LOCUS       NZ_RIAS01000038         2271 bp    DNA     linear   CON 13-MAY-2024
DEFINITION  Paenibacillus amylolyticus strain 27C64
            NODE_38_length_2271_cov_19.6855_ID_75, whole genome shotgun
            sequence.
ACCESSION   NZ_RIAS01000038
VERSION     NZ_RIAS01000038.1
KEYWORDS    WGS; RefSeq.
SOURCE      Paenibacillus amylolyticus
  ORGANISM  Paenibacillus amylolyticus
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Keggi,C. and Doran-Peterson,J.
  TITLE     Paenibacillus amylolyticus 27C64 has a diverse set of
            carbohydrate-active enzymes and complete pectin deconstruction
            system
  JOURNAL   J Ind Microbiol Biotechnol 46 (1), 1-11 (2019)
   PUBMED   30377865
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Keggi,C. and Doran-Peterson,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2018) Microbiology, University of Georgia, 120
            Cedar St, Athens, GA 30602, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 23-MAR-2015
            Assembly Method                   :: SPAdes v. MARCH-2015
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 93.0x
            Sequencing Technology             :: Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003719335.1-RS_2024_05_13
            Annotation Date                   :: 05/13/2024 02:42:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,967
            CDSs (total)                      :: 5,826
            Genes (coding)                    :: 5,778
            CDSs (with protein)               :: 5,778
            Genes (RNA)                       :: 141
            rRNAs                             :: 5, 10, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 10, 9 (16S, 23S)
            tRNAs                             :: 113
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 8 of 48
            Pseudo Genes (incomplete)         :: 39 of 48
            Pseudo Genes (internal stop)      :: 7 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:46:40
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2271
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            RIAS01000038.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     CDS             complement(<1..2269)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (111-180). E-value: 9.6e-24. Score:
                     75.4. Matches aSDomain: nrpspksdomains_EC604_RS29285_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (195-480). E-value:
                     1.6e-107. Score: 350.9. Matches aSDomain:
                     nrpspksdomains_EC604_RS29285_Condensation_LCL.1"
                     /codon_start=2
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005544418.1"
                     /locus_tag="EC604_RS29285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EC604_29090"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_151458141.1"
                     /sec_met_domain="PP-binding (E-value: 3.5e-17, bitscore:
                     49.0, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 3e-87, bitscore:
                     278.5, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 7.5e-33, bitscore:
                     99.7, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRGYRIETGEVEAKLLQVKGVREAVALVRTDAEGQKVLCAYYTPD
                     AGTDLAVNNLRGALAQDLPGYMIPSYFVEMERLPLTPNGKIDRKALPAPEGEARSGTEY
                     VAPRNELERKLASIWQEVLGLTKEIGVYENFFDIGGHSLRATTLVSKIHKELNVDLPLR
                     EVFRHSMIENMAVAISRLDEQKFVAIPLADKREVYPQSFAQKRLFILNQLEGAELSYNM
                     PEAMVLEGALDRARFEDAFHKLVERHEMLRTGFEMVDGEASQRIYQELDFSVEFYSADE
                     QEVEEIVRRFVRPFDLGKPPLLRVGLVQLTPERHILMYDMHHIISDGVSMEIFVEEFVR
                     LYGGEQLEPLRIQYKDYSVWLHSQEQKERHQHQEAYWLNMFQGEIPVLEMPTDYPRPAV
                     QSYEGHTLEFFLEASKTDALKQLASETGTTLFMVLLAAYNVLLHKYSGQEDMVVGTPIA
                     GRNHGDVQPLIGIFLNTLAIRSYPSSEKTFLSYLNEIKETTLHAFEHQNYPFEELVDKV
                     QVDRDLSRNPLFDTLFTMQNTENDEFELEGLRLTSYPSAMDTAKFDISLDVGEENGGLD
                     YSFEYATALYKRETIERLAKHYEQLLVTIVSRPDAKIAELSLLRTEEKEMLDSFNPAQL
                     EVEVTLAAAFHRLFEEQVQRAPESEAVVYENDRLTYAELNERANRLASTLRASGIGRES
                     IVGIFAERSVELLVAVMAVWKAGGAYVPLDPDYPADRVRFMLEDSGAKVLLTQTPLRER
                     AEA"
     source          1..2271
                     /collection_date="2015-01-01"
                     /db_xref="taxon:1451"
                     /mol_type="genomic DNA"
                     /organism="Paenibacillus amylolyticus"
                     /strain="27C64"
                     /submitter_seqid="NODE_38_length_2271_cov_19.6855_ID_75"
     gene            complement(<1..>2271)
                     /locus_tag="EC604_RS29285"
                     /old_locus_tag="EC604_29090"
     region          1..2271
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..2271
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2271
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:2269](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..2269)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS_motif       complement(97..138)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_EC604_RS29285_0006"
                     /evalue="1.80E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="EC604_RS29285"
                     /protein_end="724"
                     /protein_start="710"
                     /score="20.9"
                     /tool="antismash"
                     /translation="MAVWKAGGAYVPLD"
     CDS_motif       complement(664..783)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_EC604_RS29285_0005"
                     /evalue="3.90E-21"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="EC604_RS29285"
                     /protein_end="535"
                     /protein_start="495"
                     /score="67.5"
                     /tool="antismash"
                     /translation="KETTLHAFEHQNYPFEELVDKVQVDRDLSRNPLFDTLFTM"
     aSDomain        complement(829..1683)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EC604_RS29285_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.60E-107"
                     /label="EC604_RS29285_Condensation_LCL.1"
                     /locus_tag="EC604_RS29285"
                     /protein_end="480"
                     /protein_start="195"
                     /score="350.9"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIISDG"
                     /tool="antismash"
                     /translation="EVYPQSFAQKRLFILNQLEGAELSYNMPEAMVLEGALDRARFEDA
                     FHKLVERHEMLRTGFEMVDGEASQRIYQELDFSVEFYSADEQEVEEIVRRFVRPFDLGK
                     PPLLRVGLVQLTPERHILMYDMHHIISDGVSMEIFVEEFVRLYGGEQLEPLRIQYKDYS
                     VWLHSQEQKERHQHQEAYWLNMFQGEIPVLEMPTDYPRPAVQSYEGHTLEFFLEASKTD
                     ALKQLASETGTTLFMVLLAAYNVLLHKYSGQEDMVVGTPIAGRNHGDVQPLIGIFLNTL
                     AIRS"
     CDS_motif       complement(832..921)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_EC604_RS29285_0004"
                     /evalue="9.30E-13"
                     /label="C5_LCL_267-296"
                     /locus_tag="EC604_RS29285"
                     /protein_end="479"
                     /protein_start="449"
                     /score="40.5"
                     /tool="antismash"
                     /translation="DMVVGTPIAGRNHGDVQPLIGIFLNTLAIR"
     CDS_motif       complement(1180..1212)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_EC604_RS29285_0003"
                     /evalue="2.10E-03"
                     /label="C4_LCL_164-176"
                     /locus_tag="EC604_RS29285"
                     /protein_end="363"
                     /protein_start="352"
                     /score="10.9"
                     /tool="antismash"
                     /translation="QYKDYSVWLHS"
     CDS_motif       complement(1243..1308)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_EC604_RS29285_0002"
                     /evalue="1.30E-10"
                     /label="C3_LCL_132-143"
                     /locus_tag="EC604_RS29285"
                     /protein_end="342"
                     /protein_start="320"
                     /score="33.7"
                     /tool="antismash"
                     /translation="MHHIISDGVSMEIFVEEFVRLY"
     CDS_motif       complement(1504..1614)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_EC604_RS29285_0001"
                     /evalue="1.30E-16"
                     /label="C2_LCL_024-062"
                     /locus_tag="EC604_RS29285"
                     /protein_end="255"
                     /protein_start="218"
                     /score="52.9"
                     /tool="antismash"
                     /translation="SYNMPEAMVLEGALDRARFEDAFHKLVERHEMLRTGF"
     aSDomain        complement(1729..1935)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EC604_RS29285_PCP.1"
                     /evalue="9.60E-24"
                     /label="EC604_RS29285_PCP.1"
                     /locus_tag="EC604_RS29285"
                     /protein_end="180"
                     /protein_start="111"
                     /score="75.4"
                     /tool="antismash"
                     /translation="ERKLASIWQEVLGLTKEIGVYENFFDIGGHSLRATTLVSKIHKEL
                     NVDLPLREVFRHSMIENMAVAISR"
ORIGIN
        1 caagcttcgg ctcgctctcg tagtggcgtt tgcgttaata gtaccttcgc tccgctgtct
       61 tcgagcatga accgcacgcg atctgccggg taatccggat cgagcggtac atacgcgccg
      121 cctgctttcc acacagccat cacggctaca agcaattcca ccgaacgttc ggcaaaaatg
      181 ccaacgatgg attcccggcc gatgccgctt gcgcgcaatg tagatgccaa acgattcgcc
      241 cgctcattca gctccgcata cgttaggcgg tcattctcgt acacaaccgc ctccgattca
      301 ggtgcgcgct gcacctgttc ttcaaacaga cggtggaacg cagccgcaag agtaacttcc
      361 acttctagtt gcgccgggtt aaatgagtcg agcatttctt tttcctcagt ccttaacaag
      421 ctcagctcag caatcttcgc atccggacgg cttacaatcg tgacgagcaa ctgctcgtaa
      481 tgtttggcca gccgttcgat cgtctccctt ttataaagag ccgtcgcata ttcgaagctg
      541 taatccaagc cgccgttctc ctcacccaca tccaagctaa tatcaaactt tgcggtatcc
      601 attgcgctcg gataagatgt caagcggagc ccttctaact cgaattcatc gttctccgta
      661 ttctgcatcg taaacaacgt gtcaaaaagg ggattacggc ttaaatcacg gtcgacttgc
      721 accttgtcca ccaattcttc gaacggatag ttttgatgct cgaaagcatg aagggtcgtt
      781 tctttaattt cgttcaggta tgacaggaat gtcttctccg aagacggata actgcgaatc
      841 gccagtgtat ttaagaaaat cccgattaac ggttgcacat ctccatgatt ccttccggca
      901 attggcgtac caacaaccat atcttcctga cctgaatatt tgtgcagaag cacgttatac
      961 gcagcaagta acaccataaa cagtgtcgtg cccgtctctg aagccaactg cttcagggca
     1021 tccgttttcg aagcttctaa gaaaaactcc agtgtgtgac cctcatagct ctgtacagct
     1081 ggacgcggat aatcggttgg catttccagc accggaattt ccccttggaa catgttcagc
     1141 cagtatgctt cctgatgctg atgccgttcc ttctgctcct gcgaatgtag ccaaacggaa
     1201 taatctttgt actgaatacg taaaggctcc agttgttctc caccatacaa gcggacgaat
     1261 tcttcaacaa agatttccat cgaaacaccg tcagaaataa tatgatgcat atcgtacatt
     1321 agaatatggc gttccggagt aagctggaca aggcccactc tcagcaacgg aggtttcccc
     1381 aagtcaaacg gacggacaaa acgacgaacg atctcttcga cctcttgctc atccgccgag
     1441 tagaactcca cggaaaaatc caattcctgg taaatccgtt gtgaagcttc gccatccacc
     1501 atctcgaacc cggtgcgcaa catttcatgc ctctccacaa gcttatggaa tgcgtcttcg
     1561 aaccttgcac gatccaaagc accctctagc accatggctt ctggcatgtt gtaactaagc
     1621 tccgcacctt ccagttgatt caagataaag aggcgttttt gagcaaaaga ttgtgggtac
     1681 acctcacgtt tatccgccag tggaatggca acgaatttct gctcatctag ccgggaaata
     1741 gcaaccgcca tgttctcgat catagaatga cgaaacacct ctcgcagtgg cagatccacg
     1801 ttcagctctt tatgaatctt gctgaccaat gtcgttgccc gcagggagtg accaccgatg
     1861 tcgaagaagt tctcgtacac gccgatctcc ttcgtaagtc ccagcacctc ctgccatatc
     1921 gaggccaatt ttctttctag ctcattgcgt ggtgcaacat actccgttcc gcttcttgcc
     1981 tccccttcag gcgctggcag cgccttccgg tctatctttc cgttcggcgt cagtggcaga
     2041 cgctccatct ccacgaagta cgacggaatc atgtatcctg gtaagtcctg tgccaacgcg
     2101 ccacgtagat tgttcactgc cagatccgtt cctgcatccg gtgtgtaata tgcacataac
     2161 actttctgcc cttccgcatc agttcgaacc agcgccacgg cttcgcgtac gcctttcacc
     2221 tgtagcaact tcgcttcgac ttcgcccgtc tcaatccggt agccccgaat t
//
