LOCUS       NZ_RAII01000001        10216 bp    DNA     linear   CON 15-MAY-2024
DEFINITION  Streptomyces sp. E1N211 s-211_S3_L001_R1_001, whole genome shotgun
            sequence.
ACCESSION   NZ_RAII01000001
VERSION     NZ_RAII01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. E1N211
  ORGANISM  Streptomyces sp. E1N211
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7318313)
  AUTHORS   Vicente,C.M., Thibessard,A., Lorenzi,J.N., Benhadj,M., Hotel,L.,
            Gacemi-Kirane,D., Lespinet,O., Leblond,P. and Aigle,B.
  TITLE     Comparative Genomics among Closely Related Streptomyces Strains
            Revealed Specialized Metabolite Biosynthetic Gene Cluster Diversity
  JOURNAL   Antibiotics (Basel) 7 (4), 86 (2018)
   PUBMED   30279346
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7318313)
  AUTHORS   Leblond,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2018) Dynamique des Genomes et Adaptation
            Microbienne UMR 1128, Universite de Lorraine - Institut National de
            la Recherche Agronomique, Faculte des Sciences et Technologies
            Boulevard des Aiguillettes BP 70239, Vandoeuvre-les-Nancy F-54506,
            France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics Workbench v. 6.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003671735.1-RS_2024_05_15
            Annotation Date                   :: 05/15/2024 02:33:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,941
            CDSs (total)                      :: 6,866
            Genes (coding)                    :: 6,131
            CDSs (with protein)               :: 6,131
            Genes (RNA)                       :: 75
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 736
            CDSs (without protein)            :: 735
            Pseudo Genes (ambiguous residues) :: 0 of 736
            Pseudo Genes (frameshifted)       :: 138 of 736
            Pseudo Genes (incomplete)         :: 673 of 736
            Pseudo Genes (internal stop)      :: 17 of 736
            Pseudo Genes (multiple problems)  :: 87 of 736
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:49:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1046566
            Orig. end                         :: 1056782
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            RAII01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5216](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5216
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    394..403
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            849..2990
                     /locus_tag="EBH12_RS04750"
     CDS             849..2990
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1054: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010040511.1"
                     /locus_tag="EBH12_RS04750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="WP_196421208.1"
                     /transl_table=11
                     /translation="MVGVSEGQASARSHAPVGRPLLSLTLAALMDEVHAHSGAVYLLSP
                     DQPVLEMAVMAGLPRAFAAPWERIGLSAPIPVSDAVRERRLVWVGGGEEMARHYPRIAM
                     VLPYPFSLAALPVATPAKTYGAVFVTWPGSHPPELSDREREHLTAACDRLAVRLERAAE
                     HSLSLAHEPDLLAAPVVGGVAGSLGTVEAARMVSRLPYGLCSLDLHGRVAFANAAASDL
                     LGVAPDRLPGAHLWAAVPWLNDPVYEDRYRAALFSQDVTSFVALRPPDTWLSFRLYPST
                     TGISMRISRARAVTEMNRAAPPQTGAGTDRLVTITQVLGLAGALTEAVGVQDVVQLVAD
                     EIVPAVGGQGLVVLGSRAGRLRVLGHRGYADPLVVERFDGLPLSEPTPGGQVLRTGVPA
                     FFESRGDLERLYPALQTHPSEFSAWAYLPLIASGRPVGTCVLSYGAPHRFATEERAVLT
                     SLAGLIAQALERAMLYDVKHRLAHGLQEALLPSTLPALEGIEAAARYLPATRGMEIGGD
                     FYDLVPTDGVAAAVIGDVQGHNVTAAGLMGQVRTAVRAYTAVGQAPDEVMRSTNRLLLD
                     LGSELFASCLYLRLDAERGRAVMARAGHPPPLLRRPDGRVRVLDLAGGPLLGIDGTAAY
                     PSTEVALPPGSLLVLYTDGLVESPGVDLEDALAELCGRLTELGDLRLDELADALMRHGP
                     HSADRLDDTAVLLLRSLPR"
     gene            complement(3076..4428)
                     /locus_tag="EBH12_RS04755"
     CDS             complement(3076..4428)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994899.1"
                     /locus_tag="EBH12_RS04755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="S1 family peptidase"
                     /protein_id="WP_048454902.1"
                     /transl_table=11
                     /translation="MSHKRIPKRKAAMAVGGVVALGAAAILLPNANASQDGASDGVAAA
                     PKTLKAGDASDLATRLAGTLGDAYAGAWYDGEDKQLVVNVVRGENTMALKVRSAGAEVR
                     WVDNSMTELEAGAGTLKSEATIPGTSWAVDPRTNKILVTADSTVTGDKWDQLESTVENL
                     GSGMATLKKSAGTFRTFASGGDAIFAGGARCSLGFNVTAGDGSPAFLTAGHCAAAGDQW
                     SDQQGGEPIATVDQALFPGAGDFALLTYDDPATEAPSEVNVGGQTVPIVQAAEATVGQQ
                     VFRMGSTTGLADGQVLGLNATVNYPEGTVTGLIQTDVCAEPGDSGGSLFTQDGLAIGLT
                     SGGSGDCTVGGETFFQPVTTALEAVGATLGDGGAGAGGQAGGQEQGAGQDAGDGEAGAD
                     GQAGAGDQAGAGDQADGQDAGTGQDGAGGQDGTGTGSGRHSGQDSGLSRTH"
     misc_feature    complement(4972..4985)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.44"
                     /tool="antismash"
     gene            5001..5216
                     /locus_tag="EBH12_RS04760"
     CDS             5001..5216
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128984.1"
                     /locus_tag="EBH12_RS04760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_019527181.1"
                     /sec_met_domain="strepbact (E-value: 2.6e-35, bitscore:
                     119.4, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSTPQVETVEIADAELDAVSGGLSVNAVNTLTGALDGIAPVSGL
                     VDTAVGTVEGVTGLNTAPVTNLVAGL"
     gene            5328..6134
                     /locus_tag="EBH12_RS04765"
     CDS             5328..6134
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="EBH12_RS04765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_048454903.1"
                     /transl_table=11
                     /translation="MQFRQQALAKLQSPEDLDLPVRFARPQGWLVLSVTVIAMAAASVW
                     ALTGSVTSTVQAPAILTYGQGSYILQSPVAGQVTAVLAREGERLPAGSPVLKVRTPEGD
                     EVVRTLQAGRITALAAGAGQIIATGADVAAVEKVSRAKAPLYATVYVPAQKAAGIPAHA
                     SVDLTVQSVPSQEYGVLRGEVTEVDRSAQSPRQIAAFLGDPQLGEEFTRDGRPVAVTVK
                     LKRSDSTESGYAWSSADGPPFELTSMTLATGSIRLSDRRPVDWLLP"
     gene            6131..8353
                     /locus_tag="EBH12_RS04770"
     CDS             6131..8353
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978106.1"
                     /locus_tag="EBH12_RS04770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_114875614.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 3.3e-38, bitscore:
                     129.2, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTAQETRGRRRAAPPRRKVPTSRVRTVRTPTVLQMEAVECGAAS
                     LAMVLGHYGRHVPLEELRIACGVSRDGSRASNLLKAARSYGLTAKGMQMDLAALADVTA
                     PAILFWEFNHYVVYDGMGRRLGRRGVYVNDPAKGRRFVPLEEFDGGFTGVVLVMEPGED
                     FTRGGRKPGVLGAMPARLRGTAGTMPAVVLTSLLLVAVGAAVPGLSRTYIDEFLIGQQT
                     SMLDVLFASMGACVLLTLLLTWLQQANLLRGRLISSTLSSARFLRHLLRLPVTFFSQRS
                     PADLVQRLQSNDAVAETLARDLSAAGVDAVVVVLYAVLLYTYDPQLTFVGIGVALLNIL
                     AMRIVIRLRATRTAKLRADTARLTNTAYTGLQLIETLKATGGEDGYFRKWAGQHATTLE
                     EQQRLGVPSAWLGIVAPTLATLNSALILWIGGMRAVEGHISVGLLVAFQALVARFTAPL
                     TRLNGVAGRIQDFAADVARLKDVENFRTDPLYGRPGAGESTRRLHGHVELQNLTFGYNP
                     LDKPLLTGFDLTVGPGQQVALVGGSGSGKSTVSRLISGLYTPWEGVIRIDGQRIEDIPR
                     GALAASVSFVDQDVFLFEGTVRDNVALWDPSIPDEAVVEALRDAALYDVIVRRPGGIHS
                     PVEQDGRNFSGGQRQRLEIARALVRRPSILVLDEVTSALDAETELMVMDTLRRRGCACV
                     VIAHRLSTVRDSDEIVVLDQGTVVERGRHEELVARGGAYAALVRER"
ORIGIN
        1 gaggctgaag ccgcccggcg gcaggtccgc gccctcggcg acgacgggcg gcgcggggaa
       61 ggcgacggcg tcggcgacga gggcgaggca cgcccggtcg tcggcgtcga acccgccggg
      121 ccgctcgctc accgcgagca ggcagccgcc gccgtcgtgg cgcacgggca gggcggcgag
      181 gtggaactcg cggcggggcg tgcggcgcga ctgggactgg gcggccagat ccctcgggcc
      241 gagatacacc gtgtgtcccc ggtgcgcctc ggcgaccggg gagcggccgg cgacggagta
      301 gcagtcgcgc accccgcaca gcgtgcgcgg caccccggcg gtctccgcca ggcacagcag
      361 ctcgccgtcc tcgccgaggc tgtagaagcc ggcgaaggcg gccccggcga agacgagggc
      421 ctgttccagg atgcggcgca gccggtcggt cgcatggggg tcggccgcga gcgcgctcag
      481 tgcgccttcg gcgcgcggcg ttctcgtgct gcgtcccgca gcgccctcac cgaccacgtt
      541 ggccattaca gcgctaatag gacacaagcg cagcccctgt gcgcgaacgg cacgcggcag
      601 ggccgcgccg gttctcacac ggggtacgcg cccggcgcgc ggtgcgcgcg cccgcgacgg
      661 tgcgtggccc gggccacccg gacgtccgcg gggctccgcc tcgggccccg cgcctcgaac
      721 gccggagggg ctgagctcgc cttaccgcga cgcaacgggg tatccgcaca gatgcgcgcg
      781 cccggggcgt cccgctcgcc gtgcgggacg ccctcccgca ggggagattg ggtgcggggt
      841 gcgaggaggt ggtcggcgtg tccgaaggac aggcgtccgc gcggtcgcac gcccccgtgg
      901 gccgtccgct gctgtcgctg acgctggccg cgctgatgga cgaggtgcac gcccactccg
      961 gcgccgtgta tctcctctcc cccgaccagc ccgtgctgga gatggccgtg atggcggggc
     1021 tgccccgcgc cttcgccgcg ccgtgggagc ggatcgggct gagcgcgccg atccccgtct
     1081 ccgacgcggt gcgtgaacgg cggctggtgt gggtgggcgg cggggaggag atggcccgtc
     1141 actatccccg gatcgccatg gtcctcccct accccttctc cctggccgcg ctcccggtgg
     1201 cgacgccggc gaagacgtac ggcgcggtct tcgtcacctg gccgggctcg caccctccgg
     1261 agctgtcgga ccgggagcgt gagcatctca ccgccgcctg cgatcggctc gcggtgcggc
     1321 tggagcgggc cgccgagcac agtctgagcc tcgcccacga gccggacctg ctggccgcgc
     1381 cggtggtggg aggggtggcc ggctcgctgg gcacggtgga ggcggcccgg atggtgtccc
     1441 ggctgccgta cgggctgtgc tcgctcgatc tgcacgggcg ggtcgcgttc gccaacgccg
     1501 ccgcgtccga cctgctcggc gtggcgccgg accggctgcc gggcgcgcat ctgtgggcgg
     1561 cggtgccgtg gctgaacgac ccggtgtacg aggaccggta ccgggcggcg ctgttcagcc
     1621 aggacgtcac gtcgttcgtg gcgctgcgtc cgcccgacac ctggctgtcc ttccggctgt
     1681 acccgagcac caccggtatc agcatgcgga tcagccgggc ccgcgcggtg acggagatga
     1741 accgcgcggc gccgcctcag acgggtgcgg gcacggaccg gctggtgacc atcacccagg
     1801 tgctcggtct ggccggggcg ctgaccgagg cggtcggggt gcaggacgtg gtgcagctgg
     1861 tcgcggacga gatcgtcccg gcggtgggcg gccaggggct ggtggtcctc ggctcccgcg
     1921 cgggccggct gcgcgtgctc gggcaccgcg ggtacgcgga cccgctggtg gtggagcggt
     1981 tcgacgggct gccgctgtcc gagccgacgc cgggcggtca ggtgctgcgc accggggtgc
     2041 ccgccttctt cgagtcgcgg ggggacctgg agcggctgta cccggcgctg cagacgcatc
     2101 cgtcggagtt ctcggcgtgg gcgtacctgc cgctgatcgc ctcgggccgc ccggtgggga
     2161 cgtgtgtgct gtcgtacggg gcgccgcacc ggttcgcgac ggaggagcgg gcggtgctga
     2221 cgagtctggc gggcctgatc gcccaggcgc tggagcgggc gatgctctac gacgtgaagc
     2281 accggctggc gcacgggctt caggaggcgc tgctgccgag caccctgccg gcgctggagg
     2341 ggatcgaggc cgccgcccgg tatctgcccg ccacccgggg catggagatc ggcggcgact
     2401 tctacgacct ggtgccgacc gacggggtgg cggcggcggt gatcggggac gtgcaggggc
     2461 acaacgtgac ggcggccggg ctgatgggcc aggtccgtac ggcggtgcgg gcgtacacgg
     2521 cggtggggca ggcgccggac gaggtgatgc gcagcaccaa ccggctgctg ctggacctgg
     2581 gctcggagct gttcgcgagc tgtctgtatc tgcggctcga cgcggagcgg gggcgggccg
     2641 tgatggcgcg ggcggggcat ccgccgccgc tgctgcgccg tcccgacggg cgggtgcggg
     2701 tcctcgacct ggccggcggg ccgctgctgg gcatcgacgg cacggcggcc tatccgtcga
     2761 cggaggtggc gctgccgccc ggttcgctgc tcgtcctgta cacggacggt ctggtggagt
     2821 cgccgggcgt cgacctggag gacgccctcg ccgagctgtg cggccggctc accgagctcg
     2881 gcgacctgcg gctggacgag ctggcggacg cgctgatgcg gcacggcccg cactcggcgg
     2941 accggctgga cgacacggcg gtgctgctgc tgaggtccct gccgcgctga ccggctgacc
     3001 ggccccgcgg cgcggaggcg ccccggcccg cacacggccg gggcgcctca ggcacatggt
     3061 tgtgccgcgc acgggtcagt gcgtccggga cagcccggag tcctgaccgg agtgacggcc
     3121 ggagcccgta ccggtcccgt cctgtccgcc cgcgccgtcc tgtccggtgc cggcgtcctg
     3181 gccgtccgcc tggtcaccgg cgccggcctg gtcaccggct cccgcctggc cgtccgcgcc
     3241 cgcctcaccg tcgccggcgt cctggcccgc gccctgctcc tggccgcccg cctggccgcc
     3301 ggcgcccgca ccgccgtcgc cgagggtcgc gccgacggcc tcgagggcgg tggtgacggg
     3361 ctggaagaac gtctcgccgc ccaccgtgca gtcgccgctg ccaccggagg tcagaccgat
     3421 ggccagaccg tcctgggtga agagggagcc gccgctgtcg ccgggctccg cgcacacgtc
     3481 ggtctggatg agcccggtga ccgtgccctc ggggtagttc acggtggcgt tcaggccgag
     3541 cacctggccg tcggcgaggc cggtggtact gcccatgcgg aacacctgct gtccgacggt
     3601 ggcctcggcg gcctggacga tcggcaccgt ctgaccgccg acgttcacct cgctcggggc
     3661 ctcggtggcc gggtcgtcgt aggtgaggag cgcgaagtcg cccgcgccgg ggaacagcgc
     3721 ctggtcgacc gtggcgatcg gttcgccgcc ctgctggtcg gaccactggt cgccggcggc
     3781 ggcgcagtgt ccggcggtga ggaacgccgg gctgccgtcg cccgcggtga cgttgaagcc
     3841 gagcgaacag cgcgccccgc ccgcgaagat ggcgtcgccg ccggacgcga aggtacggaa
     3901 ggtgcccgcg gacttcttga gggtggccat gccggagccg aggttctcga cggtggactc
     3961 cagctggtcc cacttgtcgc cggtgaccgt gctgtccgcg gtgacgagga tcttgttggt
     4021 gcgcgggtcg accgcccagg aggtgcccgg gatggtcgcc tccgacttca acgtccctgc
     4081 cccggcctcg agttcggtca tgctgttgtc gacccagcgg acctccgcgc ccgcgctgcg
     4141 gaccttgagg gccatggtgt tctcgccgcg caccacgttg acgacgagct gcttgtcctc
     4201 gccgtcgtac caggcgcccg cgtaggcgtc gccgagcgtt ccggcgagcc gggtggcgag
     4261 gtccgaggcg tcgcccgcct tcagtgtctt cggcgcggcc gcgacgccgt ccgacgcgcc
     4321 gtcctgggac gcgttggcgt tggggagcag gatcgcggcg gcgccgaggg ccaccacacc
     4381 tcccaccgcc atcgcggcct tgcgcttcgg gattcgcttg tgactcaaac ttctcgacct
     4441 cctgggggga ccggagtcgt gccgccgtcc gacggctgtt cggggcgcct ggacggtcgt
     4501 tggtacgcaa gggagttgag gggtgttcaa cgcggagcgg atcctcaccg tccgccaccg
     4561 gacagagacg ctcggtgtcc gatccccggt cgaatcccgg cttcggcgaa tctgcacagc
     4621 gaatgcccaa cccggtcacc tgacccgccg tccgtccaca ggccgcgcat ccgggacacg
     4681 cctttgcggc cggtgtgtcg ctttccggcg ccgggggcgc aagtggccgg atgcgtcgat
     4741 gccgcggccc atgtgaagaa gtcgccgccg agccgtgccc tcgtctgcac ggttgggtgc
     4801 ctcctgggcg caagttgcct ggtgggacgt gtgaggaatt ggaagcgccc ggttgccgcg
     4861 tgctttgatc catcgcaccg caaggccgtt ccgggctccc ggcgacgggc gctcggagcg
     4921 cccgcggtcg cggccttcat ctgtccccag aggggggccg ctcccccctt gtgaaagtcc
     4981 atacgaaggg aagttccatc atgaactcca ccccccaggt cgagaccgtc gagatcgccg
     5041 acgccgagct cgacgccgtc tccggcggtc tgtccgtcaa cgccgtgaac accctcaccg
     5101 gcgccctgga cggcatcgcc ccggtttccg gcctggtcga cacggccgtc ggcaccgtgg
     5161 agggtgtcac cggcctgaac acggccccgg tcaccaacct ggtcgccggt ctctgatcgc
     5221 gccctcgttc cgccgagtcc cggagccgta ccccggctcc gggactctgt ggtgcgtccg
     5281 cagtccttcc gtcacgtcgc acagttcacc caggtgaggg aagacccgtg caattccgcc
     5341 aacaggccct cgccaagctc cagtcgccgg aggatctcga cctcccggtg cgtttcgccc
     5401 gcccgcaggg ctggctggtg ctgtccgtca ccgtgatcgc gatggccgcc gcgtccgtgt
     5461 gggcgctgac cggctcggtc acctccacgg tccaggcgcc cgccatcctc acgtacgggc
     5521 agggcagtta catcctgcag agccccgtcg ccggtcaggt caccgccgtg ctcgcgcgcg
     5581 agggcgagcg gctgccggcc ggctcccccg tgctcaaggt ccgtaccccg gagggggacg
     5641 aggtcgtgcg cacccttcag gcgggccgga tcaccgcgct cgccgccggc gccgggcaga
     5701 tcatcgcgac cggcgcggac gtcgccgcgg tggagaaggt gagccgcgcg aaggccccgc
     5761 tgtacgcgac ggtgtacgtg cccgcgcaga aggccgccgg cattcccgcg cacgcctccg
     5821 tcgacctgac ggtgcagtcg gtgccgagcc aggagtacgg cgtgctgcgc ggcgaggtga
     5881 cggaggtgga ccgctccgcg cagtccccac gccagatcgc cgcgttcctc ggcgaccccc
     5941 agctcggcga ggagttcacc cgggacggca ggcccgtggc ggtcaccgtg aagctcaagc
     6001 gctccgacag cacggagagc ggctacgcgt ggtcctcggc cgacggcccg ccgttcgagc
     6061 tgacgtcgat gaccctggcg accggttcca tccggctgtc cgaccggcgt cccgtggatt
     6121 ggctgctgcc gtgagcaccg cacaggagac ccgcggcaga cgccgcgccg caccgccgcg
     6181 ccgcaaggtg cccacgtcca gggtcaggac ggtccgtaca cccaccgtgc tccagatgga
     6241 ggccgtggag tgcggcgccg cctcgctcgc gatggtgctg ggccactacg gccgtcacgt
     6301 cccgctggaa gagctgcgca tcgcgtgcgg cgtctcgcgg gacggctcgc gcgccagcaa
     6361 cctgctgaag gcggcccgca gttacgggct gaccgccaag ggcatgcaga tggatctggc
     6421 cgccctcgcc gatgtgacgg cgccggccat cctgttctgg gagttcaacc actacgtcgt
     6481 ctacgacggg atgggccgcc gcctcggccg tcgcggggtg tacgtcaacg accccgccaa
     6541 gggccgccgg ttcgtgccgc tggaggagtt cgacggcggc ttcaccggcg tggtgctggt
     6601 gatggaaccc ggcgaggact tcacccgggg cggccgcaag ccgggcgtgc tcggcgcgat
     6661 gccggcgcgg ctgcgcggta ccgcgggcac catgcccgcc gtggtgctga ccagtctgct
     6721 gctggtggcg gtgggcgcgg cggtgcccgg cctcagccgt acctacatcg acgagttcct
     6781 gatcgggcag cagacgtcga tgctggacgt gctgttcgcg tcgatgggcg cctgcgtcct
     6841 gctgacgctg ctgctgacct ggctccagca ggcgaacctg ctgcgcggcc ggctgatctc
     6901 gtccacgctg tccagcgcgc gtttcctgcg tcatctgctg cggctgccgg tgacgttctt
     6961 ctcccagcgc agccccgccg acctggtgca acgcctccag tccaacgacg cggtggccga
     7021 gacgctggcc cgtgacctgt cggcggcggg cgtcgacgcg gtcgtggtgg tgctgtacgc
     7081 ggtgctgctg tacacgtacg acccgcagct cacgttcgtc ggcatcgggg tggcactgct
     7141 gaacatcctg gcgatgcgga tcgtgatccg gctgcgcgcc acccgtacgg cgaagctgcg
     7201 cgcggacacg gcgcggctca ccaacaccgc gtacacgggg ctccagctga tcgagacgct
     7261 gaaggccacc ggcggcgagg acggctactt ccgcaagtgg gccgggcagc acgccaccac
     7321 gctggaggag cagcagcggc tcggggtgcc gagcgcgtgg ctgggcatcg tcgcgccgac
     7381 gctggccacg ctgaacagcg cgctgatcct gtggatcggc ggcatgcggg cggtcgaggg
     7441 acacatctcg gtcggtctgc tggtcgcctt ccaggcgctg gtcgcccggt tcacggcgcc
     7501 gctgacccgg ctgaacgggg tggcgggccg catccaggac ttcgccgccg acgtggcccg
     7561 gctgaaggac gtcgagaact tccggacgga cccgctgtac gggcgtccgg gcgccggtga
     7621 gtcgacccgg cggctgcacg gccatgtgga gctgcagaac ctgaccttcg gctacaaccc
     7681 gctggacaag ccgctgctga ccggcttcga cctgacggtc ggtccggggc agcaggtggc
     7741 gctggtgggc ggctcgggca gcggcaagtc gaccgtgtcg cggctgatct cggggctgta
     7801 caccccgtgg gagggcgtga tccggatcga cgggcaacgc atcgaggaca tcccgcgcgg
     7861 ggcgctggcc gcgtccgtct ccttcgtcga ccaggacgtg ttcctgttcg aggggacggt
     7921 gcgggacaac gtggcgctct gggacccgtc gatccccgac gaggcggtgg tcgaggcgct
     7981 gagggacgcg gcgctgtacg acgtgatcgt gcgccgtcca ggcggcatcc acagcccggt
     8041 cgagcaggac ggccggaact tctccggcgg tcagcgccag cgtctggaga tcgcgcgggc
     8101 gctggtgcgc cggccgagca tcctggtgct cgacgaggtg accagcgcgc tggacgccga
     8161 gacggagctg atggtgatgg acaccctgcg ccgccgcggc tgcgcgtgcg tggtgatcgc
     8221 gcaccggctg tccacggtcc gcgacagcga cgagatcgtc gtgctggacc agggcacggt
     8281 cgtggagcgc ggacggcacg aggagctggt ggcgcgcggc ggcgcgtacg cggcgctggt
     8341 cagggagcga tgagatgact gccgtgcagg aggggcgggg cggcgacgtc gtcctgggcg
     8401 cgctcggcgc gctgggcgcc ccgatcgact gctccgggtt cgggcggctg gacctggagg
     8461 ggccgcaggt gctgtggctg atcgcgtccg gcgccgtgga cctgttcgcg gtggacgcgg
     8521 agcagcaggg gcactggcac cacctgggcc gtctggaggc gggctcgctg gtgccgggcc
     8581 cggtggcggg tccggcgcac acgctggtgg cgcggccgct gcgggactgc gtggtgcacc
     8641 gcatcgcgct gcgcgagctg tatcaggagg cccacaccca gacctggtcc tacgacgggt
     8701 acggcaaccc gcagtacgtg ccgccgacga cgagcccgct ggagtacgcg ctcgcgctgg
     8761 gcgtgggccg cagcctcgcc gtgctgttcc aggcgccgat ggcgacggag cgggcggcgg
     8821 cgcccaccga cgacgacgtg ttctggatgc aggtgccccc gggcagcgtg cagtacggct
     8881 ccgtgtacgg cgcggaggcc gcggccgatc tgctgatgga tccgtcgctg tggcagagca
     8941 tggtcgacca gcagtaccgg ctgatgacca cgctggaccg gtggatcgag cagctggagg
     9001 agacgcacga gtcgcgcacc gcgcagggca tcgcggccgg cgaggcggtg cgggcgcacg
     9061 ccgaccggac gctgctcgcg tcgatcggca agcggtcggg gcagcgcacg acggccgcgg
     9121 acgcggacgc cacgtacgcg gcctgcgaac tggtggcgcg ggcggcgggg atcacgctgg
     9181 cctcgccggc ccggtccggc accggcgccg accggctgga tccggtggag caggtggcgc
     9241 tggcctcgcg gctgcgcacc cgggtggtgc gtcttgaggg ccggtggtgg cgcgacgacg
     9301 tgggaccgct ggtgggtcac cgcgccctgt cgggggcgcc ggtggcgctg ctgtggcggc
     9361 gcggcggcta tgtggccgtg catccgtcga cgggccggga gaccccggtg gagaaggcga
     9421 acgccgagga gttcgagccg cgcgcggtga tgttctaccg tccgctgccc gagcggcggc
     9481 tgagcccgct gcggctgatg cggttctgca tgggcggcac ccggggcgac ctgacggggc
     9541 tgctgctgag cggtctggtg acggtggtgc tcggggccct ggtgccgctg gcgacgggcc
     9601 gggggctcgg cgagtacgtg ccgaaggccc ggacggacct catcgtgcag gtgtgtctgg
     9661 cggtgatcct cagcagtgtg gtcgcggccg cgttcatgct gctgcagaac ctcacgctgc
     9721 tgcggctgga gggccgggtg gaggccacgc tccagccggc ggtgtgggac aggctgctcc
     9781 ggctgccgac ccggttcttc acggagcggt ccacgggtga gctggctagc gcggcgatgg
     9841 gcatcagcgc catccgccgg atgctggccg gtgtcgggcc cacggtgacc cagtcggtca
     9901 cggtgggcgc ggtgaacctg gcgctgctgc tgtggttcag cgtgccgatg gcgctggccg
     9961 cgatgggcat gctggtggtg atcgcgggcg tgttcctggg tctcgggctg tggcaggtgc
    10021 gctggcagcg acggctggtg gtgctcacca acaagctgaa caaccaggcg ttccagacgc
    10081 tgcgcgggct cccgaagctg cgcgtggcgg cggcggagaa ctacgcgtac gcggcctggg
    10141 cgtcgcagtt cgcgcgcagc cgtgagctcc agcagaaggc cgggcggatc aagaatctga
    10201 cggcggtgct gggcgc
//
