LOCUS       NZ_CP032698            10405 bp    DNA     linear   CON 04-MAY-2024
DEFINITION  Streptomyces hundungensis strain BH38 chromosome, complete genome.
ACCESSION   NZ_CP032698
VERSION     NZ_CP032698.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces hundungensis
  ORGANISM  Streptomyces hundungensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8393044)
  AUTHORS   Kang,H.J. and Kim,S.B.
  TITLE     Relationship between Morphology and Antimicrobial Activity in
            Streptomyces
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8393044)
  AUTHORS   Kang,H.J. and Kim,S.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2018) Microbiology and Molecular biology, Chungnam
            National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea,
            Republic of
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: RS HGAP Assembly v. v3.0
            Sequencing Technology             :: PacBio RSII
            Assembly Date                     :: 2018-07-09
            Expected Final Version            :: Yes
            Genome Coverage                   :: 130.0x
            Genome Representation             :: Full
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003627815.1-RS_2024_05_04
            Annotation Date                   :: 05/04/2024 02:42:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,472
            CDSs (total)                      :: 7,378
            Genes (coding)                    :: 7,187
            CDSs (with protein)               :: 7,187
            Genes (RNA)                       :: 94
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 191
            CDSs (without protein)            :: 191
            Pseudo Genes (ambiguous residues) :: 0 of 191
            Pseudo Genes (frameshifted)       :: 103 of 191
            Pseudo Genes (incomplete)         :: 121 of 191
            Pseudo Genes (internal stop)      :: 14 of 191
            Pseudo Genes (multiple problems)  :: 42 of 191
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:58:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6542847
            Orig. end                         :: 6553252
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032698.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="28"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5405)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            291..1250
                     /locus_tag="DWB77_RS28975"
                     /old_locus_tag="DWB77_05849"
     CDS             291..1250
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1287: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436204.1"
                     /locus_tag="DWB77_RS28975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_05849"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_120724563.1"
                     /transl_table=11
                     /translation="MPLHRVAVIAPSPVSLFNLAIPELLFGKVEVEGRPAYEVVVCTPE
                     PGPVATTGGLDLYVGRGLDAVEDADTVLVAGTGRRYEPDPRTVAAVGAAAARGVRIASI
                     CSGAFVLAEAGLLDGRSATTYWELAEELRRRYPALDLQGDVLYVQDGNVMTSSGYAAGI
                     DLCLHIIRGDYGAAAANAVARQALVAPVRPGGQTQFTQTPLPAERGVVCADTRGWAMRN
                     LDKPLTLTDLARHAGVSVRTLTRRFHAESGVSPLQWLLHQRVERAKELLETTTLPMDRI
                     AEASGLGSADSLRAHLVRRTGLTPSAYRAAFTRLTTAA"
     gene            1344..2054
                     /locus_tag="DWB77_RS28980"
                     /old_locus_tag="DWB77_05850"
     CDS             1344..2054
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019765416.1"
                     /locus_tag="DWB77_RS28980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_05850"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_120724564.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYIGKARFEKGLADFAHRDDVEVVHRSFELDP
                     NRPKGDVGPVIPMLAEKYGRTLEEARQMEANVASNAHAEGLGYLADGRDHGNTFDIHRL
                     LHLAKERGRQNELLDLAYRANFAEERSVFDAEVLVEIAVAAGLDEAEARTVLADQDAYA
                     DAVRADEREAAELGANAVPFFVLDRTYGISGGQPAEVFTRALEQAWQAHTPALKTIEGD
                     AEACGPEGCEVPRR"
     gene            2208..3257
                     /locus_tag="DWB77_RS28985"
                     /old_locus_tag="DWB77_05851"
     CDS             2208..3257
                     /NRPS_PKS="Domain: Aminotran_5 (15-342). E-value: 7.7e-27.
                     Score: 86.1. Matches aSDomain:
                     nrpspksdomains_DWB77_RS28985_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436207.1"
                     /locus_tag="DWB77_RS28985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_05851"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_120724566.1"
                     /sec_met_domain="Aminotran_5 (E-value: 2.2e-19, bitscore:
                     68.4, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELDSLARGEFAPQTTYLNTASTGLLPARAVTAMSEALAASAAGR
                     PTDMFADVEASRAAYARIVGVPVRRVAAGASVAVYAGLVAASLPEGAEVLLAEADFSSV
                     VNPFHMRQDLKVRTTPLERLAEAVRPGTALVAVSAVQSADGRIADLGAIRDAARAHGAR
                     TLVDMSQGAGWYPLDAGAYDYTVAVGFKWLTTPRGVAFLTVPDDLGGLNPIFAGWVAGE
                     RPWDSCYGPIEELAHSARRFDESPALFSYTGARHSLALVEELGIERIHAHNTALADRFR
                     AGLAPLGHEPVAAPGSAIVSVPGLGHRQEELSGVGVELSNRAGNMRASFHLYTAAEDVD
                     RLLELLPAR"
     aSDomain        2253..3233
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DWB77_RS28985_Aminotran_5.1"
                     /evalue="7.70E-27"
                     /label="DWB77_RS28985_Aminotran_5.1"
                     /locus_tag="DWB77_RS28985"
                     /protein_end="342"
                     /protein_start="15"
                     /score="86.1"
                     /tool="antismash"
                     /translation="TYLNTASTGLLPARAVTAMSEALAASAAGRPTDMFADVEASRAAY
                     ARIVGVPVRRVAAGASVAVYAGLVAASLPEGAEVLLAEADFSSVVNPFHMRQDLKVRTT
                     PLERLAEAVRPGTALVAVSAVQSADGRIADLGAIRDAARAHGARTLVDMSQGAGWYPLD
                     AGAYDYTVAVGFKWLTTPRGVAFLTVPDDLGGLNPIFAGWVAGERPWDSCYGPIEELAH
                     SARRFDESPALFSYTGARHSLALVEELGIERIHAHNTALADRFRAGLAPLGHEPVAAPG
                     SAIVSVPGLGHRQEELSGVGVELSNRAGNMRASFHLYTAAEDVDRL"
     gene            complement(3316..3984)
                     /locus_tag="DWB77_RS28990"
                     /old_locus_tag="DWB77_05852"
     CDS             complement(3316..3984)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017239393.1"
                     /locus_tag="DWB77_RS28990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_05852"
                     /product="hypothetical protein"
                     /protein_id="WP_120724567.1"
                     /transl_table=11
                     /translation="MSLVDKTPRTPVTAADVEHAVRLSVTALRAGLAADWSVPAGSLEW
                     DCWETVEHLSDDLFAYAVQLGPAQPPLDGEVPYLWEAKRPGGPRNAVHADRAAGPAGLL
                     QTLEASGALLTAMVRTTPPEVRAYHGFGVSDPEGFGAMGIVETLLHTHDVAEGLGLTWA
                     APAGLCDRVLARLFRDVPEDQDRWRVLLWASGRADLPGRPRPDTWRWYGEPVGAGAATP
                     "
     gene            complement(4138..4998)
                     /gene="thpD"
                     /locus_tag="DWB77_RS28995"
                     /old_locus_tag="DWB77_05853"
     CDS             complement(4138..4998)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007498585.1"
                     /locus_tag="DWB77_RS28995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_05853"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_174248640.1"
                     /sec_met_domain="PhyH (E-value: 2.3e-39, bitscore: 134.6,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVQDLYPTRGSAEVITPRQDPVVWGPSEGLESFEQDGFLSIQDL
                     LSDDEVALYRSELERLIADPAIRADERSIIEPQSQSVRSVFEVHRISEVFARLVRDERV
                     VGTARRILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPRMRTVSVSIALTE
                     NHDTNGGLMIMPGSHRDFLGCEGATPKDNYKKSLQMQDAGTPSDEALTKMADRHGIQLF
                     TGRAGSATWFDCNAMHGSGDNITPFPRSNVFIVFNSVENTAVEPFAAPIRRPEFIGARD
                     FTPVR"
     gene            complement(5001..5405)
                     /locus_tag="DWB77_RS29000"
                     /old_locus_tag="DWB77_05854"
     CDS             complement(5001..5405)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123624.1"
                     /locus_tag="DWB77_RS29000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_05854"
                     /product="ectoine synthase"
                     /protein_id="WP_120724569.1"
                     /sec_met_domain="ectoine_synt (E-value: 3.7e-54, bitscore:
                     180.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIENTDRHVKAASGTWESKRIVLAKEKVGFSLHETVLY
                     AGTETSMWYANHIEAVLCTEGEAELTNEETGETHWIEPGTMYLLDGHERHTLRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEPEEG"
     misc_feature    complement(5402..5415)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.85"
                     /tool="antismash"
     gene            complement(5467..6735)
                     /gene="ectB"
                     /locus_tag="DWB77_RS29005"
                     /old_locus_tag="DWB77_05855"
     CDS             complement(5467..6735)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 2.1e-71.
                     Score: 232.6. Matches aSDomain:
                     nrpspksdomains_DWB77_RS29005_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018848583.1"
                     /locus_tag="DWB77_RS29005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_05855"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_120724571.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.1e-76, bitscore:
                     255.4, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFESLESEVRSYCRGWPAVFDRAQGARLTDEDGHSY
                     LDFFAGAGSLNYGHNNPVLKRALLDYIERDGITHGLDMATTAKRAFLETFQNVILRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYLDGRVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARP
                     EWLRALADLCRRRDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGLPMS
                     LCLFKGELDVWEPGEHNGTFRGNNPAFVTATAALDAYWADGQMEKHTLARGEQVEQALL
                     ALCTEHGRLGASYRGRGLVWGLEFADPARASEVCARAFELGLLLETSGPKSEVVKLLPP
                     LTITPDELDEGLRSLARAVAATV"
     aSDomain        complement(5641..6651)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DWB77_RS29005_Aminotran_3.1"
                     /evalue="2.10E-71"
                     /label="DWB77_RS29005_Aminotran_3.1"
                     /locus_tag="DWB77_RS29005"
                     /protein_end="365"
                     /protein_start="28"
                     /score="232.6"
                     /tool="antismash"
                     /translation="FDRAQGARLTDEDGHSYLDFFAGAGSLNYGHNNPVLKRALLDYIE
                     RDGITHGLDMATTAKRAFLETFQNVILRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYLDGRVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARPEWLRALADLCRRRDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIVPDIVTVSKSISGYGLPMSLCLFKGELDVWEPGEHNGTFRGNNPAFV
                     TATAALDAYWADGQMEKHTLARGEQVEQALLALCTEHGRLGASYRGRGLVWGLEFA"
     gene            complement(6732..7253)
                     /gene="ectA"
                     /locus_tag="DWB77_RS29010"
                     /old_locus_tag="DWB77_05856"
     CDS             complement(6732..7253)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140785.1"
                     /locus_tag="DWB77_RS29010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_05856"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_120724573.1"
                     /transl_table=11
                     /translation="MTAAQTAVRIENPRVEDGAAIWRIARDSEVLDLNSSYSYLLWCRD
                     FARTSVVARDDDGAPIAFITGYVRPDRPGTLVVWQVAVDHAHRGQGLAGLLLEALTAKV
                     STSHGVTWLETTVSPDNTPSDRLFASYAKRRGASLDRELLFDAGLFPDDGHQPEVLYRI
                     GPLHPQGESL"
     misc_feature    7281..7292
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.18"
                     /tool="antismash"
     misc_feature    complement(7363..7377)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.75"
                     /tool="antismash"
     misc_feature    complement(7387..7402)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     gene            complement(7589..8686)
                     /locus_tag="DWB77_RS29015"
                     /old_locus_tag="DWB77_05857"
     CDS             complement(7589..8686)
                     /NRPS_PKS="Domain: Aminotran_5 (74-297). E-value: 2.6e-28.
                     Score: 90.9. Matches aSDomain:
                     nrpspksdomains_DWB77_RS29015_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018104676.1"
                     /locus_tag="DWB77_RS29015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_05857"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_120728377.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1e-21, bitscore:
                     76.1, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPLLDLAPLTADHFAAIERRVAALLATGQDVVIMQGEALLPLE
                     GCIRGAAHPGSTALNVVTGPYGQTFGNWLRDCGAHVVDLEVPFHTAVTAEQIDRALTDH
                     PEIDFVSLVHAEAATGNTNPVAEIGEVVRRHGALFMLDAVASVAAEPLRVDDWGVDLCV
                     IGAQKAMGGPAGASAVSVSARAWERIAANPNAPRRSYLSLLDWKERWIDGGRKALLHAP
                     AQLEMLALEACVERIESEGLTAVMARHASAAAATRAGALALGGGLAPYVHEAADAAPVA
                     TTLRTPDGVDAGALVAAALAADPSLPLIAGGGALASTMIRVNHYGVDATRGAVQSSLAA
                     LGAALGERGVAVDLEAARRAVSETW"
     aSDomain        complement(7796..8464)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DWB77_RS29015_Aminotran_5.1"
                     /evalue="2.60E-28"
                     /label="DWB77_RS29015_Aminotran_5.1"
                     /locus_tag="DWB77_RS29015"
                     /protein_end="297"
                     /protein_start="74"
                     /score="90.9"
                     /tool="antismash"
                     /translation="RDCGAHVVDLEVPFHTAVTAEQIDRALTDHPEIDFVSLVHAEAAT
                     GNTNPVAEIGEVVRRHGALFMLDAVASVAAEPLRVDDWGVDLCVIGAQKAMGGPAGASA
                     VSVSARAWERIAANPNAPRRSYLSLLDWKERWIDGGRKALLHAPAQLEMLALEACVERI
                     ESEGLTAVMARHASAAAATRAGALALGGGLAPYVHEAADAAPVATTLRTPDGVDAGALV
                     A"
     gene            8800..9884
                     /locus_tag="DWB77_RS29020"
                     /old_locus_tag="DWB77_05858"
                     /pseudo=""
     CDS             8800..9884
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003989359.1"
                     /locus_tag="DWB77_RS29020"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_05858"
                     /product="amidohydrolase family protein"
                     /pseudo=""
                     /sec_met_domain="Amidohydro_1 (E-value: 5.2e-13, bitscore:
                     47.7, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDRMVLHVKGRVLAGPEDVRDELWAVDGRITYERPPGEALTLTG
                     WALPGLVDAHCHVGLDQHGPVPDEVSEKQALTDREAGTLLIRDAGSPSDTRWIDDREDL
                     PKIIRAGRHIARTRRYIRNYAHEIEPGDLVAYIAREARRGDGWVKLVGDWIDREVGDLT
                     PCWPRGEVEAAIAEAHRLGARVTAHCFAEDSLRDLVEAGIDCIEHATGLTEDTIPLFAE
                     RGVAIVPTLVNIATFPHMADGGEAKFPQWAGHMRQLHARRYDTVRSAYDAGVPVFVGTD
                     AGGSLAHGLVAAEVAELVKAGIPALDALSATTWGPAPGWAAPPWRRAPPPTWCSTTRTP
                     ERTSGSSRRPAVWCCAGGWWA"
     gene            <10090..10326
                     /locus_tag="DWB77_RS29025"
                     /old_locus_tag="DWB77_05859"
                     /pseudo=""
     CDS             <10090..10326
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014152420.1"
                     /locus_tag="DWB77_RS29025"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_05859"
                     /product="alpha/beta hydrolase"
                     /pseudo=""
                     /transl_table=11
                     /translation="LAARPALYDVDRLGANTVPVAAAIYQDDMYLDRDLAIGTAGAIRG
                     LRPWITDAYQHDGLRTSGGAVLDQLISLLHGTP"
ORIGIN
        1 gggtgtaggc gtccaccacc cactggaggc cggacatgcc gccgccgaga tcagtgccga
       61 ccgcgggcag ggcgacgttc accacggagg cgtccagggt gatcagggcg aagccgagca
      121 gggcggccgt cagggtgagg gcgggggagg agacgcgcgg cgcccgcggg gtcaaccggc
      181 gttcggtggg cgagatttgg agggaggcgt tcggtgacat gcccccagcc tggcgggcgc
      241 ccggggcgta cagtagtggc ccgaatgcca tacgtccgga ggttctggcc atgccgctgc
      301 accgggtcgc cgtgatcgcg ccctcgcccg tctccctgtt caacctcgcc atccccgagc
      361 tgctcttcgg caaggtggag gtcgaggggc gaccggcgta cgaggtggtc gtgtgcaccc
      421 ccgagcccgg cccggtcgcc acgaccggag ggctcgacct gtatgtgggc cggggcctgg
      481 acgcggtgga ggacgccgac acggtcctgg tcgcggggac gggccggcgc tacgagcccg
      541 atccgcgcac ggtggccgcg gtcggggcgg ccgccgcgcg gggcgtacgc atcgcctcca
      601 tctgtagcgg tgccttcgtg ctcgccgagg cgggactgct cgacgggcgc agcgccacca
      661 cctactggga gctggccgag gaactgcgca ggcgttaccc cgcgctcgat ctccagggcg
      721 atgtcctgta cgtccaggac ggcaatgtca tgacgtcctc cgggtacgcg gccggcatcg
      781 atctgtgcct gcacatcatc cgtggcgact acggcgccgc cgcggccaac gcggtggccc
      841 gccaggcgct ggtcgcgccg gtccgcccgg gcggacagac ccagttcacc cagaccccgc
      901 tgcccgcgga gcgcggggtg gtctgcgcgg acacccgggg ctgggcgatg cgcaacctcg
      961 acaagccgct caccctcacc gacctggccc ggcacgccgg cgtgagcgta cgcaccctga
     1021 cgcgccgctt ccacgccgag agcggggtca gcccgctgca atggctgctg caccagcgcg
     1081 tggagcgcgc caaggaactc ctggagacga ccacgctccc catggaccgg atcgccgaag
     1141 cgagcggcct gggcagcgcg gactccctcc gcgcccacct cgtacgccgc accggtctca
     1201 ccccgagcgc ctaccgggcc gccttcacca gactcaccac ggcggcctga gcgggccgac
     1261 ccggtgggaa gggggcggga atggggcacg gggcgtgcgc gttgtggggg acgtacagcc
     1321 ttcaacaacg tgagagaagt gacatgcgcg tcgagatctg gagcgacatc gcctgcccgt
     1381 ggtgctacat cggcaaggcc cgcttcgaga agggcctggc cgacttcgcc caccgggacg
     1441 atgtcgaggt ggtccaccgc tccttcgagc tcgacccgaa ccggcccaag ggcgacgtcg
     1501 gcccggtgat cccgatgctc gccgagaagt acggccgcac cctggaagag gctcggcaga
     1561 tggaggcgaa cgtcgcctcc aacgcgcacg ccgaggggct cggctacctc gccgacggcc
     1621 gtgaccacgg caacaccttc gacatccacc ggctgctgca cctggccaag gagcgcgggc
     1681 gccagaacga gctgctcgac ctcgcctacc gggccaactt cgccgaggag cgttccgtct
     1741 tcgacgccga ggtcctggtc gagatcgcgg tcgcggcggg cctggacgag gcggaggcgc
     1801 gtacggtcct cgccgaccag gacgcctatg ccgacgcggt gcgcgccgac gagcgcgagg
     1861 cggccgagct cggcgcgaac gccgtcccgt tcttcgtgct cgaccggacg tacgggatct
     1921 ccggcggcca gcccgccgag gtcttcaccc gcgccctcga acaggcctgg caggcgcaca
     1981 ccccggccct caagacgatc gagggcgacg cggaggcgtg cgggcccgag gggtgcgagg
     2041 ttccgcgccg ctgacggcga tcccgcgcgg gttgaggatc cgctgtcgac gcaccgcggc
     2101 cgagcctccg cggtgcggac acggcggccg atacataagg atcgcttggg tgtatcggtc
     2161 aattttaccc aattgacttg ggaatgccgg ggattcagtg tggagccatg gaactggact
     2221 ccctcgcgcg cggcgagttc gcgccgcaga ccacctacct caacaccgcg agcaccgggc
     2281 tgctccccgc ccgggccgtg accgccatga gtgaggccct ggccgcatcg gccgccggcc
     2341 gccccaccga catgttcgcc gacgtggagg cgtctcgcgc cgcgtacgcc cggatcgtcg
     2401 gcgtccccgt gcgacgcgtg gcggccggcg cctccgtcgc cgtctacgcc gggctcgtcg
     2461 ccgcctcgct gcccgaaggg gccgaagtcc tcctcgcgga ggccgacttc agctccgtgg
     2521 tgaatccgtt ccacatgcgc caggacctga aggtgcgcac caccccgctg gagcgcctgg
     2581 ccgaggcggt gcggccgggt accgcgctgg tcgcggtcag cgcggtccag tccgccgacg
     2641 gccggatcgc cgacctcggc gccatccgtg acgccgcccg cgcccacggc gcccggaccc
     2701 tcgtcgacat gtcccagggc gcgggctggt accccctcga tgcgggggcg tacgactaca
     2761 ccgtggccgt cggcttcaag tggctcacca ccccgcgcgg agtcgccttc ctgaccgtcc
     2821 ccgacgacct cggcggcctg aacccgatct tcgcgggctg ggtcgcgggc gagcggccct
     2881 gggactcctg ctacggcccg atcgaggaac tcgcccactc cgcgcggcgg ttcgacgaga
     2941 gtcccgccct gttctcgtac accggggccc ggcactcgct ggccctcgtc gaggaactcg
     3001 gcatcgaacg catccacgcg cacaacaccg cgctcgccga ccgcttccgt gccgggctcg
     3061 ccccgctcgg gcacgagccg gtggccgcgc ccggctccgc gatcgtctcg gtgcccggcc
     3121 tcggccaccg tcaggaggaa ctgagcggcg tcggcgtcga gttgtccaac cgggccggca
     3181 acatgcgggc gtccttccac ctgtacaccg cggccgagga cgtggaccgg ctcctggagc
     3241 tgctgcccgc ccgctgacgg gggagagcgg ccgggcggtc ggctcccggc ctgcgtaggt
     3301 cgttctcggg gaggctcagg gggtggccgc gcccgcgccg accggctcgc cgtaccagcg
     3361 ccaggtgtcc ggacggggcc ggcccggcag gtccgcccgg cccgatgccc acaacagcac
     3421 ccgccagcgg tcctggtcct cgggtacgtc ccggaagagc cgggccagta cccggtcgca
     3481 gagtccggcc ggagcggccc aggtcaggcc gaggccctcg gcgacgtcat gggtgtgcag
     3541 cagggtctcc acgatgccca tcgcgccgaa cccctcgggg tcggagacgc cgaagccgtg
     3601 gtaggcgcgc acctcgggcg gggtggtgcg gaccatcgcg gtgagcaggg cgccgctcgc
     3661 ctccagcgtc tgcaagaggc cggccggacc ggccgcgcgg tcggcgtgca ccgcgttgcg
     3721 ggggccgccg gggcgcttgg cctcccacag gtagggcacc tcgccgtcca gcgggggctg
     3781 ggcggggccg agttgtacgg cgtaggcgaa gaggtcgtcc gagaggtgtt ccacggtctc
     3841 ccagcagtcc cactccagcg aaccggccgg cacgctccag tcagcggcga gcccggcgcg
     3901 cagcgcggtc acggacagcc gcaccgcgtg ctcgacgtcg gcggcggtga cgggagttcg
     3961 gggagtttta tcgaccaggg acatggaggg accgtaccaa cgatctctgt ggacccacct
     4021 gtgcgcaacg ggcccgggga ccggtccggg tgaaccggac ccgtgggggc ggcgccgata
     4081 cggctcccag gccgggggtc cggggaggtg tccccggacc cccggcctga aggtcgttca
     4141 gcgcaccggc gtgaagtccc tcgccccgat gaactccggg cggcggatcg gcgcggcgaa
     4201 gggctccacc gcggtgttct ccacactgtt gaagacgatg aacacgttgc tgcgcgggaa
     4261 cggggtgatg ttgtccccgg agccgtgcat cgcgttgcag tcgaaccagg tcgccgaacc
     4321 ggcccggccc gtgaacaact ggatgccgtg gcggtctgcc atcttggtga gcgcctcgtc
     4381 ggacggcgtt cccgcgtcct gcatctgaag ggacttcttg tagttgtcct tcggcgtggc
     4441 gccctcgcag ccgaggaagt cccggtgcga gccgggcatg atcatcaggc cgccgttggt
     4501 gtcgtggttc tcggtcagcg cgatcgagac cgacaccgta cgcatccgcg gcagaccgtc
     4561 ctcggcgtgc caggtctcga agtccgagtg ccagtagaag ccggaggcgc cgaagccggg
     4621 cttcacattg atccgggact gatggacgta cacgtccgaa ccgaggatgc ggcgcgccgt
     4681 gcccaccaca cgctcgtccc gcaccaggcg cgcgaacacc tcactgatcc ggtgcacttc
     4741 gaagaccgaa cgcacggact gcgactgcgg ttcgatgatc gaccgctcat cggcacggat
     4801 ggcgggatcg gcgatcaatc gctcaagttc cgagcgatag agcgccactt cgtcatccga
     4861 cagcaggtcc tggatcgaga ggaagccgtc ctgctcgaac gattcgagtc cttcggacgg
     4921 tccccagacg accgggtcct ggcggggcgt gatcacctcg gcggagccac gcgtggggta
     4981 gagatcctgg acggtcatgg tcagccctcc tcgggttcgg tcagcagcgg gtagacgccg
     5041 ttctcgtcgt ggtcctcccg tccggtgacc ggcgggttga agacgcagac gcagcggaag
     5101 tcggtcttgg ggcgcagcgt gtggcgctcg tggccgtcca ggaggtacat ggtgcccggc
     5161 tcgatccagt gggtctcgcc ggtctcctcg ttggtgagct cggcctcgcc ctcggtgcac
     5221 agcacggcct cgatgtggtt ggcgtaccac atggaggtct cggtgcccgc gtacaggacg
     5281 gtctcgtgca gcgagaaacc gaccttctcc ttcgcgagga cgatccgctt gctctcccag
     5341 gtgccggagg cggccttcac atggcggtcg gtgttctcga tgtccttgaa cgagcggaca
     5401 atcacggtga gttgctgcct ttctgtctac gtcggtcgga tctgtggcgc cggtcgggcg
     5461 gctgggtcag acggtcgcgg cgaccgcgcg ggccagcgag cgcagcccct cgtccagctc
     5521 gtccggggtg atggtcagcg gcggaagcag cttgaccacc tcgctcttgg ggccggaggt
     5581 ctccaggagc agacccagct cgaaggcgcg ggcacagacc tcggaggcgc gcgcggggtc
     5641 cgcgaactcc aggccccaca ccaggccgcg gccccggtag ctcgcgccga gccggccgtg
     5701 ctcggtgcag agcgcgagca gcgcctgctc gacctgctcg ccgcgggcca gggtgtgctt
     5761 ctccatctgg ccgtcggccc agtaggcgtc gagtgccgcg gtcgcggtga cgaacgccgg
     5821 gttgttgccg cggaaggtgc cgttgtgctc gccgggctcc cacacgtcga gctcgccctt
     5881 gaacaggcac aacgacatcg gcaggccgta gccgctgatc gacttggaga cggtgacgat
     5941 gtccggcacg atgcccgcct cctcgaagga gaagaaggcg ccggtgcggc cgcagcccat
     6001 ctggatgtcg tcgacgatca gcagcatgtc gcggcggcgg cacaggtcgg cgagcgcgcg
     6061 cagccactcg ggccgcgcca cgttgatgcc gccctcgccc tgcacggtct ccacgatcac
     6121 cgcggccggc ttgttgaggc cggagccctg gtcctccagg agccgctcga accacaggaa
     6181 gtcgggcacc cggccgtcga ggtagttgtc gaacggcatc ggcgtgccgt gcaccagcgg
     6241 gatgccggcg ccggcccgct tgaaggcgtt gccggtgacg gcgagcgagc cgagcgacat
     6301 gccgtggaag gcgttggtga aggagacgat cgactcgcgg cccttcacct tgcgggccag
     6361 cttcagcgcg gactcgacgg cgttggtgcc ggtcgggccg gggaacatca ccttgtacgg
     6421 cagatcgcgc gggcgcagga tcacgttctg gaacgtttcg aggaacgccc gtttcgcggt
     6481 ggtcgccatg tcgaggccgt gggtgatgcc gtcccgctcg atgtagtcga gcaacgcccg
     6541 tttcaggacc gggttgttgt gcccgtagtt gagtgagccg gctccggcga agaaatccag
     6601 atagctgtgg ccgtcctcgt cggtgaggcg ggcaccctgc gcgcggtcga acacggcggg
     6661 ccagccgcgg cagtagctgc gtacctccga ttcgagcgac tcgaagacac tgagggcggg
     6721 cggggtgatg gtcacagcga ttctccctgg gggtggaggg ggccgattcg gtagaggact
     6781 tcgggctggt gcccgtcgtc ggggaagagg cccgcgtcga agaggagctc acggtcgagg
     6841 ctggcgccgc ggcgcttggc gtacgaggcg aagaggcggt cggagggggt gttgtccggc
     6901 gagaccgtcg tctccagcca ggtcaccccg tgcgaggtgg acaccttcgc ggtcagcgct
     6961 tcgagcagca gccccgcgag cccctggccc cggtgggcgt ggtcgacggc gacctgccac
     7021 acgacgaggg tgcccggacg gtccggccgt acgtagccgg tgatgaacgc gatgggcgcg
     7081 ccgtcgtcgt cccgggccac cacggacgtc ctggcgaagt cgcggcacca gagcagatag
     7141 ctgtacgagg agttgaggtc gagcacctcg gagtcacggg cgatacgcca gatcgcggct
     7201 ccgtcctcca ctcgtgggtt ttcgatacgg actgctgttt gcgcggcggt catgccgagg
     7261 gaatttaccg atcggaaatt gaaatcgcat tcccgccagg gggtgtgcag ggaagccgtt
     7321 cgtgttatca cgcgagcatg cgcgcgaacc ggtttgtccg gtaattaccg gacaaaaggg
     7381 cgtggttgtg gagtcgatca cacccttgtg accgccggga aaccgtgctg aaactcaggt
     7441 gtttgacttc ggtaagccgg ggcagaagaa aacgggaagc tgcggtggat aagcagcttc
     7501 ccgttatgtg gaacgttttg tggaattcag ttgtgcttgg gctgtgcttc agttgtgctt
     7561 tacgcgtctc agggtcgcgg aaaaggtgtc accaggtttc ggaaacggcc ctgcgggcag
     7621 cttcgaggtc caccgcgaca ccgcgctcgc cgagcgccgc acccagcgcc gcgagcgagg
     7681 actggacggc gccgcgcgtg gcgtccaccc cgtagtggtt gacccggatc atggtcgagg
     7741 ccagcgcccc gccgccggcg atcaacggca gcgacggatc ggccgcgagc gccgcggcga
     7801 ccagcgcgcc ggcgtccacc ccgtcggggg tacgcagcgt cgtggcgacc ggagccgcgt
     7861 ccgccgcctc gtggacatac ggcgcgagcc cgccgccgag cgcgagcgcg cccgcccggg
     7921 tcgccgccgc ggccgaggcg tgccgggcca tcacggccgt caggccctcg gactcgatgc
     7981 gctccacgca ggcctccagg gcgagcatct ccagctgggc gggcgcgtgc agcagggcct
     8041 tgcggccgcc gtcgatccag cgctccttcc agtcgaggag cgagaggtag gagcggcgcg
     8101 gcgcgttcgg gttggcggcg atgcgctccc aggcgcgggc gctgaccgag accgccgagg
     8161 cgccggccgg gccgcccatc gccttctgcg cgccgatcac acacaggtcc acgccccagt
     8221 cgtccacccg gagcggctcg gcggccaccg acgccaccgc gtccagcatg aacagggcgc
     8281 cgtgccggcg caccacctcg ccgatctccg cgaccgggtt cgtattgccg gtcgccgcct
     8341 ccgcgtgaac gagcgaaacg aaatcgatct ccgggtgatc ggtgagcgca cgatcgatct
     8401 gctccgcggt gaccgccgtg tggaacggca cctccagatc gaccacatgg gccccgcagt
     8461 cgcgcagcca gttgccgaag gtctgcccat aggggccggt cacgacgttg agcgcggtgg
     8521 agccgggatg cgccgcgccc cggatgcagc cctccagcgg gagcagcgcc tcaccctgca
     8581 tgatcaccac gtcctgaccg gtggcgagca gggcggccac ccgtcgctcg atcgccgcga
     8641 agtggtcggc ggtcagcggg gccaggtcca ggagcgggtg tgtcacgggg gtgccttctt
     8701 cggtcggctc gggggtcacg gcgaggctcg ggccggggtt cgagcccggt gttcgatcct
     8761 ctggagggag actacccaga ccctcgtaca gtgctgacca tgagcgatcg catggtgctg
     8821 catgtgaagg ggcgcgtcct ggccgggccc gaggacgtac gggacgaact gtgggccgtg
     8881 gacgggcgga tcacctacga gcggcccccg ggcgaggcac tcaccctgac cggctgggcg
     8941 ctgccgggcc tcgtcgacgc gcactgccac gtgggcctcg accagcacgg tccggtgccc
     9001 gacgaggtga gcgagaagca ggccctgacc gaccgggagg ccggaaccct gctgatccgt
     9061 gacgcgggtt cgccctccga cacccgctgg atcgacgacc gcgaggacct gccgaagatc
     9121 atcagggcgg gccgccacat cgcgcgcacc cggcgctaca tccgcaacta cgcccatgag
     9181 atcgagcccg gcgacctcgt cgcctacatc gcgcgggagg cgcggcgcgg ggacggctgg
     9241 gtcaaactgg tcggcgactg gatcgaccgg gaggtcggcg acctcacccc ctgctggccg
     9301 cgcggcgagg tcgaggccgc catcgccgag gcccaccggc tcggcgcccg ggtcaccgcg
     9361 cactgcttcg ccgaggactc gctcagggac ctggtcgagg cgggcatcga ctgcatcgag
     9421 cacgccaccg gcctgaccga ggacaccatc ccgctcttcg ccgagcgggg cgtcgcgatc
     9481 gtgccgaccc tggtcaacat cgccacgttc ccgcacatgg ccgacggcgg cgaggcgaag
     9541 ttcccccaat gggccggcca catgcggcag ttgcacgcgc gccgttacga cacggtgcgc
     9601 tcggcctacg acgcgggcgt acccgtcttc gtcggcaccg acgcgggagg ctccctcgcc
     9661 cacggcctgg tcgccgccga ggtcgccgaa ctggtcaagg cgggcatccc ggcgctcgac
     9721 gcgctctccg cgacgacgtg ggggcccgct cctggctggg ccgccccgcc ctggaggagg
     9781 gcgcccccgc cgacctggtg ctctacgacc aggacccccg agcggacgtc cgggtcctcg
     9841 cggcgcccag ccgtgtggtg ttgcgcgggc gggtggtggg ctgacctcaa ctggccgcta
     9901 ggctaggtga gttgaggtgg atcggcctcc ggggcagcgg ctttcggagg cgcatcgcgg
     9961 gcgcccggga ccaacgggcc gcacgagcgc gggagttggg ggagcgcgca tagtcgtggg
    10021 ccccggccgg gcggtggggg ccgagtgagg cgcggagcga caggcccgat ccctcaccga
    10081 gcgcgtcgct tggccgcacg tcccgccctc tacgacgtcg accgcctggg cgccaacacc
    10141 gtgcccgtcg ccgccgcgat ctaccaggac gacatgtacc tcgaccggga cctggcgatc
    10201 ggcaccgccg gtgccatccg cggactgcgg ccctggatca ccgacgccta ccagcacgac
    10261 ggtctgcgca cgagcggagg agccgtactg gaccagctga tctcgcttct gcacggaacg
    10321 ccctgaagtt gaccgcccgt tacgtccagt gcggccgggt cgttgggatt ctttacgcgt
    10381 acgccggggg cggggcggtg tgggg
//
