LOCUS       NZ_RHGC01000004        10441 bp    DNA     linear   CON 30-MAR-2024
DEFINITION  Streptomyces alboflavus strain 313 contig004, whole genome shotgun
            sequence.
ACCESSION   NZ_RHGC01000004
VERSION     NZ_RHGC01000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces alboflavus
  ORGANISM  Streptomyces alboflavus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 893660)
  AUTHORS   Guo,Z., Li,P., Chen,G., Li,C., Cao,Z., Zhang,Y., Ren,J., Xiang,H.,
            Lin,S., Ju,J. and Chen,Y.
  TITLE     Design and Biosynthesis of Dimeric Alboflavusins with Biaryl
            Linkages via Regiospecific C-C Bond Coupling
  JOURNAL   J Am Chem Soc 140 (51), 18009-18015 (2018)
   PUBMED   30516971
REFERENCE   2  (bases 1 to 893660)
  AUTHORS   Guo,Z., Li,P. and Chen,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2018) State Key Laboratory of Microbial Resources,
            The institute of Microbiology, Chinese Academy of Sciences, No. 1
            Beichen West Road, Beijing 100101, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 10x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003935055.1-RS_2024_03_29
            Annotation Date                   :: 03/29/2024 13:06:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,631
            CDSs (total)                      :: 7,558
            Genes (coding)                    :: 7,426
            CDSs (with protein)               :: 7,426
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 3 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 40 of 132
            Pseudo Genes (incomplete)         :: 103 of 132
            Pseudo Genes (internal stop)      :: 12 of 132
            Pseudo Genes (multiple problems)  :: 20 of 132
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:58:57
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 127398
            Orig. end                         :: 137839
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            RHGC01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10441
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="1"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10441
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10441
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5441](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5441)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            704..1654
                     /locus_tag="EJ355_RS16720"
     CDS             704..1654
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013002130.1"
                     /locus_tag="EJ355_RS16720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="L,D-transpeptidase"
                     /protein_id="WP_193560685.1"
                     /transl_table=11
                     /translation="MARRTRIRHVVGGAAAAVLCAGVLAGCSLGGQDGASAPSERRDDR
                     KAAGAGTPTPRAKPVVEPAALRGDGVRVNVADGQRVGVGMPVSVTFDRPVPAAERAAVE
                     RRLKVRASTPVTGSWSWVKDRNLHEGQRVDYRPKTYWKPGTKVTVRVGSGIDRSFTVAR
                     SLVATVDVRSHTMTVVKDGVTRRVPITAGAPGMDTWNGTMVVSDKQRRVFMDSRTVGYA
                     DAYADYYSYAVHLTTSGTYLHENPKANTYAGNSNVTHGCIGLATDGTARRFYEEVIPGD
                     VVRVTGSKETVAPGNGYGDWNVPWADWRAGSALNS"
     tRNA            complement(1670..1744)
                     /anticodon=(pos:complement(129107..129109),aa:Cys,seq:gca)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="EJ355_RS16725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /product="tRNA-Cys"
     gene            complement(1670..1744)
                     /locus_tag="EJ355_RS16725"
     gene            1912..2463
                     /locus_tag="EJ355_RS16730"
     CDS             1912..2463
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027: isochorismatase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018562509.1"
                     /locus_tag="EJ355_RS16730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="isochorismatase family protein"
                     /protein_id="WP_125263264.1"
                     /transl_table=11
                     /translation="MTTLPDRPRTAVLVIDMQNGVVAEAHERDTVIANIVTVVDKARAA
                     DVPVIWVQHSDDELPRGSESWAYVPELVRRDGEPLVHKLYGDSFEATDLESVLAAHAVG
                     HLVVTGAQTDACIRSTLHGAFTRGYDATLVADAHTTEDLSAYGAPTPDLVIAHTNLYWH
                     RQEAPGRRGGTLRAQELSFS"
     gene            complement(2474..2893)
                     /locus_tag="EJ355_RS16735"
     CDS             complement(2474..2893)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016327038.1"
                     /locus_tag="EJ355_RS16735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding protein"
                     /protein_id="WP_125263265.1"
                     /transl_table=11
                     /translation="MDSETTQVPAAVRTFSIRLSSTLRGARLARRAAVTQLHVWGLPFE
                     PAQHIVAELASNAVRHGYVPGRDFRLTLRLTEGGVLRVEVTDTYTERLPRPQGRVAPEA
                     ESGRGLLLVEALATRWGTEVGPAPGKTVWAAIGAV"
     gene            3053..3908
                     /locus_tag="EJ355_RS16740"
                     /pseudo=""
     CDS             3053..3908
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019326171.1"
                     /locus_tag="EJ355_RS16740"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSTDGEGVDETGWDVEPGDEIASVVEALGHQQKRWREAAGLSAPE
                     FGEAIGYGEDLVRKVERGGAYPAPGVLGCRRPCAGGGRLHLGDEGGDGQGAVPEEGSGA
                     GEVGGAGGRVVPVQHS"
     gene            3905..4174
                     /locus_tag="EJ355_RS16745"
     CDS             3905..4174
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005310395.1"
                     /locus_tag="EJ355_RS16745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF397 domain-containing protein"
                     /protein_id="WP_087884314.1"
                     /transl_table=11
                     /translation="MTRKPCAGGSGQLHWFKSSYSDSSNPSDCVEVALEWTKSSYSSSS
                     NGEDCVEVAPTPTTIHVRDSKNRQGPHLGFAAGAWRDFVQAATS"
     gene            complement(4156..4983)
                     /locus_tag="EJ355_RS16750"
     CDS             complement(4156..4983)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038020.1"
                     /locus_tag="EJ355_RS16750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase family protein"
                     /protein_id="WP_087884313.1"
                     /transl_table=11
                     /translation="MTVRKNQSQLTADEKRRFVTALLALKRDGRYDSFVSTHNDFIMSD
                     TDNGDRVGHRSPSFLPWHRRFLIQFEQALQSVDASVALPYWDWTVDRTPAASLWAPDFL
                     GGTGRSRDSQVTDGPFASAGNGWRINVRVDGRDFLRRSLGAGVRQLPTKAEVDSVLAMS
                     TYDMPPWNSASDGFRNHLEGWRGVNLHNRVHVWVGGHMATGASPNDPVFWLHHAYVDKL
                     WTQWQSRHPGSPYLPAAGTRDVVDLNDTMRPWGDVTPADMLDHTQYYTYDVAA"
     gene            complement(5001..5441)
                     /locus_tag="EJ355_RS16755"
     CDS             complement(5001..5441)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018848222.1"
                     /locus_tag="EJ355_RS16755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase cofactor"
                     /protein_id="WP_087884312.1"
                     /sec_met_domain="melC (E-value: 2.8e-45, bitscore: 149.5,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGITRRRAIGAAASAAAGIAVVGAARAAATAAEPGAEAHPGAAP
                     DAFDEVYLGRRIQGAPSHGGGHHGAHGGHGAGYSVRIDGDELHIMRNADGTWISVINHY
                     EPRATPRALARAAVVELQGATLVPLAPPAHSSPTAPSSPSAV"
     misc_feature    complement(5605..5620)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.86"
                     /tool="antismash"
     misc_feature    complement(5660..5675)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.87"
                     /tool="antismash"
     gene            5851..6567
                     /locus_tag="EJ355_RS16760"
     CDS             5851..6567
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EJ355_RS16760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_125263266.1"
                     /transl_table=11
                     /translation="MTSTPPMPPTPPQPYAQYPQPPQPADGRRGRTVAVVVVVVLAVLA
                     AGLGTWWWTRDGDEGPLAGRPRVTDARAGMSYGIPEGWERNDKKGDLIGAFTSSIASKG
                     KGLADSAVEGSEEEGGGIVLAGRSGAVPESSLKTETERAARSNAEFFYPDGSSEVKESE
                     ATTVDGRPAHTTALTVRDGEGGTAHLRLTLVSVDGTRSAFLLGVATPGTDIGPYADKGP
                     VDEKVVDEVVASVELN"
     gene            6676..7572
                     /locus_tag="EJ355_RS16765"
     CDS             6676..7572
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EJ355_RS16765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_087884310.1"
                     /transl_table=11
                     /translation="MTQPPGQRPGQPQSQPWQGGGFGPPPGPAPGTQGTYAPPPQGSCG
                     AQQPAADNPYAQPGRQPATHPNAQPSHGGYPPHSPQFPTPDSGGPKRGRWLVVTAAVVA
                     LAVVGVGVWFAVGKGDDDGESKTPAAKASRSAGAEPRPSETPSAQESDDAGESDDPYAD
                     PDAGEDEDEPGGGPTGTTLVGLWRSEKPGGGILGLRDSPDTTKPKRASVSLLDEGRCSG
                     LRRVIEPGRSYRIGLLCERDKKEIFGDLIFPGGDTLTVTWDKGRSGTETYKRFMDWSED
                     GTGAGPGTGGDPGSEGV"
     gene            7722..8177
                     /locus_tag="EJ355_RS16770"
     CDS             7722..8177
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004985475.1"
                     /locus_tag="EJ355_RS16770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_125263267.1"
                     /transl_table=11
                     /translation="MVWHEWEQLKAEAAERHSPAMQIDSTPKGDDGGGGGPWGGLRSSK
                     AAWTKAGDGVGGLKEGIGRALAKLEDGQAGLGGLDGCQSAAAQREVHRSWERYVKDVSK
                     RCGSLGDLLAKMGSDQQKTDDAVRVEIEKLKDLYADTPDLGGQSKGR"
     gene            8186..9988
                     /locus_tag="EJ355_RS16775"
     CDS             8186..9988
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018518840.1"
                     /locus_tag="EJ355_RS16775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_107490127.1"
                     /transl_table=11
                     /translation="MDYATLKSLKPSEFEDAADGYRATSDMASAAKSRIENQVAPGMRS
                     SLEGKTLEAALKQLKALTENFHYTQTECGLVATALNGLAHDLASAKRKLDSAVSDAQAE
                     KFTVNPDGSVTYPAAGEKTDGKLPEGGTAKGTADGTAADVYRQSANFNPNPNFGRAQEY
                     ANRIAIALQEATEADEKWAPKLRRLKADDDLTVSHRDWGDVQSDTRVVGTGAKEYLESA
                     GGPPKDGTPQDNAKWWKGLSDQERDAYVALRPASVGALDGLPADVRDEANRTVLAEKRA
                     QFQLELNSFPPMPMKYQPPTSGTTKKYREWVEKTGGEDRIEFLQESIKGMDAIQGRFDR
                     TGSGKNPLPQAYLLGFDPKGAGDGRVIIANGNPDTADHTSVYVPGTKASLSNIDGDIGR
                     SETLWGAASRKVPGQDVSTITWLDYDTPRSAIPFVDGDIDPEAAYPGRAEKAGPTLSQF
                     MHGVQEAQGGPTASHTTLVGHSYGSTAIGEASKNGGLAADDVLVAGSPGMQVKHADDLD
                     VQKGHVWAMGAPTLSDPVPLGGKLVGLGADWNVPTDDDFGANIMKTDSSSHSGYWDTSD
                     RKPSLSLMNQASVIAGKYDEVKLD"
     misc_feature    8747..8749
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(10197..10213)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 19.72"
                     /tool="antismash"
     misc_feature    10200..10213
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.26"
                     /tool="antismash"
ORIGIN
        1 ccgagtacgc cgtgcgcacc gccgacgccg ccgctgtcgc ccgcgtcgcc cccgtactcc
       61 ccggacaaat gcttggccag cgccgcgcgt ccgcgcgaga gccgcgcctt gacggtgccg
      121 gtcggggcgc cggtctcgga ggctacctgc tccacgctga ggtcacacag gtggtgcagc
      181 acgatcgcga gccgctgtgc ctcgggcagc ttccgcagcg ccgcgacgag cgtcacgtgc
      241 tccggccccg gcccggccgc gtgctcgggc ggcggctcct tgcgcaccag ctccagccag
      301 cgccgggccc gccgccagtg gctcaccgcg agccgcatgg ccaccgtgcg gacccaggcc
      361 tccggcgcgt gcacggcggc gaagtcgcgc cgccggtccc aggcgcgtac gaacgcctcc
      421 tggacgacgt cctgggcctg accgtggtcg ccggtgaagg cgtagacctg gccgacgacg
      481 cgcgggaacg cggacgcgta gaacgcgtcg aactcgtcct cggtcatgcc cccgccgttc
      541 ctgtccacga ccgcgaccgt tcgccgcccg cgccgcgccc gtcacaacca gccccgaatt
      601 tcttcccgca cgcctcatgc aacccggcgg tctccgccgt gcgtatgggt ggcgtacatg
      661 gaaacacacc ggcccaggcc ggaacaccac acagggggac ccgatggcca ggcgcaccag
      721 aatccgtcat gtcgtaggcg gggcggcggc cgccgtgctc tgcgcgggcg tcctcgcggg
      781 ctgctcgctg ggcgggcagg acggtgcttc ggcgccgtcg gagcggcggg acgaccggaa
      841 ggccgccggg gccggtacgc ccacgccgcg cgcgaagccg gtggtggagc ccgcggcgct
      901 gcgcggggac ggggtgcggg tgaacgtggc ggacgggcag cgggtgggcg tgggcatgcc
      961 ggtgtcggtg acctttgacc gcccggtgcc cgcggccgag cgggcggcgg tggagcggcg
     1021 cctgaaggtg cgcgcgagca cccctgtcac cggttcctgg agctgggtca aggaccgcaa
     1081 cctgcacgag ggccagcggg tcgactaccg ccccaagacg tactggaagc ccggcacgaa
     1141 ggtcaccgtc cgggtgggct ccggcatcga ccgcagcttc acggtggccc gctccctggt
     1201 cgccaccgtc gacgtacgca gccacacgat gaccgtcgtc aaggacggcg tgacgcgccg
     1261 cgtgccgatc acggcgggcg cgcccggcat ggacacctgg aacggcacga tggtggtctc
     1321 cgacaagcag cgccgcgtct tcatggactc ccgcacggtc ggctacgcgg acgcgtacgc
     1381 cgactactac agctacgcgg tccacctcac gacgtccggc acgtacctcc acgagaaccc
     1441 gaaggccaac acgtacgcgg gcaactccaa cgtcacccac ggctgcatag gcctcgccac
     1501 ggacggcacg gcccgccgct tctacgaaga ggtcatcccc ggcgacgtcg tccgcgtcac
     1561 cggctccaag gagacggtgg cccccggcaa cggctacggc gactggaacg tcccgtgggc
     1621 cgactggcga gcgggcagtg ccctcaactc ctgacccctg acggggactt ggaggccgtg
     1681 accggaatcg aaccggtgtg cttcgctttg caggcgaatc cctcaaccac tcgggcacac
     1741 ggccacgtcg tacgctctcg ccgtacgtga cgtatcgacc gtaggcggca cggctcgcgc
     1801 cgatcaagcg ccgggccgtc gccgcaacgc gactgccatg cggcgttcac gaaacaggga
     1861 acgccgcggc agagaacccg tggaccgtgg accgtggaac tggagacccc catgacaacc
     1921 ctgcccgacc gcccccgtac cgccgtgctg gtcatcgaca tgcagaacgg tgtggtggcc
     1981 gaggcgcacg agcgggacac cgtgatcgcg aacatagtca ccgtcgtgga caaggcgcgt
     2041 gcggccgacg tgcccgtgat ctgggtccag cactccgacg acgaactccc gcgcggcagc
     2101 gagagctggg cctacgtccc cgagctcgtc cgccgcgacg gcgagcccct cgtccacaag
     2161 ctctacggcg actccttcga ggccaccgac ctggagtccg tcctcgccgc acacgccgtc
     2221 ggccacctcg tcgtcaccgg cgcccagacc gacgcctgca tccgctccac cctccacggc
     2281 gccttcaccc gcggctacga cgcgaccctc gtcgccgacg cccacaccac ggaggacctg
     2341 agcgcctacg gagccccgac ccccgacctc gtgatcgccc acaccaacct gtactggcac
     2401 cgacaggaag cccccggccg tcgcggcggc accctgcgcg cgcaggagct ctccttctcc
     2461 tgagccgcgc cgttcagacg gcgccgatcg ccgcccacac cgtcttgccc ggcgcgggcc
     2521 ccacctccgt gccccagcgc gtcgcgagtg cctcgaccag gagcaggccc cgccccgact
     2581 ccgcctccgg cgccacccgg ccctgcgggc ggggcagcct ctccgtgtac gtgtccgtca
     2641 cctcgacacg tagcaccccg ccctccgtca gccgcagcgt cagccggaag tcccggcccg
     2701 gtacgtaccc gtgccgcacc gcgttgctcg ccagctccgc cacgatgtgc tgcgcgggct
     2761 cgaacggcag cccccacacg tggagttggg tcaccgccgc tctgcgcgcc aggcgggcgc
     2821 ccctcagcgt cgaggagagc cggatgctga acgtcctcac ggcggccggg acttgcgtgg
     2881 tctccgagtc catgcacctc agcgtgacgc cagccggttc ccctgcaccg tgatgcgtgg
     2941 tgcacgtacg gtgactgtcc cgtcgctgtc cggtgctgtc ccggctgtcc ggagtgcctt
     3001 acgggtacgg ctgcgcgttg gagcggggcg gcgcttttcg gaggtgggcg tgatgagtac
     3061 ggacggcgaa ggtgtggacg agacgggctg ggacgtcgag ccgggggacg agatcgcctc
     3121 ggtggtcgag gcgttgggtc accagcagaa gcggtggcgt gaggccgcgg ggctgagcgc
     3181 gccggagttc ggcgaggcga tcgggtacgg ggaggacctt gttcgcaagg tcgagcgggg
     3241 gggcgcgtat cccgcgcccg gagtacttgg atgccgccga ccgtgtgctg ggggcgggcg
     3301 gcttcatctc ggcgatgaag gaggagatgg ccaaggcgcg gtacccgaag aaggttcggg
     3361 agctggcgaa gttggaggag cgggcggtcg agttgtgcct gtacagcact cataacgtcc
     3421 acgggctgct ccagaccccg gagttcgcgg aggcgctgct gcggacgcgg cgcccggcgc
     3481 tggcccaagg tgagctggag cgtgctgttg ctgcacgaat ggcgcggcaa agcatctacg
     3541 gccgagaacc ggcacctgag ctgagctttg tccaggaaga ggtgactctg cggaggccga
     3601 tcggggggac aatgattctg cgtcggcaac tcgaacacat gctggagatc ggacagttgc
     3661 cgcacgtcga acttcaggtg atgccgacga atcgtggtga tcaccccggg acaggtgggc
     3721 ggattcaggt gctgaagttc gcggacggct ctgcgatcgg ccgcaccgac gacgagttct
     3781 cggggcgacc ggtttccgat ccgaggcaac tccgggtcct tgagctgcgc tatggcatca
     3841 tccgggccag ggctctcacg ccacgggagt cactggactt catcgagcaa gtgctgggag
     3901 agacatgact cgcaagccct gcgccggggg tagtggccag ctgcactggt tcaagagcag
     3961 ctacagcgac agcagtaatc ccagtgactg cgtcgaggtc gcgctggagt ggaccaagag
     4021 cagctacagc agcagtagca atggtgagga ctgcgtcgaa gtcgccccca cccccaccac
     4081 catccacgta cgcgactcca agaaccgcca aggcccccac ctcgggttcg ctgcgggggc
     4141 ctggcgtgac ttcgttcagg cggccacgtc gtaggtgtag tactgggtgt gatccagcat
     4201 gtcggccggg gtgacgtcgc cccacgggcg catcgtgtcg ttcaggtcga cgacgtcgcg
     4261 cgtgcctgcg gcgggcaggt agggcgagcc cgggtgccgc gactgccact gcgtccacag
     4321 cttgtcgacg taggcgtggt ggagccagaa gaccgggtcg ttgggggacg cgccggtggc
     4381 catgtggccg ccgacccaga cgtggacgcg gttgtggagg ttgactccgc gccagccttc
     4441 gaggtggttg cggaagccgt cggaggcgct gttccacggc ggcatgtcgt acgtggacat
     4501 cgcgaggacg gagtcgacct cggccttggt ggggagttgg cggacgcccg cgccgaggga
     4561 gcggcgcagg aagtcgcggc cgtcgacacg cacgttgatg cgccagccgt tgccggcgga
     4621 ggcgaagggg ccgtcggtga cctggctgtc gcggctgcgt ccggtgccgc cgaggaagtc
     4681 gggggcccag agggaggcgg ccggggtgcg gtcgacggtc cagtcccagt aggggagggc
     4741 gacggaggcg tccacggact gcaacgcctg ctcgaactgg atgaggaagc ggcggtgcca
     4801 gggcaggaag gacggcgaac ggtggccgac acggtcgccg ttgtcggtgt cgctcatgat
     4861 gaagtcgttg tgcgtgctga cgaaggagtc gtagcggccg tcgcgcttga gggcgaggag
     4921 cgcggtcacg aagcgccgct tctcgtcggc ggtgagctgg gactggttct tgcgtacggt
     4981 catggcggtg ctgctccggg tcagacggcg gacggggacg agggcgcggt cggggacgag
     5041 tgcgcgggcg gggcgagcgg gacgagggtg gcgccctgga gctcgaccac ggccgcgcgg
     5101 gcgagtgcgc gcggggtcgc ccgcggctcg tagtggttga tgacgctgat ccacgtgccg
     5161 tcggcgttgc gcatgatgtg cagttcgtcg ccgtcgatgc ggacggagta gccggcgccg
     5221 tggcccccat gggcgccgtg gtggccgccg ccgtgggacg gcgcgccctg gatgcggcgc
     5281 ccgaggtaca cctcgtcgaa ggcgtcgggc gcggcgcccg ggtgggcctc ggcgcccggc
     5341 tctgcggctg tcgccgccgc acgggcggca ccgacgacgg cgataccggc ggcggcgctc
     5401 gcggcggcgc cgatggcccg gcggcgggtg attccggaca tgcgtaaagc ccccaagtga
     5461 gcgtggtgaa ggggcggttc cggtggtcgg ccggactgcc caggccatgc gtacaggggg
     5521 aaaggcgcgg cgccgcaatc ggcgggaaga gttgcccctt attaaggaca actgcgggta
     5581 agtagtgcag agttggacga gcgtgattat gctgtccatt tgcgctggcg gcgcgcagac
     5641 cgcaaatcct gcgccttgag tgaggataac gtgaaagatc ttcacccatg cttcacggcg
     5701 ggtgcccctg gtcacagcgg aaagctggcc tgaagacctg ccgcccgagg gctgcacgag
     5761 gctggtgtgt gcgggggatg tgtgggagtg agtagggcgc tgagtaggcg tactcagggc
     5821 ccggcggggc cgggcggcga ggcttggcgc atgacttcga cgcccccgat gccgccgacg
     5881 cccccgcagc cgtacgcgca gtacccgcag ccgccgcagc ccgcggacgg tcgccggggc
     5941 aggaccgtcg cggtcgtcgt ggtcgtggtc ctcgcggtgc tcgccgcggg cctcggcacc
     6001 tggtggtgga cgcgggacgg cgacgagggg ccgctcgcgg gcaggccgcg ggtgacggac
     6061 gcgcgggcgg ggatgtcgta cgggatcccc gaagggtggg aacgcaacga caagaagggg
     6121 gacttgatcg gcgcgttcac gtcgtcgatc gcctcgaagg gcaagggcct cgcggactcg
     6181 gcggtggaag ggagcgagga ggagggcggc ggcatcgtgc tcgcggggcg gtcgggggcg
     6241 gtgcccgagt cctcgctgaa gaccgagacg gagcgggcgg cgcgctccaa cgcggagttc
     6301 ttctacccgg acgggtcgtc ggaggtgaag gagtccgagg cgacgaccgt cgacggccgc
     6361 cccgcccaca cgacggccct cacggtgcgc gacggcgagg gcggcacggc ccatctgcgc
     6421 ctgaccctcg tctccgtgga cggcacccgg tccgctttcc tgctgggcgt cgcgacgccc
     6481 ggcacggaca tcggcccgta cgcggacaag gggccggtgg acgagaaggt ggtggacgag
     6541 gtggtggcga gcgtggagct gaactgagcc gcgcctcaac gcccgcccga ctggctcgaa
     6601 ttgcacttgc aatgagcatg cgtgggccac ttggctggac agtctgttgt ctgagtgacg
     6661 ggggaggaac cacagatgac gcagccgccg gggcagcggc ccggccagcc tcagtcgcag
     6721 ccgtggcagg gcggagggtt cggcccgcct ccgggcccgg ctccggggac gcagggcacg
     6781 tacgcgccgc caccgcaggg ctcgtgcggc gcccagcagc cggcggccga caacccgtac
     6841 gcgcagccgg gccggcagcc cgccacccac ccgaacgccc aaccgtccca cggcggttac
     6901 ccccctcatt ccccccagtt ccccaccccc gacagtggcg gcccgaagcg tgggcgctgg
     6961 ctggtcgtca cggccgccgt ggtcgcgctc gcggtggtcg gcgtcggcgt ctggttcgcg
     7021 gtcggcaagg gtgacgacga cggggagtcg aagacgcccg ccgccaaggc cagccggagc
     7081 gcgggcgccg agccccgtcc gagcgagaca ccgagcgcgc aggagtcgga cgacgcgggg
     7141 gagtccgacg atccgtacgc cgaccccgac gccggggagg acgaggacga gcccggcggc
     7201 ggccccaccg gcaccaccct cgtcggactc tggcgctccg agaagccggg cggcggcatc
     7261 ctcgggctgc gcgacagccc ggacaccacg aagccgaaga gggcgtcggt gtccctgctc
     7321 gacgaagggc gctgctccgg cctgcgccgc gtcatcgaac ccggccgcag ctaccgcatc
     7381 ggcctgctct gcgagcgcga caagaaggag atcttcggcg atctgatctt cccgggcggc
     7441 gacaccctga cggtcacctg ggacaagggc agatccggca cggagacgta caagcgcttc
     7501 atggactggt cggaggacgg tacgggggcc gggccgggaa ctggtggcga cccgggctcg
     7561 gaaggggtct gaggcccacg cgtcattcac gccacctgag tacgcgtact cacgcagcgc
     7621 gcatggactg tcgctagcct cacggcagtt gccgcagtac gtccgcggtg cgcgcagcgt
     7681 gggacgcgct tcgcgcggaa catacaggga gggctctgcc catggtctgg cacgagtggg
     7741 agcagttgaa ggctgaggcg gccgagcggc actccccggc gatgcagatc gacagcacgc
     7801 caaagggaga tgacggcggg ggagggggcc cctggggtgg cctcagatcc agcaaagcgg
     7861 cgtggaccaa ggccggagac ggggtcgggg gtctgaaaga gggcatcgga agggcgttgg
     7921 cgaagctgga ggacgggcag gcgggactcg gcgggctcga cgggtgccag agcgcggccg
     7981 cgcagcggga agtacaccgt tcctgggagc ggtatgtgaa ggacgtgagc aaacgctgcg
     8041 ggagcctcgg ggatctgctg gccaagatgg gcagcgacca gcagaagacc gatgacgccg
     8101 tgagggtgga aatcgagaag ctgaaggatc tgtacgcgga cacgccggac cttggcggcc
     8161 agtcgaaggg ccggtgaccg ggcccgtgga ctacgcgacg ctcaaatccc tgaagccgtc
     8221 cgagttcgag gacgctgcgg atggatatcg tgccaccagc gacatggcca gtgccgccaa
     8281 gagtcgcatc gagaaccagg ttgctcccgg catgcgttcg tcgctggagg gaaagaccct
     8341 ggaggccgca ctcaaacaac tcaaggcgct gacagagaac ttccactaca cccagacgga
     8401 atgcggcctg gtcgccacgg cgctcaacgg gctcgcccac gaccttgcgt cggccaagcg
     8461 gaaactcgac tcggccgtct cggacgcgca ggccgagaag ttcacagtga acccggacgg
     8521 ttccgtgacg tatccggcgg ccggcgagaa gaccgacggc aagctgcccg agggcggcac
     8581 cgcaaagggc acggcggacg gcacagccgc tgacgtctac cgtcagtcgg ccaacttcaa
     8641 cccgaacccg aacttcggtc gtgcccagga gtacgcgaac cgcatcgcca tcgctctcca
     8701 ggaagccacg gaggctgatg agaagtgggc gccgaagctg agaaggttaa aggcggacga
     8761 tgacctgacg gtgtcgcacc gagactgggg tgacgtccag tccgatacgc gtgttgtcgg
     8821 cacaggggcc aaggaatact tggaatccgc gggagggccg ccgaaggacg gtacccccca
     8881 ggacaacgcg aagtggtgga aggggctgag tgatcaagaa cgcgacgctt acgttgcgct
     8941 ccgccccgcg tcggtcggtg ctttggacgg actgcctgca gacgtgcgtg acgaggccaa
     9001 tcgtacggtg ctcgccgaga agagggccca gttccagctg gagctaaatt cctttccgcc
     9061 gatgccgatg aagtaccagc cgccgacgtc cggcacgacg aagaagtaca gagagtgggt
     9121 cgagaagacg ggtggcgagg acaggatcga gtttctgcag gaatccatca aagggatgga
     9181 tgcgatccag ggtcgttttg atcgaacggg ttcggggaag aatccgctcc cccaagccta
     9241 cttgctcggt tttgatccca agggggcggg ggacggtcgg gtcatcattg ccaatggcaa
     9301 tccagatacg gccgatcaca cttctgtcta tgtgccgggg acgaaggcga gcctcagtaa
     9361 tatcgatggg gacattgggc gcagcgagac tctctgggga gcggccagtc gcaaggtgcc
     9421 tggccaagat gtttccacca ttacctggct cgattatgac acccctcgat cggcgattcc
     9481 gttcgtcgac ggagatattg acccggaggc cgcttatcca gggcgtgcgg agaaggcagg
     9541 gccgacgctc agccaattca tgcatggcgt tcaggaagcc cagggtggcc ccaccgcgag
     9601 tcacaccact ctcgtcgggc acagttatgg cagtaccgcc atcggtgagg cgtccaagaa
     9661 tggtggcctg gccgccgatg acgtattggt tgcgggaagc cccggtatgc aggtcaagca
     9721 cgcggacgac ttggatgtgc agaaggggca cgtctgggcc atgggtgctc ccactctgag
     9781 tgacccggta cctcttggtg ggaagctcgt tggactcggc gctgattgga atgtgcccac
     9841 agacgatgat tttggcgcaa atatcatgaa gaccgactcc tcgagtcaca gtgggtactg
     9901 ggatacgagc gaccggaagc cgtctttgag tctgatgaat caagcttcag taatcgctgg
     9961 caaatatgat gaggtgaagc ttgattaggt atgtgtcgtc agtgtttatc gctgttttgg
    10021 cggccgctac gctctcgggg tgttcgggca tggatgaatc agaaccagaa acgcgatcag
    10081 tgaagaaggc agtcgaaagc gctgcgggcc tttccagtca gatcctggac atcattgctt
    10141 tgcgtggcaa ggtgagtgag tcgacccctg ggcctgatcc gtgcgatggg aaagatttcg
    10201 agaaatatta cagggtgtat cacgcctgga gtctccacgg tgtccctgtt ccggatatgg
    10261 agagggcgat ggggcgactt aggagtgaat tgccgaaacg tgggtggaag atcattgatt
    10321 atggtcccga ctccagtaag gcgaagagct tggaacttac ggctgaccat ccgaaaagga
    10381 agttctcagt tgtcattact cttcaggatc gtcggggtcg cagcaaggac ccgtccatga
    10441 t
//
