LOCUS       NZ_CP032698            11425 bp    DNA     linear   CON 04-MAY-2024
DEFINITION  Streptomyces hundungensis strain BH38 chromosome, complete genome.
ACCESSION   NZ_CP032698
VERSION     NZ_CP032698.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces hundungensis
  ORGANISM  Streptomyces hundungensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8393044)
  AUTHORS   Kang,H.J. and Kim,S.B.
  TITLE     Relationship between Morphology and Antimicrobial Activity in
            Streptomyces
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8393044)
  AUTHORS   Kang,H.J. and Kim,S.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2018) Microbiology and Molecular biology, Chungnam
            National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea,
            Republic of
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: RS HGAP Assembly v. v3.0
            Sequencing Technology             :: PacBio RSII
            Assembly Date                     :: 2018-07-09
            Expected Final Version            :: Yes
            Genome Coverage                   :: 130.0x
            Genome Representation             :: Full
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003627815.1-RS_2024_05_04
            Annotation Date                   :: 05/04/2024 02:42:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,472
            CDSs (total)                      :: 7,378
            Genes (coding)                    :: 7,187
            CDSs (with protein)               :: 7,187
            Genes (RNA)                       :: 94
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 191
            CDSs (without protein)            :: 191
            Pseudo Genes (ambiguous residues) :: 0 of 191
            Pseudo Genes (frameshifted)       :: 103 of 191
            Pseudo Genes (incomplete)         :: 121 of 191
            Pseudo Genes (internal stop)      :: 14 of 191
            Pseudo Genes (multiple problems)  :: 42 of 191
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:58:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2048248
            Orig. end                         :: 2059673
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032698.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11425
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="14"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11425
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11425
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6425](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6425
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    38..51
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.11"
                     /tool="antismash"
     gene            complement(384..608)
                     /locus_tag="DWB77_RS09125"
                     /old_locus_tag="DWB77_01831"
     CDS             complement(384..608)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010059396.1"
                     /locus_tag="DWB77_RS09125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_01831"
                     /product="hypothetical protein"
                     /protein_id="WP_246033472.1"
                     /transl_table=11
                     /translation="MDEMRPHDDTTYCDAGPGAGPGPATEAPAPGSPNEPFAECVLCRE
                     PTEYPESTKGSTLCPVCEWQEAQRTACSG"
     gene            complement(759..1436)
                     /locus_tag="DWB77_RS38805"
                     /old_locus_tag="DWB77_01832"
     CDS             complement(759..1436)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019073972.1"
                     /locus_tag="DWB77_RS38805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_01832"
                     /product="ABC transporter"
                     /protein_id="WP_246033473.1"
                     /transl_table=11
                     /translation="MSPLVPLTRYQSALLLRSQRWLAPVLLYAAFLAVGVQAGEPVLGA
                     LGFAAAGLLPVAAWLVRICVTQEPEAARTCAAAAAGPARVHLASLLAAVGCAALLGLLG
                     TAYVAAVGDGTSADHKVVVPLGSAISAGLLAAGVCVLTGAAVGALGSRPVLHARGWSLT
                     LTALGALLALVLTPSPAKYAVSALVTGSRTGSVPLAVLPLAAALVCAGATAAGVCALSS
                     RRR"
     gene            complement(1433..2242)
                     /locus_tag="DWB77_RS38810"
                     /old_locus_tag="DWB77_01833"
     CDS             complement(1433..2242)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125242.1"
                     /locus_tag="DWB77_RS38810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_01833"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_246033474.1"
                     /transl_table=11
                     /translation="MGRRYSIGGPWVLRGIDLELPEAALVRVGGPNGAGKSTLLRLITG
                     TDAPTEGRVTGRPRTAYVPERFPAALPFTAAGYLTHLGRVHGLSRRAAEERAGHWLERF
                     GAAAHADTGLAELSKGTSQKVAVAQALLAAPELLVLDEAWTGLDTAARAELDRAVRERV
                     TAGATVVFVDHDPARLAGHEDARYTVAGAGLHPAPLSGDPKPSGALVLLDITGTGPLPP
                     QLPGSPQELSWSDGSRTLSVAAEHSDDLLRALLAARPPWHIRGVRPA"
     gene            complement(2266..2943)
                     /locus_tag="DWB77_RS09135"
                     /old_locus_tag="DWB77_01834"
     CDS             complement(2266..2943)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433207.1"
                     /locus_tag="DWB77_RS09135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_01834"
                     /product="AIM24 family protein"
                     /protein_id="WP_120720771.1"
                     /transl_table=11
                     /translation="MKSDLFAIENMAQPATAAGMTLENAKSIKYAVNGEMLARQGAMIA
                     YRGNLQFERRGQGIGGMLKRAVTGEGLPLMAVTGQGEAWFAHEAQNCFIVDVEQGDALT
                     INGRNVLCFDSTLSYEIKTVKGSGIAGGGLFNSVFTGYGRLGLVCEGNPLVIPVSPQLP
                     VYVDTDAVVGWTANLQTSLHRSQSIGSMLRGGSGEAVQLMLQGEGYVIVRPSEATPQKA
                     QQH"
     gene            2942..3763
                     /locus_tag="DWB77_RS09140"
                     /old_locus_tag="DWB77_01835"
     CDS             2942..3763
                     /EC_number="3.1.1.29"
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015579256.1"
                     /locus_tag="DWB77_RS09140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_01835"
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="WP_246033475.1"
                     /transl_table=11
                     /translation="MRASCPGMIRSAKNHRFGGWQTGGVSTQDTAQDTPSAGPFHDESP
                     RDEAPQYVLPLVVHIEKAEPPARTDALETAARAVLVMLSDERSRGEGENEGEWARAVRD
                     WQDARIRKVVRRARGAEWRKASALPGITVTGKAAEVRVFPPVPLDGWPKELAKLQVSGT
                     DLDDPADPGAPDPLAPVLWLNPELDMSAGKAMAQAGHGAQLAWWELTDAEREAWRSGGF
                     ALSVRVAERAAWRELTLSGLPVVRDAGFTEIAAGSCTVVAEHVALRGRLGG"
     gene            4302..>4895
                     /locus_tag="DWB77_RS09145"
                     /old_locus_tag="DWB77_01836"
                     /pseudo=""
     CDS             4302..>4895
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072530.1"
                     /locus_tag="DWB77_RS09145"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_01836"
                     /product="DUF4142 domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MRRVNGTGLLIAVLVATIAALAFPVWSYADRGGTPQATLDSASTP
                     TPWGPLSATDREFVTKVRLAGLWEGPAGQQAQQRAPSAAIKAAGDHLVMGHAALDRHDR
                     DVAAKLGIELPSQPNPQQQGWLRQLTADSGTAYERDFANLLRAAHGKVFALVAQVRATT
                     RNSLVRELANDANTTVLDHMTMLEATGLVDFDALA"
     gene            5001..6425
                     /locus_tag="DWB77_RS09150"
                     /old_locus_tag="DWB77_01837"
     CDS             5001..6425
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003958301.1"
                     /locus_tag="DWB77_RS09150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_01837"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_120720773.1"
                     /sec_met_domain="DUF692 (E-value: 4.1e-94, bitscore: 313.5,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQLGIGIGWRPEIASAVEGLDGIDWVEAVAENLCPGHLPDSLVR
                     LRERGVTVVPHGVSLGLGGADRPDARRLAELAARAEALGSPLVTEHIAFVRAGGTLTAS
                     PRLEAGHLLPVPRTRDALRVLCENVRIAQDALPVPLALENIAALIDWPYEELTEGQFLT
                     ELVERTGVGLLVDVANLHTNRVNRGEDPVKVLDALPLEAIAYVHVAGGVERDGVWHDTH
                     AHPVPRAVLDVLAELAARMDPPGVLLERDEAFPPAAELAGELDAIRSTLRPARRPAPRP
                     AEPVAEPVAGPVSGAGPEAPAEPAARTSAAIRTDATAPEGSRERLALAQAALLSSLTAG
                     TPAPAGFDGPRLRVQARALAAKRADVVAKVAPELVTLLGDGFREAFLGYAKCRPMSGGY
                     RQDALDFARSLLDAGRPGDADTRRALARWWKERAGARPPGRAARLLNTVGGAALGRRNR
                     STYRVGKGVETNSSLT"
     gene            6519..7307
                     /locus_tag="DWB77_RS09155"
                     /old_locus_tag="DWB77_01838"
     CDS             6519..7307
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643868.1"
                     /locus_tag="DWB77_RS09155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_01838"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_120720774.1"
                     /transl_table=11
                     /translation="MRRLLSGTGLVLVALAATLLALLFPLWSYADRSGTPLADLDAATV
                     PTRWGPLSAADRDFVVRVRLAGLWEGPAGQQAIERGPSAAIEAAGRHLVDGHAFLDARV
                     REVAARLGMELPDQPNDQQRGWLSQLTADTGTAYERDFANLLRAAHGKVFSLVAQVRAT
                     TRNSLVRELADDANTTVLDHMRMLEATGLVDFDALARDGASAATASPTGPPPPTPGATL
                     ASPAPLAPEASDSAEPSFSLPPAASRPKDEASSTPDTHTP"
     misc_feature    7329..7347
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 17.66"
                     /tool="antismash"
     gene            7382..8140
                     /locus_tag="DWB77_RS09160"
                     /old_locus_tag="DWB77_01839"
     CDS             7382..8140
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018535540.1"
                     /locus_tag="DWB77_RS09160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_01839"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_120727564.1"
                     /transl_table=11
                     /translation="MFWVLFLLPSIVAAGLSCGRLCLAAVAAARPGPRPEEERDLSCYE
                     AAFLAGGPYRVADLTLISMHRARRLLLAHTGWATVVDPEGRDDLERSVLAAIGPGGQER
                     IATIRSGAAAADAVRALADRLAGAGLAVPDVVRDGVSSSVRAVRGAALLITALGAAALF
                     MPGEASIGRAEMAAWFSLPLVLTLGCLAIARMEIHPYTRWASPDGQALLGGLDAAGDPL
                     TAVAVRGVRAVDDPQVRSALTSGRFAHRSQ"
     gene            8247..9845
                     /locus_tag="DWB77_RS09165"
                     /old_locus_tag="DWB77_01840"
     CDS             8247..9845
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (100-474). E-value:
                     2.7e-22. Score: 71.5. Matches aSDomain:
                     nrpspksdomains_DWB77_RS09165_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019890184.1"
                     /locus_tag="DWB77_RS09165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_01840"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_120720775.1"
                     /sec_met_domain="PF00561 (E-value: 9.7e-28, bitscore: 96.2,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAVALYGTVGSLVLSALVAAPAGSADPLTVEARGAAAAARHAAA
                     TPIAFGRCPEVEALPPSVQCGTVKVPLDYAHPTGRQIALTVSRVKAKGKPAQRQGALVF
                     NPGGPGASSMYFPMVADLAAWKRLAGAYDLIGYSPRGVARSAPLSCQNPADFAQAPSDA
                     PTHPDEPYKMERIAQAQAYAAGCERNAGPALRFYTTLNNTRDLDVLRAALGEKKLTFMG
                     SSYGTYFGAVYATLFPSHVRRMVFDSAVDPDPDQIWYRNNLDQSRAFESRWADFRTWVA
                     KHDDVYHLGATAQEVLESYEKVRAALTRRPAGGTVGPAQLQAAFLKAGYYDDYWSSRAS
                     ALAEYLKGNERPLVQQAAPRPESASDDENGNAVYTAVECNDAPWPTDFATWDRDNSELA
                     RTAPFETWDNVWMNLPCAYWPAPRQKPVDVRVQPGALPPTLILAAERDAATPYPGALEL
                     QRRLPGSVLITEKDAGTHGIAGGANACVNGYVEDYLLNGRTPVRRAECAPHREPNPVSL
                     DERTTHRPGRAVPAL"
     aSDomain        8547..9668
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DWB77_RS09165_Abhydrolase_1.1"
                     /evalue="2.70E-22"
                     /label="DWB77_RS09165_Abhydrolase_1.1"
                     /locus_tag="DWB77_RS09165"
                     /protein_end="474"
                     /protein_start="100"
                     /score="71.5"
                     /tool="antismash"
                     /translation="ALVFNPGGPGASSMYFPMVADLAAWKRLAGAYDLIGYSPRGVARS
                     APLSCQNPADFAQAPSDAPTHPDEPYKMERIAQAQAYAAGCERNAGPALRFYTTLNNTR
                     DLDVLRAALGEKKLTFMGSSYGTYFGAVYATLFPSHVRRMVFDSAVDPDPDQIWYRNNL
                     DQSRAFESRWADFRTWVAKHDDVYHLGATAQEVLESYEKVRAALTRRPAGGTVGPAQLQ
                     AAFLKAGYYDDYWSSRASALAEYLKGNERPLVQQAAPRPESASDDENGNAVYTAVECND
                     APWPTDFATWDRDNSELARTAPFETWDNVWMNLPCAYWPAPRQKPVDVRVQPGALPPTL
                     ILAAERDAATPYPGALELQRRLPGSVLITEKDAG"
     gene            complement(9924..10640)
                     /gene="hemQ"
                     /locus_tag="DWB77_RS09170"
                     /old_locus_tag="DWB77_01841"
     CDS             complement(9924..10640)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951588.1"
                     /locus_tag="DWB77_RS09170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_01841"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_120720776.1"
                     /transl_table=11
                     /translation="MSAAPEKIPNAGKKAKDLNEVIRYTLWSVFKLRDVLPEDRTGYAD
                     EVQELFDQLAAKDITVRGTYDLSGLRADADIMIWWHAETSDELQSAYNLFRRTKLGRAL
                     EPVWSNMALHRPAEFNKAHIPAFLADENPRDYVSVYPFVRSYDWYLLPDEDRRRMLADH
                     GKMARGYPDVRANTVASFSLGDYEWLLAFEADELHRIVDLMRHLRASEARMHVREEVPF
                     YTGRRKSVADLVAGLA"
     misc_feature    10657..10670
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.58"
                     /tool="antismash"
     misc_feature    complement(11137..11155)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 21.57"
                     /tool="antismash"
ORIGIN
        1 gcacaaggac gcggtactgg ccgatgtgga gaaggcgtcg gcccagttca agaaggccgg
       61 agtgaccaac gccgtgccgt acttccgctt ccccggcggc tgctacgacg acgaggtcct
      121 gcgcaccctc gcgccgctga agatgaccgc cgtccagtgg gacgtggtga gcggggacgc
      181 cttcgcgacg gacgccaacg cggtggccga gcaggtgctc gccggggtca ggccggggtc
      241 gctggtggtg atgcactgca cgcgcagcgc ggcgccggtc accgacaccg cgctccagcg
      301 catcgtgccg gagctgcgca agcgcggcta ccgcttcgtg aaggtctccg acctgatccg
      361 cggggccgcg cagcactgag cggtcagccg gagcaggcgg tgcgctgcgc ttcctgccac
      421 tcgcagaccg ggcagagcgt gctgcccttc gtcgactccg ggtactcggt gggctcacgg
      481 cacagcacgc actcggcgaa gggttcgttc ggggagccgg gcgcgggggc ttcggtggcg
      541 ggccccggtc ccgctcccgg tcccgcgtcg cagtacgtcg tgtcgtcgtg cggccgcatc
      601 tcgtccatgg ggccgagcgt aatcgggtac gggtgcacgc ggtggcccgg gcccggcggt
      661 cacccggccc cggcgggggt accgggccga cggtggttcg ggcccggcgg aggtaccggg
      721 ccgacggtgg ttcgggcccg gcgggagtag gcgccggctc agcggcgccg tgagctgagg
      781 gcgcagacac cggcggcggt cgcccccgcg cacaccagcg cggcggccag cggcagcacc
      841 gcgagcggta cggaaccggt gcgcgagccg gtgaccaggg cggacactgc gtacttggcg
      901 ggcgacggcg tgaggaccag ggcgagcagc gcgcccagtg cggtgagcgt gagggaccag
      961 ccccgcgcgt gcagcaccgg ccggctgccc agcgcgccga ccgccgcccc ggtcagcaca
     1021 cagaccccgg ccgccagcag ccccgccgag atcgcggagc ccagcggcac caccaccttg
     1081 tggtccgcgc tcgtcccatc gcccactgcg gccacgtacg ccgtgcccag caggccgagc
     1141 agggccgcgc agccgaccgc cgcgagcagc gacgcgagat ggacccgggc cgggcccgcg
     1201 gcggccgccg cgcaggtgcg ggcggcctcc ggttcctggg tcacgcagat ccgtaccagc
     1261 caggccgcca ccggcagcag cccggcggcc gcgaatccca gcgcgcccag gaccggctcc
     1321 cccgcctgga cgccgaccgc caggaaggcg gcgtacagca ggaccggcgc gagccagcgc
     1381 tgggagcgca ggagcagggc gctctggtag cgcgtgaggg ggacgagcgg gctcatgcgg
     1441 ggcgcactcc tcggatgtgc cagggcgggc gggccgcgag cagggcgcgc agcaggtcgt
     1501 cggagtgctc ggccgccacg gacagggtgc gggagccgtc ggaccagctc aactcctgcg
     1561 ggctgcccgg tagttggggc gggaggggcc cggttccggt gatgtcgagg aggaccaggg
     1621 cgccggaggg cttcgggtcg ccggacaggg gtgcggggtg cagccccgcg cccgccaccg
     1681 tgtaccgcgc gtcctcgtgg ccggcgaggc gcgcggggtc gtggtcgacg aagacgaccg
     1741 tggcgcccgc cgtcacgcgc tcgcgcaccg cgcggtccaa ctcggcgcgg gccgcggtgt
     1801 cgaggccggt ccacgcctcg tccaggacca ggagttcggg ggcggcgagc agcgcctggg
     1861 ccacggcgac cttctggctg gtgcccttgg agagctcggc gagtccggtg tccgcgtgcg
     1921 cggccgcgcc gaaccgttcg agccagtgcc cggcccgctc ctcggccgcg cggcgcgaca
     1981 ggccgtggac acggcccagg tgcgtgaggt aaccggccgc cgtgaacggc agggccgccg
     2041 ggaagcgctc ggggacgtag gcggtgcggg gacggccggt gacccggccc tcggtcggcg
     2101 cgtccgtgcc ggtgatcagc ctcaacaggg ttgattttcc ggcgccgttg gggcccccga
     2161 cacgaaccag ggcggcctcg ggcagctcca ggtcgatgcc gcgcagcacc caggggccgc
     2221 cgatggagta ccggcggccc acgccccgca gtgcgcgggt ggtgctcagt gctgctgcgc
     2281 cttctgcggc gtcgcctcgc tcggccggac gatgacatag ccctcgccct ggagcatcag
     2341 ctggacggcc tcgccggaac cgccgcgcag catcgagccg atggactgcg aacggtgaag
     2401 ggaggtctgg aggttcgcgg tccagccgac caccgcgtcc gtgtcgacgt acaccggaag
     2461 ctgcggggag accgggatga ccagcgggtt gccctcgcag accaggccga gccggccgta
     2521 tccggtgaac acgctgttga acaggccgcc gccggcgatg ccggagccct tgaccgtctt
     2581 gatctcgtag ctgagcgtgg agtcgaagca cagcacgttg cggccgttga tggtgagggc
     2641 gtcgccctgc tccacgtcga cgatgaaaca gttctgcgcc tcgtgcgcga accacgcctc
     2701 gccctggccg gtgaccgcca tcaggggcag gccctcgccg gtcaccgcgc gcttcagcat
     2761 gccgccgatg ccctggccgc gccgctcgaa ctggaggttg ccgcggtaag cgatcatggc
     2821 gccctggcgc gccagcatct ccccgttgac cgcgtacttg atggacttgg cgttctccag
     2881 cgtcattccg gcggcggtcg ccggctgggc catgttctcg atggcaaaaa ggtcgctctt
     2941 catgcgcgca tcctgtcccg gaatgattcg ttccgccaag aatcaccggt tcggcggctg
     3001 gcagactggt ggagtgagca cccaggacac cgcccaggac accccttccg ccggcccttt
     3061 ccacgacgag tccccgcgcg acgaggcgcc gcagtacgtg ctgccgctcg tcgtccacat
     3121 cgagaaggcc gagccgccgg cgcgtaccga cgcgctggag acggcggccc gcgcggtgct
     3181 ggtgatgctc tccgacgagc ggtcgcgggg cgagggcgag aacgagggcg agtgggcgcg
     3241 ggccgtgcgc gactggcagg acgcgcggat ccgcaaggtg gtgcggcggg cccgcggcgc
     3301 cgagtggcgc aaggcctcgg cgctgcccgg gatcacggtg accgggaagg cggcggaggt
     3361 acgcgtcttc ccgccggtgc cgctcgacgg ctggcccaag gaactggcca agctccaggt
     3421 gtcgggcacg gacctcgacg acccggcgga cccgggggcc cccgatccgc tcgctcccgt
     3481 gctctggctc aacccggagc tcgacatgtc cgcgggcaag gcgatggcgc aggcggggca
     3541 cggggcgcag ctcgcgtggt gggagcttac ggatgccgag cgcgaggcct ggcgctcggg
     3601 ggggttcgcg ctctcggtgc gggtcgcgga gcgcgccgcg tggcgcgagc tcacgctgag
     3661 tgggttgcct gtggttcggg atgcggggtt tacggagatt gcggctgggt cttgcacggt
     3721 ggtggcggag cacgtcgcgt tgcgggggcg gttggggggg tgagtgcggg gtgacgtttg
     3781 gggggtgcgt gcggggtgac gtttgggggg tgcgtgcggg gtgacgtttg gggggtgcgt
     3841 gcccccccgg ccccctggcc cgtgccgggc ctggcggcac ccctcttcgg cctcgcgccg
     3901 tgcggctttt tgcgcagttc cccgcgcccc taacccgttt tcggctgcgg gccgtggtcg
     3961 cttctcgcgc agttccccgc gcccctaaac ccgctttcgg cctcgggccg tggtcggtac
     4021 tcgcgcagtt cctcgcgccc ctgactcccg ggcggtgtgc ggccctttgc gcagttcccc
     4081 gcgcccccaa cgcccggctc cgtgcggggc ctcgtgtggc cagcggtgtt ggggggtccc
     4141 gggggcgggt gggggcccgt tcgggctcgg gttctggttg ttgaacgggg gcgcgggggc
     4201 gtgcgtatct ggtggtggcg tcgagtagcg ttcggcgttc tttactgacc ggcacgggag
     4261 gcattttgtc gcgtggcata gttcgctcca gtcgtttcgc cctgcgccgc gtgaacggta
     4321 cgggtctgtt gatcgccgtg ctcgtggcca ccatcgccgc gctggccttt cccgtctggt
     4381 cgtacgccga ccgcggcggc accccgcagg cgactctcga ctcggcctcg acaccgaccc
     4441 cttggggccc gctctccgct acggaccggg agttcgtcac caaggtgcgg ctcgccgggc
     4501 tctgggaggg gccggccggg cagcaggcac agcagcgggc ccccagcgcc gcgatcaagg
     4561 cggccgggga ccacctcgtc atgggccacg ccgcgctgga ccggcacgac cgcgacgtgg
     4621 ccgcgaagct cggcatcgag ctgccgagtc agcccaaccc ccaacagcag ggctggctgc
     4681 gccagttgac ggcggacagc ggtaccgcct acgagcggga cttcgccaac ctgctccgcg
     4741 ccgcgcacgg caaggtgttc gcgctggtcg cccaggtgcg cgcgaccacc cgcaactcgc
     4801 tcgtgcggga gctggcgaac gacgccaaca ccaccgtgct cgaccacatg acgatgctgg
     4861 aggccacagg tctggtggac ttcgacgcgc tggccgtccc cagctgagtc gggcgtccgg
     4921 agcggcgcgg caacctcaaa tcaagctctg aagtggctcg gtcggggttc ggggccgccg
     4981 ccgggggcca tgacctgtcc atgaagcaac tgggcatcgg catcggctgg cggcctgaga
     5041 tcgcctcggc ggtggagggg ctcgacggca tcgactgggt cgaggcggtc gcggagaacc
     5101 tctgccccgg gcacctcccc gactcgctgg tacggctgcg cgagcgcggc gtcaccgtgg
     5161 tgccgcacgg ggtctcgctc ggcctcggtg gcgccgaccg cccggacgcg cggcggctcg
     5221 ccgagctcgc ggcccgggcc gaggcgctcg gctccccgct ggtcaccgag cacatcgcgt
     5281 tcgtgcgcgc gggcggcacg ctgaccgcct cgccgcgcct ggaggcgggc cacctgctgc
     5341 cggtcccgcg cacgcgcgac gccctgcggg tgctgtgcga gaacgtccgc atcgcgcagg
     5401 acgcgctgcc cgtgcccctc gccctggaga acatcgcggc cctgatcgac tggccatacg
     5461 aggagttgac ggagggtcag ttcctgaccg agctcgtcga gcggaccggg gtggggctcc
     5521 tcgtcgacgt cgccaacctc cacacgaacc gggtcaaccg gggcgaggac cccgtgaagg
     5581 tgctcgacgc gctgcccctg gaggcgatcg cgtacgtcca tgtcgcgggc ggcgtggagc
     5641 gggacggcgt ctggcacgac acgcacgccc accccgtgcc ccgcgccgtc ctggacgtcc
     5701 tggccgagct cgccgcacgg atggacccgc ccggggtgct cctggagcgg gacgaggcgt
     5761 tcccgccggc ggcggagctc gcgggcgagc tcgacgcgat ccggtccacg ctccggcccg
     5821 cccggcgccc tgccccgcgc ccggccgaac cggtcgccga accggtggcc gggccagtgt
     5881 ccggggccgg accggaggcc cccgccgaac cggcggcccg gaccagcgca gccatccgga
     5941 ccgatgcgac cgcccccgaa gggagccgtg agcggctggc gctggcgcag gccgcgttgc
     6001 tgtcctcgct caccgccggc acgcccgcgc ccgccgggtt cgacgggccg cgcctgcggg
     6061 tgcaggcccg tgcgctcgcg gccaagcggg ccgacgtggt ggccaaggtg gcgccggagc
     6121 tggtgacgct cctgggcgac ggctttcgcg aggcgttcct cgggtacgcc aagtgccgtc
     6181 cgatgtccgg cggttaccgc caggacgccc tcgacttcgc gcgctcgttg ctcgacgcgg
     6241 gccggccggg tgacgccgac acccggcggg cgctggcccg gtggtggaag gagcgggccg
     6301 gtgcgcggcc gccgggccgc gcggcacggc tcctgaacac cgtgggcggg gcggccctgg
     6361 ggcggcgcaa ccggtcaacg tatcgcgtgg gtaagggagt tgagacgaac tcctccctca
     6421 cgtgacgact tccacaccac ccggccaccc atttcaccga accgcctccc ggatcagcgg
     6481 caaccgcact accgtgcggc acgcaccagg aggcatccat gcgtcgcttg ctgtccggta
     6541 ccgggctcgt cctcgtcgcg ctggccgcga ccctgctcgc gctgctcttc ccgctctggt
     6601 cgtacgccga ccggtccggt acgcccctcg ccgacctcga cgccgcgacg gtgccgaccc
     6661 gctggggccc gctgtccgcg gccgaccggg acttcgtggt ccgggtgcgg ctcgccgggc
     6721 tctgggaggg gccggccggg cagcaggcca tcgagcgcgg acccagcgcc gcgatcgagg
     6781 cggccgggcg gcacctggtg gacgggcacg ccttcctcga cgcccgggtg cgtgaggtcg
     6841 cggcccggct cggcatggag ctgcccgacc agcccaacga ccaacagcgg ggctggctga
     6901 gccagttgac ggcggacacc ggtaccgcct acgagcggga cttcgccaac ctgctgcgcg
     6961 ccgcccacgg caaggtgttc tcgctcgtcg cccaggtgcg cgcgaccacc cgcaactcgc
     7021 tcgtgcgtga gctcgccgac gacgccaaca cgaccgtcct ggaccacatg cgcatgctgg
     7081 aggcgaccgg gctcgtcgac ttcgacgcgc tcgcgcggga cggtgcctcg gcggcgaccg
     7141 cgagccccac cgggccgccc ccgcccacgc cgggggcgac gctcgccagc cccgcaccgc
     7201 tcgcccccga ggcctccgac agcgccgagc cctcgttctc gctgccgccc gccgcgtccc
     7261 gccccaagga cgaggcgtcg tccacacccg acacccacac tccgtaacga aggaataact
     7321 ccacgtccag attgtggaca gactgtggcg ggcaaccgtc aacttcgcat acaactcggc
     7381 catgttctgg gtgctcttcc ttctgccgtc gatcgtcgcg gccgggttgt cctgcggtcg
     7441 gctctgcctc gcggccgtcg ccgccgcgcg ccccggaccg cgtccggagg aggagcggga
     7501 cctgtcctgc tacgaggccg cgttcctggc gggcggcccc taccgggtcg ccgacctcac
     7561 cctgatctcc atgcaccggg cccgtcggct gctgctcgcc cacaccggct gggccactgt
     7621 cgtggacccc gagggccgcg acgacctgga gcggtccgtc ctcgccgcga tcggcccggg
     7681 cggccaggag cggatagcga cgatacggtc cggcgcggcc gcggccgacg cggtgcgcgc
     7741 actcgccgac cggctcgcgg gggcgggcct ggccgtcccg gacgtggtgc gcgacggggt
     7801 cagctcctcg gtgcgggcgg tccggggcgc cgccctgctg atcaccgcgc tcggcgccgc
     7861 ggcgctgttc atgcccggtg aggcgtccat cgggcgggcc gagatggccg cctggttctc
     7921 gctgccgctc gtcctcacgc ttggctgcct cgccatcgcc cgcatggaga tccacccgta
     7981 cacccgctgg gcctcgcccg acgggcaggc gctgctcggc gggctcgacg cggcgggtga
     8041 tccgctcacc gcggtcgccg tgcgcggagt gcgggccgtg gacgatcccc aggtgcgcag
     8101 cgccctgacg agcggtcgct tcgcgcatcg gagtcaatga ccgcgtccgg ggcggcccgg
     8161 ctgctttact tcccctcagg ctaagtatcc ccttttgacc tgtatgtact tcggtagtac
     8221 cgcagcacga aagggatgtt ccggcgatga gagctgtagc gctgtacgga accgtgggtt
     8281 ccctcgtcct ttccgccctc gtcgccgctc ccgcgggcag cgccgacccg ctcaccgtcg
     8341 aggcccgggg cgcggccgcg gccgcccggc acgcggccgc caccccgatc gccttcggcc
     8401 gctgccccga ggtggaggcg ctgcctccct ccgtgcagtg cggcaccgtc aaggtcccgc
     8461 tcgactacgc ccacccgacg ggccgtcaga tcgcgctgac cgtcagccgg gtgaaggcca
     8521 agggcaagcc cgcccagcgg cagggcgccc tggtcttcaa cccgggcggg cccggcgcct
     8581 ccagcatgta cttcccgatg gtggccgacc tcgccgcctg gaagcggctc gccggagcgt
     8641 acgacctcat cggctactcc ccgcgcgggg tggcccgttc ggccccgctg tcctgccaga
     8701 acccggcgga cttcgcccag gccccctccg acgccccgac ccaccccgac gagccgtaca
     8761 agatggagcg catcgcgcag gcccaggcgt acgccgcggg atgtgagcgc aacgcgggtc
     8821 cggcgctgcg cttctacacg accctcaaca acacgcgcga cctcgacgtg ctgcgggccg
     8881 cgctcggcga gaagaagctg accttcatgg gttcctcgta cggcacctac ttcggggccg
     8941 tgtacgcgac gctgttcccc tcgcacgtcc gccggatggt cttcgactcg gcggtcgacc
     9001 cggaccccga ccagatctgg taccgcaaca acctcgacca gtcccgcgcg ttcgagagcc
     9061 gctgggccga tttccgcacc tgggtcgcca agcacgacga cgtctaccac ctgggcgcca
     9121 ccgcgcagga ggtcctggag agctacgaga aggtgcgggc cgccctgacc cgcaggccgg
     9181 ccggcggcac ggtcgggccc gcacagttgc aggccgcgtt cctcaaggcc gggtactacg
     9241 acgactactg gtcgagccgg gcgagcgccc tggcggagta cctcaagggc aatgagcggc
     9301 cgctggtcca acaggccgcc cccaggcccg agtcggcgag cgacgacgag aacgggaacg
     9361 cggtctacac ggccgtcgag tgcaacgacg ccccgtggcc gacggacttc gcgacgtggg
     9421 accgcgacaa cagcgaactc gcgcggaccg cgccgttcga gacgtgggac aacgtgtgga
     9481 tgaacctgcc gtgcgcctac tggccggccc cgcggcagaa gccggtggac gtccgcgtac
     9541 agccgggggc gctgccgccc acgctgatcc tggcggcgga gcgggacgcg gcgacaccgt
     9601 acccgggcgc tctggaactc cagcggcggc ttccgggctc cgtgctgatc acggagaagg
     9661 acgcgggtac gcacggcatc gcgggcggcg ccaacgcgtg tgtgaacggc tatgtggagg
     9721 actacttgct caacggccgg acgccggtgc ggcgcgctga gtgcgcgccg caccgggagc
     9781 cgaacccggt gtcgctggac gagcggacga cccacaggcc ggggcgtgcg gtgcccgctc
     9841 tctgatcttc cgggtcaggg gcgaccggac ccgctcgcgt ggcgcgggcc ggtcacctgg
     9901 tcttgcgtgg ccggtggagg ccgtcaggcg agcccggcga cgaggtcggc gacggacttc
     9961 ctgcggccgg tgtagaacgg cacctcttca cgcacgtgca tccgggcctc ggaggcgcgc
    10021 aggtgacgca tcaggtcgac gatgcggtgc agttcgtcgg cctcgaaggc gagcagccac
    10081 tcgtagtcgc cgagcgagaa cgaggcgacc gtgttggccc gcacgtcggg gtagccgcgg
    10141 gccatcttgc cgtggtcggc gagcatgcgg cggcggtcct cgtcgggcag caggtaccag
    10201 tcgtaactgc gcacgaacgg gtacaccgac acgtagtcgc gcgggttctc gtcggcgagg
    10261 aacgccggga tgtgcgcctt gttgaactcg gcgggacggt gcagcgccat gttcgaccac
    10321 accggttcga gcgcgcggcc cagcttggtg cggcggaaca ggttgtaggc gctctggagc
    10381 tcgtcggagg tctccgcgtg ccaccagatc atgatgtcgg cgtcggcgcg cagacccgac
    10441 aggtcatagg tgccgcggac ggtgatgtcc ttggcggcga gctggtcgaa cagctcctgg
    10501 acctcgtcgg cgtaaccggt gcggtcctcg ggcaggacgt cgcgcagctt gaagacagac
    10561 cacagggtgt agcggatgac ctcgttgagg tccttcgcct tcttccccgc gttgggaatc
    10621 ttttccggag cagcactcat gtggctattg tcccgcgtca ctctgggtgt tccgcgccag
    10681 ggtcgacgtg gcgatgatct cgtcggcggc gcgccgggcg ctcgcgacgc aggcggggat
    10741 gcccacgccg tcgtacgcgg cgccgcagac ccgcagtccg ggcagcttgg cgacctcggc
    10801 gcggacgcgg gcgaccttgg cgagatggcc gaccgggtac tgcggaagtc caccgatcca
    10861 gcggttgacg cggctcgcga cgggtttcgc ggacagcccg acggcctcgc cgagatcgcc
    10921 gagcgagacg tccacgaggt cggcgtcgtc ccagtccagg tggcgctcct cgccgtagcg
    10981 gccgatcgag gtccgcagca cgaagaggtc cgagccgcgt tccttgaccc aactccactt
    11041 gtgcgtcgag aaggtggagg ccttgatggt gtgcccgtcc accggcggca ccagaaatcc
    11101 cgagcgcgtc tccaggccgg ccagatcggc ccgccggaag gcgagcgtga ccaggaccat
    11161 cgaggcgtac tccacctcgg acagctcggc ggccgcgacc ggcgactcgg cggcgagcag
    11221 cgcggaggcc gaccaggcgg gggtggagag gatcaccccg tcggcgtcga tcagttcact
    11281 gtcggtacgg atccgccagc cgtccgcggt ccgggtgagg gcgaggaccg gggtctcggt
    11341 gcggatctcg ccgccctggg cccgtacggc gtcggcgacc gcgacgggca gggtgccgat
    11401 gccgccgtcc aggcccgcga agacc
//
