LOCUS       NZ_ML123034            10963 bp    DNA     linear   CON 23-MAY-2024
DEFINITION  Streptomyces sp. ADI96-02 scaffold001, whole genome shotgun
            sequence.
ACCESSION   NZ_ML123034
VERSION     NZ_ML123034.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. ADI96-02
  ORGANISM  Streptomyces sp. ADI96-02
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 6907715)
  AUTHORS   Guerrero-Garzon,J.F., Zehl,M., Urban,E., Schneider,O., Ruckert,C.,
            Busche,T., Kalinowski,J. and Zotchev,S.B.
  TITLE     Streptomyces spp. from the marine sponge Antho dichothoma: analyses
            of secondary metabolite biosynthesis gene clusters and some of their
            products
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6907715)
  AUTHORS   Guerrero-Garzon,J.F., Zehl,M., Urban,E., Schneider,O., Ruckert,C.,
            Busche,T., Kalinowski,J. and Zotchev,S.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2018) CeBiTec, Bielefeld University, Sequenz 1,
            Bielefeld 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64.8x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003846185.1-RS_2024_05_23
            Annotation Date                   :: 05/23/2024 03:48:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,089
            CDSs (total)                      :: 6,032
            Genes (coding)                    :: 5,843
            CDSs (with protein)               :: 5,843
            Genes (RNA)                       :: 57
            tRNAs                             :: 54
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 189
            CDSs (without protein)            :: 189
            Pseudo Genes (ambiguous residues) :: 0 of 189
            Pseudo Genes (frameshifted)       :: 68 of 189
            Pseudo Genes (incomplete)         :: 155 of 189
            Pseudo Genes (internal stop)      :: 10 of 189
            Pseudo Genes (multiple problems)  :: 42 of 189
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:00:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 175916
            Orig. end                         :: 186879
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            ML123034.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Zotchev Lab, Department of
            Pharmacognosy, University of Vienna.
            Annotated using prokka v1.11 from http://www.vicbioinformatics.com.
FEATURES             Location/Qualifiers
     region          1..10963
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="2"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10963
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10963
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5963](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5963)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(98..1138)
                     /locus_tag="EES43_RS00740"
                     /old_locus_tag="EES43_00755"
     CDS             complement(98..1138)
                     /EC_number="1.1.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (19-268). E-value: 1.3e-11.
                     Score: 36.1. Matches aSDomain:
                     nrpspksdomains_EES43_RS00740_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019761872.1"
                     /locus_tag="EES43_RS00740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_00755"
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="WP_124266824.1"
                     /sec_met_domain="ADH_N (E-value: 2e-28, bitscore: 96.9,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 1.8e-20, bitscore:
                     71.8, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVAAYAAPSAKAPLERTTIERRPVGEFDVLIDIKYSGICHSDI
                     HQARDGWGEGIFPMVPGHEIAGIVAETGSGVTRFKVGDRVGVGCMVDSCGRCDACASGR
                     EQHCAEGNTPTYNALDRNGEPTYGGYSTHIVVREGFTVGIPNGIALDEAAPLLCAGITT
                     YSPLKRWGAGPGKKVAVLGLGGLGHMAVKIAHALGAEVTVLSQSLRKKEDGLKLGADHY
                     YATSDPRTFEELAGRFDIILSTVSAPLDFGAYLGLLRTDGILVNVGAPEEPISLNLFSL
                     IGGNRSLAGSLIGGIAETQEMLDFCAAHGLGAEIEVIEAARVNEAYERVLASDVRYRFV
                     IDAATI"
     aSDomain        complement(335..1081)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EES43_RS00740_PKS_ER.1"
                     /evalue="1.30E-11"
                     /label="EES43_RS00740_PKS_ER.1"
                     /locus_tag="EES43_RS00740"
                     /protein_end="268"
                     /protein_start="19"
                     /score="36.1"
                     /tool="antismash"
                     /translation="TIERRPVGEFDVLIDIKYSGICHSDIHQARDGWGEGIFPMVPGHE
                     IAGIVAETGSGVTRFKVGDRVGVGCMVDSCGRCDACASGREQHCAEGNTPTYNALDRNG
                     EPTYGGYSTHIVVREGFTVGIPNGIALDEAAPLLCAGITTYSPLKRWGAGPGKKVAVLG
                     LGGLGHMAVKIAHALGAEVTVLSQSLRKKEDGLKLGADHYYATSDPRTFEELAGRFDII
                     LSTVSAPLDFGAYLGLLRTDGILVNVG"
     gene            1311..2225
                     /locus_tag="EES43_RS00745"
                     /old_locus_tag="EES43_00760"
     CDS             1311..2225
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459219.1"
                     /locus_tag="EES43_RS00745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_00760"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_124271727.1"
                     /transl_table=11
                     /translation="MDDQPVPSGPETFDRRAELSEFLRTRRARLKPQDVGLPEFGRHRR
                     VPGLRREELAQLAGVSVAYYTRLEQGNGRNVSAEVLDAIAHALRLTDAEHAHLTHMTKP
                     ARHKKKRRPARVQQVRLGLRQLLDTMDGVPAYVSGARTDILAWNPLAAALFGDWGALPP
                     KERNWARLVFLSPAYQDLFVDWDAKASDTVSYLRLYAGCHPDDPELSALVGELSVKSEE
                     FRRLWATHNVKEKGHGVKLIRHPLAGELSLSYETLHLPDDEEQFLVTFHGEPGSASAEA
                     LRMLASWGSDAIRADLTRIPPGS"
     gene            complement(2258..>2995)
                     /locus_tag="EES43_RS00750"
                     /old_locus_tag="EES43_00765"
                     /pseudo=""
     CDS             complement(2258..>2995)
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003949011.1"
                     /locus_tag="EES43_RS00750"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_00765"
                     /product="class I SAM-dependent methyltransferase"
                     /pseudo=""
                     /transl_table=11
                     /translation="RLAALYDRLDPDRGDLDAYLRTARDLGARRVLDIGCGTGVFALRL
                     ADRAVEVVGLDPDRASIDAARAKPGSERVRWIRGDATSLPPLRVDLVTMTANAAQEIIG
                     RSLWRRTLRGAREALRPGGRLLFESRDPAMCAWQEWNRETSRRVTAVPGVGEVESWVEL
                     IEADLPLVSFRWTYVFAADGAVLTTRSTLRFRERREIEKDLAEAGFLLEDVRDAPDRPG
                     REFVFLARRPERTHARAAATEHG"
     gene            complement(3160..3807)
                     /locus_tag="EES43_RS00755"
                     /old_locus_tag="EES43_00770"
     CDS             complement(3160..3807)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606639.1"
                     /locus_tag="EES43_RS00755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_00770"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_124266826.1"
                     /transl_table=11
                     /translation="MTKQERATRTRQALIRSAAEAFEQHGYAQAGLTLISSGAGVSTGA
                     LHFHFENKAAVAAAVVAEASRGLSDAHDAVRRGTDTALQALVDISHALSALLRRDTVVR
                     AGFRLSCDEECAEPALRAQWRCCVRDLLDEAAVAGALADGVSRADVTAATVAVTTGFEV
                     LGRHDPQWLAAERLTGYWRLLLPGLAVAEVRARLNPAGTAPSYAPEAVGGGS"
     gene            complement(4006..4869)
                     /locus_tag="EES43_RS00760"
                     /old_locus_tag="EES43_00775"
     CDS             complement(4006..4869)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018515158.1"
                     /locus_tag="EES43_RS00760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_00775"
                     /product="NAD(P)H-binding protein"
                     /protein_id="WP_124271728.1"
                     /transl_table=11
                     /translation="MLLVTGATGPVGREVAALLAAAGPVRILARNPGRVTVRGPGVEVV
                     AGEYADRGSLDRALKGVSGVFLVTNSPAEPDDERVAGAARAAGVGRLVKLSMMAVEEPG
                     ADDFITRRQRGNEQAIRESGVPWTFVRARTFMSNTLSWAPGIRSAGVVRALYGDSPVAC
                     VDPRDVAAVAVAVLTGTGHEGRAYPVSGPEAITARDRTARLSEVLGRPLRYEELPLEQA
                     REALFAKYPAPVAQAFVESAERQRAGAKAAVVPTVRELTGRPARTFRTWAADHADAFAA
                     DPPASG"
     gene            complement(5001..5963)
                     /locus_tag="EES43_RS00765"
                     /old_locus_tag="EES43_00780"
     CDS             complement(5001..5963)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971211.1"
                     /locus_tag="EES43_RS00765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_00780"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_124266827.1"
                     /sec_met_domain="AfsA (E-value: 1.4e-26, bitscore: 90.8,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPEAAVLIDPGTVEVPFVEHVPDGGPVHPVRMELVHRSRAEDAFA
                     RSWVRRGPDRFSVAAVLPHDHPFFAPVPGDRHDPMLLTEAMRQAAMLAFHAGYGVPLGH
                     HFLMADLDFACRLSRLRVAGGPAEVDIEVTCSELKWRGGTPVQGRVDWVVLRGGLVAAT
                     GMGATRFTSPQVYRRMRGGSATPGTCIPDAPSVSPVPAGRGRVEDVVLTEGPLEGVWEL
                     RVDTRHPTLFQRPNDHVPGMLLLEAARQAACLVAGPGGFVPAEGSTRFHRYAEFTSPCW
                     IRAVVLPGAAPDTVTVQVTGHQDRSPVFSTVLSGPRLTG"
     misc_feature    complement(6021..6041)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.91"
                     /tool="antismash"
     gene            6126..6767
                     /locus_tag="EES43_RS00770"
                     /old_locus_tag="EES43_00785"
     CDS             6126..6767
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606642.1"
                     /locus_tag="EES43_RS00770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_00785"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_124266828.1"
                     /transl_table=11
                     /translation="MAKQDRAIRTRRAILMAAAETFEKHGYQAATITQILKVAGVTKGA
                     LYFHFTSKEDLALGVLDAQEPPESVPEQLLKLQELIDLGLLFSHRLRTSLLARAGVRLS
                     MDQQAQGLDRSGPFLRWQEATHRLLAQAKHNGELLTHVNPTETADLYVAAFAGIQAVSQ
                     TLTNYEDLQKRYLTLQHHVLPSITTPSVLAALDLTPQRTDELVRLAPYPL"
     gene            complement(6801..7412)
                     /locus_tag="EES43_RS00775"
                     /old_locus_tag="EES43_00790"
     CDS             complement(6801..7412)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459226.1"
                     /locus_tag="EES43_RS00775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_00790"
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="WP_124266829.1"
                     /transl_table=11
                     /translation="MTSTLSTAAAGFATGLSLIVAIGAQNALVLRQGVRRDAVLPVVAI
                     CALSDAVLIALGVAGVGAVVVAWPSALTVVALLGGVFLICYGVLAARRALRPSALLAGD
                     APNGTRRGPVLACLAMTWLNPHVYLDTVFLLGSVAADRGSGRWVFGLGAVLASLCWFAA
                     LGFGARLLSGVLARPSSWRVLDGLVAAMMIGLGAKLMLGA"
     gene            7480..8436
                     /locus_tag="EES43_RS00780"
                     /old_locus_tag="EES43_00795"
     CDS             7480..8436
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1031: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018515154.1"
                     /locus_tag="EES43_RS00780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_00795"
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="WP_124266830.1"
                     /transl_table=11
                     /translation="MMPELPLDQVRTLLAVVDEGTFDAAASVLHVTPSAISQRVKALEQ
                     RTGRVLLMRTKPVRPTESGEVVVRFARQLARLERDAMASLGMSGPGEPARLSIAVNADS
                     LATWFLPALRRVPETAEVCYELRREDQDHTSALLREGRVMAAVTSSPEAVTGCSVRGLG
                     VMRYLPVAGSGFADRWFGPRAGTTPRDLLAAAPVVGFDRRDDLQDTFVRGLGGDRASGR
                     RHYVPTSEGFVAAVAAGMGWGMVPDVQAEALLAAGRLVDLAPGRTVDVPLYWQQWKLDS
                     PSLAAVAGAVTAEAAVSLRPGGGPGAGPAGHGRSGAS"
     gene            8540..9214
                     /locus_tag="EES43_RS00785"
                     /old_locus_tag="EES43_00800"
     CDS             8540..9214
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971207.1"
                     /locus_tag="EES43_RS00785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_00800"
                     /product="response regulator transcription factor"
                     /protein_id="WP_124266831.1"
                     /transl_table=11
                     /translation="MRVLLVEDEDDLRAVIAAGLREAGFAVDCAPDWPDADVMLHVTAY
                     DCVVLDRMVPFGDTLVPLERRRRTGWSVPVLCLTALDTLDDRLFGLESGADDYLAKPFA
                     MRELVLRVRSLTRRATERLPAFLGCADVVMDLARREVRRGGVLLSLSPKEYAVLQQLLV
                     NAESVVTRTGLLEHCWDEMADPVSNVVDAVIAGVRRKLGSPGLVHTVRGQGFLMSAGRG
                     PS"
     gene            9214..10551
                     /locus_tag="EES43_RS00790"
                     /old_locus_tag="EES43_00805"
     CDS             9214..10551
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455769.1"
                     /locus_tag="EES43_RS00790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_00805"
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="WP_124266832.1"
                     /transl_table=11
                     /translation="MAGRPERRRRSPARRRLRRLQLRLTAAYTLITALGLSCLCWLVIR
                     TDDRSRQASEYDEMRRRASVAASLVYYEEDRLRLDGLQDDEATTGTPQILVLEAPPGGP
                     HAFVFRGRVSRFDVADATAERVARSAMRADDVVRSTGHDRSGDPVYLLAVPFHHDATDA
                     MAGAAVAVGDPERGSAEHRSLILSLAAGGGALTALAGLTGHVLSGRSMRPAWQALEQQE
                     RLLADAAHELRTPVAVMRGSVEVAEGGAGGLGVHLPRIRRAADRMADVVENLLTRGQLD
                     AAVDSVRPTPLRLDQLVEEVCADLPDGGHRLELCLEESVVEADAGLVRVAVRNLLDNAA
                     RHGRTPGAPGGADLLVSVLGPSVSVADRGPGVEPGRLPELMERFRSPGGGTGIGLSLVH
                     RVATVHRGSLTVRARPGGGAVFVLRLAPGRSRRRRAGGRGGVRARR"
     misc_feature    10621..10634
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.67"
                     /tool="antismash"
ORIGIN
        1 gatccgcgcc cggggctttc gacctgagcg cggatcgcgg tgttcagact ccgcccgcgc
       61 cgccgtaccg gcccggccgg tacggcggcg agacgcgtca gatcgtcgcg gcgtcgatca
      121 cgaagcggta gcgcacgtcg ctcgcgagca cccgctcgta cgcctcgttg acacgggccg
      181 cctcgatcac ttcgatctcg gctccgagcc cgtgggccgc gcagaagtcc agcatctcct
      241 gcgtctccgc gatgcccccg atcagcgatc cggccagcga gcggttgccg ccgatcagcg
      301 agaagaggtt cagcgagatc ggctcctcgg gggcgccgac gttgaccagg attccgtcgg
      361 tccgcagcag gccgaggtag gcgccgaagt ccagtggcgc ggatacggtg gagaggatga
      421 tgtcgaagcg gccggccagt tcctcgaagg tcctggggtc gctggtcgcg tagtagtggt
      481 cggctcccag cttcagaccg tcctccttct tgcgcagcga ctgggagagc acggtgacct
      541 ccgcgccgag cgcgtgtgcg atcttgaccg ccatgtggcc gaggccgccc agcccgagga
      601 ccgcgacctt cttgccgggg ccggctcccc agcgcttgag cggcgagtac gtggtgatgc
      661 ccgcgcacag cagcggcgcg gcctcgtcga gtgcgatccc gttcggaatg ccgacggtga
      721 agccctcgcg gacgacgatg tgcgtcgagt agccgccgta cgtgggctcg ccgttccggt
      781 cgagcgcgtt gtaggtcggc gtgttcccct cggcgcagtg ctgctcgcgg ccggaggcgc
      841 aggcgtcgca gcgaccgcac gagtcgacca tgcacccgac gccgacgcgg tcgccgacct
      901 tgaacctcgt cacaccggag cccgtctcgg cgacgatgcc cgcgatctcg tggccgggca
      961 ccatggggaa gatcccctcg ccccagccgt cgcgggcctg gtggatgtcg gagtggcaga
     1021 ttcccgagta cttgatgtcg atcaggacgt cgaactcacc caccgggcgg cgctcgatgg
     1081 tcgtacgctc cagcggggcc ttggcgctgg gggcggcgta agcggcaacg gtggtcatgc
     1141 cggtggttct cctctggtga tgcgtgcgga actctggtgg tgcgtctgga acgccttcca
     1201 gcatgccgat caaccgcgcg ttcacccagg tcaccgcttt gcctaggacc agcggtccta
     1261 ctactggcgg ggacagcctc ccggtcgtgg gaccaggcat catggaggcc atggacgacc
     1321 agcccgtacc ctccggcccc gagacgttcg accggcgcgc ggagctcagc gagttcctgc
     1381 gcacccgtcg ggcccggctc aagccccagg acgtcgggct gcccgagttc ggccggcacc
     1441 gcagggtgcc gggactgcgg cgggaggagc tggcgcagct ggccggggtg tcggtggcgt
     1501 actacacccg cctggaacag ggcaacgggc gcaatgtgtc ggcggaggtg ctggacgcca
     1561 tcgcgcacgc cctgcggctg accgacgccg agcacgcgca tctgacccat atgaccaagc
     1621 cggcgcggca caagaagaag cggcggccgg ccagggtgca gcaggtgcgg ctcggcctgc
     1681 ggcagctcct ggacacgatg gacggcgttc cggcgtacgt atccggcgcg cgcaccgaca
     1741 tcctcgcctg gaacccgctg gcggcggcgc tgttcggcga ctggggcgcg ctgccgccca
     1801 aggagcgcaa ctgggcccgg ttggtgttcc tctcccccgc ctaccaggac ctgttcgtgg
     1861 actgggatgc caaggcctcg gacacggtca gctatctgcg cctgtacgcg ggctgccatc
     1921 ccgacgatcc cgagctgtcg gcgctcgtcg gtgaactcag cgtcaagagc gaggagttca
     1981 ggcggctgtg ggccacccac aacgtcaagg agaagggcca cggcgtcaag ctgatacggc
     2041 atccgctcgc cggcgagctg tccctgtcgt acgagacgct gcatctgccg gacgacgagg
     2101 agcagttcct ggtgacgttc cacggcgaac cgggatcggc gtcggccgag gccctgcgga
     2161 tgctggcgag ttggggatcg gacgcgatcc gcgccgacct gacccgcata ccgccggggt
     2221 cgtaggcgac gggccccggg cgggcgtcag aggtgtgtca cccgtgttcc gtcgcggctg
     2281 ctctcgcgtg cgtgcgctcc gggcgtcggg cgaggaagac gaactccctg cccgggcggt
     2341 ccggtgcgtc gcggacgtcc tccaggagga accctgcctc ggccaggtcc ttctcgatct
     2401 cgcggcgttc acggaagcgg agcgtcgacc gcgtggtgag gaccgcgccg tcggcggcga
     2461 agacgtaggt ccagcggaac gagacgagcg gcaggtcggc ctcgatcagc tcgacccagc
     2521 tctcgacctc gccgaccccg ggtaccgccg tgacccggcg ggacgtctcg cggttccact
     2581 cctgccaggc gcacatggcc gggtcccggc tctcgaagag cagacggccg ccggggcgca
     2641 gtgcttcgcg ggccccgcgc agcgtccgcc gccagaggga ccgtccgatg atctcctggg
     2701 cggcgttcgc cgtcatcgtc accaggtcga cgcgcagcgg cggcagcgag gtcgcgtcgc
     2761 cgcggatcca gcggacgcgc tcgctgcccg gtttggcgcg ggccgcgtcg atggaggccc
     2821 ggtcgggatc gaggccgacg acctcgacgg cgcgatcggc cagtctcagg gcgaagacgc
     2881 cggttccgca gccgatgtcc aggacgcgac gcgcgccgag gtcgcgcgcg gtccgcaggt
     2941 aggcgtcgag atcgccgcgg tccgggtcga gccggtcgta gagcgcggcg aggcgcgcga
     3001 gtgtgagcgt ggggcgcggg tgtcagccat gcggccggaa actaccggga gcggggggcc
     3061 gggcccagcc gtttcccacg cggcggggac agggcccggt ccgcccctac gcggccgtga
     3121 gcgcggggcg gtcgccgcac gggcccggcc gtgctgctct cacgacccgc cgccgacggc
     3181 ctcgggcgcg tacgacgggg cggtccccgc ggggttcagc cgggcacgga cctcggcgac
     3241 ggccaggccg ggcagcagca gacgccagta gccggtgagc cgttccgcgg cgagccactg
     3301 cgggtcgtgc cgccccagca cctcgaaccc ggtcgtgacg gccaccgtcg ccgcggtcac
     3361 gtccgcacgg gagacgccgt cggcgagcgc gccggcgacg gcggcctcgt ccagcaggtc
     3421 acggacacaa caccgccact gggcgcgcag agcgggttcg gcgcactcct cgtcgcaact
     3481 gagccggaat ccggcccgga ccacggtgtc ccggcgcagc agggcgctga gggcgtgcga
     3541 gatgtccacg agcgcctgca gcgcggtgtc ggttccgcgc cggacggcgt catgggcgtc
     3601 gctcagaccg cgcgacgcct ccgccacgac cgcggcggcg acggcggcct tgttctcgaa
     3661 gtggaagtgg agcgccccgg tgctcacgcc cgcgccggag ctgatcagcg tcagccccgc
     3721 ctgggcgtat ccgtgctgtt cgaacgcctc cgcggcggac cggatcagcg cctggcgcgt
     3781 acgggtggca cgttcctgct tggtcaccgg agcctcccag ggaagtcccg aacggccgga
     3841 gcgccgcccg ggcggagagc cgggcggacc ggggattgcg ggccgagcgg gtgagacggc
     3901 atcatgggag ttatcaaacc aactcagcgg tttcatttcc agcgaagagc cggaccgccg
     3961 cgcggccgtc cgcaaccggg ccaactcccg tacgggcgac cggcttcagc ccgatgccgg
     4021 cgggtcggcg gcgaaggcgt ccgcgtggtc ggcggcccag gtgcggaacg tcctggcagg
     4081 acggcccgtc agttcccgca cggtggggac gacggccgcc ttggctcccg cgcgctgacg
     4141 ctccgcgctc tcgacgaacg cctgggccac cggggccgga tatttcgcga agagcgcctc
     4201 gcgcgcctgt tccaggggga gttcctcata gcgcaggggc cttcccagca cctcggagag
     4261 ccgcgccgtc cggtcccgcg cggtgatcgc ctcgggaccg gagacggggt acgcccggcc
     4321 ttcgtggccg gttccggtga gcacggcgac cgcgacggcg gcgacgtccc gcggatcgac
     4381 gcacgcgacc ggcgagtccc cgtacagggc tcgcaccaca ccggcggacc ggatacccgg
     4441 agcccaggag agggtgttgg acatgaacgt cctggcacgg acgaacgtcc agggcacgcc
     4501 ggactccctg atggcctgct cgttgccgcg ctggcgccgg gtgatgaagt cgtccgcgcc
     4561 gggctcctcc accgccatca tggacagctt caccagacgg ccgacgcctg ccgcgcgtgc
     4621 cgctccggcg acccgctcgt cgtccggttc cgccgggctg ttcgtcacca ggaagactcc
     4681 gctgacgcct ttcagcgccc ggtcgaggga gccgcggtcc gcgtactccc ccgcgacgac
     4741 ctcgacaccg ggcccccgta ccgtcacccg tcccggattc cgcgccagga tccgcaccgg
     4801 tcccgcagcc gccagcagtg cggcgacctc gcggcccacc ggtccggtcg cccccgtcac
     4861 cagaagcacc atccacctct tcctctcgtc ccgacaccat cacgcggggc gcgtcgtcgc
     4921 gccgccgcgg caccgccttc gccgggcggc cggctcaggg cctgtcggcg cggccccgtc
     4981 gcggcccgga ccgggctcgg tcagccggtg aggcgcggcc ccgagagcac ggtggagaac
     5041 acggggctgc ggtcctgatg gcccgtcacc tgcacggtca ccgtatcggg agccgcgccg
     5101 ggcaggacca ccgcccggat ccagcagggg ctggtgaact ccgcgtagcg gtggaaccgc
     5161 gtgctgccct ccgccggcac gaagccgccc ggccccgcga ccaggcacgc ggcctgccgg
     5221 gccgcttcga gcagcagcat gcccggcaca tggtcgttgg gccgctggaa cagggtggga
     5281 tggcgggtgt ccacgcgcag ttcccagacg ccctccagcg ggccttccgt caggacgacg
     5341 tcctcgacgc ggccccggcc cgccggcacc ggcgagaccg agggcgcatc gggtatgcag
     5401 gtgcccggtg tcgcggagcc gccgcgcatc cgccggtaca cctgcgggct ggtgaacctc
     5461 gtcgcgccca ttccggtcgc cgccaccagt ccgccgcgga gcaccaccca gtccacgcgc
     5521 ccctgcaccg gcgttccgcc gcgccacttc agctcggagc acgtcacctc gatgtcgacc
     5581 tcggcgggcc cgccggcgac gcgcaggcgg gagaggcggc aggcgaagtc gaggtccgcc
     5641 atcaggaagt gatggcccag cgggacgccg tagccggcgt ggaaggcgag catcgccgcc
     5701 tgccgcatcg cctcggtcag gagcatcggg tcgtggcggt ccccgggaac gggcgcgaag
     5761 aacggatggt cgtgcggcag gacggccgcg accgagaacc ggtccgggcc ccggcgtacc
     5821 cagctccgcg cgaaggcgtc ctccgcccgg ctccgatgca ccagttccat cctcacgggg
     5881 tgcaccggcc ccccgtccgg gacgtgctcg acgaacggga cctctaccgt cccgggatcg
     5941 atcaggactg ctgcttcggg cataactccc cccaggggtc atgtgttggc gagtgggttg
     6001 ttccagcggt gcgctgataa catacagact aagcggtttt tttctacccc ggcggggagg
     6061 tccccggccc ccatgcccga ctcccggtcg caccaccacc ggcccagcgc aacgaaggag
     6121 gcaggatggc caagcaggac cgggcgatcc gtacccgccg agcgatcctg atggcagcag
     6181 cggagacctt cgagaaacac gggtaccaag ccgccacgat cacccagata ctcaaggtcg
     6241 ccggcgtcac caagggcgcg ctctacttcc acttcacatc caaggaagac ctcgcactcg
     6301 gcgtcctcga cgcccaggag ccgccggaat ccgtcccgga gcaactgctc aagctccagg
     6361 aactcatcga tctggggctg ctcttcagtc atcgcctgcg caccagcctc ctcgcccgcg
     6421 ccggcgttcg cctgtccatg gaccagcagg ctcagggcct cgaccgcagc ggccccttcc
     6481 tccgctggca ggaggccacc caccgcctcc tcgcgcaagc caaacacaac ggtgaactcc
     6541 tcacccacgt caaccccaca gagacggcgg atctctatgt ggccgcgttc gccggcatcc
     6601 aggcggtctc ccagaccctg accaactacg aggatctcca gaagcgctac ctcacgctcc
     6661 agcaccacgt gctgcccagc atcaccacgc cctccgtgct cgcggcgctc gatctgaccc
     6721 cccagcgcac cgacgagctg gtccgcctcg cgccgtaccc cctctgacgc cgcgccgcgc
     6781 cgcggaacgc gccggggccc tcaggcgccc agcatcagct tggcgccgag gccgatcatc
     6841 atcgccgcga cgagcccgtc caggacccgc caggacgacg gacgcgccag gacgccgctg
     6901 agcagccggg cgccgaaccc gagagcggcg aaccagcaca ggctggcgag gaccgctccg
     6961 aggccgaaca cccagcgccc ggagccccgg tcggccgcca ccgagccgag caggaacacg
     7021 gtgtccaggt agacgtgcgg attgagccac gtcatggcga ggcaggcgag cacggggccg
     7081 cgccgcgtcc cgttcggcgc atcccccgcg agcagggccg acggacgcag cgcgcgtcgt
     7141 gcggcgagga cgccgtagca gatcaggaac acgccgccga gcagcgcgac gaccgtcaga
     7201 gcggagggcc aggccacgac gacggccccc actccggcga cgccgagcgc gatgaggacc
     7261 gcgtcggaca gcgcgcatat cgccaccacc ggcaggacgg cgtcgcggcg caccccctgg
     7321 cgcaggacga gtgcgttctg ggcgccgatg gcgacgatga gggagagacc ggtggcgaat
     7381 ccggcggcgg cggtgctgag cgtgctggtc atggtgctga cgttacggag acgtgggggc
     7441 tgacgtacag ctaagaattc ttacgtatca ttagcgcttg tgatgccgga gcttcccctt
     7501 gatcaggtcc gcacgctgct ggccgtcgtc gacgagggca cgttcgacgc ggcggcgagt
     7561 gtgctgcacg tgacaccgtc cgcgatcagc cagcgcgtga aggcgctgga gcagcggacg
     7621 ggacgggtgc tgctgatgcg gaccaagccc gtacggccca cggagtccgg cgaggtggtg
     7681 gtgcgcttcg cccgccagct ggcccggctg gagcgcgacg cgatggcgtc gctcgggatg
     7741 tcgggcccgg gtgagccggc ccggctgtcg atcgcggtga acgcggactc cctggcgacc
     7801 tggttcctcc cggccctgcg ccgggtgccc gagacggcgg aggtctgcta cgagctgcgc
     7861 cgggaggacc aggaccacac gtccgccctg ctgcgggagg gacgggtgat ggcggcggtg
     7921 acctcgtcgc cggaggcggt gacgggctgc tcggtgcgcg gcctgggcgt gatgcgctac
     7981 ctgccggtgg ccggatccgg gttcgccgac cggtggttcg ggccgagggc gggcacgact
     8041 ccgcgcgatc tgctcgccgc cgcgccggtg gtgggcttcg accggcggga cgacctccag
     8101 gacaccttcg ttcgggggct cggcggtgac cgcgcgagcg gtcggcggca ctacgtgccc
     8161 acctccgagg ggttcgtggc ggcggtcgcg gccggcatgg gctggggcat ggtgcccgac
     8221 gtccaggcgg aggccctgct ggcggcgggc cgtctggtgg acctggctcc ggggcgcacc
     8281 gtcgacgtcc cgctgtactg gcagcagtgg aagctcgact cgccgtccct ggcggccgtc
     8341 gccggggcgg tgacggccga ggccgccgtg tcgttgcgcc ccggcggcgg gccgggcgcg
     8401 ggccccgcag gtcacgggag gtccggggcg tcctaggatg gcgtctctcc cggcccccgg
     8461 gcgcgtcggg cggtccgtcc gggccgcctc ggggaggggt gttcccgttc ccctgccgac
     8521 aactcccgga ggcgatctct tgcgcgtact tctggtggag gacgaggacg acctgcgcgc
     8581 ggtgatcgcc gcgggcctgc gcgaggcggg tttcgccgtg gactgcgcgc cggactggcc
     8641 ggacgccgat gtgatgctgc acgtcacggc gtacgactgc gtggtcctgg accgcatggt
     8701 gcccttcggc gacaccctgg tcccgctgga gcgccggcgg cggaccggct ggtccgtgcc
     8761 cgtcctgtgc ctcacggccc tggacacgct cgacgaccgg ctgttcgggc tggagagcgg
     8821 ggcggacgac tacctggcca agcccttcgc catgcgcgag ctggtgctgc gggtgcgcag
     8881 cctgacccgg cgggcgaccg agcgcctgcc cgccttcctg ggctgcgcgg acgtggtgat
     8941 ggacctggcc cggcgcgagg tgcggcgggg cggggtgctg ctgtccctga gcccgaagga
     9001 gtacgccgtg ctccagcagc tcctggtgaa cgcggagagc gtggtcacgc gcaccggtct
     9061 gctggagcac tgctgggacg agatggccga cccggtctcc aacgtggtgg acgccgtcat
     9121 cgcgggcgta cggcgcaagc tcggctcgcc ggggctggtg cacaccgtgc gcggtcaggg
     9181 gttcctgatg agcgcgggac gggggccgtc ctgatggcgg gccgccccga acgccgtcgg
     9241 cggtcgccgg ccagacggcg gctgcgccgg ctccagctgc ggctgaccgc cgcgtacacc
     9301 ctgatcaccg ccctcgggct gagctgcctg tgctggctgg tgatccgcac cgacgaccgg
     9361 tcccggcagg cgtcggagta cgacgagatg cgccgccggg cctccgtcgc ggcctcgctg
     9421 gtctactacg aggaggaccg gctccgcctc gacggtctcc aggacgacga ggcgaccacg
     9481 ggcaccccgc agatcctggt gctggaggcc ccgcccggcg ggccgcacgc cttcgtgttc
     9541 cgcggacggg tcagccggtt cgacgtcgcg gacgcgaccg cggaacgggt ggcgcgctcg
     9601 gcgatgcggg cggacgacgt ggtgcgctcg acggggcacg accggtccgg cgaccccgtc
     9661 tacctgctgg ccgtgccctt ccaccacgac gccaccgacg cgatggcggg cgcggccgtc
     9721 gcggtcggcg acccggaacg cggctcggcc gagcaccgca gtctgatcct gtccctggcc
     9781 gccggcggcg gggcgctcac ggccctcgcc gggctcaccg gccacgtgct gtcgggccgg
     9841 agcatgcgtc cggcctggca ggcgctggag cagcaggagc ggttgctggc cgacgcggcg
     9901 cacgagctgc ggacgccggt ggccgtgatg cgcggctcgg tggaggtggc cgagggcggc
     9961 gcgggcgggc tgggcgttca cctgccgcgc atccggcggg cggcggaccg gatggccgac
    10021 gtggtggaga acctgctgac ccgtgggcag ctggacgcgg ccgtggactc cgtccggccg
    10081 acgccgctgc gcctggacca gctcgtggag gaggtctgcg cggatctgcc ggacggcggg
    10141 caccggctgg agctgtgcct ggaggagtcc gtggtcgagg ccgacgcggg gctggtgcgg
    10201 gtggcggtgc gcaacctgct ggacaacgcc gcacggcacg gccggactcc gggcgcgccg
    10261 ggcggggctg atctgctggt gtccgtgctg gggccgtcgg tctcggtcgc cgaccggggc
    10321 cccggtgtgg agcccgggcg gctgccggag ctgatggagc gcttccgctc gccggggggc
    10381 ggtacgggca tcggcctgtc gctggtgcac cgggtcgcga ccgtccaccg cggaagcctc
    10441 accgtgcggg cccggcccgg cggtggcgcg gtcttcgtgc tgcggctcgc accgggccgg
    10501 tcgcggcgtc ggcgcgcggg cggacgcggc ggagtgcggg cgcggcgatg acgctcacga
    10561 ttcactcatg atcggccgtc catgatgcgg gggcaggttc tcagaccacg cacaggagta
    10621 cgcatgtcca agcacgccgt ccccccgttc ttccgccgca ggagcaccct cgcgggcgtc
    10681 gccgccggtg tcgcggtggc gaccggggcg ggcctgatgc tgtccaacgc cttcgcgacg
    10741 ggctccgccg tgaaagcggc ccccaaggtc gtcctcccca aggcgaacgc cgccttcgac
    10801 taccagatcg gcggtgccta cacgccgccc gccggggtca aggccgtctc ccgggaccgc
    10861 ggggacaagc cggcccgcgg cctgtacaac gtctgctacg tcaacgcctt ccagactcag
    10921 ccgggtgaga tggactggtg gcagaagaag aaccccgacc tgc
//
