LOCUS       NZ_RHGC01000002        10228 bp    DNA     linear   CON 30-MAR-2024
DEFINITION  Streptomyces alboflavus strain 313 contig002, whole genome shotgun
            sequence.
ACCESSION   NZ_RHGC01000002
VERSION     NZ_RHGC01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces alboflavus
  ORGANISM  Streptomyces alboflavus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1226588)
  AUTHORS   Guo,Z., Li,P., Chen,G., Li,C., Cao,Z., Zhang,Y., Ren,J., Xiang,H.,
            Lin,S., Ju,J. and Chen,Y.
  TITLE     Design and Biosynthesis of Dimeric Alboflavusins with Biaryl
            Linkages via Regiospecific C-C Bond Coupling
  JOURNAL   J Am Chem Soc 140 (51), 18009-18015 (2018)
   PUBMED   30516971
REFERENCE   2  (bases 1 to 1226588)
  AUTHORS   Guo,Z., Li,P. and Chen,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2018) State Key Laboratory of Microbial Resources,
            The institute of Microbiology, Chinese Academy of Sciences, No. 1
            Beichen West Road, Beijing 100101, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 10x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003935055.1-RS_2024_03_29
            Annotation Date                   :: 03/29/2024 13:06:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,631
            CDSs (total)                      :: 7,558
            Genes (coding)                    :: 7,426
            CDSs (with protein)               :: 7,426
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 3 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 40 of 132
            Pseudo Genes (incomplete)         :: 103 of 132
            Pseudo Genes (internal stop)      :: 12 of 132
            Pseudo Genes (multiple problems)  :: 20 of 132
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:58:57
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 310130
            Orig. end                         :: 320358
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            RHGC01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10228
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10228
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10228
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5228](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5228)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(415..429)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.34"
                     /tool="antismash"
     gene            complement(1797..4028)
                     /locus_tag="EJ355_RS08730"
     CDS             complement(1797..4028)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014157698.1"
                     /locus_tag="EJ355_RS08730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_125262836.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 5.2e-37, bitscore:
                     122.6, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPPPGGRRRHRPEPPRRGRRAAPKGRRPRTVRTPTVLQMEAVECG
                     AAALAMVLAHHGRHVPLEELRIACGVSRDGSRASNVLKAARGYGLTAKGMQMEPAALAE
                     VEAPAILFWEFNHYVVYDGAGRRLGRRGVYINDPDKGRRFVPAEDFDTSFTGVALVLEP
                     GPDFRPGGRRPGVMGALPARLRGTTGTMLAALLASLLLVAVGAVLPALSRTYIDLFLIG
                     HQTSLLGALFASMGAMVALTVVLTWLQQANLLRGRIISSTVGSARFLRHLLRLPVTFYA
                     QRSPADLVQRLASNDTVAETLARDLTAAGVDGVVVLLYAALLWTYDPQLTLVGVGIALL
                     NVVAMRVVIRLRATGTQKLRADSAKLTNTSYTGLQLIETMKATGGEDGYFRRWAGQHAT
                     TLEEQQRLGVPSAWLGVVAPTLATLNSALILWLGGLRAVEGHLSIGLLVAFQALVTRFT
                     APITRLNGVAGRIQDFAADVARLKDVENFPVDALYARPEPAASTRRLTGHVTLDAVTFG
                     YSPLDKPLLTGFSLSVGPGQQVALVGGSGSGKSTVSRLISGLYSPWEGTIRIDGQRLED
                     IPRGALCASVSFVDQDVFLFEGTVRDNVALWDPSVPDDAVVAALKDACLYDVVARRPGG
                     IHGRVEQDGRNFSGGQRQRLEIARALVRRPSVLVLDEVTSALDAETEQGIIDNLRRRGC
                     ACVVIAHRLSTVRDSDEIVVLDHGQVVERGRHEHLVAAGGPYAHLVKEH"
     gene            complement(4082..4885)
                     /locus_tag="EJ355_RS08735"
     CDS             complement(4082..4885)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="EJ355_RS08735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_125262414.1"
                     /transl_table=11
                     /translation="MQFRQQALSKLQSPEELDVPVRYARPQGLLVLAVTLVVMAAAGVW
                     AVTGSVPSTLRSPGILTHGQGSYVLQSPVTGQVTRVLAKEGKRLAAGAPVLEVRTEQGD
                     KAVRAIAAGRVTTLAARIGTVVTTGSDVATLERVAGTDDPLLAMLYVPADSAALPVGTS
                     VDLAVQSAPTAEYGKLRGRVQAVGRAAQSRQKITGFLGDKELAGQFTQDGPPVAVVVRL
                     EKSTRTKSGYAWSTADGPPFPLDSMTPATGTFHLAEQRPIDWLLP"
     misc_feature    4983..4997
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.68"
                     /tool="antismash"
     gene            complement(5001..5228)
                     /locus_tag="EJ355_RS08740"
     CDS             complement(5001..5228)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014157700.1"
                     /locus_tag="EJ355_RS08740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_031192350.1"
                     /sec_met_domain="strepbact (E-value: 2.4e-33, bitscore:
                     110.5, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNVPQVETREISDADLDNVAGGLVSGVVGNVTGTVDSIAPVSGV
                     VGGVVGTVEGVTGVNTGAVTGLASGLTAGL"
     gene            5914..6594
                     /locus_tag="EJ355_RS08750"
     CDS             5914..6594
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1044: ABC
                     transporter, permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676573.1"
                     /locus_tag="EJ355_RS08750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter permease"
                     /protein_id="WP_031192349.1"
                     /transl_table=11
                     /translation="MTAPPDDCLARNEWICGDYLSTRREILTDAVLQHLELTALSVLIA
                     LAIALPLAVLARRWGLAAGPVLAVTTVLYTIPSLAMFSLLLPVYGLSETLVIAGLVLYS
                     LTLLVRNILAGLRAVPEDTRQAARGMGYGPIRLLLTVELPLALPAAMAGLRIATVSAVS
                     LATVGAIVGFGGLGNLIYSGMNTYFKAQVLTASVLCVVIAVLADLLLLGVQRLLTPWTR
                     AARA"
     gene            6591..7268
                     /locus_tag="EJ355_RS08755"
     CDS             6591..7268
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1044: ABC
                     transporter, permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016638956.1"
                     /locus_tag="EJ355_RS08755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter permease"
                     /protein_id="WP_087886884.1"
                     /transl_table=11
                     /translation="MNTLTDAWDWLADSAHWAGDDGIWHRLTQHLLLTVVCLLISCLIA
                     LPVALVLGHLGKGGALAVNISNVGRAVPTFAVLVLLLLTPLGKWGEGPTVVALVLFAVP
                     PLLTNAYVGMRGVDRSVVQAARGMGMTGRQMLWRVEAPLALPLIMSGVRIAAVQLVATA
                     TIAALAGGGGLGRIITAGFNLASTPQVVAGAVLVAVFALLVEGVFEAVERLAPGWARKT
                     GKG"
     gene            7319..8242
                     /locus_tag="EJ355_RS08760"
     CDS             7319..8242
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613031.1"
                     /locus_tag="EJ355_RS08760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_125262837.1"
                     /transl_table=11
                     /translation="MLLAAAALLASGCDSGPSLETQDKVTAPPGDSKHLVVGSAGFTES
                     DLLAQMYALLLDKAGYDTEILSVANRELYEPALESGQIDVVPEYAATFADWLNAKVNGA
                     DAKPVGSPDLDATMKALRKLAAPRGLTVLDPGRAVDQNAFAVTAAYARKHHLKSLTDLG
                     KSGLKVRLAAGDECVQRPYCEPGLKKVYGIDITGVDPKGVGTTQAKKAVQSGQDQMVLT
                     TTTDATLDEFGLVLLTDDKNLQNADYIVPVVNRSRAGSKGVSGALARLNTVLTTKDLAD
                     LNRQVDSWRRLPEDVAKKYLETKNLL"
     gene            complement(8563..9726)
                     /locus_tag="EJ355_RS08770"
     CDS             complement(8563..9726)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964372.1"
                     /locus_tag="EJ355_RS08770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="WP_125262416.1"
                     /transl_table=11
                     /translation="MIRFEQVTKRYPDGTTAVDDLSFEVAEGELVTLVGPSGCGKTTTM
                     MMVNRLIEPTSGRIYVNGEDIAAVDPVRLRRRIGYVIQQVGLFPHRTVLDNTATVPALV
                     GWKRAKARARAAELLDLVGLDPKTYGPRYPEQLSGGQRQRVGVARALAADPPVLLMDEP
                     FGAVDPVVRERLQNEFLNLQRTVRKTVLMVTHDIEEAVRLGDRVAVYGQGRIEQFDTPG
                     AVLGTPATPYVARFVGADRGLKRLSVTEIEVDDLEQPPVARLDESAAQAAARLRGDDAR
                     WAMVLDESGDLHGWVGVDEVGLAGDDAIVGDLAHRMNAWVPVGAPLKQAFGVMLQHDAG
                     WVAVVDGSRFVGVLTPAKLHEALRRSVDADAHGVGRGDVEFDSVADA"
ORIGIN
        1 cgcgcagggt ctggaacgcc tggttgttca gcttgttgcc gagctccacc agacgcctct
       61 gccagcgcag ttcccacagg cccagcagga ggaacacggc cgcgacgacg gcgagcatcg
      121 ccagggcggc cagggcgagg gggacgctgt agagcaggag caggaccacg ttcatcgcgc
      181 cgatggagcc tgcctgcagg gcgaccgggc cgatgcccga gaggacccgg cggatcgcgc
      241 tgacgcccat cgcggcgctc gccagctccc ccgtggagcg ggcggcgaag aagcgcgtgg
      301 gcagcctcaa gaggcggtcc cagaccgccg gttggagagc gctctccatg cggccctcca
      361 gccgcaggac ggtgaggttc tggagcagca tgaacgccgc cgagaccaag ctggtgatca
      421 tgacggccag ggccacctgg acgatgaggc tcttctcggc gttcggcacg aagacaccca
      481 gcacctggcc cgtggcgatg ggcaccagag cgccgaccac gaccgtcacc agcgacgcga
      541 gcgcgaggtt gcgcacgtcg ccaccgctgc cccgcagact gaagcgggcc aggcgccacg
      601 gggtcaacgc ccggtcgggc agcggacggt agaacatgac cgcgcgcggt tcgaactcgt
      661 cggcgttggc ggggccgacg cgggtgcgcc gcccggacgt ggggtgcacc gactcgtagc
      721 cgccgcgccg ccacagcagc gcgaccggcg cgccgctcgc ggcacggtgt cccaccagcg
      781 ggcccgcgtt ctcgcgccac cagcggccgt cgagccggac ggcacgggta cggatccgcg
      841 cggccaccgc gatctgctcc accgggtcga tccggtcgct caccgcaccg cccgccgcgg
      901 cgtcggtcag tgcgatgccc gccgctccgg ccaccaggcg gcaggcggcg tacgtcgcgt
      961 cgtgaccgcc acgggtgtcc ccgggacgcg aacggcgccc gcccgagcgg ccgatggagg
     1021 cgatcagcgt cctgtcggcc tgctcgcgca cgtgctcgcc cgccttgata ccggcggccg
     1081 tacggtcctc gtggtcccgt tccaggcgct cgatccaccg gtcgagggtg gccagaaggc
     1141 ggtactgctg gttgaccatc cgctgccaca tcgccgcgtc gacgagcagg tcgcccgcgg
     1201 cctgctcgct gtaggcggcg ccgtaccgca cgctgcccgg cgcgaccggc atccagagga
     1261 tgtcgtcttc ggccacggcg ccgtcggggg cggcggtgct gtcggggacg gcctcgtcgc
     1321 tcaggccgcg gccgtccagc ggcgcctcga acaggacccg gtggccgcgt ccgacgccga
     1381 gcgcgaaggc gtgctccagg aggctcagcg ccgcgtcctg ggtgtcgtac cggctggggt
     1441 accactgctc gtacgaccag gtgtcgccgt agtccgggcg gtacagctcg cgcagcggga
     1501 tccggcgcag cccacagccg tgcagggggc ggccgacgag ggtgtgctgg gggccctcga
     1561 ccgggccgag gagcagcgtg cccggctcca ggcggccgag gtagtgccag gggccctggt
     1621 gcacggcgtc gacggcgaac aggtcgaggg cgccggccac gacgagccaa agcacctggg
     1681 ggccttccag gtgcacgctg cgcaggcccg tgcagtcgac cggcgtgccg agcgctccga
     1741 aggcgccgcc gacggtgtcg tcgccgtacg cgtgctgttc cgggacggag gtcacgtcag
     1801 tgctccttga ccaggtgggc gtaggggccg cccgcggcga cgaggtgctc gtggcgcccg
     1861 cgctccacga cctgcccgtg gtcgagcacg acgatctcgt cgctgtcgcg gacggtgctc
     1921 aggcggtggg cgatgacgac acaggcacag ccgcgacggc gcagattgtc gatgatgccc
     1981 tgttcggtct cggcgtccag ggcgctggtc acctcgtcga ggacgaggac gctcgggcgg
     2041 cggaccaggg cccgcgcgat ctccaggcgc tgccgctgcc cgccggagaa gttccgcccg
     2101 tcctgctcga cccggccgtg gatgccgccg ggcctgcggg ccacgacgtc gtacaggcag
     2161 gcgtccttga gggcggcgac gacggcgtcg tccgggacgg aagggtccca cagcgcgaca
     2221 ttgtcgcgga cggtgccctc gaagaggaag acgtcctggt cgacgaacga gacggacgcg
     2281 cacagcgcgc cgcgcgggat gtcctccagg cgctgtccgt cgatgcggat cgtgccctcc
     2341 cacgggctgt agagccccga tatcagccgg gagacggtcg acttgccgct gccggagccg
     2401 ccgacgagcg cgacctgctg ccccggcccg accgacagcg agaagccggt caggagcggc
     2461 ttgtcgaggg ggctgtagcc gaaggtgacg gcgtcgaggg tgacgtggcc cgtgaggcgg
     2521 cgcgtgctcg cggccggttc ggggcgcgcg tagagcgcgt cgacggggaa gttctcgacg
     2581 tccttgaggc gggcgacgtc ggcggcgaag tcctggatgc ggcccgcgac gccgttgagg
     2641 cgggtgatcg gcgcggtgaa gcgggtcacc agggcctgga aggcgacgag caggccgatc
     2701 gacaggtgcc cctcgaccgc gcgcaggccg ccgagccaca ggatcagcgc gctgttgagg
     2761 gtcgccaggg tcggcgcgac gacaccgagc caggcgctcg gcaccccgag gcgctgctgc
     2821 tcctcgaggg tggtggcgtg ctggcccgcc cagcgccgga agtagccgtc ctcgccgccg
     2881 gtggccttca tggtctcgat caactgcaga ccggtgtacg aggtgttggt gagcttggcg
     2941 ctgtcggcgc gcagcttctg ggtgccggtg gcacgcagcc ggatgacgac gcgcatcgcc
     3001 acgacgttca gcagggcgat gccgacgccg acgagggtga gctgcggatc gtaggtccac
     3061 agcagggccg cgtagaggag gacgacgacg ccgtcgaccc cggcggccgt gaggtcgcgg
     3121 gcgagggtct cggcgaccgt gtcgttggac gccaggcgct gcacgaggtc ggcggggctg
     3181 cgctgggcgt agaacgtgac gggcaggcgc agcagatggc gcaggaagcg ggcgctgccg
     3241 acggtggagg agatgatgcg gccgcgcagc agattcgcct gttgcagcca ggtcagcacc
     3301 acggtcagcg cgaccatcgc gcccatcgac gcgaacagcg cgccgagcaa cgacgtctga
     3361 tgccctatca ggaacaggtc gatgtacgtc cggctgagcg cgggcagcac ggcgccgacc
     3421 gcgacgagca gcaggctggc caggagcgcg gcgagcatcg tgcccgtggt gccgcgcagc
     3481 cgtgccggga gggcccccat gacgccgggc ctgcggcctc ccggacggaa gtcaggaccc
     3541 ggttcgagca ccagagccac tccggtgaag ctggtgtcga agtcctccgc gggcacgaag
     3601 cggcggccct tgtccgggtc gttgatgtac acgccccggc ggccgaggcg gcgccccgcg
     3661 ccgtcgtaca cgacgtagtg gttgaactcc cagaagagga tcgcgggcgc ctcgacctcg
     3721 gccagcgcgg cgggctccat ctgcatgccc ttggccgtca ggccgtaacc gcgcgccgcc
     3781 ttcaggacgt tgctcgcccg ggagccgtcg cgggagacgc cgcacgcgat gcgcagctcc
     3841 tccaggggga cgtgccgtcc gtggtgggcg aggaccatgg cgagggcggc ggcgccgcac
     3901 tccacggcct ccatctgcag gacggtgggc gtgcggacgg tccgtggccg cctgcccttc
     3961 ggcgccgcac gccggccgcg ccgcgggggt tcggggcggt gcctgcgacg gccgccgggg
     4021 ggcggcagtg gctgctggtg cgcgggggat gcgggggatg cgggggaggg gagcggtgcg
     4081 gtcacggaag cagccaatcg atcgggcgct gctcggcgag gtggaaggtg ccggtggccg
     4141 gggtcatgga gtcgagcggg aagggcggcc cgtcagcggt cgaccacgcg tagccggact
     4201 tggtgcgcgt cgacttctca aggcgtacga ccacggccac gggcggcccg tcctgggtga
     4261 actgcccggc cagttccttg tcgccgagga agccggtgat cttctgcctg ctctgcgcgg
     4321 cccgcccgac ggcctgcacc cggccgcgca gcttgccgta ctcggccgtg ggcgcggact
     4381 ggacggcgag atccaccgac gtgccgacgg ggagggcggc gctgtcggcc ggtacgtaga
     4441 gcatcgcgag gagcgggtcg tcggtgcccg ccacgcgctc cagggtcgcg acgtcggagc
     4501 cggtggtgac gacggtgccg atcctggcgg cgagcgtggt cacgcggccg gcggcgatgg
     4561 cgcggacggc cttgtcgccc tgttccgtac ggacttcgag tacgggagcg cccgcggcga
     4621 gccgcttgcc ctccttggcg agcacgcggg tgacctggcc ggtgacgggg ctctgcagga
     4681 cgtagctgcc ctggccgtgc gtgaggatcc cgggggaccg cagcgtcgag gggaccgacc
     4741 cggtcacggc ccagacgccc gcggcggcca tgacgacgag ggtgacggcg aggacgagca
     4801 ggccctgggg gcgggcgtag cgcaccggta cgtcgagttc ctcgggcgac tgcagcttgg
     4861 acagggcctg ttggcggaac tgcacggaac gcttccctta ctgtgcctgg tcggtggcgt
     4921 cacggtgcct ggggcgtccg tgagccccgg aacctggggc tccggggctc acgtgtcacg
     4981 ccgtctcagt gagcgaaggc tcagagaccg gcggtcaggc cggaggccag gcccgtgacg
     5041 gcgccggtgt tcacaccggt gacaccctcg accgtgccga cgacgccgcc gacgacgccc
     5101 gagaccgggg cgatggagtc aacggtgccg gtgacgttgc ccacgacgcc ggagacgagg
     5161 ccaccggcga cgttgtcgag gtcggcgtcg gaaatctcgc gggtctcgac ctgggggacg
     5221 ttgttcatga tgtgagctgc ccttctcatg agtggtgcac aggtgtttcc aaggccgcgg
     5281 aacctgggga ttcgaattcc gcgacggcca ggatcaaagc atgttcgcag cgcgtacatc
     5341 caactggtga agtggcggtc agctgcggct gtgacgttga atcaccgctg cgtgcaggct
     5401 gtttcaccgc gtggtggcag ctccttcaca agcttcacag aagaaaattc ggtacccgga
     5461 atgactcctt ccaacaaatc ggacacggtc ccgcggaaaa gcgttgcctg tggtcgatgc
     5521 accgaaattc gagaggagcg cggattgaat ggcgtgacga aggtgcgcat tcgatgtgca
     5581 ggttcactga aggcacccgc aatcggggtc gaaggggacg ccgggtcgcc cgagcggggc
     5641 tgtcggccgg tccggctacc ggccccagtc cgggcggtcg gcgagcccgg cggcgacgaa
     5701 cgccgccact ggtgctccac ccggtgcggc gaccacgccc gcgccgcccg ggcccacgcc
     5761 cgccgcacag gttgacggca ccgcgggccc acgcccgccg cacggactga cggcaccccg
     5821 cgcccacacc ggctgcaccg gctgacggcg caccccaccc cgtacggtcc agcaccaggt
     5881 gccgaccccc ctgtggcccg ctggaatgga cgggtgaccg cgcctccgga cgactgcctc
     5941 gcgcgcaacg agtggatctg cggcgactat ctgagcaccc gccgcgagat cctcaccgac
     6001 gccgtgctgc aacacctgga gctgaccgcg ctctccgtcc tgatcgcgct cgccatcgcc
     6061 ctgcccctgg ccgttctcgc ccgccgctgg ggcctggccg ccggacccgt cctcgccgtg
     6121 accaccgtcc tctacacgat cccgtccctc gcgatgttct cgctgctgct cccggtgtac
     6181 ggactgtcgg agacgctcgt catcgcgggc ctggtcctgt actccctgac gctgctcgtc
     6241 cgcaacatcc tcgcgggcct gcgcgccgtc cccgaggaca cccggcaggc cgcccgcggc
     6301 atgggctacg gcccgatccg cctgcttctg accgtcgaac tgccgctcgc cctgcccgcc
     6361 gccatggcgg gcctgcgcat cgccaccgtc tcggcggtct ccctcgccac cgtcggcgcg
     6421 atcgtcggct tcggcggact cggcaacctc atctactccg gcatgaacac ctacttcaag
     6481 gcgcaggtcc tcaccgcctc cgtgctctgc gtggtcatcg ccgtcctcgc cgacctgctg
     6541 ctgctcggcg tacagcgact cctcacgccc tggacgaggg cggcccgcgc atgaacaccc
     6601 tcaccgacgc ctgggactgg ctcgccgact ccgcgcactg ggcgggcgac gacggcatct
     6661 ggcaccggct cacccagcac ctcctcctca ccgtcgtctg cctcctgatc agctgcctca
     6721 tcgcgctgcc cgtcgccctg gtcctcggcc acctcggcaa gggcggcgcg ctcgccgtga
     6781 acatctccaa cgtcgggcgc gccgtgccga cgttcgcggt cctcgtgctg ctcctgctca
     6841 cgccgctcgg caagtggggc gagggcccca ccgtcgtcgc cctggtcctg ttcgccgtgc
     6901 cgccgctgct caccaacgcc tacgtcggca tgcgcggcgt cgaccgcagc gtcgtccagg
     6961 ccgcgcgcgg aatgggcatg acggggcggc agatgctctg gcgggtcgag gcgccgctcg
     7021 cgctgcccct gatcatgagc ggcgtccgga tcgccgccgt ccagctcgtc gccaccgcga
     7081 ccatcgccgc gctcgcgggc ggcggcggcc tcggccgcat catcacggcg ggcttcaacc
     7141 tggccagcac gccccaagtg gtggcgggcg cggtgctcgt cgccgtgttc gccctgctgg
     7201 tcgagggcgt cttcgaggcc gtcgagcggc tcgcgcccgg gtgggcgcgc aagaccggga
     7261 aggggtgagt gacgtgacac gcaccgttcg ccgacgcaac cggcgcaccc ggtgcggcgt
     7321 gctgctcgcg gccgccgcac tgctcgcctc cggctgcgac tccggtccct ccctggaaac
     7381 ccaggacaag gtcaccgcgc cgcccggcga cagcaagcac ctcgtcgtcg gctccgccgg
     7441 attcacggag agcgacctgc tcgcgcagat gtacgcgctc ctcctggaca aggccggcta
     7501 cgacaccgag atcctgtccg tcgccaaccg cgaactctac gaacccgccc tggagtccgg
     7561 ccagatcgac gtcgtgcccg agtacgcggc caccttcgcc gactggctga acgcgaaggt
     7621 caacggagcc gacgcgaagc ccgtcggctc acccgacctc gacgccacca tgaaggccct
     7681 gcgcaagctc gccgcgcccc gcggcctcac cgtcctcgac cccggcaggg ccgtggacca
     7741 gaacgcgttc gccgtcaccg ccgcctacgc ccgcaagcac cacctgaagt ccctcaccga
     7801 cctcggcaag tccggcctga aggtccgcct ggccgcgggc gacgaatgcg tccagcgccc
     7861 gtactgcgaa cccggcctga agaaggtcta cggcatcgac atcaccggcg tcgaccccaa
     7921 gggcgtcggc accacccagg ccaagaaggc cgtccagagc ggccaggacc agatggtcct
     7981 caccaccacg acggacgcca ccctcgacga gttcggcctg gtcctcctca ccgacgacaa
     8041 gaacctccag aacgccgact acatcgtccc cgtcgtcaac cgctcccgcg cgggcagcaa
     8101 gggcgtctcc ggcgccctgg cccgcctcaa cacggtcctg acgacgaagg acctggcgga
     8161 cctcaaccgg caggtggaca gctggcgcag gctgccggag gacgtggcga agaagtacct
     8221 ggagaccaag aacttgctgt agcgccagcg attacgcccg cctgccgcct gccgcctgcc
     8281 gcctgccgcc tgccgcctgc cgcctgccgc ctgccgcctg ccgcctgccg cctgccgcct
     8341 gccgcccacc gcccgccgcc tgccgcccac cgctccacct cccccggacg gggctctctt
     8401 ctccgccgca acggcgaaat cccccacaag catcccggcg gcgccaaaca ccccccagcc
     8461 atggcgcagc caaaatccac agaaggcccc agacaaaccc cagccatggc gcagccaaga
     8521 gccccccgca ggggaccccg cccaccccgc gccggaaagg cgctacgcgt ctgcgaccga
     8581 gtcgaactcc acgtcccccc gccccacccc atgggcatcc gcgtccacag accgccgcag
     8641 cgcctcatgc agcttcgccg gggtgaggac gccgacgaag cgggagccgt ccacgacggc
     8701 gacccagccc gcgtcgtgct ggagcatcac gccgaaggcc tgcttcaggg gtgcgccgac
     8761 gggcacccag gcgttcatgc ggtgggcgag gtcgccgacg atggcgtcgt ccccggcgag
     8821 gccgacctcg tcgacgccga cccagccgtg caggtcgccg ctctcgtcga ggaccatcgc
     8881 ccagcgggcg tcgtccccgc gcagccgggc cgcggcctgc gcggcggact cgtcgaggcg
     8941 ggcgacgggc ggctgttcga ggtcgtcgac ctcgatctcg gtgacggaga gccgcttgag
     9001 cccgcggtcg gcgccgacga acctggccac gtacggcgtc gcgggcgtgc cgaggacggc
     9061 gcccggtgtg tcgaactgct cgatgcgccc ctgcccgtac acggcgaccc ggtcgccgag
     9121 gcggacggcc tcctcgatgt cgtgggtgac catcaggacg gtcttgcgca cggtccgctg
     9181 gaggttcagg aactcgttct ggagccgttc gcgcacgacg gggtcgacgg cgccgaacgg
     9241 ctcgtccatc aggagcaccg gcgggtcggc ggcgagggcg cgggccacgc cgacgcgctg
     9301 gcgctgtcca ccggagagct gttcggggta gcgcggcccg taggtcttcg ggtcgagccc
     9361 gacgaggtcg aggagttcgg cggcgcgggc ccgcgccttg gcccgcttcc agccgacgag
     9421 ggcgggcacg gtcgcggtgt tgtcgaggac ggtgcggtgc gggaagaggc cgacctgctg
     9481 gatgacgtag ccgatgcggc gccgcaggcg tacggggtcg acggcggcga tgtcctcgcc
     9541 gttgacgtag atgcggccgg aggtcggctc gatgagccgg ttcaccatca tcatcgtggt
     9601 ggtcttgccg cagccggacg ggccgacgag cgtgaccagc tctccctcgg cgacctcgaa
     9661 ggagaggtcg tccacggccg tcgtgccgtc cgggtagcgc ttggtcacct gctcgaaccg
     9721 gatcattccc tcacgctaac cgtgcgtgtt ccaccccgca cacgctcggt gaccgctcag
     9781 ggggactcct cggggctccc ggtcaccagg accacctcca gggtgcgcgg cccgtgcaca
     9841 ccctcgacgc ggtcgagttc gatgtcgctg gtcgcggaag gaccggagat ccaggtgagg
     9901 gggcgcccgg ggtcgagccg ctccagggcc tggggcacgg aggagacgac ctggtcgggg
     9961 acgcggacga cgcagatgtg gtggtcgggg acgagggtga tgcggcggcg gccctggtcg
    10021 ggggagctgt cgaggacgag ggtgccggtc tcggcgatcg cgatcgcaca gccggtgacg
    10081 acgctgtcgg cccggtccag ctccgcggtg gtgctcgcgg cccggtcatg gatgcggacc
    10141 gggtcggcgg ccgcgagcca gcccggcggc agcgcgggcg gcacgaggac gttccgggcg
    10201 ccgcgatacg cgagcagccg catcagca
//
