LOCUS       NZ_CP023865            11964 bp    DNA     circular CON 27-APR-2024
DEFINITION  Actinoplanes teichomyceticus ATCC 31121 chromosome, complete genome.
ACCESSION   NZ_CP023865
VERSION     NZ_CP023865.1
KEYWORDS    RefSeq.
SOURCE      Actinoplanes teichomyceticus ATCC 31121
  ORGANISM  Actinoplanes teichomyceticus ATCC 31121
            Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
            Micromonosporaceae; Actinoplanes.
REFERENCE   1  (bases 1 to 8287152)
  AUTHORS   Yushchuk,O., Horbal,L., Fedorenko,V., Ostash,B., Luzhetskyy,A.,
            Kalinowski,J. and Ruckert,C.
  TITLE     Complete genome sequence of Actinoplanes teichomyceticus ATCC 31121,
            type strain of Actinoplanes teichomyceticus and producer of
            teicoplanin, isolated from soil near Nimodi, India
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8287152)
  AUTHORS   Yushchuk,O., Horbal,L., Fedorenko,V., Ostash,B., Luzhetskyy,A.,
            Kalinowski,J. and Ruckert,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2017) CeBiTec, Bielefeld University,
            Universitaetsstr. 27, Bielefeld, NRW 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8; canu v. 1.5
            Genome Coverage                   :: 184.1x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003711105.1-RS_2024_04_27
            Annotation Date                   :: 04/27/2024 03:18:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,294
            CDSs (total)                      :: 7,220
            Genes (coding)                    :: 7,116
            CDSs (with protein)               :: 7,116
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 27 of 104
            Pseudo Genes (incomplete)         :: 92 of 104
            Pseudo Genes (internal stop)      :: 11 of 104
            Pseudo Genes (multiple problems)  :: 22 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:55:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4856205
            Orig. end                         :: 4868169
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023865.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11964
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="17"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..11964
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11964
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:6964]"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6964
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    132..147
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.27"
                     /tool="antismash"
     misc_feature    908..921
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.94"
                     /tool="antismash"
     gene            <1346..2182
                     /locus_tag="ACTEI_RS21450"
                     /pseudo=""
     CDS             <1346..2182
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (22-257). E-value:
                     1.6e-36. Score: 118.1. Matches aSDomain:
                     nrpspksdomains_ACTEI_RS21450_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S9"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018812057.1"
                     /locus_tag="ACTEI_RS21450"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /pseudo=""
                     /sec_met_domain="PF00561 (E-value: 3.9e-27, bitscore: 94.2,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 6.1e-18, bitscore:
                     65.0, seeds: 455, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_S9 (E-value: 4.1e-06, bitscore:
                     25.1, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="SMGYVTTSDGTNIYVKDWGAGRPVVLSHGWPLNSDSWEAQQLFLA
                     QNGYRVIAHDRRGHGRSDQTWHGNEMDTYAADLAAVIETLDLRDVTLVGFSTGGGEIAR
                     YIGNHGTARVAQAVLVSAVPPLMLQTDDNPGGLPISVFDGIRAGSLADRSQLYKDFAAG
                     PFFGANRPGAKVSQGMQDSFWLQGMTAGHHNTYECIAAFSATDFRGDLAKFDIPTLVIH
                     GDDDQVVPFEVGGKASAALIKGAELKVYAGGPHGITDTHKEQLSEDLLTFLNSYAA"
     aSDomain        1415..2119
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ACTEI_RS21450_Abhydrolase_1.1"
                     /evalue="1.60E-36"
                     /label="ACTEI_RS21450_Abhydrolase_1.1"
                     /locus_tag="ACTEI_RS21450"
                     /protein_end="257"
                     /protein_start="22"
                     /score="118.1"
                     /tool="antismash"
                     /translation="PVVLSHGWPLNSDSWEAQQLFLAQNGYRVIAHDRRGHGRSDQTWH
                     GNEMDTYAADLAAVIETLDLRDVTLVGFSTGGGEIARYIGNHGTARVAQAVLVSAVPPL
                     MLQTDDNPGGLPISVFDGIRAGSLADRSQLYKDFAAGPFFGANRPGAKVSQGMQDSFWL
                     QGMTAGHHNTYECIAAFSATDFRGDLAKFDIPTLVIHGDDDQVVPFEVGGKASAALIKG
                     AELKVYAGGPHGI"
     misc_feature    3077..3092
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.86"
                     /tool="antismash"
     misc_feature    complement(3079..3092)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.74"
                     /tool="antismash"
     misc_feature    complement(3080..3095)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.35"
                     /tool="antismash"
     misc_feature    complement(3143..3158)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 16.33"
                     /tool="antismash"
     misc_feature    complement(3163..3176)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.97"
                     /tool="antismash"
     misc_feature    3185..3200
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    3267..3277
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     gene            3316..3711
                     /locus_tag="ACTEI_RS21455"
     CDS             3316..3711
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014691975.1"
                     /locus_tag="ACTEI_RS21455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_122979279.1"
                     /transl_table=11
                     /translation="MRRTHYAAVLAGVAAALAPSAVQAAPATTTGLLGTYDFGRAEVIG
                     QAGTEAGVWRSFTVRHGFAGDDLTVTYTPRTEPYRTERLVYTAAFDAVADPQAECREVG
                     ADGVARQVWVSFRCQTGFVPSYTLYVR"
     misc_feature    3729..3744
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.26"
                     /tool="antismash"
     misc_feature    3909..3923
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.52"
                     /tool="antismash"
     misc_feature    complement(3931..3944)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.66"
                     /tool="antismash"
     gene            3954..4388
                     /locus_tag="ACTEI_RS21460"
     CDS             3954..4388
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ACTEI_RS21460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_145830879.1"
                     /transl_table=11
                     /translation="MKKSTISALGVLAGVGAVLAMGAPAAAADHTFDLATGGAAAYGTY
                     DRMMSIPERPVPPIRVQGTLALNGRNRCAVVQIAANGPADGIEWRTLNSLCQPGKTNFS
                     AASDYLWGGFEPGLRLCVGATVQRAEQGRDCDVHTPAATS"
     misc_feature    complement(3958..3972)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.8"
                     /tool="antismash"
     misc_feature    complement(3970..3985)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.03"
                     /tool="antismash"
     gene            5001..5447
                     /locus_tag="ACTEI_RS21465"
     CDS             5001..5447
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF017995.4"
                     /locus_tag="ACTEI_RS21465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_122979281.1"
                     /sec_met_domain="melC (E-value: 5.1e-15, bitscore: 54.4,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSHLSRRAVLRYAAAATAVTGGVVGAQALSAAGSPPAAAASTDPR
                     DFELDYRGKKIKGVHGIGTGRSRQPHQVYINGRKLAVMAIELPATDGTGVSVGFISALN
                     HYQPVLIDEGENRDGLLVLARRAVDALGDGELTDLAGADHDHGR"
     gene            5495..6316
                     /locus_tag="ACTEI_RS21470"
     CDS             5495..6316
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014691977.1"
                     /locus_tag="ACTEI_RS21470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase family protein"
                     /protein_id="WP_122979282.1"
                     /transl_table=11
                     /translation="MGLRKSARDLTSAERAAFVSAVKRLKAQTTGRNYDWFVTTHLNYF
                     AAVNGIRYAHQSPSFLPWHRQYLIEFERALQVYDPTVTVPYWDWTADRSTTGTPFTSDF
                     MGGNGSGGNGPVTTGPFAGATNWRITVSSTTSTSLRRAMGGTTLPSATQVSSVLGIPAY
                     DAAPWNSSATGGMRNRMEGWTTPNLHNTVHVWVGGHMGQQDSPNDPVFWLHHCNVDRLW
                     SQWQVRWGFDAAHYLPAAGTSGVVDLDEALQPWPDVTPASVLDHRSVYTYA"
     misc_feature    complement(5543..5554)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
     gene            complement(6641..6964)
                     /locus_tag="ACTEI_RS21475"
     CDS             complement(6641..6964)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF017995.4"
                     /locus_tag="ACTEI_RS21475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_164466036.1"
                     /sec_met_domain="melC (E-value: 5.1e-14, bitscore: 51.1,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAHAPVVAHAPAVAHAPAGMVADTYARAGAFAEWYQGRYIRGWGG
                     AQPCAFIDGVSLGLVDHGGNRYSSVVNAYEITTGVRATTRAAVRSLAGAGLQPTEPPAY
                     CRR"
     misc_feature    7135..7148
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 19.79"
                     /tool="antismash"
     gene            complement(7157..7534)
                     /locus_tag="ACTEI_RS21480"
     CDS             complement(7157..7534)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /locus_tag="ACTEI_RS21480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_239082626.1"
                     /transl_table=11
                     /translation="MAFAFTLAGMQLSPGRLALGLLGFNLGIEVVRLLLIGLVLTAAAG
                     SAGLRRPCGGPSPEPCGRARAPVAGRWRPAARDRALVVVRCLRSGGGWVLVVVRCLGSG
                     GGRAPVTVRCSGSRLVRSGMP"
     gene            complement(7498..8622)
                     /locus_tag="ACTEI_RS37225"
     CDS             complement(7498..8622)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ACTEI_RS37225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_164466038.1"
                     /transl_table=11
                     /translation="MTTRTSPRRPPRRRGSRTGRAWRRADAFPAALNSDQKALAQLELT
                     RQRAPARSNLSCGATCRPAVMVGGLDDAQGAAGRSVLLTALGTGSGTGFERVVEIFAAD
                     ARLGASPPAISRRPVACHRRTPAGCATTWPHTCAPVSAGDRRWRVVIGRVSRARTSGPR
                     PGRTARWSPPRRSPRRPARTCGASGSGTTPWCTGGATHAVLVTVRRDWAAGRVGPDRDA
                     VTAATIATDTRTMTVRDLTVDLGGSTWRGFLAMVRLGGQHILAGTDHLLFPLVLLLPVT
                     LRARAGRWRGPSRPPSGGRDLRLHGRARVSLAVCVLGRARFPAAPVEVFIAASILVGAL
                     HAIRPLFPTGRPPWPACSASATGWRSRSRSPVCS"
     gene            complement(8768..9553)
                     /locus_tag="ACTEI_RS21485"
     CDS             complement(8768..9553)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1207: inositol monophosphatase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014692172.1"
                     /locus_tag="ACTEI_RS21485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="inositol monophosphatase family protein"
                     /protein_id="WP_122982317.1"
                     /transl_table=11
                     /translation="METDLELAQRAALTGAAEALRHFAVIADLPQERKQDGSVVTLADR
                     AVEHAIRDVLTDARPDDAILGEEHGETGGGNGRRWIIDPIDGTALFVRGDPRWLVLLAL
                     EVHGEVTVGVQVVPAQGLAWWAQRGQGAYRGRVSGGALVGAERIHVAAVPAGAAGSRLG
                     VVPLDWGREVAEPLIAVAAEQHWPVHPPLLVAQGELDVAVQTSGQIWDFAASSVIVPEA
                     GGCYRGLDGQERPGAGAFVFAADPRLLDESLRILRGVPK"
     misc_feature    complement(8795..8797)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(9612..9629)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.61"
                     /tool="antismash"
     gene            9641..10357
                     /locus_tag="ACTEI_RS21490"
     CDS             9641..10357
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014692173.1"
                     /locus_tag="ACTEI_RS21490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_122979285.1"
                     /transl_table=11
                     /translation="MGPPVPDRIASGVVFTTPASLPRGRHELSRAQVVAAQRERMLIAA
                     TELLAGRGYRGFGVREICARAAVSRAAFYDCFADKDACVYAAYDRFIEVFLERLADDAG
                     TAGDWAGVVGGFVTAYLATLERDRVTARAFQVEMDALGRPARQRRRQAIERLAGFVHDM
                     YERRFAGDAPGAPLSAYIGAIYALRQIASDALDSGDSPDLRSLAPELTAWLSRMIVTDS
                     TGRPPAAPPEEGSPTR"
     misc_feature    complement(9663..9678)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.22"
                     /tool="antismash"
     misc_feature    11090..11102
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 16.34"
                     /tool="antismash"
ORIGIN
        1 gtcccacgcg cagctgagca aaccggcccg ggagctgttc cggcggctcg cgcacgtgcc
       61 cggggtggac ttcgcggcca ccctcgcggc ggtgctcacc ggcggtcacc cggacgacac
      121 cgcggacggg ctggacgagc tggtcgacct gggcctgctg caacagtccg gaccggaccg
      181 ctaccgtttc cacgacctga tccggctgtt cgccgaggaa cggttgcgga tcgaggagcc
      241 ggccgggacc cgcgcggcga ccgccaagaa gatgtccgac tggctgctgg agacggcgat
      301 cgtggccggc cgctggttcg agcccgggta cggccgcctg cccgacggct ggggcgggcc
      361 ggtcgcgctg ggcaccgccg aggaggcgga cacctggctg caggcggagc gggcgaactg
      421 gctcggcgcg atgcgctcgg cggcccgcgg cgggcagtac cagctggtcg tcgacgtggc
      481 cgaggcgatg cactggtact ccgacaagta cgtgcgcgcc gactactggt acgaggtgta
      541 cggcatgtcc cgggccgccg cggcccgact gccggaccgg cggcaggagg cgacgcacct
      601 caactacttc tcctgggcgg cgacgcactg cgcccggcgt ccgggcgagg gcgccgagat
      661 cgcgatggac gcctaccgga tcgcggccgg gctcggcgac ctcaaggagc agtcctgggc
      721 gctgcgctac gccggcgacg cctggcggca cggcgggatg ccggaacggg cgctcgacgc
      781 ctaccggcgg gccaccgagc tggccgaccg ggccggcgac cacgacggct acatccagct
      841 gcggcccggc atcgcccggg cgctcatcgc gctgggccgg cacgaggagg cgatcgagga
      901 gtgcgccgcg acgctgcgcg acatcgagac ccgtccggtg gcggcccgac cggagctgag
      961 cgcgcgggtc agcgcgcacg gcatcgcggc cgagtcactc gccgagagcg gccggtgggc
     1021 ggaggcgctg cgcgaggcgg agcgggcgat gccgtacgcg gtcgagttcg gctaccccgg
     1081 ctacctcggc gagctgcacc tgacgatcgg ccgggcccgg atggcgctcg gcctgcgtga
     1141 cgcggcccgg ccggcgctgc gcaaggccgt cgagctgctc gacgatctgc cgcaccagcg
     1201 ctggcggcaa ctggcccggc aggggctggc cgcgctcggc gagaccgcat gaggttctgc
     1261 gtatcgttct gcgtggtcgc ccacccgccg cggtgattgc atgggggtac ggccgcgcca
     1321 ccggagcgcg ccgcgggaag ggacctccat gggctacgtg accacctccg acggcacgaa
     1381 catctacgtc aaggactggg gtgccggccg cccggtcgtg ctcagccacg gctggccgct
     1441 gaactccgac agctgggagg cgcagcagct gttcctggcc cagaacggct atcgggtgat
     1501 cgcgcacgac cgtcgcggcc acggccgctc ggaccagacc tggcacggca acgagatgga
     1561 cacctacgcc gccgacctgg ccgccgtcat cgagaccctc gacctgcgcg acgtcaccct
     1621 ggtcggcttc tccaccggag gcggcgagat cgcccgctac atcggcaacc acggcaccgc
     1681 ccgggtcgcg caggccgtgc tggtctccgc ggtgccgccg ctgatgctgc agaccgacga
     1741 caacccgggc ggcctgccga tctcggtgtt cgacggcatc cgcgccggct cgctcgccga
     1801 ccgctcgcag ctgtacaagg acttcgcggc gggcccgttc ttcggcgcga accggccggg
     1861 cgcgaaggtc tcgcagggca tgcaggactc gttctggctg cagggcatga ccgccgggca
     1921 ccacaacacc tacgagtgca tcgccgcctt ctcggccacc gacttccgcg gcgatctggc
     1981 caagttcgac atcccgacgc tggtgatcca cggtgacgac gaccaggtcg tgccgttcga
     2041 ggtgggcggc aaggcctcgg ccgcgctcat caagggcgcc gagctcaagg tgtacgccgg
     2101 tggcccgcac ggcatcaccg acactcacaa ggagcagctg agcgaggacc tgctcacgtt
     2161 cctcaacagc tacgccgcct gacgccggcc ggtgcgggga ggcgatgggc ttccccgcac
     2221 gccggccacg cgaggaggcg agcacaggta acaccatgga cacggacaca ggctcgccgc
     2281 gcgagcgcgc cgtccactga agcgcctcgg cgccggctcc tcgccgcgag cggtccccgc
     2341 gaccgtccgt cccggcgcga ggagctcttt cgcatgtcca aaccgcgccc cgggcgcgga
     2401 gttggcacgt ctccggagcg ccacggcggt ctccagggcg gccggagcgc cgctgaacgg
     2461 cgacccggcg ccgcgcccgg agcgtcgcgg aagccgcgtg tgcccggtcc ggccggcacg
     2521 cgccgcttcg tccagcagcc ggacccgtgc cgtcgcgccc gccagccgaa cctgcgccgt
     2581 ccgcccagca gccagccccg cgccgtcgcg cccgccagcc ggacccgcgc cgtcccgccc
     2641 agcagccgga cccgcgccgt cccgcccgcc agccgacccg cgccgtcccg cccagcagcc
     2701 agccccgcgc cgtcgcgccc gccagccgac ccgcgccgtg aggccccgcg gcagacagcc
     2761 caccggccgg gcacgcgccg agcacccggc cccctgccgc cctgccgcac ccggcctctt
     2821 gccgcccagc tcagcgcccg gccccctgcc gcccagctca gcacccggcc tctgccgccc
     2881 tgccgccctg ccgccggacc atgccgcccc gcccggcacc cgggcgcggc cgcctcgccg
     2941 agcggccagc cgcccgcacc gcaccgccca ccggccgggc acgcgcgccc tgaggcccgg
     3001 cgcctcgacg gccgcccggc gcgccggaat acccggcata tccccgccaa cgcgcgatac
     3061 cggagaccgg gtgacaattg ctgctgatta tcactgcaag cgattgccta tgtttttccg
     3121 ccatccaacc cggaagcctc gacttaacaa tcgttaattg cgtacgaata gttatccctt
     3181 ccactgtgac ctcatccaca tcgtccagta tggaatggtg atatggttcc gtcgcgccgg
     3241 tagggagaac ccttcaccgg tccatctgca atgccacggg aagatccccg gggccaaggg
     3301 caaaggaagt taaaaatgcg cagaacccat tacgccgccg tcctggctgg cgtggccgcc
     3361 gcgctcgccc cgtccgccgt tcaggcagca ccggccacca caaccggcct gctcggcacg
     3421 tacgatttcg gccgcgccga agtgatcggc caggccggga cggaggccgg agtgtggcgg
     3481 tcgttcaccg tgcgccacgg cttcgccggt gacgacctga ccgtcacgta cacgcctcgc
     3541 accgagccgt accgcacgga gcgactggtg tacacggccg ccttcgacgc ggtcgccgat
     3601 ccgcaggccg agtgccgtga ggtcggcgcg gacggcgtgg cccgccaggt gtgggtgtcg
     3661 ttccgctgcc aaaccggctt cgtgccgagc tacacgctct acgtgcgctg acacttccgg
     3721 ccattcggcc gaggtttctt ttaccgggcg ggaagcccgt tccgcgattc cgccgtgcgg
     3781 tcgcgcgtcg atccaccgga tcacgcgtcg agaggcccat cgccacggcg tcgtcacgcc
     3841 gtaaccgaat cacgggcccg cccgtcacgc gaccaccttt tccggccccg ccaccggtgg
     3901 ccggctgccg catgtcattt tccaccggca gaataacaat tctggagttt acgatgaaga
     3961 aatcgactat cagcgctctc ggcgtcctgg cgggcgtcgg tgccgtcctc gcgatgggcg
     4021 cacccgccgc cgccgcggac cacaccttcg acctggccac gggcggcgcc gccgcgtacg
     4081 gcacctacga ccggatgatg agcatcccgg agcggcccgt cccgccgatc cgggtgcagg
     4141 ggacgctggc gctgaacggc cgtaaccggt gcgccgtcgt ccagatcgcc gccaacggcc
     4201 cggccgacgg catcgagtgg cgcaccctca acagcctgtg ccagcccggc aagaccaact
     4261 tctcggccgc ctccgactac ctctggggcg gcttcgagcc cggcctgcgg ctgtgtgtcg
     4321 gcgccaccgt gcagcgcgcc gagcagggcc gcgactgcga cgtccacacc cccgcggcga
     4381 ccagctgatc gcgctgcggc ggcaccgaag gcaccgctga aggcaccggt cacaggccgg
     4441 cacgacccgt cccgatcacc cgtccactga tccggccggg tcgtgccgcc tcgccggccg
     4501 tcaccggagc ggccagcccc gcgggcctgt cgccaccgga accggccatc accggaagca
     4561 cccaccagcg gagcccagcc accaccggaa gcacccacca gcgaagccca gccaccaccg
     4621 gaagcaccca ccagcggagc ccagccacca ccggaagcac ccaccagcgg agcccagcca
     4681 ccaccggaag cacccaccac cggaagcacc caccagcgga gcccagccac caccggaagc
     4741 acccaccagc ggagcccagt cgccatcggg tccggctcca ccggaaccac ccaccccgga
     4801 gccgggcacc accggaaccg gccgctccac taccgacgaa ccggccgctc cactcgggcg
     4861 atccggccgc tccactaccc ggcgaaccgg ccgctcccct gccggctcgt ccggtgtccc
     4921 gcacgggtgt cgcgggacac cggttcatcc cgccgccgcg cgttccgcgg cggcggccac
     4981 ccaccggaag aggcacatac atgtcccacc tgagtcgtcg agccgtcctc cgctacgcgg
     5041 ccgccgccac cgcggtcacc ggcggtgtcg tcggcgcgca ggccctctcc gccgccggca
     5101 gtccgccggc cgccgcggcc agcacggatc cgcgcgactt cgaactcgac taccgcggca
     5161 agaagatcaa gggagtgcac ggcatcggta cggggcgcag ccgccagccg caccaggtgt
     5221 acatcaacgg gcggaagctg gccgtcatgg cgatcgagct gcccgccacc gacggcacgg
     5281 gcgtctcggt cggcttcatc agcgccctca accactacca gccggtcctg atcgacgagg
     5341 gcgagaaccg tgacgggctg ctggtgctcg cccggcgggc ggtcgacgcg ctcggcgacg
     5401 gcgagctcac cgatctggcc ggcgcggacc acgaccacgg gcgctgaccc ggccaccgac
     5461 cccctctcga ccgatcgacc agaagggcac cccgatgggc ctccgcaaga gcgcgcgcga
     5521 cctgaccagc gcggagaggg cggcgttcgt ctccgccgtc aaacgcctca aggcgcagac
     5581 caccggtcgt aactacgact ggttcgtcac cacccacctg aactatttcg ccgccgtcaa
     5641 cggcatccgc tacgcccacc agtccccctc gttcctgccg tggcaccggc agtacctcat
     5701 cgagttcgag cgggcgctgc aggtctacga ccccacggtc accgttccct actgggactg
     5761 gacggcggac cgctcgacga ccggcacgcc gttcacgtcc gatttcatgg gcggcaacgg
     5821 ttccggcggc aacggcccgg tcaccaccgg accgttcgcc ggcgccacca actggcggat
     5881 caccgtcagc agcaccacct ccacgtcgct acggcgcgcc atgggcggca ccaccctccc
     5941 gagcgccacg caggtgagct cggtgctcgg catcccggcg tacgacgcgg ccccgtggaa
     6001 cagctcggcg accggcggca tgcgcaaccg gatggagggc tggacgacgc cgaacctgca
     6061 caacacggtg cacgtctggg tcggcggcca catgggtcag caggactcgc cgaacgaccc
     6121 cgtcttctgg ctgcatcact gcaacgtcga ccggctgtgg tcgcagtggc aggtgcggtg
     6181 ggggttcgat gccgcgcact acctgccggc cgccggcacc agtggtgtgg tggacctcga
     6241 cgaggccctg cagccgtggc cggacgtgac cccggcatcg gtgctcgacc atcgaagcgt
     6301 ctacacgtac gcctgatccg ccggccggtc gcggccgccg cctgatccgg cgcccgcgac
     6361 cggcccccgc tacgcccgcg acccaccgcg cacccaacac ccgcgctacg cccccgaccc
     6421 accgcgcacc caacacccgc gctacaaccg cggcccaccg cgcacccaac acccgcggta
     6481 caaccgcgac ccaccgcgca cccgaccaac cgcgaccgac cgcccaccca acaaccgcga
     6541 cccaccgcgc agcccaccca accgcgaccc accacccact caacacccgt ggtacgaccg
     6601 cgacccaccg cccagccaac aaccgcggcc gaccgcccac tcaccgccgg cagtacgccg
     6661 gcggctcggt cggctgcaac ccggcgccgg ccaggctgcg caccgcggcc cgggtcgtcg
     6721 cgcggacgcc ggtggtgatc tcgtaggcgt tgaccacgct gctgtagcgg tttccgccgt
     6781 ggtcgacgag ccccagggac acgccgtcga tgaaggcgca cggctgcgcg ccgccccagc
     6841 cgcggatgta ccggccctgg taccactcgg cgaaggcgcc ggcccgggcg taggtgtccg
     6901 ccaccatccc ggccggcgcg tgggccacgg caggcgcgtg ggccaccaca ggcgcgtggg
     6961 ccacggcagg cgcgtgggcc acggcaggcg cgtgggccac ggcaggcgtc gtggcgagga
     7021 cgcagattcc ggcaggcagc agggtcgcgg tcaggacagc ggttgccagg cgtcgcatca
     7081 tcggtgtctc ccggtcagaa agttaatcga cggtgctcaa tgtattccac atcgtggacc
     7141 ggatccaccg cgcacgctac ggcataccgc tccgaaccag gcgcgacccg ctgcaccgga
     7201 ccgtgaccgg cgcccgccca ccgcccgaac ccaggcagcg gaccacgacc agcacccacc
     7261 caccgcccga tcgcaggcag cggaccacga ccagcgcccg atcccgggca gccggccgcc
     7321 accggcccgc gaccggcgcc cgagctcgcc cgcagggctc aggtgacggg ccgccgcacg
     7381 gccggcgcaa gcccgcgctc ccggccgccg cggtcagtac caggccgatc agcagcagtc
     7441 gcaccacctc gatgcccagg ttgaagccga gcagcccgag ggcgagccgg ccggggctca
     7501 gctgcatacc ggcgagcgtg aacgcgaacg ccatcccgtg gccgaggccg aacaggcagg
     7561 ccacggtggc ctcccggtcg ggaagagtgg ccggatcgcg tgcagcgcgc cgaccaggat
     7621 gctcgccgcg atgaacacct ccaccggcgc cgccgggaac ctcgcccggc ccagcacgca
     7681 gaccgccagg gacacccgtg cccgaccgtg aaggcgcagg tcacggcccc cgctcggcgg
     7741 gcggctcggc ccgcgccagc ggccggcgcg ggcccgcagc gtcaccggca ggagcaggac
     7801 cagcgggaac aacaggtgat cggtgcccgc caggatgtgc tggccaccga gccggaccat
     7861 cgccaggaag ccgcgccagg tgctgccgcc gaggtcgacg gtcaggtcgc ggaccgtcat
     7921 cgtccgggtg tccgtggcga tcgtcgcggc cgtgacggcg tcccggtccg gcccgacccg
     7981 cccggccgcc cagtcgcgcc gcacagtgac cagcacggcg tgcgtggcgc ccccggtgca
     8041 ccacggcgtc gtacccgagc cggaagcgcc gcacgtccgc gccgggcggc ggggtgaacg
     8101 tcgcggtggc gaccatctcg cggtacggcc cggtcgtggt ccgctcgtcc gcgcccggct
     8161 cacccgcccg atcaccaccc gccagcgccg gtcaccggcc gacaccgggg cgcaggtgtg
     8221 cggccaggta gtcgcgcacc cggccggcgt ccgccggtgg cacgccactg gccggcgtga
     8281 aatcgcgggc ggggatgcgc ccaggcgcgc gtcggccgcg aagatctcga cgacccgctc
     8341 gaacccggtg ccggagccgg tgcccagggc ggtgagcagc acgcttctac cggctgcccc
     8401 ctgcgcgtcg tcgaggccgc cgaccatcac tgcgggccgg caggtggcgc cgcacgacag
     8461 gttcgaccgg gcgggcgccc gctgccgggt gagctccagc tgggccaggg ccttctggtc
     8521 gctgttcagg gcggccggga aggcgtcggc ccgccgccat gctcttcccg tacgacttcc
     8581 ccgtcgccga ggtggccggc gtggcgacgt cctcgtcgtc atcggggggc cgccgtcgca
     8641 ccgagcggca gggcggcggc gccggtgcga gcggccccgc ggcgggcggg tgcggcgatc
     8701 acgaaccact tcattcgtac ggaagcagat ccggatcaga acaacgaccc cttaccgagt
     8761 catgtggtta tttgggaact ccgcgaagga tgcgtaacga ttcgtcgagc agccgcggat
     8821 cggcggcgaa cacgaacgcc ccggcacccg gacgctcctg cccgtccagg ccgcgatagc
     8881 agcccccggc ctccgggacg atcaccgagc tggccgcgaa gtcccagatc tgcccgctgg
     8941 tctgcaccgc cacgtccagc tccccctggg cgaccagcaa cggcggatgc accggccagt
     9001 gctgctcggc ggccaccgcg atcagcggtt cggcgacctc ccgcccccag tccagcggca
     9061 ccaccccgag ccggctcccg gccgccccgg ccggcacggc ggccacgtga atccgttccg
     9121 cgccgaccag cgcgccgccg gagacccgcc cccggtacgc cccctgcccg cgctgcgccc
     9181 accacgccag cccctgggcc ggcaccacct ggacgccgac cgtgacctcg ccgtgcacct
     9241 cgagcgcgag cagcaccagc caccgcggat cgccccggac gaacagcgcc gtcccgtcga
     9301 tcgggtcgat gatccaccgc cggccgttgc cgccgccggt ctcaccgtgc tcctcaccga
     9361 ggatcgcgtc gtccggccgg gcgtcggtga gcacgtcgcg gatcgcgtgc tcgaccgccc
     9421 ggtcggccag ggtcaccacg ctgccgtcct gcttgcgctc ctgcggcaga tcggcgatca
     9481 ccgcgaagtg gcgcagcgcc tcggcggcgc cggtcagtgc ggcccgctgg gccagttcca
     9541 gatccgtctc cacgccgacc atcctgccgg gccggcggcg aggacttgtc gggtgacgcg
     9601 ggaacgccgt gggaagattg gagtaacaaa gcgtactcag atgggaccgc ccgtgccgga
     9661 ccggatcgcc agcggcgtcg tcttcacgac ccccgcgtcg ctgccgcgcg gccgccacga
     9721 gctcagccgc gcccaggtgg tcgccgcgca gcgggagcgg atgctgatcg cggccaccga
     9781 gctgctcgcc ggtcggggtt accgcggttt cggggtgcgc gagatctgcg cgcgggcggc
     9841 ggtgtcccgg gccgcgttct acgactgctt cgccgacaag gacgcctgcg tctacgccgc
     9901 gtacgaccgg ttcatcgagg tgtttctgga gcggctggcc gacgatgccg ggacggccgg
     9961 cgactgggcc ggcgtggtcg gcggcttcgt cacggcgtac ctggcgaccc tggaacgcga
    10021 ccgggtcacg gcccgcgcct tccaggtgga gatggacgcg ctggggcggc cggcccggca
    10081 gcggcgccgc caggccatcg aacggctcgc cggtttcgtc cacgacatgt acgagcgccg
    10141 gttcgccggc gacgcgccgg gcgccccgct gagcgcctac atcggcgcga tctacgccct
    10201 gcgccagatc gcgtcggacg ccctggacag cggcgactca ccggacctgc ggtcgctggc
    10261 gccggaactg accgcctggc tgtcccggat gatcgtgacc gacagcaccg ggcggccgcc
    10321 cgccgccccg cccgaggaag gcagccccac ccgatgacgc agacctgcac cgcgcccccg
    10381 gccgaggaag ccgtcctggc cgagctgcgc gagcacgcgc cggcctcgtt cggggcgatg
    10441 ttcctggacc ggacggccgc gcagccgcgc gaggtggcct acctgcgccc cggaccggac
    10501 ggctggcaga gccggacctg ggcgcagacc gcgcaggcgg tcaccgagat cgccgccggt
    10561 ctgctggccc tcggtctgcg gccggaggac cgggtcgcga tcgccgccgg cacccgggtg
    10621 gagtggatcg aggcggactt cggcgtgatg tgcgccggcg gcgccaccac cacggtctat
    10681 ccgtcctcct cgccggagga ggtcgcgcac atcctgaccg actcgggcag ccggttcgcc
    10741 gtcgccgagg acccgtcgca cctcaccagg ctgctgttcg ccggcacgcc ggtggagcag
    10801 gtggtgctga tcgacggcga cgccccggac gacccgcggg tgcgcaccct cgccgcgctg
    10861 cgcgcgctcg gccacgacga gctgcgtgag cggcccggcc tcgtcgagga cgccaccgcc
    10921 cgggtacgac ccgagcacct ggccaccctg atctacacct ccgggaccac gggcctgccc
    10981 aagggcgtcc gggtcgcgca ctccgcctgg atctatcagg gcctggcgat ccgggcgatg
    11041 ggcatcctgc actccgacga cctggcgtat ctctggctgc cgctgtccca cgcattcggc
    11101 aaggcgctgc tgagctgcca gctgtcggtg ggcttcccgt tcgcggtgga cggcgacgtc
    11161 agccggatcg tgcagcggct cggcgaggtg cggccgacga tcatgcccgc cgtaccgcgg
    11221 atcttcgaga aggtctacgc cgcggtgagc gccgccaccg gcgtacagcg aaggctgttg
    11281 aactgggcga tcggcgtcgc cgaccggcgc cggtcgggcc tgcgctaccg gatcgccgac
    11341 cgcctggtcc tggcgaaggt ccgggcccgg ttcggcggcc ggatgcggtt cttcatctcc
    11401 ggcgccgccg cgctctcccc ggacatcgcc cgctggttcg acgcgatcgg cctgccgatc
    11461 gccgaggggt acggcctcac cgagtcgtgc gccaccacgg tcttcaaccg gccggaccgc
    11521 ccggagtacg gcaccgtggg catgccgttg gccggcaccc gggtgcggat cgccgacgac
    11581 ggcgagatcc tgctgcgcgg cccgggcctg atggcgggct accacggcct ggactccgat
    11641 gcggtgctgg ccggcggctg gctgcacacc ggcgacatcg gcgagatcac cccgggcggg
    11701 tcgctgcgga tcaccgaccg caagaaggac ctgatcaaga cgtcgaacgg caagtacgtc
    11761 gcgccgcagt cgatcgaggc ccggttcaag gcgatctgcc cgctcgccgg ccagttcgtg
    11821 gtgcacggcg agaaccgcaa gtacatcacc gcgctgatcg acctcgaccc ggacgaggcg
    11881 cgccggtggg ccgccgcgca cggcctcgac ggtgtcacgc acgccgagat cgccggctcg
    11941 gccgacttgc gcgagaccct ccag
//
