LOCUS       NZ_RAII01000001        10399 bp    DNA     linear   CON 15-MAY-2024
DEFINITION  Streptomyces sp. E1N211 s-211_S3_L001_R1_001, whole genome shotgun
            sequence.
ACCESSION   NZ_RAII01000001
VERSION     NZ_RAII01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. E1N211
  ORGANISM  Streptomyces sp. E1N211
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7318313)
  AUTHORS   Vicente,C.M., Thibessard,A., Lorenzi,J.N., Benhadj,M., Hotel,L.,
            Gacemi-Kirane,D., Lespinet,O., Leblond,P. and Aigle,B.
  TITLE     Comparative Genomics among Closely Related Streptomyces Strains
            Revealed Specialized Metabolite Biosynthetic Gene Cluster Diversity
  JOURNAL   Antibiotics (Basel) 7 (4), 86 (2018)
   PUBMED   30279346
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7318313)
  AUTHORS   Leblond,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2018) Dynamique des Genomes et Adaptation
            Microbienne UMR 1128, Universite de Lorraine - Institut National de
            la Recherche Agronomique, Faculte des Sciences et Technologies
            Boulevard des Aiguillettes BP 70239, Vandoeuvre-les-Nancy F-54506,
            France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics Workbench v. 6.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003671735.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 02:33:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,941
            CDSs (total)                      :: 6,866
            Genes (coding)                    :: 6,131
            CDSs (with protein)               :: 6,131
            Genes (RNA)                       :: 75
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 736
            CDSs (without protein)            :: 735
            Pseudo Genes (ambiguous residues) :: 0 of 736
            Pseudo Genes (frameshifted)       :: 138 of 736
            Pseudo Genes (incomplete)         :: 673 of 736
            Pseudo Genes (internal stop)      :: 17 of 736
            Pseudo Genes (multiple problems)  :: 87 of 736
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:49:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2575627
            Orig. end                         :: 2586026
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            RAII01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="8"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    20..34
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.36"
                     /tool="antismash"
     misc_feature    complement(364..378)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
     misc_feature    complement(1110..1124)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.48"
                     /tool="antismash"
     gene            1383..1991
                     /locus_tag="EBH12_RS11995"
     CDS             1383..1991
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="EBH12_RS11995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_114873426.1"
                     /transl_table=11
                     /translation="MDLDIPELPFPLRTYGPDGSWSYEDGVLTGWAGPRQDRFVPPTGE
                     SLEPAADAPRLLGAPEGDFQLIARVTVGFAGSFDAGVLYVHVGERAWAKLCLEYSPDVP
                     TVCTVVTRGHSDDANSFTVDGKTVWLRVSRTGRALAFHASRDGKQWIFVRLFTLGDEKE
                     TGAALVGFATQSPMGEGCVVTFDHIEYRLHWPPDLRDGS"
     gene            2095..2835
                     /locus_tag="EBH12_RS12000"
     CDS             2095..2835
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019522167.1"
                     /locus_tag="EBH12_RS12000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_114873425.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALASFPHRDGVEVVHRSFELDP
                     GRAKDDVEPVLTMLTKKYGMSQAQAEAGEDNLGAQAAAEGLPYRTRGRDHGGTFDMHRL
                     LHFAREHGRQEQLLDLLYRANFAEERSLFGDDERLVGLAGAAGLDPEAARAVLTDQEAY
                     AAEVRADEQDAARLGARGVPFFVLDRKYGVSGAQPVDVFTRALTQAWGERSPLTLVDGG
                     TDAEACGPDGCAVPERENTRGQNA"
     gene            2949..4001
                     /locus_tag="EBH12_RS12005"
     CDS             2949..4001
                     /NRPS_PKS="Domain: Aminotran_5 (17-344). E-value: 3.7e-24.
                     Score: 77.2. Matches aSDomain:
                     nrpspksdomains_EBH12_RS12005_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019522166.1"
                     /locus_tag="EBH12_RS12005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_114873424.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.3e-16, bitscore:
                     59.1, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METFESLVRAEFTPKNTYLNTASNALLPARAVAALDAAVRLRAEG
                     RPLDRLFADVEACRAAYARLADVPAERVAAGATVALFSSLVAASLPPGADVLTADDDFT
                     SVLNPFHARGDLKVRSAPLEGLADAVRPGTALVAVSAAQSADGRIADLPALREAARSQG
                     ARTYVDFSQAAGWLPVEADAYDYTVAITFKWLLGVHGAAFLTVPEDFGDLVPVQAGWTA
                     GEDPWGSCYGPVAELAHSARRFDTTPALFSYAGLRASLTLLEELGVDAVRAHDLALADR
                     FRAGLASLGHEPLPAPGSPIVSVPELGRLQPELSRAGIEVSNRAGNLRASFHLYNTAAD
                     VDRLLDALSG"
     aSDomain        3000..3980
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EBH12_RS12005_Aminotran_5.1"
                     /evalue="3.70E-24"
                     /label="EBH12_RS12005_Aminotran_5.1"
                     /locus_tag="EBH12_RS12005"
                     /protein_end="344"
                     /protein_start="17"
                     /score="77.2"
                     /tool="antismash"
                     /translation="YLNTASNALLPARAVAALDAAVRLRAEGRPLDRLFADVEACRAAY
                     ARLADVPAERVAAGATVALFSSLVAASLPPGADVLTADDDFTSVLNPFHARGDLKVRSA
                     PLEGLADAVRPGTALVAVSAAQSADGRIADLPALREAARSQGARTYVDFSQAAGWLPVE
                     ADAYDYTVAITFKWLLGVHGAAFLTVPEDFGDLVPVQAGWTAGEDPWGSCYGPVAELAH
                     SARRFDTTPALFSYAGLRASLTLLEELGVDAVRAHDLALADRFRAGLASLGHEPLPAPG
                     SPIVSVPELGRLQPELSRAGIEVSNRAGNLRASFHLYNTAADVDRL"
     gene            complement(4098..4994)
                     /gene="thpD"
                     /locus_tag="EBH12_RS12010"
     CDS             complement(4098..4994)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448161.1"
                     /locus_tag="EBH12_RS12010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /protein_id="WP_048458475.1"
                     /sec_met_domain="PhyH (E-value: 2.3e-39, bitscore: 134.5,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTTRVTDLYPTRGATEVVTPRQDPVVWGSPDTPGPVSAGDLQA
                     LDRDGFLAVDQLIGPDEVEVYKRELDRLVDDPAIRADERSIVEPKSQEIRSVFEVHKIS
                     EVFANLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPN
                     MRTISVSIALTENYDTNGGLMIMPGSHKTFLGCAGATPKDNYKKSLQMQDAGTPSDEAL
                     TKMASEYGIKLFTGKAGSATWFDCNCMHGSGDNITPYPRSNVFIVFNSVENTAVEPFAA
                     PIRRPEFIGARDFTPVK"
     gene            complement(5001..5399)
                     /locus_tag="EBH12_RS12015"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337952.1"
                     /locus_tag="EBH12_RS12015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_048458476.1"
                     /sec_met_domain="ectoine_synt (E-value: 4e-54, bitscore:
                     180.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKEIEGTDRHVKSASGTWESKRIVLAKEKVGFSVHETILY
                     AGTETDMWYANHVEAVVCTKGDAELTDRETGKTYHITPGTMYLLDGHERHTLKVKEDFH
                     CICVFNPPVTGREDHDENGVYPLLTEEV"
     gene            complement(5432..6703)
                     /gene="ectB"
                     /locus_tag="EBH12_RS12020"
     CDS             complement(5432..6703)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 8.3e-72.
                     Score: 234.0. Matches aSDomain:
                     nrpspksdomains_EBH12_RS12020_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="EBH12_RS12020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_048458477.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.2e-75, bitscore:
                     253.4, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRGWPTVFDRAHGSRMYDEDGHEY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLERDGVTHGLDMSTTAKRAFLESFQNYILRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GVPLVHGTPMPFDNYFDGQVPDFLWFERILEDQGSGLNKPAAVIVETVQGEGGINVARP
                     EWLRALSELCERQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGLPMA
                     LTLFKPELDVWEPGEHNGTFRGNNPAFVTATAALEAYWADGSAMEKQTRGRGEQVDQAM
                     IAITEENLADVKEYRGRGLVWGLEFHDKERAGRVAKRAFELGLLIETSGPESEVVKLLP
                     ALTITPDELDEGLRVLARAVRETA"
     aSDomain        complement(5606..6619)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EBH12_RS12020_Aminotran_3.1"
                     /evalue="8.30E-72"
                     /label="EBH12_RS12020_Aminotran_3.1"
                     /locus_tag="EBH12_RS12020"
                     /protein_end="366"
                     /protein_start="28"
                     /score="234.0"
                     /tool="antismash"
                     /translation="FDRAHGSRMYDEDGHEYLDFFAGAGSLNYGHNNPVLKRALIDYLE
                     RDGVTHGLDMSTTAKRAFLESFQNYILRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESVVSFTNAFHGMSLGSLAVTGNAFKRAGAGVPLVHGTPMPFDNYFDGQVPDFLWFER
                     ILEDQGSGLNKPAAVIVETVQGEGGINVARPEWLRALSELCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTVSKSISGYGLPMALTLFKPELDVWEPGEHNGTFRGNNPAFV
                     TATAALEAYWADGSAMEKQTRGRGEQVDQAMIAITEENLADVKEYRGRGLVWGLEFH"
     gene            complement(6772..7278)
                     /gene="ectA"
                     /locus_tag="EBH12_RS12025"
     CDS             complement(6772..7278)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004002246.1"
                     /locus_tag="EBH12_RS12025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_048458478.1"
                     /transl_table=11
                     /translation="MTAAQADLQIDRPTVADGAVLWRIARDSKVLDLNSSYSYLLWCRD
                     FAATSAVARDERGEAVGFITGYLRPDSPRTLLVWQVAVDEAHRGRGLAAAMLDALAART
                     AGEHGVDTVETTVAPGNTASERLFTAFAERHGALLEREVLFDTPDFPDGPHDPEVLHRI
                     GPFAL"
     misc_feature    complement(7303..7315)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    7394..7406
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.22"
                     /tool="antismash"
     misc_feature    complement(7435..7450)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7445..7460
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7445..7462)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.75"
                     /tool="antismash"
     gene            complement(7632..>7811)
                     /locus_tag="EBH12_RS12030"
                     /pseudo=""
     CDS             complement(7632..>7811)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004002247.1"
                     /locus_tag="EBH12_RS12030"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="DUF664 domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="GRDLCAGRPLDGTGRIADGPPAGTEVSPRWVLIHVIEEYARHNGH
                     ADLLRERVDGVTGA"
     gene            complement(7893..8993)
                     /locus_tag="EBH12_RS12035"
     CDS             complement(7893..8993)
                     /NRPS_PKS="Domain: Aminotran_5 (52-343). E-value: 1.7e-28.
                     Score: 91.5. Matches aSDomain:
                     nrpspksdomains_EBH12_RS12035_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019522160.1"
                     /locus_tag="EBH12_RS12035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_114873423.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.5e-21, bitscore:
                     75.4, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTNLLLDLPPLSAARFASIEDRVARLLGTGQDVLITQGEALLPLE
                     GAIRAAAGPGTTALNVITGPYGQTFGDWLRDCGATVIDLTVPFHTAVTAEQVRDAFAEH
                     PEIDFVSLVHAEAATGNTNPVAAIGEVVREHGALFYLDAVASVGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWARMAANPNAPRRSYLSLLDWKERWIDGGRKTLLHAP
                     AQLEMLALEACVERIEAEGPEAVMARHASAAAATRAGATALGGGIAPYVHDAADAAPVA
                     TTLRAPAGTTASDLVARALAADPGLPLAAGGGALAREMIRVNHYGPEATRGTVRACLAA
                     LATALTDAGVSVDLPAGLAAAERAWV"
     aSDomain        complement(7965..8837)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EBH12_RS12035_Aminotran_5.1"
                     /evalue="1.70E-28"
                     /label="EBH12_RS12035_Aminotran_5.1"
                     /locus_tag="EBH12_RS12035"
                     /protein_end="343"
                     /protein_start="52"
                     /score="91.5"
                     /tool="antismash"
                     /translation="GPGTTALNVITGPYGQTFGDWLRDCGATVIDLTVPFHTAVTAEQV
                     RDAFAEHPEIDFVSLVHAEAATGNTNPVAAIGEVVREHGALFYLDAVASVGAEPVLPDA
                     WGVDLCVIGAQKAMGGPAGVSAVSVSERAWARMAANPNAPRRSYLSLLDWKERWIDGGR
                     KTLLHAPAQLEMLALEACVERIEAEGPEAVMARHASAAAATRAGATALGGGIAPYVHDA
                     ADAAPVATTLRAPAGTTASDLVARALAADPGLPLAAGGGALAREMIRVNHYGPEATRGT
                     VRACLAALAT"
     gene            9098..9946
                     /locus_tag="EBH12_RS12040"
     CDS             9098..9946
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020121392.1"
                     /locus_tag="EBH12_RS12040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="WP_048458480.1"
                     /transl_table=11
                     /translation="MKTHPGRRTRVLVATTATAGMVLLAACTSTDDGGGGSTTAGGVEL
                     VKAGQLTTCTHLPYPPFQSEIDGEVQGFDVALIDLVADDLGVKQQILDTPFENFKTGAF
                     LNSGECDLAAAGMTITDERKKNVDFSDPYFNATQAVLVAKDSGVGSLADVKSENVELGA
                     QAQTTGEDYVKGEGFDPVSFESSDAVLNGLRTGQVKAVVIDYPVVQGWLKDKANADAFT
                     VAEQIDTGEQYGFTVKKGNKALQDAVNKALADAKADGTYEKLYEQWIGPYDESAASPAA
                     S"
     misc_feature    complement(10059..10074)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.98"
                     /tool="antismash"
     misc_feature    10311..10325
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.26"
                     /tool="antismash"
ORIGIN
        1 gcgttggtga ggccctcctg gacgatgcgg tacaccgcgt ggtccgtggc cggggcgagc
       61 ggccgcgcat cgccccggcg gcgcagctcc accggcgcgc ccgcgccccg cgacgacgcg
      121 accagcgcgt ccaccccggc cgcgccccgg gcgggcgtcg cccccgccgc atccggttcg
      181 gggccgggag cgggcgcggt cggcccctcg gtgtccaccc ggaggatgcc gacggcctcg
      241 cgcagctccc gtatcgccga cctggcggcc tcccgcagga tgcccactcc ctcccgctgc
      301 cggccgccga gctccgggtc cacctcgagc gcccccgcgt gcaccgcgat cagggcgagc
      361 tggtggccga ggctgtcgtg catgtcctgg gcgatccgct gccgctccag cagccgcgcc
      421 tgccgtgcca ccatcgcctg ttcgcgcacc agttgggcgt tgtgccggcg cagtgcctcc
      481 agcaggtcac ggcgttgcgc gcggtaccgg gaggcgagcc ccggcacgac cgccagcacc
      541 aggtaggggc ccgcgcccag ggccgccgcc atcagcagcg acagctccag cgcgtcctcg
      601 gccccgtccc cgagcagact gcccgcgacg tacagcacca gcccggcggc gtacgcgccg
      661 accgcgcgcc aggggcgctt gatgcggctg cccgccgacc agctcgcgta catgagcagc
      721 gggccggcgc cgagaaccga gccggccagg gcggccgaga cgacgagcgc ggtggcgggc
      781 agcgaccgcc ggaccagcga gagcagaccg acgagcaggg ccaccgccca gcccttcgcg
      841 gtggatccgc tctccatggc gtacatcggg cccgcgatgc ccgccagcac caggagcagc
      901 acggtctccc cggcgatccg gccccgcgac cagggggagg cggcggcagg ccgggacagc
      961 gagcggcggg cggtgcgaga ggtctgcgct gtgtccaccc gcccgaccct aggggcccgc
     1021 accggggcgc cggcatcctc cctccgtagc ggccccgaac gacgaaagtg aacggcgtcc
     1081 gtcaacgtcc gtatcaccat tgttcgttgt ggaccgcgac ttccggtgac tgcgcccgca
     1141 cggcccgccc cgcgacgatg gcggcgccgg cggggacctc cgccggcggc acaccccacc
     1201 gacaccggga gcacagcggt ggacgcgacg ggaccctgac ccggccggcg cttcaccgtc
     1261 ccacggaagg ccaccccgag gaccgaccgg tacctcgtca ccggggtcgc cctcgacacc
     1321 ttccggggcc gctgaccggg atccgccacg cgcggcgggc ccgctcctgg gacaatcccc
     1381 ccatggacct ggacataccc gaactcccct tcccactgcg gacctacggc cctgacggct
     1441 cctggtccta cgaggacgga gtcctcaccg gatgggcggg accccggcag gaccgcttcg
     1501 tgccgcccac cggcgagtcc ctggagcccg ccgccgacgc gccgcggctg ctcggcgcgc
     1561 ccgagggtga cttccagctg atcgcccggg tcacggtcgg cttcgccggc tccttcgacg
     1621 ccggggtgct ctacgtccac gtcggcgagc gggcgtgggc caagctgtgc ctggagtact
     1681 ccccggacgt gcccaccgtc tgcacggtcg tcacccgggg ccactccgac gacgccaact
     1741 ccttcaccgt ggacggcaag acggtctggc tgcgggtgag ccgcaccggg cgcgccctcg
     1801 ccttccacgc ctcgcgcgac ggcaagcagt ggatcttcgt ccggctgttc accctcggcg
     1861 acgagaagga gacgggagcg gccctcgtcg ggttcgcgac ccagtcgccg atgggggagg
     1921 ggtgcgtggt gaccttcgac cacatcgagt accggctgca ctggccgccg gacctgcgcg
     1981 acggaagctg agcgaggtca ggggcccgcc ggggaatgcc ggcgggtgct tgaacgttca
     2041 tccggtcgcg gaggacgtct ccgcagccgt gccgaccctg gagagagccc gcccatgcgc
     2101 gtcgagatct ggagcgatat cgcctgcccc tggtgctatg tcggaaaagc ccgcttcgag
     2161 aaggcgctgg cctccttccc gcaccgcgac ggggtcgagg tggtccaccg ctccttcgag
     2221 ctcgacccgg gccgcgccaa ggacgacgtc gagcccgtgc tcacgatgct caccaagaag
     2281 tacggcatga gccaggcgca ggccgaggcg ggcgaggaca acctgggcgc ccaggccgcc
     2341 gccgaggggc tgccctaccg cacccggggc cgcgaccacg gcggcacctt cgacatgcac
     2401 cgcctgctgc acttcgcccg cgagcacggc cgccaggagc agctgctcga cctgctctac
     2461 cgggccaact tcgccgagga gcgctccctg ttcggcgacg acgagcggct cgtcggactc
     2521 gccggcgccg ccggcctcga ccccgaggcc gcccgcgcgg tgctcaccga ccaggaggcg
     2581 tacgccgccg aggtccgcgc cgacgagcag gacgccgccc ggctcggcgc gcgcggggtg
     2641 ccgttcttcg tgctcgaccg gaagtacggc gtctccggcg cccagcccgt cgacgtcttc
     2701 acccgggcgc tcacccaggc ctggggcgag cggtccccgc tgaccctcgt cgacgggggc
     2761 acggacgccg aggcctgcgg cccggacggc tgcgcggtgc ccgagcggga gaacacgcgg
     2821 gggcagaacg cgtaggggcg gtacgcgcag gccggcgggt atgtacagcg gacgtataag
     2881 cgtcccttag cggtaccccg cagacatatg tgatggacgg gggcccggag gggtcgcaga
     2941 gtggtcccat ggagaccttc gagagcctcg tccgtgccga gttcaccccg aagaacactt
     3001 acctgaacac cgcgagcaac gccctgctgc ccgcccgggc cgtggccgcg ctcgacgcgg
     3061 ccgtccggct gcgcgcggaa gggcgcccgc tcgaccggct gttcgccgac gtcgaggcgt
     3121 gccgtgccgc gtacgcccgg ctggcggacg tgcccgccga gcgggtggcg gccggggcga
     3181 ccgtcgccct gttctcctcg ctggtcgccg cgtccctgcc gccgggcgcc gacgtcctca
     3241 ccgccgacga cgacttcacc tctgtgctga accccttcca cgcgcgcggc gacctgaagg
     3301 tgcggtccgc gccgctggaa gggctggccg acgccgtgcg gccgggcacc gcgctggtcg
     3361 cggtcagcgc cgcgcagtcc gccgacggcc ggatcgccga cctgcccgcg ctgcgcgagg
     3421 cggcccgctc ccagggggcg cgcacctacg tcgacttctc ccaggcggcg ggctggctgc
     3481 cggtggaggc cgacgcgtac gactacaccg tcgccatcac cttcaagtgg ctgctgggcg
     3541 tgcacggcgc ggccttcctc accgtccccg aggacttcgg cgacctggtc ccggtgcagg
     3601 ccggctggac ggcgggggag gacccgtggg gcagctgcta cggccccgtc gccgaactcg
     3661 cccacagcgc acggcggttc gacaccaccc ccgcgctgtt cagctacgcg ggcctgcggg
     3721 cgtccctcac gctcctcgag gagctcggcg tggacgccgt ccgcgcccac gacctcgccc
     3781 tcgccgaccg cttccgcgcc ggactcgcct cgctgggcca tgagcccctg cccgcgcccg
     3841 gctcgcccat cgtgtccgtg ccggagctgg gacggcttca gccggagctg tcgcgggcgg
     3901 gcatcgaggt gtcgaaccgg gccggcaacc tgcgggcgtc gttccacctc tacaacacgg
     3961 ccgccgacgt ggaccggctg ctcgacgcgc tgtccggctg agcgcacggc agcgggccgg
     4021 tgcctccttg tcccacacga ggaggcccgg cccgcagtcg gtgaggcggt gcgcacacgt
     4081 caccgccgta cgtgtcccta cttcacgggg gtgaagtccc gcgcgccgat gaactcgggg
     4141 cggcggatcg gagccgcgaa cggctcgacc gccgtgttct cgacgctgtt gaacacgatg
     4201 aacacgttgc tgcgcgggta cggcgtgatg ttgtcgccgg agccgtgcat gcagttgcag
     4261 tcgaaccagg tcgccgagcc ggccttgccg gtgaacagct tgatgccgta ctccgacgcc
     4321 atcttggtca gcgcctcgtc ggacggcgtc cccgcgtcct gcatctgcag ggacttcttg
     4381 tagttgtcct tcggggtggc gcccgcgcag ccgaggaacg tcttgtgcga ccccggcatg
     4441 atcatcagac cgccgttggt gtcgtagttc tcggtgagcg cgatcgagac cgatatggtg
     4501 cgcatgttcg gcaggccgtc ctcggcgtgc caggtctcga agtccgagtg ccagtagaag
     4561 ccgctcgcgc cgaagccggg cttgacgttg atccgcgact ggtggacgta cacgtccgag
     4621 ccgaggatct gccgggcgcg cccgacgacc cgctcgtcgc gcaccaggtt cgcgaagacc
     4681 tcgctgatct tgtgcacctc gaagaccgag cggatctcct gggacttcgg ctcgacgatc
     4741 gagcgctcgt ccgcgcggat cgccgggtcg tccaccagcc ggtccagctc gcgcttgtag
     4801 acctcgacct cgtccgggcc gatcagctgg tcgacggcca ggaagccgtc gcggtccagc
     4861 gcctggaggt cacccgcgga gacagggcca ggggtgtccg gggaacccca gacgaccggg
     4921 tcctggcgcg gggtgaccac ctcggtggcg ccgcgggtcg ggtagagatc ggtgacgcgc
     4981 gtggtcgtgg tcacggtgga tcacacctcc tcggtgagca gcggatagac gccgttctcg
     5041 tcgtggtcct cccgtccggt cacgggcggg ttgaagacac agatgcagtg gaagtcctcc
     5101 ttgaccttga gcgtgtgccg ctcgtggccg tcgaggaggt acatggtgcc gggcgtgatg
     5161 tggtaggtct tcccggtctc gcggtcggtc agctcggcgt cgcccttggt gcagacgacg
     5221 gcctcgacgt ggttcgcgta ccacatgtcc gtttccgtac ccgcgtacag gatcgtctcg
     5281 tgcacggaga agccgacctt ctccttggcg aggacgatgc gcttgctctc ccacgtgccg
     5341 gacgccgatt tcacgtgccg gtcggtgcct tcgatctcct tgaacgaacg gacgatcacg
     5401 gtgttgcgat gcctcctcgg ctggatgtgt ttcaggcggt ttcgcggacg gcgcgggcca
     5461 ggaccctgag gccctcgtcc agctcgtccg gggtgatggt cagggcgggg agcagcttga
     5521 cgacctcgct ctccgggccg gacgtctcga tcagcagacc gagctcgaag gcgcgcttgg
     5581 cgacccggcc ggcgcgctcc ttgtcgtgga actccaggcc ccacacgagg ccgcggccgc
     5641 ggtactcctt gacgtcggcg aggttctcct cggtgatggc gatcatcgcc tgatccacct
     5701 gctcgccgcg cccccgggtc tgcttctcca tcgcggagcc gtccgcccag tacgcctcca
     5761 gggcggcggt ggccgtcacg aacgcggggt tgttgccgcg gaacgtgccg ttgtgctcgc
     5821 ccggctccca gacgtccagc tccggcttga acagggtcag cgccatgggc aggccgtagc
     5881 cgctgatcga cttggagacg gtgacgatgt ccggcgtgat gcccgcctcc tcgaaggaga
     5941 agaacgcgcc ggtgcggccg cagcccatct ggatgtcgtc gacgatgagt agcatgtcct
     6001 ggcgctcgca cagctcggac agggcgcgca gccactcggg ccgggccacg ttgatgccgc
     6061 cctcgccctg cacggtctcg acgatcaccg cggccggctt gttgaggccc gagccctggt
     6121 cctccaggat gcgctcgaac cacaggaagt ccgggacctg gccgtcgaag tagttgtcga
     6181 acggcatggg cgtgccgtgc accagcggga caccggcacc ggcccgcttg aaggcgttgc
     6241 cggtcacggc gagcgagccc agtgacatgc cgtggaaggc gttggtgaac gacacgacgg
     6301 actcgcggcc cttcaccttg cgcgccagct tcagcgcgga ctccacggcg ttggtgcccg
     6361 tcgggcccgg gaacatgacc ttgtacggca ggtcacgcgg gcgcaggatg tagttctgga
     6421 aggattccag gaacgcgcgc ttggccgtcg tcgacatgtc gaggccgtgc gtgacgccgt
     6481 cccgctccag gtagtcgatc agggcgcgtt tcaggacggg gttgttgtgg ccgtagttga
     6541 gtgaaccggc tccggcgaag aagtcgaggt actcgtggcc gtcctcgtcg tacatgcggc
     6601 tgccgtgcgc acggtcgaag acggtgggcc agccgcggca gtagctgcgc acctcggact
     6661 cgagggtctc gaagacgctg aggtcgggct gggtgatggt cacgacgaat cgctcctcgg
     6721 tggcgtggga agtcagaggg agtcggaaga tcggggggtc ggccctgcgc gtcagagcgc
     6781 gaagggcccg atgcggtgca ggacttcggg gtcgtggggt ccgtcgggga agtccggggt
     6841 gtcgaagagc acctcgcgct ccagcagggc gccgtggcgc tccgcgaacg cggtgaacag
     6901 gcgctcggag gcggtgttgc cgggtgcgac ggtggtctcc acggtgtcca cgccgtgctc
     6961 cccggcggtc cgggcggcca gcgcgtcgag catggcggcg gccaggccgc ggccgcggtg
     7021 cgcctcgtcg acggcgacct gccagaccag cagggtgcgc gggctgtccg ggcggaggta
     7081 gccggtgatg aagccgaccg cctctccgcg ctcgtcgcga gcgacggccg aggtggcggc
     7141 gaagtcacgg caccacagca gatagctgta cgacgagttc aggtcgagga ccttcgagtc
     7201 cctggctatc cgccacagca cggctccgtc cgccacggtc gggcggtcga tttgcaggtc
     7261 tgcttgtgcg gcagtcatgc gaattgaact taccgaggta aattcaaaat tgcatcgtcc
     7321 tgcggggtta cgtacgagcg cgtcatgtgt tatcgcgcgt acgggtgtgt ccgcgcgaga
     7381 cctcggtgag atgtcccatt ttgcacggga ggaagtggtg caaatggggc gcgctgtgga
     7441 accggtcaca tctgtgtaac tctcgtgacc acacccggaa acgcctggcg gccgctcctc
     7501 ggattggggc gtttaggctc cggaagaccg ggcagaagaa gacgggaagc tgctctgcag
     7561 agtaataggg aaataagccg gggaatgtcg cgggagtatg tgtgcgaggg atttcggtaa
     7621 tcccttggaa ttcacgcccc ggtcaccccg tcgacgcgct cccggagaag atcggcgtgg
     7681 ccgttgtggc gcgcgtactc ctcgatcaca tggatcagca cccagcgcgg gctgacctcc
     7741 gtgccggccg gcggcccgtc ggcgatgcgt ccggtgccgt ccagggggcg cccggcgcac
     7801 aggtcccgtc cccaggcctg cgcggcaccc cccgccccgc atcggcaccg gtgcccaaga
     7861 agctcgtccg gggccgtggg gccccgcctc gctcacaccc aggcccgctc cgccgccgcc
     7921 aaccccgcgg gcaggtcgac cgacaccccc gcgtccgtca gggccgtagc cagagccgcc
     7981 aagcacgccc ggaccgtgcc acgggtggcc tcgggcccgt agtgattgac gcggatcatc
     8041 tcgcgggcca gggcgccgcc ccctgcggcg agaggcaggc cggggtcggc cgccaacgcc
     8101 cgcgcgacca gatccgacgc ggtcgtaccg gccggcgccc gcagcgtcgt ggcgaccggc
     8161 gccgcgtccg ccgcgtcgtg gacgtacggc gcgatccccc cgccgagcgc cgtcgcaccc
     8221 gcacgggtcg cggcggcagc cgaggcgtgc cgcgccatca ccgcctccgg tccctccgcc
     8281 tcgatccgct ccacgcacgc ctcgagcgcg agcatctcga gctgcgccgg agcgtgcagc
     8341 agcgtcttgc ggccaccgtc gatccaccgc tccttccagt cgaggaggga gaggtaggag
     8401 cggcgcggcg cgttcgggtt ggccgccatg cgggcccagg cacgctcgct caccgagacc
     8461 gccgacaccc cggcgggccc gcccatcgcc ttctgcgccc cgatgacgca caggtccacg
     8521 ccccacgcgt ccggcagcac cggctccgcg cccaccgagg cgaccgcgtc caggtagaac
     8581 agcgccccgt gctcccgcac gacctcgccg atcgcggcga ccgggttggt gttgccggtg
     8641 gcggcctcgg cgtgcaccag cgacacgaag tcgatctccg ggtgctcggc gaacgcgtcc
     8701 cgcacctgct cggcggtcac cgccgtgtgg aacggcacgg tcaggtcgat gacggtggct
     8761 ccgcagtccc gcagccagtc gccgaaggtc tgcccgtagg ggccggtgat gacgttcagc
     8821 gcggtcgtcc cgggcccggc cgccgcacgg atcgcgccct ccagcggcag cagcgcctca
     8881 ccctgggtga tcagcacgtc ctgcccggtg cccagcagcc gcgccacccg gtcctcgatc
     8941 gaggcgaagc gcgcggcgct cagcgggggc aggtccagga ggaggttcgt cacggctgtg
     9001 ctctctttgc tcatgcttca cggcagcgcg ggtaggtacg acgacgtcga gggtaaacct
     9061 tcggcggacg gttccgtttc ccccacagga ggcctccgtg aagacgcatc ccggccgcag
     9121 gacccgggtc ctggttgcca ccaccgcgac ggccgggatg gtgctcctcg ccgcctgcac
     9181 gtccaccgac gacggaggcg gagggtccac gaccgccggc ggggtcgagc tcgtcaaggc
     9241 ggggcagctc accacctgca cccacctgcc gtacccgccg ttccagtcgg agatcgacgg
     9301 cgaggtgcag ggcttcgacg tcgccctgat cgacctggtc gccgacgacc tcggcgtgaa
     9361 gcagcagatc ctcgacacgc cgttcgagaa cttcaagacc ggcgccttcc tcaactccgg
     9421 cgagtgcgac ctggccgcgg ccggcatgac catcaccgac gagcggaaga agaacgtcga
     9481 cttctccgac ccgtacttca acgccaccca ggccgtcctc gtcgccaagg acagcggcgt
     9541 cggctccctc gccgacgtga agtcggagaa cgtcgagctg ggcgcccagg cacagaccac
     9601 cggcgaggac tacgtcaagg gcgagggctt cgacccggtc tccttcgagt cctccgacgc
     9661 cgtcctcaac ggcctgcgca ccggccaggt caaggccgtc gtcatcgact accccgtcgt
     9721 ccagggctgg ctgaaggaca aggccaacgc ggacgccttc accgtcgccg agcagatcga
     9781 caccggcgag cagtacggct tcaccgtgaa gaagggcaac aaggccctcc aggatgccgt
     9841 gaacaaggcg ctcgccgacg ccaaggccga cggcacgtac gagaagctgt acgagcagtg
     9901 gatcggcccg tacgacgagt ccgccgcctc cccggccgcc tcctgaccca tggccgacac
     9961 ggaggagacg ccgctccggc ccaggcggaa gggcctgacc cggcggcaga agcgcagcgt
    10021 gtcgcgcggc atccagtacg ccgtgttcgt cgcggccgtg accgccttcg gcgtcaccgc
    10081 ggactgggga cggctgcaga accagttcgc gcaggtcgag atcgccgagc ggatgttccc
    10141 cgaggtcatc acgctcgcgc tgaagaacac cgtcctctac acggtgaccg gcttcggcgt
    10201 cgggctcgcg ctcggcatgc tgatcgcgct gatgcggctg tcgtccgtcg ggccctaccg
    10261 ctggctggcc ggcatctaca tcgagatctt ccgtggcctg cccgccctgc tgatcttcat
    10321 cttcatcggc gtcgccgtcc cgctcgcctt ccccggcacg gagatcatcg gcgggacgta
    10381 cggcaaggtc gccctcgcg
//
