatg ctcgacgccg
     6181 ggcggcagcg cgaaccggtc gtggcccgta agggagttct gccggtggta ctggccgccg
     6241 agcgccgcgc cggagtccac gagcaggacg cggacgccgt gccgggccgc ggccctggcg
     6301 gcggccatcc cggcgggccc gccgccgatg acgacgacgg gtccttccgc cccgggcccg
     6361 ctcatgccgg cagctccgcg ccgtcctggg tgcggatgtc gtcgccgtcg cggacgaggc
     6421 gctggcaggc gcggacgtcg gcggctccgt tgaccaccac gaggcagtcg tggcagacgc
     6481 cgatgccgca gaagacgccg cgcggccggc cgccgccgcg ggtcgtgcgc caggacaccc
     6541 ggccgagcgc cagcagcagg ccggcgacgg tccgccccgc ctcggtgacc agctcctccc
     6601 cgtcgacccg cacgctcaac gggtccccgc cccgccccgc cggggccgcg cccggacgcc
     6661 gctcaccggc ccccgccgcc cggtccggca acgggcctcc cgccccggtc cggccgccgc
     6721 gccacgcccg cccccgccta cgccggttca tccgccgcct ccgcctccgt catgactccc
     6781 ggccggtcca cccggaacgg ctccgcgggc acctccggcg cccgccccgt gaacaggtcc
     6841 gccagcaact gcccggtgcc cgccgccagc ccgatgcccg cgccctcgtg gccggtggcg
     6901 tgccagagcc gggggcggcg cgggtcctcg ccgatcaccg gcaggtggtc ggggacgtac
     6961 ggccggaagc cgccgtacgc gcggatcacc gggactccgg cgagcacggg gaagagcccg
     7021 gccgccttgc gcgccagctc gcgcagcacc gccacccgca ccgtctcgtc gaagccggcc
     7081 cgctcccggc tggagccgac gagcaccgtc ccggcccgtg tggcctccac gacggtggac
     7141 gtctgcaggg ccgcggtgcc gccggcgacc gcgccgacgt agtcggcgtc gtagaccttg
     7201 tgccgcaccg tgcccgccgg caccgcggcg gtgacgagga cgagaccgcg ccgcggcagc
     7261 accgcgaccg gcgcccccgc cgccgcggcg aaccggccgg cccacggccc gcaggcgttg
     7321 acgaccgcgc cgcacggcac cgtgccccgg tcggtgcgca cgccggtgac ggcgcccccg
     7381 gcgccggtct cgaccccgtg cgcggtgacg ccggcgcgca cccgcccgcc ccgctcccgt
     7441 accgccgcca gcagcgcggt cgtcgcgagc accggctgca actggccgtc ctgcgggtag
     7501 tggacggccg ccgtgacgtc cgcggtcagg tgcggctcca gcgccccggc ctccgcgggg
     7561 gtcaccggct ccgcccggac gcccgcggtg cgctgggccg cggcgaagtc cgcgagcgcg
     7621 ctcgccgccg cgggcgcggt ggcgacgacg aggccgccct tggcctccca ctccgcctcc
     7681 gcgcggcgcg ggccgagcag gtcgcgcagt tccgcgagca accggggcca gcggcggcgg
     7741 gagtgctggg cgagggccag ttcgggtccg ggcgccttgt cggagaccag gacgttgccc
     7801 tcgcccgcgg cggtggtgcc gctcgcgggg gcgccgtggt cgagcacggc caccgagagc
     7861 ccggcggcgg tcagcgcctc ggcgcaggcc gcgccgatca ctccggcgcc cacgaccacg
     7921 acgtcgtacg ccacctgccg cccacctccg ttcattaaaa cgaacgtcaa cgagcgtatg
     7981 gagggcgccg atcggcggtc aagcccctgt tccggggagg cgaggtgcgt tctgtcgcgg
     8041 cgccggcacg gcgcacgagg cgaccggcgg gaccgggtgc gcggcggccg ggtactccag
     8101 catcagcacc gagagcaccg ggcccccgac cgtctcgtag acgtgcgggg cggcggccgg
     8161 gaagcagacg tagtcgccgg ggcccagctc gtacggcgcc tccggcgggc ccacccggag
     8221 ccgcccggag gtgacgacga cgtgctcgcg cccggggtgg cccgccgagt gctgcacctc
     8281 gcccgggcgc acccgctggt cgtacagctc gacgaccgtg tcggtgagat ccatccggcg
     8341 cagcatccgc aggtcgaccg cgttgctgct gagcacgtcg aggccggccg agcgcaccag
     8401 cagcacgtcg gggtcctcgc gctcggtgag cagcgaggac accggcaccc ccagagcgtt
     8461 cgacaaactg aacaccgtct cgatggtggg gttcccggtc ccggactcca gttgcgacag
     8521 cgtgcccttg gcgatcccgg aggcgcgcgc cagcccggag agggagagcc ccgcccgggc
     8581 gcgcagcgcc cgcaggttgg ccccgagcac ccggcctgcc tgttcgcgta ccaccggacc
     8641 tcctcgacag tcgtgcgaga ggctaccccg cgcccggcga ggcggcgacg agcgtaatgg
     8701 ttattcgcgt atcgtcgttt tcattggtcg gcccattgac gcgggagttg accccaattc
     8761 cccgtggaat aggggccgtt gttactcagg ggttgtcgtg tgcatgtccc ggatgttttc
     8821 atggccgacc ggacagcgcg cgcgcacaat ccttgatccc cccacatcca ccccccacct
     8881 gaagaaggag accaggatgg cagcacatcc gggtttcgac cgccgcgccc tgctgcgcgg
     8941 cggactgacc gcctccgcgg ccggcgcact cggcctcggc gctgcggggc tcgcacacgc
     9001 cgcgcccagc tcagcgaccc tggccgcccc cggcgcagca cgcggcgccc gtgcgctcgc
     9061 ggcgcccgaa ccgcgcatct acggcaccgc ggaatggggt gcccgaccgc cggcgaccga
     9121 catcgtcatc ctcgaccacg tgccgacgta catcgccgtc caccacaccg ctgaaccggg
     9181 caacagcgag gattactcgc tcgaacacgc catggaaatc tgccggtcca tccagaactt
     9241 ccacatggac acccaggggt ggggcgacac cggccagcag ttcaccatca gccgcggcgg
     9301 atactgcctc gaaggccggc acgagagcct gggcgtcgtg cgcggcggca cccagcacgt
     9361 gcagggcgcc aacgtcggcg gccgcaacag cgaggtcatc ggcatcgaga acgagggcct
     9421 ctacaccgac gtcgacgtgc ccaccgcgct gtgggactcg ctcgtcgcga tggtcgcctg
     9481 gatcgccggc cagtacgggc ggccggtcga gaacatccag ggccaccgcg acttcaactc
     9541 caccgagtgc cccggcgacg tgctctacgc ccgcctgccg gagctgcgcg atgcggtggc
     9601 cggggtgctc ggtgccccga agcccgagcg gatcgccgtc tggccgctgc tgcgccccgg
     9661 cgacagcggc cccgtggtgc gcgcggcgca gcacctgctg cgcgcccgcg ggttcaccgg
     9721 gctgcccgcc gacgggctct tcggcccgga gacgaagcag gccgtggcca cgctcgccga
     9781 ggagggcggc atcgagcggc actcctgctc ggcggagttc cacaagcgca acgacgagac
     9841 cggctacctc ggcgcggaca tctggccgat gatcaccccg cgcgggcgcg ccggggccgg
     9901 cggcgaggtc gcgaaggccg tcgaggcgct gctcggccgc gggcggcggg cgccggcggg
     9961 ccgggagctg gacgcgacgg tgtggaagca gttgctgtcc tgagggcctg agcacgcacg
    10021 caacggcacc ggcgaccgcc gcgccccccg ggcccgcgac ggtcgccggt gtcagcggcg
    10081 gcccgccacg aacgggcgga gccggtcggc gagcgcttcg ctcgtcacca actggaagtc
    10141 cgtggccgga tacagggccg agtcggggca cgtctgctgc gggctcgtgg tgccgtccag
    10201 ggcgaacttc gtcaccggct cgccggtggc ggcctcgaag acccggaaga cgtagcgcgc
    10261 gctctgcacg tcgaccacgc cgtcgccggt gttgtgcgag ccgccgagat aggtgcaggt
    10321 gcccacggtg cggctgtcgt ggctgtcgtc gtcgtactgg cagaccacga gctgggtgtc
    10381 ggtgttcgcg aagtccggcg tccagtcgcc cgggagctgg ggcggctcgc tctcgtcgac
    10441 ggcgatgtcg tccttgaaga gtacggccgg atgcggcccg gggccctcgt atgcgggccc
    10501 gccgggaagg tacggcttca ccttgccggc ctcgaacggc ccggtgaagt cgcaggcgtc
    10561 ctcgctgctc gccaccgtct gccgcgccgg cggcggcgta cgcggcagcg ggtcggggga
    10621 agcggtcggg tccgcttcct tccgcccctt cgcgtcgccg ccgtcatcgc ccccggtgca
    10681 ggcggccgtc cccgccagcc cggcggccgc gaccagcacg agcccggcca cgccccgtcg
    10741 cacacgtccc acgatgccgc tcctcccgcc acgatccgcc cgcacgataa tgccccgggc
    10801 ccgggacggg tccccgccac gaggtgattc gcgcgcacct gccgccaagg tcgactacgg
    10861 gggctctcca ggtggttggg taggggtcga gcagtgccgg ggggacgtgc gctccggtgc
    10921 ggaaggagga gtgccgtgga gccgctggcg gctggggatc ccgcccgggt gggcgacttc
    10981 gtgctacgcg gccggctggg cgcgggcggc atgggggagg tgttcctcgg ccggtcgccc
    11041 ggcgggcggg ccgcggcggt gaaggtggtg cacgcccacc tggcgcggca gcccgagttc
    11101 cgcggccggt tccggcggga ggtggccgcc gcgcggtcgg tcagcggcgc gtacaccgcg
    11161 cccgtgatgg ccgcgggccc ggaggacgat ccgccgtgga tcgccacgct ctacgtcccc
    11221 ggcccctccc tggccgaagc cgtgcgggcg gccgggccgt tccccgaggg cgcggtgtgg
    11281 ccgctggccg ccgggctggt cgaggcgctc caggccatcc acgccgaggg gctgctccac
    11341 cgcgacctca cgccgtcgaa cgtgctgctg gccgcggacg ggccgcgggt catcgacttc
    11401 gggatctccc gggccgtgga cggcacggtg ctcaccggga cgggcgcgac catcggcacg
    11461 cccggcttca tgtgcccgga gcaggcccag gacggcgaag tccgccagcc gggcgacgtc
    11521 ttctccttcg gcgccgtact ggcctatgcc gccggcggcg tcgagccgtt cgggcacggc
    11581 gcgccgctgg gagtgctgca ccgcgtggtg acggcggaac cgcacctgga cgaggtgccc
    11641 gaacggatac gcaccctcgc ggcggcgtgc ctggccaaga acccggacga ccggcccacc
    11701 cccgccgaac tcctcggcct catcgccgcg gagtgggacc cgggcgacga cttctccgcc
    11761 gcggcgccgt ggcccgcggc cgtgcacacc ctcatcgagg cccgcgccgt acccggtacg
    11821 gcgcgggcca ccgaacccga cggcgcgccg ccgcctccgc acgccgcgcc cacggccacc
    11881 gcgcccccgc ggtacacccc gacggagccg ggcggccggc cgcaggagcc ggagccggaa
    11941 gccccgcccg cccccgccgc ctccggccgc gtaccgaagg aggaccgccc ccgcgcgctg
    12001 cggcgccgcc accggcaggc gctcaagctc accaggtcgg gccagtacga ggaagccgaa
    12061 cggcagctcg ccgccctcgt cgcggaccgg gcccgggtgc tggggtccga ccatcccgac
    12121 gtgcagttca cccgccagca ccaggcccgg gcgctcgcgg agacgggccg gcacgcggag
    12181 gccgagcggc tgctggccga cgtcctcccg cgcatcctgc agctgcgggg gcccgatcac
    12241 cgggacacgc tcatcacccg ccggcaccac gcggaagcgc tcgggaacgc gggccggccg
    12301 gaggaagccg tgcccctcct cgccgacctc gtcgccgaca tggtgcgcct gcacggcccc
    12361 gaccaccccg acacgctgct cacccgccat ctgcacgcct gcaacctcgc ggacacgggg
    12421 gactacgcgc aggcggcgcg cctcttcgcc gacctcctcg acgaccggat ccgcgtgcac
    12481 ggacgcaacc acgagaagac cctggtcgcc cgcatgctcc acgcccacta cgtcggatcg
    12541 tcgggggagc acgcggaagg cgcccgcctg atgagcgaac tcatccccga cctctcccgg
    12601 gtgcggggcc ccgaccacgc cgagaccctg gcagcccgcg gcagcagggc ccgccatctc
    12661 ggtgaggccg gggaacacgc ggaggccgtg cgtgcgttcg cgggactggc cgccgacacc
    12721 ggtcgcgtcc tgggtcccga ccacgacgac accctgagcg tgcgcgagcg ccacgccact
    12781 tatctcggcg cgtcggggga gcacgcggag gccgcccggc tgcacgccgg actggccgcc
    12841 gaccacgcgc gcctcctggg cccggaccac cgcgacaccc tgctcgcccg ccacctcagc
    12901 gcgtgggagc tgggagaggc gggcgaatac gccg
//
