LOCUS       NZ_RAII01000001        11056 bp    DNA     linear   CON 15-MAY-2024
DEFINITION  Streptomyces sp. E1N211 s-211_S3_L001_R1_001, whole genome shotgun
            sequence.
ACCESSION   NZ_RAII01000001
VERSION     NZ_RAII01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. E1N211
  ORGANISM  Streptomyces sp. E1N211
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7318313)
  AUTHORS   Vicente,C.M., Thibessard,A., Lorenzi,J.N., Benhadj,M., Hotel,L.,
            Gacemi-Kirane,D., Lespinet,O., Leblond,P. and Aigle,B.
  TITLE     Comparative Genomics among Closely Related Streptomyces Strains
            Revealed Specialized Metabolite Biosynthetic Gene Cluster Diversity
  JOURNAL   Antibiotics (Basel) 7 (4), 86 (2018)
   PUBMED   30279346
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7318313)
  AUTHORS   Leblond,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2018) Dynamique des Genomes et Adaptation
            Microbienne UMR 1128, Universite de Lorraine - Institut National de
            la Recherche Agronomique, Faculte des Sciences et Technologies
            Boulevard des Aiguillettes BP 70239, Vandoeuvre-les-Nancy F-54506,
            France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics Workbench v. 6.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003671735.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 02:33:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,941
            CDSs (total)                      :: 6,866
            Genes (coding)                    :: 6,131
            CDSs (with protein)               :: 6,131
            Genes (RNA)                       :: 75
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 736
            CDSs (without protein)            :: 735
            Pseudo Genes (ambiguous residues) :: 0 of 736
            Pseudo Genes (frameshifted)       :: 138 of 736
            Pseudo Genes (incomplete)         :: 673 of 736
            Pseudo Genes (internal stop)      :: 17 of 736
            Pseudo Genes (multiple problems)  :: 87 of 736
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:49:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6210177
            Orig. end                         :: 6221233
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            RAII01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11056
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="25"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11056
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11056
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:6056](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6056
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(81..1022)
                     /locus_tag="EBH12_RS29615"
     CDS             complement(81..1022)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1067: putative ABC
                     transporter permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436171.1"
                     /locus_tag="EBH12_RS29615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sugar ABC transporter permease"
                     /protein_id="WP_048458444.1"
                     /transl_table=11
                     /translation="MTATTTAPPAAPKTSAAPGRPSARLRAWATRAPLLPALIFMIVVT
                     QLPFVATLVISFFDWNSLYPDARHFAGFANYQEVLTDPALRKSVWTTVLLTVGVVLASL
                     VIGLLLALLLDRRFRGRAVVRTLLIAPFLVVPVAAALLWKHVLYNPEYGLLNGLLHYVG
                     GPQPDWISGTPLLAVQASLVWQWTPFMMLILLAGLQSRDQQQLEAARVDGASDWQIFVH
                     ITLPHLRRYLELGALLGSIYIVQNFDAVFTITSGGLGTANLPYTVYQTFYQAHENGLAS
                     AAGVLVVIGSIVIATFALRVVSSLFREEVSRA"
     gene            complement(1019..2389)
                     /locus_tag="EBH12_RS29620"
     CDS             complement(1019..2389)
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1143: sugar-binding lipoprotein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006381715.1"
                     /locus_tag="EBH12_RS29620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="WP_048458445.1"
                     /transl_table=11
                     /translation="MRTQSRRRPRGVLATAAAGTLIAPLLSGCWVGAGGAGSGGDAINV
                     LMVNNSQMVELQKLTAAHFTQETGIKVNFTVLPENDVRDKISQDFANQAGQYDVATLSN
                     YEIPIYAKNGWLKEMDSYVAEDRAYDQQDILEPMRQSLTGEDGKLYGQPFYGESSFLMY
                     RKDVFAEKGLTMPERPTWQQVADLAAKADGARPGMKGICLRGLPGWGEVMAPLTTVVNT
                     FGGTWFDEDWKARLDSPEWKEAVNFYVDLVREHGESGAPQAGFAECLNNLTQGKVAMWY
                     DATSAAGSLEADGSPVAGKIGYAPAPVEKTESSGWLYTWAWGIQNASRNPDNAWKFVSW
                     ASSKEYEQLAGEQIGWSNIPAGKRASTYANPDYRAEASAFQEMTKRAIEQARPDDPGVQ
                     PRPAPGIQFVGIPEFTDLGTKVSQEISAAIAGRQSVETALRKSQQLAEEIAEEYEGR"
     gene            complement(2493..>2858)
                     /locus_tag="EBH12_RS29625"
                     /pseudo=""
     CDS             complement(2493..>2858)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381184.1"
                     /locus_tag="EBH12_RS29625"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="transcriptional regulator"
                     /pseudo=""
                     /transl_table=11
                     /translation="SDNASVLLLGGRVRSGTLATVDHWTTKMLSGFVIDLAFIGANGIS
                     REHGLTTPDPAVSEVKAQAVRAARRVVFAGVHTKFGAVSFCRFAEVGALEAIVTSTLLP
                     ASEAHRYSLLGPQVIRV"
     gene            <2871..4214
                     /locus_tag="EBH12_RS29630"
                     /pseudo=""
     CDS             <2871..4214
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017947764.1"
                     /locus_tag="EBH12_RS29630"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="AfsR/SARP family transcriptional regulator"
                     /pseudo=""
                     /transl_table=11
                     /translation="LRCAGQRSWLARLDAEAGNLRAALDEAVRRAAAGQPDEALRLATA
                     LSWWWLLRGRLTEARRSLSTVLAAVPSAPELTLLHAGFALLTGDRTEAAATAAADAVPD
                     PVRRARALWLCAYGLFSAGEVTAGAELNATALELFDRAGEQWGTAAALGLRAALALVGG
                     DLDALGRDGRRSAQLFGELGDRWGELQTVSPLAALAEIKGEYDEAARLQREGLRLAGEL
                     GLEAEVSARLSGLGRLALLARDWERGRSLHERARRIAAEQGYKYGEIHSVMGLALGARR
                     SGDLDAAEAHLARLRDGYADVSSEAGDHLLLAELGFLAELRGDAERSVRHHLHGLDVAR
                     SLGEPRALALSLEGLAGAAALRGSRPAVACAALLLGAADAARRQVGAPLPPAERGDADR
                     VTATARTVLGAPAFAEAFERGARLTVEEAVTHVCAALDWPGRGTAETR"
     misc_feature    2988..2990
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(4218..4895)
                     /locus_tag="EBH12_RS29635"
     CDS             complement(4218..4895)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983710.1"
                     /locus_tag="EBH12_RS29635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_048460854.1"
                     /transl_table=11
                     /translation="MEKSGESSGPRLPVGPRELKQERAVRTRERILAAAATAFAAKGFP
                     AVTILDVAETAGMTKGAVYFHYENKEALALAVTERFYLRLEEIADTVEPLGLPPLGSVA
                     ELLLRTATAFRDETLVQAGARLQLERHLIGVRLPLPYEAYTGLITSWLTKEHEADTRGG
                     LPPPGTLATVLVSAFFGAQHISWVLNDRADLVKRTTDLVRTIIPSASEDMKSCPSAPRG
                     EHG"
     misc_feature    4911..4924
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.03"
                     /tool="antismash"
     misc_feature    complement(4912..4925)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.61"
                     /tool="antismash"
     misc_feature    complement(4923..4943)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.42"
                     /tool="antismash"
     misc_feature    4930..4945
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.41"
                     /tool="antismash"
     misc_feature    complement(4931..4944)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 20.02"
                     /tool="antismash"
     misc_feature    4931..4944
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.63"
                     /tool="antismash"
     gene            5001..6056
                     /locus_tag="EBH12_RS29640"
     CDS             5001..6056
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017947762.1"
                     /locus_tag="EBH12_RS29640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_114873610.1"
                     /sec_met_domain="AfsA (E-value: 7.8e-20, bitscore: 69.4,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQQSRLRPEPRGAQGRSGPELDFQRTVSRRLVHRAAVAEVFVTDA
                     LVLGEDRFLVGGQWPRDHALYHPDAQRGSDPLLFAETVRQALVYLAHWHYEIPLGHRFV
                     GYDMDFEITDPVALRVGGRPVPVELDASWEWTDRRPPRRYGARLEVTLSVGGRACGRGS
                     LRIVALDERSYRIIRGPQGGACAAPVAVEPRRRVEPALVGRLRNKDSVLAAGPSPAEWL
                     LQPDLDHAILFDHPTDHLPLMVLLEGFRQLGHLMTCSAAPRPEGRAPALVSAAVDCLAF
                     AELDVPTRLCVREDRTVDDVSAGDARTGRRLLRLDALQGDTLVASCASVWAPVSLATAT
                     GRPGGQSVSHA"
     misc_feature    complement(5041..5055)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 24.85"
                     /tool="antismash"
     gene            6504..7445
                     /locus_tag="EBH12_RS29645"
     CDS             6504..7445
                     /GO_function="GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1191: acyl-CoA dehydrogenase type 2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017947761.1"
                     /locus_tag="EBH12_RS29645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_241563436.1"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 6e-07, bitscore:
                     28.4, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLRAAAVVGEGCASAAWCAVLWAAHGRFAALLPEEGQKEIWGEGP
                     DVRIAAALMPPSGTALRVPGGWSLQGEWGLVSGVDHADWVLLAAPETGRPGGAARVFAV
                     PADLVAVRDTWNATGLRATGSNTVVLEATVVPDGRSVPLAALLAGDSTDGVPPCHAAPA
                     HLAGGVMFCAPAVGAARRVLAVWSGWAQGAAPGRVRPLDRPSVQERLARSAAEIEAAGL
                     LLGAAAGRADGGAITAEATAVNRRDVAIAADWLATSVDRLFRTGGAHLRDTEGELHRHW
                     SDVLTIASHGALSLEPAAEAFARSLDGGADGA"
     gene            7438..8133
                     /locus_tag="EBH12_RS29650"
     CDS             7438..8133
                     /EC_number="3.1.3.-"
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017947760.1"
                     /locus_tag="EBH12_RS29650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HAD family hydrolase"
                     /protein_id="WP_048460855.1"
                     /transl_table=11
                     /translation="MPDIPGPAPGRLVFSDVDETLLSGKSLFDFLGFYFEGRYGADGAL
                     HATATRRRIAALVAAGLPREAGNRAYYEAWRGERLDAVTDWGIRWFARNAGAPGFYVPA
                     VRAALRRHREAGDHVVLVSGSFPAVVDPVAADVGARHVLCTRPEARDGVLTGVVAGEPC
                     IGEGKRRAVRGLAKRYPDVSLSDCYGYGDHHSDLPMLTEVGHPVVVGGSPELLAELPDA
                     RVLAGAESA"
     gene            complement(8173..8763)
                     /locus_tag="EBH12_RS29655"
     CDS             complement(8173..8763)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /locus_tag="EBH12_RS29655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_114873608.1"
                     /transl_table=11
                     /translation="MAKQERSSRTHERFLDASADEFVRHGYAGANLQRIATDVGMTKGA
                     LYAHFPSKDALAAAFTSAFNRVGHELLREVTEGDPPVKSLRHVTLGFMLRMQSDLRFRA
                     GVRLALDDGLAHQRVPAVVAELSAMLTRLVGDAQEKEELTTRQCPELISSLVIAVIFGA
                     YCTAPATGLDKGVERVRLLWQLLSLDAPGRAAP"
     misc_feature    complement(8962..8981)
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 23.22"
                     /tool="antismash"
     misc_feature    9107..9123
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.84"
                     /tool="antismash"
     gene            9131..9412
                     /locus_tag="EBH12_RS29665"
     CDS             9131..9412
                     /GO_component="GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018958688.1"
                     /locus_tag="EBH12_RS29665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="WP_234309985.1"
                     /transl_table=11
                     /translation="MSEPVIESMAEVRGHLADVIDRARREETPTIITRRGKQEAVVIDI
                     DEYQRLRRIAEDAEEAWLNRLADEAESEGLEGAVSLEEMAAMLRTDRD"
     gene            9417..9698
                     /locus_tag="EBH12_RS29670"
     CDS             9417..9698
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]"
                     /GO_function="GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /GO_process="GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010355355.1"
                     /locus_tag="EBH12_RS29670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_114873607.1"
                     /transl_table=11
                     /translation="MGYVTRFTPHAQRDMLKIPRPDALRILYRLTELQKAMDAGDTAAF
                     DIKALQGHSARWRLRVGDYRVVYTVEDGRLIVWVLALGNRRDVYRQVP"
     misc_feature    complement(10221..10235)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.52"
                     /tool="antismash"
     misc_feature    complement(10245..10256)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.72"
                     /tool="antismash"
ORIGIN
        1 cacgatgccg gccagccagg cgaccaggcc gagacccgct ccgccgcgga accggccgcg
       61 ggtgcgtacg gcggtgctgc tcatgcgcgg gacacctcct cgcggaacag ggacgagacc
      121 acgcgcaggg cgaaggtcgc gatgacgatg gagccgatga cgaccaggac gccggccgcc
      181 gaggcgaggc cgttctcgtg cgcctggtag aaggtctggt agacggtgta gggcaggttg
      241 gcggtgccga gaccgcctga cgtgatggtg aacaccgcgt cgaagttctg cacgatgtag
      301 atcgagccga gcagggcgcc cagttcgagg tagcggcgca ggtgcggcag cgtgatgtgc
      361 acgaagatct gccagtcgct cgcgccgtcc acgcgggccg cctccagctg ctgctggtcc
      421 cggctctgca gcccggccag caggatgagc atcatgaacg gcgtccactg ccacaccagc
      481 gacgcctgca cggcgagcag cggggtgccg gagatccagt cgggctgagg gccgccgacg
      541 tagtgcagca ggccgttcag caggccgtac tccgggttgt agaggacgtg cttccagagc
      601 agggcggccg cgaccggcac caccaggaac ggcgcgatga gcagggtgcg cacgacggcc
      661 cgcccccgga acctgcggtc cagcagcagc gcgagcagca gtccgatcac caggctggcc
      721 aggaccacgc ccacggtgag caggacggtc gtccagacgg acttgcgcag cgccgggtcg
      781 gtcagcacct cctggtagtt ggcgaacccg gcgaagtgcc gggcgtcggg gtagagggag
      841 ttccagtcga agaaggagat caccagggtc gccacgaacg gcagctgggt gaccacgatc
      901 atgaagatca gggcgggcag cagtggggcg cgggtggccc aggcgcgcag ccgggcggag
      961 ggccggcccg gtgcggcgga cgtcttcggt gcggccggag gggccgtggt cgtggcggtc
     1021 atcgtccctc gtactcctcg gcgatctctt ctgcgagctg ctgggacttc ctcagggccg
     1081 tctcgacgga ctggcgtccg gcgatggccg cgctgatctc ctgggagacc ttggtgccga
     1141 gatcggtgaa ctccgggatg ccgacgaact ggatgccggg cgccggacgg ggctggacgc
     1201 cgggatcgtc ggggcgggcc tgctcgatgg cccgcttggt catctcctgg aaggcggagg
     1261 cctccgcccg gtagtcgggg ttggcgtagg tggaggcgcg cttgcccgcg gggatgttgg
     1321 accagccgat ctgctcgccg gccagctgct cgtactcctt gctggacgcc caggagacga
     1381 acttccaggc gttgtccggg ttgcgggagg cgttctggat gccccacgcc cacgtgtaga
     1441 gccatcccga cgactccgtc ttctcgaccg gcgccggcgc gtagccgatc ttccccgcga
     1501 cgggggagcc gtcggcctcc agggagccgg ccgcggacgt ggcgtcgtac cacatggcga
     1561 ccttgccctg ggtgaggttg ttcaggcact cggcgaaacc ggcctggggc gcgccggact
     1621 cgccgtgctc ccgtaccagg tccacgtaga agttgacggc ctccttccac tcgggggagt
     1681 cgagccgcgc cttccagtcc tcgtcgaacc aggtgccgcc gaaggtgttg accaccgtgg
     1741 tgagcggtgc catgacctcg ccccagccgg gcagtccgcg caggcagatg cccttcatac
     1801 cgggccgggc gccgtccgcc ttcgccgcga ggtcggccac ctgctgccag gtgggccgct
     1861 cgggcatcgt caggcccttc tccgcgaaga cgtccttgcg gtacatcagg aacgacgact
     1921 cgccgtagaa cggctggccg tacagcttgc cgtcctcgcc ggtgagggac tgccgcatcg
     1981 gctcgaggat gtcctgctgg tcgtacgccc tgtcctcggc gacgtaggag tccatctcct
     2041 tcagccagcc gttcttggcg tagatgggta tctcgtagtt ggacagggtg gccacgtcgt
     2101 actggccggc ctggttggcg aagtcctggc tgatcttgtc gcggacgtcg ttctccggca
     2161 gcacggtgaa gttcaccttg atgccggtct cctgggtgaa gtgggcggcg gtcagcttct
     2221 gcaactcgac catctgcgag ttgttcacca tcaggacgtt gatggcgtcg ccgccggatc
     2281 cggccccgcc cgctccgacc cagcagccgg agagcagcgg ggcgatcagc gtccctgcgg
     2341 cggccgtggc gagtacgcct cgtggcctcc gtcggctctg ggttcgcatg gatcgctcct
     2401 ggacgtatag ggagacatcg ggcgtcacct ggcatggctc ggccccgtgg ggacgtggaa
     2461 acggtgtgcg gttctgtgcg tggggccgcc cgtcagaccc tgatgacctg cggtccgagc
     2521 agggagtagc ggtgggcctc ggaggccggc agcagggtgc tcgtcacgat cgcctccagt
     2581 gcgccgacct cggcgaaccg gcagaagctg accgctccga acttggtgtg gacccccgcg
     2641 aacaccacgc ggcgggcggc ccgcaccgcc tgcgccttga cctcgctgac cgccgggtcg
     2701 ggcgtggtca ggccgtgttc gcgggagatc ccgttggcgc cgatgaacgc caggtcgatg
     2761 acgaagccgg agagcatctt cgtcgtccag tggtccacgg tggcgagcgt cccggagcgg
     2821 acgcggccgc cgagcagcag gacggacgcg ttgtcgctcg cggtgcgccg ctgcgctgcg
     2881 ccggtcagcg gtcctggctc gcccggctgg acgcggaggc cggcaatctg cgcgcggcgc
     2941 tcgacgaggc ggtgcgccgc gctgccgccg gacagcccga cgaggcctta cggctcgcaa
     3001 ccgccctgtc ctggtggtgg ctgctgcgcg gccggctcac cgaggcacgc cgcagcctgt
     3061 cgacggtcct ggccgctgtt cccagtgctc ctgaactcac cctcctgcac gcggggttcg
     3121 ctctgctgac cggcgatcgc accgaggcgg cggccaccgc ggcggccgac gccgtgcccg
     3181 atcccgtacg ccgcgcccgt gccctctggc tgtgcgcgta cggcctgttc agcgcgggcg
     3241 aggtgaccgc cggagcggag ctgaacgcca ccgcgctcga actcttcgac agggcggggg
     3301 agcagtgggg caccgcggcg gcgctcgggc tgcgggccgc gctcgcactg gtcggcggcg
     3361 acctcgacgc actggggcgc gacggtcggc gcagcgctca gctcttcggc gaactgggcg
     3421 atcgctgggg ggagttgcaa accgtctcac cgctcgccgc gctcgctgag atcaagggcg
     3481 agtacgacga agccgcccgc ctgcagcggg aagggctgcg cctggcgggg gaactgggtc
     3541 tggaggccga ggtgtcggca cggttgtccg ggctcgggcg gcttgccctg ctcgcccgcg
     3601 actgggagcg gggccggagc ctgcacgagc gggcccgtcg catcgccgct gaacagggtt
     3661 acaagtacgg cgagatccac tcggtgatgg gcctcgcgct cggtgcgcgc cgctccggtg
     3721 acctcgacgc ggccgaggcc cacctggcgc gcctgcgcga cggctacgcc gacgtctcct
     3781 cggaggcggg cgatcacctg ctccttgcgg agctcggctt cctggccgaa ctccggggcg
     3841 acgccgagcg gtccgtacgc catcacctgc acggcctgga cgtggcgcgc tccctgggcg
     3901 agccgcgtgc gctggccctg tccctggaag gcctggcggg cgcggcggcg ctgcggggga
     3961 gccggcccgc cgtcgcttgc gccgcgctgc tcctcggggc ggcggacgcg gcccgccggc
     4021 aggtgggcgc cccgttgccg ccggcagaac gcggcgacgc cgaccgcgtc acggcgacgg
     4081 cgcggacggt actgggcgcc ccggctttcg ccgaggcgtt cgagcggggg gcccggctga
     4141 ccgtggagga agcggtgacc catgtgtgcg ccgcactcga ctggccgggc cgggggacgg
     4201 ctgagacccg gtgacggtca gccgtgctcc cctcgcggag ccgacggaca cgacttcatg
     4261 tcctcgctcg ccgacggaat gatcgtccgc accagatccg tggtgcgttt caccaggtcg
     4321 gcccggtcgt tgagcaccca tgagatgtgc tgggcgccga agaaggcgga gaccagcacg
     4381 gtggcgagcg tgccgggcgg cgggaggccg ccacgggtgt ccgcttcgtg ctccttggtc
     4441 agccaggagg tgatcagccc ggtgtacgcc tcgtacggca gcggcaaccg gacgccgatc
     4501 agatgccgct ccagttgcag ccgcgccccg gcctggacga gcgtctcgtc ccggaacgcc
     4561 gtggccgtcc ggagcaggag ttcggccacg gagccgaggg gcgggagacc caacggctcc
     4621 acggtgtcgg cgatctcttc gaggcggagg tagaaccgct cggtcaccgc cagcgcgagg
     4681 gcttccttgt tctcgtagtg gaagtacacg gcgcccttgg tcatcccggc ggtctcggcc
     4741 acgtccagga tggtgaccgc cgggaagccc ttggcggcga acgccgtggc ggcggccgcg
     4801 aggatgcgtt cccgcgtccg taccgcgcgc tcctgtttca gttccctcgg ccccacggga
     4861 agtcggggac cggaggattc acctgacttt tccatggtga cctcttttcg ctcttttgaa
     4921 gaaacctgct cgaaggtatg tttggcggcc cggggggaca ggctctgtcg gcctgctctg
     4981 acgtctcagg gggagaatcg atgcagcaga gccgacttcg gccggagccg cgaggtgcgc
     5041 agggacggtc gggcccggag ctcgacttcc agcggacggt gtcccggcgg ctcgtgcacc
     5101 gggccgccgt ggcggaggtc ttcgtcaccg acgcgctggt gctcggcgag gaccgcttcc
     5161 tggtcggcgg tcagtggccg cgcgaccacg cgctctacca ccccgacgcc cagcggggga
     5221 gcgaccctct cctgttcgcc gagacggtca ggcaggcgct cgtctacctc gcccactggc
     5281 actacgagat cccgctcggc caccgcttcg tggggtacga catggacttc gagatcaccg
     5341 acccggtcgc gttgcgcgtg ggcggccggc cggtgccggt ggaactggac gccagctggg
     5401 agtggaccga ccgccgtccg ccgcgccggt acggtgcccg gctggaagtc accctctccg
     5461 tcggtggccg tgcttgcgga cgcggcagtc tgcggatcgt ggcgctcgac gaacgcagct
     5521 accggatcat ccgcggcccc cagggcggag cctgcgccgc ccccgtggcc gtggagccgc
     5581 gccggagggt ggaaccggcc ctggtgggcc ggctgcggaa caaggacagc gtgctcgccg
     5641 ccggtccgtc gcccgccgag tggctgctgc agccggacct cgaccacgcc atcctgttcg
     5701 accaccctac cgaccacttg ccgctgatgg tgctgctgga gggattccgc cagttggggc
     5761 acctgatgac ctgttcggcg gcgccacggc ccgaagggcg cgcgcccgct ctcgtttcgg
     5821 cggccgtcga ctgcctcgcc ttcgcggaac tcgacgtgcc cacccggctc tgcgtccgtg
     5881 aggaccgcac tgtcgacgac gtgtcggccg gtgacgcacg gaccgggcgc cgactgctgc
     5941 gcctcgacgc gctccagggt gacaccctgg tcgcgagctg tgcctcggtg tgggctccgg
     6001 tgagcctcgc cacggcgacg ggccggccgg gcggacagtc cgtgtcccac gcctagcggc
     6061 tgagggccgg cgccgccgtc ccgcaccgtc gtcctgcacc atcccatgcc atgcctcctg
     6121 ctcccgcccc tgggcatggc cggcgaatcg cttacatacc gtcgagaagg aatcgtactg
     6181 gagcgtggcg gaacaccgaa ggttccgctg gtgctttctg cgccctcgac gtgctcaacc
     6241 tctgctgaca tgtggcttat cgagggaagg acgtcatgat cgaagctgat gtggcacgaa
     6301 ttcgagccgc gtcccacggc cggcccggga ggccggatcc gaaggctgtc cgaaaggcgg
     6361 ccgacgcggc gcgccgagac agcggcgcgg cacggcacga gcggcggctg acgtccggga
     6421 cgatcgccgc tctgcgggcc gcgggcttcg agagacactt cgtcccgcgg cgctggggtg
     6481 gcgaggaagg gacgttcggt gccgtgctcc gcgcggccgc cgtggtgggg gagggatgtg
     6541 catcggccgc ttggtgtgcc gtgctgtggg cggcccacgg ccggttcgcg gcgctgctgc
     6601 cggaagaggg gcagaaggag atctggggag aaggcccgga cgtccggatc gccgccgccc
     6661 tcatgccccc gtcggggacc gcgctccggg tgcccggagg ctggtcgctg cagggcgagt
     6721 ggggtctcgt cagcggcgtc gaccacgccg actgggtgct gctggccgca ccggagaccg
     6781 gccgcccggg aggggccgcc cgtgtcttcg ccgtcccggc ggacctcgtg gcggtccgcg
     6841 acacctggaa cgccacaggg ctgagggcca ccggcagcaa cacggtcgtc ctcgaggcga
     6901 ccgtggtacc cgacggccgc agcgtccccc ttgccgcact gctcgcgggc gacagcacgg
     6961 acggcgtgcc gccgtgccac gcggcgcccg cccatctcgc gggcggggtc atgttctgcg
     7021 ccccggcggt gggggccgcc cgtcgcgtcc tggccgtctg gagcggctgg gcccaggggg
     7081 ccgcgcccgg cagggtacgg ccgttggacc gcccgtcggt gcaggagcgt ctcgcccgct
     7141 cggccgctga gatcgaggcc gccggactgc tgctgggcgc ggcggcggga agggcggacg
     7201 gcggggcgat cacggcggag gcgaccgccg tcaaccggcg ggacgtcgcg atcgccgccg
     7261 actggctggc cacgtcggtc gaccggctgt tccgcaccgg cggagcacac ctgcgggata
     7321 ctgagggcga gttgcaccgg cactggtcgg acgtactgac catcgcctcc cacggcgcgc
     7381 tgagcctgga accggccgcc gaggccttcg cccggtccct cgacggcgga gccgacggtg
     7441 cctgacattc ccggtccggc ccccggacgg ctcgtgttca gcgacgtcga cgagaccctg
     7501 ctcagcggca aaagcctgtt cgacttcctg ggcttctact tcgagggacg gtacggagcc
     7561 gacggagccc tgcacgcgac ggcgacgcgg cggcgtatcg ccgccctggt ggccgccggc
     7621 ctcccgcgcg aggcgggaaa ccgcgcctac tacgaggcgt ggcgggggga gcggctggac
     7681 gcggtgacgg actggggaat caggtggttc gccaggaacg cgggcgctcc cgggttctac
     7741 gtgccggccg tgcgcgcggc gctgcgcaga caccgggagg ccggggacca cgtcgtgctg
     7801 gtgtcgggtt cgtttcccgc ggtggtcgat cccgtcgcgg ccgatgtcgg tgcccgccat
     7861 gtgctctgca cccgccccga ggcgcgcgac ggagtgctca ccggagtcgt ggccggtgag
     7921 ccgtgcatcg gggaggggaa gcgaagggcc gtacggggcc tggcgaagcg ctacccggac
     7981 gtcagcctgt ccgactgcta cgggtacgga gaccaccact cggacctgcc gatgctcacg
     8041 gaggtcggac acccggtggt cgtcggcggc agcccggagc tgctcgccga gcttccggac
     8101 gcccgggtcc tggcgggtgc cgagtcggcc tgaaccggtg gcctggcgga tggtcaccac
     8161 agccgctccg tatcacgggg ccgctcggcc gggagcatcc aaggacagca actgccacag
     8221 gagacgcacc cgttcgacgc ctttgtccag gccggtcgcc ggcgcggtgc agtacgcccc
     8281 gaagatcacg gcgatgacga gcgagctgat cagctccggg cactgccggg tggtcagctc
     8341 ctccttctcc tgggcgtcgc cgacgagccg ggtgagcatg gcggacagtt ccgcgacgac
     8401 ggcgggcacc cgctgatggg cgaggccgtc atcgagggcg agccggacgc cggcgcggaa
     8461 ccggaggtcg gactgcatcc gtagcatgaa gccgagcgtg acgtgacgca ggcttttcac
     8521 cgggggatca ccttcggtga cctcccgcag gagctcgtgc cccaccctgt tgaacgccga
     8581 ggtgaacgcg gcggcgaggg cgtccttgga cgggaaatgg gcgtagagcg cgcccttcgt
     8641 catgccgaca tcggtcgcga tgcgttgaag gttggcgccc gcgtagccat ggcgtacgaa
     8701 ctcgtcggcc gacgcgtcca ggaaccgctc atgggtccgt gaagaccttt cctgtttcgc
     8761 catgattccc ctttggagtc actcggtcac gcacaataac agtcgtcgga gaatcttttc
     8821 ctgttcggcg gcccgctcac aagcccggtc cgggacttcc acctccgtct cgcgctcctg
     8881 cccaccgggc cccgttcctt cgccgttcgc gtcgaggtca ccgactcccg gggcgagcgg
     8941 gtcccgccgc ggccgtgcgt cctccggccg gaggtccccg ccgtcgacga aaccggccgg
     9001 ggcctcctcc tggtcgccgc gctcgccgcc cgctgggact ggcacccacg gccgggcggc
     9061 ccgggcgaga cggactgggc ggagtacgag actgcgatcg aatcagatgt acatctgcta
     9121 catttgtgac atgagtgaac cggtgatcga atcaatggcc gaggtccgag gccacctcgc
     9181 cgacgtcatc gaccgtgccc ggcgggagga gaccccgacg atcatcaccc ggcgcggaaa
     9241 gcaggaggcc gtcgtgatcg acatcgacga gtaccagcgg ctgcgccgga tcgccgagga
     9301 cgccgaagag gcttggctca accggctggc cgacgaagcc gagtccgaag gcctggaggg
     9361 ggcggtctcg ctggaggaga tggccgccat gctccgcacc gaccgggact gaccgcatgg
     9421 gatacgtcac gcgcttcacg ccgcacgcgc agcgggacat gctcaagatc ccgcgcccgg
     9481 acgccctgcg gatcctgtat cggctcaccg agttgcagaa ggcgatggac gccggcgaca
     9541 cagcggcgtt cgacatcaag gccctgcagg gccacagcgc ccggtggcgg ctcagggtcg
     9601 gtgactaccg cgtcgtctac accgtggagg acggccggct gatcgtgtgg gtcctggctc
     9661 tcggcaatcg ccgggacgtc taccgtcagg tgccctgacg tgccgtcccg cgaactaggt
     9721 acctgacgac gacgttcgcg cccgccgcgc ccgccgcgcc tgccgcgccc gccgcgcccg
     9781 ccgcggcctc aggtccgacg tcacccgccc gggagtcgta cgaggaaaat gcggcgacgt
     9841 cccccgggtc ccgcgccagg ttcttcgccg ctggtcgccg cgttgacgtg gtagcgcggc
     9901 accaacctgg cacgagatcg tgcgcaccgg gtcggtcttc caccagtccg tggaacgagg
     9961 ggggtggggg gtgtgcactg agattggttc cttcccacag cgcatgtgac attcgagcgt
    10021 ttccgacacc gaaaagggac gcaggattgg tgcatacctc ttgggtgtgg cgcggggggc
    10081 gggaattgtc cggcggcgct tccgacccgt ttcgtcaact cgcgggcgcc cccggccttt
    10141 accccctcac gggtggctca ctctcgcaca acccctgcgc ggccaacaag gcggtcgggc
    10201 aacatccccg cgttgacaga aagtcaaaaa gagaaggcgt cggtgaaacc gtgttccggc
    10261 attcgcggcg gccttttcca ggaggcggca cggttcttcg aaaagaggtg acgggaaggt
    10321 gtggccaagg tgtgatgtga aggcgcgtgc cactcccccg gtgtgccttt tccacggcgc
    10381 gcaccccgtg tgaacggcgc gtccgcaccg ccgggccgcg cacgctgccg gagcgcccgg
    10441 ggccacggtg cactggtggg acccttctcg gaggtcacgc gcggcgacgg cggcactctc
    10501 ccgcgcgccc ggccgccggc ccgctccctc ccgcccctcg gccggcggcc acgggaacgc
    10561 cccccccggg gccacggtgc cttgggggga cccttctcgg aggtcacgcg cggcgacggc
    10621 ggcactcccc cgcgcgcccg gccgccggcc cgctccctcc cgcccctcgg ccggcggcca
    10681 cgggaaggcc cccctccggg gcgggcgtca gatctgccag gaccgcagcc ggtcggcggc
    10741 gccgtacacg tcggtcgcgc cggagatcag gtcgcgcgcg aggtcgacca gggcaccgta
    10801 gggcgggtcg atgccggccc cgctgacgta catgtaggcg acggctgtgg cgcaggcgaa
    10861 gcgggcgttg gccgagggca gcgggcggag cacggcgagg gtgtgcagca gggcggcggc
    10921 ccgccaggcg gcgtccgagt tcacgccgag ccggggcggg tcgacgcggt gccgggcgac
    10981 ggccgcgacg agggcggaga agtcgttgac ggtgggctgg tccgggagga cctcctcgtg
    11041 ccgctggagc agccag
//
