LOCUS       NZ_ML123034            11302 bp    DNA     linear   CON 23-MAY-2024
DEFINITION  Streptomyces sp. ADI96-02 scaffold001, whole genome shotgun
            sequence.
ACCESSION   NZ_ML123034
VERSION     NZ_ML123034.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. ADI96-02
  ORGANISM  Streptomyces sp. ADI96-02
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 6907715)
  AUTHORS   Guerrero-Garzon,J.F., Zehl,M., Urban,E., Schneider,O., Ruckert,C.,
            Busche,T., Kalinowski,J. and Zotchev,S.B.
  TITLE     Streptomyces spp. from the marine sponge Antho dichothoma: analyses
            of secondary metabolite biosynthesis gene clusters and some of their
            products
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6907715)
  AUTHORS   Guerrero-Garzon,J.F., Zehl,M., Urban,E., Schneider,O., Ruckert,C.,
            Busche,T., Kalinowski,J. and Zotchev,S.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2018) CeBiTec, Bielefeld University, Sequenz 1,
            Bielefeld 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64.8x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003846185.1-RS_2024_05_23
            Annotation Date                   :: 05/23/2024 03:48:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,089
            CDSs (total)                      :: 6,032
            Genes (coding)                    :: 5,843
            CDSs (with protein)               :: 5,843
            Genes (RNA)                       :: 57
            tRNAs                             :: 54
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 189
            CDSs (without protein)            :: 189
            Pseudo Genes (ambiguous residues) :: 0 of 189
            Pseudo Genes (frameshifted)       :: 68 of 189
            Pseudo Genes (incomplete)         :: 155 of 189
            Pseudo Genes (internal stop)      :: 10 of 189
            Pseudo Genes (multiple problems)  :: 42 of 189
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:00:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5578851
            Orig. end                         :: 5590153
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            ML123034.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Zotchev Lab, Department of
            Pharmacognosy, University of Vienna.
            Annotated using prokka v1.11 from http://www.vicbioinformatics.com.
FEATURES             Location/Qualifiers
     region          1..11302
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="23"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11302
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11302
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6302](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6302)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            1278..2006
                     /gene="hemQ"
                     /locus_tag="EES43_RS24270"
                     /old_locus_tag="EES43_23885"
     CDS             1278..2006
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965515.1"
                     /locus_tag="EES43_RS24270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_23885"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_124270686.1"
                     /transl_table=11
                     /translation="MSAPETVTSSKSPNAGKKAKDLNEVIRYTLWSVFKLRDVLPLDRA
                     GYAEEVQELFDRLAAQDVTVRGTYDLSGLRADADLMIWWHAETADELQEAYNLFRRTRL
                     GRALDPVWSNMALHRPAEFNKSHIPAFLADETPRDYISVYPFVRSYDWYLLPDEDRRRM
                     LADHGKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARLHVRE
                     EVPFYTGRRKSVADLVAGLA"
     gene            complement(2180..3739)
                     /locus_tag="EES43_RS24275"
                     /old_locus_tag="EES43_23890"
     CDS             complement(2180..3739)
                     /NRPS_PKS="Domain: Abhydrolase_1 (101-471). E-value:
                     3.5e-18. Score: 58.1. Matches aSDomain:
                     nrpspksdomains_EES43_RS24275_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956907.1"
                     /locus_tag="EES43_RS24275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_23890"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_124270687.1"
                     /sec_met_domain="PF00561 (E-value: 1.6e-21, bitscore: 75.6,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTAPLYATIGSLVLSALTVTPASAWDGPPAAETRGTAVAAARAA
                     AAGIDFGACPEEEMLPETLKCGRVTVPLDYAEPDGPQIELTVSRVAATGDAAGRQGALV
                     YNPGGPGSSSITFPLLGEMSEWREVARAYDLVGYAPRGVGDSAPLSCQDPAAFAERPTD
                     APVHPTAADKEKRIARARAYALGCERNAGDALRHYTSLNNARDLDVLRAALGEPELTFM
                     GASYGTYFGALYATLFPSHVRRMVFDSAVDPDPRRIWYRSNLGQSQALESRWADFRRWV
                     AAHHDVYGLGATPEAVQRGYDEVRAEIAARPAGGTVGTGQFQAAFLDAAYYDDYWAMRA
                     TALSEYRRGNPEPLIAQASRDAVTAKGDENERAVYTAVECNDAPWPEDFAVWDRDHTAL
                     AAAAPLATWHNAFTNLPCAFWPTPRQRPLDVGTRPGELPPVLILAAERDAVTPYEGALE
                     LRRRLPGAVLVTERGAGTHGVGGAGNACVDGHLRRYLLTGRTPERDADCAARPEPNAVS
                     LD"
     aSDomain        complement(2327..3436)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EES43_RS24275_Abhydrolase_1.1"
                     /evalue="3.50E-18"
                     /label="EES43_RS24275_Abhydrolase_1.1"
                     /locus_tag="EES43_RS24275"
                     /protein_end="471"
                     /protein_start="101"
                     /score="58.1"
                     /tool="antismash"
                     /translation="ALVYNPGGPGSSSITFPLLGEMSEWREVARAYDLVGYAPRGVGDS
                     APLSCQDPAAFAERPTDAPVHPTAADKEKRIARARAYALGCERNAGDALRHYTSLNNAR
                     DLDVLRAALGEPELTFMGASYGTYFGALYATLFPSHVRRMVFDSAVDPDPRRIWYRSNL
                     GQSQALESRWADFRRWVAAHHDVYGLGATPEAVQRGYDEVRAEIAARPAGGTVGTGQFQ
                     AAFLDAAYYDDYWAMRATALSEYRRGNPEPLIAQASRDAVTAKGDENERAVYTAVECND
                     APWPEDFAVWDRDHTALAAAAPLATWHNAFTNLPCAFWPTPRQRPLDVGTRPGELPPVL
                     ILAAERDAVTPYEGALELRRRLPGAVLVTE"
     misc_feature    complement(2525..2527)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    3727..3739
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.46"
                     /tool="antismash"
     gene            complement(3961..4995)
                     /locus_tag="EES43_RS24280"
                     /old_locus_tag="EES43_23895"
     CDS             complement(3961..4995)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072528.1"
                     /locus_tag="EES43_RS24280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_23895"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_206430113.1"
                     /transl_table=11
                     /translation="MNALAALLTLAVVVSSLLLIVRLLAAKGRRPAPGPSPAVHDLYEA
                     AFLGGGPGRVVDTALTALHADGRLLIGGPGIVSVQRPQAHDPVERAVLQELGAAPSGAL
                     AALRDAVMRHPAVQEIGDGLAARGLLVPPADTRPLRRWGVVQGVCCLIAFPVSLGLTFV
                     QYAMVDGLSDMPLPFIVKVVPAILLGAISGLVVAVSSAKRITRAGRRAAEGFRAAHASV
                     ESPARLVAVRGPRALPDPGLQGHLVAAARLRGPGDRSGPIAAGSSAAGTAAVAATVWCA
                     STSPGHTSCGGSTGGGSGSGCGGGSGCGAGSGSGCGGGGSSCGGGGGSSCGGGGGSSCG
                     GGGS"
     misc_feature    complement(4997..5008)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: strong, score: 21.4"
                     /tool="antismash"
     gene            complement(5001..6302)
                     /locus_tag="EES43_RS24285"
                     /old_locus_tag="EES43_23900"
     CDS             complement(5001..6302)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014153332.1"
                     /locus_tag="EES43_RS24285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_23900"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_124272138.1"
                     /sec_met_domain="DUF692 (E-value: 5.5e-91, bitscore: 302.9,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELGIGIGWRPEIAAEVEALSGIDWVEAVAENICDDHLPNSLARL
                     RERGVTVVPHGVSLGLGGADRPDAGRLAGLAARAELLGAPLVTEHIAFVRAGGPLTASP
                     RLEAGHLLPVPRTWDALEVLCENVRIAQDALPVPLALENIAALVSWPDEELTEGRFLAE
                     LVERTGVRLLIDVANLHTNRVNRGEDPAKALDELPVEAIAYVHVAGGVEKDGVWHDTHA
                     HPVTAPVLDVLRELRSRVDPPGVLLERDDAFPPAPELAAELDTIRATLRTASTAPAPRP
                     AATRPAAVAVPAVPVAPGARERTAVAQTALLSALVAGTPAPAGFDRRRLGVQSRALAAK
                     RADVVAKVAPELPEILGDGYRAAFLAYAAGRPMSDGYRRDALAFAERLLVTGAPADPVA
                     RRRLTYWWQDRSGPRPPRRAARLVRAARAALVGK"
     gene            complement(6500..7270)
                     /locus_tag="EES43_RS24290"
                     /old_locus_tag="EES43_23905"
     CDS             complement(6500..7270)
                     /EC_number="3.1.1.29"
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018100702.1"
                     /locus_tag="EES43_RS24290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_23905"
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="WP_124270689.1"
                     /transl_table=11
                     /translation="MSSNDIPATDAPTTAPATGVGVGAPAESPFRDGTTPRDEAPQYVL
                     PLVVHLEKTDPPARTDALRTAARAVLTILCDERSLGEGEWARAMRDWQDARIRKVVRRA
                     RGAEWRKAAALPGITVTGESAEVRVFPPVPLDGWPKELAKLQVSGTDLDDPEPPAAPDP
                     AGPVLWLNPELDMSAGKTMAQAGHAAQLAWWELTDAERTAWREAGFPLAVATPGPARWR
                     ELTASGLPVVRDAGFTEIAPGSCTVVADHPALRR"
     gene            7342..8016
                     /locus_tag="EES43_RS24295"
                     /old_locus_tag="EES43_23910"
     CDS             7342..8016
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014153329.1"
                     /locus_tag="EES43_RS24295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_23910"
                     /product="AIM24 family protein"
                     /protein_id="WP_124270690.1"
                     /transl_table=11
                     /translation="MKSDLFSSEHMAQPATAPGMTLQNAKSIKYAVDGEMHARQGSMIA
                     FRGDLQFERKGQGLGGMLKRAVTGEGLALMAVRGRGEAWFAHEAANCFVVEMEQGDVLT
                     VNGRNVLCFDATLSYEIKTVKGAGMTGGGLFNSVFSGHGKLGLMCDGHPIVIPVTADQP
                     VYVDTDAVVGWSRQLSTTLHRSQSVGSMVRGGSGEAVQLMLQGEGFVIVRPSELKPEKT
                     AN"
     gene            complement(8073..8939)
                     /locus_tag="EES43_RS24300"
                     /old_locus_tag="EES43_23915"
     CDS             complement(8073..8939)
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018514525.1"
                     /locus_tag="EES43_RS24300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_23915"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_241192523.1"
                     /transl_table=11
                     /translation="MTISALTGAVAGALLSGVLVGCGSPAGPRAETAPASPSPASPASP
                     AVRKPPTMAPGPAGRVPVFERRAAAGGPAGKAADKVVALTFDADMTADQGPRAASGERF
                     DNPELIALLRRLKVPSTVFMTGRWAEEYPAQAKRIGTDPLFEIANHSYSHYAFSSPCYG
                     LPEVAGADMAGELARAFTAFRTAGARNVVPYFRFPGGCYDDAALRALAPAKVTAVQWDV
                     VSGDAFATDADAVARQVLDGVKPGSLVVMHCTRSAAPTTGEAVRTIVPELRKRGYRFVK
                     VSELMEG"
     misc_feature    8928..8943
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.31"
                     /tool="antismash"
     misc_feature    8930..8944
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.6"
                     /tool="antismash"
     misc_feature    8931..8944
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.59"
                     /tool="antismash"
     misc_feature    complement(8931..8946)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.85"
                     /tool="antismash"
     misc_feature    complement(8958..8972)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.85"
                     /tool="antismash"
     misc_feature    complement(8968..8982)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.38"
                     /tool="antismash"
     gene            complement(9061..10392)
                     /locus_tag="EES43_RS24305"
                     /old_locus_tag="EES43_23920"
     CDS             complement(9061..10392)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1074: major
                     facilitator family transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019525561.1"
                     /locus_tag="EES43_RS24305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_23920"
                     /product="MFS transporter"
                     /protein_id="WP_124270692.1"
                     /transl_table=11
                     /translation="MSTSTIAGQPSVLGTKDRVKTAIAAAVGTSVENYDFIAYGTASAL
                     YFGAVFFPESDRFVGTLLSFATLAVGFVMRPLGGAIGGYFADKLGRKPVLVAAMIVMGA
                     ATFLIGVLPTYEQVGVLAPVLLVLIRMVQGLAFGAEWGGAITMAYEHAPWHRRGMFAAI
                     PQSGNPLGIALASGVFAWSATLSGDWQWRTPFLLSAVLVIVALIVRSRLSESPEFQEAQ
                     ASGTTEKNPFLATLRDDWQSILRVIALRVVESFAYYSTATYLLSYITERHPDLRPVALG
                     AITAASILAIAVTFLAGSLTDRIGRRPLYIAACTAAILFAFPMYLLTNTAQPALVIAVF
                     VIGIGVVHASFTGVQGSLLTEQFRTATRTSGASLGYQVAAAVGGFAPLLAAALVGAFGW
                     PGAALLYLGAAIVGLVGILATKETWGRAERERVLALVAEEQRRS"
     gene            complement(10614..11069)
                     /locus_tag="EES43_RS24310"
                     /old_locus_tag="EES43_23925"
     CDS             complement(10614..11069)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019525562.1"
                     /locus_tag="EES43_RS24310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_23925"
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="WP_124270693.1"
                     /transl_table=11
                     /translation="MKIALGADHAGYPLKEAVRRTIEDLGHEVVDCGTHGSDPVDFPDI
                     TRATCDPVLSGTADRAVLVCGTGQGAVMAANKLPGIRCGLAHEPYSAHQAVEHDDANAI
                     AMGAWLVPHALVPDIVREFLAATFDDDEDTRRRVAKLNALDSLTPKT"
ORIGIN
        1 gctggcccgc aggcccgagg cggtcgccct cgcccgcgcg gtcggtctgg ccgggcgcct
       61 ccagcccccc gccaccgcca ccgcgtcgct gtggacccgc gacgccctgc gtccgatgcc
      121 caaggggcac gtcatgggcg tcccgagcga cccggccgcg ctcagcggcg tgctgtcgcc
      181 cgagggactg gcccggatcg ccgaggaacc cggcctcacc ccgacccccg tcggcgacga
      241 cgtggcggtc ggggcgtacg tcgccgaccg gttcggccgc gaggtcgtgg accggctcgt
      301 ggaaccgctc ctgggcggcg tgtacgcggg cgacgcctac cggatctcga tgcgcgccgc
      361 cgtaccgcag ctcttcgagg cggtgaagga gggcggctcg ctgctcgacg cggtgcgccg
      421 cgtccaggag cgcgcggcgg cccggcagca gaccggcccg gtcttccagg gcatcgaggg
      481 cggcatcggc accctcccgt acgccgtggc cgacgccgta cgcgccgggg gcggcgagat
      541 cctcaccgcc acccccgtcc tcggactcac ccggaccgcg acgggctggg ccgtccgcac
      601 cgacagccgg gtgatcgccg ccgacggcgt cgtcctggcc gcccccgcct ggtccgcctc
      661 caccctgctg gccgcctcct cgccggccgc ctccgccgag ctcgccggtg tcgagtacgc
      721 ctcgatggcc ctggtcaccc tcgcctaccg ccgcgccgac gtggcgggaa ccgaggccct
      781 gcacggccgt tcgggcttcc tggtgccgcc ggtcgacggc cgcaccatca aggcgtcgac
      841 cttctccggc aacaagtggc ggtgggtcgc cgacgcggcc cccgacctct tcctgctgcg
      901 cacctccgtc ggccggtacg gcgaggagga ccacctgcac cgtgaggacg tcgagctggt
      961 cggcgcgtcc cggcgcgacc tcgcccaggc caccggactc accgcgcggc ccgtggacac
     1021 cgaggtcacc cgctggatcg gcggcctgcc ccagtacccg gtcggccacc tcgcccgggt
     1081 cgcccggatc cgcgaacacg tcggcgcgct gcgcgcgctg cgggtgtgcg gagcggccta
     1141 cgacggcgtc ggcatcccgg cctgcgtcgc cagtgcgcac cgggccgccg acgagatcgt
     1201 ccgcgacctg acggaggaga tcatcgccac gccgaccctg gttcggggca cggtgagcga
     1261 ggcgggacaa tagccgtatg agtgctcctg agactgtgac atccagcaag agtccgaacg
     1321 ccggaaagaa ggccaaggac ctcaacgagg tcatccgcta caccctctgg tcggtcttca
     1381 aactgcgcga cgtgctcccg ctggaccgcg ccgggtacgc cgaagaggtc caggagctgt
     1441 tcgaccggct cgcggcccag gacgtcaccg tccgcgggac ctacgacctg tccggactgc
     1501 gggccgacgc cgacctcatg atctggtggc acgccgagac cgcggacgag ctccaggagg
     1561 cgtacaacct cttccggcgc accaggctcg gccgcgcgct cgacccggtc tggtcgaaca
     1621 tggcgctgca ccggccggcc gagttcaaca agtcgcacat cccggcgttc ctcgccgacg
     1681 agacgccgcg cgactacatc agcgtctacc ccttcgtgcg ctcctacgac tggtacctgc
     1741 tgcccgacga ggaccgccgc cgcatgctcg ccgaccacgg caagatggcc cgcggctacc
     1801 ccgacgtccg cgccaacacg gtcgcctcgt tctcgctcgg cgactacgag tggatcctcg
     1861 ccttcgaggc ggacgagctg taccgcatcg tcgacctgat gcgccacctg cgcgcctccg
     1921 aggcacggct ccacgtgcgc gaggaggtcc cgttctacac gggccgcagg aagagcgtcg
     1981 cagacctggt ggccgggctc gcatagccag cgagacccgg accaccccaa cccggaaggt
     2041 cagaggacgg gcgcccggcg cgacgcgccg cccgtcgctc cagcgacacc gggttcggct
     2101 tcgggtcccg cgggagagtg cttcggcgct ctccggcggg acccgttcgg ttttcaggcc
     2161 ggggacaccg cgttccggtt cagtccaggg acaccgcgtt cggctccggc cgcgccgcgc
     2221 agtccgcgtc ccgctccggt gtccgcccgg tcagcaggta gcggcgcaga tgcccgtcga
     2281 cgcaggcgtt gcccgccccg ccgacgccgt gcgtaccggc gccccgctcg gtcacgagca
     2341 ccgcgcccgg cagccgccgc cgcagctcca gcgccccctc gtacggggtg accgcgtccc
     2401 gctccgccgc gaggatcagc accgggggca gctctccggg ccgcgtcccg acgtccagcg
     2461 ggcgctgccg gggcgtgggc cagaaggcgc agggcaggtt ggtgaacgcg ttgtgccagg
     2521 tcgctaacgg ggccgccgcc gcgagggccg tgtggtcgcg gtcccacacc gcgaagtcct
     2581 ccggccaggg ggcgtcgttg cactccaccg ccgtgtagac ggcccgctcg ttctcgtcgc
     2641 ccttcgccgt cacggcgtcc cgtgacgcct gggcgatcag cggctccgga ttgccccgcc
     2701 ggtactccga cagggccgtg gcccgcatcg cccagtagtc gtcgtagtac gcggcgtcca
     2761 ggaacgctgc ctggaactgc ccggtgccca ccgtcccgcc cgcgggccgg gcggcgatct
     2821 cggcgcggac ctcgtcgtaa ccgcgctgca cggcctcggg cgtggccccc agcccgtaca
     2881 cgtcgtggtg cgcggccacc cagcgccgga agtccgccca gcgggactcc agggcctgcg
     2941 actggccgag gttggagcgg taccagatcc gccgggggtc cggatcgacc gccgagtcga
     3001 agaccatgcg ccgtacgtgc gaggggaaca gggtggcgta cagcgcgccg aagtacgtgc
     3061 cgtacgaggc ccccatgaac gtcagttccg gctcgccgag cgccgcgcgc agcacgtcca
     3121 ggtcgcgggc gttgttgagc gaggtgtagt gccgcagggc gtctccggcg ttccgttcgc
     3181 agccgagcgc gtacgcccgc gcccgcgcga tccgcttctc cttgtcggcg gccgtggggt
     3241 ggacgggggc gtcggtgggc cgctccgcga acgcggccgg gtcctggcag gacagcgggg
     3301 ccgagtcccc cacgccgcgc ggggcgtagc ccaccaggtc gtacgcccgc gcgacctcac
     3361 gccactcgga catctcgccc agcagcggga aggtgatgct ggaggagccg ggaccgcccg
     3421 ggttgtagac cagggccccc tgccgtccgg ccgcgtcacc ggtggcggcg acgcggctga
     3481 cggtcagttc gatctgcggc ccgtcgggct ccgcgtagtc gagcgggacg gtgaccctgc
     3541 cgcacttcag cgtctccggc agcatctcct cctcggggca cgccccgaag tcgatcccgg
     3601 ccgccgccgc acgggccgcc gccaccgccg tcccccgggt ctcggcggcc ggcgggccgt
     3661 cccaggcgct cgccggtgtc acggtcaggg cggacaggac cagggagccg atcgttgcgt
     3721 acagcggtgc cgttctcacg cgcggggcct ctctgcggat gcggagaggg gatgctcggc
     3781 ggcggcacgg gcgaagtaaa gcaccgcgcc cgcatgtcac cgggaacgac tccgctgccc
     3841 ggcgcgccgg gccgacggcg cggcgcgtac gggtggaccg gcgcggcacg ggtcccggcc
     3901 cggccccggc gcggccccgg tccgggattc cggtgacccg ccgtcagccg gggccggtgc
     3961 tcacgaaccg ccgcctccgc agctggaccc gccgccgccc ccgcagctcg aaccgccgcc
     4021 gcccccgcaa ctggacccgc cgcctccgca tcccgacccc gatcccgcgc cgcagccgga
     4081 gccgccgccg catcccgacc ccgaaccgcc gccggtcgag cctccgcaac tcgtgtggcc
     4141 ggggctcgtg ctcgcgcacc acaccgtcgc cgccaccgcc gccgtgcccg ccgcggagct
     4201 cccggcggcg atcgggccgg accggtcgcc cggaccgcgc agccgggcag cggccaccag
     4261 gtggccctgg agcccgggat cgggcagcgc gcgcgggccc cggacggcga ccagacgcgc
     4321 gggcgactcc acggacgcgt gggcggcccg gaaaccctcc gcggcacggc ggcccgcccg
     4381 ggtgatccgc ttcgcggagg acacggccac gaccaggccg ctgatcgcgc cgagcaggat
     4441 cgcgggcacc accttcacga tgaacggcaa cggcatgtcg gacaggccgt ccaccatcgc
     4501 gtactggacg aaggtgagtc ccagggacac cgggaaggcg atcaggcagc acaccccctg
     4561 caccactccc caacggcgca gcggacgggt gtccgcgggc ggtacgagca gcccgcgcgc
     4621 cgccagcccg tcgccgatct cctgtaccgc cgggtgccgc atcaccgcgt cccgcaaggc
     4681 ggccagcgcg ccgctcggcg ctgcgcccag ctcctggagc acggcccgct ccaccgggtc
     4741 gtgcgcctgc ggacgctgta cggagacgat gccggggccg ccgatgagca gccgtccgtc
     4801 cgcgtgcagc gcggtcaggg cggtgtccac caccctgccc ggtccgccgc ccaggaacgc
     4861 cgcctcgtac aggtcgtgga cggcggggga cgggcccggc gcggggcggc ggcccttcgc
     4921 ggccagcagc cggacgatca ggagcagcga ggagacgacg accgcgaggg tcagcagagc
     4981 ggcgagtgcg ttcacggcgt tcacttcccc acgagagcgg cacgggccgc ccggaccagg
     5041 cgggcggcgc ggcgcggcgg gcggggcccg gaccggtcct gccaccagta cgtcagccgt
     5101 ctgcgcgcca ccggatcggc gggagcgccc gtgaccagca gccgctccgc gaaggcgagc
     5161 gcgtcgcgcc ggtagccgtc ggacatcgga cggcccgccg cgtaggcgag gaacgccgcg
     5221 cggtagccgt cgccgaggat ctccggcagc tcgggcgcca ccttcgccac gacgtcggcg
     5281 cgcttggccg ccagtgcccg gctctgcacg ccgagccgcc ggcggtcgaa gcccgcaggg
     5341 gccggggtcc cggcgaccag ggccgacagc agcgcggtct gggcgacggc ggtgcgctcc
     5401 cgcgcgcccg gcgcgacggg aacggcgggg acggcgacgg cggcgggacg ggtcgccgcc
     5461 gggcgcggcg cgggcgcggt ggacgcggtg cgcagcgtgg cgcggatcgt gtccagctcc
     5521 gccgccagtt ccggcgcggg cggaaaggcg tcgtcgcgct ccagcagcac gcccggcggg
     5581 tcgacccggg agcgcagctc gcgcagcacg tccaggaccg gggccgtcac cgggtgggcg
     5641 tgcgtgtcgt gccacacgcc gtccttctcg acgccgcccg ccacgtgcac gtacgcgatg
     5701 gcctcgaccg gcagttcgtc cagcgccttc gccgggtcct cgccgcggtt gacgcggttg
     5761 gtgtgcaggt tggccacgtc gatcagcagc cgcaccccgg tccgctcgac cagctccgcc
     5821 aggaaccggc cctcggtcag ctcctcgtcc ggccaggaga cgagcgccgc gatgttctcc
     5881 agggccagcg gtaccggcag cgcgtcctgc gcgatgcgca cgttctcgca cagcacctcc
     5941 agcgcgtccc aggtgcgcgg caccggcagc aggtgccccg cctccaggcg cggcgacgcg
     6001 gtgagcggcc cgccggcccg gacgaacgcg atgtgctccg tcaccagggg cgcgcccagc
     6061 agttcggccc gcgccgcgag acccgcgagc cgtccggcgt cggggcggtc cgcgccgccg
     6121 agaccgagcg agacgccgtg cggcacgacc gtcaccccgc gctcgcgcag ccgggccagg
     6181 gagttcggga ggtggtcgtc gcagatgttc tccgcgacgg cctcgaccca gtcgatcccg
     6241 ctcagcgcct ccacctcggc ggcgatctcc ggccgccagc cgattccgat gcccagctcc
     6301 atggtgtccc cctccacgct ctgttcaggt gtcatggccc cgctcgacgg cggcgaaccc
     6361 gaacacgggg acgttcagag caggatttga ggttccgcac cgcggcgtga aaagcgccgc
     6421 gcagtcccca ggcccgtccg ccggcccgtc ggccgacccg tcctccgcgc agtcagcggc
     6481 gcgacggccc cgggctcgtt cagcggcgca gcgccgggtg gtcggccacc accgtgcacg
     6541 accccggggc gatctcggtg aaaccggcgt cgcgcaccac cggcagcccg ctcgccgtca
     6601 gctcccgcca gcgtgcgggg cccggggtgg cgacggcgag cgggaacccg gcctcgcgcc
     6661 acgcggtgcg ctccgcgtcc gtcagctccc accaggcgag ctgggcggcg tgcccggcct
     6721 gggccatcgt cttgcccgcc gacatgtcca gctccgggtt gagccacagc accgggcccg
     6781 ccgggtcggg cgcggcgggc ggctccggat cgtccagatc ggtgccggag acctggagct
     6841 tggccagctc cttgggccag ccgtccagcg ggaccggcgg gaacacccgt acctccgcgc
     6901 tctcgccggt gaccgtgatg ccgggcaggg ccgccgcctt gcgccactcc gcgccgcgtg
     6961 cccggcgcac caccttgcgg atgcgcgcgt cctgccagtc ccgcatcgcc cgcgcccact
     7021 cgccctcgcc cagcgaccgc tcgtcgcaga ggatcgtcag gaccgcgcgg gccgccgtgc
     7081 gcagcgcgtc cgtccgggcc ggcgggtccg tcttctccag gtgcaccacg agcggcagca
     7141 cgtactgcgg ggcctcgtca cgcggggtgg tcccgtcgcg gaacgggctc tcggcgggcg
     7201 cgccgacgcc gacgccggtg gcgggtgcgg tcgtgggggc gtcggtcgcg gggatgtcgt
     7261 tgctgctcac cgtcccagtc tgccaggcgg cgaacgtcac cttcttggcg gaacgagacg
     7321 gtccgggtga ggatgctccg tatgaagagc gatctctttt ccagcgagca catggcgcag
     7381 ccggcgaccg cccccggtat gaccctccag aacgccaaat ccatcaagta cgccgtcgac
     7441 ggcgagatgc acgcccggca gggatcgatg atcgccttcc gcggcgacct ccagttcgag
     7501 cgcaagggcc agggcctcgg cggcatgctg aagcgcgcgg tcaccggcga ggggctggcg
     7561 ctcatggcgg tccggggtcg gggcgaggcg tggttcgcgc acgaggccgc caactgcttc
     7621 gtcgtcgaga tggagcaggg cgacgtgctc acggtcaacg gccgcaacgt cctgtgcttc
     7681 gacgccaccc tctcctacga gatcaagacg gtgaagggcg ccgggatgac cggcggcggg
     7741 ctcttcaaca gcgtcttcag cggtcacggc aagctcggcc tgatgtgcga cgggcatccc
     7801 atcgtcatcc ccgtcacggc ggatcagccg gtgtacgtgg acaccgacgc cgtcgtcggc
     7861 tggagccggc agctctccac caccctgcac cgctcgcaga gcgtcggctc gatggtccgc
     7921 ggcggctccg gcgaggccgt gcagctgatg ctccagggcg aggggttcgt gatcgtccgg
     7981 cccagcgagc tgaagccgga gaagaccgcg aactgaccgg gcggccggac ggcgggcggc
     8041 cccgcccgtt cggcgttccg tccgccgtcg tctcagccct ccatcagctc cgagaccttc
     8101 acgaagcggt agccgcgctt ccgcagctcg gggacgatgg tgcgcaccgc ctccccggtc
     8161 gtcggggccg cgctgcgcgt gcagtgcatc acgacgagcg aaccgggctt caccccgtcc
     8221 agcacctgcc gcgccaccgc gtccgcgtcc gtggcgaacg cgtcgccgct caccacgtcc
     8281 cactgcaccg ccgtcacctt cgccggggcc agcgcgcgca gggcggcgtc gtcgtagcag
     8341 ccgcccggga accggaagta cggcaccacg ttgcgcgcgc ccgccgtccg gaacgcggtg
     8401 aacgcccgcg ccagctcgcc cgccatgtcg gccccggcga cctccggcag cccgtagcag
     8461 ggggagctga aggcgtagtg gctgtaggag tggttggcga tctcgaacag cgggtccgtg
     8521 ccgatgcgct tcgcctgggc cgggtactcc tcggcccagc gtcccgtcat gaagacggtc
     8581 gaggggacct tcagccgccg cagcagcgcg atcagctccg ggttgtcgaa gcgctcgccc
     8641 gaggccgccc tcgggccctg gtcggcggtc atgtcggcgt cgaaggtgag cgccacgacc
     8701 ttgtccgccg ccttcccggc cgggccgccg gccgccgccc gccgttcgaa gaccgggacg
     8761 cggcccgccg ggccgggcgc catggtgggg ggcttgcgga cggcggggga cgccggggag
     8821 gcgggggagg gcgaggccgg ggccgtctcg gcgcggggac cggccgggct tccgcacccg
     8881 accagcacac cgctcagcag tgctcccgcc accgcccctg tcagcgctga gatagtcatt
     8941 ttccgtgctg atctgatcat tcacggaaaa tatcggacat tacgtcaggg ggcgaccggc
     9001 aaatggatca attggggacg acctgtgggc ggggcctgat ggtgtgaagc cgacacgccg
     9061 tcaggagcgg cgctgctctt cggccacgag cgcgagcacg cgctcgcgct ccgcgcggcc
     9121 ccaggtctcc ttggtggcga ggatgccgac gaggccgacg atcgcggccc cgaggtagag
     9181 cagtgcggcg ccgggccagc cgaaggctcc gacgagggcg gcggcgagca ggggtgcgaa
     9241 gccgccgacg gctgccgcga cctggtagcc gagcgaggct ccggaggtgc gcgtggcggt
     9301 gcggaactgc tcggtgagca gggagccctg cactccggtg aaggaggcgt ggacgactcc
     9361 gatcccgatg acgaacacgg cgatgaccag ggccggttga gcggtgttgg tgagcaggta
     9421 catcgggaac gcgaacagga tggctgccgt gcaggccgcg atgtagaggg ggcggcggcc
     9481 gatgcggtcg gtgagcgaac cggccaggaa ggtgaccgcg atggccagga tgctggcggc
     9541 cgtgatcgcg ccgagggcca cgggccgcag gtccgggtgg cgttcggtga tgtagctgag
     9601 caggtaggtc gcggtggagt agtaggcgaa cgattcgacg acgcgcaggg cgatgacccg
     9661 cagaatgctc tgccagtcgt cccgaagggt tgcgaggaaa ggattcttct cggtcgtgcc
     9721 gctggcctgg gcctcctgga actcagggga ttcggagagc cgggagcgca cgatcagcgc
     9781 gacgatgacc agaacggcgc tgagcaggaa cggagtccgc cactgccagt cgccgctgag
     9841 cgtggcgctc caggcgaaca caccgctggc gagggcgatt cccagagggt tgccggactg
     9901 cgggatcgcc gcgaacatgc cgcggcggtg ccagggcgcg tgttcgtagg ccatggtgat
     9961 ggccccgccc cattccgcgc cgaaggccag gccctggacc atgcggatca gcacgagcag
    10021 gaccggcgcg agcacaccga cctgctcgta cgtcgggagc acgccgatga ggaacgtggc
    10081 cgcgcccatc acgatcatgg cggcgaccag gacgggcttg cggccgagct tgtcggcgaa
    10141 gtaaccgccg atcgcgccgc cgagcggacg catcacgaag ccgaccgcga gggtggcgaa
    10201 cgacaggagc gtgccgacga aacggtcgct ctcggggaag aagacggccc cgaagtagag
    10261 cgcggaagcg gttccgtagg ctatgaagtc gtagttctcg acgctggtgc cgacggctgc
    10321 ggcgatcgcg gtcttcacgc ggtccttggt gccgagcacg cttggctgtc cggcgatggt
    10381 gctggtcgac atgggttgct ccttgcgggg gtgcgccgga tcggcgtgcg cggggtgcgg
    10441 agtggccggg cctgagcggg tcccttggcg atgaggtgtt tccgggtgct tccggatctg
    10501 cttccgggtc tgtttccggg tctgacgagg tggcgcgggg gaggcgttcg ggcgggtcgc
    10561 ccgcagccgc gcggcgcggc tgcgggcgag ttccggtcct gctccccggt gtgtcaggtc
    10621 ttcggggtca ggctgtcgag cgcgttgagc ttggcgaccc tgcggcgggt gtcctcgtcg
    10681 tcgtcgaagg tggcggccag gaactcgcgg acgatgtcgg gcaccagggc gtgcgggacg
    10741 agccaggcgc ccatggcgat ggcgttggcg tcgtcgtgtt cgacggcctg gtgggcggag
    10801 tacggctcgt gggccaggcc gcagcggatg cctggcagct tgttggcggc catgacggcg
    10861 ccctgaccgg taccgcagac gagcacggcc cgatccgccg tgccggagag caccgggtcg
    10921 caggtggccc gggtgatgtc ggggaagtcg acgggatcgg agccgtgagt gccgcagtcg
    10981 acgacctcgt gtccgaggtc ctctatggtc cggcgtacgg cctccttgag cggatacccg
    11041 gcgtggtctg ctccgagggc gatcttcatc tgctggtcct tacgtggcga gcggggccgg
    11101 gatcggcccg gtgggatggg ggagggaggc cgcgccgctc ggcggcgagt gtgctcatcc
    11161 ggtttcgcgt ccggtttctc gtccggcttc gcgtccggtg tcccggccgg agcggtcgac
    11221 gaaggaagtg gcgacgcgct gcccggtcgc gggacgcccg gcgaggaggt cgacgatccc
    11281 gcgcacaccg aggcggccga tc
//
