LOCUS       NZ_QAJN01000017        10876 bp    DNA     linear   CON 23-OCT-2024
DEFINITION  Paucibacter sp. KBW04 KBW4_TGACCA_contig_17, whole genome shotgun
            sequence.
ACCESSION   NZ_QAJN01000017
VERSION     NZ_QAJN01000017.1
KEYWORDS    WGS; RefSeq.
SOURCE      Paucibacter sp. KBW04
  ORGANISM  Paucibacter sp. KBW04
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Roseateles.
REFERENCE   1  (bases 1 to 185028)
  AUTHORS   Lee,F., Williams,K.B., Levin,M. and Wolfe,B.E.
  TITLE     The microbiome of the planarian worm Dugesia japonica mediates
            outcomes of regeneration via indole production
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 185028)
  AUTHORS   Wolfe,B.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-APR-2018) Biology, Tufts University, 200 Boston Ave,
            Medford, MA 02155, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2018
            Assembly Method                   :: CLC Workbench v. 8.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 86.4015849673874x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003852425.1-RS_2024_10_23
            Annotation Date                   :: 10/23/2024 03:09:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,942
            CDSs (total)                      :: 4,879
            Genes (coding)                    :: 4,851
            CDSs (with protein)               :: 4,851
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 3 of 28
            Pseudo Genes (incomplete)         :: 26 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 3 of 28
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:40:34
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 32226
            Orig. end                         :: 43102
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            QAJN01000017.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10876
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10876
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10876
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5876](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5876)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(252..268)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: medium, score: 26.24"
                     /tool="antismash"
     gene            1130..1603
                     /gene="ybgC"
                     /locus_tag="DBR47_RS20710"
                     /old_locus_tag="DBR47_20700"
     CDS             1130..1603
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene="ybgC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 4HBT_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521903.1"
                     /locus_tag="DBR47_RS20710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBR47_20700"
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="WP_124453671.1"
                     /sec_met_domain="4HBT_2 (E-value: 6.5e-18, bitscore: 58.8,
                     seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLLFQHSLRIYWEDTDAGGVVFYANYLKFFERARTEWLRSLGFE
                     QEAMRHQERVMFIVGSTQVKYLRPARLDDWLRITVEARELGRASMVLFQQAWRGETLLA
                     EGEIRIGCVRSNVEAENSPRTSPAAEPLDFKPCRIPASLIEAIENPTKPAQSS"
     gene            1600..2301
                     /gene="tolQ"
                     /locus_tag="DBR47_RS20715"
                     /old_locus_tag="DBR47_20705"
     CDS             1600..2301
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="tolQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541940.1"
                     /locus_tag="DBR47_RS20715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBR47_20705"
                     /product="protein TolQ"
                     /protein_id="WP_124453673.1"
                     /transl_table=11
                     /translation="MNQDLSIVALILHASLPVQLVIAALLFMSLASWSVIFSKFTAISR
                     INSGNEAFEQEFWSGKNLNELYNGVANKTEGAPLERIFASGMREFLKLRERRVTEPTAL
                     LDGARRAMRASFQRELDAMESNLSFLASVGSVSPYVGLFGTIWGIMHAFVGLSNLQQVT
                     LATVAPGIAEALVATAIGLFAAIPAVLAYNRFARQIDRSAITLESFIEEFSNILQRNAS
                     QPIAGTGAGTR"
     gene            2323..2772
                     /gene="tolR"
                     /locus_tag="DBR47_RS20720"
                     /old_locus_tag="DBR47_20710"
     CDS             2323..2772
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /GO_process="GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="tolR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830537.1"
                     /locus_tag="DBR47_RS20720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBR47_20710"
                     /product="protein TolR"
                     /protein_id="WP_124453675.1"
                     /transl_table=11
                     /translation="MAAISSRGSRRRRTINEINMVPFIDVMLVLLIIFMVSAPLITTGL
                     VDLPSVGRSKTQPQHVIHVIVGADEKLKIKLDKQEPESLQINRLAERVKSAQAGEATTP
                     VVISADKSVKYEAVVKVMDILQSAGIQRVGLAVRNGPAESSEHKP"
     gene            2778..3734
                     /gene="tolA"
                     /locus_tag="DBR47_RS20725"
                     /old_locus_tag="DBR47_20715"
     CDS             2778..3734
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /gene="tolA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518836.1"
                     /locus_tag="DBR47_RS20725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBR47_20715"
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_124453677.1"
                     /transl_table=11
                     /translation="MTSPSLSPAFAPDPLRPPDNPGLGRGFAIAAIAHAALLAALSAGV
                     HWRSETPPAFEAELWSAVPQAAAPKEQLPPEPEPEAEPTPPPKPVLAPPPPPPAEDLQA
                     EREAEIAIAKAKEKKKQEQLEAKREAEREALRKKEELAKKEKEKEREKEREKAEKKAAE
                     EKEKQKQEKLEKQKQDKLAKEAAAKKDAAREAKAEAQRQENLRRIQGMAGATGAPNATG
                     SALQSSGPSANYAGRIKARVRPNIIYPDTGAANPVAEVEVRTAPDGNIISRKILKASGD
                     TEWDNAVLRAIDKTAVLPRDTDGRVPSVLIISFKPRE"
     gene            complement(3735..4238)
                     /locus_tag="DBR47_RS20730"
                     /old_locus_tag="DBR47_20720"
     CDS             complement(3735..4238)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862664.1"
                     /locus_tag="DBR47_RS20730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBR47_20720"
                     /product="DoxX family membrane protein"
                     /protein_id="WP_231512423.1"
                     /transl_table=11
                     /translation="MNMALQYLALAARVFCDPGRLSLRSRELPAWLSGGLGAAQSLALL
                     LARLFVAQVFFLSGLTKLRDWSVTLALFQDEYQVPLLPPELAAYLGTAGELALPVLLVL
                     GLGGRFAALGLSVVNLMAVLSLAEIAPAALLGHQLWGVLLLLLGLFGAGRLSVDEFLFR
                     PNRG"
     gene            complement(4238..5014)
                     /locus_tag="DBR47_RS20735"
                     /old_locus_tag="DBR47_20725"
     CDS             complement(4238..5014)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021364.5"
                     /locus_tag="DBR47_RS20735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBR47_20725"
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="WP_124453679.1"
                     /transl_table=11
                     /translation="MSRLELQADQAALVAALSSSTVEVQGLLGLGPAAAGLRRGLLAYR
                     VNAQALACRALAAVYSPLRDWLGAASFEGLAWAFWRASPPQRGDLAEWGEGLADFLAAQ
                     AEMEALPCQLARLCWAAHRVEAARDAELDAPSLALLAQREPRDLGLRLRPGVCLVQASP
                     AALACWRGAPEAEIEAGEEQACPVLVWRQAWVARARVLAAGEHVFLQALLAGEDLDTAL
                     HLAFSAQADFDFGICLQTLLQEDCLWAAYEIETKES"
     gene            complement(5001..5876)
                     /locus_tag="DBR47_RS20740"
                     /old_locus_tag="DBR47_20730"
     CDS             complement(5001..5876)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912929.1"
                     /locus_tag="DBR47_RS20740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBR47_20730"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_124453681.1"
                     /sec_met_domain="DUF692 (E-value: 5.7e-75, bitscore: 245.4,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLGNSNLPVGIGWRQPHYQQLMLERPALGFLEVHSENFFAEGGAA
                     LSLLSEAREHYEISLHGVGLSLGSACGLDAWHLDHLAQLVERVQPCRVSDHASFARVPR
                     PGGAGFQHASDLLPIAFSPASLDLMVAHVQEVQERLRRPLLVENLSAYLAWEVDEAMSE
                     PEFFNQLARRSGCGLLLDINNLFVNARNSGAARPEALQQVCAWMDALRPEAVAEVHLAG
                     HAELEDLVIDDHGSRVCAEVWQAYRHGLRRFGSVPTLIEWDTDLPPLSVLLDEARLAAR
                     AMEGLQHEPA"
     gene            complement(5889..6191)
                     /locus_tag="DBR47_RS20745"
                     /old_locus_tag="DBR47_20735"
     CDS             complement(5889..6191)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830542.1"
                     /locus_tag="DBR47_RS20745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBR47_20735"
                     /product="DUF2282 domain-containing protein"
                     /protein_id="WP_124453683.1"
                     /transl_table=11
                     /translation="MKTNVLIASALASVCALGLLSPASAAEEKAAKEKCYGISKAGQND
                     CANLSGSHSCAGQATAAMTPDEWKYVPKGSCKSLKGLSAAEAKAAMPAAAAAKKS"
     misc_feature    complement(6177..6192)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.51"
                     /tool="antismash"
     misc_feature    complement(6294..6309)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 20.11"
                     /tool="antismash"
     gene            6318..6926
                     /locus_tag="DBR47_RS20750"
                     /old_locus_tag="DBR47_20740"
     CDS             6318..6926
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347556.1"
                     /locus_tag="DBR47_RS20750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBR47_20740"
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_124453685.1"
                     /transl_table=11
                     /translation="MTEPATKAIQAEMAPQLEKLRPQLLKYATLQLRNAAWAEDAVSDT
                     LLAALERPQSFAGQSQLKTWLIGILKHKLVDQIRRNSKEMSTTAEDGQDLDEDLFHPNG
                     HWRETPQDWGDPEHCLRQLDFMRVLEACVEHLPGQQGRLFMMREWLELDTEEICKALDI
                     SATNLWVMLHRARLRLRECLQLNWFGARAPQTQEQGGAL"
     gene            6923..7114
                     /locus_tag="DBR47_RS20755"
                     /old_locus_tag="DBR47_20745"
     CDS             6923..7114
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519893.1"
                     /locus_tag="DBR47_RS20755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBR47_20745"
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="WP_197047202.1"
                     /transl_table=11
                     /translation="MKLRPNCREVTELVLLAQEKPLSLGERVAVRMHMLICKACPRFLK
                     QVQLMRQASERWRHYSEE"
     gene            7245..9785
                     /locus_tag="DBR47_RS20760"
                     /old_locus_tag="DBR47_20750"
     CDS             7245..9785
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552452.1"
                     /locus_tag="DBR47_RS20760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DBR47_20750"
                     /product="GspE/PulE family protein"
                     /protein_id="WP_231512424.1"
                     /transl_table=11
                     /translation="MPDLSAPLNWPHGEAPAGSAEANNATGKLNFVWPMPPFAAYPRAK
                     AQSTPEPCEVLGLNGSRNPGHLILMAPQDRVAQVLVPPARNAMPLKFGQFRALKLLRPI
                     VAAPREIDPADDVLKLDSRPRSEFKLFFVGGGELKGRTIGHCQDDLGLFLFPPISDADD
                     SVYRVFVPREVLDHFEIGERIGDVLVKENLATAAQINEAASEQQSLRSRRVGDILVAES
                     IVSAEQLMIAIEQQARMPMVRIGEALLALELITEEQLEHALELQRGDRSVPLGELLVRK
                     GIVTRQALQSALARKMGYPIVDVASFAIEPDAVKKIPYAVAKRLDVLPLLLRDGRLIVA
                     MEDPTRREALDEIEFIAQLKVTPTLTKVGTLLSAIPSTYERYGGDAIARSELTPGSYQA
                     IDFSLDKADQLIETLEREVQDRGGRDEEVPLEQSDNSLVRLINTMIIEASSQGVSDIHI
                     ETYPGKEKLKIRFRKDGVLKPYLELPNTYRNAMIARIKIMCELDISERRKPQDGKINFA
                     RFSPQHKLELRVATIPTNNGLEDVVMRLLASSKPIPLEKLGLTTRNLSELKKAVSRPYG
                     MVLCVGPTGSGKTTTLHSAMSYINTPDRKIWTAEDPVEITQAGLRQVQVNPKIDWTFAK
                     ALRAFLRADPDVIMVGEIRDEETGEIAVEASLTGHLVMSTLHTNSAAETITRLLDMGMD
                     PFNFADSLLAVLAQRLVRRLCPSCRTSKPIAEAGLQEWLDDYLHIYPNEQRPSRQSVLD
                     DWVKSYGHKGQILHYHSPGCEVCDQTGYKGRAGLHELLVVSKVLRRQIQTGARAEELLQ
                     TALAEGMQSLRQDGILKVLQGITTMAEVRATSNA"
     misc_feature    complement(7246..7262)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.22"
                     /tool="antismash"
ORIGIN
        1 gcggcccctt gtgcgacccg gcccagggcg gccggcgcat gatttacctg aatatcggcg
       61 aacccgactt cggcgccccg cccatggtgc aagccgccgc ccagcgctgc atcgaagcag
      121 gccgcaccca atacacctcg gccacaggcc tgccggaatt gcgccaggcc ttgtcggact
      181 ggtatcaaca gcactttggc ctcgccataa cgcccgatcg catcgtcatc acggccggtg
      241 cctcggccgg cttgcaactc gtttgccaag cattgttcga ggcgggcgac gaggtgttga
      301 tgcccgaccc ctgctatccc tgcaaccgcc atttcgtggc cggcacggac gcgacgccgg
      361 tgctgctgcc caccggcccc gagcaacgct tccagcccag cagcgcacag atccgccagg
      421 cctggaacga acacacccga gccgtcttgc tggcctcacc cagcaacccc accggcacct
      481 cgattgagcc ggccgagctg cgcgccatcg tggaagcggt gcgcgagcgc gggggcctca
      541 ccatcgtcga cgaaatctat ctgggcctga gctacgaccc gcgctttgaa gcctcggctt
      601 tgggcttggg tgacgatgta atctcggtca acagcttttc caaatacttc ggcatgaccg
      661 gctggcgcct gggctggctg gtgctgccgc cggccttggt ggcgccgatc gagaagctgg
      721 cgcagaactt cttcatctgc ccctccagca tcgcccagca tgccgccctg gcctgcttcg
      781 agcctgccgc aatggccatt tatgagcagc gccgggagga gttccgtgcc cggcgcgact
      841 acatcgtgcc ggccttgcaa gccctgggcc tgcaggtgcc ggttccgccc gatggggctt
      901 tctacgtctg gatggattgc agcgagttct gcgccaatag ttgggacctt gctttcgagc
      961 tgatgcgtag cgcccaaatt gcgctgacac ccggccgcga ttttggccgc cagggagccg
     1021 agcgctggct gcgcctgtct ttcgccagca gcatggaaga tttgcaagca accgtcgccc
     1081 gcctggccga ggtactgcct cgctaccgcg ccaaccccac tcacaccgaa tgagcttgct
     1141 gttccaacat tccctgcgca tctactggga agacaccgac gccggtggcg tggtcttcta
     1201 tgccaactac ctgaaattct tcgagcgtgc ccgcaccgaa tggctgcgca gcctgggctt
     1261 tgagcaagaa gccatgcggc accaggagcg ggtgatgttc atcgtcggca gcacgcaagt
     1321 gaagtatctg cggccggcgc gcctggacga ttggctgcgc atcaccgtcg aggcacgcga
     1381 actgggccgg gccagcatgg tcttgttcca gcaagcctgg cgcggcgaga ccttgctggc
     1441 cgaaggcgag attcgcatcg gctgcgtgcg cagcaatgtc gaggccgaga acagccctcg
     1501 cacgagcccg gccgccgagc cactagactt caagccctgc cgtattccgg cctcgctgat
     1561 cgaggccatc gagaacccga ccaagcccgc ccaaagctca tgaaccaaga cctgtccatc
     1621 gtcgccctga tcctgcatgc cagcctgccc gtgcaactgg tgattgccgc cttgctcttc
     1681 atgtccttgg ccagctggag cgtgatcttc agcaagttca ccgccatctc gcgcatcaac
     1741 agcggcaacg aggcttttga gcaggagttc tggtcgggca aaaacctcaa cgagctttac
     1801 aacggcgtgg ccaacaagac cgagggcgca ccgctggagc gcatcttcgc ctccggcatg
     1861 cgcgagttct tgaagctgcg tgaacgccgc gtgaccgagc ccaccgcctt gctggacggc
     1921 gcccgccgcg ccatgcgcgc cagcttccag cgcgagctgg atgcgatgga aagcaatctc
     1981 tccttcctcg cctcggtcgg ttcggtctcg ccctatgtgg ggctgttcgg caccatctgg
     2041 ggcatcatgc acgccttcgt cggcctgtcc aatctgcagc aggtgaccct ggccacggtg
     2101 gcccccggta ttgccgaagc cctggtggcc acagccatcg gcctgttcgc cgctattccc
     2161 gccgtgctgg cctacaaccg ctttgcgcgc cagatcgacc gcagcgccat caccctggag
     2221 tccttcatcg aggagttctc caatatcctg caacgcaacg ccagccagcc tatcgcaggc
     2281 accggcgctg gcacgcgcta agcagcggct caggtcatcg ccatggctgc catcagctcg
     2341 cgcggctcgc gccgccgccg taccatcaac gaaatcaata tggtgccctt catcgatgtg
     2401 atgttggtgc tactcatcat cttcatggtc agcgcgcctc tgatcaccac cggcctggtg
     2461 gacctgccca gcgtgggccg cagcaagacc cagcctcagc atgtgatcca cgtcatcgtg
     2521 ggcgccgacg agaagctcaa gatcaagctg gacaagcagg aacccgagtc cctgcaaatc
     2581 aaccgtttgg ccgagcgggt caagtccgcc caggccggtg aagcgacgac gccggtggtg
     2641 atctcggccg acaaaagcgt caagtacgag gccgtcgtca aggtgatgga cattctgcaa
     2701 agcgccggca tccagcgcgt cggcctggcc gtgcgcaacg gcccggcgga aagcagcgag
     2761 cacaagccct gattggcatg acgagcccca gcctgagccc cgccttcgcg cccgatcccc
     2821 tgcgcccgcc ggacaatccc ggcctgggcc gcggcttcgc cattgccgcc atcgcccacg
     2881 ccgccctgct ggccgcgctg agcgcgggtg tgcactggcg ctccgaaacc ccgcccgcct
     2941 tcgaggccga actgtggtcg gccgtgcccc aagccgcggc acccaaggaa caactccctc
     3001 ccgagcctga gcccgaagcc gagcccacgc cgccgcccaa gccggtgctc gcgcctcctc
     3061 cgcccccgcc cgccgaagac ttgcaagccg aacgcgaggc ggaaatcgcc atcgccaagg
     3121 ccaaggaaaa aaagaagcag gagcagctag aggccaagcg cgaggccgaa cgtgaggccc
     3181 tgcgcaagaa ggaagagttg gccaaaaagg agaaagaaaa ggaacgcgag aaagagcgcg
     3241 agaaggccga gaagaaggca gccgaagaga aagaaaaaca aaaacaagaa aagctggaga
     3301 agcaaaaaca ggacaagctg gccaaggaag ccgccgccaa aaaggacgcc gcccgcgagg
     3361 ccaaggccga agcccagcgc caggaaaacc tgcgccgcat tcagggcatg gcaggcgcga
     3421 ccggcgcgcc gaacgccacg ggctcggccc tgcagtcctc cggcccctcg gcgaactatg
     3481 ccggccgtat caaggcgcgc gtgcgcccca acatcatcta ccccgacacg ggcgccgcca
     3541 acccggtggc cgaggtcgag gtgcgaaccg cgccggacgg caacatcatc tcgcgcaaaa
     3601 tcctcaaggc cagcggcgac accgaatggg acaacgccgt gctgcgcgcc atcgacaaga
     3661 cggccgtgct gccgcgtgac accgatggtc gcgtgccctc ggtgctcatc atcagcttca
     3721 agccacgcga gtgattagcc tctgttaggc cggaacaaga attcatccac agagagacgc
     3781 cccgccccga acaagcccag cagcaggagc aagacgcccc agagctgatg gccaagcagg
     3841 gccgccgggg cgatctcggc aaggctgagt accgccatca gattgaccac gctcaggccc
     3901 agggcggcga agcgcccgcc caggcccagc accagcaaga cgggcaaggc cagctctccg
     3961 gccgtgccca ggtatgccgc cagctcgggc ggcaagaggg ggacttggta ctcgtcctga
     4021 aacaaggcca gggtgacgga ccaatcgcgc agcttggtca ggcccgagag aaaaaagact
     4081 tgcgctacaa acagccgggc cagcaagagg gccagagact gggccgcgcc cagccctccg
     4141 ctcagccaag ccggtagttc gcgcgaacgc aaggagagcc gccccgggtc gcagaaaacg
     4201 cgtgccgcga gcgctaggta ttgcaaagcc atgttcatca tgattccttg gtttcgatct
     4261 cgtaagccgc ccataggcag tcctcttgca gcagggtttg caggcaaatg ccaaagtcaa
     4321 aatcggcctg agcgctgaag gccaggtgca aggcggtgtc caggtcctca cccgccaaca
     4381 aagcctgcaa aaaaacatgc tcaccggcag ccaagacgcg agcacgggcc acccaggcct
     4441 gacgccagac cagcacgggg catgcttgct cctcgcccgc ttcgatctcg gcctccggcg
     4501 cgccgcgcca gcaggccaag gccgcggggc ttgcttgcac caggcagacg cccggccgca
     4561 gtcgcaggcc caggtctcgc ggctcgcgct gagccaacag tgccagcgag ggcgcatcca
     4621 gctcggcgtc ccgcgcggct tcgacccggt gcgcggccca gcacagacgg gctagctggc
     4681 aaggcaaagc ctccatctcg gcttgggcag ccaaaaagtc cgccaggccc tcgccccatt
     4741 ccgccaagtc gccccgctgc ggcgggctgg cgcgccaaaa ggcccaggcc aagccctcga
     4801 aggacgctgc acccagccag tctcgcaagg ggctgtagac ggcggccaag gcccggcaag
     4861 ccagggcttg ggcattgacg cgataggcca gcaaaccgcg cctcaagccc gcagccgccg
     4921 ggcccaagcc cagcaaaccc tgtacctcga cggtgctgga actcagggcc gcgaccaagg
     4981 ccgcctgatc tgcctgcagc tcaagccggc tcatgctgca agccctccat ggcccgcgcg
     5041 gccaggcgcg cctcgtccag caagacgctc aagggcggca gatcggtatc ccattcgatc
     5101 agggtcggca cggagccgaa gcgtcgcagt ccgtgacggt aggcctgcca gacctcggcg
     5161 cagacgcggc tgccgtgatc gtcgatcacc aagtcctcca gctccgcatg gccggccaga
     5221 tgaacctcgg ccacggcctc gggccgcagc gcgtccatcc aggcacacac ttgttgcagc
     5281 gcctcgggcc gagccgcgcc gctgttgcgg gcattgacga agaggttgtt gatgtccagc
     5341 agcaggccgc aaccgctgcg ccgcgccagt tgattgaaga actcaggctc gctcatggcc
     5401 tcatccacct cccaagccag ataggcgctg aggttctcca ccagcagggg ccggcgcaaa
     5461 cgctcctgca cctcttgcac atgagccacc atcaggtcca ggctggcagg gctgaaggcg
     5521 atgggcagca gatcgctggc atgttgaaaa ccggcgccgc ccggcctagg cacacgggca
     5581 aagctggcgt gatcagacac ccggcagggc tggacgcgct ccaccagctg agccaaatgg
     5641 tccagatgcc aggcgtccaa gccacaggcc gagcccagcg acaagccgac gccatgcagg
     5701 ctgatctcgt aatgctcgcg cgcctcgctc agcaggctca gggcagcgcc gccctcggcg
     5761 aagaagttct ccgaatgcac ttccagaaag cccagggccg gccgttccag catcagctgc
     5821 tggtagtgag gctggcgcca gcctatgccc accgggagat tcgagttgcc gagcatggcg
     5881 ggtctgcctc aggacttctt ggcggctgcg gcagcgggca tggcggcctt ggcctcggca
     5941 gcgctcaggc ctttgaggct tttgcagctg cccttgggca catacttcca ttcatccggc
     6001 gtcatcgccg ccgtggcctg accggcgcag gagtggctgc cgctcagatt ggcgcagtcg
     6061 ttctgcccgg ccttgctgat gccataacat ttttctttgg cggccttttc ttcggcggcc
     6121 gaggcgggcg acaacagccc cagggcgcaa acagaagcca gagcggaagc gatcaagaca
     6181 ttggttttca tcagggactc caagatttgg gattcaaaaa agtcgggcgg cttgtgcggc
     6241 ccgcatccga cagctttgtg tcgcaacaag gcccgctgtc cttacactgc gttctgaaaa
     6301 aattctcgaa agcgcgcatg accgagcccg caacaaaagc gattcaggcc gagatggccc
     6361 cgcagctgga gaagctcagg ccccagttgc tcaagtacgc cacgctgcag ttgcgcaacg
     6421 ccgcctgggc ggaggacgcg gtgtccgaca ccttgctggc cgcgctggag cggccgcaga
     6481 gttttgccgg tcagtcccag ctcaagacct ggttgatcgg catcctcaag cacaagctgg
     6541 tcgatcagat ccgccgcaat agcaaggaga tgtcgaccac ggccgaagat ggtcaggacc
     6601 tcgacgaaga tctcttccat cccaacggcc attggcgcga gacgccgcag gactggggcg
     6661 accccgagca ttgcctgcgc cagctggact tcatgcgggt gctggaggct tgcgtggaac
     6721 atctgcccgg tcagcaaggg cgtttgttca tgatgcggga atggctggag ctggacacag
     6781 aagaaatctg taaggctttg gacatcagcg cgaccaatct ctgggtcatg ctgcacaggg
     6841 cccggctgcg cctgcgcgaa tgcctgcagc tcaactggtt cggggcacgg gccccacaaa
     6901 cccaagaaca aggaggggcg ctgtgaagct caggcccaat tgccgcgagg tgaccgagct
     6961 ggtcctgctg gcccaggaaa aacccctctc gctgggcgag cgtgtggccg tgcgcatgca
     7021 tatgctgatc tgcaaggcct gcccacgttt tctcaaacag gtgcagctga tgcgccaggc
     7081 cagcgagcgc tggcgccact acagtgaaga gtgaagctgt aggctctgtg cccgccaatt
     7141 cagcgctttg acgtggccct caggcagtag catcgaaggc gcaaagagcc gtagagccgg
     7201 gacgtcgctc aaacgttcca ctcaaaccct gtccggagga atgaatgccc gatttgtctg
     7261 ccccgctgaa ctggccccac ggcgaagccc ctgccggctc ggccgaagcc aacaatgcca
     7321 cgggcaaact caatttcgtc tggcccatgc cgccctttgc ggcctacccg cgcgccaagg
     7381 cacaaagcac gcccgaaccc tgcgaggtct tgggcctgaa tggcagccgc aatcccggcc
     7441 atctgattct gatggcgccc caagaccggg tggcccaggt gctggtgcca ccggcccgca
     7501 acgccatgcc gctcaagttc gggcagttcc gcgccctcaa gctcttgcga cccatcgtcg
     7561 cggcgccgcg tgaaatcgac ccggccgacg atgtgctcaa gctggattca cgcccacgca
     7621 gcgagttcaa gctctttttc gtgggcggcg gcgaactcaa gggccgcacc atcggccatt
     7681 gccaggacga cttaggcctc tttctcttcc cacccatcag cgatgcggac gactcggtct
     7741 accgcgtctt cgtcccccgc gaggtgctgg accatttcga aatcggcgag cgcatcggcg
     7801 atgtgctggt gaaagaaaac ttggccaccg ccgcccagat caacgaagcc gccagcgagc
     7861 agcaaagcct gcgctcgcgc cgcgtcggcg acattctggt ggccgagagc atcgtcagcg
     7921 ccgagcagct gatgatcgcc atcgagcagc aggcccgcat gcccatggtg cgtatcggcg
     7981 aggccttgct ggccctggag ctgatcaccg aggagcagct ggagcacgca ctggagctgc
     8041 aacgcggcga ccgctccgtg cccctgggcg agctgctggt gcgcaaaggc atcgtcaccc
     8101 gccaggcctt gcagtccgcc ctggcgcgca agatgggcta ccccatcgtc gatgtggcca
     8161 gctttgccat cgaacccgac gcggtcaaaa aaatccccta tgccgtggcc aagcgcctgg
     8221 acgtgctgcc tctgctgctg cgcgatggcc gcttgatcgt ggccatggaa gacccgaccc
     8281 ggcgcgaggc cctggacgag atcgagttca tcgcccagct caaggtcaca cccacgctga
     8341 ccaaggtcgg caccttgctc agcgccatcc caagcaccta cgagcgctat ggcggcgacg
     8401 ccatcgcccg cagcgagctg acgcccggca gctaccaggc gatcgacttc tcgctggaca
     8461 aggccgatca gctgatcgag accctggagc gcgaggtgca ggaccgcggg ggccgcgatg
     8521 aggaagtgcc gctggagcag agcgacaact ccctggtgcg tctgatcaac accatgatca
     8581 tcgaggccag cagccagggc gtgtccgaca tccatatcga gacctatccg ggcaaagaga
     8641 agctcaagat ccgcttccgc aaggacggcg tgctcaagcc ctatctggag ctgcccaata
     8701 cctaccgcaa cgccatgatc gcgcgcatca agatcatgtg cgaactcgat atctccgagc
     8761 gccgcaagcc gcaggacggc aagatcaact tcgcccgctt ctccccccag cacaagctgg
     8821 aactgcgggt cgccaccatc cccaccaaca acggcttgga agacgtggtg atgcgcttgc
     8881 ttgcctcgtc caaacccatc ccgctggaaa aactgggcct gaccacccgc aatctgagtg
     8941 agttgaagaa agccgtcagc cgcccctacg gcatggtgct ttgcgtgggc ccaaccggct
     9001 cgggcaagac caccaccctg cactcggcga tgagctatat caacacgccg gaccgcaaga
     9061 tctggacggc cgaggacccg gtggaaatca cccaggccgg cctgcgccag gtccaggtca
     9121 accccaagat cgactggacc tttgccaagg ccttgcgcgc atttttgcga gccgacccgg
     9181 atgtgatcat ggtgggcgaa atccgcgacg aagagaccgg cgagatcgcc gtcgaggcct
     9241 cgctgaccgg ccacctggtg atgtccaccc tgcacacgaa tagcgcggcg gaaaccatca
     9301 cccgtctgct ggacatgggc atggacccct tcaacttcgc cgactccctg ctggccgtgc
     9361 tggcccagcg cctggtgcgg cggctgtgcc cctcctgccg aacttccaag ccgattgccg
     9421 aggccggcct gcaggaatgg ctggacgact atctgcacat ctacccgaat gagcagcgcc
     9481 cctcgcgcca aagcgtgctg gacgattggg tcaagagtta tggccataag gggcaaatcc
     9541 tgcattacca cagccccggc tgcgaggtct gcgatcaaac cggctacaag ggccgggccg
     9601 gcctgcatga gttgctggtg gtctccaaag tgctgcgccg ccagatccag accggcgctc
     9661 gcgccgaaga gctgttgcag acggccctgg ccgagggcat gcaatccctg cgccaggacg
     9721 gcatcctcaa ggttctgcaa ggcatcacca ccatggctga ggttcgcgcg acgagcaatg
     9781 cctgagtcca catcgacaag agaaacaagc aaaaccgacg atggatacag aaaacagcgc
     9841 aagcaatgct gacgcgggct cgggcccacc aaaaaaagtg cgcctgggcg atgtgctggt
     9901 gcagcagcag ctgatctccg aagcccagtt gcaacaagcc ctggagctgc agcgcagctc
     9961 aggaaagaag ttaggccggc tgctgattga agccggcctg atcaccgaag aggccctggc
    10021 ccatgtgttg gcgcgccagc tgcgcattcc cttcgtcaat ctcaagacct ttccgctcaa
    10081 gaccgatctg gtgcgactgc tgtcggagac cccggcgcgg cgctttcgcg ccatcgtgct
    10141 ggaagaccgc ggcgaggcct tgttggtggc catggccgac ccgctggacc tgtttgccta
    10201 cgacgagctg agccgcctgc tcaagcgccg catcaatatg gccgtggtgg ccgaaagcca
    10261 gctgcccacc gcttttgacc gccactaccg ccgcagcgag gaaatcagcg gcctggccaa
    10321 ggcgctggag aaagacatcg gcgacgcggt ggacttcggc gccctgcaag ccagcgtggg
    10381 ccaggaaggc gcgccggtgg tgcgcctgct gcaaagcgtg tttgaggacg cgacccaggc
    10441 gggggcctcc gatgtgcaca tcgagccgca agaaggcgag ctgctgatac gcacccgcat
    10501 cgacggcgtc ttgcaaaccc aaacgcaggc cgacaagcgc attgccgccg ccctgtccca
    10561 gcgcctcaag ctgatggcag gtctggacat ttccgaaaag cgcctgccgc aggacggccg
    10621 cttcaccctg cgcctgcgcg agcgcaccat cgacgtgcgc ctgtccaccc tgcccggcca
    10681 gtacggcgaa tcggtggtga tgcggctctt gggccagggc aatgcgattc gccgcctgca
    10741 ccagatcgag atgcaggccg acatgctggc gcgctttcgc gagatcttgt cccagcagtc
    10801 cggcatggtg ctggtcactg gccccaccgg ctcgggcaag accaccacgc tctacgctgc
    10861 actcagtgag ctgaac
//
