LOCUS       NZ_RAII01000001        22494 bp    DNA     linear   CON 15-MAY-2024
DEFINITION  Streptomyces sp. E1N211 s-211_S3_L001_R1_001, whole genome shotgun
            sequence.
ACCESSION   NZ_RAII01000001
VERSION     NZ_RAII01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. E1N211
  ORGANISM  Streptomyces sp. E1N211
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7318313)
  AUTHORS   Vicente,C.M., Thibessard,A., Lorenzi,J.N., Benhadj,M., Hotel,L.,
            Gacemi-Kirane,D., Lespinet,O., Leblond,P. and Aigle,B.
  TITLE     Comparative Genomics among Closely Related Streptomyces Strains
            Revealed Specialized Metabolite Biosynthetic Gene Cluster Diversity
  JOURNAL   Antibiotics (Basel) 7 (4), 86 (2018)
   PUBMED   30279346
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7318313)
  AUTHORS   Leblond,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2018) Dynamique des Genomes et Adaptation
            Microbienne UMR 1128, Universite de Lorraine - Institut National de
            la Recherche Agronomique, Faculte des Sciences et Technologies
            Boulevard des Aiguillettes BP 70239, Vandoeuvre-les-Nancy F-54506,
            France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics Workbench v. 6.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003671735.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 02:33:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,941
            CDSs (total)                      :: 6,866
            Genes (coding)                    :: 6,131
            CDSs (with protein)               :: 6,131
            Genes (RNA)                       :: 75
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 736
            CDSs (without protein)            :: 735
            Pseudo Genes (ambiguous residues) :: 0 of 736
            Pseudo Genes (frameshifted)       :: 138 of 736
            Pseudo Genes (incomplete)         :: 673 of 736
            Pseudo Genes (internal stop)      :: 17 of 736
            Pseudo Genes (multiple problems)  :: 87 of 736
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:49:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2964711
            Orig. end                         :: 2987205
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            RAII01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..22494
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lassopeptide"
                     /region_number="12"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..22494
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22494
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12494](-)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..12494)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     misc_feature    511..526
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.55"
                     /tool="antismash"
     misc_feature    complement(562..572)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     gene            1380..2069
                     /locus_tag="EBH12_RS13815"
     CDS             1380..2069
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010351849.1"
                     /locus_tag="EBH12_RS13815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /protein_id="WP_114874608.1"
                     /transl_table=11
                     /translation="MTTAPGTVLVVEDEESIADVLAIALRYHRFEVMTAGTVREALALT
                     EHTRPDCALLDVMLPDGDGRALGRELRGRRPETALVFLTARDAPAEIVGALGFGDDYIT
                     KPFNIDEVVARITAVLRRTRPADVLPQRAPLRHGDLELDETTYSVRRAGRSVELTPTEY
                     ALLRFLVRNAGRVVPKEQLLRHVWQYEHVPQESTVVETYISYLRRKLDTLGPPVITTRR
                     GVGYGLT"
     gene            2066..3538
                     /locus_tag="EBH12_RS13820"
     CDS             2066..3538
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133461.1"
                     /locus_tag="EBH12_RS13820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="WP_114874607.1"
                     /transl_table=11
                     /translation="MTRRIRLPHPGRPRVRSLRAQLTLANVVLLALGIVAATAVSVLGM
                     RYYLLDQIDTELVRTRDSLGGSELTLRQIDSLSALRFLRDRVDPEWNQDDDAPDSVFAA
                     VDREGEPVPVFGFAPSRAQHDLADAVGDPGALIRDPEPQDLTVRGNAYRVTAARLADGT
                     GVLIAGSTEPLHDGVAKAVRFDLAIGTLLLLVLACLTMMAVRRRMRPLEDMVETSSAIA
                     EGDLTRRVPSSCDTSLEVEQLRLALNSMLQQVESAYRTRERSAAQLRRFVADASHELRT
                     PLAAIRGYLQLYERGMLRDPTERRRAWDRMTTESDRMGRLVDELLVLARLDQRPELRKR
                     NVDVCRLARDAAQDLRVQQPDRPVRVDAEGAVLVHADEAGLRQILGNLVGNVRTHTPAD
                     VPVRLGVARVDGEVRLWVADEGPGLAPEDAGRVFDRFFRAGGGAGSGLGMAIVQGVVHA
                     HGGEVTVDTAPGKGLTVTVTLPPCTETFTRAD"
     gene            complement(4861..5538)
                     /locus_tag="EBH12_RS13830"
     CDS             complement(4861..5538)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020123207.1"
                     /locus_tag="EBH12_RS13830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /protein_id="WP_114873953.1"
                     /transl_table=11
                     /translation="MAEAVRVLIADDQALLRGSFRVLVDSTPGMQTVGEAGDGAEAVAL
                     ARELTPDVILMDLRMPVMDGIAATREICSDASMAGVRVLALTMFDMDEYVYPALQAGAS
                     GFMLKDASPEDLVAGIRVIATGEGVLAPSVTRRVIANFSHRDTAARPAPRLVGLTRREQ
                     EVLVLIAKGLSNAEIADRLVISLPTVKTHVSSLLSKLNARDRAQLVIVAYESGIAERGM
                     PPA"
     misc_feature    5523..5537
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.46"
                     /tool="antismash"
     gene            complement(5638..6960)
                     /gene="mslH"
                     /locus_tag="EBH12_RS13835"
     CDS             complement(5638..6960)
                     /codon_start=1
                     /gene="mslH"
                     /inference="COORDINATES: protein motif:HMM:NF038314.1"
                     /locus_tag="EBH12_RS13835"
                     /note="Involved in the biosynthesis of MS-271 and related
                     lasso peptides; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /product="lasso peptide C-terminal Trp epimerase"
                     /protein_id="WP_114873954.1"
                     /transl_table=11
                     /translation="MSRLTVALAGDCMMTRGAPLTSDPAAGRLGEVLHTADFAFTNLEV
                     APATGRGHPVPDAASGGGLIADPAVLDDVTGMGFSALSCANNHALDLGTEGVLGTVDLL
                     RAKGVPFAGIGADLTAARRPVYVDRPAGSLALIACTATFRDGQEAAAPSPDLPGRPGLS
                     PLRHGVTLRVTAAQLEALREIDGATGLRSRRAEARTLLGFDPAMPGPGRLALFGTRFET
                     ADTPGLTTACDPRDVHDITRWVAEARRRADLVLVSVHSHEPGPTPETPAEFLRDFARAV
                     IDAGADAVVGHGPHMLRGVELHRGRPVFHSLGNIVSQIELADRIPAEDYARAGTDEALT
                     PGGYFERITAHGRRMFAPHRRYWQSLVPVLTFDDGVFTGARLHPVDLGFGRPVHRRGRP
                     RLAGPVDAKEILTDFARLAEPHGVTVTVGDDGTGELVAAET"
     gene            complement(6957..7682)
                     /locus_tag="EBH12_RS13840"
     CDS             complement(6957..7682)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /locus_tag="EBH12_RS13840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="thioredoxin domain-containing protein"
                     /protein_id="WP_048459504.1"
                     /transl_table=11
                     /translation="MQPSTDAQVREMVHRRRRRRRTVLVSLAAVVTVLGAALVGAGLVR
                     ATDTEPGDAPEEVPAGVAADRAGLVTSEGPVRVDLYLDYLCPECRITEKALAPELREMQ
                     ERGEVRVVHHPVAFLDDRSAPAGYSTRAASAAACAADRNKFEPYTAALFDEQPPEQGPG
                     LDTDRLVALGRDVGITGASFERCVRDGTYRPWVTYVSDVAASRGVALTPTVKVAGKRVD
                     LSGPDRAKAFVRAVEESRS"
     gene            complement(7685..8158)
                     /locus_tag="EBH12_RS13845"
     CDS             complement(7685..8158)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /locus_tag="EBH12_RS13845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="WP_206614275.1"
                     /transl_table=11
                     /translation="MTFVRTWAGTAARIVLAAVLGYAGWVKVQDLTGAGRTVALYQLVP
                     EESAQLVGAAVVFVELALALLLLAGLATRAVAAVTALLMVTYIAAIVSVWARGMSIDCG
                     CFSSGGTLTGGAERGYVVDIARDLAFLGAAAFLITRPRTRYALDRLVLETKER"
     gene            complement(8173..9042)
                     /locus_tag="EBH12_RS13850"
     CDS             complement(8173..9042)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1065: ABC-2 type
                     transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020123205.1"
                     /locus_tag="EBH12_RS13850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter permease"
                     /protein_id="WP_086700783.1"
                     /transl_table=11
                     /translation="MTRTDIPPSVTVTKPAPPSSEGRTWHTVRPYALLWRREMTRLRHN
                     PLRLVMGLVTPLLFLVVLGTGLEAASSTLGKAQLNDYRAYLFPGALVMSVQAPAIAVGI
                     SLVWDRRLGMLRQMLVSPFPRSSIVLGLALGGATTGAVYGLALLAVGGIAGVRYTPMLL
                     VVLVELLLVSLLFTSFGLLAAVTIRQVDTFQIVVNLSLMPLMFFSGAMFPPNGLPGWLD
                     TVVKLNPLTYGVDAVRRTLPGPDVLTSEQTRLMLGDWNPPVYAELGVLAALTAAVLGLA
                     TYRFSRAQ"
     gene            complement(9039..10004)
                     /locus_tag="EBH12_RS13855"
     CDS             complement(9039..10004)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007385816.1"
                     /locus_tag="EBH12_RS13855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_114873957.1"
                     /transl_table=11
                     /translation="MTAIRAEGLYAYYGTTPAVNGLHLNVPEGATFGFLGPNGAGKTTT
                     ISMLTTLLRPTAGRAEVAGFDVTTHAAEVRRRIGIVFQESTLDLELTATENLRFQADLC
                     GLGRAEARAAVAAMLDMMELTGRDRTPVRHFSTGLRRRLEIARGLLGSPRVLFLDEPTT
                     GLDTQTRAAVWHHLDRLREERGITVFFTTHQLEEAEHCDRIAIFDRGKLVTEGSPAELK
                     SVIGADVVDLRTDDDRLAVDLLSDRFGLTAENTPGGLRLRVQDGASMVPRLCTGLGLGV
                     RSVTVTPPSLDDVYLHHTGTAIRDSGSDARSLDSLGEGLR"
     gene            complement(10001..10426)
                     /locus_tag="EBH12_RS13860"
     CDS             complement(10001..10426)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020123203.1"
                     /locus_tag="EBH12_RS13860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_048459500.1"
                     /sec_met_domain="PF13471 (E-value: 4.5e-25, bitscore: 86.6,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTPAVAEQATRLPLHRQIAPRCAAGAARLLVRLPPARLHRVLRV
                     LSKGSRPAGYAQVARARRSVVSVSTRCAGLGCLQRSVATVLLLRVRGRWADWCTGFRVQ
                     PFAAHAWVEAGGRPVDEPGEVGVFRTVLAVRRTGGGS"
     gene            complement(10423..10686)
                     /locus_tag="EBH12_RS13865"
     CDS             complement(10423..10686)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lasso_Fused_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF033530.1"
                     /locus_tag="EBH12_RS13865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_048459499.1"
                     /sec_met_domain="Lasso_Fused_RRE (E-value: 5.1e-07,
                     bitscore: 28.3, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 9.8e-22,
                     bitscore: 75.4, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 8e-11, bitscore: 40.7,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLSLARDVTLTPVDSGAVLLDGRRGRYYQLNASGSAILHRLLDG
                     DTPAAAAVSLSESAPVSEERVHQDVLALVRSLSEADLVEVTQ"
     aSDomain        complement(10432..10680)
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_EBH12_RS13865_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="4.00E-24"
                     /identifier="RREFam002.1"
                     /label="EBH12_RS13865"
                     /locus_tag="EBH12_RS13865"
                     /protein_end="85"
                     /protein_start="2"
                     /score="75.4"
                     /tool="antismash"
                     /translation="LSLARDVTLTPVDSGAVLLDGRRGRYYQLNASGSAILHRLLDGDT
                     PAAAAVSLSESAPVSEERVHQDVLALVRSLSEADLVEV"
     aSDomain        complement(10438..10686)
                     /aSDomain="Lasso_Fused_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing fused peptidase in a lasso
                     peptide cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_EBH12_RS13865_Lasso_Fused_RRE.1"
                     /evalue="2.10E-09"
                     /identifier="RREFam003.1"
                     /label="EBH12_RS13865"
                     /locus_tag="EBH12_RS13865"
                     /protein_end="83"
                     /protein_start="0"
                     /score="28.3"
                     /tool="antismash"
                     /translation="MKLSLARDVTLTPVDSGAVLLDGRRGRYYQLNASGSAILHRLLDG
                     DTPAAAAVSLSESAPVSEERVHQDVLALVRSLSEADLV"
     gene            complement(10683..12494)
                     /locus_tag="EBH12_RS13870"
     CDS             complement(10683..12494)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_067372303.1"
                     /locus_tag="EBH12_RS13870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide isopeptide bond-forming cyclase"
                     /protein_id="WP_114873958.1"
                     /sec_met_domain="Asn_synthase (E-value: 1.5e-37, bitscore:
                     128.8, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFTVLPDCPAGAALADRVAAPKRIDHASGRPWIVGDWPEGGTTV
                     TEAGTRRLAVFGHTRPDDAGTASALGRMRSLHDVDRVASRLPGVFHLAASLDGAVRLQG
                     SVAGVRQVFTARVDGVTVAASAVDPLLRLTGAGLDETLLAARLLAPGGAPWPLSPRPVR
                     RGVDALPTGHWLELGADGRARSVRWWELPEAALSLEEGAGAVRSALTDALATRVDPHRT
                     ISADLSGGLDSTTLCFLADAAGADLVTYHVMPLDEANEDTAWARKAAAHLPHARHHMLA
                     ADRAANLFDIGYTADTVNAAPEGPSTWASGLAHIRDLAGRATAEGAALHLSGFGGDELF
                     GRMPACAWSLARLRPADGLRLVNRYRLANRWPWRSTLRQLADRSTFAQNLTEVAGRITA
                     PPPPVNEPDFGWVFAPRMPAWATPDAVAAVRDLLTTAAERTPEPLDADRARHQALSSIV
                     FEGNTVRQVNTAVAGTGLVWEAPFLDDRVLEAAFATRIDERLAAGRFKPLLTTAVRGLV
                     PDDFLARRDKGEFSAETFRGIERNRDRILDLCEDSLLARLGLVDPHAFRSAVLNPGPMS
                     HHLQPIQTTVACESWLRAHPQDTGENR"
     gene            complement(12556..12684)
                     /locus_tag="EBH12_RS13875"
     CDS             complement(12556..12684)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_103079659.1"
                     /locus_tag="EBH12_RS13875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aborycin family tricyclic lasso peptide"
                     /protein_id="WP_106963308.1"
                     /transl_table=11
                     /translation="MTAIYEPPALQEIGDFDELTKCLGIGSCNDFAGCGYAVVCFW"
     gene            complement(12801..13553)
                     /locus_tag="EBH12_RS13880"
     CDS             complement(12801..13553)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /locus_tag="EBH12_RS13880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter permease"
                     /protein_id="WP_048459497.1"
                     /transl_table=11
                     /translation="MSETLAAARAEFTKMRAVRATSVALLLFVAVSVFIAALGGWSAKG
                     AIESGNPGLRSDFTPEQAGLDGILYGQLALIVFGVLMMSGEYTSGMMRVSLLAVPRRGR
                     LYLAKTAVTALAALAVALPVTVVSYLVTQLALGPHGSTLDADGVPRALAGAVVYLTLMS
                     LLAVGVAAAARSAVLPLAVLLPLVLVGSQILSVIGATKEVARWFPDRAGAQMLTVDSGD
                     ALTGLVVLLAWTAAALTAGWLRHRSWDG"
     gene            complement(13546..14448)
                     /locus_tag="EBH12_RS13885"
     CDS             complement(13546..14448)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006136293.1"
                     /locus_tag="EBH12_RS13885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_114873959.1"
                     /transl_table=11
                     /translation="MLAERLTKRYGPATVVDDLSFTVRPGVVTGFLGPNGAGKSTTMRM
                     MLGLTRPDGGTATVGGRAYRDLTYPLRHVGALLETSAPHRGMTAVGHLSWLARSNRVPR
                     RRVDEVLDAVGLTQAARKRVGTFSLGMGQRLGLAAALLGDPPVLVLDEPVNGLDAEGIR
                     WLRELLRAKAAEGRTVLVSSHLMAEMAQVADELIVISRGRLLAETSVSEFLGRHGRTFV
                     RVRTSEPLRAAQEFQAKGATSVRRAADGGLEVDGLPAGEVNRIAAAAGVPVDELSTHTG
                     SLEETFLKLVDDGGDPTHV"
     gene            complement(14559..15698)
                     /locus_tag="EBH12_RS13890"
     CDS             complement(14559..15698)
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018958865.1"
                     /locus_tag="EBH12_RS13890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sensor histidine kinase"
                     /protein_id="WP_114873960.1"
                     /transl_table=11
                     /translation="MGEGSRRRAIVLDAAAACAFTLLPQVSLLRPAVDGGTGGGLLWLL
                     SLAAGLPLAVRRLWPVPVFVLVLAAACWALAAGLGPASFLAAAYAVHTVATTRRRDPGV
                     SAVAVAGLCAAVAALLTLSGGQSYQGGSTAVQAVFGLLVLGATWAAGSAVRERRRSTRR
                     AIEHAAEQAKTEERLRIARDIHDVVTHSVGLIAVKAGIANHVVATRPEEAKEALAVIED
                     VSRRALRDMRATLTVLRGEDRSEAGDLRPARGLADLPALLETAEAAGVRVELRTRYDQE
                     PPEGVALAAFRIVQESLTNVLKHAAPTGCRVDVTARQDVLTVDVTDDGPGPGRRTTVPG
                     GGMGLAGMKERAAAHGGTLAAGPRPGGGFRVTATLPFQG"
     misc_feature    15920..15936
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.23"
                     /tool="antismash"
     gene            16114..16884
                     /locus_tag="EBH12_RS13895"
     CDS             16114..16884
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /locus_tag="EBH12_RS13895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /protein_id="WP_155553636.1"
                     /transl_table=11
                     /translation="MCPRGPSGAVRTTPTHFWGMYEMTVNLFVVSDNAVSLAGLEAIVD
                     QDEECALVGRATDDSDDLRRLSDLAGTALHPDVVVLSEPSSSRTVSARVRHIASAFPDE
                     ASRPHTIVVAQNDDDEMIVSALRAGVNGYLSRLGSADELLRSLKVVARGGAAFCPTVAA
                     RMSRYFSAMQGLHDGAGFPGLTSRELEILELLAGGMGNRQIARRLFLAEKTVRNYVSRI
                     FAKLEVHDRATAVVMARDAGFGEPAGELVPHGAL"
     gene            16947..17198
                     /locus_tag="EBH12_RS13900"
     CDS             16947..17198
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018845686.1"
                     /locus_tag="EBH12_RS13900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_114873962.1"
                     /transl_table=11
                     /translation="MSGSKKYSISLPEDLAEAVRTHVGPGGFSAYVAEALEQRVAMDKL
                     HEIVADFETDNDPLSREEIEAARAVLRHDRHDSSGAAA"
     gene            17198..17590
                     /locus_tag="EBH12_RS13905"
     CDS             17198..17590
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007451887.1"
                     /locus_tag="EBH12_RS13905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DNA-binding protein"
                     /protein_id="WP_114873963.1"
                     /transl_table=11
                     /translation="MPGTLILDSEGLSKLYLKDRTVLAFIQAATEEGTRVATSAMTTLE
                     ADYERVHPARIRWVLSRIDVHDVTEAVTGQASALLRDHRLSGHKYAIDAVLAAVARSAP
                     GPVTVLTSDPEDIGLLCGSAIEVIKA"
     gene            complement(17734..17916)
                     /locus_tag="EBH12_RS13910"
     CDS             complement(17734..17916)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EBH12_RS13910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_114873964.1"
                     /transl_table=11
                     /translation="MADTDAGVGMGFTWAVNTAQCRPTPPAALLIGAISDGIGPPAEPA
                     WGALKGRFAGKPDGL"
     gene            18003..18257
                     /locus_tag="EBH12_RS13915"
     CDS             18003..18257
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018568596.1"
                     /locus_tag="EBH12_RS13915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="WP_045563117.1"
                     /transl_table=11
                     /translation="MARTVIDLDEDMVTEAMRIFGTKTKAKAVRLAMEDAVKRHLRQEG
                     FDAMDAGELDFSEIVENTGPRNADGSLKHNGDRDGGRAA"
     misc_feature    18015..18029
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 15.56"
                     /tool="antismash"
     gene            18257..18676
                     /locus_tag="EBH12_RS13920"
     CDS             18257..18676
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019355944.1"
                     /locus_tag="EBH12_RS13920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PIN domain nuclease"
                     /protein_id="WP_114873965.1"
                     /transl_table=11
                     /translation="MQDRYLIDKSALARWTKPSVKEVLKPLHERCLLAVCRPTEYEMIH
                     SARDSSEATRISTWLHAFDYLRIDDDTFTRALEIQRHALNAGFHRALSLPELLIAATAE
                     LNRRTVLHYDGDFDMIASLTGQPTEWVVPPGSADR"
     gene            18862..19815
                     /locus_tag="EBH12_RS13925"
     CDS             18862..19815
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EBH12_RS13925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_196421273.1"
                     /transl_table=11
                     /translation="MSEYQYYEFQAVDRPLTKDELERVRALSSRARISATHFVNEYDYG
                     DFRGDERKLMEQVYDAHLYFANWGSRRLMLRLPTTLLPARSAEPYCVENALTCWTRKGH
                     LLLDFSYEAGDGGEWDFETSFTLASFTTLRTELAAGDLRPLYLAWLSGLTRWELEEDVD
                     EDAYASALEPPVPTGLAHLTGPQKSLADFLHVDPHLLTAAAHASGAAPSQAIDKDELAA
                     WIRALPQHEKDALLLDAALGTAPQPGPALLARHRAASRGTAVSSTAARRRSAAELLDAA
                     HGVRTEHTRRQKQAGAQARRAEQRARESPHVADRSI"
     misc_feature    complement(20220..20240)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.42"
                     /tool="antismash"
     misc_feature    complement(20366..20382)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.81"
                     /tool="antismash"
     misc_feature    20366..20382
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 21.75"
                     /tool="antismash"
     gene            <20444..20731
                     /locus_tag="EBH12_RS13930"
                     /pseudo=""
     CDS             <20444..20731
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014669705.1"
                     /locus_tag="EBH12_RS13930"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="HWRRASDALPHHSPALGYAPRTFSRAVLQATGRTAKAYIVERIAL
                     EATRLLAHDRLTAARCADVLGFTDASNFSVFSRKATGMRPGTWQATVAAE"
     misc_feature    complement(20951..20971)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 22.62"
                     /tool="antismash"
     misc_feature    complement(20963..20976)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.12"
                     /tool="antismash"
     gene            21042..21890
                     /locus_tag="EBH12_RS13935"
     CDS             21042..21890
                     /NRPS_PKS="Domain: PKS_KR (5-160). E-value: 3.4e-09. Score:
                     28.8. Matches aSDomain:
                     nrpspksdomains_EBH12_RS13935_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828025.1"
                     /locus_tag="EBH12_RS13935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="oxidoreductase"
                     /protein_id="WP_114873966.1"
                     /transl_table=11
                     /translation="MQKQNWLITGVSSGLGRAFAQAALAAGHAVVGTVRSEKDLQAFEE
                     LRPGHAHGRILDVTDGDAVSSVVAEVERSVGSLDVVVANAGYGLEGTFEETPLAEVRRQ
                     FEVNVFGAVATLQAALPHMRARRRGHLMGVTSMGGLMAVPGMSAYCGSKFALEGILEAL
                     GKEVAQFGIHVTAIEPGSFRTDWAGRSMTRAARTIDDYDELFAPIREARQKASGNQLGN
                     PAKAGDALVHIASLERPPAHLVLGSDALRLVTAARTAVDEDIRAWEALSRTTDFADGAQ
                     L"
     aSDomain        21057..21521
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EBH12_RS13935_PKS_KR.1"
                     /evalue="3.40E-09"
                     /label="EBH12_RS13935_PKS_KR.1"
                     /locus_tag="EBH12_RS13935"
                     /protein_end="160"
                     /protein_start="5"
                     /score="28.8"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="WLITGVSSGLGRAFAQAALAAGHAVVGTVRSEKDLQAFEELRPGH
                     AHGRILDVTDGDAVSSVVAEVERSVGSLDVVVANAGYGLEGTFEETPLAEVRRQFEVNV
                     FGAVATLQAALPHMRARRRGHLMGVTSMGGLMAVPGMSAYCGSKFALEGIL"
     misc_feature    22055..22071
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 18.96"
                     /tool="antismash"
ORIGIN
        1 agcaggtcgt aggccttgcg ggtggtggtc gaggcgtcgt cgttgccctg gtccacggtg
       61 cccagacgca gcgacagcag cggcaccgcg agcgcggcca tcagcaccac cgccacggcg
      121 gccaccgcgc ggggcctgcg ctgcacggtc gccgaccagc gcgcggagag accgctcacc
      181 tgctcgccct tcgtctccga agccgcgagc cggcggcgct gacggcggct gagcacccgg
      241 tgccccagca ccccgaggag ggcgggcagc aaggtgatcg cggcgagcac gctgagcacc
      301 accgtcaggg aggtgccgat gacgacgccg tccaggaacc gcaggttggt caccagcatc
      361 ccggcgagcg cgatgcacac cgtgccgccc gcgaacagca ccgcccggcc cgaggtgttg
      421 agcgcggcga ccgcggcctc ctccgggtcc ttgccgctca ggatgccctt ccggtgccgg
      481 gtgacgatga acagggcgta gtcgatgccg acgcccagac cgatcaacgt ggcgagcagc
      541 gacgccagtt cggggacgtc ggtgacatgg ctgatcagct gcgtgccgaa catgccgccc
      601 gcgacgccga acacggcgac cacgatcggc aacagcatcg cgaacaccga cccgaacgcc
      661 aggaacagca ccacggcagc cgcgaggatg ccgaccagtt cggccgtgcc cgtcggcggc
      721 tcctggaccc gggtgacggc ctggccgccg agctccacct ggagcccgtc accggccgcc
      781 gcccgcgccg tgtcgaccac gtcctgcacc agcgccttcg gcaccgcgtc cgcctgctcg
      841 gcgaacgtca cctgcgcgta ggcgatccgc ccgtcctcgc tgatctgcgc cgcgccccgc
      901 tcgtcgtagg gcccggcgac cgcgccgacg ccctccatgc gcccgatctc cgcgagcgcg
      961 tcctccatcc gggagcgcac cgccgcgtcg cgcacggtgc cctcgtcggt cttccacacc
     1021 acggtgtccg tgtccccggc ccgctccggg aaggccttgc gcatcaggtc gtaggcccgc
     1081 ttggagtccg tgtccgggag ggagaagacc tccgcgtagt tcgtgcccgc gctgctcgcc
     1141 gtcacgccca gtccgaacag cgcccccacc cacagcacca ggaccaccag ccgatgccga
     1201 tagcaccagc gtgccaatac cgccacgtgt ccgctccttc gtcgctccgt cggtcccccg
     1261 ggtcctggcg tcagaatcgg cggcggcacc ctcccgggga cacgactcgc ccgggactct
     1321 caaggaactc caaagcgaca accggcactt cgccggacgg tcacgccgat actggggaca
     1381 tgacgacagc tcccggcacc gtgctcgtcg tggaggacga ggagagcatc gcggacgtcc
     1441 tcgcgatcgc gctgcgctac caccgcttcg aggtcatgac cgcgggcacg gtccgcgagg
     1501 cgctcgccct caccgagcac acccggcccg actgcgccct cctcgacgtg atgctgccgg
     1561 acggcgacgg ccgcgccctc ggccgcgagc tgcgcgggcg caggccggag acggcgctgg
     1621 tgttcctcac cgcgcgggac gccccggccg agatcgtggg ggcgctcggc ttcggcgacg
     1681 actacatcac caagccgttc aacatcgacg aggtggtcgc ccgcatcacg gcggtgctgc
     1741 gccgtacccg ccccgccgac gtgctgccgc agcgggcgcc gctgcgccac ggcgacctgg
     1801 agctggacga gacgacgtac tcggtgcgcc gcgcgggccg cagcgtcgag ctcaccccga
     1861 ccgagtacgc gctgctgcgc ttcctggtgc gcaacgcggg ccgggtcgtg cccaaggagc
     1921 aactgctgcg gcacgtctgg cagtacgagc acgtcccgca ggagtcgacc gtcgtggaga
     1981 cctacatcag ctatctgcgg cgcaagctgg acaccctcgg cccgccggtc atcaccaccc
     2041 ggcgcggcgt cgggtacggg ctgacgtgac acgccggata cgcctgccgc accccggccg
     2101 cccccgggtg cggtcgctgc gcgcgcagct cacgctggcg aacgtggtgc tgctggcgct
     2161 gggcatcgtc gccgcgaccg cggtgagcgt cctcggcatg cgctactacc tgctcgacca
     2221 gatcgacacg gagctggtcc gcacccgcga ctccctcggc ggctccgagc tgacgctgcg
     2281 gcagatcgac tcgctgagcg cgctgcgctt cctccgcgac cgggtggacc cggagtggaa
     2341 ccaggacgac gacgcccccg actcggtctt cgccgccgtg gaccgcgagg gcgagccggt
     2401 ccccgtgttc ggcttcgccc cgtcccgggc gcagcacgac ctggcggacg ccgtgggcga
     2461 cccgggcgcg ctgatccggg acccggagcc gcaggacctc accgtgcgcg gcaacgccta
     2521 ccgggtcacc gccgcccgcc tcgccgacgg caccggtgtc ctcatcgccg gctccaccga
     2581 gccgctgcac gacggcgtcg ccaaggcggt caggttcgat ctggccatcg ggacgctgct
     2641 gctgctggtg ctggcctgcc tcaccatgat ggcggtacgg cggcggatgc ggccgctgga
     2701 ggacatggtg gagacgtcgt cggcgatcgc cgagggcgac ctgacccggc gggtgccctc
     2761 cagctgcgac acctcgctgg aggtggagca gctgcggctg gcgctcaact cgatgctcca
     2821 gcaggtggag tcggcgtacc gcacccggga acgcagcgcg gcgcagctgc ggcggttcgt
     2881 cgccgacgcc tcccacgagc tgcgcactcc gctcgcggcg atccgcggct acctccagct
     2941 ctacgagcgg ggcatgctgc gcgaccccac ggagcgcagg agggcctggg accggatgac
     3001 gaccgagtcc gaccggatgg gacggctggt cgacgaactc ctggtgctgg cccgcctcga
     3061 ccagcgtccg gagctgcgga agcggaacgt ggacgtgtgc cggctggcgc gggacgcggc
     3121 gcaggacctc cgggtgcagc aaccggaccg tccggtgcgg gtggacgcgg agggtgcggt
     3181 cctggtccac gccgacgagg cgggcctccg ccagatcctg ggcaacctgg tcggcaacgt
     3241 ccgcacgcac acccccgccg acgtgccggt acggctcggt gtggcccgcg tggacggcga
     3301 ggtgcggctg tgggtcgcgg acgagggccc gggcctcgcc ccggaggacg cgggccgggt
     3361 cttcgaccgc ttcttccgcg cgggcggcgg cgcgggcagc ggcctgggca tggcgatcgt
     3421 gcagggtgtc gtgcacgccc acggcggcga ggtcacggtg gacacggccc cggggaaggg
     3481 cctgacggtc acggtgacgc tgccgccgtg cacggagacg ttcacgcggg cggactgagc
     3541 gggcgccctg agtggggccg gccaccccgg ggagcgcggc aggcggacgc tccctccgcg
     3601 gcccagggag ccgagaccgg gcccgccgac cggccctacg gcccgatcgc gaaccggccg
     3661 accggccgga ggccgagcat ccccgggcag cacgccgatc gccggccacc ccggggaaca
     3721 gcctgggccg accacaccca ccggccccat cccggggcac cgccggtcgt cccccgcggg
     3781 ggacccgccg gcccgtcgcc ggagggctac gcctggcggg aggccagttc gatcaccgtg
     3841 atgtcggagg gggcgcccac gatccgatgt agtgatccca acaaacctgg ccaacggatg
     3901 gctaacataa ggccaatgga ttccccatgg gtcacccgga aggcaggggt ggggcgtgac
     3961 acggcgcggc ctgcgcttcg gactgcgggg gacgcctgtc ctctacggcg gtgagccgtc
     4021 ccccggcggg ccctcctccg acgggggcgc cgccgcgtgt gcagggggcg tgagggggta
     4081 ccgcgtcacc gggacccgcc ccgtcggagg agggctcgcc gggggacggc tcaccgccgt
     4141 agaggacagg cgtccccagc agtccgaagc gcaggccgcg ccgtgtcacg ccccacccct
     4201 gccttccggg tgacccatgg ggaatccatt ggccttatgt tagccatccg ttggcaaggt
     4261 ttgatgtgat cactacatcg gatccggccc gggggtgcgc gcgtgacgcg caactcgggg
     4321 gagtgtcgcc gccggccgat ccgcttcacg aggacccggt cgccagaggt gaacgaccgg
     4381 ggcctcgttc ttttcggccg cagcgacggc cgacaccggc cccgggtgct cacgaacgcg
     4441 tgagcaggct cgttcgcggt cggagagaag gccgcgtcat gcggcctcgc cgcccggcgc
     4501 aggctggtcg tcacggccgc ggacggttcc gtggccgccc tcggcaccgc cgacggccgg
     4561 caggtgtggc agcaccggtc gctgaccgac tcggccgggc cgacgtatac cggtcgggcg
     4621 gcagccgatg cgtggccggc agacggctgg ccgcccacgg cctcgcccac ggcaaggaac
     4681 tccggtcgac cggccccctg gtccggccca gcggcgcccc gcctgctgga gctctccgac
     4741 cttcggcggc tggaccgtgt acgcgaccga ggtcgtcttc ccggcgcggc tcggcctccg
     4801 cacgggccgg cgcaccgagt ggatcgaaac cgtgcgaccg gctgcggagt gccacccagg
     4861 tcaggccggc ggcatcccac gctcggcgat gccgctctcg taggcgacga tgaccagctg
     4921 ggcgcggtcg cgggcgttca gcttcgagag caggctgctc acgtgggtct tcacggtggg
     4981 caggctgatc accagccggt cggcgatctc cgcgttcgac aggcccttgg cgatcagcac
     5041 gagcacctcc tgttcgcgcc gggtgagccc gacgagccgg ggggccggcc gggcggccgt
     5101 gtcccggtgg gagaagttcg cgatgacgcg acgcgtgacc gagggggcca gcacgccctc
     5161 gccggtggcg atgacgcgga tgcccgcgac gaggtcctcc ggcgaggcgt ccttcagcat
     5221 gaagccgctc gcgcccgcct ggagggccgg gtagacgtac tcgtccatgt cgaacatcgt
     5281 gagggccagc acccgcacgc ccgccatgga cgcgtccgag cagatctcgc gggtggcggc
     5341 gattccgtcc atgacgggca tccgcaggtc catcaggatc acgtccggcg tcagttcgcg
     5401 ggcgagcgcg acggcctcgg cgccgtcccc cgcctccccc accgtctgca tgccgggagt
     5461 cgagtcgacg aggacccgga agctcccgcg cagcagcgcc tggtcgtccg cgatcaggac
     5521 ccggaccgcc tcggccattg cgcatccctc gtgcctcggg tcgccgccgt gcccggcggt
     5581 ccgacgcctc gtcagcctgt ctcccccgca ccttatcggc cgggccggtg gccgccgtca
     5641 ggtttccgcc gccaccagtt cccccgtccc gtcgtccccg acggtgacgg tgacgccgtg
     5701 gggctccgcg agccgcgcga agtcggtgag gatctccttg gcgtcgaccg gaccggccag
     5761 ccggggccgt ccccgccggt gcacgggccg cccgaagccc aggtcgaccg ggtgcagccg
     5821 ggcgccggtg aagaccccgt cgtcgaaggt gagcaccggc acgagcgact gccagtaccg
     5881 gcggtgcggg gcgaacatcc gccggccgtg ggcggtgatc cgctcgaagt acccgccggg
     5941 ggtcagggcc tcgtccgtgc cggcccgcgc gtagtcctcg gcggggatgc ggtcggccag
     6001 ttcgatctgg ctgacgatgt tccccaggct gtggaagacg ggccggcccc ggtggagctc
     6061 cacaccgcgc agcatgtgcg ggccgtgccc gaccacggcg tcggcgccgg cgtcgatcac
     6121 cgcacgggcg aagtcccgca ggaactcggc gggcgtctcg ggcgtcgggc cgggttcgtg
     6181 ggagtggacg ctgaccagga ccaggtccgc gcggcggcgt gcctcggcca cccagcgggt
     6241 gatgtcgtgg acgtcccggg ggtcgcaggc ggtggtcagg cccggggtgt ccgcggtctc
     6301 gaaccgggtg ccgaacagcg cgagccggcc cggcccgggc atggcggggt cgaacccgag
     6361 cagggtgcgc gcctcggccc gccgggaccg cagcccggtg gcgccgtcga tctcccgcaa
     6421 cgcctccagc tgcgcggccg tcacccgcag cgtcacgccg tgccgcagcg ggctgagtcc
     6481 cggccggccc ggcaggtcgg gggacggggc ggccgcctcc tgcccgtccc ggaaggtggc
     6541 ggtgcacgcg atcagcgcca ggctgccggc cggccggtcc acgtagaccg ggcgccgggc
     6601 cgcggtgagg tcggcgccga tccccgcgaa cggcaccccc ttggcgcgca gcaggtccac
     6661 cgtgccgagg acgccctccg tgccgaggtc gagggcatgg ttgttggcgc agctcagggc
     6721 ggagaagccc atccccgtca cgtcgtcgag gacggcgggg tccgcgatca ggccgcctcc
     6781 gctggccgcg tcgggtacgg ggtggccgcg tccggtggcg ggcgcgacct ccaggttggt
     6841 gaaggcgaag tcggccgtgt gcagcacctc gcccagtcgg ccggcggcgg ggtcggaggt
     6901 gagcggcgcg ccccgcgtca tcatgcagtc cccggccagg gccacggtca gacggctcat
     6961 gaacggctct cctccaccgc tcgcacgaac gccttcgccc ggtcgggtcc ggagaggtcc
     7021 acgcgcttgc cggccacctt cacggtcggg gtgagggcca ccccgcggga ggcggcgacg
     7081 tcggacacgt acgtcaccca gggccggtac gtgccgtcgc ggacgcaccg ctcgaaggac
     7141 gcgccggtga tgccgacgtc ccggccgagg gcgaccaagc ggtcggtgtc gaggccgggg
     7201 ccctgctccg gcggctgctc gtcgaagagc gccgccgtgt agggctcgaa cttgttccgg
     7261 tcagcggcgc aggcggcggc ggacgcggcc cgggtggagt agccggcggg ggcgctgcgg
     7321 tcgtcgagga aggcgaccgg gtggtggacg acgcgtacct ctccgcgttc ctgcatctcc
     7381 ctcagttcgg gcgcgagggc cttctcggtg atgcggcact cggggcagag gtagtcgagg
     7441 tagaggtcga cacggaccgg accctcggac gtgacgagtc cggcccggtc ggcggccacg
     7501 ccggcgggca cctcctcggg cgcatcgccg ggctcggtgt cggtggcccg gaccaggccg
     7561 gcgccgacga gcgcggcgcc gagcaccgtg accaccgccg cgagcgacac cagcacagtg
     7621 cggcggcgtc gccggcgccg gtggaccatt tcccggacct gggcgtccgt cgacggctgc
     7681 atgtctatcg ctccttcgtt tccaggacca agcggtccag ggcgtaacgg gtccgcgggc
     7741 gggtgatcag gaacgcggcg gcgccgagga aggcgaggtc gcgcgcgatg tcgacgacgt
     7801 agccccgttc cgcaccgccg gtgagggtgc cgccgctgct gaagcagccg cagtcgatgg
     7861 acatcccgcg cgcccacacc gagacgatgg ccgcgatgta cgtgaccatc agcagggcgg
     7921 tgacggccgc cacggcgcgg gtggcgagcc cggcgagaag cagcagggcc agcgccagct
     7981 cgacgaacac cacggcggcg ccgacgagtt gtgcgctttc ctcggggacg agctggtaga
     8041 gggcgacggt acggccggct cccgtgaggt cctggacctt cacccagccc gcgtagccca
     8101 ggaccgccgc gaggacgatc ctggccgcgg tgcccgccca tgtccgcacg aacgtcacgg
     8161 cccggccccc tttcactgcg cccgggagaa ccggtaggtg gcgaggccca gtacggcggc
     8221 ggtgagggcg gccagcacgc cgagttccgc gtacacgggc gggttccagt cgccgagcat
     8281 cagccgggtc tgctccgagg tgaggacgtc ggggccgggc agcgtccggc gcacggcgtc
     8341 gacgccgtag gtcagcgggt tcagcttgac gacggtgtcg agccagcccg gcagcccgtt
     8401 gggcgggaac atcgcgccgg agaagaacat cagcggcatg aggctgaggt tcaccacgat
     8461 ctggaaggtg tcgacctgcc ggatggtgac cgcggcgaga aggccgaacg aggtgaagag
     8521 cagggagacg aggagcagtt cgacgaggac gaccagcagc atcggcgtgt accggacgcc
     8581 cgcgatgcct ccgaccgcga gcagggcgag gccgtagacg gcgcccgtgg tggctccgcc
     8641 gagcgcgagg ccgaggacga tgctcgaccg cgggaacggg gagacgagca tctggcgcag
     8701 catgccgagt cggcggtccc agacgagcga gatgcccacg gcgatggccg gggcctgcac
     8761 ggacatcacg agcgcgcccg ggaacaggta ggcccggtag tcgttcagct gggccttgcc
     8821 gagcgtggac gacgccgctt ccagccccgt gcccagcacg acgaggaaca gcagcggggt
     8881 gaccagaccc atgacgaggc gcagcggatt gtgccgcagc cgggtcatct cacgccgcca
     8941 cagcagcgcg tagggccgta cggtgtgcca ggtccgcccc tcggacgagg gcggggccgg
     9001 tttcgtcacg gtgacactcg gcgggatgtc ggtccgggtc atcgcagtcc ctccccgagg
     9061 ctgtcgagcg agcgggcgtc ggacccgctg tccctgatcg cggtcccggt gtggtgcagg
     9121 tagacgtcgt cgagcgaggg cggggtgacg gtcaccgacc gcacgcccag gcccagcccg
     9181 gtgcacaggc ggggcaccat ggaggcgccg tcctgcaccc tcagccgcag cccgccgggg
     9241 gtgttctcgg ccgtcagccc gaagcggtcg gacagcaggt ccacggccag ccggtcgtca
     9301 tcggtgcgca ggtcgacgac gtcggccccg atgacggact tcagctccgc gggtgagccc
     9361 tccgtgacca gcttgccgcg gtcgaagatc gcgatgcggt cgcagtgctc ggcctcctcc
     9421 agctggtgcg tggtgaagaa gaccgtgatg ccccgctcct cgcggagccg gtccaggtgg
     9481 tgccagacgg ccgcgcgggt ctgcgtgtcg aggccggtgg tcggctcgtc gaggaagagc
     9541 acgcgcgggg agccgagcag gccgcgggcg atctccaggc gccggcgcag gccggtggag
     9601 aagtgccgta cgggggtcct gtcgcgtccc gtcagctcca tcatgtcgag catcgcggcc
     9661 accgcggcgc gcgcctcggc ccggcccaga ccgcacaggt cggcctggaa gcgcaggttc
     9721 tcggtggccg tgagctccag gtcgagcgtc gactcctgga agacgatgcc gatcctgcgc
     9781 cgcacctcgg cggcgtgcgt ggtgacgtcg aacccggcca cctccgcgcg gcccgccgtg
     9841 ggcctgagca gcgtggtgag catgctgatg gtggtggtct tgccggcgcc gttcggcccc
     9901 aggaacccga aggtggcccc ctcggggacg ttcaggtgga ggccgttcac ggccggtgtg
     9961 gtgccgtagt acgcgtagag gccctcggcc cggatcgccg tcatgagccg cctccggtgc
    10021 ggcgcacggc cagcacggtc cggaacacgc cgacctctcc gggctcgtcg accgggcggc
    10081 cgccggcctc gacccaggcg tgcgcggcga agggctgcac ccggaagccg gtgcaccagt
    10141 cggcccacct gccccggacg cggagcagca gcacggtggc gacggaccgc tggaggcagc
    10201 cgaggcccgc gcagcgggtg ctgacggaga cgacggaccg ccgggcccgc gccacctggg
    10261 cgtacccggc gggccgggaa cccttgctca gtacgcgcag cacccggtgc agccgggccg
    10321 ggggcagcct gaccagcagg cgggcggcgc cggccgcgca tctgggggcg atctgccggt
    10381 gcagcgggag gcgcgtggcc tgttcggcga cggcgggcgt ggtcactgcg tcacctccac
    10441 gaggtcggcc tcgctcagcg aacggaccag ggccagcacg tcctggtgca cccgctcctc
    10501 gctgacgggg gcggactccg acaggctcac ggcggccgcg gcgggagtgt cgccgtcgag
    10561 cagcctgtgc aggatcgcgg agccggaggc gttcagctgg tagtagcggc cgcggcggcc
    10621 gtcgagcagg accgccccgg aatcgacggg cgtgagggtg acgtcgcggg caagactcag
    10681 tttcatcggt tctctccggt gtcctggggg tgcgcccgca gccagctctc gcacgccacc
    10741 gtggtctgga tcggctggag gtgatgggac atcggcccgg ggttgagcac cgcggaccgg
    10801 aaggcgtgcg ggtcgacgag gccgagccgg gccaggagcg agtcctcgca gaggtccagg
    10861 atccggtccc ggttgcgttc gatgccccgg aacgtctcgg cgctgaactc gcccttgtcg
    10921 cgacgggcga ggaagtcgtc gggcacgagg ccccgcacgg cggtggtcag cagcggcttg
    10981 aaccgtccgg cggccagccg ctcgtcgatc cgggtggcga aggccgcctc gagaacccgg
    11041 tcgtcgagga acggcgcctc ccagaccagg ccggtgccgg cgacggcggt gttgacctgc
    11101 cggacggtgt tcccctcgaa cacgatggag gacagcgcct ggtgccgggc ccggtcggcg
    11161 tccagcggtt ccggggtccg ttcggctgcc gtggtcagca ggtcccgcac ggcggccacc
    11221 gcgtccgggg tggcccaggc gggcatgcgc ggcgcgaaca cccagccgaa gtcgggctcg
    11281 ttgaccggcg ggggcggggc ggtgatccgc ccggcgacct cggtcaggtt ctgcgcgaac
    11341 gtcgaacggt ccgcgagctg ccgcagggtg ctgcgccagg gccagcggtt ggccagccgg
    11401 tagcggttca ccagccgcag gccgtcggcc gggcggaggc gggcgaggga ccaggcgcag
    11461 gcgggcatcc ggccgaagag ttcgtccccg ccgaacccgg acaggtgcag ggccgccccc
    11521 tcggcggtgg cccgccccgc gaggtcccgg atgtgcgcga gtccggaggc ccacgtcgag
    11581 gggccctcgg gggccgcgtt gacggtgtcg gcggtgtagc cgatgtcgaa caggttggcg
    11641 gcgcggtcag cggcgagcat gtggtggcgg gcgtgcggca gatgcgcggc ggccttgcgg
    11701 gcccatgcgg tgtcctcgtt ggcctcgtcg agcggcatga cgtggtaggt gaccaggtcg
    11761 gcgccggccg cgtcggcgag gaagcacagg gtggtcgagt cgagtccgcc ggagaggtcc
    11821 gcgctgatcg tgcggtgcgg gtccacacgg gtcgcgaggg cgtcggtgag cgcggaacgg
    11881 acggccccgg caccctcctc cagcgagagg gccgcctcgg gcagttccca ccaccggacg
    11941 ctgcgggccc ggccgtcggc gccgagctcc agccagtggc cggtgggcag cgcgtccacg
    12001 ccgcggcgga ccgggcgcgg cgagagcggc cagggcgccc cgccgggcgc cagcagccgt
    12061 gcggccagca gggtctcgtc gagccccgcg ccggtgaggc gcagcagcgg gtccacggcg
    12121 ctcgcggcga ccgtcacccc gtcgacccgg gcggtgaaga cctgccgtac gccggccacg
    12181 gagccctgga ggcggaccgc gccgtccagg gaggccgcga ggtggaagac cccgggcagc
    12241 cgggacgcga cccggtcgac gtcgtgcagc gagcgcatcc ggccgagcgc ggaggccgtt
    12301 cccgcgtcgt cgggccgggt gtgtccgaac accgccaggc ggcgggtgcc ggcctcggtc
    12361 accgtcgtcc cgccctcggg ccagtccccc acgatccagg gccgtcccga cgcgtggtcg
    12421 atccgcttcg gtgccgccac ccggtccgcc agcgcggcgc cggcgggaca gtccggaagc
    12481 actgtgaatt ccatgtcgcc tcactccccc gggcggtgcc ggtgtgccac gaggggcaca
    12541 ccggcaccgg tgcgatcacc agaagcagac gacggcgtaa ccgcagccgg cgaagtcgtt
    12601 gcagctcccg atgccgaggc acttggtgag ctcgtcgaag tcgccgatct cctgcagggc
    12661 gggcggctcg tagatcgcgg tcatggttcc tcccgtgggt cgaacggccc gggccgacct
    12721 tttcggcccc gtgacctgtc cgggaaccta cgaggggtca tgtcccacgt gcatggggca
    12781 catgactcag gctcgtcccg tcacccgtcc cacgaacggt gccggagcca gccggccgtc
    12841 agggcggccg cggtccacgc gagcagcacg acgaggccgg tgagggcgtc gccggagtcg
    12901 acggtgagca tctgggcgcc ggcccggtcg gggaaccagc gcgccacctc cttggtcgcc
    12961 ccgatgacgg acaggatctg cgagccgacc agcaccagcg gcagcagcac ggccagcggg
    13021 aggacggcac tgcgggcggc ggccgccacc cctacggcga gcagactcat gagggtcagg
    13081 tagacgaccg cgccggccag ggcgcgcggg acgccgtccg cgtcgagcgt cgacccgtga
    13141 gggcccaggg cgagttgggt gaccaggtac gacacgaccg tgaccggaag ggcgacggcc
    13201 agggccgcga gggcggtgac ggccgtcttg gccaggtaga gccggccccg ccggggcacc
    13261 gcgagcagcg agacccgcat catgcccgag gtgtactcgc cggacatcat cagcaccccg
    13321 aacacgatca gggcgagctg cccgtagagg atgccgtcca ggccggcctg ctcgggcgtg
    13381 aagtcggagc gcagcccggg attgccggac tcgatcgcgc ccttggcgga ccagccgccc
    13441 agtgcggcga tgaacacgct gacggcgacg aacagcagca gcgcgacgga cgtggcgcgc
    13501 accgcccgca tcttggtgaa ctccgcccgc gcggccgcga gggtctcaga catgcgtggg
    13561 atctcctccg tcgtcgacga gcttgaggaa ggtctcctcc agcgagccgg tgtgggtgct
    13621 cagttcatcg acggggacac cggccgccgc ggcgatgcgg ttcacctcgc ccgccggcag
    13681 gccgtccacc tccaggccgc cgtccgcggc ccgccgcacc gaggtggcgc ccttcgcctg
    13741 gaactcctgc gcggcccgca gcggttcgga ggtccggacc cgtacgaacg tccggccgtg
    13801 acgtccgagg aactccgaca cgctggtctc ggcgaggagc cggccccggc tgatcacgat
    13861 cagctcgtcg gcgacctgcg ccatctccgc catcagatgg ctggagacga ggacggtgcg
    13921 gccctcggcc gccttggcgc gcagcagttc gcgcagccag cggatgccct cggcgtcgag
    13981 cccgttgacc ggctcgtcga gcaccaggac cggggggtcg ccgaggaggg cagccgccag
    14041 gccgaggcgc tgtcccatgc ccagggagaa ggtgccgacc cgcttacggg cggcttgcgt
    14101 gagaccgacg gcgtcgagca cctcgtccac ccgccggcgg ggaacgcggt tgctgcgcgc
    14161 cagccacgac agatggccga cggcggtcat gccacggtgc ggggccgacg tctccaggag
    14221 cgcaccgacg tgccggagcg ggtaggtcag gtcgcggtag gcgcgtccgc cgacggtggc
    14281 cgtgccgccg tcggggcggg tcaggccgag catcatccgc atcgtcgtgg acttcccggc
    14341 gccgttgggc ccgaggaagc cggtgacgac acccggtcgc accgtgaagg acaggtcgtc
    14401 gacgacggtg gcgggcccgt accgcttggt cagtctctcg gcaagcatca cgtgtcgagt
    14461 ctcgcggcgc gcaccccgcg accgcatcgg tccgcggtcg ggaacccggc gcggtgccgc
    14521 gcgggcgccc ctcatacccg gggatgagcc ggtgtccgtc agccctggaa cggcagggtg
    14581 gccgtcaccc ggaaaccgcc gccgggccgg gggccggcgg cgagggtgcc gccgtgcgcg
    14641 gcggcccgct ccttcatgcc ggccagcccc atcccgccgc cgggcaccgt ggtccggcgt
    14701 ccggggccgg ggccgtcgtc ggtcacgtcc accgtcagca cgtcctgccg cgccgtgacg
    14761 tccacccggc agccggtcgg cgcggcgtgc ttgagcacgt tggtgaggga ctcctggacg
    14821 atgcggaacg ccgccagcgc gacaccctcg ggcggttcct ggtcgtagcg ggtgcgcagt
    14881 tcgacgcgga cgcctgccgc ctcggcggtc tcgagcagcg cgggcaggtc cgcaaggccg
    14941 cgggcgggcc gcaggtcccc ggcctcgctc cggtcctcac cgcgcagcac ggtcagggtg
    15001 gcgcgcatgt cgcgcagcgc ccggcggctg acgtcctcga tgaccgccag ggcctccttc
    15061 gcctcctcgg ggcgggtggc cacgacatgg ttggcgatgc cggccttgac ggcgatgagt
    15121 cccacgctgt gggtgacgac gtcgtggatg tcgcgggcga tacgcaggcg ctcctcggtc
    15181 ttcgcctgct cggcggcgtg ctcgatggcg cgccgggtac tgcgtctgcg ttccctgacc
    15241 gccgagccgg ccgcccaggt cgcgccgagc acgaggaggc cgaacacggc ctgcacggcg
    15301 gtgctgccac cctggtagga ctgcccgccc gacagcgtga ggagggcggc gaccgccgcg
    15361 cacagcccgg cgacggcgac ggccgacacc ccggggtcgc gccgccgggt ggtggccacc
    15421 gtgtggacgg cgtaggcggc ggccaggaac gaggcgggtc cgaggccggc ggccagcgcc
    15481 cagcaggcgg cggccaggac gaggacgaag accgggacgg gccacaaccg tcttacggcg
    15541 agcgggagtc cggcggccag ggacagcagc cacaggaggc cgccgcccgt cccgccgtcg
    15601 acggccggcc gcagcagcga cacctgcggc aggagcgtga acgcgcaggc cgccgccgcg
    15661 tccagcacga tcgcacgcct ccgggacccc tcacccatgc cgtcgaacgt aagcgctcgc
    15721 tccacggccc gtccaacggg cgcagggcag ggcgtccgcg tgtcgcgccg ggcgccgacc
    15781 gtttcggaca gtgtcgtccg ccccgcacca cggcccacac gggcgcgcgg tcctgcgggt
    15841 cgcgcgcccg gagccgcccc caccaccgcc gacgccggaa tcgcggtggt tcggcggcgt
    15901 ccggccgata ctcacccctc ggacaatacg ggtgcgttgg cgaccttgcg ttccctccgg
    15961 acgcgtccac atactcggca tcacgagcgc cttgcgcgct ttcgacgcac caccatgcct
    16021 gcgccgacag gtcatggcca cgggggccgc tgaagaccac aactgaccac gtcacgcccg
    16081 gagtcggtcg cctacggacc gacccttcgt cgcgtgtgcc cccggggacc gtccggcgcc
    16141 gtccgcacca cgccgacgca tttctggggg atgtacgaga tgaccgtcaa cctgttcgtg
    16201 gtcagcgaca acgcggtgtc cctcgccggg ctggaagcga ttgtcgatca ggatgaggag
    16261 tgcgctcttg tcggccgtgc cacggacgac tcggacgacc tccgacgtct gtcggacctg
    16321 gccggcaccg ccctccaccc cgacgtggtc gtcctcagcg aaccgtcctc cagcagaacc
    16381 gtctccgcac gcgtccggca catcgcctcc gccttccccg acgaggcgtc ccggccgcat
    16441 acgatcgtgg tggcgcagaa cgacgacgac gagatgatcg tctcggccct ccgcgcgggt
    16501 gtcaacggct acctctcccg cctcggttcg gccgacgaac tgctccgctc cctgaaggtc
    16561 gtcgcccggg gcggcgccgc cttctgcccc accgtcgcgg cccgcatgag ccgctacttc
    16621 tccgccatgc agggcctgca cgacggcgcc gggttcccgg ggctcaccag ccgcgagctg
    16681 gagatactgg agctcctggc cggcggcatg gggaaccggc agatcgcccg ccggctcttc
    16741 ctggccgaga agaccgtccg caactacgtc tcgcgcatct tcgccaagct cgaggtgcac
    16801 gaccgggcga cggcggtggt gatggcccgg gacgcgggct tcggcgagcc cgccggggaa
    16861 ctggtcccgc acggggcgct ctgagcaggc cacccactcg gctcagcccc cagcacgact
    16921 ttactaccct tggtaggatc cagaacatga gtggtagcaa gaagtactcg atcagtctcc
    16981 cggaagacct cgctgaggcc gtgcgtaccc acgtaggccc tggcggcttc tccgcttacg
    17041 tggccgaggc cctggagcag cgcgtagcga tggacaagct tcatgagatc gtcgccgact
    17101 tcgagaccga caacgacccg ctgtcccggg aagagatcga agcggcccgc gccgtactgc
    17161 gccacgaccg gcacgactcg agcggtgccg cggcctgatg cccggcacgc tcatcctcga
    17221 cagcgagggc ctgtccaagc tctacctcaa ggaccgcacg gtcctggcct tcatccaagc
    17281 agccacggaa gaaggcaccc gagtggccac gagcgccatg accacgcttg aggcggacta
    17341 cgagcgcgtc caccccgccc gcatccggtg ggtactctcc cggatcgacg tccacgacgt
    17401 caccgaggcc gtcaccggcc aagcgtcagc actcctgcgc gaccaccgcc tcagtggtca
    17461 caagtacgcc atcgacgccg tcctcgcagc cgttgcacgc tcggcgccag gaccagtcac
    17521 cgtcctcacc tccgacccgg aggacatcgg tctgctctgt ggatccgcca tcgaggtcat
    17581 caaagcctga cgcgtgcaga ccgcgggcac acccgctcac gcgtgacacc ccgcaagccc
    17641 tcgaccactc gcgccgatca gtcgccggag cgcaaaagcg ggtttcttcg cgcctgcttg
    17701 ccctcgtatc ccagggaggg gaggcacgtc cagttacaga ccatccggct tgccggcgaa
    17761 tcgtcctttc agtgcccccc aggcgggttc ggccggcggg ccgatgccgt cgctgatggc
    17821 tccgatgagg agggcggcgg ggggagtggg ccggcactgg gcggtgttga ccgcccaggt
    17881 gaagcccatg ccgacgcccg cgtcggtgtc ggccacacga ggccccgtcg aaacgatacc
    17941 tatcgcggcc ggtggacggt atcgtttcac tgaccgtggg catctgtgaa gggactatcc
    18001 acatggccag gaccgtcatc gacctcgatg aagacatggt caccgaggcc atgcgtatct
    18061 tcgggacgaa aacgaaggcc aaggccgtcc gcctcgccat ggaggacgcc gtcaagaggc
    18121 acctgcggca ggagggcttc gacgccatgg acgccggcga gctcgacttc agtgagatcg
    18181 tcgagaacac cgggccccgt aacgcagacg gctcactcaa gcacaacggc gaccgcgacg
    18241 gaggccgggc cgcctgatgc aggaccgcta cctgatcgac aaatccgccc tggcccgctg
    18301 gacgaagccg agcgtcaagg aagtgctcaa gcccctgcac gagcgctgcc tcctcgccgt
    18361 atgccggccc accgagtacg agatgatcca ctccgcacgg gacagctcag aagcaacacg
    18421 aatcagcacc tggctccacg ccttcgacta cctccgcatc gacgacgaca ccttcacccg
    18481 tgctctcgag atccagcgcc atgccctcaa cgcgggcttc caccgggctc tgtcccttcc
    18541 cgagctgctg atcgccgcca cagcagaact gaaccggcga actgtcctcc actacgacgg
    18601 agacttcgac atgatcgcct ctctcaccgg acaacccacc gaatgggtcg tcccacccgg
    18661 cagtgccgac cgctgagcag acacgttcgc ggtgctcacg cacacctcgc agtcgcgaac
    18721 ccgcaacgat atcggctcgc caccggtgcc atccgaaccg gtccccagcc cggagtgacc
    18781 gtcaccatgg acggggcccc gaacgaggga catccaagtc cgcgagatga gctgcagcgc
    18841 ctgtcgatag tctgcacgaa tgtgagcgag taccagtact acgagttcca ggccgtcgac
    18901 cgaccgttga cgaaggacga actcgaacgg gtgcgggcac tgtccagccg ggcccggatc
    18961 agcgcgacgc acttcgtcaa tgagtacgac tacggcgact tccgcggcga tgagcgcaaa
    19021 ctgatggagc aggtgtatga cgcccacctg tacttcgcca actggggctc gcggcgcctg
    19081 atgctgcgcc tgcccaccac actgctaccc gcccgcagcg ccgaaccata ctgcgtggaa
    19141 aacgccttga cctgctggac caggaaaggc cacctgctgc tcgacttctc ctacgaggcc
    19201 ggggacggcg gcgagtggga cttcgagacc tccttcaccc tcgcctcctt caccaccctg
    19261 cgtaccgaac tcgccgcagg cgacctgcgc cccctctatc tggcctggct gtccggactc
    19321 acccgctggg agctggagga ggacgtcgac gaggacgcgt acgcctccgc cctcgagccg
    19381 cccgtaccga ccggtcttgc ccacctcacc ggcccgcaga agagcttggc cgacttcctg
    19441 cacgtcgacc cacacctgct caccgcggcc gcccacgcaa gcggtgccgc cccttcccaa
    19501 gcgatcgaca aggacgagct cgccgcctgg atccgcgccc tgccgcaaca cgagaaggat
    19561 gccctgctgc tggacgccgc cctgggcacc gccccgcaac cgggccccgc gctgctggcc
    19621 cgccaccgcg ccgcgtcccg cggcacagcc gtgtcgtcca ccgctgcccg gcgccgctca
    19681 gccgcggaac tcctcgacgc cgcccacggg gtgcgcaccg agcacacacg gcgacagaaa
    19741 caggccggcg cccaggcccg ccgcgccgaa cagcgggccc gcgagagtcc ccacgttgcc
    19801 gatcgctcca tctgagaccc gtggatctca cccgtcgcca caggggcaac gagcgactcc
    19861 tgatggggcg tcacgcgcgg ctgtccaccg gttgtccacc aggactcttc acgccacgat
    19921 gatccatccc ccgcgaccgg gtaaaaggcc aggtcagacc ccttgccagt cgacttcgtc
    19981 acagaaaacg aacctccgac ctacgcatta cgatgcgcag accacgagtg cccgattggc
    20041 caggattcca aggtcaacga atacaccccc aggccaacga gctgacctgg ggtttacaag
    20101 agagccgcct tcgggattcg aacccgagac ctacgcatta cgagaccggg agcgatcatg
    20161 cccggggatg acctgccgta ccgtctcgtg acaattctcg cggtcagaac atctgcagca
    20221 gcttggcctg tgccatctcg tgccgcaccg cccaaaggcg tctgtccacc gcccgtccac
    20281 cggcgaggct gatcggccgc tcatctccgt ccgacctcgc ggctctcacc gaggaccgcg
    20341 cgcattgagc cgagggctac ggcacctgtg actttcctac aacgcgcggc cacccggcac
    20401 cttcgtaggc cccgccccgt acgacgcatc ggagtcctgg taccactggc gccgggcctc
    20461 cgatgcactg ccacaccatt caccggccct gggatacgcg ccccgcacct tctcacgagc
    20521 ggtgctgcag gccaccggcc gcaccgcgaa ggcgtacatc gtcgaacgga tcgccctgga
    20581 ggccacacgg ctcctcgcgc acgaccgtct caccgccgcc cgctgcgccg acgtgctcgg
    20641 ctttaccgac gcatccaact tctcggtgtt ctcgcgcaag gccacaggca tgcgcccggg
    20701 cacatggcag gcgacggtgg ctgccgagtg agggcgcgca cgcgcccggt gagccgcaag
    20761 tagttcgccc aacggccgaa accgcacgtg ggccaagtcg ccaccaaagg aaccttcccg
    20821 cgcggaggga tcggacccac cagcgccaaa cccgcttgac agcctgggtg ctggccttga
    20881 cggcgccgat ccgggcgatg cgcggcacgt ggaccggctc gggcccaccc cgttctggaa
    20941 gccctcatca aaatcgacac ggtgatgata aaaatcgaca cggtgtagac tttctgtcgt
    21001 cgggagcggt ccggctcctc gcaagacttg ctggaggaac agtgcagaag cagaactggc
    21061 tcatcaccgg cgtgagctcg ggcctggggc gcgccttcgc gcaagccgcc ctggctgccg
    21121 ggcacgccgt ggtcggcacc gtccgctccg agaaggatct gcaggccttc gaggaactca
    21181 ggcccggaca cgctcacggc cgcatcctgg acgtgaccga cggtgatgcc gtctccagcg
    21241 tggtcgcgga ggtcgagcgc agcgtcggat ccctggacgt cgtcgtcgcc aacgccggct
    21301 acggcctgga aggaaccttc gaggaaaccc cgctggccga ggtgcggcgg cagttcgagg
    21361 tcaacgtgtt cggggccgtg gccaccctgc aggcggcgct gccccacatg cgcgcacgcc
    21421 gccgcggaca cctgatgggc gtcacttcca tgggcgggct catggccgtg cccggcatgt
    21481 ccgcctactg cggcagcaag ttcgccctgg aaggcatcct cgaggcgctg ggcaaggaag
    21541 tcgcgcagtt cggcatccac gtgacggcga tcgagcccgg ctccttccgc accgactggg
    21601 ccggacggtc catgacacgc gccgcgcgga ccatcgacga ctacgacgag ctgttcgccc
    21661 ccatccgtga agcccggcag aaggccagcg gcaaccagct gggcaatccg gccaaggccg
    21721 gggacgccct cgtgcacatc gcttcgctcg agcgcccgcc ggcccacctc gtcctgggct
    21781 cggacgcgct gcgactggtc accgccgccc gcacggccgt ggacgaggac atccgcgcat
    21841 gggaggcgct ctcccgtacg accgacttcg ccgacggcgc gcagctctga tgcccggcca
    21901 ccaccccgcg cggagccgtc gcgctcccga acgcaagggc gacgtccggg agcgggccat
    21961 cctcgacacc tgcgaggcac tgctggcgca caagggctac gacgccatga ccgtcggcga
    22021 catcgcccag ggcgccggca tcacccgcgg cgcgctgtac ttctactacg gttccaagca
    22081 agaagtggtc acggcgctcg tggcccgaac cgtcgagcac ctgtgggagc ggtcgagggc
    22141 caccgcggag gccgacgagc cgcgccgggc catcgcggcg gccatgcggc gcacggtcga
    22201 gctgtggaac gagcacggcc tggtcatgcg cacggcgatc gacctgtcgt tgaccgtgcc
    22261 ggagatcggc gagctgtgga agcacactgc tgacctgttc atcgcggcca tcaccgccgt
    22321 cctggaacgc gccggcatcc aggccggcac cgcaccggac caagcctcag caatggcacg
    22381 tgccctgtgc tggatgatcg agcggacctt ctaccacgcc tcacaggaat cccgcgagaa
    22441 ccttcaggaa gcatcggaga catgcgaaca catctggctg accagcgccg gcct
//
