LOCUS       NZ_CP033073            11332 bp    DNA     linear   CON 23-OCT-2024
DEFINITION  Streptomyces dangxiongensis strain Z022 chromosome, complete genome.
ACCESSION   NZ_CP033073
VERSION     NZ_CP033073.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces dangxiongensis
  ORGANISM  Streptomyces dangxiongensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8085191)
  AUTHORS   Zhang,B.
  TITLE     The genome of Streptomyces dangxiongensis Z022
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8085191)
  AUTHORS   Zhang,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2018) State Key Laboratory of Cryospheric
            Sciences, Chinese Academy of Sciences, Donggang West Road 320,
            Lanzhou, Gansu 730000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. version 0.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 213.0x
            Sequencing Technology             :: Illumina and Pacpio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003675325.1-RS_2024_10_23
            Annotation Date                   :: 10/23/2024 04:16:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,241
            CDSs (total)                      :: 7,151
            Genes (coding)                    :: 6,614
            CDSs (with protein)               :: 6,614
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 537
            CDSs (without protein)            :: 537
            Pseudo Genes (ambiguous residues) :: 0 of 537
            Pseudo Genes (frameshifted)       :: 320 of 537
            Pseudo Genes (incomplete)         :: 281 of 537
            Pseudo Genes (internal stop)      :: 26 of 537
            Pseudo Genes (multiple problems)  :: 83 of 537
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:47:29
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1744544
            Orig. end                         :: 1755876
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP033073.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Binglin Zhang.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11332
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="13"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11332
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11332
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6332](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6332
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(65..80)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 16.81"
                     /tool="antismash"
     gene            108..1673
                     /locus_tag="D9753_RS07745"
                     /old_locus_tag="D9753_07745"
     CDS             108..1673
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675665.1"
                     /locus_tag="D9753_RS07745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="D9753_07745"
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_121786330.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 7.1e-07, bitscore:
                     27.4, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="DAO (E-value: 8.7e-47, bitscore: 159.1,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHEQRRVTDSYWLRTAPGPDHPTLTGDGDVDVAVVGAGIAGLSTA
                     HELARAGLRVAVLEAGRAAAGVTGRTTAKLTAQHTLVYDRLRRTRGPGGARLYARSQTE
                     AIEHAAALVAELGIECEWETRDAYTYVRDPSRADEVRAEAEAAREAGLPAAFTTETGLP
                     FPVAGAVRVGGQAQFHPVKYLRAITADLIAHGGQVYEHTRVTGLTEGEPCRLTTEGGAI
                     VTARDVVVATHYPVFDRALLFSRLSPRRELVVAGPLGPGPDPDGMYITPDENTRSVRTA
                     PYGPDGQRMLVVTGEHFTPGTGDPEAGFARLAAWAEEHFPGVSLDHAWATQDNESTDTV
                     PLVGPFHPGARHTYVATGFGGWGLSGGIMAGLLLTALITGRDCAWRGLYDPRRLKSVVR
                     EAPSLLKTQAEVARHFVGDRLKPPASVDELAPGDGALVRSGGERVAVHRDDEGTLHAVS
                     ARCTHMGCLVSFNRAERAWECPCHGSRFDVDGAVIEGPAVNPLERRDLDGSPTDAHRPA
                     PPRG"
     gene            1737..2585
                     /locus_tag="D9753_RS07750"
                     /old_locus_tag="D9753_07750"
     CDS             1737..2585
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523442.1"
                     /locus_tag="D9753_RS07750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="D9753_07750"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_121786331.1"
                     /transl_table=11
                     /translation="MIPHLRRITAVCAVLAAALTACGTTDGERPAPAVSPVSASPSSTR
                     PPVLAPGPGGLTPVFEHGPRTRDKTVAFTFDADMTADQGPRAASGEHFDNPGLISALRA
                     LKVPATVFMTGRWADQYPAEARSLGHDPRFEVANHSWSHYAFTADCYGLPTVDADGMRA
                     DVERAYASLRTAGVPHAMPYFRFPGGCYDQRALRALSGLGVTAVQWDVVSGDAFATDAD
                     AVTRQVLDGVKPGSVVVMHCTRSAAPTTEQVVRTVVPELRRRGYRFVKVSELIGRSGGH
                     R"
     gene            2604..2786
                     /locus_tag="D9753_RS07755"
                     /old_locus_tag="D9753_07755"
     CDS             2604..2786
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675663.1"
                     /locus_tag="D9753_RS07755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="D9753_07755"
                     /product="hypothetical protein"
                     /protein_id="WP_121786332.1"
                     /transl_table=11
                     /translation="MSDEDRCTIADATRPATAEGPPYAECVLCREPTEYPESYKGITLC
                     PVCEWQEAQRTACSG"
     gene            2921..3661
                     /locus_tag="D9753_RS07760"
                     /old_locus_tag="D9753_07760"
     CDS             2921..3661
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019981772.1"
                     /locus_tag="D9753_RS07760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="D9753_07760"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_121786333.1"
                     /transl_table=11
                     /translation="MSHVSAPSADSPSDSPREPRDLAPQFVLPLVVRIERDAPPARTDA
                     LETAARAVLVLLGDDRARGDGEWAEAVRNWEDARIRKVVRRARGAEWRRAGTLPGITVT
                     GRSAEVRVFPPIPLDGWPKDLAKLQVSGTELDDPEPPVAADPAQPVLWLNPELEMSAGK
                     AMAQTGHGAQLAWWALSDADRTAWRDAGFPLSVRTAARADWPRLTTSGLPLVRDAGFTE
                     IAPGLTVAVEGVDRVSSLPRRQQP"
     gene            complement(4064..4219)
                     /locus_tag="D9753_RS37755"
     CDS             complement(4064..4219)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="D9753_RS37755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_240468076.1"
                     /transl_table=11
                     /translation="MLHTEEYATWCMERFGYFLHHRPTSRTRTNGLMANTVDKIRAQGF
                     EVDDRL"
     gene            complement(<4522..4818)
                     /locus_tag="D9753_RS07770"
                     /old_locus_tag="D9753_07770"
                     /pseudo=""
     CDS             complement(<4522..4818)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019984255.1"
                     /locus_tag="D9753_RS07770"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="D9753_07770"
                     /product="HAD family hydrolase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MIDTIVFDVGETLTRDDRYWASWADWLGVPRHTLSALVGAVVAEG
                     RDNADALRLLRPDIDVAAEYEAREAAGRGEHLDETDLYDDVRPALAALRGAQAR"
     misc_feature    4787..4802
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.37"
                     /tool="antismash"
     gene            5001..6332
                     /locus_tag="D9753_RS07775"
                     /old_locus_tag="D9753_07775"
     CDS             5001..6332
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125240.1"
                     /locus_tag="D9753_RS07775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="D9753_07775"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_121786334.1"
                     /sec_met_domain="DUF692 (E-value: 3.2e-95, bitscore: 317.1,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGRLGTGIGWRPEIAEAVEGMPGIDWVEVVAENVCPGHLPESLLR
                     LRERGVTVIPHGVSLGLGGADRPDEGRLAALAERAEALGAPLVTEHIAFVRAGGALTAS
                     PPLEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLALENIAALFSWPGEELTEGQFLS
                     ELVERTGVRLLIDVANLHTNRVNRGEDPAEALAALPLEAIAYVHVAGGFERDGVWHDSH
                     AHPVPRPVLDILTGLATRVAPPGVLLERDDDFPGPAELERELHTIRDAVAAGRSRSAGG
                     TTPTGTATGGAVGTGAGGVGVLAPPVAGVRERLGVAQTAVLSSLVAGTPVPEGFDRARM
                     RVQARALAAKRADVVAKVAPELPVILGAEYREAFLAYARQRPMTAGYRRDALDFAGHLL
                     KLGRPGNAGTRRRLRQWWLDRAGPAPRRPSPTTRALRALLRRRA"
     misc_feature    complement(6610..6626)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.41"
                     /tool="antismash"
     gene            6677..7447
                     /locus_tag="D9753_RS07780"
                     /old_locus_tag="D9753_07780"
     CDS             6677..7447
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675657.1"
                     /locus_tag="D9753_RS07780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="D9753_07780"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_121786335.1"
                     /transl_table=11
                     /translation="MRPRPPIEGRGIFSGTGLVVAALAATVVALLVPLWSYAGRQDPAS
                     ANVLSAATVTTPYGPLSARDRDFVTKVRLAGLWELPAGELAQRKGTTPAVRTAGRHLVD
                     GHTSLDAHVRTVATQLGVALPNEPNAQQKQWLATLDAARGQDFDRQFAGLLRLAHGRVF
                     SLVAEVRAGTQNSLVRDLADDANTTVLDHIKILEATGYVDFSALARDLASPASPVPTPP
                     EADPGAAVPVTPSPGETYALPPATAGPPPAASGP"
     gene            7574..8353
                     /locus_tag="D9753_RS07785"
                     /old_locus_tag="D9753_07785"
     CDS             7574..8353
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675656.1"
                     /locus_tag="D9753_RS07785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="D9753_07785"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_121786336.1"
                     /transl_table=11
                     /translation="MFWVLLLLLAWAFAGTACALLCLAALRAATAEARDASTDRERGLS
                     LYEAAFLSGGPARVADVTLVSMARQRRLLLAHTGWATVVDPRGRDEMERSVIGAIGPEG
                     QSRIAPVRAQAAGAEAVRRLADGLERAGLAVPEGTGVTVGAAVRRVRAAAVAVPALGAT
                     ALLLPVQTGTPRLLVGLWFALPLTLSLSCLAIARFEVHPYSRWASPAGQRLLGALAGRR
                     DGDERSFLTSVAVRGIRAIGEPELRAAFTHRDQPWRE"
     gene            8524..10194
                     /locus_tag="D9753_RS07790"
                     /old_locus_tag="D9753_07790"
     CDS             8524..10194
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (117-478). E-value:
                     1.4e-18. Score: 59.3. Matches aSDomain:
                     nrpspksdomains_D9753_RS07790_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675655.1"
                     /locus_tag="D9753_RS07790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="D9753_07790"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_121786337.1"
                     /sec_met_domain="PF00561 (E-value: 1.3e-21, bitscore: 76.0,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKAAALYSAAGSLLLTSLAAAPAGGAPGSPGAFGAPGAAELRGTA
                     VAVARARAAGIAFGPCAAADVPQAPAELRCGTVTVPLDYAHPDGRKIELTVSRTRATQK
                     DPHNSKRKVPRQGALVYNPGGPGASSMYFPLIGKIPQWKRTAAAYDLVGYAPRGVGRSA
                     PLSCEDPKHFFKAPTASPAFPSQAFKRQRVAQAKAYARGCAQRSGSGLRFYTSLANARD
                     LDVLRAALGEDRLTFMGASYGTYFGALYATMFPSHVRRMVLDSAVNPDPEKVWYRNNLD
                     QSAAFEERWADFREWIARHDDVYGLGDTPAKVQRAYDTARERLAGKAAGGRVGPGQLQN
                     AFLSAGYYDDFWPSRAEALAAFLRGDPEPLVRLAAPSPDTAVEAENGRAVYTAVECNDA
                     PWPTRWKVWDRDNTRLARVAPFETWDNAWMNLPCAYWPAPRQRPLDVRTAPGELPPVLI
                     LAAQRDAAAPYQGALETNRRLAGSVLVTERDAGTHGVGGGPNPCVNAYLDAYLLQGRLP
                     ARHTSCAPRPEPEPDTPAAGAPAPGSTSRDAVKGRAAGR"
     aSDomain        8875..9957
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_D9753_RS07790_Abhydrolase_1.1"
                     /evalue="1.40E-18"
                     /label="D9753_RS07790_Abhydrolase_1.1"
                     /locus_tag="D9753_RS07790"
                     /protein_end="478"
                     /protein_start="117"
                     /score="59.3"
                     /tool="antismash"
                     /translation="ALVYNPGGPGASSMYFPLIGKIPQWKRTAAAYDLVGYAPRGVGRS
                     APLSCEDPKHFFKAPTASPAFPSQAFKRQRVAQAKAYARGCAQRSGSGLRFYTSLANAR
                     DLDVLRAALGEDRLTFMGASYGTYFGALYATMFPSHVRRMVLDSAVNPDPEKVWYRNNL
                     DQSAAFEERWADFREWIARHDDVYGLGDTPAKVQRAYDTARERLAGKAAGGRVGPGQLQ
                     NAFLSAGYYDDFWPSRAEALAAFLRGDPEPLVRLAAPSPDTAVEAENGRAVYTAVECND
                     APWPTRWKVWDRDNTRLARVAPFETWDNAWMNLPCAYWPAPRQRPLDVRTAPGELPPVL
                     ILAAQRDAAAPYQGALETNRR"
     gene            complement(10248..10979)
                     /gene="hemQ"
                     /locus_tag="D9753_RS07795"
                     /old_locus_tag="D9753_07795"
     CDS             complement(10248..10979)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="D9753_RS07795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="D9753_07795"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_121786338.1"
                     /transl_table=11
                     /translation="MSDDAPIIESGRVPNKGKLAKDLNEVIRYTLWSVFRLKDVLPEDR
                     TGYADEVQELFDQLAAKDVTVRGTYDVSGLRADADVMVWWHAETSEQLQEAYNLFRRTR
                     LGRALEPVWSNMALHRPAEFNRSHIPAFLADETPRDYVSVYPFVRSYDWYLLPDEDRRR
                     MLADHGKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARMHVR
                     EEVPFYTGRRKDVADLVAGLA"
ORIGIN
        1 gcgggagctg gatccgcggt atccggaggg cgagttcgac gtggcggagt gccgggagcg
       61 gctgctgaag cagacgtgag ccggacgggc cggggtaccc ggcgggcatg cacgagcagc
      121 gaagggtcac cgattcgtac tggctgcgga ccgcccccgg gccggaccac cccaccctga
      181 ccggtgacgg cgacgtcgac gtcgccgtcg tcggcgcggg gatcgccggg ctcagcaccg
      241 cccatgagct ggcccgggcc gggctgcggg tggccgtgct ggaggccggg cgggccgcgg
      301 ccggcgtcac cggccgcacc accgccaagc tgaccgccca gcacaccctg gtctacgacc
      361 ggctccggcg tacccgcggc cccgggggag cccggctgta cgcccgctcg cagaccgagg
      421 cgatcgagca cgcggcggcg ctggtggccg agttgggcat cgagtgcgag tgggagacca
      481 gggacgcgta cacctacgtc cgggacccgt cgcgggcgga cgaggtgcgc gcggaggcgg
      541 aggccgcccg ggaggcgggt ctgccggccg cgttcaccac ggagaccggg ctgccgttcc
      601 cggtggcggg cgcggtccgg gtgggcggac aggcacagtt ccacccggtc aagtacctgc
      661 gggcgatcac cgccgacctc atcgcgcacg gcggccaggt gtacgagcac acacgggtca
      721 ccggccttac ggagggcgag ccgtgccggc tgacgaccga gggcggcgcc atcgtgaccg
      781 cccgggacgt cgtggtcgcc acccactacc cggtgttcga ccgggccctg ctgttcagcc
      841 ggctgtcccc gcgccgggaa ctggtcgtcg ccggtccgct gggccccggc cccgacccgg
      901 acggcatgta catcacgccg gacgagaaca cccgctcggt gcgcacggca ccctacggcc
      961 cggacgggca gcggatgctc gtcgtcaccg gggagcactt cacgcccggc accggtgatc
     1021 cggaggctgg tttcgcacgg ctcgccgcct gggccgagga gcacttcccc ggggtgagcc
     1081 tggaccacgc ctgggccacg caggacaacg agtccaccga cacggtcccg ctggtcggcc
     1141 ccttccaccc gggcgcccgg cacacgtacg tcgccaccgg gttcggcggc tggggactga
     1201 gcggcggcat catggccggg ctgctgctga ccgccctgat caccggccgg gactgcgcct
     1261 ggcgggggct gtacgacccg cgccggctga agtcggtggt ccgcgaggcg ccgtcgctgc
     1321 tgaagacaca ggcggaggtg gcccggcact tcgtcggcga ccggctgaaa ccgccggcct
     1381 cggtggacga gctggccccc ggcgacggcg cgctggtccg gtccggcggc gaacgggtcg
     1441 ccgtccaccg cgacgacgag ggcaccctgc acgccgtgtc ggcgcgctgc acccacatgg
     1501 gctgcctggt ctccttcaac cgtgccgaac gcgcctggga gtgcccctgc cacgggtccc
     1561 gcttcgacgt ggacggcgcg gtgatcgagg gaccggcggt caacccgctg gagcggcgcg
     1621 acctcgacgg ctccccgacg gacgcgcacc gaccggcgcc gccgcgcggg tgaccgtacc
     1681 gccgcgccat gagtcatatc accgtacgaa cggacatcgc accctacgtt cgtacggtga
     1741 tcccacacct acgtcgtatc accgccgtct gcgccgtcct ggccgcggcg ctcaccgcct
     1801 gcggtaccac ggacggtgag cgccccgccc cggccgtctc ccccgtctcc gcgtccccct
     1861 cctccacccg tccgcccgtg ctcgcgccgg gccccggcgg gctgaccccg gtcttcgagc
     1921 acggcccccg cacccgggac aagacggtgg cgttcacctt cgatgccgac atgaccgccg
     1981 accaggggcc ccgcgcggcc tccggcgagc acttcgacaa cccgggcctg atcagcgccc
     2041 tgcgcgcgct gaaggtgccg gcgaccgtct tcatgaccgg ccggtgggcg gaccagtacc
     2101 cggccgaggc ccgcagcctc ggccatgacc cgcggttcga ggtcgccaac cactcctgga
     2161 gccactacgc gttcaccgcc gactgctacg gcctgccgac cgtcgacgcc gacgggatgc
     2221 gggcggacgt cgagcgggcg tacgcctcgc tgcgcacggc cggcgtgccg cacgcgatgc
     2281 cgtacttccg cttccccggc ggctgttacg accagcgggc cctgcgggcg ctgagcgggc
     2341 tcggggtgac cgcggtgcag tgggacgtgg tgagcggcga cgcgttcgcg acggacgccg
     2401 acgcggtgac ccggcaggtg ctcgacgggg tgaagccggg ctcggtggtc gtcatgcact
     2461 gcacccgcag cgccgccccg accaccgagc aggtcgtccg cacggtcgta ccggagctgc
     2521 gccggcgggg ctaccggttc gtgaaggtct ccgagctgat cggccggagc ggcggacacc
     2581 gctgaccgtc atacactgag ggtatgagcg acgaggaccg ctgcaccatc gccgacgcga
     2641 cgcggcccgc gacggccgaa ggcccgccgt acgcggagtg tgtgctgtgc cgcgaaccga
     2701 cggagtaccc ggagtcgtac aagggcatca ccctgtgccc cgtctgcgag tggcaggagg
     2761 cccagcgcac cgcctgctcc ggttgacggg gcgggggggg ccggcctccg tgccccggac
     2821 ggccgccgac cgggcgcggg ggccgggctc cgcgtccggt cggccgccga cgggcatgcg
     2881 cgttgatcgg tccgttcacg gcttcgtagg ctgggctgct gtgagccacg tatccgcccc
     2941 cagtgccgac agcccctccg actccccgcg cgagccccgc gacctggccc cccagttcgt
     3001 cctgccgctc gtcgtacgga tcgaacgaga cgcgccgccc gcccgcaccg acgcgctgga
     3061 gacggccgcc cgggccgtgc tcgtcctgct cggcgacgac cgggcccgcg gcgacggcga
     3121 gtgggccgag gccgtccgga actgggagga cgcccgcatc cggaaggtgg tccgccgggc
     3181 ccgcggcgcc gagtggcggc gcgccgggac cctccccggc atcacggtga ccggcaggtc
     3241 cgccgaggtc cgggtcttcc cgccgatccc gctggacggc tggcccaagg acctggcgaa
     3301 gctccaggtg tcgggcacgg aactggacga cccggagccg cccgtcgccg ccgacccggc
     3361 acagcccgtg ctgtggctga acccggagct ggagatgtcg gccggcaagg cgatggccca
     3421 gacgggccac ggcgcccagt tggcctggtg ggcgctgtcc gacgcggacc gcaccgcgtg
     3481 gcgggacgcc ggcttccccc tgtccgtccg cacggccgcc cgcgccgact ggccccgcct
     3541 gaccacgagc ggcctccccc tcgtccggga cgccggcttc acggagatcg cgccggggct
     3601 gactgttgcg gtggagggcg tcgatcgcgt cagctcactg cctcgccgac agcagccgtg
     3661 accctccctc tcgcgtcatg ttcccctcct tcccatgggt cgggacccgg cgtctcgacc
     3721 ggaacttcgc tgtgcctgtg ctcaccgacg aacagatcgc gccgaagccg accgcctatc
     3781 cccattacgc ggactgacgc cccgaccact ctcactctcg cgaaggccaa gcgaaggcga
     3841 gctgttggtc ggctacgagc ctgctcgcgt aggcgaagac gggcccgaac atgctgaaga
     3901 cggtcaccct gtcgcggccc ttggcagctt cagcggtcgc catgaaaact cccagtttgt
     3961 acttacaagc ccggatcgta acggctgcca ctgacagtgg ccggcatcag cagcagggac
     4021 cgtcgccgca gcaggtcggc gggttgcatt cggcagcggt tccttacagc cggtcgtcga
     4081 cctcgaaccc ctgcgcgcgg atcttgtcga ccgtgttggc catcaggccg ttggtccgcg
     4141 tcctgctggt gggccggtga tgcaggaagt agccgaaccg ctccatgcac caggtggcgt
     4201 actcctcggt gtggagcatg aaggtgtgcc agccgccgct cgaacagcgc gggcgacacg
     4261 aggtcccggc cgcggcgcag agcggccggc ctttcaggag ccactgtcat ttcgcagtct
     4321 ttccggcagg aacaggtcgc cggcccggtg ctccgggcac ggccgcagcc gcgggtacgc
     4381 ggcgggtgca catcggctgg gtgggctttg acgatgtgcc tggccacgaa gcagtccggc
     4441 gatcgccctc atgactgctc gcggaccggg acctcgccgt cgagcttctc gcagtggtca
     4501 cacagggcgt tgacgctgcg ggcgagcttg cgcgccacgc agggcggcga gggcggggcg
     4561 cacgtcgtcg tacaggtcgg tctcgtcgag gtgctcaccg cggcctgcgg cctcgcgcgc
     4621 ctcatactcg gcggcgacgt cgatgtccgg ccggaggagc cgcagcgcgt cagcgttgtc
     4681 acggccctcg gcaacgacgg caccaacaag cgcggacagg gtatggcgag ggacgccgag
     4741 ccagtccgcc cacgatgccc agtaccggtc gtcacgggtg agcgtctcgc cgacatcgaa
     4801 cacgatggtg tcaatcacgc agcggagcct acggtgcctc accgggatcc tcgcgggccg
     4861 ggcgtcggag cggcggcagg cccggggccg aacctcaaat gttgctctga acgcggcggg
     4921 gagggggttc gcggcccgtc ggcggggcca aacttccctc actcgacgtg cgaacgccgg
     4981 ccgaggaggg ggaacgcatc atggggcgac tggggaccgg gatcgggtgg cggccggaga
     5041 tcgcggaggc cgtggagggg atgccgggca tcgactgggt ggaggtcgtg gcggagaacg
     5101 tctgccccgg tcatctcccg gagtcgctgc tgcggctgcg cgagcgcgga gtgacggtga
     5161 tcccgcacgg cgtctccctc ggtctcggcg gcgcggaccg tcccgacgag gggcggctgg
     5221 cggcgctcgc cgagcgggcg gaggcgctgg gggcacccct ggtcacggag cacatcgcct
     5281 tcgtccgggc gggcggggcg ctcaccgcgt ccccgcccct ggaggccggg cacctgctgc
     5341 ccgtgccgcg cacccgggac gccctggacg tgctgtgcga gaacgtccgc atcgcccagg
     5401 acgcactgcc ggtgccgctg gcgctggaga acatcgccgc gctgttctcc tggccgggcg
     5461 aggaactgac cgaggggcag ttcctgtccg agctggtcga gcgaaccggc gtgcgcctgc
     5521 tcatcgatgt ggccaacctg cacaccaacc gcgtcaaccg gggcgaggac ccggccgagg
     5581 cactggccgc gctgccgctg gaggcgatcg cctacgtcca tgtcgcgggc ggcttcgagc
     5641 gcgacggcgt ctggcacgac agccacgcgc accccgtacc gcgcccggtg ctcgacatcc
     5701 tgaccggcct cgccacccgc gtcgcaccac ccggggtgct gctggaacgg gacgacgact
     5761 tccccggacc ggccgaactg gagcgggagt tgcacaccat ccgggatgcg gtggcggccg
     5821 ggcggtcccg ctcggcgggc ggtacgacgc cgacgggtac ggcgacgggg ggagccgtcg
     5881 gcacgggggc cgggggcgtc ggtgtcctgg cgccgcccgt ggccggggtc cgtgagcggc
     5941 tcggggtggc ccagaccgcc gtgctgtcct cactggtcgc ggggacgccg gtgccggagg
     6001 ggttcgaccg ggcgcggatg cgggtgcagg cgcgggccct ggcggcgaag cgggcggacg
     6061 tggtggcgaa ggtggcgccc gagctgccgg tgatcctggg ggcggagtac cgggaggcgt
     6121 tcctggcgta cgcacggcag cggccgatga ccgccggata ccggcgcgac gcgctggatt
     6181 tcgcgggcca cctgctgaag ctgggccgcc cggggaacgc ggggacacgg cgcagactgc
     6241 ggcagtggtg gctggaccga gcgggcccgg ccccgcggcg cccctcaccg acgaccaggg
     6301 cactacgggc actgctacga cgccgagcct gacgagacgc cgggcccgac gggagaccaa
     6361 gcctgacgag cggctcaccg acggcacagc cgataccggg ggaccacgac ggcgccgaaa
     6421 ccagcgacag gaccgcccac gaccccccga cacctcccgg cccgaggcca ccaccgactc
     6481 cccaggggcg cggggacctg cgcgaccggc caccccacca cgcgcacccc agcacccacc
     6541 ccgcccacga cggcgccagg gaccgaccac cccgcccctc accggcgccg cggacggcgt
     6601 ccctccgcct cgcagtaata tgccaagccc gcagccaccc cccaagcaca ccacggcgtg
     6661 cggtgcagga ggcagcatgc gaccgcgacc cccgatcgag ggccgaggca tattcagcgg
     6721 caccggactc gtcgtcgccg ctctggcggc gacggtcgtc gcgctgctcg tcccgctctg
     6781 gtcgtacgcc ggccggcagg acccggcgag cgcgaacgtg ctgagtgcgg cgacggtgac
     6841 gaccccgtac ggtccgctgt ccgcgcggga ccgggacttc gtcaccaagg tgcggctggc
     6901 cggactgtgg gagctgcccg ccggggagct ggcgcagcgc aagggcacga cgccggcggt
     6961 ccgcacggcg ggacggcacc tcgtcgacgg ccacacctcg ctggacgcgc acgtgcggac
     7021 cgtcgccacc cagctcggcg tggccttgcc caacgaaccc aacgcccagc agaagcagtg
     7081 gctggccacc ctggacgcgg cgcggggcca ggacttcgac cgccagttcg ccggccttct
     7141 gcggctcgcg cacggccggg tgttctccct ggtcgcagag gtgcgggcgg gtacgcagaa
     7201 ctccctcgtc cgtgatctcg ccgacgacgc caacaccacc gtgctggacc acatcaagat
     7261 cctggaggcg acggggtacg tcgacttctc ggcgctggcc cgggacctgg cgagcccggc
     7321 gtccccggtg ccgacgccgc cggaggccga ccccggtgcc gcggtgccgg tcacgccgtc
     7381 gccgggtgag acgtacgccc tgccgccggc caccgccggc ccgccgccgg cggcgagcgg
     7441 tccctgacgg cggccggccc gcccgggaaa cggagcgcgt cacacgaaac ggaacgtcac
     7501 ataccggcga cactgtgtcc ggatcgtgaa cagggcatgg cgtccgcccg gggccctggc
     7561 atagaaacag ctcatgttct gggtccttct cctgctcctg gcctgggctt tcgccggtac
     7621 ggcctgcgcc ctgctgtgcc tggcggccct ccgcgcggcg accgcggaag cgcgtgacgc
     7681 gtcgacggac cgggagcgcg gtctgtcgct gtacgaggcg gccttcctgt ccggcggccc
     7741 cgcgcgggtc gccgacgtca ccctggtgtc catggcccgc cagcggcggc tgctgctcgc
     7801 gcacaccggc tgggcgacgg tcgtcgaccc gcggggccgc gacgagatgg agcggtcggt
     7861 cataggggcc atagggcccg agggccagtc acggatcgcg ccggtgcggg cacaggccgc
     7921 cggcgccgag gcggtgcgcc ggctggccga cgggctggag cgcgcggggc tcgcggtgcc
     7981 ggagggcacg ggggtcaccg tgggggccgc cgtccgccgg gtgcgggccg ccgcggtggc
     8041 cgtgccggcg ctgggcgcga ccgcgctgct gctgcccgtc cagaccggga cgccgcgcct
     8101 gctcgtgggc ctgtggttcg ccctgcccct gacgctgtcg ctgagctgcc tggccatcgc
     8161 ccggttcgag gtgcatccgt actcgcgctg ggcctccccg gccgggcagc ggctgctggg
     8221 cgcgctggcc ggcaggcggg acggtgacga gcggtcgttc ctcacgtccg tcgccgtacg
     8281 cggcatccgc gcgatcggcg aaccggagct gcgcgcggcc ttcacccacc gcgaccagcc
     8341 ctggcgggaa tgaggcctcc tcccccggag tggaggctcc ttccgggggg cgcacggggg
     8401 gcattgccgc cgacaccggc cggtcggtgc ttgccttcgc cggcagccgg ccgaagcatc
     8461 ccttgtgacg tcgccgggcc gtggcagccg tgccatcgcc gccgcgcacc gaagggacac
     8521 gcgatgaaag ctgccgccct ctactcggcc gccgggtcct tgctgctgac cagcctggcc
     8581 gcggccccgg cgggcggcgc gcccggcagc cccggcgcct tcggcgctcc cggcgcggcc
     8641 gagctgcgcg ggaccgcggt ggcggtggcc cgggcccggg cggccggtat cgccttcggc
     8701 ccgtgcgcgg cggcggacgt gccgcaggcc ccggccgagc tacggtgcgg cacggtcacc
     8761 gtcccgctgg actacgccca ccccgacggc aggaagatcg aactgacggt cagccggacc
     8821 cgggccaccc agaaggaccc gcacaacagc aagcggaagg tcccccggca gggcgccctc
     8881 gtctacaacc ccggcggtcc gggcgcctcc agcatgtact tcccgctgat cggcaagatc
     8941 ccgcagtgga agcggaccgc cgccgcctac gacctggtcg gttacgcccc gcgcggggtg
     9001 ggccgttcgg cgccgctgtc ctgcgaggac ccgaagcact tcttcaaggc gcccaccgcg
     9061 tcaccggcgt tcccgtcgca ggcgttcaag cggcagcgcg tcgcgcaggc gaaggcgtac
     9121 gcgcgcggct gcgcccagcg gtccgggagc gggctgcggt tctacacctc cctcgccaac
     9181 gcccgggacc tggacgtcct gcgggccgcg ctgggcgagg accggctgac gttcatgggc
     9241 gcgtcgtacg gcacctactt cggcgcgctc tacgcgacga tgttcccctc gcacgtgcgg
     9301 cgcatggtgc tggactcggc ggtgaacccg gacccggaga aggtctggta ccgcaacaac
     9361 ctcgaccagt cggccgcctt cgaggagcgc tgggcggact tccgcgagtg gatcgcccgg
     9421 cacgacgacg tgtacgggct cggtgacacg cccgcgaagg tgcagcgcgc ctacgacacc
     9481 gcgcgtgagc ggctggccgg gaaggcggcg ggcggcaggg tcggcccggg gcagctacag
     9541 aacgcgttcc tgtcggccgg gtactacgac gacttctggc cgagccgggc cgaggccctc
     9601 gcggcgttcc tgcgcggcga cccggagccg ctggtgcggc tggccgcgcc gagcccggac
     9661 acggccgtgg aggcggagaa cggcagagcg gtgtacacgg ccgtcgagtg caacgacgcg
     9721 ccgtggccga cgcggtggaa ggtctgggac cgggacaaca cgcggctcgc ccgggtggcg
     9781 cccttcgaga cctgggacaa cgcgtggatg aacctgccgt gcgcctactg gccggcgccc
     9841 cggcagcgtc cgctggacgt gcggaccgct ccgggcgagc tgccgccggt gctgatcctg
     9901 gcggcccagc gggacgccgc ggcgccctac cagggggccc tggagacgaa ccggcggctg
     9961 gctgggtcgg tgctggtgac cgagcgggac gcgggcacgc acggcgtcgg gggcggcccc
    10021 aacccgtgcg tcaacgcgta cctggacgcg tatctgctcc agggccgcct tccggcgcgg
    10081 cacacgtcct gcgcaccgcg cccggaaccg gagccggaca ccccggccgc cggcgccccc
    10141 gctcccggct cgacctcgcg ggacgccgtg aagggcaggg ccgccgggcg ctaggcgttc
    10201 cgggcgggcc gcgccgggca ctgggcgttc cgggcggacg ccggccgtca ggccaggccg
    10261 gcgaccaggt cggcgacgtc cttgcggcgg cccgtgtaga acgggacctc ctcgcggacg
    10321 tgcatccggg cctcggaggc gcgcaggtga cgcatgaggt cgacgatgcg gtacagctcg
    10381 tcggcctcga aggccaggat ccactcgtag tcgccgaggg agaacgaggc gacggtgttg
    10441 gcgcgcacgt cggggtagcc gcgggccatc ttgccgtggt cggcgagcat gcggcggcgg
    10501 tcctcgtcgg gcagcaggta ccagtcgtag gagcgcacga acgggtagac gctgacgtag
    10561 tcgcggggcg tctcgtcggc gaggaacgcc gggatgtgcg agcggttgaa ctcggcgggg
    10621 cggtgcagcg ccatgttcga ccacaccggc tccagcgcgc ggcccagccg ggtgcggcgg
    10681 aagaggttgt acgcctcctg gagctgttca ctggtctcgg cgtgccacca gaccatgacg
    10741 tcggcgtcgg cgcgcaggcc ggagacgtcg taggtgccgc ggaccgtgac gtccttcgcg
    10801 gcgagctggt cgaacagctc ctggacctcg tcggcgtatc ccgtgcggtc ctccggcagc
    10861 acgtccttca gccggaacac cgaccacagg gtgtagcgga tgacctcgtt caggtccttg
    10921 gccagcttgc ccttgttcgg gaccctgccg gactcgatga tgggggcgtc gtcactcatg
    10981 gtccctattc tcccgctccg ccgtgcaggc tctgcaccgg gtcggcggtc agctcggcga
    11041 cgccgctcgg gtcgccgtcg agctggtcga ccgcggcgta ggcgctcgcg atgcacgcgg
    11101 ggatgccgac cccgtcgtac tgggcgccgc acaccgcgag gcccgggagc cgggcgacgt
    11161 gttcgcggat gcgggccacg cgcgcgtggt gcccgacggg gtactgcggc aggccgtcgg
    11221 tccagcgggt gacccgggtc tccaggggct cggcgtccag gccggtcgcc tcgcgcaggt
    11281 cgtgccggga gatctccacg agggcggcgt cgtcccggtc gagcacccgc gt
//
