LOCUS       NZ_ML123034            10522 bp    DNA     linear   CON 23-MAY-2024
DEFINITION  Streptomyces sp. ADI96-02 scaffold001, whole genome shotgun
            sequence.
ACCESSION   NZ_ML123034
VERSION     NZ_ML123034.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. ADI96-02
  ORGANISM  Streptomyces sp. ADI96-02
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 6907715)
  AUTHORS   Guerrero-Garzon,J.F., Zehl,M., Urban,E., Schneider,O., Ruckert,C.,
            Busche,T., Kalinowski,J. and Zotchev,S.B.
  TITLE     Streptomyces spp. from the marine sponge Antho dichothoma: analyses
            of secondary metabolite biosynthesis gene clusters and some of their
            products
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6907715)
  AUTHORS   Guerrero-Garzon,J.F., Zehl,M., Urban,E., Schneider,O., Ruckert,C.,
            Busche,T., Kalinowski,J. and Zotchev,S.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2018) CeBiTec, Bielefeld University, Sequenz 1,
            Bielefeld 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64.8x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003846185.1-RS_2024_05_23
            Annotation Date                   :: 05/23/2024 03:48:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,089
            CDSs (total)                      :: 6,032
            Genes (coding)                    :: 5,843
            CDSs (with protein)               :: 5,843
            Genes (RNA)                       :: 57
            tRNAs                             :: 54
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 189
            CDSs (without protein)            :: 189
            Pseudo Genes (ambiguous residues) :: 0 of 189
            Pseudo Genes (frameshifted)       :: 68 of 189
            Pseudo Genes (incomplete)         :: 155 of 189
            Pseudo Genes (internal stop)      :: 10 of 189
            Pseudo Genes (multiple problems)  :: 42 of 189
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:00:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6490816
            Orig. end                         :: 6501338
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            ML123034.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Zotchev Lab, Department of
            Pharmacognosy, University of Vienna.
            Annotated using prokka v1.11 from http://www.vicbioinformatics.com.
FEATURES             Location/Qualifiers
     region          1..10522
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="27"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10522
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10522
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5522](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5522
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    231..245
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
     gene            complement(2078..3343)
                     /locus_tag="EES43_RS28365"
                     /old_locus_tag="EES43_28005"
     CDS             complement(2078..3343)
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964394.1"
                     /locus_tag="EES43_RS28365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_28005"
                     /product="glycosyl hydrolase"
                     /protein_id="WP_124271410.1"
                     /transl_table=11
                     /translation="MPAKRRLISTCVAAVALSLLAGGGIAGQPPPGADAVDSAAPGPPA
                     VPGATAHGAYLDYGPAGVSRMAELSRWLGGADLRVGHTYLPGDLWVNIEGAPGFLTSWA
                     DWRAEDEDRLFVLNVPMLERNEERVPDAEVRSLLRSGAAGDFDRHFTRLAERLVSLGVP
                     DTVVVLGWEMNGTTYTHRCGPDPAAWKAYWQRIVTAMRDVPGQRFRFDFTPSRGRDAVP
                     WTECYPGDDVVDIIGMDSYDQPPGESFDDQIHDPYGLQKHVDFAAAHGKPISFPEWGLF
                     RNGDNPVYMRRMLEWIDRHDPLYQTITDYCPHGVWQCKRNPRSSRVYRTMLTEMAAPAR
                     PATGTPSPAPTPPPAPTAPAPGPSGTPGASEPSPRPSPPVRPAPSSAPPAPATPPRRWC
                     VTVPFADLLGRWLRERRICFRY"
     gene            complement(3469..4758)
                     /locus_tag="EES43_RS28370"
                     /old_locus_tag="EES43_28010"
     CDS             complement(3469..4758)
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964393.1"
                     /locus_tag="EES43_RS28370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_28010"
                     /product="cytochrome P450"
                     /protein_id="WP_124271411.1"
                     /sec_met_domain="p450 (E-value: 3.3e-20, bitscore: 70.7,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVAHRTSGNRVRHAADVDLADPDFWRLPRPDRLRAFARLRELDE
                     PVLFTPGPGAARTSGKPFYALVRHADVRTASRTPQVFASAPGVTTPEPARWARALFGDS
                     MVSMDGVEHAALRRIVSRRFTPRLLAAAEENIGRLAGRLVDEMVQARSGDFMPSAASRL
                     PLEVICDLMGIPGAYRARIAAQIDHASEHVGVARRGRARIRIPGRGLASLARMRLLMAR
                     LAHERRRRPQDDLVSALVCADVDGEALSARRLGAFFSLLLVAGVETTRNALAHGLSLLH
                     RHPEQQALLRSDFDRHIGGAVDEIVRHSTPIIQFRRTVVAEFRLGGRTFRPGERVALVY
                     ASANRDEAVFTRPDIFDITRSPNPHLGYGGGGPHHCLGAHLARLEMTALFREILTRGRV
                     VPRPGDPRLIDANFDNRVGSLPLTFGPAFT"
     gene            5001..5522
                     /locus_tag="EES43_RS28375"
                     /old_locus_tag="EES43_28015"
     CDS             5001..5522
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955806.1"
                     /locus_tag="EES43_RS28375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_28015"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_124271412.1"
                     /sec_met_domain="melC (E-value: 1.7e-46, bitscore: 156.3,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPPAPPTNSPTAPNSAGSPTPATDRADAPPATSGTSRRVALRTAF
                     TAAAVAGAAAVLLPVLDREAGSATRSAPGPAADRPDRGRATERFAETYRGREIRGTATD
                     VVPAGRGLPSGQVTEVLIDGRPLHVMRRADGTYLSEVRHYESYPTLLETARAAVDELGS
                     ARLRAPSHSM"
     gene            5543..6403
                     /locus_tag="EES43_RS28380"
                     /old_locus_tag="EES43_28020"
     CDS             5543..6403
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955807.1"
                     /locus_tag="EES43_RS28380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_28020"
                     /product="tyrosinase family protein"
                     /protein_id="WP_124271413.1"
                     /transl_table=11
                     /translation="MHTRRNQRDLTRTEKRRLVDAILRLKRSGRYDDFVVMHREFYVMD
                     TEGQPRPAHMTPSFFPWHRRYLLEFEKALQAVDPKVSVPYWDWTRDNTPAASLWAEDFL
                     GGNGRAGDREVTTGPFAYRAGNWKVGVGVTDENYLTRNFGRPEDPVALPTKEDVARALK
                     DPVYDTEPWNSTSVKGFRNRVEGWGIHQVRGVANHNRVHQWVGGPMAGAASPEDPVFWL
                     HHSFMDLLWDRWQKAHPRSAYLPGRAPAEPGPGRKTVFGLHDPMPPWNVTPAKLTGHGK
                     LYRYA"
     gene            complement(6484..7632)
                     /locus_tag="EES43_RS28385"
                     /old_locus_tag="EES43_28025"
     CDS             complement(6484..7632)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613017.1"
                     /locus_tag="EES43_RS28385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_28025"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_124271414.1"
                     /transl_table=11
                     /translation="MRARRAGRLSVTLCRDFREFGALAGEWDALHRRCATATPFQSHAW
                     LHSWWISYGQEGRLRVLLVRRSGRLIGAAPLMLAHRPMPLLVPMGGAISDFFDILLDEE
                     EAGYAVGALGRGLERAARHAVVDLREVRPDASAQLLFGRWAGGRSRLTDSTCMELPARP
                     LGDLASPLTGARGQRLRAKLRRVDALGVETRRIPPSEVPAAIGTLLRLHELQWRDRGVN
                     PEHLRTRFSDHLIRSVGRMAGDGTAAMTEFRLDGTVVVANLTLQSGRLAGGYLYGADPG
                     LRERKVDVSTLLLREVAQQASDAGHEVLSLLRGAEQYKDHWRPVRVVNQRLLLARPGLE
                     PLLRLRESQVAVRERAAGTVKSRFPAARDWRDRLAGLPVLGR"
     gene            complement(7629..8306)
                     /locus_tag="EES43_RS28390"
                     /old_locus_tag="EES43_28030"
     CDS             complement(7629..8306)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377858.1"
                     /locus_tag="EES43_RS28390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_28030"
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="WP_124271415.1"
                     /transl_table=11
                     /translation="MTDSPEQRRHVLRRRPRTAVPRPPRWWPLPALVLLGAASGLSYGL
                     LTAPRYEATSYAMAVAQEETDPSAALGYAQSFGRLVTSDATLSYAQGAAGEPVRELRSR
                     VRSETSPDSPMIAVTGTSTQPRRAADIANAVIEAVVVSSEHVSEDTGVKLIEFTHAMTP
                     DQPVSPSAPLGTAVGAAAGGLLGGLVLLVRPRRAGWSVSAQVPGPTASGGQGVPAGDRE
                     LAR"
     gene            complement(8303..9439)
                     /locus_tag="EES43_RS28395"
                     /old_locus_tag="EES43_28035"
     CDS             complement(8303..9439)
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015575905.1"
                     /locus_tag="EES43_RS28395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_28035"
                     /product="glycosyltransferase"
                     /protein_id="WP_124271416.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 7.1e-19,
                     bitscore: 66.5, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRALHVITGLGVGGAEQQLRLLLRHMPVPCDVVTLTNPGAVAAGL
                     RADGVRVVHLGMGGNRDLSAVGRLVRLIRDGRYDLVHTHLYRACVYGRIAARLAGVRAT
                     VATEHSLGRDEIEGRPLTRGVRALYLGTERLGSATVAVSPTVAGRLRDWGVPADRIRLV
                     PNGIDADRFRFEPERRAAVRAALGLPDGVFVVGGVGRLVPGKRFDVLVRAVAALPGARL
                     LLAGDGPEAGRLRALAGRLGAANRIRFLGECGDGRGPVPGVPELLSAVDAFVSVSREES
                     FGLAAVEALAAGLPVLHGACPAIDDLPTAHAPGATRITGCPDELTALLRRRIHAPSCDR
                     LPPPRAVGHYDIRRSSTRLMDVYAYALDTVPPNRRALP"
     gene            complement(9436..10248)
                     /locus_tag="EES43_RS28400"
                     /old_locus_tag="EES43_28040"
     CDS             complement(9436..10248)
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018512954.1"
                     /locus_tag="EES43_RS28400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_28040"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_124271417.1"
                     /transl_table=11
                     /translation="MAADPSAPGSGTAPPRPRPHTRRRPWILTYHSVADPRDDPYGITV
                     SAERLGQQLSWLRSRKLTGVGVAELLRAEAAGRRGLVGLTFDDGYADFLGEALPVLREH
                     GCTATVFVLPGRPGGVNDWDPLGPRKPLLTEEEIRRVAGAGMEVGSHGLLHRRLTALSD
                     EELRHEVRYSRELIGELTGTPPEGFCYPYGTLDRRVLAAVRAAGYGYGCALEPGPPAGR
                     YALPRTHVSQADRGVRLWAKDLRHGLRYGRVVGAGSRAVSPSGAGGAR"
ORIGIN
        1 ggcgggcagc gccggaaagc tgaccgttcc gtccggggtg aggaggaccg gcggcggatg
       61 acccgcccgc gccatcgtgc agcagcgcgt cgtcggatcg tagacggcgt acaggcaggt
      121 cgcgcccagc accgtcgtgc cgccccggtc ggctcgctcg cccgacgtct cctcctcgct
      181 cagccgcagg acgaggtcgt ccagccgggc cagcagctcg tcgggcggca ggtccatgtc
      241 ggccagcgtc tggacggcgg tacggagccg gcccatcgtc gccgcggcgt tgatgccgtg
      301 cccgacgaca tcgccgatga ccagaccgac ccgggcgccg gagagcggga tcacgtcgaa
      361 ccagtcgccg cccacaccgt ccttcgcgtc cgccggaagg taggaggagg ccacgtccag
      421 ggccgtcccg ccgcgcagcg tgcgcggcag caggctctgc tggaggacga gcgccgcggt
      481 gtgctcacgc gtgtagcggc gggcgttgtc cacgcacacc gcggcccgcg ccaccagttc
      541 ccgggccggc gccacgtcgt cctcgtcgaa cggcacgggg ttgcgcgacc gcatgaacgt
      601 ggtgagcccg aggacggtct cgcgcgcccg catgggcacg gagatgagcg agtgcagtcc
      661 gaactcccgg atccgcgagg cccgtacggg ctgttccacg gcccatggag gactgtcggg
      721 atcgagcatc ggcagaagga ccgggtcgcc gtcgacgagg aagcgctcgt cgtgcgacgg
      781 caggacgaag tcgacggtct cgccgatccg tacagccgcc tccgggcatc cctcgcgcac
      841 cgagcgcatc gccgtgcggc gcagcagcag ccggccgccg ttctccccgc cggacatcag
      901 cagcccggcc cggtcgtcgc cgagcgcggt gtccagcagg tcgacgtaga cgtagtcggc
      961 gaaccggggc accgcgaagt cggcgagctc ctgtgcggtg cgctgcacgt ccagagtgct
     1021 gccgatgctc gcgcccgcct cgttgaccag cgcgagcagc cggcgcgcgt tccaccggtc
     1081 ggtcacgtcg aggaccatgt agccgatgcc gatccggttc tcctcggcgt cggccagcgg
     1141 gaagaacgaa ctcgaatacg cccgccggcg atgtggatcc gaccagctcc accccaggta
     1201 ctcgtagtcc gtgatcggcg cgccggtcac caggacctgc cgcatcagcc gctcggtggt
     1261 cgccgccagc tccccgggca gcacctcgtt cgggcggcgg cccagccgct gctcccgggg
     1321 cactccgccg aaccgctcca gggtgtcgtt gagccagagg tagcgcagct gcggatccag
     1381 caccgccatc cccaccgggg acctggtcag gaagccgtcc agcaccgagc cccgcacggt
     1441 ccagcgccgg gtgcgctccc gccccgagac caggaagccc tcacgcccgg cgaccaggaa
     1501 cgagccggac acccgcaggt cggcatcgac cggccggccg tcccgcgcgc gcagcgcgac
     1561 ctgcccgctc cagccggccc cgccgcggca gtggtccgcc accgccgccg tacgacggag
     1621 gtccggcggc ccgcccagga gcagcgcgga ggaccggccg acgacctcgg cggccgggta
     1681 tccgagcagc gcctccgccc cgcgggtcca gcccatgacc gtgccgtccc cggcgatgac
     1741 cgccgcggcg tcgtcggcca cctgcgccgt cccccaggcg tccacggagg acaccgggcc
     1801 gggccccgcc ggggggccct catcggcgga tgccatgcat ctcaccacca tctctaggtt
     1861 tccatggtcg ctcctcgccg ggcggtgcgc accggtgcgt actccggacg gggcggggac
     1921 ggggccgttc ccccgttcgg cggcgtgacg gagcggttcg tgcccccgtt gggaggagag
     1981 acgagcggtc gctcgtcccc ctgccccgcg ctggacccgt gtgggaggac ggctccggct
     2041 cccgaagcgg gagccggagc cgctttcgtc cgccggatca gtagcggaag cagatcctgc
     2101 gttccctgag ccaccgcccg agcaggtctg cgaacggaac cgtcacgcac cacctgcgcg
     2161 gaggcgtcgc cggtgcggga ggagccgagg agggcgcggg ccgcaccggg ggcgagggcc
     2221 gcggggaggg ctccgaggcg cccggcgtcc cggacgggcc gggcgcgggg gccgtcggcg
     2281 cgggaggcgg ggtgggcgcg gggctcggag tgccggtagc gggccgggcg ggcgcggcca
     2341 tctccgtgag catggtccgg tagacccgcg aggaccgcgg attgcgcttg cactgccaga
     2401 cgccgtgcgg acagtagtcc gtgatcgtct ggtacagcgg atcgtgccgg tcgatccatt
     2461 cgagcatccg ccgcatgtac acggggttgt cgccgttgcg gaagaggccc cattccggga
     2521 acgagatcgg cttcccgtgg gccgccgcga agtcgacgtg cttctggagc ccgtacgggt
     2581 cgtggatctg gtcgtcgaac gactcgcccg gaggctggtc gtaggagtcc atgccgatga
     2641 tgtcgacgac gtcgtcaccg ggatagcact ccgtccaggg caccgcgtcc cgtccccggc
     2701 tcggcgtgaa gtcgaagcgg aaccgctggc ccggcacgtc ccgcatcgcc gtgacgatcc
     2761 gctgccagta cgccttccag gccgcgggat cggggccgca gcggtgggtg taggtggtcc
     2821 cgttcatctc ccagccgagc acgacgaccg tgtcgggcac gccgagcgac accagccgct
     2881 cggccagcct ggtgaagtgc cggtcgaagt cgcctgccgc gcccgaccgc agcagggagc
     2941 ggacctccgc gtccgggacg cgctcctcgt tgcgctccag catcggtacg ttcaggacga
     3001 acagccggtc ctcgtcctcg gcgcgccagt ccgcccagga cgtcaggaaa ccgggggcgc
     3061 cctcgatgtt gacccacagg tcgcccggga ggtaggtgtg ccccacgcgc aggtccgcac
     3121 cgcccagcca ccgcgacagt tccgccatcc tgctcactcc ggcgggtccg tagtcgaggt
     3181 aggcgccgtg ggcggtggcg cccgggacgg ccggcgggcc gggcgcggcg ctgtcgacgg
     3241 cgtcggcgcc cggaggcggt tggcccgcga tgccgccgcc cgccaggagg ctcagagcga
     3301 ccgccgcgac gcacgtgctg atgagtcggc gcttggccgg catggctcct cctggctgcg
     3361 gtggcgattt ccggcggttt cggcccggtg gggagagggg ttcatcatcg tgtaccgccc
     3421 tcgaacgcgg tgggccatca ctcgtgtgcg gcgttgcggg cgtgccgttc aggtgaacgc
     3481 ggggccgaac gtgagcggca gcgaaccgac gcggttgtcg aagttcgcat cgatgagccg
     3541 cggatctccc ggacggggga cgacgcgccc gcgcgtgagg atctcccgga agagggctgt
     3601 catctccagg cgggccaggt gggcgccgag gcagtggtgg gggccgccgc ccccgtaccc
     3661 caggtgcgga ttcggcgacc gggtgatgtc gaagatatcc gggcgcgtga agaccgcctc
     3721 gtcgcggttg gccgacgcgt agacgagcgc gaccctctcg ccgggccgga acgtgcgacc
     3781 gcccagccgg aattccgcca cgacggtcct gcggaactgg atgatcggcg tcgaatggcg
     3841 gacgatctcg tcgaccgcgc cgccgatgtg ccggtcgaag tcggagcgca gcagcgcctg
     3901 ttgctccgga tgccgatgga gcagcgacag cccgtgggcg agggcgttgc gcgtggtctc
     3961 cacgccggcc accagcagca gcgagaagaa cgcgccgagc cggcgcgcgg agagcgcctc
     4021 gccgtcgacg tccgcgcaca ccagggccga gacgagatcg tcctgcggac ggcggcggcg
     4081 ctcgtgcgcc agtcgcgcca tgaggagccg catccgggcc agggaggcga gcccgcgccc
     4141 cggaatccgg attctcgccc ggccgcgccg cgccacgccg acgtgctccg aggcgtggtc
     4201 gatctgcgcg gcgatccgcg cccggtacgc gccggggatg cccatcaggt cgcagatcac
     4261 ctccagcggc agccgcgagg cggccgacgg catgaagtcg ccggaacggg cctggaccat
     4321 ctcgtcgacc agcctgccgg cgagccggcc gatgttctcc tcggccgccg ccagcagccg
     4381 aggcgtgaag cgccgggaga cgatccggcg cagcgccgcg tgctcgacgc cgtccatgct
     4441 caccatcgag tccccgaaca gcgccctggc ccagcgggcg ggctccggcg tcgtgacccc
     4501 cggggcactg gcgaacacct gcggtgtgcg gctcgccgtc cgcacgtccg cgtggcgcac
     4561 cagggcgtag aagggtttgc ccgacgtgcg ggcggcgccc ggtcccgggg tgaacaggac
     4621 gggttcgtcc agctcccgca gccgggcgaa cgccctgagg cggtcggggc gcggcagccg
     4681 ccagaagtcc gggtcggcga gatcgacgtc cgccgcgtgg cgtacgcggt tcccggacgt
     4741 ccggtgcgca acggtcatga tcgacacctc cacgggttcg acggccgggc tccggccctc
     4801 agccatgttc cggtccgggg cgcgcaccgc gcctcccgac gcggccgacc cgggcggatg
     4861 gccgcgcatg tcacgcgttt gcccgaagcg ggagggccga acgccgcacg cggagaactg
     4921 ttccccgctc gcctcgttga cgacatcgcc gtcaacggcc gccacccgca aaccagtcga
     4981 cgagaggcag gcagcccccc atgccccctg cgccccccac caactccccg accgctccga
     5041 attccgccgg ttccccgacc cccgcgacgg accgggccga cgcacccccc gcgacgagcg
     5101 ggacgtcccg ccgcgtcgcc ctccgcaccg ccttcaccgc cgccgcggtc gccggcgccg
     5161 ccgcggtcct cctgccggtc ctcgaccggg aggcgggcag cgccacccgt tccgcgccgg
     5221 gaccggccgc ggaccggccg gaccgcggcc gggcgacgga gcggttcgcc gagacgtacc
     5281 ggggccgcga gatccggggc acggccaccg acgtcgtgcc ggccggccgc ggcctcccgt
     5341 ccggacaggt cacggaagtc ctcatcgacg gcaggccgct gcacgtcatg agacgggcgg
     5401 acggcaccta tctgagcgag gtgaggcact acgagtcgta cccgacgctc ctggagacgg
     5461 cgcgcgcggc cgtcgacgag ctcggctccg cccgcctcag ggccccgtcg cactccatgt
     5521 gaaaccagga aggaccgcac ccgtgcacac ccgcaggaac cagcgcgatc tcacgcgcac
     5581 cgagaagcgc cggctcgtcg acgcgatcct caggctcaag cgctccggcc gctacgacga
     5641 cttcgtcgtc atgcaccgcg agttctacgt gatggacacc gagggccagc cgcgtcccgc
     5701 ccatatgacg ccctcgttct tcccctggca ccgccgatac ctgctggagt tcgagaaagc
     5761 cctgcaagcc gtggatccga aggtcagcgt cccgtactgg gactggaccc gggacaacac
     5821 gcccgccgcc tccctgtggg cggaggactt cctcggcggc aacggcaggg ccggcgaccg
     5881 ggaggtcacc accgggccgt tcgcctaccg cgcgggcaac tggaaggtgg gggtcggagt
     5941 caccgacgag aactacctca cgcgcaactt cggccggccc gaggacccgg tggccctgcc
     6001 cacgaaggag gacgtggcgc gcgccctgaa ggacccggtc tacgacacgg agccgtggaa
     6061 cagcaccagc gtcaagggct tccgcaaccg cgtcgaggga tgggggatcc accaggtgcg
     6121 cggggtggcc aaccacaacc gggtccacca gtgggtcggc ggcccgatgg ccggagcggc
     6181 ctcgccggag gatccggtgt tctggctgca ccactccttc atggacctgc tctgggaccg
     6241 ctggcagaag gcgcacccgc gctccgccta tctgccgggc cgcgcaccgg ccgagcccgg
     6301 acccggccgg aagaccgtct tcggcctcca cgaccccatg ccgccctgga acgtgacgcc
     6361 cgcgaagctg accggccacg gcaagctcta ccgctacgcc tgacccggcg gcgacgagaa
     6421 cggccggtct cctccgcgcg ggaggagacc ggccgttctc gtcgccgccg tacgccgcct
     6481 tcctcagcgg ccgaggaccg gaagcccggc gagccggtcg cgccagtccc gggcggcggg
     6541 gaaccgtgac ttcaccgtcc cggccgcccg ctcgcggacc gccacctgcg actcgcgcag
     6601 ccgcagcagc ggctccagcc ccggccgggc cagcagcagc cgctgattga ccacccgcac
     6661 cggccgccag tggtccttgt actgctccgc gccgcgcagc aggctcagca cctcatggcc
     6721 ggcgtccgac gcctgctggg ccacctcccg cagcagcagg gtcgacacat cgaccttccg
     6781 ctcccgcagc cccgggtccg cgccgtacag atagccgccc gccagacgcc ccgactggag
     6841 cgtgaggttg gccaccacca ccgtgccgtc cagccggaac tcggtcatgg ccgccgtgcc
     6901 gtcccccgcc atcctcccga cggaccggat caggtggtcc gagaaccgcg tcctgaggtg
     6961 ctcggggttc accccccggt cgcgccactg aagttcgtgc aggcgcagca gcgttccgat
     7021 cgccgccgga acctcgctcg gcgggatccg gcgcgtctcg acgcccagcg cgtcgaccct
     7081 gcgcagtttc gcccgcagcc gctggccccg cgcacccgtc agcgggctcg ccagatcccc
     7141 caggggccgt gctggcagct ccatgcacgt cgagtccgtg agcctgctcc ggccgcccgc
     7201 ccaccggccg aacagcaact gggccgacgc gtcgggccgg acctcgcgca ggtcgacgac
     7261 cgcgtgccgg gccgcccgct ccagtccgcg acccagggcc ccgacggcgt acccggcctc
     7321 ctcctcgtcc aacaggatgt cgaagaagtc gctgatcgcc ccgcccatcg gaacgagcag
     7381 cggcatcggc cggtgcgcca gcatcagcgg cgccgcgccg atcagccgtc ccgaccgccg
     7441 taccagcagc acccgcaggc ggccctcctg gccgtaggag atccaccacg agtggagcca
     7501 ggcatggctc tggaacggcg tggcggtcgc gcaccggcgg tgcagcgcgt cccactcccc
     7561 ggccagcgcg ccgaactccc ggaagtcccg gcacagcgtc acggacaggc gtcccgcccg
     7621 gcgcgccctc atcgagcgag ttcccggtcg cccgcgggga cgccctggcc gccggaggcc
     7681 gtcggcccgg ggacctgggc cgagacgctc cagcccgcgc gccggggccg tacgagcagg
     7741 acgagtccgc ccagcagacc gcccgccgcc gcgcccacgg cggtgccgag cggcgccgac
     7801 ggcgagaccg gctgatccgg tgtcatcgcg tgggtgaact cgatcagctt cacgccggtg
     7861 tcctcggaga cgtgttcgct gctgacgacg acggcctcga tgaccgcgtt ggcgatgtcg
     7921 gccgcccggc gcggctgggt cgacgtgccg gtgaccgcga tcatgggcga gtcgggagac
     7981 gtctccgacc gcacccggga gcgcagttcg cgcaccggct cacccgccgc gccctgggcg
     8041 taggagaggg tcgcgtcgct ggtcaccaga cgcccgaagg actgcgcgta gcccagggcc
     8101 gccgacgggt ccgtctcctc ctgggcgacc gccatcgcgt agctggtggc ctcgtaccgc
     8161 ggcgccgtca gcaggccgta cgacagtccg gacgcggcgc cgagcagcac cagggcgggc
     8221 aggggccacc accggggcgg ccgggggaca gcggtgcgcg gacggcgtcg aaggacgtgt
     8281 cgccgttgct cgggagaatc ggtcatggca gggctctccg gttcggtggg acggtgtcga
     8341 gggcgtacgc gtacacgtcc atcaggcgcg tgctgctgcg ccggatgtcg tagtggccga
     8401 cggcgcgggg cggcggcagc cggtcgcagg agggggcgtg tatccgccgg cgcagcagcg
     8461 ccgtcagctc gtccggacac ccggtgatcc gggtcgcgcc cggggcgtgg gcggtcggca
     8521 ggtcgtcgat cgccgggcag gcgccgtgca ggacgggcag ccccgcggcg agcgcctcga
     8581 cggcggccag tccgaaggac tcctcgcgcg acaccgagac gaacgcgtcc acggcgctga
     8641 ggagttccgg caccccgggc accgggccgc gcccgtcgcc gcactcgccg aggaagcgga
     8701 tccggttcgc cgcgcccagc cgcccggcca gcgcccgcag ccgcccggcc tccggcccgt
     8761 cgcccgccag cagcaaccgt gcgccgggca gcgcggcgac cgcgcggacg aggacgtcga
     8821 accgtttgcc gggcaccagc cggcccactc cgccgaccac gaagacgccg tcgggaagcc
     8881 ccagggccgc acggacggcc gcgcgccgct ccggctcgaa ccggaaccgg tccgcgtcga
     8941 tgccgttcgg caccagccgg atccggtcgg ccggcacccc ccagtcccgc agccggcccg
     9001 cgacggtggg ggagacggcc acggtggccg agccgagccg ttcggtgccg aggtagaggg
     9061 cccggacgcc ccgggtgagc ggacggccct cgatctcgtc gcggcccagg gagtgctcgg
     9121 tcgccaccgt cgcgcggacc ccggcgagcc gggccgcgat cctgccgtac acgcaggccc
     9181 ggtagaggtg cgtgtgcacc aggtcgtagc ggccgtcgcg gatcagccgg accagcctcc
     9241 ccacggccga gaggtcgcgg ttgccgccca tgcccaggtg gacgacgcgg actccgtccg
     9301 cccgcagccc cgccgccacc gcccccggat tggtcagcgt gacgacgtcg cacggcaccg
     9361 gcatatgacg cagcagcagc cgcaactgct gctccgcgcc gccgaccccg agcccggtga
     9421 tgacgtggag cgccctcacc gcgcaccgcc cgcacccgat ggggagacgg cccgcgatcc
     9481 cgcgcctacg acccgtccgt accgcagccc gtgccgcagg tccttcgccc acagccgcac
     9541 cccgcggtcc gcctggctga cgtgcgtacg cggcagcgcg taccggccgg ccggcgggcc
     9601 gggctccagc gcgcagccgt agccgtaccc ggccgcgcgg acggccgcga ggacccgccg
     9661 gtccagcgtg ccgtacgggt agcagaagcc ctccggcggt gtgccggtga gctccccgat
     9721 caactcccgg ctgtaccgca cctcgtgccg cagctcctcg tccgacagag ccgtcaggcg
     9781 gcggtgcagc agcccgtgcg aaccgacctc catgcccgcc ccggcgaccc gccggatctc
     9841 ctcctcggtg agcagcggct tgcgcgggcc gagcgggtcc cagtcgttca cgcctcccgg
     9901 gcggcccggc aggacgaaga ccgtcgccgt gcagccgtgc tcgcgcagga ccggcagggc
     9961 ctccccgagg aagtccgcgt acccgtcgtc gaacgtcagc ccgaccagac cgcgccgccc
    10021 cgccgcctcc gcccgcagca gctccgccac gccgacaccg gtgagcttcc ggctgcgcag
    10081 ccacgacaac tgctgaccga gccgttcggc ggagacggtg atcccgtacg ggtcgtcccg
    10141 gggatcggcg accgagtggt aggtgaggat ccacgggcgt ctgcgggtgt gcgggcgcgg
    10201 ccggggcggg gcagtgccgg agccgggtgc cgagggatca gcggccatcg aggaacctcc
    10261 gtcggatgag tgccagcagg gatgcgaggt cgggcgagcg cagcgcgagc ccggtcagga
    10321 agaacaccgc ggggacgagg gcgcacccgg cggccaggct cagcaccgga tcggacacca
    10381 gcggagccgc ggcccagccc gccgcaccgg ccgccagcgc cgcgcccgac agccgcccca
    10441 ggccgagcac gacgtcccgg gcgcggatgg ggaccacccg ggtgccgagc cccatcagca
    10501 gcaggagcgc cgccgagctg at
//
