LOCUS       NZ_RCDD01000002        10795 bp    DNA     linear   CON 04-MAY-2024
DEFINITION  Actinokineospora cianjurensis strain DSM 45657 Ga0180969_102, whole
            genome shotgun sequence.
ACCESSION   NZ_RCDD01000002
VERSION     NZ_RCDD01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinokineospora cianjurensis
  ORGANISM  Actinokineospora cianjurensis
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Actinokineospora.
REFERENCE   1  (bases 1 to 1225445)
  AUTHORS   Goeker,M.
  TITLE     Genomic Encyclopedia of Archaeal and Bacterial Type Strains, Phase
            II (KMG-II): from individual species to whole genera
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1225445)
  AUTHORS   Goeker,M., Huntemann,M., Clum,A., Pillay,M., Palaniappan,K.,
            Varghese,N., Mikhailova,N., Stamatis,D., Reddy,T., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2018) Prokaryotic Super Group, DOE Joint Genome
            Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.9.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 102.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003663795.1-RS_2024_05_04
            Annotation Date                   :: 05/04/2024 02:08:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,669
            CDSs (total)                      :: 6,606
            Genes (coding)                    :: 6,552
            CDSs (with protein)               :: 6,552
            Genes (RNA)                       :: 63
            rRNAs                             :: 4, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 3, 1 (5S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 10 of 54
            Pseudo Genes (incomplete)         :: 50 of 54
            Pseudo Genes (internal stop)      :: 2 of 54
            Pseudo Genes (multiple problems)  :: 7 of 54
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:04:47
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 267865
            Orig. end                         :: 278660
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            RCDD01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10795
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="2"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10795
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10795
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5795](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5795)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    152..167
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 22.87"
                     /tool="antismash"
     misc_feature    152..168
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 22.36"
                     /tool="antismash"
     gene            313..573
                     /locus_tag="CLV68_RS15865"
                     /old_locus_tag="CLV68_3185"
     CDS             313..573
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CLV68_RS15865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CLV68_3185"
                     /product="hypothetical protein"
                     /protein_id="WP_121391462.1"
                     /transl_table=11
                     /translation="MASTATWAIIVTLGSANVTVLGAWLLKRRSGTSVEVEIERDGSGW
                     ERQTIKITTSAEEAPEAAVLRQLRAFTPPVLPADDPGAGTV"
     gene            557..772
                     /locus_tag="CLV68_RS33380"
                     /old_locus_tag="CLV68_3186"
     CDS             557..772
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CLV68_RS33380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CLV68_3186"
                     /product="hypothetical protein"
                     /protein_id="WP_147460014.1"
                     /transl_table=11
                     /translation="MPGRSEVRDFTEAPANHVVLSIERLPRLLDDDNVPPIEFLPPFLH
                     ESTHHWCFSPPSTRRSSCSTCAPGSN"
     misc_feature    1152..1166
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.33"
                     /tool="antismash"
     gene            1247..1498
                     /locus_tag="CLV68_RS15875"
                     /old_locus_tag="CLV68_3187"
     CDS             1247..1498
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CLV68_RS15875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CLV68_3187"
                     /product="hypothetical protein"
                     /protein_id="WP_121391466.1"
                     /transl_table=11
                     /translation="MATPDALVAFEKYIFEKIHRLPSACWRRTSGSSTPRGGGGGDLFA
                     TIGTTQVEAWETLKAVADQDGVFRVDKTAIEGILYCSV"
     gene            complement(1623..1832)
                     /locus_tag="CLV68_RS15880"
                     /old_locus_tag="CLV68_3188"
     CDS             complement(1623..1832)
                     /GO_function="GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /locus_tag="CLV68_RS15880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CLV68_3188"
                     /product="tautomerase family protein"
                     /protein_id="WP_121391467.1"
                     /transl_table=11
                     /translation="MPILSVTTWPTPDDKIRELVEALTTTMHRVTGAPLDKITVHVQEI
                     APDRWAEGGVLGTNPDFATLSRRQ"
     gene            complement(1832..2653)
                     /locus_tag="CLV68_RS15885"
                     /old_locus_tag="CLV68_3189"
     CDS             complement(1832..2653)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CLV68_RS15885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CLV68_3189"
                     /product="hypothetical protein"
                     /protein_id="WP_121391469.1"
                     /transl_table=11
                     /translation="MVALTPTTQGPLYPPSEIMNADGDFVVVGRVNRPAPDGGVVAPWE
                     SAIVSAKSPVPEFGGHLPHDVLREITPADDDMVLYTLPTPLPCNNYDMTFAPDQPPAAR
                     VLPSYPLHQVPIPDLRAEDGRKVTEPITLGRWLRAKGEVSVRVCPDGHHADFDFEFADL
                     IPNSLYTVMSLRELDLDPVAGPTRPGPLGIPNVFVTDRCGAARYSATLPDPFPTTDRPG
                     RNRIINVIVLWMSYQRSYGGAIGQYGLGGDIHAQLKLTVPSFHEYATTQEP"
     misc_feature    complement(2642..2644)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(2653..3258)
                     /locus_tag="CLV68_RS15890"
                     /old_locus_tag="CLV68_3190"
     CDS             complement(2653..3258)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CLV68_RS15890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CLV68_3190"
                     /product="hypothetical protein"
                     /protein_id="WP_121391471.1"
                     /transl_table=11
                     /translation="MAATTLLVTEVTTAADALDAAAKQWAEIRPEGHLFRSLEGSTLLR
                     LTPLSGVEALAGETELFEREFAELAPYVAGDFRRQVLTFVEAAKSTADAVPLTRYVQLR
                     HIEVPPGVFADYRKWREGTIFDVVRRAEEVEVFLAYHSLLSTEPGVMFVSGFDTTPERY
                     GAVFTSAEYQEIVRQAGSAYIAGGERGLFTTIYERVTG"
     gene            complement(3285..4331)
                     /locus_tag="CLV68_RS15895"
                     /old_locus_tag="CLV68_3191"
     CDS             complement(3285..4331)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) fabH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076296.1"
                     /locus_tag="CLV68_RS15895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CLV68_3191"
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="WP_246009899.1"
                     /sec_met_domain="fabH (E-value: 6.2e-86, bitscore: 284.4,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQDCFCGCALAEATDGPAVHQTPMPTSGIGILGCGAYLPEEEVG
                     NDVVAAEVGVETSWIERKTGILARRRAASWQAASDLAVPAAEEALERAGLAATDLSVII
                     IATSTPDSPQPATACVVQDKLGAVNAAAFDVNAVCSGFVYALETARRLVADGGHALVVG
                     VDVYSRILDPTDRRTAVLFGDGAGAVVVGPTRPGRGIVASSLSSYGQHAGLIEVAGGGS
                     RVPPTKESLAAGAHYFTMNGRAVREFVDREVPGAVRGFLASTGYADDTVAHFVPHQANG
                     HMLADLERTLAFPTARTHVTFERYGNTGAASVPITLAAAQHEIAEGDLVLLTAFGGGMA
                     MGMSLIRW"
     gene            complement(4328..4996)
                     /locus_tag="CLV68_RS15900"
                     /old_locus_tag="CLV68_3192"
     CDS             complement(4328..4996)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /locus_tag="CLV68_RS15900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CLV68_3192"
                     /product="HAD-IB family hydrolase"
                     /protein_id="WP_121391473.1"
                     /transl_table=11
                     /translation="MRVAAFFDIDETLITGKSMFDFLRFHLADDRSPRLGQAERFAAFE
                     QAAAGGGLPRAEANREYYRIYRGVPADLVDLQGREWFEQRRRTPGFINPRVVGALVRHR
                     LAGHRVVLVSGSFPACLRPLADALGAHHLLCTRPEVGADGLLTGAVDRSVIGAVKAVAM
                     REVAERFDLSLPLSFGYGDHVSDLPLLREVGHPVVVGADPELGRHAESNRWLRLAGVAA
                     "
     gene            complement(5001..5795)
                     /locus_tag="CLV68_RS15905"
                     /old_locus_tag="CLV68_3193"
     CDS             complement(5001..5795)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF015700.4"
                     /locus_tag="CLV68_RS15905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CLV68_3193"
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="WP_170224392.1"
                     /sec_met_domain="AfsA (E-value: 7e-16, bitscore: 54.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSASSGIDLASREDQVGATVVCLVGDRFAGMRAVPGVHTLSQLV
                     ADLRRGEPVPEAIVVGQGVGEYELDYLHSVLAKRGNANETVVLGIDGAPVARSLVHKHR
                     EQNVLLSDLRPVAAGHWVAGLVVHGENELLLDHQTGQHVQGMVVTEAMRQMFIAVFEAE
                     HGVRHPERHYYVVWNSIDLTFESFLFPLPARLTCEIVESAVQDPERMTFRVRMELAQSG
                     AVAAHADIRFAAFEDRRIKRSELRRATSAVESLVGRTTAKEC"
     misc_feature    complement(5766..5768)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(5988..7196)
                     /locus_tag="CLV68_RS15910"
                     /old_locus_tag="CLV68_3194"
     CDS             complement(5988..7196)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1184: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /locus_tag="CLV68_RS15910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CLV68_3194"
                     /product="MFS transporter"
                     /protein_id="WP_121391476.1"
                     /transl_table=11
                     /translation="MTAQPDTTRPPAAVEDTDPRSWPAVWVLGLGTFLFVTTELLPVGL
                     LPSIGAGVGVPVGIAGLVVSVYGGIAALTAAPLTSWVRRMDRRTLLVSLLGLLVLGNVI
                     SAVAPNYLVLMLGRVVIAFAHGVFWSTTPAVGTRLVEPRNAVRALSVVLGGISVASVVG
                     VPLGTAIGQATNWRVAFLAAAGAGLLVLVAVLVLLPSMQARNSGGLAAIPQLLRNGPFL
                     VAIAVAALTMIGHYLAYTYVTPYLEDVVGISPGMVSVLLLVFGAAGIAGNFLGGGAIAK
                     GLRRALVSSVALLAASLLVISLTGGSSTIAAVVLLVTWGLSYAALPVCLQTWVLRSAPE
                     ATDAASSVYVAVFNASIALGALGGGIAVESAGVGSVTAVGAVLVAAALIVLLVSRKARH
                     NEG"
     gene            complement(7193..7579)
                     /locus_tag="CLV68_RS15915"
                     /old_locus_tag="CLV68_3195"
     CDS             complement(7193..7579)
                     /GO_function="GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /locus_tag="CLV68_RS15915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CLV68_3195"
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="WP_121391478.1"
                     /transl_table=11
                     /translation="MSEREQVGREVMLGLGIDPDAVIASVAPLDDRFGTTIVEYAFGDI
                     VSRPGLDLRTRQLVTLALLAAQGGCGPQLDTHIPATLRAGATRAEIVALFVHLTPYVGI
                     PRSLNALYQAKELFAAADSQEATP"
     gene            complement(7576..8601)
                     /locus_tag="CLV68_RS15920"
                     /old_locus_tag="CLV68_3196"
     CDS             complement(7576..8601)
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072635.1"
                     /locus_tag="CLV68_RS15920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CLV68_3196"
                     /product="aldo/keto reductase"
                     /protein_id="WP_121391480.1"
                     /transl_table=11
                     /translation="MSETAQPPEAGRTRILGGDPVGLFGLGCMGMSEFYGAHDDARSTE
                     VLHAAIDAGVTLLDTADMYGGGHNERLVGAVLRTRSAFVATKFGIRRDGERRWNDSSPG
                     YLRTACDASLGRLGVDTIDLYYAHRLDGTTPVEDTVGALAELVQAGKVRHIGLSEVDAA
                     TLRRAHAVHPIAAVQSELSLWTRDVVTDGVLAAARELGTALVAYSPLGRGFLTGALRDR
                     DGLAEDDFRFTNPRFTETNLAANLPLLGAIDQVVERHGASHAQIALAWVLAQGVDVIPI
                     PGTRRLDHLRENLAAAAITLTDAEVAALDAAFAPDKVHGQRYPAATLPVTGPASTEQAR
                     A"
     gene            8749..9630
                     /locus_tag="CLV68_RS15925"
                     /old_locus_tag="CLV68_3197"
     CDS             8749..9630
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /locus_tag="CLV68_RS15925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CLV68_3197"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_121391482.1"
                     /transl_table=11
                     /translation="MAQQTDLSTFLRSRRAKVRPADIGLTNGHTVRRTQGLRREEVAAS
                     AGVSVDYYTRLEQGRERNPSIAVLESLATTLLLEGEERDHLFRLAAHTGNRAQATPPAP
                     RAVRPAVRRLLDTVTPSPAYVLNRWNDMLAANPAGLALLAGLDEWPPARRNTVRYIFLH
                     PTAPTLFEDWGSIAHNAVAHLHAMEGLLPGAPALQTLVDELSEQSQEFRALWQQHDVRS
                     LSTGRKLLNHPNVGRMALTYEVLDITNDHQRLVIYQAPVGTVDHDSMMLLNLVNPSAET
                     TAERPRRRSDQR"
     gene            complement(9959..10615)
                     /locus_tag="CLV68_RS15930"
                     /old_locus_tag="CLV68_3198"
     CDS             complement(9959..10615)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685523.1"
                     /locus_tag="CLV68_RS15930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CLV68_3198"
                     /product="response regulator transcription factor"
                     /protein_id="WP_121391484.1"
                     /transl_table=11
                     /translation="MTVRVLVADDQDLVRTGLSMILDARPDIEVVGQAADGHAAVELAH
                     RLRPDVCLVDIQMPGLDGIEVTRLLAGRGVPDPIAVVVITTFDLDEYVHGALRAGARGF
                     LLKDAGQDLLVQAVHAAAAGDALIAPNITRRLLATLAARDPSTRRTQPIEPLTEREEQV
                     LALVAGGRTNAEIAAELFIGLTTAKTHVASLLTKIGARNRVEIAMWAYDTGRAGT"
ORIGIN
        1 tgctgggggt gtggtggtac ccggcggcgc tcgcgggtgc gtgcgcggcg ctgatcgggt
       61 ggcgccggac ccggttcacc accaacgcca tcgcgacgtt gatggaggcc acagtggaca
      121 ctcacctgga cctgctgaca accgcgctgg gatacccctc gggcggactg gacggcgaga
      181 ccgcgccact gatcaacgac cggttcgaca agggttcctg aaagcgcgtc cggtgcccgc
      241 gcccgccagt atcgtcggac ggggcctggc tgtgccccga catcgccgcc gagcaggagc
      301 aaccgcgctc gggtggccag cacggcgacc tgggcgatca tcgtcaccct cggctcggcg
      361 aacgtcacgg tgctcggggc gtggctgctc aaacgccggt cgggtaccag cgtggaggtc
      421 gagatcgaac gcgacgggtc cggctgggaa cgccagacca tcaagatcac cacgtccgcc
      481 gaggaggcgc cggaggccgc tgtgctgcgg caactgcggg cgttcactcc cccagtgctg
      541 cccgccgatg acccgggtgc cgggacggtc tgaggtgcgc gacttcaccg aggcgcccgc
      601 caaccacgtc gtgctctcca tcgagcggtt gccgaggctg ttggacgacg acaacgtccc
      661 gcccatcgag ttcctgccac cgttcctgca cgagtcgacc caccactggt gtttctcgcc
      721 cccgtctaca cggcgctcgt cctgctcaac atgcgcgccc gggtcgaact gatgctggcc
      781 gtcgaggcca ccgacccggt cgaggtggcg aagtgggaag cgcgcgccac cgacgacctg
      841 tcgcggtcac tggtggccat ggaactgttg cgcccgttgg ccgaagggtt ggcgctgttc
      901 gccgagttcg acgtctcgcc gcccgccagc cgaccgttcc acccgccgcc gttgaagtgg
      961 gccgcgcggt tcctgggcgc ccaggaccgg aggatgccgt ggaccgagct cgccgggcac
     1021 cgcaccggcg acgacgtccg caggcgcaag gagaacctgc tggcgcaacc gctcgacaca
     1081 ggcgcgagtg acgggtacct cgcgggctac ctgctggtga agtcggtcat gcacgcgttc
     1141 cggatcagcg accacaagct gtggaccgag gacaggctgc tccaccaagt ggtaccgctg
     1201 gttcttcgag gacggggaga gggtcgaact cttcctcgcc accaacgtgg ccacgccgga
     1261 cgcactggtc gcgttcgaga agtacatctt cgagaagatc caccggctgc cgagcgcgtg
     1321 ttggcggcgg accagtggga gcagcacccc gcgcggtggc ggcggtgggg acctgttcgc
     1381 cacgatcggt acgacacagg tcgaggcgtg ggagacgctc aaggcggtgg cggatcagga
     1441 tggtgtgttc cgtgtggaca agaccgccat cgagggcatc ctgtactgct cggtctgacc
     1501 agtggcgtcg cacaacacca gcgcctgccc gtggtgggct cacgccgcgg ctggtcggca
     1561 gcgggcggga gcacagtgga gtggcccccg ccccccggac aaggggcggg ggccacagtc
     1621 ttctactggc ggcggctgag ggtggcgaag tcggggttgg tgcccaggac gccgccctcg
     1681 gcccagcggt cgggggcgat ttcctggacg tggacggtga tcttgtccag gggggcgccg
     1741 gtgacgcggt gcatggtggt ggtgagggct tccacgagtt cgcggatctt gtcgtccggg
     1801 gtcggccagg tggtgacgga caggatgggc atcagggctc ctgggtggtg gcgtactcgt
     1861 ggaaggacgg gacggtcagc ttgagctggg cgtggatgtc gccgcccagg ccgtactggc
     1921 cgatggcgcc gccgtagctg cgctggtagc tcatccacag cacgatgacg ttgatgatcc
     1981 ggttgcggcc cgggcggtcg gtggtcggga acgggtcggg cagggtggcc gagtaccggg
     2041 ccgcgccgca gcggtcggtg acgaacacgt tggggatgcc gagcggtccc ggcctggtcg
     2101 gcccggcgac cgggtcgagg tcgagttcgc gcagcgacat caccgtgtag aggctgttcg
     2161 ggatgaggtc ggcgaactcg aagtcgaagt cggcgtggtg gccgtccggg cagacccgca
     2221 ccgacacctc gcccttggcc cgcagccagc ggccgagggt gatcggttcg gtgaccttgc
     2281 ggccgtcctc ggcgcgcagg tccgggatgg gcacctggtg cagcgggtag ctgggcagga
     2341 cccgggccgc gggtggctgg tccggcgcga aggtcatgtc gtagttgttg cacggcagcg
     2401 gggtgggcag cgtgtacagc accatgtcgt cgtcggccgg ggtgatctcg cgcaggacgt
     2461 cgtgcggcag gtgcccgccg aactcgggca ccgggctctt cgcgctgacg atggccgact
     2521 cccacggggc gaccacgccg ccgtcgggtg ctggccggtt gacccgtccc accacgacga
     2581 agtcgccgtc ggcgttcatg atctcgctgg gcgggtacag cggcccctgg gtggtgggtg
     2641 ttaaggcgac catcagcccg tgacacgctc gtagatggtg gtgaacagcc cgcgctcgcc
     2701 accggcgatg taggccgacc cggcctgccg gacgatctcc tggtactcgg cgctggtgaa
     2761 cacggccccg taccgctcgg gggtggtgtc gaagccggag acgaacatga cgccgggctc
     2821 ggtgctcagc agcgagtggt atgccaggaa cacctcgacc tcctcggcgc ggcgcaccac
     2881 gtcgaagatg gtgccctccc gccacttccg gtagtcggcg aacacgccgg gcggcacctc
     2941 gatgtgccgc agctgcacgt acctggtcag cggcaccgcg tccgcggtgg acttggcggc
     3001 ctcgacgaag gtcaacacct ggcggcggaa gtcaccggcg acgtacgggg ccagctcggc
     3061 gaactcgcgc tcgaacagct cggtctcgcc cgccagcgcc tcgaccccgg agagcggggt
     3121 gagccgcagc agcgtcgagc cctccagcga gcggaacagg tggccctcgg ggcggatctc
     3181 ggcccactgc ttcgcggcgg cgtcgagcgc gtcggcggcg gtggtgacct cggtgaccaa
     3241 cagcgtggtc gcggccatgg gactttcctt tcgtcggagg gggttcacca gcggatgagg
     3301 gacatgccca tggccatgcc gccgccgaag gcggtgagca ggaccaggtc gccctcggcg
     3361 atctcgtgct gggcggcggc cagcgtgatg ggcaccgagg cggcgccggt gttgccgtag
     3421 cgctcgaagg tgacgtgggt gcgggcggtc gggaaggcca gggtgcgctc caggtcggcc
     3481 agcatgtggc cgttggcctg gtgcgggacg aagtgcgcga cggtgtcgtc ggcgtacccg
     3541 gtgctggcga ggaacccgcg caccgcgccg gggacctcgc ggtcgacgaa ctcgcgcacg
     3601 gcgcggccgt tcatcgtgaa gtagtgcgcc cccgcggcca gcgactcctt ggtcggcggg
     3661 acgcggcttc cgccaccggc cacctcgatc aggcccgcgt gctgcccgta gctcgacagc
     3721 gacgaggcga cgatgccgcg gccgggccgg gtcgggccga ccaccaccgc gcccgcgccg
     3781 tcgccgaaga gcacggcggt gcgccggtcg gtggggtcga ggatccgcga gtacacgtcc
     3841 accccgacca cgagcgcgtg cccgccgtcg gcgacgagcc tgcgcgcggt ctccagggcg
     3901 tacacgaaac cgctgcacac ggcgttgacg tcgaacgcgg ccgcgttgac cgcgcccagc
     3961 ttgtcctgca ccacgcacgc cgtggccggt tgcggcgagt ccggtgtgga cgtggcgatg
     4021 atgatcaccg acaggtcggt ggcggccagc ccggcccgct cgagcgcctc ctcggcggcg
     4081 ggcaccgcca ggtccgaggc cgcctgccac gacgcggccc ggcgccgggc caggataccg
     4141 gtcttgcgct cgatccacga cgtctccacg ccgacctcgg cggcgacgac gtcgttgccg
     4201 acctcctcct cgggcaggta ggcgccgcag ccgaggatgc cgatgcccga ggtgggcatg
     4261 ggcgtctggt gcacggcggg gccgtcggtc gcctccgcca gcgcgcaccc gcagaagcag
     4321 tcctggctca cgccgccacc cccgcgagcc gcaaccaccg gttgctctcg gcgtgcctgc
     4381 ccagttccgg gtcggcaccc accacgaccg ggtggccgac ctcgcgcagc agcggcaggt
     4441 cgctgacgtg gtcgccgtag ccgaagctca gcggcagcga gaggtcgaac cgctcggcca
     4501 cctcgcgcat cgcgaccgcc ttgaccgcgc cgatcaccga ccggtcgacc gcaccggtga
     4561 gcaggccgtc cgcgccgacc tcgggccggg tgcacagcag gtggtgcgcg ccgagcgcgt
     4621 cggcgagcgg ccgcaggcag gccgggaacg acccgctcac cagcaccacc cggtggcccg
     4681 cgagccggtg ccggaccagc gcgccgacca cccgggggtt gatgaacccc ggtgtgcggc
     4741 ggcgctgctc gaaccactcc cggccctgca ggtccaccag gtcggcgggc acgccgcggt
     4801 agatccggta gtactcgcgg ttggcctcgg cgcggggcag cccgcccccg gccgcggcct
     4861 gctcgaacgc ggcgaaccgc tcggcctgac cgagccgggg gctgcggtcg tcggcgaggt
     4921 ggaatcgcag gaagtcgaac atgctcttgc cggtgatcag cgtttcgtct atgtcgaaga
     4981 aggcggcgac tctcatcggt tcagcactcc ttcgcggtgg tgcgaccgac cagtgattcg
     5041 accgcgctcg tcgcgcggcg cagttccgag cgcttgatcc ggcgatcttc gaaagcggcg
     5101 aaacggatat cggcgtgcgc ggccaccgcg ccggactggg ccagttccat ccgcacccgg
     5161 aatgtcatcc gctccgggtc ctgcacagcg gactcgacga tctcgcaggt gagccgcgcg
     5221 ggcagcggga acaggaacga ctcgaaggtc aggtcgatcg agttccacac cacgtagtag
     5281 tgccgctcgg ggtggcgcac gccgtgctcg gcctcgaaca cggcgatgaa catctgccgc
     5341 atcgcctcgg tgacgaccat gccctgcacg tgctggccgg tctggtggtc gagcagcagc
     5401 tcgttctccc cgtgcaccac caacccggcc acccagtgcc cggccgcgac cgggcgcagg
     5461 tccgagagca gcacgttctg ctcgcggtgc ttgtgcacca gcgacctggc caccggcgcg
     5521 ccgtcgatgc cgagcaccac ggtctcgttg gcgttgcccc gcttggcgag cacgctgtgc
     5581 aggtagtcga gctcgtactc gccgacgccc tggccgacca cgatcgcctc cggcaccggc
     5641 tcgccgcggc gcaggtcggc gacgagctgg ctcagggtgt gcacccccgg caccgcccgc
     5701 atcccggcga accggtcccc caccaggcag accaccgtgg cgccgacctg gtcctcacgg
     5761 ctggctaagt cgatcccgct gctcgcgctg gacatgacaa ataccccctc aggtatcacg
     5821 tgcgttttcg tcgatgattt cgatacccgc gccggaacag atccgtccga cgctggaaca
     5881 attccccgtg ggacgcgcca acgcatcccc tacgagttcg aacgtaacac gtacttcgtg
     5941 acacgagttg tgaagtgcgt caggatgctg ccagttgccg cctcggctca gccttcgttg
     6001 tgccgggctt tccgggagac cagcaggaca atcagcgcgg cggcgacgag aaccgcgccg
     6061 accgccgtga cggaaccgac tccggcggac tccacggcga taccgccgcc gagcgcgccg
     6121 agggcgatcg aggcgttgaa caccgcgacg tagaccgagg acgccgcgtc ggtggcctcc
     6181 ggcgccgacc gcagcaccca ggtctgcagg cacaccggca gcgccgcgta cgacaggccc
     6241 caggtcacca gcagcacgac cgccgcgatg gtcgaggagc caccggtcag cgagatcacc
     6301 agcagcgacg cggccagcag ggccaccgag ctcaccaggg cccggcgcag ccccttcgcg
     6361 atggcgccac cgccgaggaa gttgcccgcg atgcccgccg cgccgaacac cagcagcagc
     6421 acgctgacca tgccgggcga gatgcccacc acgtcctcga ggtagggcgt gacgtaggtg
     6481 taggcgaggt agtggccgat catggtaagt gccgccaccg cgatggccac caggaacggg
     6541 ccgttgcgca gcagttgcgg gatcgcggcc agaccgccgc tgttgcgggc ctgcatggac
     6601 ggcagcagga ccagcaccgc caccaggacg agcaacccgg ccccggcggc cgccaggaac
     6661 gcgacccgcc agttcgtcgc ctgaccgatg gccgtgccca acggcacccc gaccaccgag
     6721 gcgaccgaga tcccgcccag caccacggac agggcgcgca ccgcgttcct cggctcgacc
     6781 aggcgggtgc cgaccgccgg tgtggtggac cagaacacgc cgtgcgcgaa ggcgatcacg
     6841 acccggccga gcatcagcac caggtagttc ggcgcgaccg cggagatgac gttgcccagg
     6901 accagcagcc ccagcagcga caccagcagc gtgcgccggt ccatccgccg cacccacgag
     6961 gtcagcggcg ccgcggtcag cgccgcgata ccgccgtaga cggacacgac gagcccggcg
     7021 atgccgacgg gcacacccac gcccgcgccg atggacggca gcaggccgac ggggagcagt
     7081 tcggtggtga cgaacaggaa cgtgccgagg cccaggaccc agaccgcggg ccaggagcgg
     7141 gggtcggtgt cctcgaccgc ggcgggcggg cgggtcgtgt cgggttgggc ggtcatggtg
     7201 ttgcctcctg ggagtcggcc gccgcgaaca gctccttcgc ctggtacagc gcgttgagcg
     7261 agcgggggat cccgacgtac ggggtgaggt ggacgaacag cgcgacgatc tccgcgcggg
     7321 tggcgcccgc ccgcagcgtg gcggggatgt gcgtgtccag ctgcggcccg cagccgccct
     7381 gcgcggccag cagcgccagg gtgacgagct ggcgggtgcg caggtcgagg ccgggcctgc
     7441 tgacgatgtc gccgaaggcg tactcgacga tcgtggtgcc gaagcggtcg tcgagcgggg
     7501 ccaccgacgc gatcaccgcg tccgggtcga tgcccaggcc cagcatgacc tcccggccga
     7561 cctgctcgcg ctcgctcatg cccgggcctg ctcggtgctt gccgggccgg tgaccggcaa
     7621 ggtggcggcc gggtagcgct gcccgtgcac cttgtcgggg gcgaaggcgg cgtcgagcgc
     7681 ggccacctcg gcgtctgtga gggtgatcgc ggcggcggcc aggttctccc gcaggtggtc
     7741 gaggcggcgg gtgcccggga tcgggatgac gtccacgccc tgcgccagca cccaggcgag
     7801 ggcgatctgc gcgtggctcg cgccgtggcg ctcgacgacc tggtcgatcg cgcccagcaa
     7861 cggcaggttc gcggccaggt tggtctcggt gaagcgcggg ttggtgaagc ggaagtcgtc
     7921 ctcggcgagg ccgtcgcggt cgcgcagtgc gccggtgagg aaaccgcggc ccagcgggga
     7981 gtacgcgacc agcgcggtgc ccagctcacg ggcggcggcg aggacaccgt cggtcaccac
     8041 gtcccgggtc cacagcgaca actcgctctg caccgcggcg atggggtgca cggcgtgcgc
     8101 gcggcgcagc gtggcggcgt cgacctcgga caagccgatg tggcgcacct tgcccgcctg
     8161 gacgagctcg gcgagcgcgc ccacggtgtc ctccaccggc gtggtgccgt cgaggcggtg
     8221 cgcgtagtac aggtcgatgg tgtcgacgcc gagcctgccc aggctggcgt cgcaggcggt
     8281 gcgcaggtag ccggggctgg agtcgttcca ccggcgctca ccgtcgcggc ggatgccgaa
     8341 cttggtggcc acgaacgcgc tgcgggtgcg caggaccgcg ccgacgagcc gctcgttgtg
     8401 cccgccgccg tacatgtcgg cggtgtcgag cagggtcacg cccgcgtcga tggcggcgtg
     8461 cagcacctcg gtcgaccgcg cgtcgtcgtg cgcgccgtag aactcgctca tgcccatgca
     8521 accgaggccg aacaggccga ccgggtcccc tccgaggatc ctggtccgcc cggcctctgg
     8581 gggttgtgct gtctcgctca tgccgccaac cctgctggcc cgcgcggcgc ggcggcagga
     8641 cgcgatccac cctgggagtc ggctacccgg gtgcgccgtc actaggagtg cgctgcctgg
     8701 gtgccgcaca cccaggcagc ggccgggcgc ccgtggcagc atgggtccat ggcgcagcag
     8761 acggacctca gcaccttcct gcggtcgcgc cgggcgaagg tccggcccgc cgacatcggg
     8821 ttgaccaacg ggcacaccgt ccgccgcacg cagggcctgc ggcgcgagga ggtcgccgcg
     8881 tccgccgggg tcagcgtgga ctactacacg cggctggagc aggggcgcga gcgcaacccg
     8941 agcatcgcgg tgctggagtc gctggccacg accctgctgc tggagggcga ggaacgcgac
     9001 cacctgttcc ggctggccgc gcacacgggc aaccgcgccc aggccacgcc gcccgccccg
     9061 cgcgccgtgc ggcccgccgt ccgcaggctg ctcgacaccg tgaccccgtc cccggcgtac
     9121 gtgctgaacc ggtggaacga catgctcgcc gccaacccgg ccgggctggc gctgctggcg
     9181 ggcctggacg agtggccgcc cgcgcgccgc aacacggtcc gctacatctt cctgcacccg
     9241 acggcgccga ccctgttcga ggactggggc tcgatcgccc acaacgccgt ggcgcacctg
     9301 cacgcgatgg agggcctgct gccgggcgcc cccgcgctgc agaccctcgt cgacgagctc
     9361 agcgagcaga gccaggagtt ccgcgccctc tggcagcagc acgacgtccg cagcctgtcc
     9421 accggccgca aactgctcaa ccaccccaac gtcggccgga tggccctgac ctacgaggtc
     9481 ctcgacatca ccaacgacca ccagcgcctg gtcatctacc aggcccccgt aggcacggtc
     9541 gaccacgact cgatgatgct gctcaacctg gtgaacccca gcgcggagac caccgcggag
     9601 cgccccaggc gccggtcgga ccagcgctga tcgtccactg tggagcatcg ccgcgggtgg
     9661 tccgccgcgg tgcaccaggt cgccagcacc gttggcagtc ttggctcctg gctcgaccac
     9721 tacacctgct ccacctatag agcttctccg cagaggccgg tgcaggcgcg gacaaccgcg
     9781 acggtgccga ttcctctgtc tcgcggtggt gctggtcgcg gggagtccgg gctgggtcga
     9841 ggtatcgcga cggtgatgga ggccatagtg gacactcacc tagacctcct gacaaccgca
     9901 ctggggtacc cccgggcgca ctggggagga gtacgtgcgg gcaggcgtcg gggttgggtc
     9961 aggttcctgc tcggccggtg tcgtacgccc acatcgcgat ctcgacccgg ttcctggcgc
    10021 cgatcttggt cagcaggctg gcgacgtggg tcttggcggt ggtcaggccg atgaacagct
    10081 ccgcggcgat ctcggcgttg gtgcggccac cggcgaccag ggccagcacc tgctcctcgc
    10141 gttcggtgag cggctcgatc ggttgggtcc gtcgggtcga cgggtccctg gcggcgaggg
    10201 tggcgagcag tcggcgggtg atgttcggtg cgatgagggc gtcgcccgcg gcggcggcgt
    10261 ggacggcctg gacgagcagg tcctgaccgg cgtccttgag gaggaagccc cgggcgcccg
    10321 cgcgcagcgc gccgtggacg tactcgtcga ggtcgaacgt cgtgatcacc accaccgcga
    10381 tcgggtcggg gacgccccga ccggcgagga gcctggtcac ctcgatgccg tcgagcccgg
    10441 gcatctggat gtcgaccagg cacacgtcgg ggcggagccg gtgcgccagc tcgacggcgg
    10501 cgtggccgtc ggcggcctgg ccgacgacct cgatgtcggg ccgcgcgtcg aggatcatcg
    10561 acaggccggt gcgcaccagg tcctggtcgt cggccaccag cacgcggacc gtcatcggcg
    10621 caccttcacg ggcagttcgg cgtcgaccgc ccacccgccc ccgggagcgg gaccggcccg
    10681 cagcgtgccg ccgagcatct gcacgcgctc ggtcatcccc agcagcccga agccgtggtt
    10741 caccgaccgc cctggggtga tctgtccgtc gtcggtcacg cgcagtcgca gcctt
//
