LOCUS       NZ_CP034118            10852 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Staphylospora marina strain SCSIO 07575 chromosome, complete genome.
ACCESSION   NZ_CP034118
VERSION     NZ_CP034118.1
KEYWORDS    RefSeq.
SOURCE      Staphylospora marina
  ORGANISM  Staphylospora marina
            Bacteria; Bacillota; Bacilli; Bacillales; Thermoactinomycetaceae;
            Staphylospora.
REFERENCE   1  (bases 1 to 2706111)
  AUTHORS   Wang,K.X. and Tian,X.P.
  TITLE     Staphylospora marinus gen. nov., sp. nov., a novel member of the
            family Thermoactinomycetaceae, isolated from a deep-sea hydrothermal
            vent sediment in Pacific Ocean
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2706111)
  AUTHORS   Wang,K.X. and Tian,X.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2018) CAS Key Laboratory of Tropical Marine
            Bio-Resources and Ecology, South China Sea Institute of Oceanology,
            No. 164 West Xin Gang Road, Guangzhou, Guangdong 510301, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: FALCON v. 0.3.0; Celera Assembler v. 8.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1382.0x
            Sequencing Technology             :: PacBio Sequel; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003856495.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 03:57:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,772
            CDSs (total)                      :: 2,679
            Genes (coding)                    :: 2,660
            CDSs (with protein)               :: 2,660
            Genes (RNA)                       :: 93
            rRNAs                             :: 7, 6, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 4 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 0 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:09:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2099297
            Orig. end                         :: 2110149
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP034118.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10852
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="8"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10852
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10852
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5852](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5852)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            888..1232
                     /locus_tag="EG886_RS10380"
     CDS             888..1232
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013859576.1"
                     /locus_tag="EG886_RS10380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YolD-like family protein"
                     /protein_id="WP_124728076.1"
                     /transl_table=11
                     /translation="MSGGEGLDRKNMLWEGSRMFLPEHRQALLRQRAEAERVEPPELDE
                     DRLEEINRLVVEALHADFPLVVRWVENGRLTEFCGFIQKVDPHARQLKLADGRTVRTVP
                     FDRLCDAHRP"
     gene            complement(1385..2632)
                     /locus_tag="EG886_RS10385"
     CDS             complement(1385..2632)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1184: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /locus_tag="EG886_RS10385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_164491792.1"
                     /transl_table=11
                     /translation="MSPASVPGTSGEIAPRPEPGKILLLALSFTAFLVGMEAIVTVPLI
                     PGMSASTGMPLKSGGMLVAAYAISYALFAPVAGMISDRLGRKSVLAAGMGLFTVATALV
                     GFAGSFSELLVFRVLTGIGAGMVQPSVFAVAADAWPEQMRGRVMGIVTGSLVAASVLGI
                     PLGGWLAELGSWRWTFWLLAALIPVAAAGLLTRMPAAAPGTAGRVPLSVMLRDAAADKG
                     VLASLGASALFFGGLQGMYSQSGAVYRLYFDLDVGAISLVLMVAGIGSVLGSLVGGRLA
                     DTRGKSRLLVLASLTVSVMILLFALVVDLAVSIWLVLVIQFVWALMYGVGHASLNARIA
                     ELRPEARGTVLSLNASAMYVGSASLSFLATWAFESVDLWLAGLLCAVANWGVGWLAARY
                     LRERNPEEQASSESVH"
     gene            complement(3152..4039)
                     /locus_tag="EG886_RS10390"
     CDS             complement(3152..4039)
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /locus_tag="EG886_RS10390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DMT family transporter"
                     /protein_id="WP_124728078.1"
                     /transl_table=11
                     /translation="MGIWNESKRMTAPEKTLWLFVGVTAVSFSAIFIKWSEAPAGVLGM
                     YRLFIAMAFMIPWVWRRKKEWGNLTGRDWGWLFLSGLFLGLHFWGWISSFRYTSVASSM
                     VLLSTGPLFAAWGARVVFRRNIGRMALFGMMLAVTGSALVAGADLQVGGTAWIGDLLSL
                     FGAFAVSVHMLIGERLREKLSTSIYSFSVFGVAGLFLAGMNAWQEMPLFTWPAREWGIF
                     FLLALVPTVFGHVLFNRLLRQVDAVTIQTAVLGEPVGAILLAFWLLGEPVTPVQILGCM
                     LTVGGIAWYLRRSS"
     gene            complement(4080..4259)
                     /locus_tag="EG886_RS10395"
     CDS             complement(4080..4259)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EG886_RS10395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_124728079.1"
                     /transl_table=11
                     /translation="MDKDPLYYELMVKLYFEREREGNRGTGRNAPPSGKCGPWWRAKRT
                     MKRAGQWLRITRRW"
     misc_feature    4440..4455
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 21.65"
                     /tool="antismash"
     misc_feature    4442..4456
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.7"
                     /tool="antismash"
     misc_feature    complement(4443..4458)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 21.45"
                     /tool="antismash"
     misc_feature    4468..4486
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 24.17"
                     /tool="antismash"
     misc_feature    complement(4471..4486)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 20.93"
                     /tool="antismash"
     gene            4515..4979
                     /locus_tag="EG886_RS10400"
     CDS             4515..4979
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EG886_RS10400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_124728080.1"
                     /transl_table=11
                     /translation="MNREIDGLIDIHTILSHERKLGGAVEADFIRLNSGEMFQSPVITR
                     IDVTGGNVYTVSFVDESGRKFIVHVNEIAVIGSPGHKRIHELNNRYGKQMLTARKLAYL
                     KRLCEVNAGSSDPFFVKEVIVILDDIGMDAVNEVLHPELLPVIRKTLSAA"
     misc_feature    4528..4543
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.74"
                     /tool="antismash"
     gene            complement(5001..5852)
                     /locus_tag="EG886_RS10405"
     CDS             complement(5001..5852)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003332270.1"
                     /locus_tag="EG886_RS10405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_124728081.1"
                     /sec_met_domain="Linocin_M18 (E-value: 2.2e-81, bitscore:
                     270.0, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKLTRFPSAPLREEEWEALEKTVVETAARQLVGRRFIDLYGPLG
                     EGIQTVVNDFYENPERGMLSLHGDEPDLSVPNGRVNLTVPMLYKDFVLYRRDIDQSRSL
                     DIPVDFSPAANAAQQCALLEDELIFNGSEKFGLPGLMNVKGRLTHIRSDWMKSGNAFAD
                     VVEARNKLMAMGHTGPYALVLSPTLYAMLHRVHEGTRVLEVEHVRELMTDGIYQSPVIR
                     GDAGVIVSTGRQNLDLAVSEDMDVVFLDTDNMNYLFRVYETLVLRIKRPGAICTLESPT
                     GT"
     gene            complement(5868..6455)
                     /locus_tag="EG886_RS10410"
     CDS             complement(5868..6455)
                     /GO_function="GO:0140315 - iron ion sequestering activity
                     [Evidence IEA]"
                     /GO_process="GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04536.1"
                     /locus_tag="EG886_RS10410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="IMEF encapsulin system ferritin-like cargo
                     protein"
                     /protein_id="WP_124728082.1"
                     /transl_table=11
                     /translation="MTERFAELAGILNRTREAVTEFMNMLDPVIREATDDYERLYYHHI
                     YEEEEHRLDRLRDLLPKVEAMAERSGDRDPDDPEWVYLLQDLGLEKFGLHNFLEHIDLA
                     LHHFQDEERTGELRRMREMTHDDYGAIKDILEELNERYGEAVSGRGSVPTDEKEDVADD
                     LKVPLHSDPAAPDPKPDPASLRKRWTVGSLKH"
     misc_feature    6512..6525
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 17.16"
                     /tool="antismash"
     gene            complement(6749..7699)
                     /locus_tag="EG886_RS10415"
     CDS             complement(6749..7699)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013524605.1"
                     /locus_tag="EG886_RS10415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nucleoside recognition domain-containing protein"
                     /protein_id="WP_124728083.1"
                     /transl_table=11
                     /translation="MSRINWRKGFESGWKTALELVKVVFPVALAVHLAKQTPLLDLLAD
                     LLSPVMGWFGLGGEAAIPLVLGNVLNLYAALGAIQALDLTVKQVFVLAVMLSFSHNLFI
                     ETALCRRVGVSALLIASVRVGLALVSALLIHWLVPFGDRPAGFRLVSGEPAGENTWMEV
                     AGSAILASLEGVIQLVVIVIPLMLMIQVLKDLGVMERTARLLAPAMKLLGIAPTGAVTL
                     ASGLFAGLFFGAGLIIQEAREQNLSRRDIALIMVFLAACHAVVEDTLVFVPLGIPVLPL
                     LLIRLAAAILITLVIARLWKVPAVQSYATAGDGKP"
     misc_feature    complement(7686..7699)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.57"
                     /tool="antismash"
     gene            complement(7788..9194)
                     /gene="rlmD"
                     /locus_tag="EG886_RS10420"
     CDS             complement(7788..9194)
                     /EC_number="2.1.1.190"
                     /GO_function="GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="rlmD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013877501.1"
                     /locus_tag="EG886_RS10420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="WP_124728084.1"
                     /transl_table=11
                     /translation="MSGYRMRLKKGEVIELPVRRIGINGEGVGYFRKQVVFVEGAVPGE
                     TVTARVTEVDKRHARARIIRVNKKSAYRTKPECPVYRECGACQLMHIDRRMQKRLKREL
                     VQEAFARYTPFDEVSVDPVVVMDEPRGYRNKAQLPVRKVGGRVVTGLYAAGSRRLVETS
                     GCLVQHPVLDRVLSGVAGILGELNIPVYDERTHRGSVRHLAARVSFAFGDVQLMIVSRT
                     ETLPKEDELVRLVHRRLPEVKSIVLNHNPDRTPLVLGEKSRVLFGPKKLRERLGDLEFR
                     LSARSFFQLNPVQTVRLYEEVRKAARLTGTETVVDAYCGAGTIGLWLARDAARVIGMDT
                     VPDAIEDARENADINGIRHAEFHVGRAEELLPEWLKDGLRPDVVVTDPPRTGLGEALIR
                     ALIEVKPPRLVYVSCNPSTLAKDVAKLAAGGYVPEKVVPVDMFPETAHVEAVCVLTVSG
                     GGPGGERAGQ"
     gene            complement(9575..10240)
                     /locus_tag="EG886_RS10425"
     CDS             complement(9575..10240)
                     /GO_function="GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009432 - SOS response [Evidence IEA]"
                     /GO_process="GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /locus_tag="EG886_RS10425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SOS response-associated peptidase"
                     /protein_id="WP_124728085.1"
                     /transl_table=11
                     /translation="MCGRFVLDADKRELMERFLLEALPEEVPPRYNIAPTQPVLTVMRD
                     EGTMKADWFRWGLVPAWSDSPHPRFRMINARAETLAEKPSWRRLLKRRRCLIPATGYYE
                     WKAVSGKKQPYLIRLPGGKLFSFAGLWDEWERDGQVIRSCTIITTEANERARDVHDRMP
                     VILTPQTETMWLDMQDTDPGVYRELLMSVPDPDLEMYPVSTRVNSPKAEGEHLIHPVE"
     misc_feature    complement(10387..10399)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 19.4"
                     /tool="antismash"
ORIGIN
        1 agcgagaagc tgttcggaga tccctggacg atcgcccgca aaatgcagga agccatccgc
       61 atccgcctcg gagtccgggc ccgcatcggc atgggcccca acaaagtgct gagcaagatg
      121 gcttgcgatc atttcgccaa aaaaaacgaa agcggcatct tccgtctgga tcattccaat
      181 ctggagagtc acctgtggcc cctgccggtg gagaagatgt tcggagtggg cttgcgcatg
      241 aagaaacacc tgaaccgcac gggcatccgc acgatcgggg atttggcccg ttttccgctg
      301 caccggctga aaaaacgctg gggcatcatg gggcacgtgc tgtggatgac cgccaacggt
      361 caggacgcct cgccggtcag tcgggaaacg tttgaccggc aaaaagcgat cggtcaccac
      421 atgacgttgc cccgggatta tcacacggcg gaggagatcc gggtggtgct gctggaattg
      481 tgcgaggaag tgtgccggag agcccgccgg cacggactga tgggccggac ggttcacgtg
      541 gggtgccgcg gagccgattt ttccctgccg acgggattcc accgtcaagc gggaatgccg
      601 gaagccaccc atgacgcttc caccttgttc cgccacgcct ggcgggtgtt tgaaaagttt
      661 tgggacggaa aaccggttcg ctcgctgggc gtgacactgg cgaacctgca ggatgaccgg
      721 acggttcagc tcagcctgtt cgaagatcgg gacagacaga tgcggctcgg agcggccatg
      781 gacgagatca gaagccgctt cggtccgacg gcgattctcc gggcgtcttc cctggtaccg
      841 gccggtcagg ccagggagcg ggccgtgaag atcggagggc atgcccggtg agcggcggcg
      901 aaggactgga tcgcaagaac atgttgtggg aggggagccg gatgttcctg cccgagcacc
      961 gacaggcgct gctccggcaa agagcggagg cggagcgggt ggaaccgccg gagctggatg
     1021 aagatcggct ggaagagatc aaccggctgg tcgttgaagc gctgcatgcc gactttccgc
     1081 tggtggtccg ctgggtggaa aacggccgac tgacggagtt ctgcggtttc atccaaaagg
     1141 tggatcccca tgcccggcaa ctgaaactgg ccgacggccg caccgtgcgc acggttccgt
     1201 ttgatcggtt gtgcgacgcg cacagaccgt gaaggttgaa aaagaaagaa gccctgccga
     1261 tgaggcggag ctttcggccc gtcgggcaac aaaaaaccgc cggcgcaccc ggcggaacca
     1321 ttcttccccc tgacccggac accggagaac gtcatccgtg tcccggagag gtctgttgca
     1381 tcggtcagtg aacggattcg gaagacgcct gctcttccgg attccgttcc ctgagataac
     1441 gggcggccag ccagccgact ccccagttgg cgacggcaca caacagaccc gccaaccaca
     1501 ggtccacgct ttcaaacgcc caagtcgcca agaaggacag cgacgcggaa ccgacgtaca
     1561 tcgcggaagc gttgagggac agcaccgtgc cccgcgcctc cggacgcaac tcggcgatcc
     1621 gggcgttcag cgacgcatgc cccaccccgt acatcagcgc ccagacaaat tgaatgacca
     1681 acaccaacca gatggaaacc gcaaggtcga ccaccagggc aaagagcaat atcatcaccg
     1741 agacggtcag ggatgccagc accagcaacc gactcttgcc acgggtgtcc gccaggcgac
     1801 cgcccaccaa acttcccagc acactgccga tgccggcgac catgagcacc aggctgatcg
     1861 ctccgacatc gagatcgaaa tagagacgat acaccgcgcc ggactggctg tacattccct
     1921 gcagcccgcc gaaaaacagg gcggaagctc ccagcgaagc aagcactccc ttgtccgcgg
     1981 cggcgtcgcg aagcatgacc gacagcggaa ccctcccggc ggttcccggc gcagcagccg
     2041 gcatgcgcgt cagcagtccg gcggcggcca cgggaatcaa cgcggccagc agccagaagg
     2101 tccaacgcca gctgccgagc tccgccaacc atcctcccag cggaatgccc agaaccgaag
     2161 cagcgaccag cgatcccgta acgatgccca tcacccggcc gcgcatctgc tccggccatg
     2221 catccgcggc gacggcaaac accgagggtt gcaccatccc cgctccgatc ccggtcaaaa
     2281 cgcgaaacac caggagttcg gagaacgaac cggcaaatcc gaccaacgcg gtggccaccg
     2341 taaacaaccc cattcccgcg gcgagcacgc ttttccggcc caaacggtcc gaaatcattc
     2401 cggccacggg agcaaacaag gcgtacgaga tggcatacgc ggccacgagc atgcccccgg
     2461 acttcagcgg catgccggtg gaagccgaca ttccgggaat gagaggaacc gtgacaatgg
     2521 cttccattcc caccagaaac gcggtgaacg acagagccag cagcaaaatc tttccgggct
     2581 ccggacgggg agcgatctcc ccggatgtgc ccggcacgga agcgggactc atgcgggatt
     2641 cctccttcat gacgaaatgg gtggagcctg tctttgcctt ttctcatgaa aggcgtgcac
     2701 tccaccggac atctccctct tttcctacgt atacaaaaat atccgtttcg ccatgaatcc
     2761 accgatttcc tttgataaat gatatgatct tgcatgagtc cggcgatttc tccctctccc
     2821 tggtcctgga gcgggccgga ctgatccgga tggactcgat aacggttgat ccctcccgcg
     2881 aaagcgatgt tcttcacaac gccgaaaccg ttcgctttcc ctgttcaagg gattcaggcg
     2941 cccttttggg ccgcatgtgg acgcaggatc ttgacagatc ccggcagttc cggattctga
     3001 cattctggag acacgagaaa gtgcatcacc atgaatcaca aagcgaaata cccgcatttg
     3061 tgacgcagcc ggagtccatg gggatcgaac accccgtcat cagccgtctg ttccttccgg
     3121 agccgaggtg gattgtcatc ccggactgag ctcatgacga ccggcgcaaa taccaagcga
     3181 ttccgccgac cgtcagcatg cagccgagaa tctgcaccgg ggtgaccggc tccccgagga
     3241 gccagaatgc cagcaaaatc gcgcccaccg gctcacccag aacggccgtt tgaatggtca
     3301 ccgcatccac ttggcgaagg aggcggttga acagcacatg cccgaacacg gtgggaacca
     3361 gagccaaaag aaagaaaatg ccccattccc gcgccggcca agtgaacaga ggcatctcct
     3421 gccaagcatt cattccggcg agaaacaaac cggccactcc gaacacggaa aaactgtaga
     3481 tggaagtgga caatttctcc cgcagccgct ccccgatcaa catgtggacg gagacggcaa
     3541 acgctccgaa cagggaaagc agatccccga tccaggccgt tcctcccacc tgcaggtccg
     3601 caccggccac caacgcggaa cccgtcaccg ccagcatcat cccgaaaagg gccattctgc
     3661 cgatgttccg gcggaacacc acccgggctc cccacgcggc aaagagcggt ccggtgctga
     3721 gcaacaccat ggaactggcc acactggtat aacgaaacga agagatccat ccccaaaaat
     3781 gaagccccag aaagagtccg gaaagaaaca gccatcccca gtcacggccc gtcagatttc
     3841 cccactcttt ctttcgccgc cagacccagg gaatcatgaa cgccatggcg atgaacaacc
     3901 ggtacatccc cagcaccccg gcgggtgcct ccgaccactt gatgaaaatg gcggaaaaag
     3961 agaccgccgt caccccgaca aacagccaca gggttttctc cggggcggtc atccgttttg
     4021 attcattcca aatccccatc ggtttctctc cttcagaaag caaacggcgg agaaggccgt
     4081 caccatctcc gcgtgattcg aagccattgc cccgcccgtt tcatcgtccg tttcgcccgc
     4141 caccacgggc cgcattttcc gctcggcgga gcattccggc cggtcccccg gttcccttcc
     4201 ctctcccgtt caaagtacaa tttcaccatc aattcatagt acaggggatc cttgtccaat
     4261 cccgtccacc tccggcgaaa aagacgggga aacatccctg tcgggtttcg acctttcgcg
     4321 gttgaaaatg ttcccggagc gtgcggacga accggcccgt catggcagac aggggtcgcg
     4381 ctctacgagt gacttcaaag gaatcattta cctttcacaa tccattgcca tcacttgacg
     4441 ataagaatca ttttcaatta gtattctacc gagaatgact ctcaaaggaa acaggttttc
     4501 cgggaggtag cggaatgaac agggaaattg acggtctgat cgacattcat accatcttgt
     4561 cccatgagcg gaaactggga ggagccgtgg aggccgactt catccggctg aattcggggg
     4621 aaatgtttca atcgccggtg atcacccgaa tcgatgtgac cggagggaac gtttacacgg
     4681 tcagtttcgt ggatgaaagc ggacgcaaat tcattgttca cgtcaacgaa atcgccgtga
     4741 tcggttcccc cggacacaaa cggatccatg aattgaacaa ccggtatggc aagcagatgc
     4801 tgaccgcccg gaaactggct tatctgaagc gtctgtgcga agtaaacgcg ggaagttccg
     4861 atcccttctt cgtgaaagaa gtgatcgtca ttctcgatga catcggcatg gacgcggtca
     4921 acgaagtgct gcatccggaa ctcctgccgg tgatccgcaa aacgttgagc gccgcgtgaa
     4981 acggatgttc gccggccgac tcatgttccc gtcggggatt ccagcgtaca gatcgcgccc
     5041 ggtcgtttga tccggagcac cagcgtttcg tacaccctga acagataatt catgttgtcc
     5101 gtatccagga acaccacatc catgtcttcg gagaccgcca gatcgaggtt ttgccgcccg
     5161 gtcgacacga tgacaccggc atccccgcga atgaccggag attggtagat cccgtccgtc
     5221 atcagctcac ggacatgctc cacttccagc acacgcgtgc cttcgtggac ccgatgaagc
     5281 atggcataaa gtgtcgggga caacacgagg gcatacggac cggtgtgtcc catggccatg
     5341 agtttgttgc gggcttccac cacatcggca aaagcattcc cggatttcat ccagtcgctc
     5401 cgaatgtggg tgagcctgcc tttcacgttc atcagacccg gcaagccgaa cttttccgat
     5461 ccgttgaaga tgagttcatc ctccaaaagc gcacattgct gggccgcgtt ggccgccggc
     5521 gaaaaatcga ccggaatgtc gagggaccgg ctttggtcaa tgtccctgcg gtacagcacg
     5581 aagtctttgt acagcatggg caccgtcaaa ttgacccgtc cgttcggaac ggacaaatcc
     5641 ggctcgtctc cgtgcagact caacattccc cgttccgggt tctcgtagaa atcgttcacc
     5701 acggtctgga ttccttcccc aaggggaccg tacaaatcga tgaatcttct tccgaccaac
     5761 tgtctggcag ccgtctccac caccgttttt tcaagcgcct cccattcttc ctcgcgaagc
     5821 ggtgcggacg gaaaacgtgt caacttgctc atcgcgttca gcctccctca gtgtttcaga
     5881 cttcccacgg tccagcgttt ccggagcgaa gcgggatccg gcttcggatc cggggctgcc
     5941 gggtcggaat gcagcggaac cttcagatca tccgccacgt cctctttttc gtccgtcgga
     6001 accgaacctc tcccggaaac cgcctcaccg taacgctcat tgagttcttc cagaatgtcc
     6061 ttgatggctc cgtaatcgtc atgggtcatc tctctcatgc gccgcaattc cccggtccgt
     6121 tcttcgtcct gaaaatgatg cagggccaga tcgatatgtt cgagaaaatt gtgcaatccg
     6181 aacttctcca agccaagatc ctgcagcagg tacacccatt ccggatcatc cggatcccgg
     6241 tctccggagc gctccgccat ggcttcgacc ttgggcagca aatcccgcaa gcgatcgagc
     6301 cggtgttctt cctcttcata gatgtggtga tagtacaaac gctcataatc gtcggttgcc
     6361 tcacgaatca ccggatccag catgttcatg aattcggtga ccgcctcccg ggtccggttc
     6421 agaatgccgg ccaattccgc gaaacgttcc gtcatgaacc tcccctcgct ttcacagcct
     6481 cttcctcccc aatatcaccc gaaaccatgc gatttaaaca gaaaaacccc gtccacgaag
     6541 gaacgggggg cgaaccggca acacgtgcgg aatgacgttt tcaaaaggtc actttcctgg
     6601 caagatatga tgacgaaggc tttcgaagtc agcggattca gcgatgaaga tctggtcacc
     6661 cgatgattcg ccacagtgga aaccccggga atttcccggg gttccggttg atcggatgga
     6721 ttcaaagatc cgcggaaagc cggaaccgtt acggtttgcc gtcccctgcg gtcgcgtacg
     6781 attgcacggc cgggactttc cacagtctgg cgatgaccag ggtgatcaga atcgcggccg
     6841 ccagccggat cagcagaagg ggaagcacgg gaattccgag cgggacaaag accagcgtat
     6901 cctccacgac ggcatgacag gcggccaaaa acaccatgat cagcgcaatg tcacggcggg
     6961 acaggttttg ctcgcgcgct tcctggatga tgagtcccgc cccgaaaaac aatccggcaa
     7021 acagtccgct ggccagcgtg acggctcccg tcggcgcaat gcccagcagt ttcatcgcgg
     7081 gagccaagag gcgggccgtc ctctccatca ctcccagatc tttgagtacc tggatcatca
     7141 gcatgagagg aatgacgatg acgaccaact gaatgactcc ttccaacgag gccaggatcg
     7201 ccgatcccgc cacttccatc cacgtgttct ctcccgccgg ctccccggaa accaaccgga
     7261 atccggccgg acgatctccg aacgggacca gccagtgaat gagcagcgcc gaaacgagag
     7321 ccaaaccgac gcgcaccgag gcgatcagca gggcggagac gcccacgcga cggcacaacg
     7381 ccgtttcaat gaacaaattg tgcgagaagc tgagcatcac cgccaacaca aacacttgtt
     7441 tcacggtcag atcgagcgcc tgaatcgctc ccagggccgc atacagattg agcacgtttc
     7501 cgagcaccaa cggaatggcc gcttctcccc cgagaccgaa ccaccccatc acgggtgaca
     7561 acaggtccgc cagcagatcg agcagcggcg tctgtttggc aagatggacg gcaagcgcca
     7621 cgggaaagac caccttgacg agctcgaggg ccgtcttcca tcccgactcg aagccttttc
     7681 tccagttgat ccgcgacatg gttctctcct ctctccgttt ctttcaacgt tccatccgct
     7741 tccccgtcca tccgacggac ctcttccggc agacgaccgg cgaagggtca ctgcccggcc
     7801 ctttccccac ccggtcctcc gccggacacc gtcaacacgc acaccgcttc cacatgggcc
     7861 gtctccggaa acatgtccac cggcaccact ttctccggaa catacccacc cgcggcaagc
     7921 ttggccacgt ccttggccag cgtggacgga ttgcaggaga cgtacaccag gcggggcggc
     7981 ttcacttcga tcagcgcgcg gatcaacgct tctccgagtc ccgtgcgcgg aggatcggtg
     8041 accaccacat ccggcctcaa tccgtccttc agccactccg gaagaagctc ctccgcccgg
     8101 ccgacgtgaa attcggcatg acggatgccg ttgatgtccg cattttcccg tgcgtcttcg
     8161 atcgcatcgg gaacggtgtc catgccgatc acccgggccg cgtcccgggc cagccacaag
     8221 ccgatcgtgc ccgctccgca gtaggcgtcc accaccgtct ccgttccggt gagacgggcg
     8281 gctttgcgta cctcctcgta cagacggacc gtctggacgg gattgagctg aaagaacgaa
     8341 cgggcagaca ggcggaactc cagatctccc aagcgctccc tgagtttttt cggcccgaaa
     8401 agaacccggc tcttttcgcc gagcaccagc ggggttctgt ccggattgtg attgagcacg
     8461 atgcttttca cttccggcag ccgacgatgc accaagcgta ccagttcgtc ttctttcggc
     8521 aatgtttcgg tgcgggagac gatcatcagc tgaacatccc cgaacgcaaa agagaccctc
     8581 gccgccaggt gcctcaccga tccgcgatgg gttctttcgt catacacggg aatattcagt
     8641 tctccaagga tccctgccac tccggaaaga acgcggtcca ggaccggatg ttgcacgaga
     8701 caaccgcttg tttccaccaa ccggcgggat ccggccgcat agagtccggt caccaccctg
     8761 ccgcccacct tccgcaccgg cagttgcgcc ttgttgcggt aaccgcgggg ttcgtccatg
     8821 acgaccaccg gatcgacgga aacctcgtcg aacggggtgt agcgcgcgaa cgcctcttgc
     8881 accagctccc gtttcaaccg cttttgcatg cgccggtcga tgtgcatcag ttgacacgcc
     8941 ccgcattcgc gatagacggg acattccggt ttcgtccggt aggcggactt cttgttcacc
     9001 cggatgatcc gggcacgggc atgacgcttg tccacctccg tcacccgggc cgtcaccgtt
     9061 tcgcccggca cggctccttc cacgaacacc acctgtttgc ggaaatatcc cacgccctcc
     9121 ccgttgatgc cgatccggcg aacggggagt tcgatcactt ctcctttttt cagtcgcatc
     9181 cgatatccag acatatccaa aaaccctttc cggttcaggc tgttttcggg aaagcttccg
     9241 ctcatgatcc ggagaggggg accgtcctcc cgtccctcag acgggcatgc cggctcaagc
     9301 ggatgtgatc cttcccggtt catcgctttc ggaaactccc ggatttattc tacccgttga
     9361 cagtcaccgg ggaaagtgat ggaacgaacc cccggggact taaggaaaac ccggcccccc
     9421 gattccgtga acgattgggc ggccgggcat ttgccgaaca ggatgtgcac atccgcggga
     9481 tttgatgtcg ggaagtggcc ttcggttggc catcttggaa taaccgcaaa acggtcccgc
     9541 ttttcccttc cgtatcggaa agcgggaccc tccttcactc aaccgggtga atcagatgtt
     9601 ctccctccgc cttcggggaa ttgacccggg tcgacaccgg gtacatttcc aggtccggat
     9661 cgggaaccga catgagcagc tcccggtaaa caccgggatc ggtgtcctgc atgtccagcc
     9721 acatcgtttc cgtttgcggc gtcagaatga cgggcatgcg atcatgaaca tcccgcgccc
     9781 gttcgttcgc ttcggtggtg atgatcgtgc aggaacggat cacctgaccg tcccgctccc
     9841 actcatccca caagccggca aacgaaaaca gttttcctcc ggggagccgg atcaggtacg
     9901 gctgcttttt gcctgatacc gccttccatt cgtagtaacc cgtggcggga atcagacagc
     9961 ggcgccgttt cagcaaccgc cgccacgacg gtttttccgc cagtgtttcc gcccgcgcat
    10021 tgatcatccg gaaacgggga tgaggcgagt cggaccatgc ggggaccaac ccccagcgaa
    10081 accagtcggc tttcatcgtt ccctcatccc tcatgaccgt cagcacgggc tgggtgggcg
    10141 cgatgttgta ccggggagga acctcctccg gcaatgcctc caacagaaag cgctccatca
    10201 attcccgttt gtccgcatcc agcacaaatc ggccgcacat ggcgattcct cccccggaca
    10261 cccgaacgcg accgggcgtc ccgatttcct cgctcaaaca atccggttgc gaagcactcc
    10321 gatgcgttcg atctccagtt caacgacatc cccgggctcc agccagcggt gaacctcctg
    10381 ccccagttcc agaatgcaac cggtgcccac cgttcccgaa ccgatcacgt cgccgggcaa
    10441 caattcacag tcccttgatg cccgttcgat catctgggcg aaactccagt aaagatcctt
    10501 catgttgcct cgagacactt ccgttccgtt gatgcgagcc gtcatggtca gatcccaatg
    10561 ttcccccacc ctccggtctt ccagttcatc gagcgtcacg agccacggtc cgagagacgt
    10621 ggcgaaatct tttccttttg cgggccccaa accgaccgcc atttccttgc gctgctcatc
    10681 acgggcgctc cagtcgttca tcacggtgaa tcccgcgatg tgctccaggg cgcgttcacg
    10741 cggaatgtcc cgcccccgct tgccgatgac gcacgcgatt tcaagttcaa aatcaagcct
    10801 gtccgtgtat gagggcttct tcaccggatc atccggtccg aagaccgaac gg
//
