LOCUS       NZ_RAII01000001        10876 bp    DNA     linear   CON 15-MAY-2024
DEFINITION  Streptomyces sp. E1N211 s-211_S3_L001_R1_001, whole genome shotgun
            sequence.
ACCESSION   NZ_RAII01000001
VERSION     NZ_RAII01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. E1N211
  ORGANISM  Streptomyces sp. E1N211
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7318313)
  AUTHORS   Vicente,C.M., Thibessard,A., Lorenzi,J.N., Benhadj,M., Hotel,L.,
            Gacemi-Kirane,D., Lespinet,O., Leblond,P. and Aigle,B.
  TITLE     Comparative Genomics among Closely Related Streptomyces Strains
            Revealed Specialized Metabolite Biosynthetic Gene Cluster Diversity
  JOURNAL   Antibiotics (Basel) 7 (4), 86 (2018)
   PUBMED   30279346
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7318313)
  AUTHORS   Leblond,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2018) Dynamique des Genomes et Adaptation
            Microbienne UMR 1128, Universite de Lorraine - Institut National de
            la Recherche Agronomique, Faculte des Sciences et Technologies
            Boulevard des Aiguillettes BP 70239, Vandoeuvre-les-Nancy F-54506,
            France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics Workbench v. 6.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003671735.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 02:33:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,941
            CDSs (total)                      :: 6,866
            Genes (coding)                    :: 6,131
            CDSs (with protein)               :: 6,131
            Genes (RNA)                       :: 75
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 736
            CDSs (without protein)            :: 735
            Pseudo Genes (ambiguous residues) :: 0 of 736
            Pseudo Genes (frameshifted)       :: 138 of 736
            Pseudo Genes (incomplete)         :: 673 of 736
            Pseudo Genes (internal stop)      :: 17 of 736
            Pseudo Genes (multiple problems)  :: 87 of 736
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:49:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3884857
            Orig. end                         :: 3895733
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            RAII01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10876
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="phosphonate"
                     /region_number="17"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     cand_cluster    1..10876
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10876
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5876](+)"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5876
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     misc_feature    complement(334..347)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     17.65"
                     /tool="antismash"
     misc_feature    579..593
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.65"
                     /tool="antismash"
     gene            complement(794..1351)
                     /locus_tag="EBH12_RS18360"
     CDS             complement(794..1351)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /locus_tag="EBH12_RS18360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_114872991.1"
                     /transl_table=11
                     /translation="MADIDLALQHYDGDAAKAIADELIDTYAEIYNVPPYADDPFFSVP
                     SYVDRLHAAFTMTGFETVTARLNGQLIGYVHGVTLPANKPWWTSLGDARPADAQAAAKA
                     EKIFWLRELMVRPAHTNHGVGRRLHDAVIAGRRDQPWTALTCIIDNEPAHGAYLRWGYQ
                     IMGRIKHAPESPVYDAMLLRPA"
     gene            complement(1378..2691)
                     /locus_tag="EBH12_RS18365"
     CDS             complement(1378..2691)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EBH12_RS18365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_114872990.1"
                     /transl_table=11
                     /translation="MHLTQEQNAEKLTKLAAAMYETGELTKRTSVSPRQFRRWESDAPP
                     WPHPDHRKLLERFYDRPIHELGFSPTGDTTPSTLAVTEPVPPVIRTEFETHDPVNRRQL
                     FATGAAALGSAAGTGLPWLSPTASAAPGDDELRVSHDDVENLRSAAHDLDALDQQFGSD
                     RLWRGARNQLLWLHHLIDHGTYSEPVGQELHAIAGQLTTSLGWFCYDADRQTEARVFFS
                     EALNTAMLDGDDALATRTLSNMARQSVDLGKAREAIRFSRIAQAHATEWSAPPRVAALL
                     AIREAQGHARLGDEISAGNAIKRAWNHFEHGTSQRDPDWTGFLNEAELTCLEGMCRLDL
                     GQHRQSVKLLDRSAQLQDIARSRNRGMCLARLAYAALQGHDLDHSVHAVDESLQLIEGG
                     MTSTRNIKQLTLVRNGLSLHKAHQPAREMAERLTAYTA"
     misc_feature    complement(2527..2529)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(2820..2835)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.61"
                     /tool="antismash"
     gene            3027..4328
                     /locus_tag="EBH12_RS18370"
     CDS             3027..4328
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /locus_tag="EBH12_RS18370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding protein"
                     /protein_id="WP_196421296.1"
                     /transl_table=11
                     /translation="MSVTVTRCPEPTDAHPRPAQPVSSRETTLEGTAKMAGCARRFLRR
                     TATAWSVDVDAVEAAEVVVSELFTNAVQHAQAERIRLRLRCQHALLHVEVFDPQPHGEL
                     VAARPGPDAEDGRGLEIVEALCLRWGRRPDADRCGSVTWACLPAPGAEPTQHDRAADPE
                     QPCEAGNLPRPVAMLPVHPAPVPFEGDLVPPSPPPPPTLYESWEVLADGLHVQRLALHL
                     PQLREQLTAQTAGTGSTVAALVARPAEVPTAQALALIDAAGLSAWVDLPAEHGRSDVAV
                     SPLASAAEDVHALLLPGVTDDALRGAVRVVAAASAWWVGAFAAIRHLGVHHSSLTPVKD
                     TVGSDTLREAVRVVALGTAQRVLAHQLRHDASEEAVRLAYCRAVTEGIVIEPTLPALLE
                     ELGELRLVDLVTTAIPWRGRFAKYAGGTGAGQVE"
     misc_feature    complement(3028..3048)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 21.77"
                     /tool="antismash"
     misc_feature    4333..4349
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 20.66"
                     /tool="antismash"
     gene            4333..5004
                     /locus_tag="EBH12_RS18375"
     CDS             4333..5004
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311999.1"
                     /locus_tag="EBH12_RS18375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AAA family ATPase"
                     /protein_id="WP_114872988.1"
                     /transl_table=11
                     /translation="MQNTRNPFIVVEGLDGTGKTTLRRGLFRLFEGLYRVTPLAVLTTN
                     FLDASVAADLVEGKYQPSADNQYRYLAALAADKQATATRLIAPSLLARPVIADRWLLSE
                     LAFFAVKHDRSPKETYPHLAEHLDVIPDLTLVLDISPEAAMERAAARSGDATRPDWDVL
                     DIQARVREVYQTVTAEPAAYPALGPVVRIDAAQDRATVLHTAWQALDQRGLLPKLPEGA
                     Q"
     gene            5001..5876
                     /locus_tag="EBH12_RS18380"
     CDS             5001..5876
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231: carboxyvinyl-carboxyphosphonate"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311939.1"
                     /locus_tag="EBH12_RS18380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="WP_241563235.1"
                     /sec_met_domain="PEP_mutase (E-value: 7e-48, bitscore:
                     161.9, seeds: 69, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 7.5e-60, bitscore:
                     201.0, seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTHMDVDISALKDRLAQARLARTPLRAVGAASAVAAKVAVEAGF
                     DALWVSGLEVSTALGLPDENVLGPRDLTDVVRALTRTAPLPVIVDVDNAGGQVATARRF
                     ADDLAHAGAAALCLEDSAYPKTNSFALHRTQELAKPVLMQEQLAVMRAAAGHEMLLIAR
                     TEALICGTDLPEAISRARAYEQAGADAVLIHSKDTTGRQALATAAAWTGAVPLVTVPTA
                     FPHLAPQRLGEAGFALAIYANHLSRAALAAMRQVAASFSATGSFTADGIPLSDVADLLQ
                     VADPAARACL"
     misc_feature    5061..5063
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            5987..6457
                     /locus_tag="EBH12_RS18385"
     CDS             5987..6457
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311905.1"
                     /locus_tag="EBH12_RS18385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_114872987.1"
                     /transl_table=11
                     /translation="MTALPRISVSVKAAIVHQDRILLMSYDDHSGFHYNLPGGKAKAGE
                     PLRDAVVRKVKDETGLRVRTSRLLFVVEYVPEQYNDEFGTVHKTQHNFLAELLDEDTAP
                     RKSDPPDPIQVGFEWVPLEEFEDKYLLPRVNQQLLNALAGDPADRDVLVDRW"
     gene            6460..7146
                     /locus_tag="EBH12_RS18390"
     CDS             6460..7146
                     /EC_number="2.1.1.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311988.1"
                     /locus_tag="EBH12_RS18390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="WP_114872986.1"
                     /transl_table=11
                     /translation="MHSRPASVLNLAELRDWLLDHHGNTVDADRLPAIRTELEHLTTRQ
                     VAGAVVELGCYQGAMALWMRAVLDATAQHERPVHVYDSFQGLPERSEQDPELFPAGFLR
                     AEPEQVLALHDRWGRTPPVIHPGWFADTLPDQLPDRIAFAYLDGDYYDSIRTCLTACVP
                     RMAPGGTLIVDDYADLEANPKAWNGLPGVKAACEDYFCTPSPLKVIVGAGDLAFGRYTA
                     PARAEQ"
     gene            7176..7715
                     /locus_tag="EBH12_RS18395"
     CDS             7176..7715
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /locus_tag="EBH12_RS18395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_241563621.1"
                     /transl_table=11
                     /translation="MVPGAADPVVLRRHLPGCAIRVIESLDTAALNLAARAEVLVLRSG
                     HRLDAERLTRWPKLRHVIRAGSGLDGLDVEALAARGVTVHRNPAPAADAVAEWALAALL
                     GLARRIPYGHAPLLRGQHTKQQCIAPPISRMRVAIWGAGPVGRACATALAPYVDEIAFA
                     ARPSPNSPSSWWTCWG"
     misc_feature    7482..7484
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            <7679..8089
                     /locus_tag="EBH12_RS18400"
                     /pseudo=""
     CDS             <7679..8089
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010040711.1"
                     /locus_tag="EBH12_RS18400"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="(Fe-S)-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="ELTEFLVDVLGVTDVGAYYPHRVTYHPTCHGLRSLGLGERPLRLL
                     RAVKGLELLELPGADECCGFGGTFALKNADVSAAMGADKVRNAEATGAEVLCAADNSCL
                     MHIGGTMTRLRTGMRPVHIAEILASTEEDPAV"
     gene            8086..9564
                     /locus_tag="EBH12_RS18405"
     CDS             8086..9564
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019521306.1"
                     /locus_tag="EBH12_RS18405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="WP_114875580.1"
                     /transl_table=11
                     /translation="MSGTFVGMPAFPAAARDAVRDTTLRGNLRHATHTIRAKRARAVAE
                     LDDWERLRQAGKRIKDRTLRHLDRYLVQLEESVTAAGGVVHWAADADEANRIVSELVKA
                     TGETEVVKVKSMATQEIALNEALEAQGIHAYETDLAELIVQLGKDRPSHILVPAIHRNR
                     GEIREIFAREMGEWGRPAPEGLTDTPADLAEAARLHLREKFLRAKVGISGANFMVAETG
                     TLVVVESEGNGRMCLTLPETLISVVGIEKIVPTWQDLEVFLQTLPRSSTAERMNPYTSM
                     WTGTTDADGPRAFHLVLLDNGRTDTLADEVGRQALRCIRCSACLNVCPVYERAGGHAYG
                     SVYPGPIGAILSPQLRGTGSEIDASLPYASSLCGACYEVCPVAIDIPEVLVRLRERVVE
                     GGEAVRDGNKVRLRPAKGHAAERAAMRAARWALTRPGALRTGQRLASHTRRLHPRTLPG
                     PGKAWSGTRDLPPVPAEPFRDWWRRTRGGKDGGA"
     gene            9561..10193
                     /locus_tag="EBH12_RS18410"
     CDS             9561..10193
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019521307.1"
                     /locus_tag="EBH12_RS18410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lactate utilization protein C"
                     /protein_id="WP_114875578.1"
                     /transl_table=11
                     /translation="MSSRERILGRVRRALADVPPDEDAPVERDYLREHGERTVEETVDL
                     LAENLGDYRAVVHRADSEELPYLIMRLLAQRGPQYVLVPPGLPPEWMSAADPTRVHDRA
                     VSTARELDQVESVVTGCAVAVAETGTIVLDGSPDQGRRRISLIPDHHICVVRVPDQVVA
                     SVPQALERLDPARPLTWISGPSATSDIELDRVEGVHGPRTLEVVLLR"
ORIGIN
        1 gcggggatgc cggtcacctc ggtgatgacc tcgctcgccc gcaggtcggt gtcgcgctcg
       61 atgcccttca tcagcgacac gatcacggtg tcgcgtccga tgtgcggcgc ccacgatgcg
      121 aggctttccc gaagggactg cgcagggatg gacagtacga ggaaatccgc accgtccagg
      181 gcggtggccg ggtcggtcgt ggccgtcagc gtcgggggca ggtccacgtc cgggaagtag
      241 ccggggttgc ggtgctcgcg gttgatcgcg tcggcgatct cgccacgacg ggcgtacgcg
      301 atcacgtcgg tgcccgcgtc agtcatgatc ttggcaatgg cggtgcccca ggatccggca
      361 gagaacaccg ccgcgcgggc ggggcggtgc tggctcactg cggggtcctt ccaagagggg
      421 tggcctgccg ccggggcagc ggtcgggtcc ggccacccta tcgattgggc ccgatgtctc
      481 cgggatcgca gtgcggcggc gccgagtgag caggacagcc cggcggtcgg agagggtggc
      541 agcatttcgt gtgcctcccc gaggatggcc aaagcgcgtg gagatcatca tcagccgtct
      601 cgtagctgtc cacgtgcacc agaaccttga ccgccgctgc tgcacctgga cgccgatcgc
      661 tgagatcctg ctcggcgcca ccgagaccga ttctgtgaag tcgcactgta gagataccgc
      721 tcaagcgcta tgggcggcgg gcatggcggt gctgccgcct gcggctgtac gcaggcggca
      781 ggtattccgg cgatcaggcg gggcggagga gcatcgcgtc gtacacgggg gattcggggg
      841 catgcttgat gcgtcccatg atctggtagc cccagcggag atacgcaccg tgcgccggct
      901 cgttgtcgat gatgcaggtg agggcggtcc agggctggtc gcggcggccg gcgatgacgg
      961 cgtcgtggag gcggcggcca acaccgtggt tggtgtgggc ggggcggacc atgagttcac
     1021 ggagccagaa gatcttttcg gccttggcgg cggcttgtgc gtcggctggc cgagcgtcgc
     1081 cgagggaggt ccaccagggc ttgttggcgg gcagggtcac gccgtggaca tagccgatca
     1141 gctgcccgtt gaggcgtgcc gtgacggttt cgaagcccgt catggtgaac gcggcatgca
     1201 gccggtccac gtaggagggg actgagaaga acggatcgtc cgcgtacggc ggaacgttgt
     1261 agatctcggc gtacgtgtcg attagttcgt cggcgatcgc cttggccgcg tccccgtcgt
     1321 agtgctggag agcgaggtcg atatccgcca cggtgttggg ttcccttcgt cgggtgctta
     1381 cgcggtgtat gcggtgaggc gctcggccat ctcgcgggcg ggctggtggg ccttgtgcag
     1441 gctcaggccg ttgcggacga gggtgagctg cttgatgttg cgggtggagg tcataccgcc
     1501 ttcgatcagc tgcagggact cgtcgaccgc gtggacgctg tggtcgaggt cgtggccttg
     1561 gagggcggcg taggcgaggc gggccaggca catgccgcgg ttgcgggagc gtgcgatgtc
     1621 ctggagttgc gccgagcggt ccaggagttt gacggactgc cggtgctgtc cgaggtcgag
     1681 gcggcacatg ccttccagac aggtgagttc ggcctcgttg aggaagccgg tccagtcggg
     1741 atcgcgctgg ctggtgccgt gttcgaagtg gttccaggcc cgcttgatgg cgttgcccgc
     1801 ggagatctcg tcgccgaggc gagcgtgccc ctgggcctct cggatggcca gcagtgccgc
     1861 gacgcggggc ggcgcggacc actcggtggc gtgggcctgg gcgatgcggg agaagcggat
     1921 cgcttcgcgg gctttgccga ggtcgacgga ctggcgggcc atgttggaca gggtgcgggt
     1981 ggcgagggcg tcgtcgccgt cgagcatcgc ggtgttgagg gcttcggaga agaagacgcg
     2041 ggcctcggtc tgccggtcgg cgtcgtagca gaaccacccg agcgaggtcg tcaactggcc
     2101 ggcgatcgca tggagttcct ggccgacggg ttcgctgtag gtgccgtggt cgatgaggtg
     2161 gtggagccac aggagctggt tgcgggcgcc acgccacagc cggtcgctgc cgaactgctg
     2221 gtcgagggca tcgaggtcgt gtgcggcgga gcggaggttt tccacgtcgt cgtggctgac
     2281 ccgcagctcg tcatcgcctg gtgctgccga ggcggtgggg gagagccagg gcaggccggt
     2341 gccggctgcg gagccgagcg cggcggctcc ggtggcgaag agttggcggc ggttcactgg
     2401 atcgtgggtc tcgaattccg tgcggatgac aggcgggacc ggctcggtga cggcgagagt
     2461 gctcggggtg gtgtctccgg tcgggctgaa gccgagctcg tggatcggtc tgtcgtaaaa
     2521 gcgttctaag agtttgcggt ggtcggggtg gggccacgga ggagcatcgc tctcccagcg
     2581 gcggaactgc cgggggctga cggacgtgcg cttcgtcagt tcgccagtct cgtacatcgc
     2641 cgcggcgagc ttggtgagct tctcggcgtt ctgctcctgt gtcaggtgca tgcgtcggcg
     2701 ctcccgcttg aggtggcggt taccgcgtgc tgcctcgctc atacgtcccc cggcgggaat
     2761 cctcacgtgt gttcacccac cgtagtggcg gcctgcatcc gatgggagcc gtcggcctgg
     2821 tcaaaaggcc gtggccggtg agaaggccgc caaaaggccg atcgagggcc ggttcctgga
     2881 cggcgagggg ccgttggaag acctgggcac cggttcagcg tggcggtgtc cttgaagcac
     2941 cggccgcgct ccgtcgcggt cgcccccatg ccgacgaagg acccccgcat gcccgtgttc
     3001 gtgatcctgc gcccgaaggc gccgagatga gcgtcaccgt gacccgatgc cccgagccga
     3061 ccgatgctca cccgcgaccg gcacagccgg tgtcttctcg tgagacgacg ctggaaggca
     3121 ccgcgaagat ggccggatgt gcccgtcggt tcctgcggcg caccgcgacg gcctggtccg
     3181 tggatgtgga cgccgtcgag gcggccgagg tggtcgtctc agagctgttc accaacgccg
     3241 tgcagcacgc acaggccgag cggattcgtc tgcgcctgcg ttgccagcac gcgctgctac
     3301 atgtggaggt cttcgacccg cagccgcacg gcgagctggt agccgcgcgg ccgggcccgg
     3361 acgctgagga cggccgcggc ctggagattg tcgaggctct gtgcctgcgc tggggccgcc
     3421 ggcctgatgc cgatcgctgc ggctcggtga catgggcctg cctgccggcc cccggcgccg
     3481 aaccgactca gcacgatcgt gccgccgacc cggaacagcc ctgcgaggcc ggtaacctgc
     3541 ctcggcccgt ggccatgttg cctgtgcacc ccgcacctgt gccgttcgaa ggagacctcg
     3601 tgccgccatc cccgccgccc ccgcctacgc tctacgagtc gtgggaggta ctcgctgacg
     3661 gccttcacgt ccagcgcctc gccctgcacc tgccgcagtt gcgcgagcag ctcaccgcgc
     3721 agacggcggg gaccggcagc acggttgctg ccttggtcgc ccgtcctgcc gaggtgccca
     3781 ccgcacaggc cctggcgctc atcgacgcgg ctggcttgag tgcctgggta gatctgcccg
     3841 ctgagcacgg ccggtccgat gtcgcggtca gccctttggc cagcgccgcc gaggacgtcc
     3901 acgccctgct tctgcccggc gtcaccgacg acgcgctgcg cggcgcggtg cgagtggtcg
     3961 ccgctgcctc ggcctggtgg gtcggtgcct tcgccgccat ccggcacctc ggcgtgcacc
     4021 acagcagcct cacgcccgtc aaggacaccg tcggctcgga cacactgcgc gaggcggtcc
     4081 gcgtcgtcgc actcggaacc gctcaacgcg ttctcgccca ccagttgcgg cacgacgcga
     4141 gtgaggaagc ggtccggctc gcgtactgcc gggccgttac cgaaggaatc gtcatcgagc
     4201 cgacgctgcc ggcgctcctg gaggagctgg gcgagctgcg gctcgtggac ctggtgacca
     4261 cggcgatccc ctggcgcggc cggttcgcga agtacgccgg aggtaccggc gccgggcagg
     4321 tggagtaaac cgatgcagaa cacgcgcaac ccgttcatcg tcgtcgaggg cctggacggc
     4381 accggcaaga ccaccttgcg cagggggctc ttccgcctct tcgagggcct gtaccgggtc
     4441 accccgctcg cggtgctgac caccaacttc ctcgacgcat cggtggccgc cgacctggtc
     4501 gagggcaagt accagccgtc cgccgacaac cagtaccgct atctggccgc gctcgccgcg
     4561 gacaagcagg ccaccgccac ccgactgatc gcgccgtcgc tgctggcgcg cccggtgatc
     4621 gcggaccggt ggctgctgtc cgaactcgcc ttcttcgcgg tcaagcacga ccggagcccg
     4681 aaggagacct atccccacct cgccgaacac ctcgacgtca tccccgacct cacgctggtc
     4741 ctcgacatct cacccgaggc cgccatggag cgggccgccg cccgctcggg cgacgccacc
     4801 cgcccggact gggacgtcct ggacatccag gcccgcgtcc gggaggtcta ccagacggtc
     4861 accgccgaac ccgccgccta cccggccctg ggcccggtgg tccgcatcga cgccgcccag
     4921 gatcgcgcca ccgtcctgca caccgcctgg caggccctcg accagcgtgg cctgctcccc
     4981 aaattgcccg aaggagccca gtgagcacgc acatggacgt ggatatatcc gcgctgaagg
     5041 accgcctcgc ccaggcccga ttagcccgca cacccctgcg cgccgtcggc gccgcgagcg
     5101 cggtggcggc caaggtcgcc gtggaagccg ggttcgatgc cctgtgggtg tcgggcctgg
     5161 aggtctccac ggccctgggc ctgccggacg agaatgtact cggcccccgc gacctgaccg
     5221 acgtcgtacg ggccctcacc cgcaccgcgc ccctgccggt catcgtcgac gtcgacaacg
     5281 ccggcggcca ggtcgccacc gcccgacgct tcgcggacga cctcgcccac gccggggccg
     5341 cagccctgtg cctggaggac agcgcctatc ccaagaccaa ctcgttcgcc ctgcaccgca
     5401 cccaggagct cgccaaaccc gtgctgatgc aggaacagtt ggcggtgatg cgggccgcag
     5461 ccgggcacga gatgctgctc atcgcgcgga ccgaggccct gatctgcggc accgatctgc
     5521 cggaggccat cagccgggcc cgcgcatacg agcaggccgg agcggacgcc gtcctgatcc
     5581 actccaagga caccaccggc cgccaggccc tggccaccgc cgccgcctgg acgggcgccg
     5641 tgcctctggt gaccgtgccc accgccttcc cgcacctggc accccagagg ctcggcgagg
     5701 ccggcttcgc gctggccatc tacgccaacc acctctcccg cgccgcgctc gccgcgatgc
     5761 gccaggtcgc agcctccttc tccgccaccg gctccttcac cgccgacggc atcccgctca
     5821 gcgacgtcgc cgacctgcta caggtcgccg acccggctgc ccgcgcctgc ctgtagatgc
     5881 caccgggccc tgctttacgg acttcgcccc gctccaaccc cgcaccacta gcatccgccg
     5941 ggcatcggcc gcggcgggct cgacgaggga aggaacacac cgcgccatga cagccctgcc
     6001 ccgcatcagc gtgtcggtca aggccgcgat cgtccatcaa gaccgcatcc tgctcatgtc
     6061 gtacgacgac cactccggct tccactacaa cctgcccgga ggcaaggcga aggccggcga
     6121 gccgctgcgc gacgccgtgg tgcgcaaggt caaggacgag accgggctcc gggtgcgcac
     6181 ctcacggctg ctgttcgtcg tcgagtacgt ccccgagcag tacaacgacg aattcggcac
     6241 cgtccacaag acccagcaca acttcctcgc cgagctcctc gacgaggaca ccgcgccgcg
     6301 caagtccgac ccgccggacc cgatccaggt cggcttcgaa tgggtgccgc tcgaggagtt
     6361 cgaggacaag tacctgctgc cgcgggtcaa ccagcaactg ctcaacgcgc ttgccgggga
     6421 cccggccgac cgcgacgtcc tcgtggaccg gtggtgacca tgcactcccg ccccgcttcc
     6481 gtgctgaacc tcgccgaact gcgcgactgg ctcctcgatc accacggcaa caccgtggac
     6541 gcggaccggc tgcccgccat ccgcaccgaa ctggaacacc tcaccacccg tcaggttgcc
     6601 ggagccgtcg tggaactcgg ctgctaccag ggagctatgg ccttgtggat gcgggccgtg
     6661 ctggacgcca ctgcacagca cgaacgcccc gtccacgtct acgactcctt ccagggactg
     6721 cccgaacgca gcgaacagga cccggagctg ttcccggccg gattcctgcg cgccgagccg
     6781 gagcaggtgc tcgccctgca cgaccgctgg ggccgcaccc cgcccgtgat ccaccccggc
     6841 tggttcgccg acaccctccc cgaccagctg cccgaccgga ttgccttcgc ctacctggac
     6901 ggcgactact acgactccat ccgcacctgc ctgaccgcct gcgttccccg aatggcccca
     6961 ggcggcacgc tgatcgtcga cgactacgcc gacctggagg cgaatccgaa ggcgtggaac
     7021 ggactgcccg gcgtcaaagc ggcctgcgag gactactttt gcacgccgtc tccgctaaag
     7081 gtcatcgtcg gcgccggcga cctggccttc ggccgctaca ccgcgcccgc gagggcagag
     7141 cagtgagcgg catctcggcg gccccgctgc tcgtgatggt gcccggcgcg gccgaccccg
     7201 tggtgctcag gcggcacctt cccggctgcg cgatccgcgt gattgagtcc ctcgacacgg
     7261 ccgccctgaa tctggccgcc cgcgccgagg tacttgtgct caggtcgggg caccggctcg
     7321 acgccgagcg cctgacccgg tggccgaaac tgcggcatgt gatccgcgcc gggtcggggc
     7381 tggacggcct cgacgtcgag gccctggccg cacgcggcgt caccgtgcac cgcaacccgg
     7441 ccccggccgc cgacgcggtc gccgaatggg cgctggccgc attactcggc ctcgctcgcc
     7501 ggattccgta cgggcacgcc cccctgctca gggggcagca cacgaagcag cagtgcatcg
     7561 ccccgccgat ctcccgtatg agggtggcga tttggggtgc cggccctgtt ggccgggcat
     7621 gcgcgaccgc gctcgcgccg tacgtcgacg agatcgcctt cgcggcccgg cccagcccga
     7681 actcaccgag ttcctggtgg acgtgctggg ggtgacggac gtcggggcgt actacccgca
     7741 ccgggtgacc taccacccga cctgtcacgg tctgcgctcc ctcgggctgg gcgagcggcc
     7801 gctgcggctg ctgcgcgcgg tgaagggact ggagctgctg gagctgccgg gcgcggacga
     7861 gtgctgcggg ttcggcggga cgttcgcgct gaagaacgcc gacgtgtcgg cggccatggg
     7921 cgcggacaag gtgcgcaacg ccgaggcgac gggcgccgag gtgctgtgcg cggcggacaa
     7981 ctcctgtctg atgcacatcg gcggcacgat gacgcggctg cggaccggca tgcggccggt
     8041 gcacatcgcg gagatcctgg cgagcacgga ggaggacccg gccgtatgag cggaacgttc
     8101 gtcggtatgc ccgctttccc ggcggcggcc cgggacgccg tgcgggacac gaccctgcgc
     8161 ggcaatctgc ggcacgccac gcacaccatc cgcgccaaga gggcgcgggc ggtcgcggag
     8221 ctggacgact gggagcggct gcggcaggcg ggtaagcgga tcaaggaccg cacgctccgt
     8281 catctcgacc gttatctggt gcagttggag gagtcggtga cggcggcggg cggtgtggtc
     8341 cactgggccg ccgacgccga cgaggcgaac cggatcgtct ccgaactggt gaaggcgacc
     8401 ggcgagacgg aggtcgtcaa ggtcaagtcg atggccacgc aggagatcgc cctgaacgag
     8461 gcgctggagg cgcagggcat ccacgcgtac gagaccgatc tcgccgagct gatcgtgcag
     8521 ttgggcaagg accggccgtc gcacatcctg gtcccggcga tccaccgcaa ccgcggggag
     8581 atccgggaga tcttcgcgcg ggagatgggc gagtgggggc gccccgcgcc cgaggggctc
     8641 accgacacgc ccgccgacct cgccgaggcg gcgcggctgc atctgcggga gaagttcctg
     8701 cgcgccaagg tcggcatctc cggcgccaac ttcatggtcg ccgagaccgg cacgctcgtc
     8761 gtggtggagt ccgagggcaa cgggcggatg tgcctcaccc tgcccgagac gctgatctcg
     8821 gtggtgggca tcgagaagat cgtgccgacc tggcaggacc tggaggtgtt cctgcagacc
     8881 ctcccccgct cctccaccgc cgagcggatg aacccgtaca cctcgatgtg gaccgggacc
     8941 acggacgcgg acggtccgcg cgccttccat ctggtactgc tggacaacgg ccgcaccgac
     9001 accctcgccg acgaggtggg ccggcaggcg ctgcgctgca tccgctgctc cgcctgtctg
     9061 aacgtctgcc cggtgtacga gcgggccggc gggcacgcgt acggctcggt ctatcccggc
     9121 ccgatcggcg ccatcctcag ccctcaactg cggggcaccg gcagcgagat cgacgcctcg
     9181 ctgccgtacg cgtcctcgct gtgcggggcc tgctacgagg tgtgcccggt cgccatcgac
     9241 atccccgagg tgctggtgcg tctgcgggag cgggtcgtgg agggcggcga ggccgtccgc
     9301 gacggcaaca aggtgcggct gcggcccgcg aagggccacg cggccgaacg ggcggcgatg
     9361 cgtgcggccc gctgggcgct cacccgtccc ggcgccctgc gcaccgggca gcggctcgcc
     9421 tcgcacacgc ggcggctgca tccgcgcacc ctgccggggc cgggcaaggc gtggagcggg
     9481 acccgggacc tgccgccggt gccggccgag ccgttccggg actggtggcg gcggacgcgc
     9541 ggcgggaaag acggtggagc gtgagcagca gggagcggat tctgggccgg gtgcggcggg
     9601 ccctggcgga cgtgccgccg gacgaggacg cgccggtcga gcgggactac ctgcgcgagc
     9661 acggggagcg gaccgtcgag gagacggtgg acctgctggc ggagaacctg ggggactacc
     9721 gggcggtcgt gcaccgcgcg gactcggagg aactgcctta cctgatcatg cggttgctcg
     9781 cccagcgggg accgcagtac gtgctcgtgc cgccgggcct gccgccggag tggatgtcgg
     9841 ccgcggatcc gacgcgcgtg cacgaccgcg cggtcagcac cgcgcgggag ctggaccagg
     9901 tggagagcgt ggtgacgggg tgcgccgtcg ccgtcgccga gaccggcacc atcgtgctgg
     9961 acggctcccc cgaccagggc agacgccgga tcagcctgat ccccgaccac cacatctgcg
    10021 tggtgcgcgt ccccgaccag gtggtggcgt ccgtccccca ggccctcgaa cgcctggacc
    10081 ccgcccgccc gttgacgtgg atctccggac cctccgcgac cagcgacatc gagctggacc
    10141 gggtcgaggg cgtgcacggg ccgcgcaccc tggaggtcgt gctgctccgg tgaaccaccg
    10201 tccgggggtg tcgctgcgta cacccccacg acgggcactg cgccccgtgt ggccgcccgt
    10261 ctcctccctg gcgtcgtgac cagggcacag ggcgtgccgg acggggagat gagggtggtg
    10321 cgcgacagga cgcggcacga ccggcgcacg gcactcggcc gcctcaccgg aggcgctgca
    10381 cgcggcggcg accgccggca cgagcctgtc cacggcgcgg cgcgtgctgc gcgccccggc
    10441 ggtgcggacg gtgggcacga gcgcgggacg gagcgagcgc gtgacagggc gaccgcaggg
    10501 cggagccgcc gcgtgaagcg accgcagtgc ggaggtgagc gcgtggcagg gcgagcgcgg
    10561 gacggagcga gcgcgtgaca gagggatgag caagcgctta gataggtgtc cgggacccca
    10621 cagggatgcc cggacaccgt cgttcccgcg cccagccgga ggccgcagtt gttcacttcc
    10681 gtcgacgacg tctccactcg cctcgccgag accggctacc tggcctcgcc cgcggtcgcc
    10741 accaccgtct tcctcgccgc ccgcctcggc aagccgctgc tggtggaggg ccccgcgggc
    10801 gtgggcaaga ccgagctggc caaggccgtc gcccgcgcct gcgacgcccg gctggtgcgg
    10861 ctgcagtgct acgagg
//
