LOCUS       NZ_CP023865            10225 bp    DNA     circular CON 27-APR-2024
DEFINITION  Actinoplanes teichomyceticus ATCC 31121 chromosome, complete genome.
ACCESSION   NZ_CP023865
VERSION     NZ_CP023865.1
KEYWORDS    RefSeq.
SOURCE      Actinoplanes teichomyceticus ATCC 31121
  ORGANISM  Actinoplanes teichomyceticus ATCC 31121
            Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
            Micromonosporaceae; Actinoplanes.
REFERENCE   1  (bases 1 to 8287152)
  AUTHORS   Yushchuk,O., Horbal,L., Fedorenko,V., Ostash,B., Luzhetskyy,A.,
            Kalinowski,J. and Ruckert,C.
  TITLE     Complete genome sequence of Actinoplanes teichomyceticus ATCC 31121,
            type strain of Actinoplanes teichomyceticus and producer of
            teicoplanin, isolated from soil near Nimodi, India
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8287152)
  AUTHORS   Yushchuk,O., Horbal,L., Fedorenko,V., Ostash,B., Luzhetskyy,A.,
            Kalinowski,J. and Ruckert,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2017) CeBiTec, Bielefeld University,
            Universitaetsstr. 27, Bielefeld, NRW 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8; canu v. 1.5
            Genome Coverage                   :: 184.1x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003711105.1-RS_2024_04_27
            Annotation Date                   :: 04/27/2024 03:18:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,294
            CDSs (total)                      :: 7,220
            Genes (coding)                    :: 7,116
            CDSs (with protein)               :: 7,116
            Genes (RNA)                       :: 74
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 27 of 104
            Pseudo Genes (incomplete)         :: 92 of 104
            Pseudo Genes (internal stop)      :: 11 of 104
            Pseudo Genes (multiple problems)  :: 22 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:55:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 335134
            Orig. end                         :: 345359
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP023865.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10225
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10225
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10225
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5225](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5225)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(89..102)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.75"
                     /tool="antismash"
     gene            340..1623
                     /locus_tag="ACTEI_RS01505"
     CDS             340..1623
                     /GO_function="GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /locus_tag="ACTEI_RS01505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="WP_122975990.1"
                     /transl_table=11
                     /translation="MPSPSAALTPTKRNLIVAAWIVAGIVAGLQAWGGLSRPLPDRLSD
                     MQVYLGAVGDLWGGGSLYDFAAQNGAAPFTYPPFAGLVFAVLHWVPFTPMAVIWEICTF
                     AVVVGIAVVVARRAEITFLDRRVLAPLLALVLFASAPISSNFRFGQISVFLVAAVLVDA
                     LQLASPRWRGIATGIAGAIKLTPMVFVPYYWFSGQRRTAIVSTITFVVCSGLGWLVLPG
                     DSVRFWFTEIWDVNRVGNIATGGNQSLNGAMLRWGLPEGARTAAAVIGLIVVAVALYRA
                     SLAYRQNRLLLAAVTVGAAGLVLSPVSWTHHQVWLVLGALLVVGASRALMIGWSVLVLL
                     VMIVPVTSLGSALGDEVVLGNARLWLAVALAAVVPAILRPAGNGTAPPAGRSGGSGHPS
                     AVQEGASTLPQRRTGAGPARTRDRGVLP"
     gene            1740..2690
                     /locus_tag="ACTEI_RS01510"
     CDS             1740..2690
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007071987.1"
                     /locus_tag="ACTEI_RS01510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphatase PAP2 family protein"
                     /protein_id="WP_239082544.1"
                     /transl_table=11
                     /translation="MGCWVALFVAAVLSWGLPTDPVYAFLWLWALAVAWRFGRPWREHL
                     AFARDWVPVVLLLTLYNLSRGFADNIRTPHVTEMIDADRWMFGWMTGGEVPTVWLQQRL
                     YVPDDVQWWEALVSMVYLSHFVASLGTAIVLWLVARHRWAQFMRRWFVLGLAGLATYFV
                     YPAAPPWWAAVNGYLDHSVPRLSLRGGQELGMHGAFSIVKLGQAAANPVAAMPSLHTAY
                     ALMVVLFFALMVRKRWWPLLALYPLAMTFTLVYSGEHYVIDVLVGWAYVVFAFTVVWLG
                     ERWWRERRARTAADGTPVRAEQREPSADGSVRTSV"
     gene            complement(3138..3371)
                     /locus_tag="ACTEI_RS01515"
     CDS             complement(3138..3371)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ACTEI_RS01515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_145830796.1"
                     /transl_table=11
                     /translation="MQTSSTAAKAYVTVASNSTYISGKLVIVTPSGSYSSCSGKYYSGD
                     TCSITKSHGSGTYRSAWNSVSGSTYEGGAVRV"
     misc_feature    3426..3437
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
     misc_feature    complement(3519..3534)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.42"
                     /tool="antismash"
     misc_feature    complement(3525..3540)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.36"
                     /tool="antismash"
     misc_feature    complement(3691..3704)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.61"
                     /tool="antismash"
     gene            complement(3822..4019)
                     /locus_tag="ACTEI_RS01520"
     CDS             complement(3822..4019)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477369.1"
                     /locus_tag="ACTEI_RS01520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_122975993.1"
                     /transl_table=11
                     /translation="MGRLYDTSEIADRLGVTRQRAHVITRQKGFPEPYDELVMGSVWRV
                     EDVEAWIREHRPEIAEDPES"
     misc_feature    complement(4089..4103)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.4"
                     /tool="antismash"
     gene            4427..4747
                     /locus_tag="ACTEI_RS01525"
     CDS             4427..4747
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ACTEI_RS01525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_122975994.1"
                     /transl_table=11
                     /translation="MERHEYPPAGVVHTADCEDAPPGLLTLPAQALRGSHDRPHRCVNS
                     AAVAANRPLVIERVAYEPHPYEESTIHPVDATKPLCRTCRGTHGVDPAAYRPQQPTLLL
                     PR"
     gene            complement(5001..5225)
                     /locus_tag="ACTEI_RS01530"
     CDS             complement(5001..5225)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /locus_tag="ACTEI_RS01530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YcaO-like family protein"
                     /protein_id="WP_122975995.1"
                     /sec_met_domain="YcaO (E-value: 3.4e-07, bitscore: 29.0,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAETIGRRHGMEMMVRDQTRPDLPLPTVKVLVPGLRPVAARFGP
                     GRLYDAPVAQGRLVTATRYEDVNPIPLPL"
     misc_feature    complement(5148..5150)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(5303..5941)
                     /locus_tag="ACTEI_RS01535"
     CDS             complement(5303..5941)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1215: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015623528.1"
                     /locus_tag="ACTEI_RS01535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_164465808.1"
                     /transl_table=11
                     /translation="MSDVTGLRELKKQATRERLAHQATVLFIEHGFDNVTIADVAAAAG
                     VSKMTVTNYFPLKEDLVFDAYALVEDSLAAAVRARRPGESVLHGLHRQYLAALGGTYPV
                     LTGHSSAGFARLVHESPRLRAREREIDERREEALAAALAAEDGAGDADHTRAAAAQLAA
                     AHRVLFRLVRSLVRDGMPQDQTDRRAAAAAAVSFGLLEPALGDYLVQTA"
     misc_feature    5973..5986
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.94"
                     /tool="antismash"
     misc_feature    6015..6030
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.68"
                     /tool="antismash"
     gene            6019..7452
                     /locus_tag="ACTEI_RS01540"
     CDS             6019..7452
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015623527.1"
                     /locus_tag="ACTEI_RS01540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MDR family MFS transporter"
                     /protein_id="WP_122975996.1"
                     /transl_table=11
                     /translation="MSDRLDPALLRVAAVLVPGAVLAQLDTTIVSVGIGSVAEGVHADL
                     LTVQWVTTGYLLAVALVAPLSGWLVHRFGGKRVWLLAVAVFVLGSALSGLATTAGALIA
                     FRALQGLGGGLMQPVGQALIARIAGPARIGRLVGLITTPVSLAPIAGPVLGGLLTTGPG
                     WRWMFFVNLPIGLAALVLAARLVPPDDAERDRTLRLDAPGLLLLPPGLVALVYGLSLSG
                     PDADPAARAALLGYGAIALIAYVAHALRTTRTPLLDLSLFAGRGFTLAAVNTFLIGAAL
                     YGSMLLIPLYLVQAYALSPLAAGLALAPQALGMAVVAPLAGRWTDRHGPRAVSLLGIAA
                     IVAGTVPFLLTGSRPPLFLLVGALFVRGLGLGAVVPPNAAATYTSVTRAQVPAATGVRT
                     VLNRIGGSIGTAVLAIILQTALGDSTADAAFGHTFGWVVAFAVLTLIPAAMYPRHAPGR
                     PAAGPAPGKTGTLVEGGTR"
     gene            7449..8333
                     /gene="mca"
                     /locus_tag="ACTEI_RS01545"
     CDS             7449..8333
                     /GO_process="GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mca"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PIG-L"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015623526.1"
                     /locus_tag="ACTEI_RS01545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="WP_122975997.1"
                     /sec_met_domain="PIG-L (E-value: 1.3e-20, bitscore: 73.1,
                     seeds: 137, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPPTRTDLSGLRLLSVHAHPDDESSKGAATLVKYSRAGADVLVC
                     TMTGGERGSVLNPALDRPEVRAELPARRRAEMARAREILGVRQRFLGFVDSGLVANGDV
                     PPDSFAAQPVDVATRPLVEAVREFRPHVMIAYDENGGYPHPDHIKANQVAVAAFLAAGD
                     PSRYQDCGPVWSPQKLYYTCAFNRDYFEAIHRAMLDAGLDSPAGEVLAGWPEDWPAWEV
                     TTRIECAEYFPIRRAALLAHETQVNPDGPELSCPLDLEMKVWPTEDYHLVSSSVPTHLP
                     ETDLFAGVVPDRP"
     gene            complement(8753..9943)
                     /locus_tag="ACTEI_RS01555"
     CDS             complement(8753..9943)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014444520.1"
                     /locus_tag="ACTEI_RS01555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_239082539.1"
                     /transl_table=11
                     /translation="MTTTAVPDLRATTRAVYLAFIFLGVAMASFAARIPQIRDELDLTP
                     SRLGLVLLAAAIGSITSLPLAGHIVGRFGSRHTVAVMAVVLGLSLSAVAVGYRFGVVPV
                     VTGLYVYGFATGAWDVAMNVQGAAVEQRLGRAIMPRFHAGYSIGTVAGALLGAGAVAAG
                     ISVTWHMVIMIVASAAIVTVAVQKFGPDQAAQDDSPAGERPSALTFWREPRTLLIGVFV
                     LAFAFTEGAGIDWISVAMIDDYGTSAGTGTLAFAVFLAAMTIGRWYGPGLLDRYGRVMV
                     LRALAVIGLAGLLLFAFGSHPVVAFAGALLWGIGASLGFPVGMSAAADDPAKAAPRVGV
                     VASIGYIAFLGGPPLIGFLGDHMTVSRGLLAVAALLVVSITLSSALKPLPPAPLER"
ORIGIN
        1 ttttgtttgg tctcgtaatg cttaggtcgt cggttcgatt ccgacaggcg gctcagaaag
       61 aaaccccagg tcaccgacct ggggtttctt cttttgatga attccacgac ggtccggtcc
      121 gtcgatggtt cgcatcctgc cgtgcgaccc ggtcgagcgg tccgccaccc ggtcgagggg
      181 cggctccttg atccaagggc ggcgcgggtg atccacaggt caggggcccg gtgggccagg
      241 cccggtcgat ccggtggcgc cggccgcccg gctcagggga gggcgacccg acatggcgcc
      301 ccgtatactt tgccctccac aaatcggaca ggatagttga tgccctcacc ctcggctgca
      361 ctcacaccta ccaagcgcaa tctgatcgtg gccgcctgga tcgtggccgg catcgtggcg
      421 ggattgcagg cttggggcgg gctgagccgg ccgttgccgg accggctctc cgacatgcag
      481 gtctacctcg gcgcggtggg cgatctgtgg ggcggcggca gcctctacga cttcgcggcg
      541 cagaacggcg cggcgccgtt cacctatccg ccgttcgccg gcctggtgtt cgctgtgctg
      601 cactgggtgc cgttcacgcc gatggccgtc atctgggaga tctgcacgtt cgccgtcgtg
      661 gtcggcatcg cggtggtggt ggcccggcgg gccgagatca ccttcctgga ccggcgtgtg
      721 ctcgccccgc tcctggccct cgtgctgttc gcgtccgcgc cgatctctag caatttccgg
      781 ttcgggcaga tcagcgtctt cctggtggcc gcggtcctgg tggacgccct ccagctcgcg
      841 tcgccgcgct ggcgggggat agcgaccggc atcgccgggg cgatcaagct gactccgatg
      901 gtcttcgtgc cctactactg gttctccggt cagcgtcgta ccgcgatcgt ctccaccatc
      961 acgttcgtgg tgtgcagcgg actcggctgg ctcgttctgc cgggcgactc ggtgcggttc
     1021 tggttcaccg agatctggga cgtcaaccgg gtgggcaaca tcgccaccgg cggcaaccag
     1081 tcgctcaacg gcgcgatgct gcgctggggt ctgcccgagg gcgcccggac cgccgccgcc
     1141 gtcatcggcc tgatcgtcgt tgccgtggcg ctgtaccggg cttcgctcgc gtaccggcag
     1201 aatcgcctgc tgctcgcggc cgtgaccgtc ggtgccgctg ggttggtgct ctccccggtg
     1261 tcctggacac atcaccaggt gtggttggtg ctcggcgcgc tgctggtggt cggcgccagc
     1321 cgggcgctca tgatcggctg gtcggttctg gtcctgctgg tgatgatcgt gccggtgacg
     1381 tccctggggt ccgcgctggg cgacgaggtc gtgctcggca acgcccggct ctggctggcc
     1441 gtggccctgg cggctgtcgt cccggccatt ctccggccgg ccgggaacgg gacggcgcca
     1501 ccggcgggcc ggagcggcgg atcgggccac ccatcagccg tccaggaagg cgctagtacc
     1561 cttccgcaac gcaggactgg ggccggtccg gctcgcaccc gtgatcgagg agttctccca
     1621 tgaccgacca tgtcgtcgcc gatgccgccg aaccaccggc gcgggtcgac caggagcacg
     1681 agcgaaccgc gcctgagctg gaccggaagg ccccggcggg ccggcgcagg acgctcgcga
     1741 tggggtgctg ggtggcgctg ttcgtcgccg cggtgctgtc gtggggtctg ccgacggacc
     1801 cggtctacgc gttcctgtgg ctgtgggcgc tcgctgtcgc ctggcgtttc ggccgtccct
     1861 ggcgcgagca cctggccttc gccagggact gggttcccgt cgtcctgctg ctgaccctct
     1921 acaacctgtc ccgcggcttc gccgacaaca tccgtacgcc gcacgtgacc gagatgatcg
     1981 acgccgacag gtggatgttc ggttggatga ccggcggcga ggtgccgacc gtctggctcc
     2041 agcagcgcct ctacgtaccg gacgacgtgc agtggtggga agcgctcgtc agcatggtct
     2101 acctctcgca cttcgtcgcc tcgctcggca ccgcgatcgt tctctggctg gtcgcccgtc
     2161 accgctgggc ccagttcatg cgtcgctggt tcgtcctcgg cctggccggc ctggccacgt
     2221 acttcgtcta cccggcggca cccccctggt gggccgcggt caacggctac ctggaccaca
     2281 gcgtgcctcg gttgagcctc cgcggcggcc aggaactggg tatgcacggc gccttcagca
     2341 tcgtcaagct gggccaggcg gccgccaacc cggtagccgc catgccgtcg ctgcacaccg
     2401 cctacgcgtt gatggtcgtg ctcttcttcg cgctgatggt ccgcaagcgt tggtggccgc
     2461 tgctggccct ctacccgctg gcgatgacat tcacgctggt ctactccggg gagcactacg
     2521 tcatcgacgt gctggtcggc tgggcctacg tggtcttcgc cttcaccgtc gtgtggctcg
     2581 gcgaacgctg gtggcgcgag cgccgcgcgc gaaccgcggc cgacggcaca cctgtacgcg
     2641 ccgagcagcg ggaacccagc gccgacgggt cggtgaggac ctcggtgtaa ccgcatcgac
     2701 cgagtcgctt gcggctcgcg gacagttcgc cttcgaagtg aattcgaagg cgtttctgtt
     2761 tgttgggaaa tcgtccgcga gcggtgcggc ccgattcgat tcgaccgcgt tcttcgggcg
     2821 gaccgtcttg tggatgtcgg cgtcggaggc cgggggtggt gcgaatccgg acgctgattc
     2881 gtgaatgcgt aacggaatgg gttacgcgat gaacgggtcc ggtgcgatcc tgtttctttc
     2941 cggacttatc cgtatggtcg gcgggatgga tggtccgccc tgactgacgc ggaactccga
     3001 gtgacgtatc tcgtgcgagg tcgtcactcg gagttccgcg aattcatgcc tggccggggt
     3061 gggatacggc atttcccgta cgtccgtcac cgtcctggac ggtcaccggg cgtacgccgc
     3121 gtctcccccg tgacgggtca gacccggacc gcgccaccct cgtaggtgga gccgctcacg
     3181 ctgttccacg cgctccggta ggtgccgctg ccgtgcgact tggtgatgga gcaggtgtcg
     3241 ccggagtagt acttgccgct gcagctgctg taggagccgc tgggcgtgac gatgaccagc
     3301 ttgccgctga tgtaggtgct gttgctggcc accgtgacgt aagccttggc ggcggtcgag
     3361 ctggtctgca ccacgcaaac cttgaaagag ccctggctca gggagtaggt gaggcagctg
     3421 ggggtgtatg cgaacgccgg cgaagccggg agaacggcga gcgatgcgac cgcggccgcg
     3481 cccgatgcga tagccgtcag gacgccacgc ttcaacttgc tattcatgct gattttcact
     3541 gagcctttcc ccgttgtgga acacgcgttg aacgccgagg agacgaaccg gaattcctcg
     3601 gtgcgcccga actctatatc aacgtggggg aacctttcaa tggatcttct ttatgggcgc
     3661 cgtacggtag atgggccccg gtcccgtggg tgacggtgag tgacgcgggt gggggcgcga
     3721 cggcgctcgg cgtggccggc ggcgccgagc gtggtgacgg tcccgcgccg gagagccgtg
     3781 ccagggtgct caccccgccg ggcggtcgcc cggctgtcgg ctcaggactc cgggtcctcg
     3841 gcgatctcgg gccggtgctc gcggatccac gcctcgacgt cctccacccg ccacaccgag
     3901 cccatcacga gctcgtcgta cggctccggg aagcccttct gccgggtgat gacgtgggcg
     3961 cgctggcgtg tgacgccgag ccggtcggcg atctcgctgg tgtcgtagag gcgtcccata
     4021 cccacatgat cacgctatgt actctcgcac ttgatggtga gaaacggtcg agtgcggcga
     4081 catctgtcaa cgacaggtgt aatgtccgcg acggcggcgg tgggcacgtc cagcgtcgag
     4141 agcggttgcg ctcgccgccg tccccaggtg acggcggggc cggttcccgt ctagacaaag
     4201 cgtggaaaat gcaccggcct cgccgtcgcc gaccccccgg agcgactcgg cgtggaggcg
     4261 atggcatgcg cgcacgacga accgtccgct ggtggatccg cccccggcga agcccgcgga
     4321 accactgccg gtgcggcatc cagagctgcc cgtacgcccc gtaccccgat gatgtcgagc
     4381 ggaccggagg cgacaccgtg atcaccacgc tgagggaggc agactgatgg agcgacacga
     4441 gtacccgccg gcgggcgtgg tgcacaccgc cgactgcgag gacgccccac ccggactgct
     4501 gaccctgccg gcgcaggcgc tcagggggtc gcacgaccgg ccgcaccgct gcgtcaactc
     4561 cgcggccgtc gcggcgaacc ggccgctggt gatcgagcgc gtcgcctacg agccccaccc
     4621 gtacgaggag tccaccatcc acccggtcga cgccaccaag cccctgtgcc ggacctgccg
     4681 gggcacccac ggcgtggatc cggcggcgta ccggccgcag cagccgaccc tgctgctgcc
     4741 ccgatgagcg ggcccgcgac gccggcccgc cacctcgcgg tgagctgcga caggtgcggg
     4801 gccgcatcgg gtgagccctg catcaactgg cgcaccggca aaccgctgac ggcgttctcc
     4861 gcgcacgaga cccggcgccg gaaggccgct cgatgaccgg cccggacggc gccggctgcc
     4921 gcggcatcgt ggcggggtcc ccgggaaggt tgatcgcgcg ccgctgtcac cgccaccgag
     4981 tcgccgatcc cggagggtgg tcagagcggc agcggtatcg ggttcacatc ctcgtaccgg
     5041 gtggcggtga cgagccgccc ctgagccacc ggggcgtcat agagtcgccc gggcccgaac
     5101 ctggcggcca ccggccgcag acccggaacc agcaccttga ccgtgggtaa tgggaggtcg
     5161 gggcgggtct ggtcgcggac catcatctcc atgccgtggc ggcggccgat cgtctcggcg
     5221 gccatgacgt cgtcgcgcag gcccttcccg ccgaggtggt ccggcccggg ctcaccgcgt
     5281 tcgccgctcc ggtccgcgac ggtcaggcgg tctggacgag atagtcgccc agggccggct
     5341 cgagcagccc gaagctgacc gcggccgcgg cggcggcccg gcggtcggtc tggtcctgcg
     5401 gcatgccgtc gcgcaccagg gagcggacca gccggaacag cacccggtgt gcggcggcga
     5461 gctgggccgc ggcggcacgg gtgtggtcgg cgtccccggc cccgtcctcg gcggcgagcg
     5521 cggcggcgag tgcctcctcc cgccgctcgt cgatctcgcg ttcccgggcg cgcaaccgtg
     5581 ggctctcgtg caccaggcgg gcgaagcccg cggaggagtg cccggtgagc accgggtacg
     5641 tcccgcccag cgcggccagg tactgccggt gcagcccgtg cagcaccgac tcgccggggc
     5701 ggcgggcgcg caccgcggcg gccaggctgt cctcgacgag ggcgtacgcg tcgaagacga
     5761 gatcctcctt gagcgggaag tagttggtga ccgtcatctt cgacacgccg gccgccgcgg
     5821 ccacatcagc gatggtgacg ttgtcgaacc cgtgctcgat gaacagcacg gtggcctggt
     5881 gggcgagccg ttccctggtt gcctgcttct tcagttcgcg cagccccgtc acgtcgctca
     5941 tgccggtcat ggtagccgca ttgtgctcaa cataaaatta tgcttgacat aaatatttac
     6001 tcgacctaag ttcgtcgcat gtccgatcgc ctcgaccccg cactgctccg cgtggccgcc
     6061 gtgctcgtcc cgggcgccgt gctggcccaa ctggacacca cgatcgtcag cgtcggcatc
     6121 ggctccgtgg ccgagggcgt gcacgccgac ctgctcaccg tgcagtgggt gaccaccggc
     6181 tacctgctcg ccgtcgcgct cgtggcgccg ctctccggct ggttggtcca ccgcttcggc
     6241 ggcaagcgcg tctggctgct ggccgtcgcg gtgttcgtcc tcggctcggc gctcagcggg
     6301 ctcgccacga cggccggcgc cctgatcgcg ttccgggcgc tgcagggcct cggcggcggg
     6361 ctcatgcagc cggtcgggca ggcgctgatc gcccggatcg ccggaccggc tcgcatcggc
     6421 cggctcgtcg ggctcatcac cacgccggtc tcgctcgcgc cgatcgccgg accggtcctg
     6481 ggcggcctgc tcaccaccgg ccccggctgg cgctggatgt tcttcgtcaa cctgccgatc
     6541 ggcctggccg cgctcgtgct ggccgcccgg ctggtcccgc ccgacgacgc cgagcgggat
     6601 cgcacgctgc gcctcgacgc gcccggtctg ctgctgctcc cacccggcct ggtcgccctg
     6661 gtctacggac tctcactgag tggcccggac gccgacccgg cggcccgcgc cgccctgctg
     6721 gggtacgggg ccatcgcgct gatcgcctac gtggcgcacg ctctgcggac cacccggacg
     6781 cccctgctcg acctgagcct gttcgccggc cgcggcttca ccctcgccgc ggtcaacacc
     6841 ttcctgatcg gcgccgcgct gtacggatcg atgctgctga tccccctgta cctcgtgcag
     6901 gcgtacgcgt tgagcccgct cgccgccggc ctcgccctgg ccccgcaggc actgggcatg
     6961 gccgtggtgg ccccgttggc gggtcgctgg accgaccggc acggcccccg cgcggtgtcc
     7021 ctgctcggca tcgcggccat cgtcgccggc accgtcccgt tcctgctcac cggctcccga
     7081 ccgccactgt tcctgctggt gggggcgctg ttcgtccggg gcttggggct cggcgcggtg
     7141 gtgccgccga acgcggccgc cacgtacacg tcggtcaccc gcgcgcaggt tccggccgcc
     7201 accggggtgc gtaccgtgct gaaccgcatc ggcggctcga tcggtacggc cgtgctggcc
     7261 atcatcctgc agacggcgct gggcgactcc accgcggatg ccgcgttcgg ccacaccttc
     7321 ggctgggtgg tcgcgttcgc cgtactgacc ctgatccccg ccgcgatgta tccgcggcac
     7381 gcccccggca ggcccgcggc cggtcccgcg cccgggaaga ccgggacgct cgtcgaagga
     7441 ggtacccgat gaccccgccc acccgcaccg acctgtccgg gctgcgactg ctcagcgtgc
     7501 acgcccatcc ggacgacgag tccagcaagg gcgccgccac gctggtgaag tactcccggg
     7561 ccggcgcgga cgtcctggtc tgcaccatga ccggcggtga gcgcggcagc gtgctcaacc
     7621 cggcgctgga ccgcccggag gtccgggccg agctgcccgc ccggcgccgg gccgagatgg
     7681 cccgcgcccg cgagatcctc ggggtgcggc agcggttcct cggcttcgtc gactccggcc
     7741 tggtcgccaa cggtgacgtg ccgccggaca gcttcgcggc gcaacccgtc gacgtcgcga
     7801 cccggccgct ggtcgaggcg gtccgcgagt tccggccgca cgtcatgatc gcgtacgacg
     7861 agaacggcgg gtacccgcac ccggatcaca tcaaggccaa ccaggtcgcg gtggccgcct
     7921 tcctggccgc cggcgacccg tcgcgatatc aggactgcgg tccggtgtgg agcccgcaga
     7981 agctctacta cacctgcgcc ttcaaccggg actacttcga ggccatccac cgggcgatgc
     8041 tcgacgccgg cctggactcc ccggccggtg aggtgctggc cggctggccc gaggactggc
     8101 cggcctggga ggtgaccacc cgcatcgagt gcgccgagta cttcccgatc cggcgggccg
     8161 cgctgctggc ccacgagacg caggtgaacc cggacggccc ggaactgtcc tgcccgctgg
     8221 acctggagat gaaggtctgg cccaccgagg actaccacct cgtctcctcg tcggtgccga
     8281 cccacctgcc ggagaccgac ctgttcgccg gggtggtgcc ggaccggccc tgaacgcgtc
     8341 caccgctgtt cgaggccggc gcgagccggc ccggacacgc tggcccggtc cgacatcacc
     8401 ggcatcggac agaggccagc atgcgcgcgg tgggcgcagc cgtaaccggc ggtgaaaccc
     8461 cggcagcccg ggccgtggca gccgcggtgg acgctttgcc gatcgctggc ccgcggctcg
     8521 aagcggaccg ctctcaccct gaccccgccg gccgtggcgg cgttcctcac cgcctccgcg
     8581 ctcgccccgg tcgccgccgc gctcgtgctc cccccggccg cggccgtcgc cgcggtcgtc
     8641 gcccagctcg gcggcatggg caccaactat ctcgcggacg tggtcgccga ggcgggccgc
     8701 cgggtccgtg tcccggacgg cggccgccga cgggctggcc cccgcccacc gcctaccgtt
     8761 cgagcggggc gggcggcagc ggcttgagcg ccgaggacag cgtgatcgag acgaccagca
     8821 gcgcggcgac ggcgagcagg ccgcgggaca cggtcatgtg gtcgccgagg aagccgatca
     8881 gtggcgggcc gccgagaaag gcgatgtagc cgatcgaggc caccacgccg acccggggcg
     8941 cggccttggc cgggtcgtcg gcggccgcgc tcatcccgac cgggaagccg agtgacgccc
     9001 cgatgcccca cagcagcgcg ccggcgaagg cgaccaccgg atgtgagccg aaggcgaaca
     9061 gcagcaggcc ggccaggccg atcacggcga gcgcgcgcag caccatgacc cggccgtacc
     9121 ggtccagcag gccggggccg taccagcggc cgatggtcat ggcggccagg aacaccgcga
     9181 aggcgagcgt gccggtgccg gccgaggtgc cgtagtcgtc gatcatggcg acgctgatcc
     9241 agtcgatgcc ggcgccctcg gtgaacgcga aggcgagcac gaacaccccg atcagcaggg
     9301 tgcgcggctc ccgccagaag gtgagggcgg acgggcgttc gccggccggc gagtcgtcct
     9361 gggcggcctg gtccggcccg aacttctgga ccgccaccgt gacgatcgcg gccgatgcca
     9421 cgatcatgat caccatgtgc caggtgaccg agatgccggc ggccaccgcg cccgctccca
     9481 ggagcgcgcc ggccaccgtg ccgatgctgt agccggcatg gaaccgcggc atgatcgccc
     9541 ggccgagccg ctgctccacc gccgcgccct gcacgttcat cgccacgtcc cacgcgccgg
     9601 tggcgaagcc gtacacgtag aggccggtga ccaccgggac caccccgaac cggtagccga
     9661 ccgccaccgc gctcagcgac agcccgagca ccacggccat cacggccacc gtgtgccgcg
     9721 agccgaaccg gccgacgatg tgcccggcca gcggcagcga ggtgatcgac ccgatcgcgg
     9781 cggccagcag caccagcccc agccgggacg gggtcaggtc cagctcgtcg cggatctgcg
     9841 gaatccgggc ggcaaagctg gccatcgcga cgcccaggaa gatgaaagcg aggtagaccg
     9901 cacgggtggt cgcccgcagg tccggcacgg cggtagtggt caccgcgtga cggtagtcgc
     9961 tcgcgctgcg ggccgcaccg ccgcgcatgt ctagactcgg atccgtgctg gtggagccgg
    10021 tctgggacgt cgcagtgatc ggagcgggcc cggccgggct ggcggccgcg cacgccgccg
    10081 cgtcccaggg ggcgcgggtc gtggtgctgg agcgcgccgc gcacccgcgg tacaagacct
    10141 gcggcggggg cctgctgggc agttcgctgc ggttgagctc ggagcggctc gcggttccgg
    10201 tgcgggacac catcaccgac atcac
//
