LOCUS       NZ_RAII01000001        11311 bp    DNA     linear   CON 15-MAY-2024
DEFINITION  Streptomyces sp. E1N211 s-211_S3_L001_R1_001, whole genome shotgun
            sequence.
ACCESSION   NZ_RAII01000001
VERSION     NZ_RAII01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. E1N211
  ORGANISM  Streptomyces sp. E1N211
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7318313)
  AUTHORS   Vicente,C.M., Thibessard,A., Lorenzi,J.N., Benhadj,M., Hotel,L.,
            Gacemi-Kirane,D., Lespinet,O., Leblond,P. and Aigle,B.
  TITLE     Comparative Genomics among Closely Related Streptomyces Strains
            Revealed Specialized Metabolite Biosynthetic Gene Cluster Diversity
  JOURNAL   Antibiotics (Basel) 7 (4), 86 (2018)
   PUBMED   30279346
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7318313)
  AUTHORS   Leblond,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2018) Dynamique des Genomes et Adaptation
            Microbienne UMR 1128, Universite de Lorraine - Institut National de
            la Recherche Agronomique, Faculte des Sciences et Technologies
            Boulevard des Aiguillettes BP 70239, Vandoeuvre-les-Nancy F-54506,
            France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics Workbench v. 6.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003671735.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 02:33:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,941
            CDSs (total)                      :: 6,866
            Genes (coding)                    :: 6,131
            CDSs (with protein)               :: 6,131
            Genes (RNA)                       :: 75
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 736
            CDSs (without protein)            :: 735
            Pseudo Genes (ambiguous residues) :: 0 of 736
            Pseudo Genes (frameshifted)       :: 138 of 736
            Pseudo Genes (incomplete)         :: 673 of 736
            Pseudo Genes (internal stop)      :: 17 of 736
            Pseudo Genes (multiple problems)  :: 87 of 736
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:49:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2093367
            Orig. end                         :: 2104678
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            RAII01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11311
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11311
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11311
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6311](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6311
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            202..921
                     /locus_tag="EBH12_RS09595"
     CDS             202..921
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006130509.1"
                     /locus_tag="EBH12_RS09595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_121719210.1"
                     /transl_table=11
                     /translation="MNDDRTTPAPDSPFRPDPGDRDAAPQFVLPLVVRIERGSPPARTD
                     ALETAARAVLVMLSDERSVGEGEWARAIRDWQDARIRKVVRRARGAEWRRAEALPGITV
                     TGKSAEVRVFPPVPLDGWPRDLARLQVSGTDLDDPDPVPPADPGVPTLWMNPELEMSAG
                     KAMAQAGHGAQLAWWELPDAAREAWHAAGFPLAVRSADPADWPALTGGGLPTVRDAGFT
                     EIAPGSCTVVADHPALR"
     gene            990..2585
                     /locus_tag="EBH12_RS09600"
     CDS             990..2585
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (116-293). E-value:
                     3.3e-16. Score: 51.6. Matches aSDomain:
                     nrpspksdomains_EBH12_RS09600_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006130508.1"
                     /locus_tag="EBH12_RS09600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_121740326.1"
                     /sec_met_domain="PF00561 (E-value: 4.9e-26, bitscore: 90.6,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPTRRTTAAALLSGAVLLSASLAACGPAGEGGAGGGGGEEDDRGR
                     IWAQSLNWKDCPAPSPLQGGGEAPEPLPDGTRWQCATMRAPLDWDRPGGETVDVALIRA
                     ASRADTADRIGSLLFNFGGPGESGVVTLPALAPDYEQLRSRYDLVSFDPRGAGASTGVT
                     CLDAEAAQAMTRQDDTPDDGDDEVTALLQARQRYADACEQESGRVLPHVGTEDAARDMD
                     LLRHLLGDDKLHYFGISYGTELGGVYAHLFPRHVGRAVLDGVVDPTLDQMEGALAQAGG
                     FQLAFDHFAQWCVDTGCSLGDTPEEITRLVMELEETLDDEPLSVDGERVFTGSQLIDAV
                     VQALYLETAWPALEVGLQLLAADEGRTLLGLADALSGRHAGGWSDQQDARTAITCADRS
                     DRYTVDDVLEQSAAFEEASPLFGDTMVWGALECTDWPVRGKAPHPDVSAPGAPPVLLVG
                     NTGDPATPYEGAARMAERLGKGVGVELTYEGEGHGAYDSGNSCVRKAVNAYLLDGTVPA
                     AGTVCEREPLQGKG"
     aSDomain        1338..1868
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EBH12_RS09600_Abhydrolase_1.1"
                     /evalue="3.30E-16"
                     /label="EBH12_RS09600_Abhydrolase_1.1"
                     /locus_tag="EBH12_RS09600"
                     /protein_end="293"
                     /protein_start="116"
                     /score="51.6"
                     /tool="antismash"
                     /translation="LLFNFGGPGESGVVTLPALAPDYEQLRSRYDLVSFDPRGAGASTG
                     VTCLDAEAAQAMTRQDDTPDDGDDEVTALLQARQRYADACEQESGRVLPHVGTEDAARD
                     MDLLRHLLGDDKLHYFGISYGTELGGVYAHLFPRHVGRAVLDGVVDPTLDQMEGALAQA
                     GGFQLAFDHFAQWC"
     misc_feature    2718..2733
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.58"
                     /tool="antismash"
     misc_feature    2742..2761
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 23.35"
                     /tool="antismash"
     misc_feature    2745..2759
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 26.9"
                     /tool="antismash"
     gene            2772..4835
                     /locus_tag="EBH12_RS09605"
     CDS             2772..4835
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523447.1"
                     /locus_tag="EBH12_RS09605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="neutral/alkaline ceramidase"
                     /protein_id="WP_114875090.1"
                     /transl_table=11
                     /translation="MTAERPPSRSRRTVLRAGAVTGLGLAGAHLISPGAAAAATGSGSG
                     YLVGRGISDATGEVAEVGMMGYGRFDQQAAGLHTRLRARAFVVAEQASGRRVLLIVADS
                     PMIFESVHRAVLRRLRQAYGDLYTARNVLVTATHTHAGPGGYSHHLLYNTTTFGFHEAT
                     FEAVADGLFEAARNAHEDLAPSGLLLSHGTLTGASVNRSKTAFDRNPKADRDHFPDGVD
                     TLTTLLRVERQGRTVGAVNWFPVHGTSMSGDNRLISADNKGYAAYHWEREVHGVDYLAD
                     GTPEFVSAFAQTNSGDMSPNLDLVPPTTPADFEATRTSGLRQYEAAAAQADTPGVRLSG
                     PVDSRLVYVDLSDVTVRPEFTGDGKTHRTSKPCVGAAMAAGSLEDGPAFPGFDEGDNPF
                     WDAISHSVVYTVSPELKQAQAPKDVFVPIGEMNRIYPWVQERVPVMLVRIGGLYLIGIP
                     GEVTVCAGLRLRRTVAGIVGADLDDVLVAGYSNSYFHYVTTPEEYDAQQYEGGSTLFGR
                     WQLPALQQTAAELAVSLRDGTDLPLGPEPPDLTGKALSLQPGVVLDAPPLFRKFGDVLV
                     PPRENYRTGERVEVVFAGAHPGNDLHRGDTYLRIERRDSAGAWRTVADDGDWATRFHWA
                     RDGIAASKVTITWDVPQGTPSGTYRVVYRGDAKSLGGKISSIEGVSTAFQVR"
     misc_feature    4888..4897
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            5001..6311
                     /locus_tag="EBH12_RS09610"
     CDS             5001..6311
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006130505.1"
                     /locus_tag="EBH12_RS09610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_121740327.1"
                     /sec_met_domain="DUF692 (E-value: 6.1e-97, bitscore: 322.7,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVRLGTGIGWRPEIADAVEAMPGVDWVEVVAENVCPGHLPASL
                     LRLRERGATVVPHGVSLGLGGAGRPDERRLTALAERAAALGSPLVTEHIAFVRAGGPLT
                     ASPGIEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLAVENIAALFSWPGEEMTEGQF
                     LYELADRTGVRLLIDVANLHTNHVNRGEDPAEALAALPLEAVAYVHVAGGFERDGVWHD
                     SHAHPVPRPVLDILTDLASRVSPPGVLLERDEDFPPTAELERELDAIHGAVEAGDRVRG
                     DGAPERVREPAAAGPVAGATRERLALAQTAVLSSLVAGTPVPEGFDRTRMRVQARALAA
                     KRADVVAKVAPELPEILGDGYRPAFLAYAQTRPMTGGYRHDAMAFVEHLLRPGRLDDPR
                     TRRALREWHRDRSGPRPRSHRPTTRLARATRRALLRR"
     misc_feature    complement(6430..6443)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.76"
                     /tool="antismash"
     gene            6437..7219
                     /locus_tag="EBH12_RS09615"
     CDS             6437..7219
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006130504.1"
                     /locus_tag="EBH12_RS09615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_121740328.1"
                     /transl_table=11
                     /translation="MFWVPLLLLAWAIAATAGLRLCRAAVREAAAAGDGAGERRGVTLY
                     EAAFLSGGPARVADLTLVAMARQRRLLLAHTGWATVVDPRGRDAMERTVIGAIGPEGQS
                     RIAPVRKAAATAEAVRVLAERLVRAGLAVPEGDGRTVAEALRRLRLAVPAVAGLAAVAL
                     LMPVPSQMPRPLVALWFALPLAFTLGCLAIARFELSPASPWASPAGQRLLASVRRAAAG
                     DTDDDRAYLTTVAVRGVHAVGEPQVRAAFTHRGPDPRW"
     gene            7330..8931
                     /locus_tag="EBH12_RS09620"
     CDS             7330..8931
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (115-488). E-value:
                     2.6e-22. Score: 71.6. Matches aSDomain:
                     nrpspksdomains_EBH12_RS09620_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006130503.1"
                     /locus_tag="EBH12_RS09620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_121740329.1"
                     /sec_met_domain="PF00561 (E-value: 7.6e-27, bitscore: 93.2,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTAAALVATGSLLLTTPAAAARTPAAAPAPAPGSPSASEARGTA
                     VAAARAASEGIGFGPCAEEQELPPTVECGTLSVPLDYARPDGERIRLTVSRSRATGKDP
                     ATGRAVPRQGSLVNNPGGPGVSGMYFPMLGVVPGWKRLAAAYDLVGYAPRGVGRSAPLS
                     CTDPTAFQQGPAPAPQHPSAAVKRERAARAAAYARGCAERSGDALRHYTSLNNARDLDV
                     LRAALGETRLTFLGSSYGTYIGALYATLFPSHVRRMVFDSAVNPAPERIWYRNNLAQSA
                     AFEDRWADFRAWAARHHDVYGLGDTARKVGDSYDVVRERLAREPAGGKAGPAQLHAAML
                     KAAYYDDHWPQRAHALSAYLKGDPAPLVEQAEEQPEAAAEAENTRAVYLAVECNDAPWP
                     TDFAVWDRDNTRLARRAPFETWDNVWTNLPCAYWPAPRQRPLDVRAGQDALPPTLVLAA
                     ERDAATPYDGALELHRRLAGSALVTERDAGSHGLAGGANACVNAHLTAYLLEGRVPGRR
                     ASCAPHPAPAPAAPAS"
     aSDomain        7675..8793
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EBH12_RS09620_Abhydrolase_1.1"
                     /evalue="2.60E-22"
                     /label="EBH12_RS09620_Abhydrolase_1.1"
                     /locus_tag="EBH12_RS09620"
                     /protein_end="488"
                     /protein_start="115"
                     /score="71.6"
                     /tool="antismash"
                     /translation="LVNNPGGPGVSGMYFPMLGVVPGWKRLAAAYDLVGYAPRGVGRSA
                     PLSCTDPTAFQQGPAPAPQHPSAAVKRERAARAAAYARGCAERSGDALRHYTSLNNARD
                     LDVLRAALGETRLTFLGSSYGTYIGALYATLFPSHVRRMVFDSAVNPAPERIWYRNNLA
                     QSAAFEDRWADFRAWAARHHDVYGLGDTARKVGDSYDVVRERLAREPAGGKAGPAQLHA
                     AMLKAAYYDDHWPQRAHALSAYLKGDPAPLVEQAEEQPEAAAEAENTRAVYLAVECNDA
                     PWPTDFAVWDRDNTRLARRAPFETWDNVWTNLPCAYWPAPRQRPLDVRAGQDALPPTLV
                     LAAERDAATPYDGALELHRRLAGSALVTERDAG"
     gene            complement(8954..9685)
                     /gene="hemQ"
                     /locus_tag="EBH12_RS09625"
     CDS             complement(8954..9685)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="EBH12_RS09625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_048456006.1"
                     /transl_table=11
                     /translation="MSDDATTPAPDRIPNKGKLAKDLNEVIRYTLWSVFKLKDVLPEDR
                     TGYADEVQELFDQLAAKDVTVRGTYDVSGLRADADLMIWWHAETADQLQEAYNLFRRTK
                     LGRALEPVWSNMALHRPAEFNRSHIPAFLADETPRDYVSVYPFVRSYDWYLLPDEDRRR
                     MLADHGKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRGSEARMHVR
                     EEVPFYTGRRKDVADLVAGLA"
     gene            complement(9691..11148)
                     /gene="hemG"
                     /locus_tag="EBH12_RS09630"
     CDS             complement(9691..11148)
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (MITE)
                     MITE0000068: Oxidation (60% identity)"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523453.1"
                     /locus_tag="EBH12_RS09630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_121740330.1"
                     /sec_met_domain="DAO (E-value: 2.7e-11, bitscore: 42.3,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGSHTGGGGHAVVVGAGIAGLAAAHRLLEHGARVTVLEASERVG
                     GKLLPGEIAGARVDLGAESLLARRPEAVGLARAVGLADRLQPPATASAALWTRGALRPM
                     PKGHVMGVPGTAAALAGVLSDEGLARIERDAELPPTEIGDDVAIGEYVAERFGREVVDR
                     LVEPLLGGVYAGDAYRISMRSAVPQLFEAARAHPTLTEAVRAVQAKTAAGRQTGPVFTG
                     VAGGVGTLPLAVADSLRSRGVRILTGTPVTGLSREPGEGWRLVAGGEVLHADAVVVAVP
                     APAAAGLLRGEAPGAAAELSAVEYASMALITLAYRRDEARLPEGSGFLVPPVEGRGIKA
                     STFASRKWGWIAEENPGLAVVRTSVGRHGETELLHRDDDELVELSRHDLKAATGLAAAP
                     VAARVTRWDDGLPQYPVGHHARVARVRAHLAGLSGLAVCGAAYDGVGVPACIASAYAAA
                     DQVQGDLRAVQQLTAHPVQSLHGGAGE"
ORIGIN
        1 cgaacgcgtc gatcaccagc cctcggtcga ggatcagctc cagcacgtcg tagagaccgc
       61 tggagcctcc tccgccacgc tgcggcaccg cctgagtcat cgcgcatcag tcccttcccg
      121 accctgatgc cccctccacg ccccttgatg tccgcgggtg ccgcccggca ccgcccccgt
      181 cccgcttggc acactgggcg ggtgaacgac gaccggacga cacccgcccc cgacagtccc
      241 ttccgacccg acccgggtga ccgcgacgcg gcgccgcagt tcgtgctgcc gctggtggtc
      301 cgtatcgagc gcggctcgcc gcccgcgcgg acggacgcgc tggagacggc ggcccgcgcg
      361 gtgctggtca tgctgagcga cgagcgttcc gtcggcgagg gtgagtgggc gcgggctatc
      421 cgcgactggc aggacgcccg catccgcaag gtggtgcgcc gggcacgcgg cgcggagtgg
      481 cgccgcgcgg aggcgctccc cgggatcacc gtgaccggga agtcggccga ggtgcgcgtc
      541 ttcccgccgg tccccctcga cggctggccc agggacctgg cccggctcca ggtgtccggc
      601 accgacctcg acgacccgga cccggtaccg cccgcggacc ccggcgtgcc cacgctgtgg
      661 atgaacccgg agctggagat gtccgcgggc aaggcgatgg cccaggccgg gcacggcgcc
      721 caactcgcgt ggtgggaact gccggacgcg gcccgcgagg cctggcacgc ggccggcttc
      781 ccgctcgccg tgcgcagcgc cgaccccgcc gactggcccg cgctgaccgg cggcggcctg
      841 cccacggtgc gcgacgcggg cttcacggag atcgcccccg gctcgtgcac cgtggtggcg
      901 gaccaccccg cgctgcgctg accccggggc gtcccccgga tgggttgcgc ggccgtgcgg
      961 ctgccgtggg cccgccggag gctgggccca tgccgacacg acgtacgaca gccgctgccc
     1021 tgctgtccgg cgccgtcctg ctctccgcct ccctggccgc ctgcggcccg gccggcgagg
     1081 gcggggccgg aggcggaggc ggcgaggagg acgaccgcgg acggatatgg gcgcagtccc
     1141 tgaactggaa ggactgcccc gcaccgtccc ccctgcaagg cggcggagag gcgcccgagc
     1201 cgttgccgga cggcacccgc tggcagtgcg ccaccatgcg cgcgcccctg gactgggaca
     1261 gacccggcgg tgagaccgtc gacgtcgccc tgatccgggc ggcgtcccgc gcggacaccg
     1321 cagaccgcat cggctcgctg ctgttcaact tcggcgggcc cggcgagtcc ggtgtggtca
     1381 ccctgcccgc cctcgccccc gactacgagc aactccggtc ccgctacgac ctggtgagct
     1441 tcgacccgcg cggggccggg gcgagcaccg gggtcacctg cctggacgcc gaggcggcgc
     1501 aggcgatgac ccggcaggac gacacccccg acgacggcga cgacgaggtg accgcgctgc
     1561 tccaggcgcg gcagcggtac gcggacgcct gcgaacagga gtcgggccgc gtgctgccgc
     1621 acgtgggcac cgaggacgcc gcccgcgaca tggacctgct gcgccatctg ctcggcgacg
     1681 acaagctgca ctacttcggg atctcctacg gcaccgaact cggcggcgtg tacgcccact
     1741 tgttcccccg gcacgtcggt cgtgccgtcc tggacggcgt cgtcgacccg acgctggacc
     1801 agatggaggg ggcgctcgcg caggcgggcg ggttccagct cgccttcgac cacttcgccc
     1861 agtggtgcgt ggacaccggc tgctccctcg gcgacacccc ggaggagatc acgcggctcg
     1921 tcatggagct ggaggagacg ctggacgacg agccgctgtc cgtcgacggg gagagggtgt
     1981 tcaccggctc ccagctgatc gacgccgtcg tccaggcgct ctacctggag accgcctggc
     2041 ccgcgctgga ggtgggcctg caactcctcg ccgccgacga gggccggacc ctgctcggcc
     2101 tcgcggacgc cctcagcggc cggcacgccg gcggctggag cgaccagcag gacgcccgga
     2161 ccgccatcac ctgcgccgac cgcagcgacc gctacaccgt ggacgacgtg ctggaacagt
     2221 ccgccgcgtt cgaggaggcg tccccgctct tcggcgacac catggtgtgg ggcgcgctgg
     2281 agtgcaccga ctggccggtg cggggcaagg ccccgcaccc ggacgtgagc gcgccgggcg
     2341 cgccgccggt cctgctcgtc ggcaacaccg gcgaccccgc gacaccgtac gagggcgccg
     2401 cgcggatggc ggagcggctc ggcaagggcg tcggggtgga actcacctac gagggcgagg
     2461 gccacggcgc gtacgactcc ggcaacagct gtgtgcggaa ggccgtgaac gcgtatctgc
     2521 tggacggcac ggtgccggcg gcggggaccg tgtgcgagcg ggagccgctc cagggcaagg
     2581 ggtgacgccg ccgccctgga gcgggagccg gcccggtggg tccgtgggaa cacggaccca
     2641 ccgggccggc cgcgtcatgc gcgcacgact cttgccgtgt tcatggcggc cggtgtacca
     2701 atgaggcgcg gaacttgaat gttgttcatg ttccgttcgc cggggggccg accgaccctg
     2761 aggagcagcg tatgaccgcg gaacgtcctc cctcgcgcag ccgccggacc gtcctgaggg
     2821 cgggtgccgt caccggcctc gggctcgcgg gtgcgcacct gatctcgccc ggcgccgctg
     2881 ccgccgccac gggaagcggc agcgggtatc tcgtcggccg gggcatctcc gacgccaccg
     2941 gtgaggtcgc cgaggtcggc atgatggggt acggccgctt cgaccagcag gccgcggggc
     3001 tccacacccg gctgcgggcg cgggcgttcg tcgtggccga gcaggcgagc gggcgccgtg
     3061 tgctgctgat cgtcgccgac tcccccatga tcttcgagag cgtgcaccgg gcggtgctgc
     3121 gacggctgag gcaggcgtac ggcgatctgt acaccgcgcg gaacgtcctc gtcaccgcca
     3181 cccacaccca cgccggcccc ggcggctact cgcaccatct cctctacaac accacgacgt
     3241 tcggcttcca cgaggcgacc ttcgaggcgg tggcggacgg cctgttcgag gcggcgcgca
     3301 acgcgcacga ggatctcgcc ccgtccggac tcctgctcag ccacggcacg ttgaccggcg
     3361 ccagcgtcaa ccgctcgaag accgccttcg accgcaaccc gaaggccgac cgcgaccact
     3421 tcccggacgg cgtggacacc ctcaccaccc tgctgcgggt ggagcggcag ggccgcacgg
     3481 tcggggcggt caactggttc ccggtgcacg gcaccagcat gtccggcgac aaccgcctga
     3541 tcagcgccga caacaagggc tacgcggcct accactggga gcgcgaggtc cacggcgtgg
     3601 attatctcgc cgacggcaca ccggagttcg tttcggcgtt cgcccagacc aacagcggcg
     3661 acatgtcgcc caacctggac ctggtgcctc ccaccacgcc cgccgacttc gaggccaccc
     3721 gcaccagcgg gctgcggcag tacgaggcgg ccgcggcgca ggcggacacg ccgggcgtgc
     3781 ggctgtccgg gccggtcgac tcgcggctgg tgtacgtgga cctctccgac gtcaccgtgc
     3841 gcccggagtt caccggcgac gggaagaccc accgcacctc caagccgtgc gtcggtgcgg
     3901 cgatggcggc gggcagcctg gaggacgggc ccgccttccc cggcttcgac gagggcgaca
     3961 accccttctg ggacgcgatc tcccactcgg tcgtctacac ggtctccccc gagctgaagc
     4021 aggcgcaggc gccgaaggac gtcttcgtgc cgatcgggga gatgaaccgg atctacccgt
     4081 gggtgcagga gcgggtgccg gtgatgctgg tgcgcatcgg cggcctgtac ctcatcggca
     4141 tccccggcga ggtcaccgtc tgcgcgggcc tgcggctgcg gcgcacggtc gccgggatcg
     4201 tcggcgcgga cctcgacgac gtcctggtcg ccgggtacag caactcctac ttccactacg
     4261 tgaccacgcc ggaggagtac gacgcccagc agtacgaggg cggcagcacc ctgttcgggc
     4321 gctggcagct tcccgcgctc cagcagaccg cggccgaact ggcggtctcc ctgcgcgacg
     4381 gcacggacct gcccctcggg cccgagccgc cggacctgac cggcaaggcg ctgtcgctcc
     4441 agcccggtgt ggtgctggac gcgccgcccc tcttcaggaa gttcggcgac gtgctggtgc
     4501 cgccgcggga gaactaccgg acgggggaac gcgtcgaggt cgtcttcgcc ggcgcccacc
     4561 cgggcaacga cctgcaccgg ggcgacacct acctgcggat cgagcggcgg gactccgccg
     4621 gcgcgtggcg caccgtcgcc gacgacggcg actgggcgac ccgcttccac tgggcccgcg
     4681 acggcatcgc cgcgtcgaag gtgaccatca cctgggacgt cccccagggc accccctcgg
     4741 gcacctaccg cgtcgtctac cgcggcgacg cgaagagcct gggcgggaag atctcgtcga
     4801 tcgagggcgt gtcgacggcc ttccaggtgc ggtgagcccg gccgaggcgt tccccgacgg
     4861 tcctccccca acctcaaatg ttcctctgaa ggcggcccgg agacggttcg gtcgccgggc
     4921 gccgggtcat atccccggca cgtccggtgc ggacggatcg ggggccgggc gggaacgccg
     4981 gggtgaagga ggggacgggg atgacgacgg tgcggctggg gaccgggatc ggatggcgtc
     5041 cggagatcgc ggacgcggtg gaggcgatgc cgggcgtcga ctgggtcgag gtggtggcgg
     5101 agaacgtctg ccccggccat ctgcccgcgt cgctgctgcg gctgcgcgag cgcggcgcga
     5161 ccgtcgtgcc gcacggtgtg tccctggggc tcggcggcgc cggacgcccc gacgagcggc
     5221 ggctcaccgc gctcgccgag cgggccgcgg cgctgggctc gcccctggtc accgagcaca
     5281 tcgcgttcgt gcgggcgggc ggcccgctca ccgcgtcccc cggcatcgag gccgggcacc
     5341 tgctgccggt gccgcgcacc cgcgacgccc tggacgtgct gtgcgagaac gtccgcatcg
     5401 cccaggacgc gcttcccgtg ccgctcgccg tggagaacat cgccgcgctg ttctcctggc
     5461 ccggcgagga gatgaccgag ggacagttcc tgtacgagct ggccgaccgc accggcgtac
     5521 ggctgctcat cgacgtcgcc aacctgcaca ccaaccacgt caaccgcggc gaggacccgg
     5581 ccgaggcgct cgccgcactg cccctggagg ccgtcgcgta cgtccatgtc gcgggcgggt
     5641 tcgagcggga cggcgtgtgg cacgacagcc acgcccaccc ggtgccgcgg ccggtcctcg
     5701 acatcctcac ggacctcgcc tcccgcgtct cgccgccggg cgtgctgctg gaacgggacg
     5761 aggacttccc gcccacggcg gagctggagc gcgaactgga cgcgatccac ggggcggtgg
     5821 aggcgggcga ccgggtccgc ggggacgggg cgccggagcg cgtgcgggag ccggccgccg
     5881 ccgggcccgt ggccggggcc acccgtgagc ggctggcgct ggcccagacc gcggtgctgt
     5941 cctcgctggt cgcggggacg ccggtgcccg aagggttcga ccggacgcgg atgcgggtgc
     6001 aggctcgggc gctggccgcc aagcgggcgg acgtcgtcgc gaaggtggcc ccggagctgc
     6061 cggagatcct cggcgacggc taccggccgg ccttcctcgc gtacgcgcag acccgcccga
     6121 tgacgggcgg ctaccggcat gacgcgatgg ccttcgtgga acacctcctg cgccccggcc
     6181 gtctcgacga cccccgcacg cgccgcgccc tgcgcgagtg gcaccgcgac cgctcgggcc
     6241 cccgcccccg ctcccaccgt ccgacgaccc gcctcgcccg cgccacccgc cgggcactgc
     6301 tgcgccgctg acgccctccg ggacgccggc cggggacgac gtccaccccg accggaacgt
     6361 cacatacccg caccaccgtg tccggatcgt gaacaaggca tggtgttcct gcgaccgcgt
     6421 cccttacaaa caactcatgt tctgggttcc tctcctgctg ctcgcctggg cgatcgccgc
     6481 gaccgccggg ttgcggctgt gccgggccgc ggtgcgtgag gcggcggccg ccggtgacgg
     6541 tgcgggagag cggcgggggg tcacgctgta cgaggcggcg ttcctgtccg gcgggcccgc
     6601 acgggtcgcc gatctcacgc tcgtcgcgat ggcccgtcag cggcggctgc tgctggcgca
     6661 caccggctgg gccaccgtcg tcgatccgcg cgggcgggac gccatggagc gcacggtgat
     6721 aggggcgatc ggaccggagg gccagtcgcg catagcgccg gtgcggaaag ccgcggcgac
     6781 cgcggaggcg gtgcgcgtgc tggcggaacg gctggtgcgc gccgggctcg cggtgccgga
     6841 gggcgacggg cggaccgtcg cggaggcgct gcgccggctg cggctcgccg taccggcggt
     6901 cgcgggtctc gccgccgtcg cgctgctcat gccggtgccg tcacagatgc cgcgcccgct
     6961 ggtcgccctg tggttcgcgc tgccgctggc gttcacgctg ggctgtctcg ccatcgcccg
     7021 cttcgagctg agccctgcct cgccctgggc gtccccggcg ggtcagcgtc tgctcgcctc
     7081 cgtgcggcgg gcggcggccg gcgacaccga cgacgaccgc gcgtacctga ccaccgtcgc
     7141 cgtgcgcggg gtgcacgcag tcggcgaacc gcaggtgcgg gcggccttca cgcaccgggg
     7201 gcccgaccct cggtggtgac cgggccgtga accggtcacc gacacccggg gagcggtcct
     7261 tgcccggcac cacgcgtacc gaaaacatcc cttttgtcgc cgcagcgcac cgaagggatc
     7321 gcctcaccga tgagaaccgc cgccgccctc gtggccaccg ggtccttgct cctcaccacc
     7381 ccggcggccg ccgcccggac ccccgcggcg gcccccgcgc cggcccccgg gtccccgtcc
     7441 gcgtccgagg cgcgcggcac cgcggtggcc gccgcgcggg ccgcctccga gggcatcggc
     7501 ttcggcccgt gcgccgagga gcaggagctg ccgcccaccg tggagtgcgg cacgctgagc
     7561 gtcccgctcg actacgcccg cccggacgga gagcgcatcc ggctcaccgt gagccgctcc
     7621 cgggccaccg ggaaggaccc cgcgaccgga cgcgcggtgc cccgtcaggg ctccctggtg
     7681 aacaacccgg gcgggccggg cgtcagcggc atgtacttcc cgatgctcgg cgtggtcccg
     7741 ggctggaagc gtctggcggc ggcgtacgac ctggtcggct acgcgccccg cggggtgggc
     7801 cgctccgccc cgctctcgtg caccgacccg accgccttcc agcagggccc cgcgcccgcg
     7861 ccccagcacc cgtcggcggc ggtcaagcgg gagcgcgcgg cccgcgcggc ggcgtacgcg
     7921 cgggggtgcg ccgagcggtc gggcgacgcg ctgcggcact acacatcgct gaacaacgcc
     7981 cgtgacctgg acgtgctgcg cgccgcgctg ggcgagaccc ggctgacgtt cctcggctcg
     8041 tcgtacggca cgtacatcgg ggcgctgtac gcgacgctgt tcccctcgca cgtgcggcgg
     8101 atggtgttcg actcggccgt gaacccggcg ccggagcgga tctggtaccg caacaacctc
     8161 gcccagtcgg ccgcgttcga ggaccgctgg gcggacttcc gggcgtgggc cgcccgccac
     8221 cacgacgtgt acgggctggg cgacaccgcg cggaaggtgg gcgacagcta cgacgtcgtg
     8281 cgggagcggc tggcgcggga gccggcgggc gggaaggccg gtccggcgca gctgcacgcg
     8341 gcgatgctga aggcggcgta ctacgacgac cactggccgc agcgcgcaca tgctttgtcg
     8401 gcgtatctga agggcgaccc cgcaccgctg gtggagcagg cggaggagca gcccgaggcg
     8461 gcggcggagg cggagaacac gcgcgcggtg tacctggccg tggagtgcaa cgacgcgccc
     8521 tggccgacgg acttcgcggt gtgggaccgg gacaacaccc ggctggcgcg ccgggcgccg
     8581 ttcgagacgt gggacaacgt gtggaccaac ctgccctgcg cctactggcc ggcgccccgg
     8641 cagcggccgc tggacgtgcg cgccgggcag gacgccctgc cgcccacgct cgtcctggcg
     8701 gccgaaaggg acgcggccac cccgtacgac ggggcgctgg agctgcatcg gcggctggcc
     8761 gggtcggcgc tggtgacgga gcgggacgcc ggcagccacg gactcgcggg cggtgccaac
     8821 gcgtgtgtga acgcgcacct gacggcgtat ctgctggagg gccgggtgcc ggggcggcgc
     8881 gcgtcctgcg cgccgcaccc ggcgcccgct ccggcggctc ccgcgtcctg accggcgtca
     8941 ggccggaccg gcgtcaggcc agaccggcga ccaggtccgc gacgtccttg cggcggcccg
     9001 tgtagaacgg gacctcctca cggacgtgca tacgggcctc cgagccgcgc aggtgacgca
     9061 tcaggtcgac gatgcggtac agctcgtcgg cctcgaaggc gaggatccac tcgtagtcgc
     9121 cgagggagaa ggaggcgacc gtgttggcgc gcacgtccgg gtaaccgcgg gccatcttgc
     9181 cgtggtccgc gagcatccgg cggcggtcct cgtccggcag caggtaccag tcgtaggagc
     9241 gcacgaacgg gtagacgctc acgtagtcgc ggggcgtctc gtcggcgagg aacgccggga
     9301 tgtgcgagcg gttgaactcg gcggggcggt gcagcgccat gttcgaccag acgggctcca
     9361 gcgcgcggcc cagcttggtg cggcggaaga ggttgtaggc ctcctggagc tggtcggcgg
     9421 tctccgcgtg ccaccagatc atgaggtcgg cgtccgcgcg caggcccgac acgtcgtagg
     9481 tgccgcggac cgtgacgtcc ttggcggcga gctggtcgaa cagctcctgg acctcgtcgg
     9541 cgtagccggt gcggtcctcc ggcaggacgt ccttcagctt gaacacggac cacagggtgt
     9601 agcggatgac ctcgttgagg tccttggcca gcttgccctt gttggggatc cggtcgggcg
     9661 cgggggtggt ggcgtcgtca ctcatgtccc ttattctccc gctcctccgt ggaggctttg
     9721 caccgggtgc gcggtgagct gctgcacagc gcgcaggtca ccctggacct ggtcggcggc
     9781 ggcgtacgcg ctcgcgatgc acgcggggac gccgacgccg tcgtacgcgg cgccgcacac
     9841 ggccagcccg gacaggccgg cgagatgggc gcggacgcgg gcgacgcgcg cgtggtggcc
     9901 gaccgggtac tgcggcaggc cgtcgtccca gcgggtcacg cgggcggcga ccggggcggc
     9961 ggccaggccg gtggccgcct tcaggtcgtg ccgggacagc tccaccagct cgtcgtcgtc
    10021 gcggtgcagg agctccgtct cgccgtggcg gcccacggag gtgcgcacga cggccaggcc
    10081 ggggttctcc tcggcgatcc agccccactt gcgggaggcg aacgtggacg ccttgatgcc
    10141 gcggccctcg acgggcggca ccaggaaccc gctgccctcg ggcagccgcg cctcgtcgcg
    10201 ccggtaggcg agggtgatca gcgccatcga ggcgtactcg acggcggaca gctcggcggc
    10261 ggcgcccggc gcctcgccgc gcagcaggcc cgcggcggcc ggggcgggca cagcgacgac
    10321 gaccgcgtcg gcgtgcagca cctcgccgcc ggcgacgagc cgccagccct cgccgggttc
    10381 gcggctcagt ccggtgaccg gggtgccggt gaggatgcgg acgccccggg agcgcaggga
    10441 gtcggcgacc gcgagcggca gggtgcccac gccgcccgcg acgcccgtga agacggggcc
    10501 ggtctgccgg ccggcggcgg tcttcgcctg cacggcgcgg accgcctcgg tcagggtggg
    10561 gtgcgcgcgg gcggcctcga agagctgcgg gacggccgag cgcatggaga tgcggtacgc
    10621 gtcccccgcg tacaccccgc cgagcagggg ctccacgagc cggtcgacga cctcacggcc
    10681 gaaccgctcg gcgacgtact caccgatggc gacgtcgtcg ccgatctcgg tgggcggcag
    10741 ctcggcgtcc cgctcgatcc gggccaggcc ctcgtcggac agcacgccgg cgagcgcggc
    10801 ggcggtgccg gggacgccca tgacgtgccc cttcggcatg gggcgcaggg cgcctcgggt
    10861 ccacagcgcg gcggaggcgg tggcgggcgg ctggagccgg tcggcgaggc ccaccgcgcg
    10921 ggcgagtccg accgcctcgg gccggcgggc cagcaacgac tccgcgccga ggtcgacgcg
    10981 ggcgcccgcg atctcgccgg gcagcagctt gccgccgacc cgttccgagg cctccagcac
    11041 ggtgacccgc gccccgtgct ccagcagccg gtgcgcggcg gccagcccgg cgatgcccgc
    11101 tccgacgacg acggcgtgcc cgccgccgcc cgtgtgtgat ccgctcatgc tctccacctt
    11161 ctcaaacgcc gcgcgccggt ccgccagggc cgtgtggccc cgtcgagtcc ctaccgtgac
    11221 cccatcgcga ccgtgcgggc cgaacgcccg ctcccctcac ggcgtctgag gagcgtcagc
    11281 cgtcaggaca cgagggggag ccccgcatgc g
//
