LOCUS       NZ_ML123034            10798 bp    DNA     linear   CON 23-MAY-2024
DEFINITION  Streptomyces sp. ADI96-02 scaffold001, whole genome shotgun
            sequence.
ACCESSION   NZ_ML123034
VERSION     NZ_ML123034.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. ADI96-02
  ORGANISM  Streptomyces sp. ADI96-02
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 6907715)
  AUTHORS   Guerrero-Garzon,J.F., Zehl,M., Urban,E., Schneider,O., Ruckert,C.,
            Busche,T., Kalinowski,J. and Zotchev,S.B.
  TITLE     Streptomyces spp. from the marine sponge Antho dichothoma: analyses
            of secondary metabolite biosynthesis gene clusters and some of their
            products
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6907715)
  AUTHORS   Guerrero-Garzon,J.F., Zehl,M., Urban,E., Schneider,O., Ruckert,C.,
            Busche,T., Kalinowski,J. and Zotchev,S.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2018) CeBiTec, Bielefeld University, Sequenz 1,
            Bielefeld 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64.8x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003846185.1-RS_2024_05_23
            Annotation Date                   :: 05/23/2024 03:48:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,089
            CDSs (total)                      :: 6,032
            Genes (coding)                    :: 5,843
            CDSs (with protein)               :: 5,843
            Genes (RNA)                       :: 57
            tRNAs                             :: 54
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 189
            CDSs (without protein)            :: 189
            Pseudo Genes (ambiguous residues) :: 0 of 189
            Pseudo Genes (frameshifted)       :: 68 of 189
            Pseudo Genes (incomplete)         :: 155 of 189
            Pseudo Genes (internal stop)      :: 10 of 189
            Pseudo Genes (multiple problems)  :: 42 of 189
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:00:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6266514
            Orig. end                         :: 6277312
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            ML123034.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Zotchev Lab, Department of
            Pharmacognosy, University of Vienna.
            Annotated using prokka v1.11 from http://www.vicbioinformatics.com.
FEATURES             Location/Qualifiers
     region          1..10798
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="25"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10798
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10798
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5798](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5798)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            269..694
                     /gene="panD"
                     /locus_tag="EES43_RS27410"
                     /old_locus_tag="EES43_27045"
     CDS             269..694
                     /EC_number="4.1.1.11"
                     /GO_function="GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="panD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964661.1"
                     /locus_tag="EES43_RS27410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_27045"
                     /product="aspartate 1-decarboxylase"
                     /protein_id="WP_124271236.1"
                     /transl_table=11
                     /translation="MLRTMFKSKIHRATVTQADLHYVGSVTIDGDLMEAADLLPGELVH
                     IVDIDNGARLETYVIEGERGSGVIGINGAAAHLVHPGDLVILISYAQVDDAEARGLVPK
                     VVHVDAGNRIVALGTDASAPVPGSRTERSPQAVVAGG"
     misc_feature    281..294
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.75"
                     /tool="antismash"
     gene            774..1127
                     /locus_tag="EES43_RS27415"
                     /old_locus_tag="EES43_27050"
     CDS             774..1127
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018513131.1"
                     /locus_tag="EES43_RS27415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_27050"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_124271237.1"
                     /transl_table=11
                     /translation="MAQNAGVEITDDREHGTLAAHDAGKVAGSIAYFVMEPAPGALVAV
                     HTVVRPEHEGRGIAGALVREFYAMAAREGVPVVPLCPYAAKWAQRHPEEAPAASAELVR
                     EAERQLGSHPELL"
     gene            complement(1673..3037)
                     /locus_tag="EES43_RS27425"
                     /old_locus_tag="EES43_27055"
     CDS             complement(1673..3037)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_Arg_deC_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_DAP_Arg_deC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1264: diaminopimelate decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378058.1"
                     /locus_tag="EES43_RS27425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_27055"
                     /product="diaminopimelate decarboxylase"
                     /protein_id="WP_124271239.1"
                     /sec_met_domain="Orn_Arg_deC_N (E-value: 2.2e-37, bitscore:
                     127.2, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="Orn_DAP_Arg_deC (E-value: 6.1e-10,
                     bitscore: 37.7, seeds: 500, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQTTSHHRERAAASSSDARFAGAVRAAVAQGLLSESHPLAGFID
                     VDGVRSSVDSLREAFAGAPEVLHTFAAKASSLVPVLRLLAACGMGCEVASPGELRLALD
                     AGFAPGSIVLDSPAKTREEIRLALALGVAVNADSLDELRRIDGLRRGDSASVIGLRVNP
                     QVGEGAIGAMSTATATSKFGVALRDPGAREAILRAFEERPWLTRLHAHVGSQGCPLELI
                     AAGVAQTYALAEEIDERLGVRQVTSLDIGGGLPVNFADDTVRPTYADYVSALTDAVPAL
                     LSGRYALVTEFGRSLLAKNGVIGARVEYTKDAGGRRVALTHAGAQTATRTVFMPQAWPL
                     RVGAFGPDGTPKDGPALAQDVAGPCCFAGDVVAHGRELPELAEGDFVVLYDTGAYYFST
                     PWSYNSLPRPAVYGFRIVAGPDGGVVFATVRETQSMESIAEESGLGQAEALTGLG"
     gene            complement(3147..4946)
                     /locus_tag="EES43_RS27430"
                     /old_locus_tag="EES43_27060"
     CDS             complement(3147..4946)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018099989.1"
                     /locus_tag="EES43_RS27430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_27060"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_124271240.1"
                     /transl_table=11
                     /translation="MGSGAPPRDPGPEATLREVLAFDEEGALRLLLAPGGVDVEVRGVS
                     FGDEAPRSRAGHLVLAVGASAGRAEAAGAVERAARSGASAVVLREAEEESGPGPAPDVL
                     AAADRSGIALLVRAAWTDWTDTAALLRSALDYHRAARTGQARGADATGEATPGTAGDVP
                     GRGGLECGGTEEAGPRGGGPGEGADPAAGGSDGPAGALAALAASIAEATGGSITIEDTR
                     FRVLAHSATRPEADGVRRSTILGGRVPEWRVAELRRSGLLRTLWTSREVIHRPADGDSP
                     ERLVIAVRSGAELLGSIWAAADGRPLSPEAGRALRRGAVVAARHLLGQRLRESGAVRRR
                     DHALRGLLYGEGDRRTHAWSLGLAPDAPCAVLAVAPVAAGPDAARTLDVLAPHARAYRP
                     SVLVLREPDRLLVLLPVPAGLPPEAREREALAPARELERVAAALPGGVPVRAGVGRVAG
                     SAAEAAEACTEAALVVRVLRDRERRARGGGAPARFASAGAVGPALDVLRMAEAVRPLWE
                     SGDGPVHRLARSDRAAGGELLRSAAAYLDAAGDVAEAARRLVLHPNTLRYRLRRLRERY
                     DVDVDDPDTRVLLTLAVRLTGDV"
     gene            complement(5001..5798)
                     /locus_tag="EES43_RS27435"
                     /old_locus_tag="EES43_27065"
     CDS             complement(5001..5798)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955648.1"
                     /locus_tag="EES43_RS27435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_27065"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_124271241.1"
                     /sec_met_domain="Linocin_M18 (E-value: 1.7e-88, bitscore:
                     294.5, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRELAPVTPAAWAEIEEEARRTFTRHVAGRRVVDVTDPAGP
                     TLAAVGDGHLRGIDPPTPDVLARARTSMPVVEWRVPFTVSREAVDDVERGSADSDWQPV
                     KDAARTCAFAEDSAIIDGYASAGILGLRDGSSHTPLPLPADARDYPVAVSQAVTRLRLA
                     GVDGPYRLLLGADAFTEAAETSDHGYPVKTHLSRLVDDDILWAPAVKGGLLLSTRGGDF
                     ELCLGQDLSIGYTDHDATSVRLYFQQAFTFRMLTPEAVVALIA"
     gene            complement(5798..6871)
                     /locus_tag="EES43_RS27440"
                     /old_locus_tag="EES43_27070"
     CDS             complement(5798..6871)
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007456253.1"
                     /locus_tag="EES43_RS27440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_27070"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_124271242.1"
                     /transl_table=11
                     /translation="MPDAVPDPVAVQPVVAPLTSAALFLVGTIEPGGERTVREVLPELA
                     AVARSIGFRYPDSGLACVTGFGSAAWDRLFAGPRPAHLHPFPELSGPLHHAPATPGDLL
                     LHIRAERMDVCYEWAAQLLRKLGGALRIVDETQGFRYLDHRDLLGFVDGTENPVGDDAR
                     AAALVGAEDPDFDGGSYVVVQKYLHDLDAWNALPVEEQERVIGRTKFDDVELPDEAKPS
                     DSHVALNTITDPDGTERDILRANMPFGSFGRGEFGTYFIGYAADPDVTERMLRNMFLGD
                     PPGSHDRILDFSTAITGTLFFAPRAAFLDAPPPPPAPPGAATRPKPVLAPVGPASAGTG
                     SDDHGSLRIGSLQESAQ"
     gene            complement(7058..8500)
                     /gene="gndA"
                     /locus_tag="EES43_RS27445"
                     /old_locus_tag="EES43_27075"
     CDS             complement(7058..8500)
                     /EC_number="1.1.1.44"
                     /GO_function="GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /GO_process="GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="gndA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1100: 3-hydroxyisobutyrate dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015612845.1"
                     /locus_tag="EES43_RS27445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_27075"
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="WP_124271243.1"
                     /transl_table=11
                     /translation="MSGTAQIGVTGLAVMGRNLARNFARNGFTVAVHNRTASKTDALVG
                     EFGGEGAFVAAHTPEEFVAALERPRRLVVMVKAGDPTDAVIQEFAPLLEEGDVIIDGGN
                     AHFEDTRRREKELRERGVHFVGAGISGGEEGALHGPSIMPGGSAESYASLGPLLERIAA
                     KAKDGTPCTAHIGPDGAGHFVKMVHNGIEYADMQLIAEAYDLLRRVAGYSPARIAETFR
                     SWNTGRLDSYLIEITAEVLAHTDADTGEPFVDIVQDRAEQKGTGRWTVQIALDLGVPVS
                     GIAEAVFARSLSGHADLREAARGLPGPSAKPLEGAQAAAFADRVEQALYASKIVSYTQG
                     FHQIRVGSDTYGWDVDLGSVAAIWRAGCIIRAAFLDRIRAAYASRPDLLSLLSDEEFER
                     EIGAAQDDWRAVVAESARQGVPTPGFAAALAYYDGLRADRLPAALTQGQRDFFGAHTYR
                     RTDRKGIFHTLWGGDRSEVTGG"
     gene            complement(8600..9076)
                     /locus_tag="EES43_RS27450"
                     /old_locus_tag="EES43_27080"
     CDS             complement(8600..9076)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006129107.1"
                     /locus_tag="EES43_RS27450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_27080"
                     /product="DUF5709 domain-containing protein"
                     /protein_id="WP_124271244.1"
                     /transl_table=11
                     /translation="MSDTEWGDEVYQPQEPPGSDFDEQLDTGDTLEDSGVDDPLDEGYS
                     PPERPWAVEDRGTTAAEQRTGESLESRLTRELPEVAEWPGDGVGDLAGGEGEPWDDEVG
                     VDRAGRLTQQADGRVPAAATADDVGVDGAAASAEEAAVHVVPDDDAPPAAEALP"
     misc_feature    9094..9109
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.54"
                     /tool="antismash"
ORIGIN
        1 cgccggcgtg gccgccgtcc tcacgaggag cagccgctca cggacatgcg gtcgccatcg
       61 aggtggaggg cacttcgtcc tacgggtccg gcttcgccca gaccgcccgg tcgcacgggc
      121 accaggtcgt cgaggtcgac cgccccgtca gcgggagtca tgaaaaacct ggggaacggt
      181 gccctgacat gcgcctcgtt gtagggtgag gacaacgccc ttgacctgca aaaacgcagg
      241 cagggagcct atgtccagga gtgccttcat gttgcgcacc atgttcaaat ccaagatcca
      301 ccgggccacc gtcacccagg ccgacctgca ctacgtcggt tccgtgacga tcgacggcga
      361 cctgatggag gccgccgacc tgctgcccgg cgaattggtc catatcgttg acatcgacaa
      421 cggcgcgcgg ctggagacgt acgtcatcga gggcgagcgc ggctccggcg tcatcgggat
      481 caacggggcc gccgcccacc tggtgcaccc cggggatctg gtgatcctca tcagctacgc
      541 gcaggtcgac gatgcggagg cgcgcggtct cgtcccgaag gtcgtccacg tcgacgccgg
      601 caaccggatc gtggcgctcg gcacggacgc ctccgcgcct gtccccggtt cccgtaccga
      661 gcggagcccg caggccgtcg tcgcgggcgg ctgacaccgc cgcctgccca cgccccgccc
      721 ccccggccac ggaaacgcgg ccgggggcac cacgaacgag gagagcagga accatggcgc
      781 agaacgccgg tgtggagatc acggacgacc gggagcacgg caccctcgcg gcgcacgacg
      841 cgggcaaggt cgccggaagc atcgcgtact tcgtcatgga acccgcgccc ggggcgctcg
      901 tggccgtgca caccgtcgtc cggcccgaac acgagggcag gggcatcgcc ggtgcgctgg
      961 tccgcgagtt ctacgccatg gccgcccgcg agggcgtccc cgtcgtgccg ctgtgcccgt
     1021 acgcggcgaa gtgggcgcag cgccatcccg aggaggcccc cgccgcgtcg gccgaactgg
     1081 tccgggaggc ggagcggcag ctggggagcc accccgagct gctctgagtc cgggccggtc
     1141 gatggcgcgg tgcgccacac gacgggcacg agtggatcac gtgtggagtc ggcaggcgcg
     1201 ggcagccgca ccgggagacg gtggaggaga gctccgcccg tccgtttcgt accgctgtgg
     1261 aggatcgtga tgacccaccc gtaccccgac cccgtaccgc cctcgccgac gcctccgccc
     1321 gttcccgggc ctcccactcc ggtgcccggt ccgcctcccg tcccgccggg gcccgggcct
     1381 gtgccgcccg gtcccgcgcc ggacccgatt ccgccgtcgc ccggcccgga ccccacgccc
     1441 gacccggagc cccagcccgc cggctgaacc cggccgggcc cgagccggcc caagccggtg
     1501 aggcgagaag cgtggattcc gctcaggcac cgacgggcgc cggccccctg ccgggccggc
     1561 gcccgccccc tccacgtccg gccgaagccg ggcgtatgac gggaacccgg cgtgcgatgg
     1621 gaacctggcg gcagccggcc ccacgaccgg catcggcccg gaaccggggg cgtcagccca
     1681 gaccggtcag cgcctccgcc tgcccgaggc cgctctcctc cgcgatcgac tccatcgact
     1741 gcgtctcgcg caccgtcgcg aacaccaccc cgccgtccgg ccccgccacg atccggaacc
     1801 cgtacaccgc gggccgcggc aggctgttgt aggaccacgg agtggagaag tagtacgccc
     1861 ccgtgtcgta cagcacgacg aagtcccctt cggccagctc cggaagctcc ctgccgtgcg
     1921 ccaccacgtc tcccgcgaag cagcacggcc ccgcgacgtc ctgcgccagt gccggcccgt
     1981 ccttcggtgt tccgtccggg ccgaacgcac cgacgcgcaa cggccacgcc tgaggcatga
     2041 agacggtacg ggtggcggtc tgcgcgcccg cgtgggtgag ggcgacgcgc cgtccgcccg
     2101 cgtccttcgt gtactccacc cgcgccccga tgaccccgtt cttcgccagc agtgaccggc
     2161 cgaactcggt gaccagggcg taccggccgg acaggagcgc gggaacggcg tcggtcaggg
     2221 cgctgacgta gtcggcgtag gtcgggcgca cggtgtcgtc ggcgaagttg acgggcagcc
     2281 cgccgccgat gtccaggctg gtcacctgcc ggacgccgag gcgttcgtcg atctcctcgg
     2341 ccagcgcgta cgtctgcgcc acccctgccg cgatcagctc caacgggcag ccctgggacc
     2401 cgacatgcgc gtgcagccgg gtgagccacg ggcgctcctc gaaggcccgc aggatcgcct
     2461 cgcgggcccc ggggtcgcgc agcgccacgc cgaacttcga cgtcgcggtc gccgtgctca
     2521 tcgccccgat cgcaccctcg cccacctgcg ggttgacgcg cagtccgatc acggaggcgc
     2581 tgtcgccgcg ccgcagtccg tcgatccggc gcagctcgtc cagactgtcg gcgttgacgg
     2641 cgaccccgag ggccagcgcc agccggatct cctcgcgggt cttggccggg gagtccagga
     2701 cgatcgaccc cggcgcgaac ccggcgtcca gggcgagccg cagctcaccc gggctcgcca
     2761 cctcgcaccc catgccgcat gcggcgagca gccgcagcac cggaacgagg gacgaggcct
     2821 tggccgcgaa cgtgtgcagg acctcgggcg cgcccgcgaa cgcctcgcgc agggagtcga
     2881 cggacgagcg caccccgtcc acgtcgatga acccggccag cgggtgggac tcgctcagca
     2941 gcccctgcgc cacggccgcc cggacggccc cggcgaaccg ggcgtcggac gacgaggcgg
     3001 cggcccgctc acggtgatgg gaagtcgtct gcttcatgat gcgaggatcg gtgatggctg
     3061 ccccacaggt cttcagcggc acggacaaca ctctccgcat tccttggccg agcggacaac
     3121 ccgcgccgcg aaaagggccg gaccgctcaa acgtcacccg tgaggcggac ggccagcgtc
     3181 agcagcaccc gtgtgtccgg gtcgtccacg tcgacgtcgt accgctcccg cagccgccgc
     3241 agccggtagc ggagggtgtt cgggtgcagg accagccggc gtgccgcctc cgccacgtcg
     3301 cccgcggcgt ccagatacgc ggcggccgac cgcagcagtt cgccgcccgc cgcccggtcg
     3361 gaccgcgcca gccggtgcac cggcccgtcg ccggactccc acaacggccg taccgcctcc
     3421 gccatccgca gcacgtccag cgccggcccc accgcacccg ccgacgcgaa ccgcgccggc
     3481 gcgccaccgc cccgcgcccg ccgctcgcgg tcccgcagca cccgcacgac cagggcggcc
     3541 tccgtgcacg cctcggccgc ctccgccgcc gatccggcca cccggcccac cccggcccgt
     3601 accggaactc cgccgggcag cgccgccgcc acccgctcca gctcccgggc cggcgcgagc
     3661 gcctcccgct cacgcgcctc gggcggcagc ccggcgggaa cggggagcag gaccaggagg
     3721 cgatccggtt cgcgcaggac cagtaccgag ggccggtacg cccgtgcgtg cggggcgaga
     3781 acgtccaggg tccgcgccgc gtccggcccg gcggcgaccg gcgcgaccgc cagcaccgcg
     3841 cagggcgcgt cgggcgccag ccccagcgac caggcgtgcg tgcggcggtc cccctctccg
     3901 tacaggagtc cgcgcagcgc gtggtcgcgg cggcgcaccg caccgctctc ccgcagccgc
     3961 tgcccgagca ggtgccgcgc cgccaccacc gcaccgcgcc gcagcgcccg gccggcctcc
     4021 ggcgagaggg gcctgccgtc cgccgccgcc cagatggagc ccagcagctc ggctccgctg
     4081 cgtacggcga tgaccagccg ctccggactg tcgccgtccg ccgggcggtg gatcacctcc
     4141 cgtgacgtcc acagcgtccg cagcagcccg ctgcgccgca gttcggcgac gcgccactcg
     4201 gggacgcgac cgcccaggat ggtggacctg cgcaccccgt cggcctcggg ccgggtggcc
     4261 gagtgcgcca gtacgcggaa acgggtgtcc tcgatggtga tggagccgcc cgtcgcctcg
     4321 gcgatcgacg cggccagcgc ggccagcgcg cccgccgggc cgtcgctccc gccggccgcc
     4381 ggatccgccc cctcgccggg cccgccgccc ctcggcccgg cctcctccgt cccgccgcat
     4441 tccagcccgc cgcgtcccgg cacgtcgcct gccgtgccgg gtgtcgcctc gcctgtcgcg
     4501 tccgcgcccc gcgcctgacc ggttctcgcc gcccggtggt agtccagcgc cgaacgaagc
     4561 agcgccgcgg tgtcggtcca gtccgtccag gccgcccgta ccagcagtgc gatgccggac
     4621 cggtccgccg cggcgagcac gtccggggcc ggaccgggac cggattcctc ctccgcctcc
     4681 cgcagcacca cggcgctcgc gcccgagcgc gccgcccgct ccaccgcccc ggccgcctcg
     4741 gcccgtccgg cggacgcccc gacggcgagg acgaggtgcc cggcgcggct ccgcggcgcc
     4801 tcgtcgccga acgacacgcc ccggacctcc acgtccactc cgccgggcgc cagcagcagg
     4861 cgcagcgcgc cctcctcgtc gaacgccaga acctcccgga gcgtggcctc cggccccgga
     4921 tcccgcggcg gtgctccact gcccatgact cccgagcata cgagcgtcga cggcgggtgc
     4981 gcggggcacg gggcacgggc tcaggcgatc agggcgacca cggcctccgg cgtcagcatc
     5041 cggaaggtga aggcctgctg gaagtagagc cggacgctgg tcgcgtcgtg gtcggtgtag
     5101 ccgatcgaca ggtcctggcc caggcacaac tcgaagtcgc cgccccgggt ggacaggagc
     5161 aacccgccct tcaccgcggg cgcccacagg atgtcgtcgt ccacgaggcg gctcaggtgc
     5221 gtcttgaccg gatagccgtg gtcggaggtc tcggccgcct cggtgaaggc gtccgcgccg
     5281 agcagcagcc ggtacggacc gtcgacgccg gccagccgca gccgcgtcac cgcctgactg
     5341 accgccaccg ggtagtcccg cgcgtcggcg ggcagcggca gcggcgtgtg cgaggagccg
     5401 tcgcgcagcc cgaggatccc ggcggacgcg tagccgtcga tgatcgccga gtcctccgcg
     5461 aacgcacagg tgcgcgccgc gtccttgacg ggctgccagt cgctgtcggc ggagccgcgt
     5521 tccacgtcgt ccacggcctc ccggctcacc gtgaagggca cacgccactc gacgaccggc
     5581 atcgacgtgc gggcgcgggc cagcacgtcc ggggtgggcg ggtcgatgcc gcgcagatgg
     5641 ccgtcgccga cggcggccag cgtcggaccc gccggatcgg tcacgtcgac gacacgccgg
     5701 ccggccacat ggcgggtgaa ggtgcgccgc gcctcctcct cgatctccgc ccaggcggcg
     5761 ggggtgacgg gcgcgagttc gcggtgcaga ttgttcatca ctgggcgctt tcttgcaggc
     5821 tgccgatgcg cagcgaaccg tggtcgtccg atccggtccc ggcggacgcc ggccccaccg
     5881 gtgcgagcac cggttttggc cgggtcgcgg ccccgggcgg ggcgggcggg ggcgggggag
     5941 cgtcgaggaa ggcggcgcgg ggggcgaaga acagggtgcc ggtgatcgcg gtggagaagt
     6001 cgaggatgcg gtcgtgcgaa ccgggcggat cgccgaggaa catgttgcgg agcatccgtt
     6061 cggtcacgtc gggatcggcc gcgtagccga tgaagtacgt cccgaactcc ccccggccga
     6121 agctcccgaa cggcatgttc gcccgcagga tgtcgcgctc ggtcccgtcc ggatcggtga
     6181 tcgtgttgag cgcgacgtgc gagtcggagg gcttggcctc gtcgggcagc tccacgtcgt
     6241 cgaacttggt gcggccgatg acccgttcct gctcctccac gggcagggcg ttccaggcgt
     6301 ccaggtcgtg caggtacttc tgcacgacca cgtagctccc gccgtcgaag tccgggtcct
     6361 ccgcgccgac gagcgccgcc gcgcgggcgt cgtcgcccac cgggttctcc gtaccgtcca
     6421 cgaaaccgag caggtcccgg tggtcgaggt agcggaaccc ctgcgtctcg tccacgatcc
     6481 gcagcgcccc gccgagcttc cgcaggagct gggcggccca ctcgtagcag acgtccatcc
     6541 gctcggcccg gatgtgcagc agcaggtcgc cgggggtcgc gggcgcgtgg tggagcggcc
     6601 cgctcagctc cgggaacggg tgcaggtgcg cggggcgcgg tccggcgaag agccggtccc
     6661 aggcggcgga cccgaagccg gtcacgcacg ccaggccgga gtccggatac cggaagccga
     6721 tggagcgggc gacggccgcc agctccggca gcacctcgcg caccgtgcgc tcgccgcccg
     6781 gttcgatcgt gccgaccagg aacagggcgg cgctggtcag cggggcgacg acgggctgga
     6841 ccgcgaccgg gtccgggacg gcatcgggca tgcgcgcact cctgggacgg gtgacgacgg
     6901 gcggacggtg tacggccggg cggttgccgg tcgcacggta gggcgggcgg gacggccggg
     6961 gtgcggagcc acgacggcac cgcagcacgg cggtccgggc ccgcaagcca cttacgcacg
     7021 ctccgggccc cgccgcaccc cgggtggccc ggtcgggtca gccgcccgtg acctcggacc
     7081 ggtccccgcc ccagagcgtg tggaagatcc ccttccggtc cgtgcgccgg taggtgtgcg
     7141 ccccgaagaa gtcccgctgc ccctgcgtca gcgccgcggg cagccggtca gcgcgcagtc
     7201 cgtcgtagta cgccagcgcc gccgcgaaac cgggcgtcgg cacgccctgg cgcgccgatt
     7261 cggcgaccac cgcccgccag tcgtcctgtg ccgcaccgat ctcccgctcg aactcctcgt
     7321 ccgacagcag gctcagcagg tcgggccgcg aggcgtaggc ggcccggatc cggtccagga
     7381 acgccgcccg gatgatgcag cccgcccgcc agatcgccgc cacggaaccg aggtccacat
     7441 cccagccgta ggtgtcgctg ccgacccgga tctggtggaa gccctgcgtg tacgagacga
     7501 tcttggaggc gtacagggcc tgctcgaccc ggtccgcgaa cgccgccgcc tgcgcgccct
     7561 ccaacggctt cgcggagggt cccggcagtc cgcgcgcggc ctcccgcagg tccgcgtggc
     7621 ccgagaggga gcgggcgaac accgcctcgg cgatccccga caccggcacc ccgaggtcca
     7681 gcgcgatctg cacggtccag cgcccggtgc ccttctgctc ggcccggtcc tggacgatgt
     7741 cgacgaacgg ctcgccggtg tccgcgtccg tatgggccag gacctcagcg gtgatctcga
     7801 tcaggtacga gtcgaggcgt ccggtgttcc agctccggaa cgtctccgcg atccgcgcgg
     7861 gagagtagcc ggccacgcgg cgcagcaggt cgtacgcctc ggcgatgagc tgcatgtccg
     7921 cgtactcgat gccgttgtgg accatcttca cgaagtgacc ggccccgtcc ggcccgatgt
     7981 gcgcggtgca cggcgtgccg tccttcgcct tcgcggcgat cctctccagc agcggaccga
     8041 gcgacgcgta ggactcggcc gagccgcccg gcatgatgct cggaccgtgc agcgcgccct
     8101 cctcgccgcc cgagatgccc gccccgacga agtggacgcc gcgctcgcgc agctccttct
     8161 cacggcggcg ggtgtcctcg aagtgggcgt tcccgccgtc gatgatgacg tcgccctcct
     8221 ccagcagcgg ggcgaactcc tggatcacgg cgtcggtggg atcaccggcc ttcaccatga
     8281 cgaccagacg ccggggcctc tccagcgcgg cgacgaactc ctcgggcgtg tgcgccgcga
     8341 cgaacgcgcc ctcgccgccg aactccccga cgagcgcgtc ggtcttggac gccgtgcggt
     8401 tgtgcacggc gacggtgaag ccgttgcggg cgaagttccg ggcgaggttg cggcccatca
     8461 ccgcgagtcc ggtgacgccg atctgggcag taccgctcat ctgagtgctc ctgcgatccg
     8521 gggtgggttc ggtcggggaa gtgccggggg gcgtacggga cgggggacgg ccgcgacggc
     8581 cgcgttcaaa cggtcgcgtt caggggaggg cctccgcggc gggcggagcg tcgtcgtcgg
     8641 ggaccacgtg cacggcggcc tcctcggccg aggccgccgc gccgtcgacg cccacgtcgt
     8701 cggcggtcgc cgcggcgggg acccggccgt cggcctgctg cgtcagccgt ccggcgcggt
     8761 cgaccccgac ctcgtcgtcc cagggctcgc cctcaccgcc ggccaggtcg cccacgccgt
     8821 cgccgggcca ctccgcgacc tcggggagtt ccctggtcag ccgggactcc agggactcgc
     8881 cggtgcgctg ctccgcggcg gtggtgcccc ggtcctcgac cgcccacggg cgttcgggcg
     8941 gcgagtagcc ctcgtcgagc gggtcgtcca ccccggagtc ctccagcgtg tccccggtgt
     9001 cgagctgctc gtcgaaatcg gagcccggcg gctcctgcgg ctggtagacc tcgtcccccc
     9061 actcggtgtc gctcatggcc cgtcctttcg tcccacgaga atcactgtcc cctgtacgca
     9121 attccactcc ggtaacgccg ctgccgcaaa gggaagatcc tcatccagtg gtcggccgag
     9181 gccgacccgc gccccgaggg cagggcgcgc acgggtgcac gagcgcccgc acgcgggccg
     9241 tggccccggg gccggcacgt acactcggcc catgggtcgt gaagccgaac cgcggaagtg
     9301 cccgcaccgg gcggcctccg cgctgcacgg acacggctag cgaccttcgg ccacggctgg
     9361 cgaccttcgg cgtccagcgt ccggggcgtc tcagcccgta gggctcgcat cggcccgttc
     9421 ctcttgcttc cgcccgcttt cagccctgtg cgcccgagcc ctttccgcgt tgctccgccc
     9481 cgcccccggg cgcgggcgcg gtgcgacccg tggcctccac cggcgccaca cgtttccgca
     9541 cgcatcacgc atcacgcatc acgcatcacg catcacgcat cacgcatcac gcatcacgca
     9601 tcacgcatca cgcggcacac cgcgcatccg cccgtgccgc cgcccggcga cggccgcacc
     9661 accggggccg ccgccgctct cgaagggacc accgtgaagc tgagcgacct cctggcaggc
     9721 caggaccatc acgtactccg gggagatccc cggcggaccg agatcaccgc cggtgccacg
     9781 ttcgacgcgg accgcgtcac ccccggctcc gtcttcgcag ccgtgccggg ccacctcacg
     9841 ggcggccccg aagccctggc gcccgccgtc gcccgcggtg cggtcgccgt cctggtcgac
     9901 gaccaggcgc cgcccctgcc cgccgcgacg ggccacgtct gcgccgtccg ggtgccggac
     9961 gtccgcaagg ccgccgccgt cgtcgcggca cgctacttcg gcgagccggg ccgcgccatg
    10021 gagctgatcg ccgtcaccgg gaccaacggc aagacctccg tgtcgtacat ggtcgagtcc
    10081 gtgctgcgga tcgccgaggg cgtccgggtc ggcgtgatcg gtacggcggg aagccggatc
    10141 ggggacgagc ccgtcccgat gccgccctcg gtgctcacca ccccggagtc gcccgatctc
    10201 cagtacctgc tgggacacat gcgcgaccgc gccaccggca gcgtcgtcct ggaggccacc
    10261 tccatgggcc tgctgacgca ccgtctggac cgtacgttca tcgacgtcgg cgtcttcacg
    10321 aacctgacgc aggaccacct cgacgaccac ggcaccatgg agaactaccg cgacgccaaa
    10381 ctccgcctct tccagggcct gtgccggcga gcggtgatca acgccgacga cccggtgggc
    10441 gccgggatcc gggcgctgat gcccgacgcg gtgaccacgt acgcgctgga cgccgacgcc
    10501 gactaccggg cgagcgacct ggtcgtggac gcctcgggaa cgcgtttcac cctgcaccac
    10561 gacgggcgca agtatccggc ggccattccg tcgcccggcc ggttctcggt ctccaacgcc
    10621 ctggccacgc tggccgcctg ccatcttctg ggccacggcc tggccgggct cgtcgacgcc
    10681 ctggagcgca tgccgcagat ccccggacgc ttcgaacgcc tccaggcccc gaacggcgcc
    10741 tcggtgatcg tcgactacgc gcactcgccg gactccctcg acaaggtgct caccacga
//
