LOCUS       NZ_ML123034            11194 bp    DNA     linear   CON 23-MAY-2024
DEFINITION  Streptomyces sp. ADI96-02 scaffold001, whole genome shotgun
            sequence.
ACCESSION   NZ_ML123034
VERSION     NZ_ML123034.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. ADI96-02
  ORGANISM  Streptomyces sp. ADI96-02
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 6907715)
  AUTHORS   Guerrero-Garzon,J.F., Zehl,M., Urban,E., Schneider,O., Ruckert,C.,
            Busche,T., Kalinowski,J. and Zotchev,S.B.
  TITLE     Streptomyces spp. from the marine sponge Antho dichothoma: analyses
            of secondary metabolite biosynthesis gene clusters and some of their
            products
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6907715)
  AUTHORS   Guerrero-Garzon,J.F., Zehl,M., Urban,E., Schneider,O., Ruckert,C.,
            Busche,T., Kalinowski,J. and Zotchev,S.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2018) CeBiTec, Bielefeld University, Sequenz 1,
            Bielefeld 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64.8x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003846185.1-RS_2024_05_23
            Annotation Date                   :: 05/23/2024 03:48:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,089
            CDSs (total)                      :: 6,032
            Genes (coding)                    :: 5,843
            CDSs (with protein)               :: 5,843
            Genes (RNA)                       :: 57
            tRNAs                             :: 54
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 189
            CDSs (without protein)            :: 189
            Pseudo Genes (ambiguous residues) :: 0 of 189
            Pseudo Genes (frameshifted)       :: 68 of 189
            Pseudo Genes (incomplete)         :: 155 of 189
            Pseudo Genes (internal stop)      :: 10 of 189
            Pseudo Genes (multiple problems)  :: 42 of 189
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:00:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 132205
            Orig. end                         :: 143399
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            ML123034.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Zotchev Lab, Department of
            Pharmacognosy, University of Vienna.
            Annotated using prokka v1.11 from http://www.vicbioinformatics.com.
FEATURES             Location/Qualifiers
     region          1..11194
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11194
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11194
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6194](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6194
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(470..1942)
                     /locus_tag="EES43_RS00530"
                     /old_locus_tag="EES43_00530"
     CDS             complement(470..1942)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020548159.1"
                     /locus_tag="EES43_RS00530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_00530"
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="WP_124266787.1"
                     /transl_table=11
                     /translation="MTDGKALPRPVVDGYPGRPSVRAGEVLRLHVSTRAERFRADFYRC
                     GPRPRLMGRTRGRGAYAEPGSHDADWNWPVHDVAIPADWPSGVYIAVLGPDAESPDITS
                     PDTTSPDITSPDEAGPDARFRDARRPVDAAALDARHSRLLFVVTAREPGHRARILYKVP
                     LFTYHAYNRSGGASLYGALPGTEDRRTVTLRRPGGGIGGPVKGRPDAHDPATPRQTFAH
                     WDAPFIAWLEAAGFTADYCTDLDLDENPELAHPYRLLLSAGHDEYWSGRVRRHTSSFRD
                     RGGNIAIFGANTCWWRVTPDATGTAVSCAKYPPGVPQGADTDRMRGAPDHWWEEEPENR
                     LIGVSYRNGGGHWDGLRERLGFTVQHTGHPVFAGTGLRDGDVLGAEHHLVGYECDGAAH
                     RRDAHGRAVATGEDGTPEDFTILGVADLPAAPGSGWHTAARENDDPGRAATFGLYTRGG
                     TVFNAATTDWPRLLRLDPRVRTITRNVITLLT"
     gene            complement(1939..3078)
                     /locus_tag="EES43_RS00535"
                     /old_locus_tag="EES43_00535"
     CDS             complement(1939..3078)
                     /EC_number="2.4.-.-"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /locus_tag="EES43_RS00535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_00535"
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="WP_124266788.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 2.7e-16,
                     bitscore: 58.1, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRIAYLHGGGIPSPFANGVHVMRMCDAFAAAGHQVTLYSIPGTDE
                     EPYAYYGVRHRFPIRLVACPDYTPSGYRKRAEAVRALVADGPAPDLVYGRDPYALSALA
                     DLAPLVYEIHQLREDLSPAGTEEALLAAPRLVRVVAITHALARDVRDKYAALGPLPIVV
                     APDCADPPGPAPADPLPLPGRPDALRVGYVGHLYDGRGIGLVLELAERFPALDFHLVGG
                     TDEDRTRWQQVCRSPHAHFHGHRPPSELSGYYARFDVVLAPYERKVYTWGRLGETGRWA
                     SPMKVFEYMSHGCPMIASDLPVLREVLQDRVNCLLRPPEDADAWAGALNELAADPALRR
                     GLGAEGRRQVLERYTWRRRADVVLAGLGAVETVRPEPAA"
     misc_feature    complement(3306..3326)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 18.84"
                     /tool="antismash"
     gene            4033..5004
                     /locus_tag="EES43_RS30425"
                     /old_locus_tag="EES43_00540"
     CDS             4033..5004
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04267.2"
                     /locus_tag="EES43_RS30425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_00540"
                     /product="HEXXH motif-containing putative peptide
                     modification protein"
                     /protein_id="WP_164493085.1"
                     /transl_table=11
                     /translation="MRVEPDAREGAEERSALALMARTALERVGLEVSPREAAHPAVVEA
                     AHIAQYVLRTGPPVPERLTRLTGMLDRARSDGTGTRPSALPWPTGLAAPGPHLERSVAR
                     ALKSVPDRGAGAGAPRGSSVAGWRAPEEEAARSAVALLESAWPGVAGELSTVLVQIALL
                     DGPGIDGFTDFTVHGAVFVRRDRLAPDADGLPGSVRFAEALVHEGAHTRCNAASVADRP
                     FLRPLEESGPMVATPLRLDPRPLTGLFQQMVVLARSVLLYRRLLAREAGGVGAAPAVRA
                     RHDRLAHSAAEAVRTMTLHRDTLTEHGAAMLEDAAEAARVRV"
     gene            5001..6194
                     /locus_tag="EES43_RS00540"
                     /old_locus_tag="EES43_00545"
     CDS             5001..6194
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /locus_tag="EES43_RS00540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_00545"
                     /product="YcaO-like family protein"
                     /protein_id="WP_164493086.1"
                     /sec_met_domain="YcaO (E-value: 1.6e-35, bitscore: 121.8,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAASAWAPQVFAPYASWPRVVLARVAARSPQFDAVCAAGGRPVI
                     VGSAAGHDSERVAASARGELLERVGNVLAGRAAESDAAVVASHRELRRKGIPAVDPAEL
                     APDGGAAALDDAREARRLWVRGRFLRSGADVHVPAGAVFLHHRAPSGCDPGPGAGSTGL
                     AAHPDPEAATRHAAWEVLERDLVRRSWYGLLPGPRVMASARLPEPLAELLDGNAISATA
                     LDLPAPAGSRCVAVALHAADGTRQTFGARCGPEAEGVAPLAEKAAYEALMVRWSMKTAV
                     AEQTWARWRGESPPGSAVEHALWAFHRQDSLRLWTARAPRSRGRRETAPAGRHRDGTAV
                     LAEHTGRDPVVVDTTCGPANDLGSTVVRIVAPGARALPTGPDTERGLPIAPGPHPFG"
     gene            6390..7145
                     /locus_tag="EES43_RS00545"
                     /old_locus_tag="EES43_00550"
     CDS             6390..7145
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1248: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /locus_tag="EES43_RS00545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_00550"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_124266789.1"
                     /transl_table=11
                     /translation="MTTYHERPAVPAGRYSDDHAADYDRWFGKPGITAATVDTLALLAG
                     EGRVLELGVGTGRIALPLARRGFEVHGVEASQAMAARLRAKPGGEGVRITLGDFAEVPV
                     EGVFSLILVVGGTLFELPSHEDLQRCLTAAAEHLEPEGVLVLDAHVPEALAVAAASGVP
                     EVVSESDDHLILCHRRIDPSTATYHSHYLVHEADRTHHLRVRFHYASPGELDLMAERAG
                     LRLRTRLGSWAGGPFTRGSAYHVSVYERR"
     gene            7215..>7624
                     /locus_tag="EES43_RS30430"
                     /pseudo=""
     CDS             7215..>7624
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EES43_RS30430"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /product="cytochrome P450"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTDIDADGPVPTADFPFPHHDGLGPLPELAALRRDAPCARIVLPS
                     GDPAWLVTRYADVRTVLADPRFSRRRATRGAGPRMARVPTLRDSILAADPPEHSRLRKL
                     VAPAFTARRAEAMRSGVARLVDELLDRLAAHE"
     assembly_gap    7625..7649
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7650..8463
                     /locus_tag="EES43_RS00550"
                     /old_locus_tag="EES43_00555"
     CDS             <7652..8463
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /locus_tag="EES43_RS00550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_00555"
                     /product="cytochrome P450"
                     /protein_id="WP_164493088.1"
                     /sec_met_domain="p450 (E-value: 2.5e-13, bitscore: 48.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="RLAAHERPADLVPLLARPLPLAVICDLLGVPREDGDRLDAWCDAL
                     RNLTAAPDAEVTAAVGEMTGYLTGLVAAKRRRGGDDVLSTLIAARDEGDRLSQDELVSF
                     ALVLLAGGYGTTADRLAGSVHLLLDDPARYGQLVREPATIPRAVEELLRYAQTNVQANL
                     RVATEELRIGGVRIAEGEAVMAINSSANHDENVFDAPDLLDLARERNPHLGFGHGVHHC
                     VGAQIARVQLQEALAGLVRRFPGLRAAGPTEWKTGLKTRGPRSLPVSW"
     gene            8471..8743
                     /locus_tag="EES43_RS00555"
                     /old_locus_tag="EES43_00560"
     CDS             8471..8743
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014628745.1"
                     /locus_tag="EES43_RS00555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_00560"
                     /product="ferredoxin"
                     /protein_id="WP_124266791.1"
                     /transl_table=11
                     /translation="MPREGEPRTARAAGPEAAARVFVDPALCIGSGICLATAPRHFEPG
                     GDNRSRARPDAPASDESAEDAAALCPVEAIGLVAGPPAPGPPPGP"
     gene            8795..9616
                     /locus_tag="EES43_RS00560"
                     /old_locus_tag="EES43_00565"
     CDS             8795..9616
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006345543.1"
                     /locus_tag="EES43_RS00560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_00565"
                     /product="aldo/keto reductase"
                     /protein_id="WP_124266792.1"
                     /transl_table=11
                     /translation="MRTLTLGNGVSVPRLGLGVWPLTDAQAYAAVSHALACGYRHFDTA
                     RVYDNEAGVGRAIRDAGIPRQDVFLTTKLWNADQGYDAALRAFDAALQRLGTDYVDLYL
                     IHWPVPEQDLYVESYRALEQLHRDGRARAIGVSNFTAATLERLLSCTDVVPALNQIELH
                     PYFQQRRMRESCALRSIATGAWSPLAQGGALLDEPVLLGIAGRHGKTAAQVVIRWHLQL
                     GHVVIPRSVNPSRIEENIDVFDFRLGDEEMRAIAALDDDGGRIGPDPDGPG"
     gene            complement(9935..10540)
                     /locus_tag="EES43_RS00565"
                     /old_locus_tag="EES43_00575"
     CDS             complement(9935..10540)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018839273.1"
                     /locus_tag="EES43_RS00565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EES43_00575"
                     /product="response regulator transcription factor"
                     /protein_id="WP_124266793.1"
                     /transl_table=11
                     /translation="MIRVLLADDEHLIRGALASLLGLEDDIDVVAQAGTGDEALAMAVA
                     HRPDVAVLDLRMPVLDGVVVAERLRDTLPGCASMIVTSHAGPGALKRALSVGVRGFVPK
                     TVSARELARIIRTVHTGSRYIDQELAADAIAAGESPLTPREAELLAYAEDGAPVSDIAS
                     RASLSAGTVRNYLSAAAAKLGAENRHVAARIARERGWL"
     misc_feature    complement(10959..10977)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.57"
                     /tool="antismash"
ORIGIN
        1 cgtcctcgac gtaggagtcg ccggtgatca ggcggctgac ccgttccttg tccacgtcgt
       61 ggccgacgac ctggtatccg ctcgcggcgg cccgtacggc cagggacagt ccgacgtagc
      121 cctgtccgac gacgaccacg cggccgggga ccgggcgggc ctgcgggtcg ggcgtcgcgg
      181 tgccgcgcgg caccgccccg tccggggcgg ccgggttcac ctcgcctccc cgcatccgtg
      241 ccgtcgtggg ccggccgccc cgggcgccgg tccgttcggg gcgtggaggc gtgcggtgcg
      301 ggtcctgccg cgcgtggtcg ccgcgcgagc gctgcgaccg gccgtgccgg gccgtacggg
      361 catgagtggt cccctccggg cgggttcccg ccctgtgcga gtgggcgcag gacgctcgcc
      421 ctgcgtgaaa ccggtgtgcg cggtgcggaa cggccgcggg cggcggacgt caggtgagca
      481 gggtgatcac gttgcgggtg atggtccgca cgcgggggtc cagacgcagg aggcgcggcc
      541 agtcggtggt ggcggcgttg aagacggtgc cgccgcgggt gtagagcccg aaggtggcgg
      601 cccgccccgg atcgtcgttc tcgcgtgcgg cggtgtgcca ccccgatccg ggagccgcgg
      661 ggaggtccgc gacgcccagg atggtgaagt cctcgggggt tccgtcctcg cccgtcgcca
      721 ccgcccggcc gtgcgcatcg cgccggtgcg ccgctccgtc gcattcgtag cccacgaggt
      781 ggtgctcggc gcccaggacg tcgccgtcgc gcaggccggt gcccgcgaag accgggtgtc
      841 cggtgtgctg gacggtgaag cccagccgct cgcggagtcc gtcccagtgt ccgccgccgt
      901 tgcggtagct gacgccgatg agccggttct ccggctcctc ctcccaccag tggtcggggg
      961 cgccccgcat ccggtcggtg tcggctccct ggggcactcc gggcgggtac ttggcacagc
     1021 tgacggcggt gcccgtcgcg tcgggcgtca cgcgccacca gcaggtgttg gccccgaaga
     1081 tcgcgatgtt gccgccccgg tcgcggaagg aggaggtgtg ccggcggacc cgcccgctcc
     1141 agtactcgtc gtgccccgcg gacagcagca gccggtaggg gtgggcgagt tcggggttct
     1201 cgtcgaggtc gaggtcggtg cagtagtcgg cggtgaatcc cgccgcctcc agccacgcga
     1261 tgaacggcgc gtcccagtgg gcgaaggtct ggcggggcgt tgccgggtcg tgcgcgtcgg
     1321 gcctgccctt caccgggccg ccgatcccgc cgccgggacg ccgcagggtg acggtgcgac
     1381 ggtcctcggt cccggggaga gcgccgtaca ggctggctcc gccgctccgg ttgtaggcgt
     1441 ggtaggtgaa gagcggcacc ttgtacagga tgcgggcccg gtggccgggt tcacgggccg
     1501 tcaccacgaa cagcagccgc gagtggcggg cgtccagggc ggctgcgtcg acgggccgcc
     1561 gggcgtcgcg gaaccgggcg tccgggcctg cctcgtcggg gctcgtgatg tccgggctcg
     1621 tggtgtcggg gctcgtgatg tccgggctct cggcgtccgg gccgagcacg gcgatgtaga
     1681 cgcccgaggg ccagtccgcg gggatcgcga cgtcgtggac gggccagttc cagtccgcgt
     1741 cgtggctgcc cggctccgcg tacgcacccc gtcctcgcgt acgtcccatg aggcgcgggc
     1801 gcgggccgca gcggtagaag tcggcccgga agcgttcggc acgcgtcgag acgtggagcc
     1861 gcaggacctc cccggcccgc acgctcggcc gcccggggta gccgtccacc accggccggg
     1921 gcagggcctt cccgtccgtc atgccgcggg ctccgggcgg acggtctcca cggcgccgag
     1981 gccggcgagg acgacgtcgg cccgccgtcg ccaggtgtag cgctccagga cctgccgccg
     2041 cccctcggcg ccgagccccc ggcgcagggc cgggtcggcg gccagttcgt tcagcgctcc
     2101 cgcccaggcg tccgcgtcct cgggcgggcg gaggaggcag ttgacccggt cctggaggac
     2161 ttcccgcaga accggcagat cggaggcgat catggggcat ccgtgggaca tgtactcgaa
     2221 caccttcatg ggcgaggccc agcgccccgt ctcccccagc ctcccccacg tgtacacctt
     2281 gcgctcgtaa ggggcgagga cgacgtcgaa gcgggcgtag taaccggaca gttcggacgg
     2341 cggccggtgg ccgtggaagt gcgcgtgcgg ggaacggcac acctgctgcc agcgggtgcg
     2401 gtcctcgtcc gtgccgccga ccagatggaa gtcgagcgcc ggaaaccgtt ccgccagttc
     2461 caggacgagg ccgatgccgc gcccgtcgta gaggtgaccg acgtagccga cgcgcagggc
     2521 gtccgggcgg cccggcagcg gcagggggtc ggccggggcg gggccgggcg ggtccgcgca
     2581 gtccggcgcg accacgatgg gcagcgggcc cagggccgcg tacttgtccc gtacgtcgcg
     2641 tgcgagggcg tgggtgatcg cgacgacgcg gaccagccgg ggcgcggcga gaagcgcttc
     2701 ctcggtcccg gccggggaca ggtcctcgcg cagttggtgg atctcgtaca cgaggggcgc
     2761 gaggtcggcg agggcggaca gggcgtaggg gtcgcggccg tagacgaggt caggggccgg
     2821 gccgtccgcc accagcgccc ggacggcctc ggcgcgcttc cggtagccgg agggcgtgta
     2881 gtcggggcag gcgacgaggc ggatcgggaa gcggtggcgc acgccgtagt aggcgtacgg
     2941 ctcctcgtcc gtgccgggga tggagtacag ggtcacctgg tgcccggcgg cggcgaacgc
     3001 gtcgcacatc cgcatgacgt ggacgccgtt ggcgaagggg gaggggatgc cgcccccgtg
     3061 caggtacgcg atgcgcatgc ggcctagccc cgtgcggccg ttgcggcggg cgcggtccgg
     3121 cgctcggcgc accgggggcg gtgaggcgtg gtgtgcgtcg gatccacggg cgactcctca
     3181 ggacggcgcg acgacgggcg gtgccggtcc ggtgacgcgg cagcggtccg gccgcccgga
     3241 ggggcggagc gcgcggtgag acggggcgag gcggtagggc ggggcggatc gggtcggcgg
     3301 cggagcaccc cgccggagcg gtgtttcgcc tggcctgcgc cgggacgggg cagatgacga
     3361 agcatcatgc tgcgtggtgc cgggcttttc caagagtggt ctttcggccg tttcgacagt
     3421 cctgtttgcg ccgctctccg ccgcacgggg cccggctcgt ggcaccggtg cgcgaccacc
     3481 ggcgcacccc tccggcgtcg acggggcagg ccctcccggc accttccgga cagaaaagtg
     3541 tccgagacgg aaccgatgac acagcgggac atgatgtgcc ccggcgcgtc ctcccgaacc
     3601 ggcggcgggc ggtccacggg gcacggtgga ccggaccggc ggaccggaac ccgcaggcca
     3661 cggagcgcgc ggcgccggcc gccgaccgct cggagcgccg gacggcgaca cggatgccgc
     3721 ggcacgccca ccgcccggac gggccgccgc gcgtacgtcg cggggaacgg gccctacctt
     3781 gtgctcggtt ccggcccgtg cctaccgtgg ctgcgccgtg actccccgtc aacgtcggag
     3841 gaggcggtca tcgaagggag atcgatatga acgaggagat cgagcagatt cccgaggaag
     3901 aacttcccga actggcgctg atggtcgaca ttcccagctg acgccgcgcg cccggggcgg
     3961 cggacctcac cggtccgccg cccgcccgct cactgctccc ccgctcctac gtcagcacgg
     4021 aggtcacgca cggtgcgagt ggaaccggac gcgcgcgagg gcgccgagga gcgcagcgca
     4081 ctggcgctca tggcgcgcac agctctcgaa cgggtaggtc tggaagtctc cccgcgggag
     4141 gccgcgcacc ccgcggtggt cgaggcggcg cacatcgccc agtacgtgct gcggaccggg
     4201 ccgcccgtgc ccgaacgtct cacccggctc accggcatgc tcgaccgcgc ccgctcggac
     4261 ggaacgggca cccgtccgtc ggcacttccg tggccgaccg gactcgccgc accgggaccg
     4321 catctggagc gcagtgtcgc ccgcgcgctg aagtccgtac cggatcgagg agccggggcc
     4381 ggcgcaccgc gcggctcctc ggtggccggg tggcgcgcac ccgaggagga ggccgcccgc
     4441 agcgcggtcg ccctgctgga gtcggcctgg ccgggcgtgg cgggcgagtt gagcaccgtg
     4501 ctcgtgcaga tcgccctgct ggacgggccg ggcatcgacg gcttcacgga cttcaccgtc
     4561 cacggcgccg tcttcgtgcg gcgcgaccgt ctcgctccgg acgccgacgg gctgccgggt
     4621 tccgtccggt tcgccgaagc gctcgtccac gagggcgccc acacgcggtg caacgccgcg
     4681 tccgtggcgg accggccctt cctgcggccc ctggaggaga gcggcccgat ggtcgccacg
     4741 cctctccggc tcgatcccag gccgctcacc ggactcttcc agcagatggt ggtcctggca
     4801 cgcagcgtcc tgctgtaccg gaggctgctc gcccgggaag cgggcggcgt cggagcggcc
     4861 ccggccgtgc gggcgcgcca cgaccggctc gcccactcgg ccgcggaggc ggtccggacc
     4921 atgacgctcc accgcgacac gctcaccgag cacggggcgg cgatgctgga ggacgccgcc
     4981 gaggcggcgc gggtgcgggt gtgacggccg cgtccgcctg ggcgccccag gtcttcgccc
     5041 cgtacgcctc ctggccccgg gtcgtcctcg cccgcgtggc ggcgcgctcg ccgcagttcg
     5101 acgccgtctg cgccgcgggg ggccgtccgg tgatcgtggg cagcgcggcc gggcacgact
     5161 cggaacgcgt cgcggcgagc gctcgcggcg aactcctcga acgcgtcggc aacgtgctcg
     5221 cgggccgcgc cgcggagtcc gacgcggcag tggtcgcctc ccaccgggag ctgcgccgca
     5281 aggggattcc cgcggtcgac cccgccgaac tcgcgccgga cggcggggcg gcggcgctcg
     5341 acgacgcccg cgaggcccgc aggctgtggg tgcgcgggcg cttcctgcgc tccggggcgg
     5401 atgtgcacgt gcccgccggg gcggtcttcc tgcaccaccg ggctccgtcc ggatgcgatc
     5461 ccggtccggg cgcgggctcc accggcctgg ccgcccatcc cgatccggag gcggccacgc
     5521 ggcacgccgc ctgggaggtt ctggagcgcg acctcgtccg caggagctgg tacggcctgc
     5581 ttccgggccc gcgcgtgatg gcctcggcga ggttgccgga gccactggcc gaactcctgg
     5641 acggcaacgc gatctcggcc accgcgctcg atctgcccgc gcccgccgga tcgcgctgcg
     5701 tcgcggtggc cctgcacgcg gccgacggaa cgcggcagac gttcggagcc cgctgcggcc
     5761 cggaggcgga gggcgtggcc ccgctggccg agaaggccgc ctacgaggcg ctgatggtgc
     5821 gctggagcat gaagacggcg gtggcggagc agacgtgggc gcgctggcgg ggcgagagtc
     5881 caccgggctc cgccgtcgaa cacgcgctgt gggcctttca ccggcaggac agcctccggc
     5941 tgtggacggc acgcgccccg cggagccgcg gccggcgcga aacggctccc gccggtcggc
     6001 accgggacgg gacggccgtg ctcgccgagc acaccggccg ggacccggtc gtcgtggaca
     6061 ccacctgcgg accggcgaac gacctggggt cgaccgtcgt acggatcgtg gctccgggag
     6121 cccgtgccct gccgaccggt ccggacacgg aacgcggcct tccgatcgcg ccgggccctc
     6181 atcccttcgg atgacccgcc cacctcatcc cgcccgtcct cacccggccc gagcccgatc
     6241 ccgacccgtc cgcccgcccg tccgttcacc tgctcgtccg ttcgtccgct catctgccca
     6301 ccaccaccgc cgccagtcag ccaccacatc cgcgtcgacc gccgctcggt acggcgaccg
     6361 gaccgacccg caggccgagg agccccggca tgaccaccta ccacgagcga ccggccgtcc
     6421 cggcgggccg gtactccgac gaccacgcgg ccgactacga ccggtggttc ggaaagccgg
     6481 gcatcaccgc ggcgaccgtg gacaccctcg cgctcctcgc cggcgagggc cgggtgctcg
     6541 aactcggtgt cggcaccggc aggatcgcgc tgcccctggc ccggcgcggc ttcgaggtcc
     6601 acggcgtcga ggcgtcgcag gcgatggcgg ccaggctccg ggcgaaaccc ggcggggagg
     6661 gcgtccggat cactctgggc gacttcgccg aggtgcccgt cgagggcgtg ttctccctca
     6721 tcctggtcgt gggcggcaca ctgttcgagc tcccctccca cgaggacctt cagcggtgcc
     6781 tgaccgccgc ggcggagcac ctcgaaccgg agggcgtcct cgttctcgac gcccatgtgc
     6841 ccgaggcgct ggccgtcgcc gccgcgtcgg gggtgcccga ggtggtctcc gagtccgacg
     6901 accatctgat cctctgccac cgcaggatcg acccgtccac cgccacctac cactcccact
     6961 acctcgtcca cgaggcggac cgcacccacc acctgcgcgt ccggttccac tacgcgtcac
     7021 cgggcgagct ggacctgatg gccgaacggg cggggctccg gctgcgtacg cgcctggggt
     7081 cctgggccgg ggggccgttc acccgtggca gcgcctacca cgtctccgtc tacgaacgcc
     7141 gctgagacct cccctgccgt acgcggtcgt cgacgcccgg cgtccgcacc gccgagaacc
     7201 ctcccggagc agtcgtgaca gacatcgacg cagatggccc ggtccccacc gcggactttc
     7261 ccttcccgca ccacgacggg ctgggtccgc tgccggagct ggcggccctg cgccgcgacg
     7321 ccccgtgcgc ccggatcgtc ctgcccagcg gcgacccggc atggctggtc acccgctacg
     7381 ccgatgtgcg caccgtgctg gccgatccgc gcttcagccg taggcgcgcc acccggggcg
     7441 cggggccccg tatggcgcgt gtcccgaccc tgcgggactc gatcctggcg gcggacccgc
     7501 ccgaacacag ccggctgcgc aaactggtcg ccccggcctt caccgcccgg cgcgccgagg
     7561 ccatgcggtc cggtgtggcc cggctcgtgg acgagctgct ggaccggctc gccgcgcacg
     7621 agcgnnnnnn nnnnnnnnnn nnnnnnnnnc cggctcgccg cgcacgagcg gcccgccgat
     7681 ctcgttccgc tgctcgcccg gccgctcccg ctggccgtga tctgcgacct gctcggtgtc
     7741 ccccgggagg acggcgatcg gctcgacgcc tggtgcgacg ccctgcgcaa tctgacggcc
     7801 gccccggacg cggaggtcac ggccgccgtc ggggagatga ccggttacct gaccgggctc
     7861 gtcgcggcca agcgccggcg aggcggcgac gacgtgctga gcaccttgat cgccgcacgg
     7921 gacgagggcg accggctcag ccaggacgag ctggtctcct tcgctctcgt gctgctagcc
     7981 gggggctacg gcaccacggc cgaccggctc gccggctcgg tgcacctgct gctggacgac
     8041 cccgcgcggt acggacagct ggtccgcgaa ccggcgacga tcccccgcgc cgtcgaggaa
     8101 ctgctgcgct acgcccagac caacgtgcag gccaacctgc gggtcgccac cgaggagctc
     8161 cggatcggcg gcgtccggat cgccgagggc gaggccgtga tggcgatcaa ctcgtcggcc
     8221 aaccacgacg agaacgtctt cgacgccccc gatctcctcg acctggcccg cgaacgcaat
     8281 ccgcatctcg ggttcggcca cggcgtccac cactgcgtcg gcgcccagat cgcgcgcgtc
     8341 cagctccagg aagccctggc cgggctcgta cgccggttcc cggggctgcg cgccgccggg
     8401 ccgaccgagt ggaagaccgg tctgaagacg cggggacccc gcagcctgcc cgtgagctgg
     8461 tgaggcggtc gtgccccgtg aaggcgagcc ccgtacggct cgtgcggcag gcccggaggc
     8521 cgcggcccgg gtcttcgtcg acccggccct gtgcatcggg tccggcatct gcctcgccac
     8581 cgcgccccgg cacttcgaac ccggcgggga caaccgctcg cgggcacggc ccgacgctcc
     8641 ggcgtcggac gagtcggccg aggacgcggc ggccctgtgc ccggtggaag ccatcggcct
     8701 cgtcgccggg ccgcccgcgc caggcccgcc gccgggcccc tgacagccgg cggctcgacg
     8761 acgcccccgt acccgtaccc ggaaggatcg ccgcatgcgg acactgaccc tcggcaacgg
     8821 ggtgtccgtc ccccggctcg gcctcggcgt ctggccgctg accgacgccc aggcgtacgc
     8881 cgccgtctcg cacgccctgg cctgcggcta ccggcacttc gacaccgccc gggtgtacga
     8941 caacgaggca ggggtcggcc gcgcgatccg ggatgccggg atcccgcggc aggacgtctt
     9001 cctcaccacc aagctctgga acgccgacca gggctacgac gccgcactgc gggccttcga
     9061 cgccgccctc cagcgcctgg gcacggacta tgtcgacctc tacctgatcc actggcccgt
     9121 tccggaacag gacctgtacg tcgagtccta ccgggcgctc gaacagctcc accgcgacgg
     9181 acgcgcacgg gcgatcggcg tctccaactt caccgccgcg accctggaac gcctgctgtc
     9241 gtgtaccgat gtcgttccgg ccctcaacca gatcgagctc cacccctact tccagcagcg
     9301 ccgcatgcgg gagtcgtgcg ccctcaggtc catcgcgacc ggcgcgtgga gcccgctggc
     9361 ccagggcggc gcgctgctcg acgagcccgt gctgctgggc atcgccgggc ggcacgggaa
     9421 gacggcggcc caggtcgtca tccgctggca cctccagctc ggccatgtgg tgatcccccg
     9481 gtccgtgaac ccgtcacgga tcgaggagaa catcgacgtc ttcgacttcc ggctcggcga
     9541 cgaggagatg cgcgcgatcg ccgccctcga cgacgacggc ggccggatcg gccctgatcc
     9601 ggacggaccg ggttgatgtg gcgagtctgg tcgatgcccg gggggcgttc gatgtggggg
     9661 ccacatggcg tgcgaccccc ggggcctgtc gaggggtggc tcggaagcgg ttcgaggggc
     9721 gcggcagcca gggcttgtgg gtcccggagg tgcggggcgc gaccggtgcg cggacggtgt
     9781 ccttcaccgc gagcttcccg gtgatcagag aggacttctc cagcgcgtcc gcacacgcgt
     9841 tcccgctcag gaggtagacc ccgtggccct ccccgtcctt gacggtcacc accttcgagc
     9901 ggcacctccc gggcgaaggg gcggaagcgg gctctcagag ccatccgcgt tcgcgcgcga
     9961 tgcgggcggc gacatggcgg ttctcggcgc cgagcttcgc cgcggcggcc gagaggtagt
    10021 tgcgcacggt cccggcggac agcgacgcgc gggacgcgat gtcgctgacc ggcgcaccgt
    10081 cctccgcgta cgccagaagc tccgcctcgc ggggcgtcag gggcgactcg cccgcggcga
    10141 tggcgtcggc cgcgagttcc tggtcgatgt agcggctgcc cgtgtgcacg gtgcggatga
    10201 tccgcgccag ttcccgcgcg gacaccgtct tgggtacgaa gccgcgcacc ccgacgctca
    10261 gggcacgttt cagcgccccc ggaccggcgt ggctggtgac gatcatcgag gcgcagccgg
    10321 gcagggtgtc gcgcagccgc tccgcgacca cgacaccgtc caggacgggc atccggaggt
    10381 cgaggacggc gacgtcgggg cggtgcgcca cggccatggc cagcgcctcg tcgcccgttc
    10441 cggcctgggc gaccacgtcg atgtcgtcct ccagtccgag gagcgaggcg agggcaccgc
    10501 ggatcaggtg ctcgtcgtcc gccagcagga ccctgatcat gcgtctgcct cccatggaat
    10561 gcgcacggtc aggtggaaga ccccgcccgg ccgccgttcg gtgaccagcg tgccgttcag
    10621 cgcggacagg cgttccctca ggcccggcag cccggtgccg ggcccctccg ccgcctcggg
    10681 cgccccgtcg ttctccacca ccagcagcac cgcgcccggc ccggccgtgc gcagcgacac
    10741 gttgcagcgt gaggctccct gggcgtggcg gagcacgttc gtcgtcgcct cgcgcaccgc
    10801 ccagccgagt gcggcctgga tgtcggaggg cagccgggcg gcggggccct cctcgatgcg
    10861 gcaggtgatg cccgccgctt ccagcaccga acgggcgccg gccagttcgg tgtgcagatc
    10921 ggcggtgcgg tagccgcgca ccaccgaccg gatctcccgc tgcgagtcct gcgcgatgcg
    10981 ctgcacctcc ctcatctgct cctcggcctc cggcctgcca cgccgcacca gttgcaccgc
    11041 gagttcgctc ttgagcgcga cgaccgcgag attgcggccc agcacgtcgt gcaggtcgcg
    11101 gccgaagcgc agcctctcct cggccacggc gagccgggtc tgcgtctgcc gtgccgcggc
    11161 cagttcctcg acgagccgga cgctccagcc gctg
//
