LOCUS       NZ_CP029618            10399 bp    DNA     linear   CON 19-MAY-2024
DEFINITION  Streptomyces sp. WAC 06738 chromosome, complete genome.
ACCESSION   NZ_CP029618
VERSION     NZ_CP029618.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. WAC 06738
  ORGANISM  Streptomyces sp. WAC 06738
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8324535)
  AUTHORS   Waglechner,N. and Wright,G.D.
  TITLE     Evolution of GPA BGCs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8324535)
  AUTHORS   Waglechner,N. and Wright,G.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2018) Michael G DeGroote Institute for Infectious
            Disease Research, McMaster University, 1280 Main St West, Hamilton,
            Ontario L8S 4L8, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. Jun-2017
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 148.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003945505.1-RS_2024_05_19
            Annotation Date                   :: 05/19/2024 05:01:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,191
            CDSs (total)                      :: 7,115
            Genes (coding)                    :: 6,917
            CDSs (with protein)               :: 6,917
            Genes (RNA)                       :: 76
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 198
            CDSs (without protein)            :: 198
            Pseudo Genes (ambiguous residues) :: 0 of 198
            Pseudo Genes (frameshifted)       :: 107 of 198
            Pseudo Genes (incomplete)         :: 115 of 198
            Pseudo Genes (internal stop)      :: 5 of 198
            Pseudo Genes (multiple problems)  :: 27 of 198
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:28:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1324071
            Orig. end                         :: 1334470
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP029618.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Gerard D Wright, McMaster
            University, 1280 Main St West, Hamilton ON, Canada.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="3"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5399
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(361..1335)
                     /locus_tag="DMB38_RS05500"
                     /old_locus_tag="DMB38_05515"
     CDS             complement(361..1335)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835759.1"
                     /locus_tag="DMB38_RS05500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_05515"
                     /product="SCO1860 family LAETG-anchored protein"
                     /protein_id="WP_125934190.1"
                     /transl_table=11
                     /translation="MPATAVTAAALAAGPALLLAAPVHADGADGAAPARTGSAGASVLR
                     ADLDVSLLDKTVRVPVLSTLNEVRVPAAGRDSGAADKTALDVRVDGLAAAEGGEHPEGP
                     RAVNIARAEVATADATVDVYGAEASSHLARAEVHVPGLPALPLIKVEEVTSKAVCAVGE
                     KPKAESRTLGSVTVLGKRVTVTVGGEPTVVAVPGVGEVSLGFSQTETTRAAASAAALRL
                     RVSVNPLKLNVAEVEGEVTLVEAACETPAGGTGPGEEPGDRPQGDAEPVQDERPADATD
                     PAPERQLAETGGSSATPYLVGGAALLVGLGLSGLVLARIRTRG"
     misc_feature    complement(1336..1351)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.2"
                     /tool="antismash"
     misc_feature    complement(1369..1384)
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 22.24"
                     /tool="antismash"
     misc_feature    complement(1422..1435)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: medium, score: 20.37"
                     /tool="antismash"
     gene            complement(1517..2608)
                     /locus_tag="DMB38_RS05505"
                     /old_locus_tag="DMB38_05520"
     CDS             complement(1517..2608)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835758.1"
                     /locus_tag="DMB38_RS05505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_05520"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_125934191.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 3.7e-15, bitscore:
                     54.7, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSESPVLRVRGRVLTGREEVRDELWVVGGRVTFTPPAAAREVVDI
                     EGWALPGLVDAHCHVGLDAHGAVDDATSEKQARTEREAGALLLRDAGSAADTRWIDDRD
                     DLPRLVRAGRHIARTRRYIRNYAHEIEPDDLVAYVAREARRGDGWVKLVGDWIDREAGD
                     LTPCWPREALAPAIAEAHRFGARVTAHCFAEDSLRDLVEAGIDCIEHATGLTEETVPLF
                     AERGVAIVPTLVNIATFPELAAGGQAKFPRWADHMRRLHDRRYATVRAAYDAGIPVYAG
                     TDAGGSLAHGLVGQEVGELVAAGIPAADAVSAAAWGAREWLGRPGLEEGAPADLVVYEA
                     DPLADVRVLTAPRRIVLRGRVVG"
     misc_feature    2701..2716
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.04"
                     /tool="antismash"
     misc_feature    2708..2725
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.33"
                     /tool="antismash"
     misc_feature    complement(2712..2729)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.48"
                     /tool="antismash"
     misc_feature    2787..2803
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.19"
                     /tool="antismash"
     misc_feature    2792..2806
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.65"
                     /tool="antismash"
     misc_feature    2811..2825
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.21"
                     /tool="antismash"
     misc_feature    2836..2853
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.71"
                     /tool="antismash"
     misc_feature    complement(2885..2899)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.74"
                     /tool="antismash"
     gene            3019..3573
                     /gene="ectA"
                     /locus_tag="DMB38_RS05510"
                     /old_locus_tag="DMB38_05525"
     CDS             3019..3573
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059805.1"
                     /locus_tag="DMB38_RS05510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_05525"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_125934192.1"
                     /transl_table=11
                     /translation="MTAAQADLAGPPDKPRTAAADEAGVRLDTPRIEDGAAIWRIARDS
                     GSLDLNSSYSYLLWCRDFADTSVVARDSDGAPVGFVTGYIRPARPETLVVWQIAVDAAF
                     RGRGLAGALLDGLTERALADRGIRRLETTITPDNSASDQLFRSYARRHGAGVDRDVLFQ
                     GEHFPDGHEPELLYRIGPLAG"
     gene            3629..4897
                     /gene="ectB"
                     /locus_tag="DMB38_RS05515"
                     /old_locus_tag="DMB38_05530"
     CDS             3629..4897
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 2.8e-71.
                     Score: 232.2. Matches aSDomain:
                     nrpspksdomains_DMB38_RS05515_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="DMB38_RS05515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_05530"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_125934193.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.1e-73, bitscore:
                     245.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRGWPAVFDRAQGSYLYDEDGHTY
                     LDFFAGAGSLNYGHNNAVLKRALLDYLERDGIVHGLDMATTAKRAFLETFQNVVLRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYLDGQVPDFLWFERLLEDQGSGLNKPAAVIVETIQGEGGINVARA
                     DWLRGLAELCKRRDMLLIVDDIQMGCGRTGGFFSFEDAGITPDIVTLSKSISGYGLPMS
                     LTLFKPELDIWEPGEHNGTFRGHNPAFVTATAALDTYWADGQMEKQTLARGAQIEQALV
                     AICAEHSDADATYRGRGMVWGLEFPDPARAARVCRRAFELGLILETSGPQSEVVKLLPA
                     LTTTPDDLDEGLRTLARAVRETA"
     aSDomain        3713..4723
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DMB38_RS05515_Aminotran_3.1"
                     /evalue="2.80E-71"
                     /label="DMB38_RS05515_Aminotran_3.1"
                     /locus_tag="DMB38_RS05515"
                     /protein_end="365"
                     /protein_start="28"
                     /score="232.2"
                     /tool="antismash"
                     /translation="FDRAQGSYLYDEDGHTYLDFFAGAGSLNYGHNNAVLKRALLDYLE
                     RDGIVHGLDMATTAKRAFLETFQNVVLRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYLDGQVPDFLWFER
                     LLEDQGSGLNKPAAVIVETIQGEGGINVARADWLRGLAELCKRRDMLLIVDDIQMGCGR
                     TGGFFSFEDAGITPDIVTLSKSISGYGLPMSLTLFKPELDIWEPGEHNGTFRGHNPAFV
                     TATAALDTYWADGQMEKQTLARGAQIEQALVAICAEHSDADATYRGRGMVWGLEFP"
     gene            5001..5399
                     /locus_tag="DMB38_RS05520"
                     /old_locus_tag="DMB38_05535"
     CDS             5001..5399
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007498584.1"
                     /locus_tag="DMB38_RS05520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_05535"
                     /product="ectoine synthase"
                     /protein_id="WP_125934194.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.8e-53, bitscore:
                     178.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKELEGTDRHIKSASGTWESKRIVLAKERVGFSLHETVLY
                     AGTETSMWYANHIEAVVCVEGEAELTNDETGEKHAITPGTMYLLDGHERHTMRIKEDFR
                     CICVFNPPVTGREDHDENGVYPLLTEED"
     gene            5405..6298
                     /gene="thpD"
                     /locus_tag="DMB38_RS05525"
                     /old_locus_tag="DMB38_05540"
     CDS             5405..6298
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835754.1"
                     /locus_tag="DMB38_RS05525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_05540"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_125934195.1"
                     /sec_met_domain="PhyH (E-value: 3.6e-39, bitscore: 133.9,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASPTVTDLYPTRGAEEVSTPRQDPVVWSEPGADGPIPPWELEGY
                     ERNGYLTVDQLITPEEVETYKAELDRLIADPRVRADERSIIEAGTQAVRSIFEIHKISD
                     VFGKLVRDERVLERARQILGSDVYIHQSRINIKPGFGASGFYWHSDFETWHAEDGLPNM
                     RTVSVSIALTENYETNGGLMIMPGSHKTFLGCAGATPKDNYKKSLQMQDAGTPSDEALT
                     EMAEAHGIKLFTGAAGGATWFDCNCMHGSGDNITPYPRSNVFLVFNSVENAAVEPFAAP
                     VRRPDFIGARDFTPVR"
     gene            complement(6441..7148)
                     /locus_tag="DMB38_RS05530"
                     /old_locus_tag="DMB38_05545"
     CDS             complement(6441..7148)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835753.1"
                     /locus_tag="DMB38_RS05530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_05545"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_125934196.1"
                     /transl_table=11
                     /translation="MRVEIWSDVICPWCYIGKARFEKGLAAFAGRDEVEVVHRSFELSA
                     GAARGGRQELGPELKAKYGWDDAQLKAMEDRVGAAAAAEGLGMRHDRFVTGTFDLHRVL
                     HLAKERGLQDRLLDAFYTANFAEARPISEDDTIVDIATGAGLDAGEVRRVLADEDAYAD
                     AVRADEKEAAELGATGVPFFVIDRRFAVSGGQPAQVFTQALEQAAAAGGLQTVAGTGGD
                     GAVCADDDACDVP"
     gene            complement(7204..7908)
                     /locus_tag="DMB38_RS05535"
                     /old_locus_tag="DMB38_05550"
     CDS             complement(7204..7908)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1101: phosphoglycerate mutase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835752.1"
                     /locus_tag="DMB38_RS05535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_05550"
                     /product="histidine phosphatase family protein"
                     /protein_id="WP_125938826.1"
                     /transl_table=11
                     /translation="MLVTPAVNAELRHAVFGADEPLDAAGRRAAERAGAHAAASLRTHT
                     PAYAAPSVRCRQTADALGLAAEPEAELADWDVGRWRGRGLEELAAAEGEAVRAWLTDPA
                     AAPHGGESLLQLRGRVGVWLDGLTGPAGTAGPAGTAGPAGTAGTAGTAGTETTGDTGDT
                     HRTDSAPAPALTHRVVAVAEPAAVRAAVVHALDLPSAAFWRLDVPPLTRTELTGRAGRW
                     NLRCGIPLTPDP"
     misc_feature    complement(7897..7912)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.26"
                     /tool="antismash"
     regulatory      8031..8113
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /regulatory_class="riboswitch"
     gene            8173..8388
                     /locus_tag="DMB38_RS05540"
                     /old_locus_tag="DMB38_05555"
     CDS             8173..8388
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007451619.1"
                     /locus_tag="DMB38_RS05540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_05555"
                     /product="CbtB-domain containing protein"
                     /protein_id="WP_125934197.1"
                     /transl_table=11
                     /translation="MAQSAVSPTTDSLLTPVPLRTIAPWALFGGILMLVLPYVVGAGQG
                     AGSLVPGEGVREWLHDGRHLLGFASR"
     gene            8719..>8967
                     /locus_tag="DMB38_RS05545"
                     /old_locus_tag="DMB38_05560"
                     /pseudo=""
     CDS             8719..>8967
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA]"
                     /GO_process="GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835750.1"
                     /locus_tag="DMB38_RS05545"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_05560"
                     /product="cobalamin biosynthesis protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTPDTASHPARLIGGRPLAYALGATLGYAADHLFGDPRRAHPVAA
                     FGRAAAALESRLWRDNRAAGVLHTALCVGATVATAALA"
     gene            9430..10329
                     /locus_tag="DMB38_RS05550"
                     /old_locus_tag="DMB38_05565"
     CDS             9430..10329
                     /GO_function="GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA]"
                     /GO_function="GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA]"
                     /GO_process="GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835750.1"
                     /locus_tag="DMB38_RS05550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DMB38_05565"
                     /product="CobD/CbiB family cobalamin biosynthesis protein"
                     /protein_id="WP_240662672.1"
                     /transl_table=11
                     /translation="MGEHTPLTAPAGPATRRSPQTTPATTTPGPGPRSPEGPTGAAPAG
                     PWGRSPRGSGRGGIGEQVPFVLLVAFAAWAVLGGASLRREALGIAHALRAHDLADARHR
                     LPHLCGRDPHRLGEADIARAVVESVAENTSDAVVGALLWGAVGGVPGLAGFRAVNTLDA
                     MVGHKSARYLRFGWAAARLDDLAGYPGARLTAALAALAGPDPRAARRVWREDGPRHPSP
                     NAGPVEAAFAGALGLRLGGELSYAGRTEHRAVLNPAGRAPGVHDIERAVRLSARVSFLA
                     LLTTTLAAALLPTEASHA"
ORIGIN
        1 gggtgccggg tgggccaggt gtgggggacg cactggcacg gggccctgga gaacgacggc
       61 ttccggcggg cgttcctgcg gcaggtggcg gcggacgccg ggcgggcgtt cgtgccggcg
      121 ccggacacgt ccttcgcggc actgcgggag gagcagttgg agaagctggg cgacctgatc
      181 gaggagcacg cggacaccaa ggccctgctg cgcctgatcg agtcgggcgc cccgggcgac
      241 ctgccgttcc tgccgcccgg ggcgccgacg gcgtagcggc cgtaccggca cgcgggggac
      301 gtccccggta ccggtacggc cgtcagacac ggtgccgcac gcgcgccgct ctcgcgcgtg
      361 tcagccccgc gtccggatgc gggccagcac caggccgctg aggcccaggc cgacgaggag
      421 ggcggcgccg ccgaccaggt acggggtcgc cgagctgccg cccgtttctg cgagttgccg
      481 ctcgggggcg gggtcggtgg cgtcggcggg ccgctcgtcc tggaccggct cggcgtcgcc
      541 ctgggggcgg tcgccgggct cctcccccgg gcccgtaccg ccggcaggcg tctcgcacgc
      601 cgcctcgacc agcgtcacct cgccctccac ctcggcgacg ttcagcttca gcgggttcac
      661 cgagacccgc agccgcagcg cggcggcgct cgccgccgcg cgcgtggtct ccgtctgcga
      721 gaagccgagc gacacctccc ccacgcccgg caccgcgacc accgtcggct cgccgcccac
      781 ggtgaccgtg acgcgcttgc cgaggacggt gacggagccg agggtgcggg actccgcctt
      841 cggcttctcc cccaccgcgc agacggcctt cgacgtcacc tcctcgacct tgatgagcgg
      901 gagcgccggc agcccgggga cgtgcacctc ggcgcgggcg aggtgcgacg acgcctcggc
      961 gccgtacacg tccacggtgg cgtccgcggt ggcgacttcg gcgcgcgcga tgttcaccgc
     1021 ccggggtcct tccgggtgtt cgccgccctc ggcggccgcg aggccgtcga cccggacgtc
     1081 gagcgcggtc ttgtccgcgg caccggagtc ccggcccgcg gcgggcaccc ggacctcgtt
     1141 gagcgtcgaa agcaccggca cccgcactgt cttgtcgagc agggagacgt cgaggtcggc
     1201 gcggagcacg gaggcgccgg ccgagccggt acgggccggg gccgcgccgt cggcgccgtc
     1261 ggcgtggacg ggtgcggcaa ggagcagggc cgggccggcg gccagtgcgg ccgccgtgac
     1321 ggcggtcgcg ggcatgcgaa aaaggcggga caagcgggac aaggaacaaa ccccccacgg
     1381 gtttacgtgc gtcggatcgt caactgcccc gccagcttta cgcactcagg gtgacgcgac
     1441 ggacactctg cgctcgttca ccccttcggg ggaatcagat cgcggaagtc gccccgcgcc
     1501 ccccgctcct ctgctctcag ccgacgaccc gcccgcgcag gacgatccgc cgcggcgccg
     1561 tcaacacccg cacgtcggcc agcggatcgg cctcgtacac cacgagatcc gcgggggcgc
     1621 cctcctccag gcccgggcgc ccgagccact cccgcgcgcc ccacgccgcc gccgagacgg
     1681 cgtccgccgc cgggatcccc gcggcgacca gctcgcccac ctcctgcccg accaggccgt
     1741 gcgccagcga cccgccggcg tcggtgcccg cgtagacggg gatgccggcg tcgtaggccg
     1801 cgcgcacggt ggcgtaccgg cggtcgtgca gccggcgcat gtggtccgcc cagcggggga
     1861 acttggcctg gccgcccgcc gccagctccg ggaaggtcgc gatgttgacg agggtgggca
     1921 cgatggcgac gccgcgctcg gcgaagagcg gcacggtctc ctcggtgagg cccgtggcgt
     1981 gctcgatgca gtcgatgccc gcctcgacca ggtcgcggag cgagtcctcg gcgaagcagt
     2041 gggcggtcac gcgggcgccg aaacggtgcg cctcggcgat cgcgggggcc agggcctcgc
     2101 gcggccagca cggggtgagg tcgccggcct cgcggtcgat ccagtcgccg acgagcttca
     2161 cccagccgtc gcccctgcgc gcctcgcggg cgacgtacgc gacgaggtcg tccggttcga
     2221 tctcgtgggc gtagttgcgg atgtagcggc gggtacgggc gatgtggcgg ccggcgcgga
     2281 cgagccgcgg caggtcgtcg cggtcgtcga tccagcgggt gtcggcggcg gagcccgcgt
     2341 cgcgcagcag cagggcgccg gcctcgcgct cggtacgggc ctgcttctcg ctggtggcgt
     2401 cgtccaccgc gccgtgcgcg tcgagcccga cgtggcagtg ggcgtcgacg agcccgggca
     2461 gcgcccagcc ctcgatgtcg acgacctcgc gcgcggcggc gggcggcgtg aaggtgacgc
     2521 gcccgcccac cacccacagc tcgtcgcgga cttcctcgcg gccggtcagt acccggcccc
     2581 tcacgcgcag caccgggctc tcgctcatgc cgggcaccct agcgggcggc cgttcgcggc
     2641 gatctccggg gcgtaccaca gccgagctca cgcgaaggaa cgaacacggg gggtgaggca
     2701 ttgacgtatg tttaacggca gtttatcgtc gcctccgcga agtgattttc ccgtcttctt
     2761 ctgcccgctt cccggtcgga taaaccaggt aatttttcgg ccagtcaacc ttctcaatcc
     2821 ggacaccggc cggagtcgtt atcaatacgt tacatttcgg gaccgggcgc cgactccccc
     2881 cgggaattcg ctgatatatc ggggcatatc cccttcggtt cgcgcgctcg cgcgtgcccg
     2941 cggataacac acctacgtcc cttttgtaac ccctaccggc cgtgcgattt caatttcccc
     3001 taggtaaatt caatctgcat gactgccgca caagcagacc tagcaggccc tcccgataaa
     3061 ccccgcaccg cggctgcaga cgaggcgggt gtccggctgg acaccccgcg aatcgaggat
     3121 ggcgccgcaa tctggcgcat cgcacgcgac tccggatcgc tggacctcaa ctcgtcgtac
     3181 agctacctgc tgtggtgtcg cgacttcgcc gacaccagcg tcgtggcacg ggactcggac
     3241 ggcgccccgg tcggcttcgt caccggctat atccggcccg cgcggccgga gaccctcgtc
     3301 gtgtggcaga tcgccgtgga cgccgcgttc cgcggccgcg gcctggcagg cgcgctcctc
     3361 gacgggctca ccgagcgcgc gctggcggac cgcggcatac gccgcctcga aacgaccatc
     3421 acgccggaca actccgcctc cgaccagctc ttccgctcgt acgcgcgccg ccacggcgcc
     3481 ggcgtcgacc gcgacgtgct gttccagggg gagcacttcc cggacgggca cgagcccgag
     3541 ctgctgtacc gcatcggccc gctcgcgggc tgagccgacc gacccattcg caccccccgc
     3601 acaccggatc atctcccagg agaacgctgt gaccatcaca ccgcccgccc tgagcgtctt
     3661 cgagaccctg gagtcggagg tgcgcagcta ctgccgtggc tggcccgcgg tcttcgaccg
     3721 ggcccagggc agctatctgt acgacgagga cggccacacc tacctcgact tcttcgccgg
     3781 cgccggatcc ctcaactacg gccacaacaa cgccgtgctc aagcgcgcgc tcctcgacta
     3841 cctggagcgc gacggcatcg tccacggcct cgacatggcc accaccgcca agcgcgcgtt
     3901 cctggagacc ttccagaacg tcgtgctgcg cccccgcgac ctgccgtaca aggtcatgtt
     3961 cccgggcccg accggcacca acgccgtcga ggccgcgctg aagctggccc gcaaggtcaa
     4021 gggccgcgag gcgatcgtct cgttcaccaa cgccttccac ggcatgtcgc tcggctcgct
     4081 cgccgtcacc ggcaacgcgt tcaagcgcgc cggcgccggc atcccgctgg tgcacggcac
     4141 ccccatgccg ttcgacaact acctcgacgg ccaggtcccg gacttcctgt ggttcgagcg
     4201 gctgctggag gaccagggct ccgggctgaa caagcccgcc gccgtcatcg tcgagaccat
     4261 ccagggcgag ggcggcatca acgtggcccg cgccgactgg ctccgcggcc tcgccgagct
     4321 gtgcaaacgc cgcgacatgc tgctcatcgt cgacgacatc cagatgggct gcggccgcac
     4381 cggcgggttc ttctccttcg aggacgcggg catcacgccc gacatcgtca cgctgtcgaa
     4441 gtcgatcagc ggctacgggc tgccgatgtc gctcacgctc ttcaagccgg agctggacat
     4501 ctgggagccg ggcgagcaca acggcacctt ccgcggccac aacccggcgt tcgtcaccgc
     4561 caccgcggcg ctcgacacgt actgggccga cggccagatg gagaagcaga ccctcgcccg
     4621 cggcgcccag atcgagcagg cgctggtcgc gatctgcgcg gagcactccg acgcggacgc
     4681 cacataccgc ggccgcggca tggtgtgggg gctcgagttc cccgacccgg cgcgcgccgc
     4741 gcgtgtctgc cggcgcgcct tcgagctggg gctgatcctg gagacctccg ggccgcagag
     4801 cgaggtcgtc aagctgctgc cggcgctgac gacgacgccg gacgacctgg acgaggggct
     4861 gcgcacgctg gcccgggcgg tccgcgagac cgcctgaggg ccggggcggc ccgcccgtcg
     4921 cgtacgagca ggcacgtaca cgccggcaga cagagcggca gagccgccga ccgatcccga
     4981 gagagaggca cagaggcacc gtgatcgtcc gatccttcaa ggagcttgaa ggtaccgacc
     5041 ggcacatcaa gtccgcgtcg ggcacctggg agagcaagcg catcgtgctc gcgaaggagc
     5101 gcgtcggctt ctccctccac gagaccgtgc tctacgcggg caccgagacg tcgatgtggt
     5161 acgcgaacca catagaggcc gtcgtctgcg tcgagggcga agccgaactc accaacgacg
     5221 agaccggcga gaagcacgcc atcacgccgg ggacgatgta cctgctggac gggcacgaga
     5281 ggcacacgat gcggatcaag gaggacttcc gctgcatctg tgtcttcaac ccgccggtca
     5341 cgggccgcga ggaccacgac gagaacggcg tgtacccgct gctgactgag gaggactgag
     5401 aaccatggcc agcccgactg tcaccgacct ctacccgacg cgcggcgcgg aagaagtgag
     5461 cacgccccgt caggacccgg tggtctggtc ggaaccgggt gccgacgggc ccatcccgcc
     5521 ctgggagctg gagggctacg agcgcaacgg ctacctgacg gtcgaccagc tgatcacccc
     5581 ggaggaggtg gagacgtaca aggccgagct ggaccggctc atcgccgacc cgcgggtccg
     5641 cgccgacgag cgctcgatca tcgaggcggg cacccaggcc gtacgctcca tcttcgagat
     5701 ccacaagatc agcgacgtct tcggcaagct ggtacgcgac gagcgcgtcc tggagcgggc
     5761 gcggcagatc ctcggctccg acgtctacat ccaccagagc cggatcaaca tcaagcccgg
     5821 gttcggcgcc agcggcttct actggcactc ggacttcgag acctggcacg ccgaggacgg
     5881 cctgccgaac atgcggaccg tgtcggtctc gatcgcgctg acggagaact acgagaccaa
     5941 cggcggcctc atgatcatgc cggggtcgca caagacgttc ctcggctgcg cgggtgccac
     6001 gccgaaggac aactacaaga agtccctgca gatgcaggac gccggcacgc cgtcggacga
     6061 ggcgctgacg gagatggcgg aggcgcacgg catcaagctg ttcaccggcg cggccggcgg
     6121 tgcgacctgg ttcgactgca actgcatgca cggctcgggc gacaacatca cgccgtaccc
     6181 gcgcagcaac gttttcctcg tcttcaacag cgtggagaac gcggcggtgg agccgttcgc
     6241 ggcaccggtg cggcggccgg acttcatcgg cgcgcgggac ttcacgccgg tgcggtgacg
     6301 cggagcgcga tggccgtggc gtacctccgc ggacctgcgg ggatgtgaca ccgggacggt
     6361 gccgcgcccc ccgcccggat ccttccgggc ggggggcgcg gccgtgttcc gcgggactgg
     6421 gctcctgggg ggccggtccg ctacgggacg tcgcaggcgt cgtcgtcggc gcagacggcg
     6481 ccgtcgccgc ccgtgcccgc gacggtctgc agcccgcccg cggcggcggc ctgctccagg
     6541 gcctgcgtga agacctgcgc cggctgcccg ccggagacgg cgaagcggcg gtcgatgacg
     6601 aagaacggca caccggtggc gcccagctcg gcggcttcct tctcgtcggc gcgtacggcg
     6661 tcggcgtagg cgtcctcgtc cgcgagcacc cggcgcacct cgccggcgtc gaggccggcg
     6721 ccggtggcga tgtcgacgat cgtgtcgtcc tcactgatcg ggcgggcctc ggcgaagttg
     6781 gcggtgtaga acgcgtcgag gagccggtcc tgaaggccgc gctccttggc gagatgcagg
     6841 acgcggtgca ggtcgaaggt gccggtgacg aaccggtcgt ggcgcatgcc gagcccctcg
     6901 gccgccgcgg cggcgccgac ccggtcctcc atcgccttga gctgcgcgtc gtcccagccg
     6961 tacttggcct tcagctcggg gccgagctcc tgccggccgc cccgcgcggc accggcggag
     7021 agctcgaagg agcggtgcac cacttcgacc tcgtcccggc ccgcgaaggc ggcgaggccc
     7081 ttctcgaacc gggccttgcc gatgtagcac caggggcaga tgacgtcgga ccagatctct
     7141 acacgcatga cggcggaaac ggacggggtg gtggcgggta ttccccgggt caggggtccg
     7201 gggtcagggg tccggggtca gggggatgcc gcaacggagg ttccagcggc cggcgcggcc
     7261 ggtcagttcg gtgcgggtca gcggcggtac gtcgaggcgc cagaaggcgg cggagggcag
     7321 gtcgagggcg tgcacgacgg cggcgcgtac ggcggcgggc tcggcgacgg cgacgacgcg
     7381 gtgggtgagg gcgggggctg gggcgctgtc cgtccggtgc gtgtcgccgg tgtcgccggt
     7441 ggtctccgtt cctgccgtgc ccgcggtgcc cgcggtgccc gccggtcctg ccgtgcccgc
     7501 cggtcctgcc gtgcccgccg gtccggtcag gccgtcgagc catacgccga cccgcccccg
     7561 caactgcagc agcgactccc caccgtgcgg ggccgccgcc ggatccgtca gccaggcccg
     7621 taccgcctcg ccctccgccg ccgccagttc ctccaggccc cgcccgcgcc agcggccgac
     7681 gtcccagtcc gccagctccg cctcgggctc cgccgcgagc cccagcgcgt cggccgtctg
     7741 ccggcagcgg accgacggcg cggcgtacgc cggcgtgtgc gtacgcagcg aagccgccgc
     7801 gtgggccccc gcgcgctccg ccgcacgtcg acccgccgcg tccagcggtt cgtccgcgcc
     7861 gaacaccgcg tgccgcagct cggcgttgac cgccggcgtg acgagcatca cccggaccgt
     7921 caccagcagt ctcacctgcc ccttccgcat ctcggccggc ggacgccctg tggtcgccgg
     7981 cccgcaggcg acggtatcgt cacctcgtca accgaagacg acggcgcaac ggaagcccgg
     8041 tgagattccg gcacggccgc gccactgtga gccggacgac gccccgcgcg gggcggagtt
     8101 cggtgagtca gacccgcacc gtcgtgaaga gctccaccgg atgggacgcg tgatccccgg
     8161 gaggtttccg ccatggctca gtcggccgtg tcacccacga cagactctct cctcacccct
     8221 gtgccgctga ggaccatcgc cccgtgggcg ctgttcggcg ggatcctgat gctcgttctg
     8281 ccgtatgtcg tcggtgccgg acagggtgcc gggtctctgg tgccgggcga gggcgtgcgg
     8341 gagtggctgc acgacggccg tcacctgctg ggattcgcct ctcggtgatc acactgaact
     8401 ctgcggcggt gcgtggggtg ttgctgcgcg ggatgccggc ggggccgggt gcgggggtgg
     8461 cgggctgagg ggggttgttc cccgaccccg ccccttcccg aaactcgggg gctccgcccc
     8521 gagaccccgc cggggctgcg cccctgaccc cgcccggggc tacgccccga accccgcggg
     8581 gcttgtgccc ccagaccgca gacggccacg tatcccgccc gatcgccggg ctgtcgcccc
     8641 gcccctttcc cgggggcttc gcccccggaa cccgaccacc gaatgcccac cggcacacgc
     8701 cccgcaccga acggcaccat gacacccgac accgcgtcac accccgcccg gctcatcggc
     8761 ggccgcccgc tggcgtacgc cctgggcgcc accctcggct acgccgccga ccacctcttc
     8821 ggcgaccccc gccgcgccca cccggtagcg gccttcggcc gcgccgccgc agcactcgaa
     8881 tcccgcctct ggcgcgacaa ccgcgcggcg ggcgtcctgc acacggcact ctgcgtaggc
     8941 gcaacggtgg cgacggccgc cctggccacg gcagcaacgc gcagggcgac agccccgggt
     9001 tggggccaga gcacgtggac ggcgaggcgc agcctcgacg cacgcacggg catggagcgg
     9061 gacccgggct cggggcacag ccccgcggga cgttcggaca cagccccggg cgggagttcg
     9121 gcgctgggtc ccggaggggg caggggcgag ggcacgaggc ccggtcgccg cgcccccgcg
     9181 ggcgcagccc cagcggggtc ttgggggcgc agtccccgag gtccgggaag ggtcggggag
     9241 cacacccccc tcacgaccca gccggacctc accgctgcca cagccgcacc gggaccccaa
     9301 gggcgaagcc cggaggggca cgcgggcgca ccccggtgcc cgggttcggg gcgcagcccc
     9361 gagggggcca ggggcgcagc cccggcgggg tcttgggggc gcagccccca agtttcggga
     9421 aggggcggga tcggggaaca cacccccctc accgcacccg cgggcccggc cacgaggcgt
     9481 agcccccaga cgaccccggc cacaaccacg ccagggcccg ggccgcgaag ccccgagggc
     9541 cccacgggcg cagccccggc ggggccttgg gggcgcagcc cccgaggttc gggaaggggc
     9601 gggatcgggg aacaggtccc gtttgttctg ctcgtcgcgt tcgcggcctg ggccgtcctc
     9661 ggcggagcct ccctccgccg tgaagccctc ggcatcgccc acgccctccg cgcccacgac
     9721 ctcgccgacg cccgtcaccg cctcccccac ctctgcggcc gcgaccccca ccgcctcggc
     9781 gaagccgaca tcgcccgggc cgtcgtcgag tccgtcgccg agaacacctc cgacgccgtc
     9841 gtcggcgccc tcctctgggg ggcggtcggt ggcgttcccg ggctcgccgg gttccgggcc
     9901 gtcaacaccc tcgacgccat ggtcgggcac aagtccgccc gctatctgcg cttcggctgg
     9961 gccgccgccc ggctcgacga tctcgccggc tatcccggcg cgcggctcac cgccgcgctc
    10021 gcggcgctcg ccgggcccga cccccgcgcc gcccggcgcg tgtggcgcga ggacgggccg
    10081 cggcatccca gccccaacgc aggacccgtg gaagccgcgt tcgccggtgc gctcgggctg
    10141 cggctcggtg gggagctgtc gtatgcgggg cggaccgagc accgggccgt gctcaatccg
    10201 gccgggcgcg ctccgggcgt gcacgacatc gagcgtgccg tccgcctctc cgcccgtgtc
    10261 tccttcctcg ccctcctcac caccaccctc gccgccgccc tcctccccac cgaagcgagt
    10321 cacgcatgac caccgtcctc ctcctctcca ccgccgacac cgacctcctc gccgcccgcg
    10381 cctccggcgc ggacttccg
//
