LOCUS       NZ_CP029618            10798 bp    DNA     linear   CON 19-MAY-2024
DEFINITION  Streptomyces sp. WAC 06738 chromosome, complete genome.
ACCESSION   NZ_CP029618
VERSION     NZ_CP029618.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. WAC 06738
  ORGANISM  Streptomyces sp. WAC 06738
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8324535)
  AUTHORS   Waglechner,N. and Wright,G.D.
  TITLE     Evolution of GPA BGCs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8324535)
  AUTHORS   Waglechner,N. and Wright,G.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2018) Michael G DeGroote Institute for Infectious
            Disease Research, McMaster University, 1280 Main St West, Hamilton,
            Ontario L8S 4L8, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. Jun-2017
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 148.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003945505.1-RS_2024_05_19
            Annotation Date                   :: 05/19/2024 05:01:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,191
            CDSs (total)                      :: 7,115
            Genes (coding)                    :: 6,917
            CDSs (with protein)               :: 6,917
            Genes (RNA)                       :: 76
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 198
            CDSs (without protein)            :: 198
            Pseudo Genes (ambiguous residues) :: 0 of 198
            Pseudo Genes (frameshifted)       :: 107 of 198
            Pseudo Genes (incomplete)         :: 115 of 198
            Pseudo Genes (internal stop)      :: 5 of 198
            Pseudo Genes (multiple problems)  :: 27 of 198
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:28:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2046379
            Orig. end                         :: 2057177
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029618.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Gerard D Wright, McMaster
            University, 1280 Main St West, Hamilton ON, Canada.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10798
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10798
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10798
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5798](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5798)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(226..246)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.01"
                     /tool="antismash"
     gene            273..833
                     /locus_tag="DMB38_RS08605"
                     /old_locus_tag="DMB38_08610"
     CDS             273..833
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1148: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018853994.1"
                     /locus_tag="DMB38_RS08605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_08610"
                     /product="XRE family transcriptional regulator"
                     /protein_id="WP_125934667.1"
                     /transl_table=11
                     /translation="MVPSGGAPLDAIAASLQRERRRTGLTLTELARRAGIAKSTLSQLE
                     SGTGNPSVETLWALSVALDVPFSRLVEPPRPRVQVIRRGEGPSVPSEHADYAATLLASS
                     PAHARRDIYLVTIQPGVPRRSDPHSPGVVEHVILSSGHARVGVAEEPVDLEPGDYTSYP
                     ADVPHVFEALAPDTVAVLVSEHD"
     gene            complement(916..1491)
                     /locus_tag="DMB38_RS08610"
                     /old_locus_tag="DMB38_08615"
     CDS             complement(916..1491)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971178.1"
                     /locus_tag="DMB38_RS08610"
                     /note="This protein contains a domain related to
                     Tuberculosis Necrotizing Toxin, which is the C-terminal
                     effector domain of outer membrane channel protein CpnT, and
                     which has a lethal NAD+-glycohydrolase activity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="DMB38_08615"
                     /product="TNT domain-containing protein"
                     /protein_id="WP_240662057.1"
                     /transl_table=11
                     /translation="MGASLRAAPVCTGERFGDARLGPEHLPGRWEPRVGPLLQGWHPTG
                     SLTPAAFLEKYWTGPAEGGTWKYPPNDGFAEVNGTVDREATRLEAGQLLDRFGSEYGGY
                     LAPAGDRYAERALPPQNLNTRDPDAPCDYRVYQVTKPFWVWQGAVAPWFEQPGGGEQIK
                     LDPVFLDPGEGQRLNVTWLLEHGYLAPA"
     gene            complement(1842..2504)
                     /locus_tag="DMB38_RS08615"
                     /old_locus_tag="DMB38_08620"
     CDS             complement(1842..2504)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659435.1"
                     /locus_tag="DMB38_RS08615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_08620"
                     /product="arsenate reductase ArsC"
                     /protein_id="WP_240662059.1"
                     /transl_table=11
                     /translation="MSDPGGQARPDPRAGHRLALHTAAKRLRTEFQGVFGEQTIERFLY
                     GAYGDIAVRGAPPDDLPRLTERYARRRLHALARVEGVSGDGRPVVLFLCVHNAGRSLMA
                     MGLTARLAGNRAVAWSAGSAPGTSANAAAVAVMGERGIDISGEFPTAWTDEVVRAADVI
                     VTMGCGDACPVFPGKRYVDWPLADPRGWAVAEVRPLRDEIERRVRKLLADLGIPPRG"
     misc_feature    complement(2424..2426)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            2670..3101
                     /locus_tag="DMB38_RS08620"
                     /old_locus_tag="DMB38_08625"
     CDS             2670..3101
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834467.1"
                     /locus_tag="DMB38_RS08620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_08625"
                     /product="SseB family protein"
                     /protein_id="WP_125934669.1"
                     /transl_table=11
                     /translation="MTEAPQGSDPSTTQRALEALATDAKDPAALHALAHSEVLVPVPDE
                     GPGQQEESAEQERAGAEESMMLPVMEQPNGDRLVPVFTSESRMGYALPSVDKYRKLPLV
                     LLANAWPSDEVYLTIDTGSPGALTLSAAGVRTLLGRSGG"
     gene            complement(3114..3488)
                     /locus_tag="DMB38_RS08625"
                     /old_locus_tag="DMB38_08630"
     CDS             complement(3114..3488)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834466.1"
                     /locus_tag="DMB38_RS08625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_08630"
                     /product="DUF6221 family protein"
                     /protein_id="WP_240662061.1"
                     /transl_table=11
                     /translation="MNNGFHEPAHRARDLVAFLRERYAEQRRTAVEQRLQHWQREVAEL
                     NAKQAVVDHCERWTLMADEAGRRIAERSAAGLGVEPAEALEAARLDGCATAVIHVVATL
                     ALPFQRHPGYREAWRPPPVP"
     gene            complement(3645..4091)
                     /locus_tag="DMB38_RS08630"
                     /old_locus_tag="DMB38_08635"
     CDS             complement(3645..4091)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834465.1"
                     /locus_tag="DMB38_RS08630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_08635"
                     /product="hypothetical protein"
                     /protein_id="WP_125934671.1"
                     /transl_table=11
                     /translation="MTSPTASSHGTTSSSPTPPHRGGSPWATGGTVFAGVMMLVYGVLA
                     VFQGIAGIAHDDVYARQGDYVFKFDLTTWGWIHLILGVVVAAAGLGILMGNEWARLLGV
                     ALAALAVIANFAWLPYLPVWAIVNIAIGVFVIWALCTDRSRSLV"
     gene            complement(4240..5004)
                     /locus_tag="DMB38_RS08635"
                     /old_locus_tag="DMB38_08640"
     CDS             complement(4240..5004)
                     /GO_function="GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /GO_process="GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018841684.1"
                     /locus_tag="DMB38_RS08635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_08640"
                     /product="potassium channel family protein"
                     /protein_id="WP_125934672.1"
                     /transl_table=11
                     /translation="MKDGRDEADDVRGDGRDGARGDGRDGARGEGRDGAGGGDRDPRAQ
                     GRRRDPLLPWLREAVPVAIMVVAYFLLPLHLLGAERPELSWTLFVLALGAVTLLLLREI
                     RAVLMDVPHARPGVTIPLLMIASLLVFSAAYSSIARQSGEFHGLRTRVDALYFTVATVA
                     TVGYGDITAQGQTARILVMGQITYGFIFLTAAATALSRRLRRALGERQTPDGRRHGRGR
                     RGRRHGDRRRGGGTGPGGGRPDGGAGRGTGRA"
     gene            complement(5001..5798)
                     /locus_tag="DMB38_RS08640"
                     /old_locus_tag="DMB38_08645"
     CDS             complement(5001..5798)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834463.1"
                     /locus_tag="DMB38_RS08640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_08645"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_125934673.1"
                     /sec_met_domain="Linocin_M18 (E-value: 2.9e-88, bitscore:
                     294.0, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRELAPITQAAWAEIEEEARRTFRRHVAGRRVVDVTGPEGP
                     ELAAVGTGHLEEIAPPAPGATARLRAAQSLIELRVPFTVSRDAVDDVERGSRDSDWQPV
                     KDAARTAAFAEDRMIADGYAAAGVEGLRTRSSLPELSLPAEVRDYPDTVSRALSALRLA
                     GVDGPYSLLLGADAYTAVSETADHGYPVAQHISRLLDGELIWAPAMTGGLALSTRSGDF
                     ELRLGEDLAIGYTAHDAKTVQLYLHETLTFLTYTDEAIVVLRA"
     gene            complement(5795..6847)
                     /locus_tag="DMB38_RS08645"
                     /old_locus_tag="DMB38_08650"
     CDS             complement(5795..6847)
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834462.1"
                     /locus_tag="DMB38_RS08645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_08650"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_125934674.1"
                     /transl_table=11
                     /translation="MPEPQPVLAPPATAAVFLVVTVAPGGEDTIRDVLEDLPALQRAVG
                     FGAPDDDLTCVAGIGAAVWGRLFQGPRPAGLHPFRELAGERHRAVATPGDVLFHIRSRR
                     MDLCFELARRLRARLGDAVAVADEVHGFKYYDMRDLLGFVDGTENPVGRAAGAAVFTGP
                     EDPRFAGGSYVMVQKYVHDLDAWEALSTEEQERVIGRTKLGNTELPEGVQPEDSHVALN
                     TITGPGGEERQVVRDNMPFGSVGEGEFGTYFIGYAREPGVLEEMLENMFLGRPPGTYDR
                     ILDFSTAVTGCLFFAPAAGFLDDLPALPAALGAAAAAVADPVAPAPVPDPQGAGDGSLG
                     IGGLKGAPAR"
     gene            complement(6968..7342)
                     /locus_tag="DMB38_RS08650"
                     /old_locus_tag="DMB38_08655"
     CDS             complement(6968..7342)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834460.1"
                     /locus_tag="DMB38_RS08650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_08655"
                     /product="DUF6401 family natural product biosynthesis
                     protein"
                     /protein_id="WP_125934675.1"
                     /transl_table=11
                     /translation="MNVGGPGDEAVGCPLTAVAARYLPRLSEFAFEPGLVAAVDQHAAA
                     VRDAIVPRQRRPWHDLLPQRIERVCAPQRMRREDLTDYVVGFTDVLAESDWREPVGYDF
                     AVLRLTAVCWLIREYDLLSG"
     misc_feature    complement(7462..7476)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: strong, score: 30.11"
                     /tool="antismash"
     misc_feature    7463..7477
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: strong, score: 29.02"
                     /tool="antismash"
     gene            7539..8558
                     /locus_tag="DMB38_RS08655"
                     /old_locus_tag="DMB38_08660"
     CDS             7539..8558
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018853989.1"
                     /locus_tag="DMB38_RS08655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_08660"
                     /product="glycoside hydrolase family 6 protein"
                     /protein_id="WP_125934676.1"
                     /transl_table=11
                     /translation="MRVRILRRWRRTALTGLLGAGLALAGLATAAPAAPVAPAARAAEP
                     DSGAAAAPHASAALPGPLYVDPDSQVRRWTAANPGDWRQPVIADRIASQPQARWLSQYN
                     PGTVQQEVAGYMSAAGDALPVLSVYGIPNRDCGGASSGGAPDLPSYQNWIRLIAAGLGD
                     HPVVIILETDALALEDCLDQAGRDARHAALGEAVTTLKAAGPQTHVYLDGGHSAWHSPG
                     DQAARLAASGVAEADGVFTNVSNFRTTAQEVAYGKQILAALGDPDLSLVVDTSRNGNGS
                     NGQWCDPSGRALGAEPTLTTGDDAVDALLWVKPPGEADGCAARAGQFVPDLAYALATN"
     gene            complement(8647..10461)
                     /locus_tag="DMB38_RS08660"
                     /old_locus_tag="DMB38_08665"
     CDS             complement(8647..10461)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="DMB38_RS08660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="DMB38_08665"
                     /product="2TM domain-containing protein"
                     /protein_id="WP_125934677.1"
                     /transl_table=11
                     /translation="MEAFPPMPSEPPAEPERGAGSGQQPTEPDILAFGPDRPVFELAPV
                     PVRARDPYAVGLANASLLGIGYGMLRRPRIAFGAAGVSVMLVILLATAGRNAWFQAVVL
                     AWWIGVTVHGWYLAGGRLPRRGAAAPEASAQATEVQPAEPAQPVAAAHAAEREPADAPP
                     PPSAPPVLPANVRRDRLIALLVAAPVLLACTALRLDASRIEGNAADAHRAGECAQAVSD
                     LEGLWFGHRLADAPLTARAEDGTEACELLLKAERQAEDDRIRGARTLKAYADHPGALWD
                     GAAGRRADLLLAQAEDELDAGLTGDTDALSAGFGHLATVLDEYAGGDAEGGEDRSGDVD
                     KALDGFLGALPTDDACQTKEVTDWLGAHETGDDGLARAADVVPEVAPAAIVDCGDALMA
                     DDTWKQARTQYEQLLDEYPDSELAGAAKDGVERADLAIQLDKVRGLLKPGEYGEKPAYC
                     DNPAPYRGAKPYRGEGPHRAMLIGQGKHRSKLPSSWLAKDPKDAVAIICAGSSKHGATV
                     ANCPYEDDLAIGGSVNVAFKNREIPVRVYELRTGKLAKKTTVEIGGASCPAVLEYTYYG
                     VDTGPPSEVYVKSSESDVRAGYEPLIYP"
     misc_feature    10448..10459
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.11"
                     /tool="antismash"
ORIGIN
        1 ggaggcgacg atgccgacga acatgaactg cgccgccccc gcgaagacca ccaccgacag
       61 cagcaccggc agccacagcg gcagcccctc gccgaccgcg atggccccga acgagacgcc
      121 gaccagggtg tcggcggcgc agacgagcgc gatgtcgcgg gcgaggtcgc cgcgcagggt
      181 tcgccatatc gaacgcatga ctgccatgct gaacatccgc tactctgttc gtcaagtcga
      241 ataaacgacc gacgaagcga acggagcgtg ccatggtccc gagcggagga gcccccctcg
      301 acgccatcgc ggcgtcgctc cagcgcgagc gccgccgtac ggggctgacg ctgacggagc
      361 tggcccgccg cgcgggcatc gccaagtcga cgctctccca gctcgaatcc ggcaccggca
      421 acccgagcgt ggagaccctg tgggccctca gcgtcgcgct cgacgtgccc ttctcccggc
      481 tggtcgagcc gccccgcccg cgagtgcagg tcatacgccg cggcgagggc ccgtccgtgc
      541 cctccgagca cgccgactac gcggcgacgc tgctcgcctc ctcccccgcc cacgcccgcc
      601 gggacatcta cctcgtcacc atccagcccg gcgtcccgcg ccgctcggat ccgcactcac
      661 ccggcgtcgt ggagcacgtg atactcagca gcgggcacgc gcgcgtgggc gtggcggaag
      721 aaccggtcga cctggagccc ggcgactaca cctcgtaccc tgccgacgtc ccgcacgtct
      781 tcgaggccct ggcgccggac acggtcgccg tcctcgtctc cgagcacgac tgacgccgcg
      841 ccgtccccgc gggccggccg gtcagccggc ctgcgggcct gccgggaccg ccgggcgtcg
      901 cacaggcaca aggcgttacg cgggcgccag gtagccgtgc tccagcagcc aggtgacgtt
      961 cagccgctgc ccctcgcccg ggtcgaggaa gaccgggtcg agcttgatct gctcgccccc
     1021 gccgggctgc tcgaaccacg gcgcgaccgc cccctgccag acccagaacg gcttcgtgac
     1081 ctggtagacg cggtagtcgc agggggcgtc gggatcgcgg gtgttgaggt tctgcggagg
     1141 cagcgcgcgc tcggcgtagc ggtcgccggc gggagcgagg tagccgccgt actccgagcc
     1201 gaagcggtcg aggagctgcc cggcctccag ccgcgtcgcc tcccggtcga cggtgccgtt
     1261 gacctcggcg aagccgtcgt tcggcgggta cttccaggtg ccgccctcgg cggggcccgt
     1321 ccagtacttc tccaggaacg cggccggcgt gagcgaaccg gtgggatgcc agccctgcag
     1381 cagcggcccc acccgcggct cccaccggcc cggcaggtgc tccgggccca gccgggcgtc
     1441 gccgaaccgc tcgccggtgc agacgggcgc ggcccggagg gaggccccca cggcgggcgc
     1501 gggcacgggg gccgcgcggc cggtggccgg gggtggacgg ccgaggggcg ggtcgcgggg
     1561 tcggggtgcc cggcggcgcc cgccgcggcg gggacgccgc cggccagccc cgcggcggcg
     1621 acgaggacgg caagtgccgt gcgtacggtg cgggttcgcg tcacggtggg ggttcgcttc
     1681 acggtggggc ccttcgcgct cggcggtcgg cggccccacc atgatccgca ggtcgggcgg
     1741 cgggcccgca gaccaacacg cgcgccaccg cccgcccttc gggtgaaccg ggcgcgcccg
     1801 ggcgggcgta cggaaccggc gggcgcggcc gtccgccgcc gtcagccgcg gggcggtatg
     1861 cccaggtccg cgaggagttt gcggacccgc cgctcgatct cgtcccgcag cggccgcacc
     1921 tccgccaccg cccacccgcg cggatcggcc agcggccagt ccacgtaccg cttgccgggg
     1981 aacaccgggc aggcgtcccc gcagcccatc gtgacgatca cgtccgccgc ccggacgacc
     2041 tcgtccgtcc acgccgtggg gaactcgccg gagatgtcga tcccccgctc ccccatcacg
     2101 gccacggccg ccgcgttggc cgaggtgccg ggcgcggagc ccgccgacca ggccaccgcg
     2161 cggttccccg ccagccgggc cgtcaggccc atcgccatca ggctgcgccc ggcgttgtgc
     2221 acgcagagga agagcacgac cggccgcccg tcgccgctca ccccctccac ccgggccagc
     2281 gcgtgcagcc ggcggcgcgc gtagcgctcg gtgaggcgcg gcaggtcgtc cggcggggcg
     2341 ccgcgtaccg ctatgtcccc gtacgcgccg tacaggaacc gctcgatggt ctgctcgccg
     2401 aacaccccct ggaactccgt ccgtaacctc ttggcggcgg tgtgcagcgc gagccggtga
     2461 ccggctctgg gatcgggtcg tgcctggccc ccggggtcac tcatcgcccg gattatggtc
     2521 cctccgggcc gcgcgcacac ccggaacggc tccgtcaccc gctccgggag tgacagcggc
     2581 ggcacgttcg acgctgtacg gggtaggcaa tgccgcgggc gggccggaag ggactgcgcg
     2641 ccgcggcaca ccggaggaag cgaaggacca tgaccgaggc accgcagggg agcgacccct
     2701 cgaccactca gcgggcgctg gaggcactgg cgaccgacgc caaggacccg gcggcgctgc
     2761 acgcgctggc ccacagcgaa gtcctcgtgc ccgtgccgga cgaaggaccg ggtcagcagg
     2821 aggagagcgc ggagcaggag cgggccggcg ccgaggagag catgatgctg ccggtgatgg
     2881 agcagccgaa cggcgaccgg ctcgtgcccg tcttcacgtc cgagtcccgg atggggtacg
     2941 cgctgccctc cgtcgacaag taccgcaagc tgccgctggt gctgctggcc aacgcctggc
     3001 cgtcggacga ggtgtatctg accatcgaca ccggcagtcc cggcgcgctg accctgagcg
     3061 ccgcgggcgt acgcaccctc ctcggccgct ccggcggctg accccgccac gccctacggc
     3121 accggcggcg ggcgccacgc ctcccggtac ccggggtggc gctggaacgg cagcgccagc
     3181 gtcgccacga cgtggatcac cgccgtcgcg cagccgtcca gccgggccgc ctccagcgcc
     3241 tcggccggct cgacgccgag cccggcggcg gaccgctccg cgatcctgcg gcccgcctcg
     3301 tcggccatga gggtccaccg ctcgcagtgg tccacgacgg cctgcttggc gttcagctcg
     3361 gcgacctcgc gctgccagtg ctgcaaccgc tgctcgacgg cggtcctgcg ctgctccgcg
     3421 taccgctcgc ggaggaacgc gaccagatcg cgggcgcggt gggccggctc gtggaaaccg
     3481 ttgttcatgt ctgcggcgct gacggtgcac acctccacac gacggggctc ccgttgttca
     3541 gcgtcgccgc gtggaaaggt gtcacaccgt accggccgga gcgggcgctt ccgcaggggg
     3601 gagcggaggc gcccgccgcc ggggccgccg cggtccgcgc agcctcagac gagcgagcgg
     3661 gaccggtcgg tgcacagcgc ccagatcacg aagacgccga tggcgatgtt cacgatcgcc
     3721 cacaccggca ggtacggcag ccacgcgaag ttggcgatca ccgccagggc ggccagcgcg
     3781 acgcccagca gccgggccca ctcgttgccc atgaggatgc ccaggcccgc cgcggcgacg
     3841 acgacgccga gtatcaggtg gatccagccc caggtcgtca ggtcgaactt gaacacgtag
     3901 tcgccctgcc gggcgtacac gtcgtcgtgg gcgatgcccg cgatgccctg gaacacggcg
     3961 aggacgccgt acaccagcat catcaccccg gcgaacaccg tgccccctgt cgcccagggg
     4021 ctgccgccgc ggtgcggtgg ggtggggctc gacgacgtcg tgccgtggga ggaggcggtg
     4081 ggggaggtca tcggaacaca ctccttaccg agggccgccg gggggtggcc aggagtccca
     4141 gcatcacgcc gcggcccggg gcggacgatc ccgtgacggc cgtacgggtg aacgccccgt
     4201 ggccccggcg ccgcacgcgc ccgggccccg cgcctcgcct caggcccgcc ccgtgccccg
     4261 gccggcgccc ccgtccggcc ggccgccgcc cggccccgta ccgccgccgc gccgccggtc
     4321 gccgtgccgc cgcccgcgcc gcccccggcc gtgccgccgc ccgtccggcg tctgccgctc
     4381 cccgagcgcc cggcgcagcc ggcggctcag cgcggtcgcg gcggcggtga ggaagatgaa
     4441 gccgtacgtg atctgcccca tcaccaggat cctggccgtc tgcccctgcg cggtgatgtc
     4501 gccgtagccg acggtcgcca cggtcgcgac cgtgaagtag agcgcgtcga cccgggtccg
     4561 cagcccgtgg aactcccccg actgccgggc gatcgagctg tacgcggcgg agaagacgag
     4621 gagcgaggcg atcatcagca gcgggatggt gaccccgggc ctggcgtgcg gcacgtccat
     4681 cagcaccgcc cggatctcgc ggagcagcag cagggtcacc gcgcccagcg ccagcacgaa
     4741 gagcgtccag ctcagctcgg gccgctccgc gcccaggaga tgcaggggca gcaggaagta
     4801 cgcgacgacc atgatcgcca cgggtacggc ctcgcgcagc caggggagca gcggatcccg
     4861 gcggcggccc tgcgcgcggg ggtcccggtc accgcccccg gcgccgtcgc ggccctcgcc
     4921 ccgggcgccg tcgcggccgt cgccccgggc gccgtcgcgg ccgtcgcccc ggacgtcgtc
     4981 ggcctcgtcg cgcccgtcct tcacgcccgt aggacgacga tcgcctcgtc cgtgtacgtc
     5041 aggaacgtca gcgtctcgtg caggtagagc tgcaccgtct tcgcgtcgtg cgcggtgtac
     5101 ccgatggcca ggtcctcgcc gaggcgcagc tcgaagtcgc cgctgcgggt ggagagcgcg
     5161 agcccgccgg tcatcgccgg agcccagatc agctcgccgt ccagcagccg ggagatgtgc
     5221 tgcgcgaccg gatagccgtg gtcggcggtc tcgctcaccg ccgtgtacgc gtccgccccc
     5281 agcagcagcg agtacgggcc gtccaccccg gccagccgca gcgcggagag tgcccggctg
     5341 acggtgtcgg ggtagtcgcg gacctcggcg ggcaggctca gctcgggcag cgagctgcgc
     5401 gtgcgcagtc cctcgacgcc tgcggccgcg tacccgtcgg cgatcatccg gtcctcggcg
     5461 aacgctgcgg tgcgggcggc gtccttgacc ggctgccagt cggagtcccg ggagccgcgc
     5521 tccacgtcgt ccacggcgtc gcggctgacg gtgaacggca cccgcagctc gatcagcgac
     5581 tgcgccgcgc gcagccgcgc cgtcgcaccg ggcgcgggcg gggcgatctc ctccaggtgc
     5641 ccggtgccga cggcggccag ctccgggccc tcgggcccgg tgacgtcgac gacgcgacgg
     5701 cccgcgacat ggcggcggaa ggtgcgccgg gcctcctcct cgatctccgc ccaggccgcc
     5761 tgggtgatcg gagcgagttc gcggtgcagg ttgttcatcg tgcgggtgct cctttgaggc
     5821 cgccgatccc cagcgagccg tcaccggcgc cctgcgggtc gggtacgggg gccggggcga
     5881 cggggtccgc aacggcggcc gccgccgcgc cgagggcggc cggcagggcg gggaggtcgt
     5941 cgaggaagcc ggccgcgggg gcgaagaaca ggcagccggt cacggccgtg gagaagtcca
     6001 ggatccggtc gtacgtcccg ggcgggcggc cgaggaacat gttctccagc atctcctcca
     6061 gcaccccggg ctcgcgcgcg tagccgatga agtacgtgcc gaactcgccc tcgccgacgg
     6121 agccgaacgg catgttgtcc cgtacgacct ggcgctcctc gccgccgggg ccggtgatcg
     6181 tgttgagcgc gacgtgcgag tcctccggct gcacgccctc cggcagctcg gtgttgccca
     6241 gcttggtgcg cccgatgacc cgctcctgct cctcggtgct cagcgcctcc caggcgtcga
     6301 ggtcgtgcac gtacttctgc accatcacgt agctgccgcc ggcgaaccgc gggtcttcgg
     6361 gtcccgtgaa gaccgcggcg ccggcggccc gtccgaccgg gttctcggtg ccgtcgacga
     6421 agccgagcag gtcgcgcatg tcgtagtact tgaagccgtg cacctcgtcg gcgaccgcga
     6481 cggcgtcgcc gagccgggcg cgcagccgtc tggccagctc gaagcagagg tccatgcgcc
     6541 gggagcggat gtggaagagc acgtcgccgg gggtggcgac ggcgcggtgg cgctcgcccg
     6601 ccagctcgcg gaacgggtgc agccctgccg ggcgcgggcc ctggaagagc cggccccaga
     6661 cggcggcgcc gatgcccgcg acgcacgtca gatcgtcgtc gggggcgccg aagcccacgg
     6721 cgcgctggag cgcggggagg tcttcgagga cgtcgcggat cgtgtcctcg ccgccgggcg
     6781 cgacggtgac gacgaggaac accgcggcgg tggcgggcgg ggccagcacg ggctggggtt
     6841 cgggcacggt ccctcctggt cacgggggca ttcgtgacga tacgggacac acaggacagc
     6901 gcaatccggc ggcgggccgc gttcacccgc ccgtgcggcg gggagtcgta cgggggactt
     6961 cgccgggtca gcccgacagg aggtcgtact cgcggatcag ccagcagacc gccgtcagcc
     7021 gcagcaccgc gaagtcgtag ccgaccggct cgcgccagtc gctctcggcc agcacgtcgg
     7081 tgaacccgac cacatagtcc gtcagatcct cgcggcgcat ccgctgcggc gcgcagacgc
     7141 gctcgatccg ctgcggcagg aggtcgtgcc agggacggcg ctgccggggc acgatcgcgt
     7201 cgcgtacggc ggccgcgtgc tggtcgacgg ccgcgacgag ccccggctcg aaagcgaact
     7261 ccgacagccg gggcaggtac ctggcggcga cggcggtcag cgggcagccg acggcttcat
     7321 ccccgggtcc tcccacgttc accggtcgac tgtaacccgg atcccgcggg cctcccgtcg
     7381 tgaggtacgt cccacgcgcc gtcgtcccgc gcgcgggcgg tgtttccaac ccggcggtcc
     7441 gccactatgc tccggcagcg catgggagcg ctcccagaac cgcacacctc ccacccggag
     7501 acccccgtct ccggcagacg cgacaaggac ggaccaccat gcgcgtacgc atcctgcgcc
     7561 gatggcgccg caccgcactc accggcctcc tcggcgccgg cctggccctc gccggcctcg
     7621 ccaccgcggc accggcggcc ccggtggccc cggcggcacg tgcggccgag ccggactccg
     7681 gggccgccgc cgccccgcac gcctccgccg cactgcccgg ccccctgtac gtcgaccccg
     7741 actcccaggt ccggcggtgg accgcggcca accccggcga ctggcggcag ccggtcatcg
     7801 ccgaccgcat cgcctcccag ccgcaggccc gctggctctc ccagtacaac cccggcaccg
     7861 tgcagcagga ggtcgccggc tacatgtccg ccgccggcga cgccctgccc gtgctctccg
     7921 tctacggcat ccccaaccgc gactgcggcg gcgccagctc cggcggcgcc cccgacctcc
     7981 cctcgtacca gaactggatc cggctcatcg ccgccggcct cggcgaccac cccgtcgtga
     8041 tcatcctgga gaccgacgcg ctcgccctgg aggactgcct cgaccaggcg ggccgcgacg
     8101 cccggcacgc ggcgctgggg gaggccgtca ccaccctgaa ggccgcgggc ccgcagaccc
     8161 acgtctacct cgacggcggc cactccgcct ggcactcccc cggcgaccag gccgcccggc
     8221 tggcggcgtc cggggtcgcg gaggcggacg gcgtgttcac caacgtctcc aacttccgca
     8281 ccacggcgca ggaggtggcg tacggcaagc agatcctggc cgcgctcggc gacccggacc
     8341 tcagcctggt cgtggacacc agccgcaacg gcaacggctc caacggccag tggtgcgacc
     8401 cctcgggccg cgcgctgggc gcggaaccga cgctcacgac cggcgacgac gccgtggacg
     8461 cgctgctgtg ggtcaagccg cccggcgagg cggacggctg cgcggcgcgg gcggggcagt
     8521 tcgtccccga cctggcgtac gcgctggcga ccaactgagc ccgcctgcca ccggccggcg
     8581 ttcagccggg ccggtccggg gcggccgcca ccgacccgga ccggacccgg ccctcagggg
     8641 tggccctcag ggatagatca gcggctcgta gcccgcgcgc acgtcggact ccgaggactt
     8701 gacgtacacc tcggacggcg ggccggtgtc gacgccgtag tacgtgtact ccagcacggc
     8761 cgggcaactg gcgccgccga tctcgacggt ggtcttcttc gccagcttgc cggtgcgcag
     8821 ctcgtagacc cggacgggga tctcccggtt cttgaaggcc acgttgacgg agccgccgat
     8881 ggccaggtcg tcctcgtacg ggcagttcgc caccgtggcg ccgtgcttcg aggagcccgc
     8941 gcagatgatc gccaccgcgt ccttggggtc cttcgcgagc caggaggacg gcagcttcga
     9001 gcggtgcttg ccctggccga tgagcatcgc gcggtgcggt ccttcgccgc ggtagggctt
     9061 ggcgccgcgg tacggggccg ggttgtcgca gtacgcgggc ttctctccgt actcgccggg
     9121 cttcagcagg ccgcgcacct tgtcgagctg gatggccagg tccgcgcgct cgaccccgtc
     9181 cttcgccgca ccggccagct cgctgtcggg gtactcgtcg aggagctgct cgtactgcgt
     9241 gcgggcctgc ttccaggtgt cgtcggccat cagcgcgtcg ccgcagtcga cgatcgcggc
     9301 gggcgcgacc tcgggcacga cgtcggcggc gcgcgccagg ccgtcgtccc cggtctcatg
     9361 ggcgccgagc cagtcggtga cctccttggt ctggcaggcg tcgtcggtgg ggagcgcgcc
     9421 gaggaacccg tcgagcgcct tgtcgacgtc gccgctgcgg tcctcgccgc cttcggcgtc
     9481 gccgcccgcg tactcgtcga ggacggtggc gagatggccg aaacccgcgc tcagcgcgtc
     9541 ggtgtcgccg gtcagtccgg cgtccagctc gtcctcggcc tgggcgagga ggaggtcggc
     9601 ccggcggccg gcggcgccgt cccagagcgc gccggggtgg tcggcgtacg ccttgagcgt
     9661 gcgggcgccg cgtatgcggt cgtcctccgc ctggcgctcg gccttgagca gcagctcgca
     9721 cgcctcggtg ccatcctcgg cgcgggcggt cagcggggcg tcggcgaggc ggtggccgaa
     9781 ccagagtcct tcgaggtcgg agacggcctg ggcgcattcg cccgcgcggt gggcgtcggc
     9841 ggcgttgccc tcgatccggg aggcgtcgag gcgcagggcg gtgcaggcga ggaggacggg
     9901 agcggcgacg aggagggcga tgaggcggtc gcggcgcacg ttcgcgggga ggacgggggg
     9961 tgcggagggg ggcgggggcg cgtcggcggg ctcgcgctcc gcggcgtgcg cggctgcgac
    10021 gggctgcgcg ggctccgccg gctgtacctc cgtggcctgc gcggacgcct ccggcgcggc
    10081 ggcgccgcgg cggggcaggc ggcccccggc gagataccag ccgtgcacgg tgacgcctat
    10141 ccaccacgcc agcacgacgg cctggaacca tgcgttccgt ccggccgtcg ccagcaggat
    10201 gacgagcatg accgacacgc ccgcggcgcc gaaggcgatc cgcggcctgc ggagcatccc
    10261 gtaaccgatg cccagcagcg aggcgttggc gagcccgacg gcgtacgggt ccctggcccg
    10321 tacgggcacg ggcgccagct cgaacaccgg gcggtcgggg ccgaacgcga ggatgtcggg
    10381 ttcggtgggc tgctgcccgg agccggcgcc ccgttcgggt tcggcgggcg gctcgctggg
    10441 catcggggga aacgcttcca tcgtgatccc tttctcgggc gagccaccgg cgggccgtcc
    10501 ccatctttcg gcaccggagc cgggaggggt cccggaacac ccgccccccg gcgggcacat
    10561 gagccgccag ggagatcgtg gcccatcgcg gccccgccga acaggcagaa cccggccttg
    10621 ccgcgggtca cggaagcgca cccggcgccg cgcgcccctc acggggcggg tgcgccgagt
    10681 gggacctgtg aggacttgcg gagtgcgctt gcggcggcgt aggagcgacg gcggatgtgc
    10741 cgcagcgcgc gctgcggcac atccgccacc cggctgccgt ccggtcgcgg gtatgccc
//
