LOCUS       NZ_RIAS01000002        20246 bp    DNA     linear   CON 13-MAY-2024
DEFINITION  Paenibacillus amylolyticus strain 27C64
            NODE_2_length_688731_cov_20.1817_ID_3, whole genome shotgun
            sequence.
ACCESSION   NZ_RIAS01000002
VERSION     NZ_RIAS01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Paenibacillus amylolyticus
  ORGANISM  Paenibacillus amylolyticus
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 688731)
  AUTHORS   Keggi,C. and Doran-Peterson,J.
  TITLE     Paenibacillus amylolyticus 27C64 has a diverse set of
            carbohydrate-active enzymes and complete pectin deconstruction
            system
  JOURNAL   J Ind Microbiol Biotechnol 46 (1), 1-11 (2019)
   PUBMED   30377865
REFERENCE   2  (bases 1 to 688731)
  AUTHORS   Keggi,C. and Doran-Peterson,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2018) Microbiology, University of Georgia, 120
            Cedar St, Athens, GA 30602, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 23-MAR-2015
            Assembly Method                   :: SPAdes v. MARCH-2015
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 93.0x
            Sequencing Technology             :: Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003719335.1-RS_2024_05_13
            Annotation Date                   :: 05/13/2024 02:42:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,967
            CDSs (total)                      :: 5,826
            Genes (coding)                    :: 5,778
            CDSs (with protein)               :: 5,778
            Genes (RNA)                       :: 141
            rRNAs                             :: 5, 10, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 10, 9 (16S, 23S)
            tRNAs                             :: 113
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 8 of 48
            Pseudo Genes (incomplete)         :: 39 of 48
            Pseudo Genes (internal stop)      :: 7 of 48
            Pseudo Genes (multiple problems)  :: 6 of 48
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:46:40
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 334729
            Orig. end                         :: 354975
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            RIAS01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20246
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="proteusin"
                     /region_number="2"
                     /rules="(PoyD or TIGR03793)"
                     /tool="antismash"
     cand_cluster    1..20246
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PoyD or TIGR03793)"
                     /kind="single"
                     /product="proteusin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20246
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10246](-)"
                     /cutoff="20000"
                     /detection_rule="(PoyD or TIGR03793)"
                     /neighbourhood="10000"
                     /product="proteusin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10246)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PoyD or TIGR03793)"
                     /neighbourhood="10000"
                     /product="proteusin"
                     /protocluster_number="1"
     gene            complement(405..1379)
                     /locus_tag="EC604_RS05235"
                     /old_locus_tag="EC604_05210"
     CDS             complement(405..1379)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017690249.1"
                     /locus_tag="EC604_RS05235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EC604_05210"
                     /product="ring-cleaving dioxygenase"
                     /protein_id="WP_123063130.1"
                     /transl_table=11
                     /translation="MLIKGLHHVSALTAHADQNYRFYTDIMGLRLIKKTVNQDDVSVYH
                     LFYGDEKGNPGTELTFFEIPMAGRTREGVNSISATSLRVPSDAALAYWAQRFDEYEVPH
                     GEIVQRGGRATLSFTDFEGMRLILVSDEHNSGVAGGKPWDRSPVPAEYGIIGLGPIHLT
                     VRDASLTTPVLTELLGFRAKGSYPAFTPGQPDVLVFESGEGGSGSEVHLEERNDLEQER
                     PGRGSVHHVAFRVDNEEELRQWVDRVHQFHFPSSGFVDRFYFRSLYFREANGILFELAT
                     DGPGFDTDEDFATLGESLALPPFLEGRRAEIEANLKPLDTMIR"
     misc_feature    complement(1445..1459)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 20.95"
                     /tool="antismash"
     gene            1683..2459
                     /locus_tag="EC604_RS05240"
                     /old_locus_tag="EC604_05215"
     CDS             1683..2459
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017690248.1"
                     /locus_tag="EC604_RS05240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EC604_05215"
                     /product="TIGR02206 family membrane protein"
                     /protein_id="WP_123063131.1"
                     /transl_table=11
                     /translation="MAYPPWLDPYDAEPFAAFSTSHISSMIIIFALVILLFLYRHRLRF
                     MSERSSRFMRIGLAGFMIGCEIVLQIWYVYGGIWSLRTSLPLELCSLSLLLSALLLLTR
                     KKWLYSALLFSGIAGALMAIVTPNLGYAFAHFRFIQFFVAHAMIILALLYMTWVEQIRP
                     GWRSVAGSMIFVNIAALVVYGIDVLLDANYMFLRHKPGTPSVLDMLGPYPVYILGEEVL
                     ALVLFSLLYLVLFVIPEWLKRISGKKVKQTQMHSKS"
     gene            complement(2570..3364)
                     /locus_tag="EC604_RS05245"
                     /old_locus_tag="EC604_05220"
     CDS             complement(2570..3364)
                     /NRPS_PKS="Domain: PKS_KR (9-172). E-value: 8.7e-15. Score:
                     47.0. Matches aSDomain:
                     nrpspksdomains_EC604_RS05245_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013915458.1"
                     /locus_tag="EC604_RS05245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EC604_05220"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_123063132.1"
                     /sec_met_domain="adh_short (E-value: 2e-35, bitscore:
                     117.6, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 4.3e-33, bitscore:
                     110.4, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAEQRLEGKVAIVTGGGSGIGQASAIRFAEQGAKVYMLDRTPENA
                     EETKQIIETSGGEAYVIECDISKPEHVEKAINQAATEAGKLDIVFANAGINGAMAPIET
                     MKIEDWDQTMEINMRGTFATVKYAIPHLKERGGSIIITSSINGNRVFSGIGFSAYASSK
                     AGQTAFTKMAALELARYKIRVNAVCPGAIDTNIDDNTYPSDDLKDVQIPVEFPEGHEHP
                     LKGDPGTSEQVANLVLFLASDEASHVTGTRIYVDGAESLLRG"
     aSDomain        complement(2849..3337)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EC604_RS05245_PKS_KR.1"
                     /evalue="8.70E-15"
                     /label="EC604_RS05245_PKS_KR.1"
                     /locus_tag="EC604_RS05245"
                     /protein_end="172"
                     /protein_start="9"
                     /score="47.0"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VAIVTGGGSGIGQASAIRFAEQGAKVYMLDRTPENAEETKQIIET
                     SGGEAYVIECDISKPEHVEKAINQAATEAGKLDIVFANAGINGAMAPIETMKIEDWDQT
                     MEINMRGTFATVKYAIPHLKERGGSIIITSSINGNRVFSGIGFSAYASSKAGQTAFTKM
                     "
     CDS_motif       complement(3263..3331)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_EC604_RS05245_0001"
                     /evalue="2.20E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="EC604_RS05245"
                     /protein_end="34"
                     /protein_start="11"
                     /score="17.1"
                     /tool="antismash"
                     /translation="IVTGGGSGIGQASAIRFAEQGAK"
     misc_feature    complement(3451..3460)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     gene            complement(3519..3779)
                     /locus_tag="EC604_RS05250"
                     /old_locus_tag="EC604_05225"
     CDS             complement(3519..3779)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015736189.1"
                     /locus_tag="EC604_RS05250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EC604_05225"
                     /product="YqkE family protein"
                     /protein_id="WP_123063133.1"
                     /transl_table=11
                     /translation="MAKSKKHTPAPKAQNQDKPTTLKDLLSSDVLEKLKAQADEAKAAE
                     AERKEQERLQAEEARKAEQKRRDNDFEYLLNNSSMDWKKHK"
     misc_feature    3987..4000
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: medium, score: 20.49"
                     /tool="antismash"
     misc_feature    4135..4146
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.11"
                     /tool="antismash"
     gene            complement(4213..4875)
                     /locus_tag="EC604_RS05255"
                     /old_locus_tag="EC604_05230"
     CDS             complement(4213..4875)
                     /EC_number="5.2.1.8"
                     /GO_function="GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /GO_process="GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017690243.1"
                     /locus_tag="EC604_RS05255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EC604_05230"
                     /product="peptidylprolyl isomerase"
                     /protein_id="WP_123063134.1"
                     /transl_table=11
                     /translation="MSLRWKKTTAVSLVFAMLLIVMSGCVRPASNATQTPVPAAPEGPN
                     PVATIEMQDGQKIVIELYPEIAPNTVYNFISLANKGFYDGLIFHRVIPGFMIQGGDPDG
                     KGTGGPGYAIKGEFTSNGHKNHLKHTRGVISMARQSDNLDSAGSQFFIMLADADYLDNS
                     YATFGKVTEGMDVVDGIAAQQIGEGDKPVTDQVMKKVTVDTHGLEYPEPVKITEEKK"
     gene            5134..6345
                     /locus_tag="EC604_RS05260"
                     /old_locus_tag="EC604_05235"
     CDS             5134..6345
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017690242.1"
                     /locus_tag="EC604_RS05260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EC604_05235"
                     /product="MFS transporter"
                     /protein_id="WP_123063135.1"
                     /transl_table=11
                     /translation="MNFSWKRNLIILWIGVFFCSTAYSISIPFLPLFLSSDLGVHNHLE
                     LWSGLSFGITFLASALVSPFWGSLADKYGRKPMLIRSGYSLAVLYLINFFVQDPYSLIV
                     VRLFQGLLAGFVPAAIALVGTNTPEEKTGYALGIMSTAGATGGIIGPLIGGLVSHYYGN
                     RSAFLFSAIVVLVSAIIATFWVKEENFNRNKARSHVMDDIRKARANRVFVTVLGMMGIC
                     TFSVMILEPLLTVYVMEMGVQPDRASLSSGIIFSAVGVATVIMAPRWGKLGSRIGFGKV
                     LVIGLIGGAVGNLLQFFTTGYVAFGILRFVYGLFFAAVFPAINAMIVQVTEPAFRGRAF
                     SLNQSAAQIGTMAGPIIGGVLGGWLPIRWIFIINGLALLATAIVVKWSRLEQKLPGVTK
                     PVEK"
     gene            complement(6549..6746)
                     /locus_tag="EC604_RS05265"
                     /old_locus_tag="EC604_05240"
     CDS             complement(6549..6746)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006208682.1"
                     /locus_tag="EC604_RS05265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EC604_05240"
                     /product="hypothetical protein"
                     /protein_id="WP_123063136.1"
                     /transl_table=11
                     /translation="MTQNKKEEQVKKNNNLVTERDIDPDFGLFTEDSFPGALEDEDQRG
                     AIKHAVPEEQTSDEPSSDHK"
     gene            complement(6787..7164)
                     /locus_tag="EC604_RS05270"
                     /old_locus_tag="EC604_05245"
     CDS             complement(6787..7164)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EC604_RS05270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="EC604_05245"
                     /product="hypothetical protein"
                     /protein_id="WP_240036667.1"
                     /transl_table=11
                     /translation="MSDKPISNAESDRYYDRYQRSRDIPPETEVPEGDMDTFDEVSGRR
                     IEIEMEDSELPPVVSSAADEEGLESRLTETPLESVPDADLVQPGSPVDPAAPDPDALHG
                     TDLLNGAGADAKPQRNILPPR"
     gene            complement(7297..7458)
                     /locus_tag="EC604_RS30110"
     CDS             complement(7297..7458)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017690239.1"
                     /locus_tag="EC604_RS30110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_192895060.1"
                     /transl_table=11
                     /translation="MNEPHRTVEVEHKDVQHKSDSSVVASTFIKYAAYIIIFFGFLYFL
                     VKYVFPKF"
     gene            7604..7885
                     /locus_tag="EC604_RS05275"
                     /old_locus_tag="EC604_05250"
     CDS             7604..7885
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017690238.1"
                     /locus_tag="EC604_RS05275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EC604_05250"
                     /product="hypothetical protein"
                     /protein_id="WP_090917871.1"
                     /transl_table=11
                     /translation="MDWYTLGNMITRIRIGQKASTPGFSRTVIRRPDGLFWVGGIWSGQ
                     VVQLRDFLFSDIWTIYDDEETEQWLEFRTKVEQKEREMIENQFEDLRN"
     gene            8039..9940
                     /locus_tag="EC604_RS05280"
                     /old_locus_tag="EC604_05255"
     CDS             8039..9940
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017690237.1"
                     /locus_tag="EC604_RS05280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EC604_05255"
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="WP_123063137.1"
                     /transl_table=11
                     /translation="MRNVPKAIIILWMTIWTILCMPHGLVLAAPEEIQRPVSVTGWEVK
                     WGNAGDQGFISEVMGQDEIWEKRSAERIVDSSKEPSGSLWTRLTIPHMTDGSSAIRFEN
                     IKGTHIVIYLEDRKVYENYHYNYDNNAVFLPLSAENSGKTLYIWAQNERGWLGIQGDVQ
                     VGPYAMLQEKYLHNGLMDIILGSTFVFTSLIMLSCTFFLGQFHKGLWISLCVVMGSIGT
                     MVITYSQFLYTFYQVYGDLYSILFDLAMLLGMPALCYFFEQVIGPGVHGVFTKLRKVQW
                     AYSIVSVFSLFVYVISGGQWDLLYSLFVQHVVGIILVIVLTILLAGTIARALQRNREAI
                     LLAVGFGTFSLISVMELLWYYQRNGAYHLLWWKWSMVAFVISLIAILGSRFAEKHTKVL
                     EYSRELELFNNELQRSEKMEIISELAASVAHEVRNPLQVTRGFLQLMTEQEDNKNKGYV
                     RIALEELDRASGIITDFLTFAKPEFDHIISLNISDEFDHIEGILVPMANLEGGKITTDI
                     PPNIYIRGNSSKFKQAFINIIKNSIEAFQGGQGQIDIWAYSQDGKVKVHVRDNGEGMSE
                     EALSRLGEPYFSNKIKGTGLGMMVTFRIVEAMNGQISFTSTKGVGTEAVVSFPEFVK"
     gene            complement(10001..10246)
                     /locus_tag="EC604_RS05285"
                     /old_locus_tag="EC604_05260"
     CDS             complement(10001..10246)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     proteusin: TIGR03793"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017690236.1"
                     /locus_tag="EC604_RS05285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EC604_05260"
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="WP_123063138.1"
                     /sec_met_domain="TIGR03793 (E-value: 4.5e-20, bitscore:
                     66.4, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMVSEKTLHEDIIEKAWTDEHFRQQLHSNPKQALREAFGITIPDH
                     IKIRTVEEQQNDYVLVIPPNPSKVNYDVNCGPWRSF"
     gene            complement(10272..11249)
                     /locus_tag="EC604_RS05290"
                     /old_locus_tag="EC604_05265"
     CDS             complement(10272..11249)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EC604_RS05290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="EC604_05265"
                     /product="hypothetical protein"
                     /protein_id="WP_123063139.1"
                     /transl_table=11
                     /translation="MHRDWLDPYLLDVKAVFDSVEERLRQYPDSLAGHALEQLRLLNPL
                     RGDSGHSCIGYITPLWMQYTEGFPPDQARQLSAACLLHMMYFLNLDDVMDEPTEDAVLK
                     LSLGNLCYVDALQLYNALFESSSVFWNHFRQVLQDWAVSVNGELQADYFQRNPLLIAQK
                     ASPTQLGVIGSLLLLEQPDRIPSVCGALNIVLMTLQMTDDFTDTKMDAVHGNYNSYLSH
                     LSTALQQEYPTSHVLKESIHHNVYNSQLMNSYADIAQHFNDIIASSNSALPQFVVLNSY
                     LCNSLVQAIQEIEQRKKRLYRGGFHYWISEPQSGPSNTGQADSV"
     gene            11465..12199
                     /locus_tag="EC604_RS05295"
                     /old_locus_tag="EC604_05270"
     CDS             11465..12199
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EC604_RS05295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="EC604_05270"
                     /product="hypothetical protein"
                     /protein_id="WP_123063140.1"
                     /transl_table=11
                     /translation="MKINWKSWLATSAAVIVIAVAPSQIALADPVSPAEQQDKNLTESV
                     GGNKKPCMHAGHGLFMIGETSDLLGIGLRDLKQQMQLGKTLAQIAKERKGLSEEQLLQK
                     LKPTLSERLDLAVKEGCLTKEQAATAKANMDVKLKKVVNTPLTELRREFTHHGNHSMVD
                     KGAIAKFIGISPEQLQEQLHAGKSLVEIAQAKGISETQLVNQLKEQLTGDLQRFVHQKH
                     HGHSVPVHPGHVPGRSTSTEVK"
     gene            complement(12390..13634)
                     /locus_tag="EC604_RS05300"
                     /old_locus_tag="EC604_05275"
     CDS             complement(12390..13634)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1088: acyltransferase 3"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017690233.1"
                     /locus_tag="EC604_RS05300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EC604_05275"
                     /product="acyltransferase"
                     /protein_id="WP_123063141.1"
                     /transl_table=11
                     /translation="MKKPKIAEWTELRGLAFLAIVMQHNIAEYIYRPDIEQPDSVMLTM
                     IYHLTRFGTPTFVFLSGVLLFYHHRHIKPDYPRFIRKRFGDIYMPFFIWTFVYWLAVRV
                     FTPTFWTSGMPDLGSFVHELFIPQTGYHLWFVLMVFQFYILFPLFLAGAHAVRKRLDNL
                     KRFTSKQVTLTIILVAAILYLWLMKWSYYDMADWTQSWPDPWSSMLEYRSYSWLLYWFY
                     FLLGAVCAWTVESWRNWTLKALPCMATVFVAMYIWMGYDVLRGSENTMNLNISTYLKPT
                     TFISIMAQMLMLYGFLVLLNGKNLWFQRLLAWIGRYSFGGYLVHALVIYAIAYVTRPLH
                     LSGWHLPVAIISFVVTVVTALGISWTLSKLPGSRLSVGLTRSKARQVTSFSNSLAGSGR
                     RTRNQNPPLGSASKN"
     gene            complement(13770..14777)
                     /locus_tag="EC604_RS05305"
                     /old_locus_tag="EC604_05280"
     CDS             complement(13770..14777)
                     /GO_process="GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014278528.1"
                     /locus_tag="EC604_RS05305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EC604_05280"
                     /product="LCP family protein"
                     /protein_id="WP_123063409.1"
                     /transl_table=11
                     /translation="MFKKWLWGTALTLALVVTGVIVYYGYSIVHFANSISTAAPSDSPG
                     SNLDSNHTESSETANVVIPKWDGKERVNILLIGGDSRGDDAGRSDSVMVASIDPVSKKA
                     HLFSVLRDTYVDIPGHGKSRLNAAFSYGGAELTKQTVSSLLGIPIQHYVYTDFTGFMAL
                     VDALDGIDIDVEKDMYYTSKADKHMYDIDLKKGLQHMDGRTALQYVRFRHDATSDFTRT
                     QRQRIFMTELAKKMQSTTSLFKIPEMLEAIAPYIKTDLSPTQMLKLASLGFDIRVNEID
                     QQQIPPNKLLTNERAGSAQVLGVNVPKLQAYIQKLFEEDKQSSSAGSVTGEANE"
     misc_feature    complement(15042..15056)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 21.9"
                     /tool="antismash"
     gene            15146..16888
                     /locus_tag="EC604_RS05310"
                     /old_locus_tag="EC604_05285"
     CDS             15146..16888
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1061: methyl-accepting
                     chemotaxis protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683827.1"
                     /locus_tag="EC604_RS05310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EC604_05285"
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="WP_240036668.1"
                     /transl_table=11
                     /translation="MGKGKMRGNALGKVKFSIRSKLLTGFMIVLALLIFVSVYTLTQVF
                     TMADKSRQIDKTWMPTVTLLGIMNGDVSDVERLGLAIIVEQNPSETEKLNEALKVLLAK
                     IEDERKQLLTLIKGNAEAEKLYEQFSTSYDSYLQKMPEFIEYGLKNDYATSSTLHTAAY
                     PMWYTANDLITQLINLGNSGAKEISAGSVKLAENAFNIILGVAIVATLLSLLIALFIAS
                     MISRPVKKMSEAAAAIAEGDLTGEAITLKNRDELGVLAASFNTMSQNLRSMIQSVSETS
                     EQVAASSEELLASAEQNAKASEQITETVEELSVGTSNQVDIVKRSSQAMNEMALGSEQI
                     AELAQSVSVSAVDAANQSSEGNKIIQQAVDQMGSVRNSIASLRELVTGLGERSAEIGTI
                     TEVINNIARQTNLLALNAAIEAARAGEHGRGFAVVAGEVRKLAEESSASAQQITQIVQL
                     IQQDTDHAVQAVKVNSNETEAGIELVAAAGQAFEQISDAANKVAGEIQEVSAGSEEMSA
                     STNEVVSYVGQISNIASEAAGSVHNVSAATEEQLASMEEIASSAGLLSKMAEELQGQVN
                     KFKV"
     misc_feature    17018..17038
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 24.56"
                     /tool="antismash"
     gene            17225..20146
                     /locus_tag="EC604_RS05320"
                     /old_locus_tag="EC604_05290"
     CDS             17225..20146
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017690230.1"
                     /locus_tag="EC604_RS05320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EC604_05290"
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="WP_240036691.1"
                     /transl_table=11
                     /translation="MNTGLRQADAAAGDHNYAEALQKAIYFYEAQRSGPLPANNRVEWR
                     GDSGMQDGADVGVDLTGGWYDAGDHVKFGFPMAASATMLAWSVVEYSDGYEQAGQLEEI
                     KDNIRWATDYFMKAHTKPNELWGQVGGGNTDHAWWGPAEVMQMNRPSFKIDASCPGSDL
                     AAETAAALAAASIVFADDDPTYSAKMLQHAKELYNFADTYRGKYTDCITDAAAFYNSWT
                     GYEDELAWGGAWLYLATNDNAYLSKAMAAADRWSTSGGSANWPYTWTQGWDSKHYGAQI
                     LLARITSSLNMPEATKFIQSTERNLDYWTVGTNGGRVTYTPGGLAWLDQWGSLRYTANA
                     AFIAFVYSDWVSDPVKKSRYQNFATSQMNYILGDNPRQSSYVVGYGKNPPQHPHHRTAH
                     SSWMNNEDIPANHRHILYGAMVGGPNASDQYTDDISDYVSNEVATDYNAGFTGALAKMN
                     LLYGQNHQPLANFPAPEVKGEEYFVEAAVRSSGSNYTEIRALLNNRSGWPARMGDQLSF
                     KYFLDLSEVYAAGRTVSDVQVTVSSNEGATVSQPVVVDAAKRIYAITADFSNTKIYPGG
                     EGNYRKEVQFRITGPQGAWNPANDPSYQNLTTGTPVKSVYIPVYDAGVKVYGQEPGVTP
                     VTVPAAPSGVQALPGNNQVTLNWAASAGAVSYTVKRAEVSGGPYTTVATGVNGVTYTNT
                     GLTNGKTYYYVVTAVNAGGESPASVQVMGTPQAVSSVPGTLTLSGTAGNNQNVLSWTAA
                     SGAASYKVQRANANGTYADVATGLTVMTYTDTTALNGTSYNYRVVAVNASGQSLSNVVV
                     LTPSGPPVSTGTLEVQYRSGGSGNSSNAVTPQFNVKNTGTQPIDLSTVKIRYYFTKDGT
                     EDLTFWCDYAVVGTANVQGKFVTVNPAKGTADTYLEISFTSGAGSLAAGAETGVIQGRF
                     SKNNWSNFDQSNDYSYDGSKTAFAAWNKVTAYQGSTQVWGLEP"
ORIGIN
        1 atgatatcca gattaatacg ggtaaaaagt attcttctct gcacttggaa cttgccgcac
       61 taaagggaaa tcaggagatt catatctatg atcaggatct cgcgacactc aaaaagttca
      121 atatcactcc ggaaagcggt atgatgagtc tagatgtaga tgtgaaaggt acagatgctg
      181 tgtatgtcga gattgtatcc gaagatccag gttcagcctt gtttgtacca cttactacat
      241 cgtatctgac caagtaaaac agactatgta tgctggttaa taaaaaacga tgattcacca
      301 ggcatcctct gactcaagtc ttgaatacag agctgtgtgg tgatcatcgt tttattttca
      361 ttttattgaa cattcaatgt tgacttaccg gacaatcacg aatattagcg gatcatggta
      421 tccagcggtt tcaggtttgc ttcaatctca gcacggcgtc cttccaggaa cggaggtaac
      481 gccagtgatt ctccaagtgt ggcgaagtcc tcgtccgtat cgaatcccgg gccatctgtc
      541 gcaagctcaa acagaatacc gttagcttca cggaagtaca gggaacggaa gtagaagcgg
      601 tctacaaaac cggagcttgg gaagtggaac tgatgtactc ggtcaaccca ctgtcttaac
      661 tcttcctcat tgtctacacg gaatgccaca tggtgcacac tgccgcgacc tggccgctct
      721 tgttccaaat cgttgcgttc ttccaaatga acttctgaac cgctgccgcc ttcaccagat
      781 tcgaacacaa gtacatcagg ttgacctgga gtaaacgcag ggtagctgcc ttttgctcta
      841 aatccaagca actcggtgag aacaggtgta gtgagtgaag catctcgtac agtcagatga
      901 attgggccga gtccaatgat gccatactct gctggcacag ggcttcgatc ccaaggttta
      961 cccccggcaa cacccgagtt atgttcatcg gaaacgagaa tcagccgcat accttcaaag
     1021 tctgtgaatg acagtgttgc acggcctcca cgttgcacaa tctcgccgtg aggtacttca
     1081 tattcgtcga agcgttgtgc ccaatacgca agagctgcat cacttggcac acgaagtgag
     1141 gtagcagaga tgctgtttac accttcacgt gtacgcccag ccatcggaat ttcgaagaag
     1201 gtaagctctg tgccgggatt tcctttctca tctccataaa acaggtgata gaccgaaacg
     1261 tcatcttgat tgaccgtttt tttgatcaga cgaaggccca tgatgtccgt atagaaacgg
     1321 tagttttgat ctgcatgtgc ggtaagcgcg gatacgtggt gaagcccttt gattagcata
     1381 atatattcct cctagagata gagttgaagt cttatgaaag tttgttgaat gtactagaga
     1441 gcttaaggtt ctgaaaaata agtcagaatg aatgaagagt ggaaaacaga tatttaattt
     1501 aaagttacta actttttgta tgttttattc ttggttttat cttaacataa agatatttag
     1561 aatacaatac ttatttgaat gaagctggac aggggtagag atcagtggca aaatgaaaaa
     1621 gttaccattc acgttataag aagtcacctc tgttaaccag aataaaagga gagaagaaag
     1681 gcatggctta cccaccttgg cttgacccgt atgatgcaga gccttttgcg gcattctcga
     1741 cttcacatat tagttcgatg atcattattt ttgcacttgt tatcctgtta tttctttaca
     1801 ggcatcggct tcgtttcatg tctgaacgct caagccggtt catgcgcatc ggtctggcag
     1861 ggtttatgat cggttgtgaa attgtactgc aaatctggta tgtgtacgga gggatttgga
     1921 gtctgcggac ttcactaccg ctagaactgt gcagcctgtc gttattatta tcagcgttgt
     1981 tattactaac acgcaaaaaa tggttatatt ctgccttact tttttcaggt atcgcaggtg
     2041 ctctgatggc catcgttacg ccgaatctcg gttatgcttt tgctcatttc cgcttcattc
     2101 aattctttgt cgcacatgcg atgatcattc ttgctctgct ctatatgact tgggtggaac
     2161 aaattcgtcc tggatggcga tctgttgctg gctccatgat atttgtaaat attgcggcgc
     2221 tcgtggtata cggtattgat gtattgctcg atgcgaatta tatgtttttg agacataaac
     2281 cgggcacacc gtctgtctta gatatgcttg ggccttatcc ggtctacatt cttggagaag
     2341 aggttctggc tctggtgtta ttctccttgt tatacctggt gctctttgtg attccggagt
     2401 ggctgaaacg aatcagtgga aaaaaggtaa agcaaacgca gatgcattcc aaaagctaat
     2461 tttcctgaag tgaattttaa ttaagataac ccgcaaacct tctggctatc cagagcattt
     2521 gcgggtttgt tatatttctg tcatcggagt ggggtgtaaa ttaataactt catccgcgca
     2581 gaagggattc ggcgccgtcc acgtagattc gtgtgcctgt tacatgagag gcttcatctg
     2641 aagcgaggaa cagtaccaga ttggcgactt gctccgatgt tccaggatca cccttgagtg
     2701 gatgctcatg tccctcagga aactctactg gaatctgaac atctttcaga tcatctgatg
     2761 gatacgtatt gtcgtcaatg ttggtatcga ttgcccctgg acaaacggcg ttgacacgaa
     2821 tcttgtagcg agccagctcc agtgcagcca ttttggtgaa agccgtttga cccgctttgc
     2881 tggaagcgta tgcagaaaat ccgatgccgg agaatacccg gttgccgtta atggaactcg
     2941 taataataat actgccgcca cgctctttca gatgagggat cgcatacttc acggttgcaa
     3001 acgtgccacg catgttaatc tccatcgtct gatcccaatc ttcgattttc atcgtttcaa
     3061 ttggagccat ggcgccatta atccctgcat tagcaaaaac gatatccagc ttgcctgctt
     3121 cggttgccgc ctggtttatg gctttctcta catgttccgg cttggagata tcacattcga
     3181 tcacgtaagc ttcgccaccg gatgtttcaa tgatttgttt cgtttcctct gcgttctcgg
     3241 gtgtacgatc taacatgtat actttggcac cttgctctgc gaatcgaatt gcagaggcct
     3301 gaccaatacc tgagccgcct ccagtaacaa ttgctacttt gccttccaac cgttgttcag
     3361 ccattcattg atccctccat gtatgaatta tccttgaaca ctctatttct aaatacccga
     3421 catgagggag gtgaatcata tgtgtacata taaaaaaaca gagcccaatt cggaccctgt
     3481 ctccagagta gcgttatatt ctctttttgc tcccttggtt acttatgttt cttccagtcc
     3541 atggagctgt tattcagcag gtattcaaag tcattgtccc ggcgcttctg ctccgccttg
     3601 cgagcttcct ctgcctgtaa acgctcttgt tctttacgct ctgcttcagc agctttggct
     3661 tcgtcagctt gtgcttttag cttctcgagc acatcactgc tcagcagatc tttcagcgtg
     3721 gttggtttgt cctgattctg cgccttgggc gcaggagtat gttttttgga tttagccact
     3781 tggaaaatct cccttcaaaa catgaatcat catccatatc gttattgtag tcatagtata
     3841 tcaggttcgt gttgtgcatt ccatgaagtt acagagcagg cgcaggaaaa agggcatgtc
     3901 acggcgaatg aagacaggta taaaaccata taatggtacc gatactacga ctattccctg
     3961 aagaaaggag tcgttgttca tggaggcaaa ccgtagtttg ttgaaagggc ttgcatgggg
     4021 gatggccttt agtttcccac tatggattgc ggttatcggg tggctggatt tgttgggatg
     4081 gatgcattga agtgctactc acatccaaaa aagaggtttg atctagttat cgatgtaaac
     4141 gttatcatta aataagctct gctctgtgac acatgcacag aacagagctt ttgtttgttc
     4201 atagaagaga ggttacttct tctcctccgt aatcttcaca ggttctggat attccagtcc
     4261 gtgtgtatca acagttactt ttttcatgac ttggtccgta accggcttgt ctccttcgcc
     4321 aatctgctga gctgcaattc catcaactac atccattcct tcggtgactt taccaaaagt
     4381 agcataagag ttatccaaat aatcagcatc agccagcata atgaagaatt gagatcctgc
     4441 agaatcgaga ttgtcagatt ggcgtgccat cgaaatcact ccgcgcgtat gcttcagatg
     4501 atttttatga ccgttagagg taaattcacc tttgatcgca tagcctggtc cacctgtacc
     4561 tttgccatcc ggatcaccgc cttgaatcat aaagcctgga ataacccgat ggaagatcag
     4621 gccatcataa aagcctttgt tcgccagtga gataaaatta tacaccgtat tgggggcaat
     4681 ctccggatac agctcaatga cgatcttctg tccgtcctgc atctcaatcg tagctacagg
     4741 attagggccc tctggtgctg ctggaaccgg agtttgtgta gcattgcttg ccgggcgtac
     4801 gcatccgctc ataacgatca gcagcatcgc aaagacaagc gagactgcgg ttgttttttt
     4861 ccatcgtaaa gacatcaata tgttcctcct tggttcgttg ttataacgaa atctcatcca
     4921 atatagtttg gttaattctt caactatcat accgtttcta ggctctgaat ggcaatgcaa
     4981 gccgtgttaa ttcggtgaac tggggatggt catcatgaga gcatgggtgt aaaataagga
     5041 aaatgcttcc gcatcagggg gacgtacggg tctgttccgt ccgctccaaa cacggcagtc
     5101 ccggcgtcag ttacggaaag gacgggcggg tatatgaact tctcgtggaa gcgtaatctg
     5161 atcattttat ggatcggtgt ctttttttgc agtacggcgt attcaatttc aattcctttt
     5221 cttcccttat ttttaagcag tgatctgggc gttcacaatc atctggaact ctggtcaggc
     5281 ctttcgtttg gcattacctt tcttgcaagc gctcttgtat cacccttctg gggatcattg
     5341 gccgataaat atgggcgtaa gccgatgtta attcgatcag ggtacagctt ggctgtcctg
     5401 tatctgatta atttttttgt acaagatccg tattcactga ttgtggttcg tttgtttcaa
     5461 gggttgcttg ccggatttgt tcctgcagcg attgcccttg taggcacgaa cacacctgag
     5521 gagaagaccg gatatgcttt gggaattatg tcgaccgcgg gggctacagg tggtatcatc
     5581 ggtccactga ttggtgggct tgtcagccat tattatggca accggagtgc gttcctattt
     5641 tcggcaatag tcgtgctggt gtctgcgatc attgcaacct tctgggttaa ggaagagaat
     5701 tttaatcgta acaaggctcg ttcccatgtt atggatgata tccgtaaagc gagagcgaat
     5761 cgtgtgtttg tcaccgtgct tggcatgatg ggcatctgta ccttttctgt tatgattctc
     5821 gagccgctat taaccgtgta tgtcatggag atgggcgttc agcctgatcg ggcttcctta
     5881 agctcgggga ttattttctc ggcggtagga gtggcgacgg tcattatggc tccccgctgg
     5941 ggcaagcttg gatcaaggat cggttttgga aaagtgttag tgattggact aattggcgga
     6001 gcagtgggta atctgctgca gttttttaca acaggttatg ttgcatttgg catcctgaga
     6061 tttgtctacg gactcttttt tgcggctgtg tttcctgcga tcaatgcgat gattgtgcag
     6121 gtgactgaac cggcatttcg gggacgggct tttagtctga accagtctgc agcccagatc
     6181 ggaacgatgg cagggccgat tatcggcggt gtccttggcg gctggctgcc gattcgctgg
     6241 atatttatca ttaacggtct tgcgcttctg gctactgcta tagtggtgaa gtggtccagg
     6301 ttggagcaga agcttccagg agtaactaag ccagtagaga agtaatcgtt ataatatagg
     6361 aagaagatga tgctggaaat gcagttgtca tcttttttct atagaaaccg gagccagctt
     6421 tgaagtgaaa ataaaagaaa ccccttgtta aggggttctc cagcatcact tttctcatat
     6481 tcagcgcaga gtctatacat acatatttgt acaatagatc acaacgttta tacttataat
     6541 tatttacttc atttatgatc cgaagagggt tcatcagaag tctgctcttc gggaacggca
     6601 tgcttaatcg caccgcgctg atcttcatct tccagtgcac cagggaaaga atcttctgta
     6661 aagaggccaa aatcaggatc gatatcccgc tcagtaacaa ggttgttgtt tttcttgacc
     6721 tgttcttcct ttttgttttg ggtcatgtca gcacctccta gtgtgagaat atgcaaatca
     6781 atacgtttaa cgtggcggca ggatattccg ttgtggtttg gcgtcagctc cagccccgtt
     6841 tagcagatcc gtgccatgca gggcgtcggg gtcgggtgcg gcagggtcaa ccggactgcc
     6901 cggttgcacc agatctgcat cgggtacaga ttccagtgga gtttccgtca agcgggattc
     6961 caaaccttct tcgtctgcag ctgatgaaac aacgggtgga agttccgaat cttccatttc
     7021 aatttcaatg cggcgtccag aaacttcatc aaaggtatcc atgtctcctt cgggtacttc
     7081 ggtttcaggc ggaatatcac gggacctctg gtaacggtca taatagcggt cactttccgc
     7141 gttgctaatt ggtttatcag acatcatcag cacttccttt catacgggtt aatagtacaa
     7201 cgaattctac attctaaaac catcatttca tcgtgtaatc atcataccta gcatgatatt
     7261 gtccccataa atagaccagg ttatgtcagg agttcgttaa aatttcggga atacatattt
     7321 cacaaggaaa tagagaaatc caaagaagat aatgatatat gcggcatatt taataaatgt
     7381 agaggcgacc acacttgaat ccgatttgtg ctgcacatcc ttatgttcaa cttctactgt
     7441 gcgatgaggc tcattcatta taatcatctc cttgtttagt gagttttgct ttgaaaaacc
     7501 attacacatg atccactgga ttgaatcgtc ccagccgttt ttacagcatt tcttgttttg
     7561 cctatgttat gatgaagcat gactactcaa ggaggggtgc agcgtggact ggtacacatt
     7621 aggcaatatg atcacacgta ttcggatcgg acagaaagcg tccacacccg ggttttctcg
     7681 tactgtcatt cgtcgtccgg atggattgtt ctgggttggc ggtatatggt caggtcaagt
     7741 tgttcaatta cgtgattttt tgttctctga tatatggact atttacgatg atgaagaaac
     7801 ggagcagtgg ctggagtttc gaacgaaggt ggaacagaag gaacgtgaaa tgattgaaaa
     7861 tcagtttgag gacttacgaa attaaagaca tatataacaa taaaatggaa aaaagactat
     7921 aaatctactt atatgtgaat tttgaaatta tgtaagaagg tgacatgata aacggtggcg
     7981 aattgatagg gtgagggaga aatgaagaac cgaaaataaa aactttgagg tgctgtgaat
     8041 gagaaatgtg cccaaagcta ttatcatctt gtggatgacg atatggacga ttctgtgtat
     8101 gccgcatgga cttgtacttg ccgcaccaga agagattcag cggcctgtat ccgttacagg
     8161 atgggaagtg aaatggggga acgctggaga tcagggcttc attagtgagg ttatgggcca
     8221 ggacgagata tgggagaaac gaagtgctga gcgaattgta gacagtagca aagaaccatc
     8281 cggttctttg tggacacgtt tgacaattcc ccatatgact gacggaagtt cagcgattcg
     8341 tttcgaaaat attaaaggta ctcatattgt catctatctt gaagatcgga aagtatatga
     8401 gaattaccat tataattacg acaataatgc tgttttttta ccgctatctg cagaaaattc
     8461 ggggaaaaca ctctatattt gggcgcaaaa tgagagagga tggttgggca ttcaaggtga
     8521 tgtccaggtc ggaccatatg ccatgctgca ggagaagtat ctccataatg ggctaatgga
     8581 tattattttg ggttcgactt ttgtatttac ctcgctgatt atgctgagtt gcacattttt
     8641 tctgggtcag tttcataaag gactatggat atcgctatgt gttgtgatgg gttccatcgg
     8701 gacaatggtg atcacttact ctcaattctt gtacaccttc tatcaagtat atggtgacct
     8761 gtattctatt ctgtttgatc tggccatgtt gctggggatg cctgcacttt gttatttctt
     8821 tgaacaagtt attggtcccg gtgtacacgg ggtattcacc aaattgagaa aggttcaatg
     8881 ggcctattcc atcgtttctg tattttcatt gtttgtctac gtgatatcgg gtggacaatg
     8941 ggatctattg tacagtttgt tcgtacagca tgtcgtgggc attatactgg ttattgtact
     9001 gaccatcctg ctggctggca cgatagcccg ggcactgcaa cgtaaccgtg aagcgatcct
     9061 gttggcggtt ggtttcggta cattttcact aatcagtgtc atggaacttc tttggtacta
     9121 tcagcgcaat ggggcgtatc acctgctctg gtggaaatgg tccatggttg cgtttgtcat
     9181 ctcgttgatt gctattcttg gcagtcggtt tgcggaaaaa cataccaaag tgttagagta
     9241 ctccagggaa ctggagctat tcaacaacga gttacagcga tccgaaaaga tggaaatcat
     9301 tagcgagctt gccgcatcgg ttgctcacga ggtgcgtaac ccgcttcagg tgacgcgagg
     9361 attcctgcaa cttatgacag agcaggagga caacaaaaat aaaggatatg tgcgcattgc
     9421 actggaagaa ctcgaccggg cttccggcat tattacggat tttctgactt ttgccaaacc
     9481 ggaattcgat cacattattt cactgaatat atcagatgaa tttgatcaca tcgaaggtat
     9541 acttgtacca atggcgaatc tggaaggcgg gaaaattaca accgatatcc cgccgaatat
     9601 ttatattcgt ggaaattctt ctaagttcaa acaggccttt atcaacatta ttaaaaatag
     9661 tattgaggca tttcaaggag gacaaggtca gattgatatc tgggcgtact cacaggatgg
     9721 taaagttaag gtgcatgtca gagataatgg tgaagggatg agtgaggaag ctttgtcccg
     9781 attgggagaa ccgtatttct ccaataaaat caaaggcact gggctaggta tgatggttac
     9841 cttccgcatc gtggaagcga tgaatggaca aatcagtttt acaagcacaa aaggggtagg
     9901 aacagaagct gttgtatctt ttcccgagtt cgttaaatga cggacttggg gaggataccc
     9961 agcataatgt tctacccctg atttttttat ttagccaaga ttaaaacgat ctccacggtc
    10021 cgcaattgac gtcataattg actttggaag ggttaggtgg aatgaccaga acataatcat
    10081 tttgctgctc ctcgactgtg cgaatcttga tatggtcagg aatcgtaata ccaaacgcct
    10141 cgcgaagtgc ctgcttagga ttggaatgga gttgttgtct gaagtgctca tctgtccaag
    10201 ctttctcaat aatatcctca tgcaacgttt tttctgacac catcatcgtc aacattccct
    10261 tcgtatttaa attaaactga atcggcttgt cccgtattag aaggtccgga ttgtggttcg
    10321 ctgatccagt agtgaaaccc acctcggtaa agtctttttt tacgctgttc tatttcctga
    10381 atagcttgta ccaatgagtt gcacagataa gaattcagga cgacgaattg tggcaacgcc
    10441 gagttagaag atgcaatgat gtcgttgaag tgttgagcta tgtctgcgta actgttcatc
    10501 aattgtgagt tatatacatt gtggtgtata gattccttta gcacatgact cgtcggatat
    10561 tcctgttgga gagctgtgga taggtgagag aggtagctat tgtagttgcc gtgtacagca
    10621 tccattttcg tgtctgtaaa atcatctgtc atctgtagag tcatgagaac gatattcagt
    10681 gcaccgcaga ccgaagggat tcgatcaggt tgctctagta aaagcaagga accaataact
    10741 cccaattgtg tcggtgaagc tttctgggcg atgagaagtg ggttacgctg gaagtaatct
    10801 gcttgtaatt caccatttac gcttactgcc cagtcctgaa gaacctgtct gaaatggttc
    10861 cagaagacag aagaggattc aaataatgcg ttatagagtt gaagggcatc cacatagcag
    10921 agattcccta aagataactt cagcaccgca tcctcagttg gctcatccat gacatcatcc
    10981 agattaagaa aatacatcat gtgtaacagg caagctgcgc taagctgacg agcttgatcc
    11041 ggcgggaatc cctctgtgta ctgcatccat agcggagtga tgtaaccgat gcaactgtgt
    11101 cccgaatctc ccctgagcgg gttgagcaaa cggagttgtt caagcgcatg ccctgccagt
    11161 gagtcagggt attgccgaag acgctcttca acggaatcaa acacggcctt cacgtcaagc
    11221 aaatacggat ctagccaatc tcgatgcatg atatgaaaac gtccttcctg cattaaaagt
    11281 tggaatatgc agtaagatca agaagttaca tacagtatat ctcaagtgtt ctgaaaaaaa
    11341 taggtttttt tgtgatcatt tgtgagaaaa atgatgacat tttggatttt atagcataaa
    11401 aagttgtaat ttgggttcag actaagaagg aagagaatat cgagacagga aaggtggata
    11461 aacaatgaaa ataaattgga aatcttggct ggcaaccagc gcagctgtta tcgttatcgc
    11521 tgttgcacca agccaaatcg ctttagctga tcccgtttct cctgctgagc agcaagataa
    11581 aaacctgact gaatctgtcg gaggaaacaa aaagccttgt atgcatgcag gacatggtct
    11641 gtttatgatc ggtgaaacat ccgatttgct cggaattgga ctccgtgatt tgaagcagca
    11701 gatgcagctt ggcaagacct tggctcagat tgcgaaggaa cgcaaaggac tcagcgagga
    11761 acagttactg caaaaactaa agcccacgct gtcagagcgc ttggatctgg ctgtgaaaga
    11821 aggctgtcta accaaggaac aagctgcaac agcgaaagcc aacatggatg tgaagttaaa
    11881 aaaggtagtg aataccccgc ttaccgaatt acggcgtgag tttacgcacc atgggaatca
    11941 ttccatggta gataaaggag cgatagcgaa atttatcggg atttcacctg aacaattgca
    12001 agaacaactg catgcgggca agtctttggt tgaaattgcg caagcgaagg gcataagtga
    12061 aacgcagttg gtaaatcagt taaaagaaca attaacaggt gatctgcaaa ggtttgttca
    12121 tcaaaagcac catggtcact ccgttccagt tcatccagga cacgtgcccg gtcgatccac
    12181 ctcaaccgaa gtgaaatgat ttacttcata gaacaagatg actagtgtac tgagcataat
    12241 gagcaagaat agagcgtcct ccgcaaggga gaacgctcta ttcttgcttg cattacactt
    12301 ctgagggtct aaggcccttg agtttcaata tcacatcgta aaacgagttc aacgattgtt
    12361 gccatgttaa ttggtccgcc ttttaacttt taattcttgg aagctgatcc aaggggcgga
    12421 ttttgattcc gggtgcgtct tccagaaccc gctaatgaat tgctaaaaga agtaacttgc
    12481 cgagccttgc tgcgtgttaa tccaaccgag aggcgggagc ctggcagttt ggataatgtc
    12541 cagctaatgc caagagctgt cacaacggtg accacgaatg agattatcgc tacgggcaga
    12601 tgccacccgc taaggtgtag cggtctggtg acatatgcaa tagcataaat caccaaggca
    12661 tgaacgagat aaccaccgaa ggaatagcgc ccaatccagg cgagcagacg ctgaaaccat
    12721 aggtttttac cattcagcag aacaaggaaa ccgtagagca taagcatctg agccataata
    12781 ctgatgaacg tagtgggttt tagatacgta gagatattga ggttcatcgt attttccgac
    12841 ccgcgcagta catcgtaacc catccagata tacatcgcga caaagaccgt agccatgcaa
    12901 gggagcgcct tcaacgtcca atttctccag ctttccaccg tccaggcaca taccgcgcca
    12961 agtaaaaagt agaaccagta caacaaccat gaatatgaac gatattccag catgctagac
    13021 caaggatctg gccacgattg tgtccagtca gccatatcat aataggacca tttcataagc
    13081 cagagataga gtatggctgc aacgagtatg attgttaacg tcacctgttt cgaggtaaaa
    13141 cgcttgaggt tatcgagccg tttacgaaca gcgtgagccc ccgcgagaaa taaaggaaac
    13201 agaatataaa actggaacac catcagcaca aaccaaaggt gataacccgt ctgcggtata
    13261 aataactcat gtacaaagct ccccagatcc ggcataccgg aagtccagaa cgttggggta
    13321 aatacacgta cagctaacca gtagacgaac gtccatatga agaaaggcat ataaatatca
    13381 ccaaatcgct tcctgatgaa gcggggataa tcaggcttga tatggcggtg atgatagaat
    13441 aacaacacac ccgacagaaa tacaaaggtc ggtgtaccaa accttgtcag atgataaatc
    13501 atggtcagca tcaccgaatc aggctgctca atgtccggcc gatagatgta ttcagcaatg
    13561 ttatgctgca tgacgatggc caagaaagct aatccgcgaa gttcggtcca ttcggctatt
    13621 ttcggctttt tcatgtacat tcctccctta tgtagccagg gtcatcatat aagactaccc
    13681 tccaaacatt aagggtgcat taaatggtga aaaaaggaac cgaaaaaaga cggcttaagc
    13741 cgtcttttta ctaattgatt atgtgatagt cactcgttag cttccccagt tactgagcca
    13801 gcggaagaag actgcttatc ttcctcaaat aacttttgaa tgtaagcttg aagcttgggc
    13861 acattcacac ctaatacctg agcagaccca gcccgctcat tggtaagcag cttgttcggc
    13921 ggaatctgct gctgatcaat ctcgttcaca cggatatcaa agccaagtga agcgagcttc
    13981 aacatctggg ttgggctcaa atccgtcttg atataaggag caatggcttc cagcatctcc
    14041 gggattttaa agagcgaagt ggtgctctgc atcttcttgg caagctcggt cataaagatg
    14101 cgctgccgct gtgtgcgcgt gaagtcggag gtcgcatcat gccggaaacg aacatactgg
    14161 agggcggtcc tgccatccat atgttgcagc ccttttttca aatcaatatc atacatgtgt
    14221 ttatccgctt tgctcgtgta gtacatatct ttttccacat caatatcaat gccatccagc
    14281 gcatcaacga gagccatgaa gcctgtaaag tccgtgtaga cataatgctg aatcggtata
    14341 ccgagcagac tgctgaccgt ctgtttggtt aattcggctc ctccatagga aaaggcagcg
    14401 ttaagacggc ttttgccatg gcctgggatg tcaacgtagg tgtcgcgaag tacggagaac
    14461 aggtgggctt tcttcgaaac aggatcaatc gaagcgacca tcaccgagtc tgaacgtcct
    14521 gcgtcgtccc ctctcgagtc accgccaatc aataagatat tgactctctc cttgccgtcc
    14581 catttcggaa tgactacgtt ggctgtttcg gagctttccg tgtggtttga atcaaggttc
    14641 gaaccgggtg aatcgctggg tgcggcggtc gaaatgctat tagcaaaatg aacgattgaa
    14701 tatccgtagt agacaatgac tcctgttaca acaagtgcca aggtcagggc tgtaccccat
    14761 aaccattttt taaacataat ctcacctttc ttatgtagaa ctgatagatc ccccaaacta
    14821 gtttaacaaa aagaaagtaa aatgtcctct ttgattccga atatacgttt taaatcaata
    14881 ttgttttacg aatagtttgt tagttgtggt tgggacactc attaaggagt ttcgtcgctt
    14941 tcttggaaaa ttcgcggcct ttcacaatgg tagatatctg tttctgagag ttatatataa
    15001 aattatgttc tgtcagatat atgaggctca ttgttaagag aaattttctt aaaactaaga
    15061 tttcatatat tcactcgaag aatatctatg tcgatatatt atatagatgt actagtttat
    15121 tggaggaagt tggaggagtg ggaacatggg aaaaggaaaa atgcggggga acgcattagg
    15181 gaaagtaaaa ttttcgattc gtagtaaatt attaactggc ttcatgattg tgttggcttt
    15241 gcttatcttt gttagcgtgt acacactaac acaagtgttt acaatggctg acaaatcaag
    15301 acaaattgat aagacttgga tgccaactgt aaccttgctt ggaattatga acggtgatgt
    15361 gtcggatgtg gagagattgg gactagccat cattgtagaa cagaatccaa gtgaaacgga
    15421 gaaactgaat gaagctttaa aagtattgct tgctaaaatt gaagacgagc gcaaacaatt
    15481 gcttacttta atcaaaggaa atgcagaagc tgagaaactg tatgaacagt tttcaacgag
    15541 ttatgattcc tatttgcaaa aaatgccaga attcattgag tatggtctga aaaatgatta
    15601 tgctacatcc agcacgctac atacagcagc ctatccaatg tggtatacag ccaacgatct
    15661 gattactcag ttaattaatc ttggcaatag cggagcaaaa gaaatttctg ccggatctgt
    15721 caagcttgcc gagaatgcgt ttaacattat tttaggtgta gcaatcgttg caacattgtt
    15781 atccttactc attgcgctat tcatcgcaag tatgatctca cgtccggtta aaaagatgag
    15841 tgaagccgca gcagcgattg ctgaagggga cctgacgggt gaggcgatta cattgaagaa
    15901 ccgggatgag ctgggtgttc tggcagcttc cttcaataca atgagtcaaa atctgcgttc
    15961 gatgattcaa tccgtatccg aaacatccga gcaagtcgcg gcttcatccg aagaacttct
    16021 tgcgagtgca gagcaaaacg caaaagcttc ggagcaaatt accgaaacgg tggaagaatt
    16081 gtctgtagga acatcaaatc aagttgatat cgtgaagcgt tcttcgcagg cgatgaatga
    16141 gatggcatta ggctccgaac agattgccga actggctcaa agtgtatccg tatctgctgt
    16201 ggacgcagcg aatcagtcgt cggaagggaa caaaattatc cagcaagctg tggatcagat
    16261 gggatcggtt cgtaactcca tcgcttcatt gagagaattg gtcaccggac tgggcgaacg
    16321 ttctgccgag attggtacga tcaccgaagt gatcaataac atcgcccggc aaaccaattt
    16381 gcttgctttg aatgcagcga ttgaagccgc aagagcaggg gagcatggca gaggttttgc
    16441 tgtcgttgcg ggagaggtgc gcaagctggc ggaagaatcc tccgcttctg cacagcagat
    16501 cacacagatt gtacaactga ttcagcagga tacggatcat gctgtacagg ctgtgaaagt
    16561 aaatagtaat gaaacagaag ccggcattga attggttgct gcagctggtc aagccttcga
    16621 acaaatttcg gacgctgcca acaaagtagc tggtgaaatt caagaagtat ctgccggttc
    16681 tgaggagatg tcagctagta caaatgaggt tgtaagttac gtgggtcaaa tctccaatat
    16741 tgcttcggaa gctgcgggtt ctgtacacaa cgtatctgct gcaaccgaag agcaactggc
    16801 ttccatggaa gagattgctt cctccgctgg gttgttatcc aaaatggcgg aggaattgca
    16861 aggacaagtg aacaaattta aagtatagat tgcaaaagca gatcgccaaa aagctgtttc
    16921 tcccggacat atccggtgaa gaaacagctt ttttgtcata tcacgctgct caagtcacaa
    16981 caatgttatt tcagaagtca ccaatgttga tatttcaaga caaacaaaag gctataatgg
    17041 taattaatgg tggaaattgc attgagttac caccatagag aggagcgagg tcacccagaa
    17101 tatgtgcaag taatcacctg gaactttcga gaaaaggaga tgaataggat ttatgaaagg
    17161 gcgctggtgg agacgcgttg cgatggtaac gttatcggca ggacttctcg cagggagctt
    17221 ttctatgaat acaggattac gtcaggcgga tgcggccgcc ggagatcata actatgccga
    17281 agcactacag aaggccattt acttctatga agcgcaacgc tcaggcccgt tgcctgccaa
    17341 caaccgggtt gaatggcgag gtgactcggg aatgcaggat ggggccgatg tcggtgtgga
    17401 tctgaccggc ggctggtatg atgctggaga tcatgtgaaa tttggttttc caatggctgc
    17461 ctcggcaacc atgctggctt ggtccgtcgt cgagtatagt gatggctatg agcaagccgg
    17521 acaactggag gaaatcaagg acaatataag gtgggcaacc gactatttta tgaaagcgca
    17581 taccaaacca aacgaattat ggggacaagt cggaggtggc aacacggatc atgcttggtg
    17641 ggggcctgca gaggtgatgc agatgaaccg gccttcgttc aagatcgatg cttcctgtcc
    17701 gggaagtgat ctggcggcag aaacggcggc tgcactggca gcggcgtcca tcgtatttgc
    17761 agatgatgat ccaacctact cggcgaagat gcttcagcat gcgaaagaac tgtacaactt
    17821 tgcagataca tatcggggga aatacaccga ttgtattact gatgctgctg cattttataa
    17881 ttcgtggacg ggatatgaag atgagcttgc atggggagga gcatggcttt atttagctac
    17941 taatgataac gcttacttgt ccaaggcgat ggcagctgca gatcggtggt ctacaagcgg
    18001 aggttccgcg aactggccct atacctggac acagggctgg gatagtaaac attacggtgc
    18061 ccagattctg cttgcccgaa tcacctccag tttgaacatg ccggaggcaa caaagttcat
    18121 ccaatcaacc gagcgcaatc ttgattactg gacggttggc acgaatggag ggcgtgtgac
    18181 ttacacccca ggcggattgg cgtggctgga tcagtggggt tcactccgat atacggcaaa
    18241 tgcagcattc attgcattcg tgtactccga ttgggttagt gaccctgtga aaaaatccag
    18301 atatcaaaat tttgcgactt cacagatgaa ttacatttta ggagataatc ctcgtcagag
    18361 cagttatgtt gtgggatatg gaaaaaatcc gccacagcat ccgcatcatc gtaccgctca
    18421 tagctcatgg atgaacaacg aagatattcc ggcgaatcac cgtcatattt tatacggcgc
    18481 catggtagga ggaccaaatg cgtcggatca atatacggac gatatctcgg actatgtgag
    18541 taatgaggtg gcgacggatt ataatgctgg cttcacaggt gcgctcgcaa agatgaatct
    18601 gctgtatgga cagaatcatc agcctctggc gaatttccct gctcctgaag tcaaaggtga
    18661 ggagtacttt gtagaagctg ctgtgcggtc atcaggctcc aattatacgg aaatccgcgc
    18721 attgcttaac aaccgctccg gctggcctgc acgaatggga gatcagcttt ccttcaaata
    18781 tttcctcgat ttgagtgaag tatatgctgc gggacgcacc gtatccgatg ttcaggtcac
    18841 agtatcttct aatgaaggtg caaccgtttc tcaacctgtc gtcgtggatg ccgccaagcg
    18901 gatctatgcc attacagcag acttcagtaa taccaaaatt tatccaggcg gagaggggaa
    18961 ttaccgcaag gaagttcagt tccgcattac aggaccgcaa ggggcatgga atccagcgaa
    19021 cgatccttca taccagaacc tgacgacagg tactccggta aaaagtgtat acatccccgt
    19081 atatgacgct ggagtgaagg tatatgggca ggagccgggt gttacacctg tgaccgtgcc
    19141 agccgcaccc tctggagtgc aagctttgcc tggcaacaat caggtgacgc tgaactgggc
    19201 tgcctctgcc ggggctgtat cgtacacggt gaagcgtgcc gaggttagcg gaggcccgta
    19261 cacaaccgtt gcaacgggtg ttaatggggt gacttacacc aacacgggat tgacgaatgg
    19321 caagacgtat tattatgtgg tgaccgctgt taatgcaggc ggggaatcgc cagcttctgt
    19381 tcaagtgatg ggaacgccgc aggcagtttc atctgtgcca gggacgctta ccttaagcgg
    19441 aacagctggc aataatcaaa atgtgttgtc atggacagcg gcatcggggg ctgcgagtta
    19501 caaggtacaa cgtgctaatg cgaatggtac gtatgcggat gtagcgaccg ggttgacggt
    19561 gatgacctac actgacacga cagcgcttaa tggaacttcg tataattacc gggttgttgc
    19621 cgtaaacgca agtgggcaat cgttatctaa cgtggttgta ctgacacctt cagggccgcc
    19681 tgtgtcaaca ggtacgctgg aagtgcagta tcgaagcgga ggttccggga attcgagcaa
    19741 tgcggtaacc cctcagttta atgtgaagaa cacaggcaca cagcctattg atctgagtac
    19801 ggtaaaaatc cgctactact ttaccaaaga tggcacagaa gatctgacct tctggtgtga
    19861 ttacgccgtg gtgggtacgg ctaacgtaca agggaagttc gtgaccgtga atccggcaaa
    19921 aggaacggcg gatacgtatc tggagatcag ttttacctct ggagcgggaa gtttggcagc
    19981 cggagcagaa acgggtgtga ttcagggacg tttttcaaaa aacaactgga gtaacttcga
    20041 tcagagcaat gattactcct atgacggttc caagactgct tttgctgcat ggaacaaagt
    20101 gaccgcgtat caaggcagta cccaggtatg ggggctggag ccgtaaatca aaactttgac
    20161 agaagaggcc aaagaacagc atgggcagac cagcagcatt gatattgaat ttccactatt
    20221 ccagggaggt atatattcat gttgaa
//
