LOCUS       NZ_CP032698            11935 bp    DNA     linear   CON 04-MAY-2024
DEFINITION  Streptomyces hundungensis strain BH38 chromosome, complete genome.
ACCESSION   NZ_CP032698
VERSION     NZ_CP032698.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces hundungensis
  ORGANISM  Streptomyces hundungensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8393044)
  AUTHORS   Kang,H.J. and Kim,S.B.
  TITLE     Relationship between Morphology and Antimicrobial Activity in
            Streptomyces
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8393044)
  AUTHORS   Kang,H.J. and Kim,S.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2018) Microbiology and Molecular biology, Chungnam
            National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea,
            Republic of
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: RS HGAP Assembly v. v3.0
            Sequencing Technology             :: PacBio RSII
            Assembly Date                     :: 2018-07-09
            Expected Final Version            :: Yes
            Genome Coverage                   :: 130.0x
            Genome Representation             :: Full
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003627815.1-RS_2024_05_04
            Annotation Date                   :: 05/04/2024 02:42:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,472
            CDSs (total)                      :: 7,378
            Genes (coding)                    :: 7,187
            CDSs (with protein)               :: 7,187
            Genes (RNA)                       :: 94
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 191
            CDSs (without protein)            :: 191
            Pseudo Genes (ambiguous residues) :: 0 of 191
            Pseudo Genes (frameshifted)       :: 103 of 191
            Pseudo Genes (incomplete)         :: 121 of 191
            Pseudo Genes (internal stop)      :: 14 of 191
            Pseudo Genes (multiple problems)  :: 42 of 191
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:58:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1139371
            Orig. end                         :: 1151306
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032698.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11935
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="8"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11935
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11935
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6935](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6935)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(76..90)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.75"
                     /tool="antismash"
     misc_feature    76..91
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.43"
                     /tool="antismash"
     gene            complement(405..1964)
                     /gene="proP"
                     /locus_tag="DWB77_RS05165"
                     /old_locus_tag="DWB77_01034"
     CDS             complement(405..1964)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="proP"
                     /gene_functions="transport (smcogs) SMCOG1074: major
                     facilitator family transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010277634.1"
                     /locus_tag="DWB77_RS05165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_01034"
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="WP_120720107.1"
                     /transl_table=11
                     /translation="MVRTLLRRRKKSLSADDVKVADKPAVRRAVSAAALGNTMEWFDFG
                     VYAYVAATLGKVFFPSSSPGAQVISTFATFAAAFLVRPLGGLVFGPLGDRVGRQKVLAV
                     TMIMMAISTFAVGFLPGYKSIGLAAPILLLACRLVQGFSTGGEYAGATTYIAEYSPDRM
                     RGFLGSWLDFGTFVGYSLGSGMVTVLTGLLTEDQMVDWGWRIPFYVAGPLGLIGLYMRL
                     KLDETPAFQEAEERGRRESARGGVTPEEQERLEQARMSGKGRLKEVFTHHWQAMLVCIG
                     LVLLYNVTNYMVTSYLPTYMSTTLGQSELTAQLLVLGTMVFVALTITVVGRSSDRYGRR
                     PLFLWAGAAMVVFSVPVFQLIRQGGVVLPAIGCAVLGLFLVCFAGTSAATLPALFPTHL
                     RYGGLSLAFNFSVSLFGGTTPLVASALVDATGNELVPAYYLMAAGVIGVISALFLRETA
                     GKPLRGSSAMVETEEEAREMVAQSRTHAGRRARDIWLQLRQGHLPHLPHRSDGRDRKDR
                     RG"
     misc_feature    1958..1971
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.13"
                     /tool="antismash"
     misc_feature    1958..1972
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.1"
                     /tool="antismash"
     gene            2325..2981
                     /locus_tag="DWB77_RS05170"
                     /old_locus_tag="DWB77_01035"
     CDS             2325..2981
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PCMT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010070986.1"
                     /locus_tag="DWB77_RS05170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_01035"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_120720108.1"
                     /sec_met_domain="PCMT (E-value: 2.9e-06, bitscore: 25.9,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTLTVRFFQALDRFHAARPWDHNAHYHRWIMRQLPRRFASALDV
                     GSGTGDLARLLAGRAASVRGLDADPEIVDRARELTPSASRVTFAVGDAPGAIAPGPYDV
                     ITCVATLHHLPFAETLAAFRHHLSPGGTLVVVGLARAQSPGDHLLGVAALVMNVVMAWL
                     KHGGRRAARPASMTAATRPATMTFAEIVDEARAVLPGARLRRRLFWRYTLVWHNR"
     gene            3152..4222
                     /locus_tag="DWB77_RS05175"
                     /old_locus_tag="DWB77_01036"
     CDS             3152..4222
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381199.1"
                     /locus_tag="DWB77_RS05175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_01036"
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="WP_120727380.1"
                     /transl_table=11
                     /translation="MTETSHTRSSYWTRFRSHLAGTAPRDTAEAEPEEEPGAPTDLPAR
                     SWGAVLRRTGREFLDDDLPDRAAALTYYGVLSLFPALLVLVSMLGVVGRSATQSLLDNL
                     GHLTPGPVHDLLDSAVRQLQDNGGTSGVIAVVSLLASIWSASSYVAAFIRAANAVYDLP
                     EGRPVWKLTPLRLALTVTLMVLLAISAVIVVFTGPLAQRAGDAIGVGGAAVMTWNIAKW
                     PVLLVLVVLMVALLFWRAPNVHGQGLRWLSPGSLLAVLLWLAASGGFALYVANFGSYNK
                     TYGTLAGLIIFLIWLWLSNLAILLGLELDAELARQRAISDGLPEGEEPYVQPRDTRTWP
                     DKLRRKVKRRHGGPGA"
     gene            complement(4286..4963)
                     /locus_tag="DWB77_RS05180"
                     /old_locus_tag="DWB77_01037"
     CDS             complement(4286..4963)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003958122.1"
                     /locus_tag="DWB77_RS05180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_01037"
                     /product="DUF5825 family protein"
                     /protein_id="WP_120720109.1"
                     /transl_table=11
                     /translation="MSTTLTTTTESLTVAAWRDYDPEACALPGMYLGERELTGPMGEES
                     DRLWESGARRVRLPEPIDLGELDAPGAAERAVRGLSLVRDLTARAVLVEWRLRLAPTAA
                     DDAWRTLSHLQPPQRLEGPADATGALRRWREGHYLCKCLWRQGPGFVQIRDRRWGDLRR
                     FTADEPEYRQTIEALAYGAPAASVPASVLADFTGERLVLEVGELAWWLPYRVNRWIQEA
                     MAI"
     gene            complement(5001..6935)
                     /locus_tag="DWB77_RS05185"
                     /old_locus_tag="DWB77_01038"
     CDS             complement(5001..6935)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004993375.1"
                     /locus_tag="DWB77_RS05185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_01038"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_120720110.1"
                     /sec_met_domain="TIGR03975 (E-value: 4.4e-185, bitscore:
                     615.3, seeds: 14, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 1.8e-10, bitscore: 40.2,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVLLVNMPWSPIDLPSLALGILKRSVDERVPGATAEVLHANLEF
                     TDWITERTEFTADDYEYYALSSYFMGCGDWVFSSALYDDPAWRDEEFATAMKGKLRKSR
                     LRMSQELHRQVPEFVAMIAQRIVEYAPDVVGFTSTFQQNTAALAAAQQVKRLAPHIVTV
                     MGGANCDGEQGEAVHRNFPFVDHVIRGEGESAFPQLVTALAEGAGRDVLDKVPGLCHRD
                     ADGASIVNPMATSPLPPATILAPDYSGYFERLASSVARNWVEPKLVVEGARGCWWGEKH
                     HCTFCGLNGSFMQYRSKSPDTFFEEIMELARRHRVLDMYLVDNILDMGYLTTVLPRIME
                     SGYDLRMHIEIKANMRRSQLRTLAEAGLIYVQPGIESLNNRVLDLMDKGVSGCQNVRML
                     RDGAETGLSVAWNYLHGFPGETAEDYDPVIAQIPALEHLNPPVDLSARIAIERFSPYFN
                     RPELGFDGLRPEEHYRFTYDLPESELYDLAYVFEAPQRGITEPTVTALNDALDAWKKHH
                     ADARLTHTDLGDRIVLVSRRRAFDWRAMELTDPFELAVFRLLDQPHTATALTRKAAARA
                     QGAEREESAVQRLLDSWLALGLVFTDGGQYVHIAPSAVNEDLLRLDFMRHAQAALDAEQ
                     PARPEPLSV"
     misc_feature    complement(7086..7106)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.21"
                     /tool="antismash"
     gene            complement(7139..9016)
                     /locus_tag="DWB77_RS05190"
                     /old_locus_tag="DWB77_01040"
     CDS             complement(7139..9016)
                     /NRPS_PKS="Domain: Abhydrolase_1 (351-498). E-value:
                     3.7e-09. Score: 28.5. Matches aSDomain:
                     nrpspksdomains_DWB77_RS05190_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S9"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020129145.1"
                     /locus_tag="DWB77_RS05190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_01040"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_120720111.1"
                     /sec_met_domain="PF00561 (E-value: 1.7e-06, bitscore: 26.6,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_S9 (E-value: 2.8e-35, bitscore:
                     120.5, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRSYQVYRAALPEVCAGEPTKMVFTADADGRCEVFTWDASTRRA
                     RQVTDRPHGTLHGAIDRDARVWWFDEDANGVGRWRYQPFDGGPDAPGLLGVAEGEPRGL
                     AVGAGGRVAMGLGDTRSTTVHVGPRGGAAREVTRVDGHATLSGTTPDGRLLALSGAAGS
                     AWAVTVLADDGAVCAVLPGDGPEGRLWSLGFAPVADRVELLLVRETNDQYLLASWSPDR
                     GVVTHSWCGFDTEITARWHPDGRSLLVRQDRHGRSGLHRVHLTEGTVEPVPTPRGTLLD
                     AAPRTGSDVHFLWTDTATPPRMVSTAGIELPPTHELPERVPGEHRDLWTPGPDGPVHSL
                     ISLPEGRRGAVPAVFLVHGGPADHDRDAYDGVVHSLVASGFAVVRVNYRGSTGYGPRWR
                     RAFGEGVGLTQVADLVAVRADLVDRGVVRADATGLWGTSWGGYLVLLALGVHPGVWQAG
                     VAVKPVADCAAAHRTTTAALRALDERLFGGTPEEVPERYARSSPLRYAAAVEAPLLVAA
                     ATRDAKCPPGQVRDYLEALRHAGVRHESMWLDTGHDGYEADDHVAVLRRAVVFLDRELR
                     RGRPGTTTPRTTRSDRGGETGRVRSSAPAGTGTTHTERSSGHAEGHHPQ"
     aSDomain        complement(7523..7963)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DWB77_RS05190_Abhydrolase_1.1"
                     /evalue="3.70E-09"
                     /label="DWB77_RS05190_Abhydrolase_1.1"
                     /locus_tag="DWB77_RS05190"
                     /protein_end="498"
                     /protein_start="351"
                     /score="28.5"
                     /tool="antismash"
                     /translation="PAVFLVHGGPADHDRDAYDGVVHSLVASGFAVVRVNYRGSTGYGP
                     RWRRAFGEGVGLTQVADLVAVRADLVDRGVVRADATGLWGTSWGGYLVLLALGVHPGVW
                     QAGVAVKPVADCAAAHRTTTAALRALDERLFGGTPEEVPERYA"
     gene            complement(9013..10296)
                     /locus_tag="DWB77_RS05195"
                     /old_locus_tag="DWB77_01041"
     CDS             complement(9013..10296)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088740.1"
                     /locus_tag="DWB77_RS05195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_01041"
                     /product="MFS transporter"
                     /protein_id="WP_120720112.1"
                     /transl_table=11
                     /translation="MAPGTTAADRRAARRPISRDVRLFWCASAGDALGSQASGVVLPLL
                     LLGLGHSAATVGLIAGVSTAAGLLLAPIAAVPADRGARKPVMFWSATVSALAMAGLAAA
                     VTRGNPPLALLLGTVLVERFSTAVYEAAAAGTVAMIAPPADCPRVVAGLSAGDQGALIL
                     GPALGGALYQASRALPFLADAVSYAVAAGCVRALSSDLRPRHGTAPKDGAAPDADPAPR
                     TGPARRMWDELGAGLALVRTSPFLRLALTWTATVNGVIVALYYGAVFTLQKGGHGGAPM
                     GLVLALSGAAGLIGSLAAPRVVGRIGAARLLVTVSWLLVPLAAGLATAEGPWTYGALFG
                     VFCLLMPLVAVVLQSRALQETPPALQARTGAVLASAVGGSAALAPVLAGVFVTRAGTAA
                     PAAVCAALLVALAWRTTVVRGRLTGATA"
     misc_feature    10367..10381
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 18.19"
                     /tool="antismash"
     misc_feature    complement(10489..10500)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.41"
                     /tool="antismash"
     gene            complement(10924..11724)
                     /locus_tag="DWB77_RS05200"
                     /old_locus_tag="DWB77_01042"
     CDS             complement(10924..11724)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020126249.1"
                     /locus_tag="DWB77_RS05200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DWB77_01042"
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="WP_120720113.1"
                     /transl_table=11
                     /translation="MPTPGRTRTRNHDDAPDTVEAFARLTTLEPGPAHDALRDDLVAQW
                     LPMARRLAGRYRNRGAELEDLHQVAAMGLVKAVDRYEAARGAFEAYAIPTIDGELKRHF
                     RDHLWAVHVPRRVQQLRNKVRVARRDLQDVRPGEPTIADLAARCGLDEQDVREGLEALE
                     SYRSLSLDVESRGGDGDGPALADTLGTPERGFDTVVDRESVKPALRALPERECRILHMR
                     FFQDMTQHQIAAELGISQMHVSRLLSSTCTRIRNQALAEYRHAA"
ORIGIN
        1 gtcggtgggg acgccgcggc cctctacctc caattggcga cgctcgccgc acccaccgac
       61 cgcgccgtgc ggcgctggaa cggctggtcg gccaagcgcc acagggaggt gtgcgccgag
      121 gtgctcggca cgggcagggt ggttcaggcc aagcgggcac gggccggccg gagcctgttc
      181 ctgcccggcc catggaccga actcaaggcc ccgcacctgc cgttggagac cgcgaagctt
      241 gccccgcacg cggtgcgccc gctgtggggc aaccagatcc tcgccccgtt cggccgcgtc
      301 ctgccgacgg cgccgcttca cgagatgttc gctgacgcgt gggcgcggct ggcgatggcc
      361 ggcggggtgg gcgctcctga catggcgccg tcgtaggagg ggcgtcagcc gcgccggtcc
      421 ttgcggtcgc ggccgtccga gcggtggggc aggtgcggca ggtggccctg gcgcagctgg
      481 agccagatgt cgcgggcccg gcggcccgca tgggtgcggc tctgggccac catctcgcgg
      541 gcctcctcct cggtctcgac catcgcggac gatccccgca gcggtttgcc cgcggtttcg
      601 cgcaggaaca gggccgagat cacgccgatc acgcccgcgg ccatgaggta gtaggcgggc
      661 acgagttcat tgccggtcgc gtcgacgagg gcggaggcca cgagcggggt ggtgccgccg
      721 aacagggaca cggagaagtt gaacgcgagg gacagcccgc cgtagcgcag atgggtcggg
      781 aagagcgcgg gcagggtggc cgcgctggtc ccggcgaagc acaccaggaa cagcccgagg
      841 acggcgcagc cgatggcggg caggacgacg ccgccctggc ggatgagctg gaagacgggc
      901 accgagaaga cgaccatcgc ggccccggcc cacaggaaga gcgggcggcg gccgtagcgg
      961 tccgacgacc ggccgacgac cgtgatggtg agggcgacga acaccatcgt gccgaggacg
     1021 aggagctgcg cggtgagctc ggattggccc agcgtggtgc tcatgtaggt cggcagatag
     1081 gaggtcacca tgtagttggt gacgttgtag agcagcacca ggccgatgca gacgagcatg
     1141 gcctgccagt ggtgggtgaa gacctccttg agccggccct tgcccgacat gcgtgcctgt
     1201 tccaggcgtt cttgctcctc gggggtgacg ccgccccgcg cggactcccg ccgtcctcgc
     1261 tcctcggcct cctggaacgc gggcgtctcg tccagcttga gccgcatgta gaggccgatg
     1321 agcccgagcg gcccggcgac gtagaacggg atccgccatc cccagtcgac catctggtcc
     1381 tcggtcagga gcccggtcag aacggtcacc atgccggagc cgagcgagta gccgacgaag
     1441 gtgccgaagt cgagccagct gccgaggaag ccccgcatcc ggtcgggcga gtactcggcg
     1501 atgtacgtgg tggcaccggc gtactcaccg ccggtcgaga agccctgcac cagtcggcag
     1561 gccagcagca ggatgggcgc ggcgaggccg atggacttgt agcccggcag gaagcccacc
     1621 gcgaaggtgc tgatcgccat catgatcatg gtgacggcga ggaccttctg gcgtcccacg
     1681 cggtcgccga gcgggccgaa cacgagcccg ccgagcggcc ggacgaggaa ggcggcggcg
     1741 aacgtcgcga acgtcgagat gacctgggcg cccggcgaac tcgacgggaa gaagaccttg
     1801 ccgagggtgg cggcgacata ggcgtagaca ccgaagtcga accactccat ggtgttgccg
     1861 agcgcggcgg ccgacacggc ccggcgcacg gcgggcttgt cggccacctt gacgtcgtcc
     1921 gcgctcaggc tcttcttgcg acggcgcagc agcgtccgga ccatgcgttc ggtctgcggg
     1981 cttcccgagc cgaacccgcg acctcgatca cccgggggtg tgggcccccg cctgcttgat
     2041 ttcatgggca cctgtgcgtc gtcgtcggtg gttgcacacc aggcctggtg cgatgtacgg
     2101 agtgctgccg ggcccccgga ccgcgtgtcc cgtacccggc atacgtacat caactctccc
     2161 ccgcggacac ggtgcgcgca gcgcaagcgg ggcggcgtgg gcgtgtcggc gcggacgcag
     2221 gccgacgcgc cggctcgcgg cgccgccgcc gcgatcgttg gcaggggggt ctgtggtcgc
     2281 gcccccgttc cggcaccccg agccgggaat gatggggttg cgccatgacg actctgacgg
     2341 tccgtttctt ccaggccctc gaccgcttcc acgccgcccg cccgtgggac cacaacgccc
     2401 actaccaccg atggatcatg agacagctcc cccggcgctt cgccagcgcc ctggacgtcg
     2461 gttcgggcac gggcgatctg gcccggctcc tggcgggccg cgccgcctcg gtgcggggtc
     2521 tggacgccga ccccgagatc gtcgacagag cacgtgagct caccccgtcg gcgtcacggg
     2581 tgaccttcgc cgtcggcgac gcgccgggcg cgatcgcacc gggcccctat gacgtcatca
     2641 cctgcgtcgc caccctgcac catctgccgt tcgccgagac cctcgccgcc tttcgccacc
     2701 acctgtcccc cggcggcacg ttggtcgtcg tggggctcgc ccgcgcgcag tcacccggcg
     2761 accatctgct gggcgtcgcc gccctggtga tgaacgtcgt catggcctgg ctcaagcacg
     2821 gcggccgcag ggccgcgcgg ccggcctcca tgaccgctgc cacccgcccg gcgaccatga
     2881 ccttcgccga gatcgtcgac gaggcacgcg cggtgctgcc cggagcccgt ctgcggcggc
     2941 gcctgttctg gcgctacacg ctggtgtggc acaaccgctg acccggagcg ggggcgcggg
     3001 ccggaacctc atccgccccc gagcggcggg cgcaccctga cggcgccggt caacctaccc
     3061 cgcggccgtg acgccacccc catccgagcc gggcgagcgg tggtcgggcc ctggggcgcg
     3121 tgagcggcgt cttccagggg actcggtgtt catgacagag acctcgcaca cccggagttc
     3181 gtactggacc cgattccgct cccacctggc cggcaccgcg ccacgggaca cggcggaggc
     3241 ggagccggag gaggaacccg gcgcccccac cgatcttccg gcccggtcat ggggggcggt
     3301 gctgcgccgt accggccgcg agttcctcga cgacgacctt cccgaccggg cggccgcgct
     3361 cacctactac ggggtgctgt ccctgttccc cgcgctgctg gtgctggtgt cgatgctggg
     3421 cgtcgtcggc aggagcgcga cgcagtcgct cctggacaac ctgggccatc tcacgccggg
     3481 cccggtgcac gatctgctcg acagcgccgt ccgtcagttg caggacaacg gcggcaccag
     3541 cggggtgatc gcggtggtga gcctgctcgc ctcgatctgg tcggcttcga gctatgtcgc
     3601 cgcgttcatc cgcgccgcga acgccgtgta cgacctgccg gagggccgac cggtgtggaa
     3661 actgacgccg ctgcggctgg cgttgacggt cacgctgatg gtgctgctcg cgatcagcgc
     3721 ggtgatcgtg gtctttacgg gacccctggc gcagcgcgcg ggggacgcga tcggagtggg
     3781 cggcgccgcg gtcatgacgt ggaacatcgc gaagtggccg gtgctgctgg tcctggtggt
     3841 cctcatggtg gctttgctgt tctggcgcgc ccccaacgtc catggccagg gcctgcggtg
     3901 gctgagcccg ggcagtctgc tcgcggtcct gctgtggctg gcggcgtcgg gcggcttcgc
     3961 gctgtacgtg gccaacttcg gctcgtacaa caagacgtac ggcacgctgg cgggcctcat
     4021 catcttcctg atctggctgt ggctgtcgaa cctggcgatc ctgctcggtc tggagctgga
     4081 cgcggagctg gcccgtcagc gcgccatcag cgacggactg cccgagggcg aggagcccta
     4141 tgtgcagccc cgcgacaccc ggacgtggcc ggacaaactg cggcgcaagg tgaagcgccg
     4201 ccacggcgga cccggcgcct gagtcgcacc cccgatggtg ccgccccaac ccccttgtgc
     4261 agcaagggaa ttggggcggc cgcactcaga tcgccatggc ctcctggatc cagcggttca
     4321 cccggtacgg cagccaccag gcgagctcgc cgacctccag gaccaggcgt tcccccgtga
     4381 agtcggccag cacggaggcg ggtaccgagg ccgccggggc tccgtaggcg agggcctcga
     4441 tggtttggcg gtactccggc tcgtcggcgg tgaagcggcg caggtcaccc cagcggcggt
     4501 cacggatctg gacgaagccc gggccctgcc gccacaggca cttgcagagg tagtggccct
     4561 cgcgccagcg gcgcagagcg ccggtcgcgt cggcggggcc ctccaggcgt tgcggcggct
     4621 ggaggtggct gagggtgcgc caggcgtcgt cggcggccgt cggggcgagg cgcagtctcc
     4681 actcgacgag gacggcccgc gcggtcaggt cccgcaccag gctcagaccg cgtacggcgc
     4741 gctcggccgc gccgggggcg tcgagttcac cgaggtcgat gggttcgggc agccgtaccc
     4801 ggcgggcgcc gctctcccac agccggtcgc tctcctcgcc catggggccg gtcagttccc
     4861 gctctccgag gtacatcccg ggcagcgcgc acgcctcggg gtcgtagtcg cgccaggcgg
     4921 cgaccgtgag ggactcggtc gtggtggtga gggtggtgct catcggtcca gtcctcctgc
     4981 tcctgtgtcg gacacgcggc tcagacgctc aacggctccg gccgggcggg ctgttcggcg
     5041 tcgagcgcgg cctgcgcgtg acgcatgaag tccaggcgca gcaggtcctc gttgacggcc
     5101 gagggcgcga tgtggacgta ctggccgccg tcggtgaaga ccaggccgag cgcgagccag
     5161 gagtcgagga ggcgctggac cgcgctctcc tcgcgctcgg cgccctgggc gcgggcggcc
     5221 gccttgcggg tgagcgcggt ggccgtgtgg ggctggtcga ggagtcggaa gacggccagt
     5281 tcgaacgggt cggtcagttc catcgcgcgc cagtcgaagg cgcgccgcct gctcaccagg
     5341 acgatgcggt cgccgaggtc ggtgtgggtg agccgggcgt cggcgtggtg tttcttccag
     5401 gcgtcgaggg cgtcgttgag ggcggtgacg gtgggctcgg tgatgccgcg ctggggcgcc
     5461 tcgaagacgt aggcgagatc gtagagctcc gactcgggca ggtcgtaggt gaagcggtag
     5521 tgctcctcgg ggcgcagccc gtcgaagccg agctcgggcc ggttgaagta cgggctgaac
     5581 cgctcgatcg cgatccgcgc ggacaggtcg accggcgggt tgaggtgttc gagtgcgggg
     5641 atctgcgcga tgacggggtc gtagtcctcg gcggtctcgc cggggaagcc gtggaggtag
     5701 ttccaggcga cggagaggcc ggtctcggcg ccgtcgcgga gcatgcggac gttctggcag
     5761 ccgctgacgc ccttgtccat caggtccagg acccggttgt tgaggctctc gatgcccggc
     5821 tgcacgtaga tcagtccggc ctcggcgagc gtccgcagtt ggctgcgacg catgttggcc
     5881 ttgatctcga tgtgcatccg caggtcgtag ccgctctcca tgatgcgggg cagcacggtg
     5941 gtgaggtagc ccatgtcgag gatgttgtcg acgagataca tgtcgaggac cctgtggcgg
     6001 cgggcgagtt ccatgatctc ttcgaagaag gtgtccgggc tcttggagcg gtactgcatg
     6061 aacgagccgt tgaggccgca gaacgtgcag tggtgcttct ccccccacca gcagccgcgc
     6121 gcgccctcca ccaccagctt gggctcgacc cagttgcggg ccacggagga ggcgaggcgc
     6181 tcgaagtagc cgctgtagtc gggggcgagg atcgtggccg gcggcagggg gctcgtggcc
     6241 atcgggttga cgatgctcgc gccgtcggcg tcccggtggc acaggcccgg caccttgtcc
     6301 aggacgtcgc gcccggcccc ttcggcgagg gcggtcacca gctgggggaa ggcggactcg
     6361 ccctcgcccc ggatgacgtg gtcgacgaag gggaagttgc ggtggacggc ctcgccctgc
     6421 tcgccgtcgc agttggcgcc gcccatgacg gtcacgatgt gcggggcgag ccgtttgacc
     6481 tgctgggcgg cggcgagggc ggcggtgttc tgctggaagg tggaggtgaa gccgacgacg
     6541 tcgggcgcgt actccacgat cctctgggcg atcatcgcca cgaactccgg tacctggcgg
     6601 tgcagttcct gcgacatgcg aaggcgtgac ttgcggagtt tgcccttcat cgcagtcgcg
     6661 aactcctcgt cgcgccaggc ggggtcgtcg tagagggcgg aggagaacac ccagtcgccg
     6721 cagcccatga aataggagga gagcgcgtag tactcgtagt cgtcggcggt gaattcggtg
     6781 cgctcggtga tccagtcggt gaattcgaga ttggcgtgca gtacttcggc ggtggcgccg
     6841 ggcacgcgtt cgtccacgct gcgcttgagg attcccaggg ccagcgaggg aaggtctatg
     6901 ggcgaccagg gcatattgac cagcaggaca cgcatgtcgg ctcctcggga tgtgcggaga
     6961 cgtagggagt ggggcccggg gggccggagt tggggggtct tgccggccgg cccggcggcg
     7021 aaccgccggg ccggccggca aggatcagtc ctcttcgccg tcctcggcgt tacggaacgg
     7081 gaccttgatg gtcacgccga cgctcggctt gcggttttcc tcgtcacccg cgagcgggtc
     7141 attgtggatg atgtccttct gcatggccgg aactcctctc tgtgtgggtg gtgcctgttc
     7201 cggcgggcgc cgatgagcgc acccgtccgg tctccccgcc ccggtcggag cgggttgtcc
     7261 tgggggtggt ggtgccgggc cgcccgcgcc gcagttcgcg gtcgaggaag accaccgccc
     7321 ggcgcagcac ggccacgtgg tcgtcggcct cgtagccgtc gtggccggtg tccagccaca
     7381 tcgactcgtg gcgtacgccc gcgtgccgaa gggcttcgag gtagtcgcgc acctggccgg
     7441 gcgggcactt ggcgtcccgg gtcgcggcgg cgacgagcaa gggggcctcg acggcggccg
     7501 cgtaacgcag gggcgaactc cgcgcgtacc gctcgggcac ctcctcgggg gtgccgccga
     7561 acagccgctc gtccagggcg cgcagggccg cggtggtggt gcggtgggcg gccgcgcagt
     7621 ccgcgacggg cttgacggcg acgcccgcct gccacacgcc ggggtggacg ccgagggcga
     7681 gcaggaccag atagccgccc caggaggtgc cccacagtcc ggtcgcgtcc gcgcggacca
     7741 ccccgcggtc gacgaggtcg gcgcggaccg cgacgaggtc ggcgacctgg gtgagcccga
     7801 cgccctcgcc gaacgcccgc cgccagcgcg gcccgtaccc ggtggagccg cggtagttga
     7861 cgcggacgac ggcgaagccg gaggcgacca gcgagtggac caccccgtcg taggcgtccc
     7921 ggtcgtggtc ggcggggccg ccgtgcacca ggaacaccgc gggcaccgcg ccccgccgcc
     7981 cttcgggcag gctgatcagc gagtgcaccg ggccgtcggg tccgggggtc cacaggtccc
     8041 ggtgctcgcc gggcacccgc tcgggcagct cgtgggtggg aggcagctcg atgccggcgg
     8101 tggagaccat gcggggcggt gtcgccgtgt ccgtccacag gaagtgcaca tcggatccgg
     8161 tgcgcggcgc cgcgtcgaga agggttccgc gcggtgtcgg tacgggctcg acggtgccct
     8221 ccgtcaggtg cacgcggtgc aggccgctgc gcccgtgccg gtcctggcgc accaggagcg
     8281 agcggccgtc ggggtgccag cgggcggtga tctcggtgtc gaagccgcac caggagtgcg
     8341 tgaccactcc cctgtcgggg ctccaactgg ccagcagata ttggtcgttg gtctcgcgca
     8401 cgaggaggag ttcgacgcgg tcggcgacgg gggcgaaccc cagcgaccag agccgccctt
     8461 cggggccgtc cccgggtagc accgcgcata cggcgccgtc gtcggcgagg acggtcacgg
     8521 cccacgccga tcccgcggcg ccgctgagcg cgaggaggcg gccgtcgggg gtggtcccgg
     8581 acagggtggc gtgcccgtcg acgcgcgtca cttcgcgggc ggcgccaccg cgcggaccca
     8641 cgtggacggt ggtcgaccgg gtgtcgccga gccccatcgc gacgcggccg ccggcaccga
     8701 cggcgaggcc acgcggctcg ccctcggcca caccgagcag gcccggtgcg tccggtccgc
     8761 cgtcgaaggg ctggtaccgc cagcgcccga cgccgttggc gtcctcgtcg aaccaccaca
     8821 cccgtgcgtc ccggtcgatc gcgccgtgca gggtgccgtg cgggcggtcg gtgacctgac
     8881 gggcccggcg cgtgctggcg tcccaggtga acacttcgca gcggccgtcg gcgtcggcgg
     8941 tgaacaccat cttggtgggt tcaccggcgc acacctcggg cagcgccgcg cggtagacct
     9001 ggtagctcct ggtcatgcgg tcgcccccgt cagccggccg cgtacgacgg tggtccgcca
     9061 ggccagcgcc acgagcaggg cggcgcagac cgcggcgggc gcggccgtcc cggcgcgggt
     9121 cacgaacacc ccggccagga ccggcgccag cgccgccgaa ccgcccaccg cgctcgccag
     9181 caccgcgccc gtgcgggcct ggagggcggg cggggtctcc tggagggcgc gggactggag
     9241 cacgacggcg accagcggca tcagcaggca gaacaccccg aacagcgcgc cgtacgtcca
     9301 agggccctcg gcggtggcga gcccggcggc gagcggaacg agcagccagg acaccgtcac
     9361 cagcagccgc gccgcgccga tccggcccac gacacgcggc gcggccagcg acccgatgag
     9421 acccgcggcg ccggagagcg cgagcaccag gcccatcggt gcgccgccgt ggccgccctt
     9481 ctggagggtg aacaccgcgc cgtaatagag cgcgacgatc accccgttga cggtggcggt
     9541 ccaggtcagg gcgagccgga ggaagggcga ggtgcggacc agggcgagcc cggcccccaa
     9601 ctcgtcccac atccggcgcg cggggccggt ccggggcgcg ggatccgcat cgggggcggc
     9661 gccgtccttc ggggccgtgc cgtgccgcgg cctcaggtcg gagctcaacg cccgtacgca
     9721 tccggcggct acggcgtacg acaccgcgtc cgcgaggaag ggcagggcgc gggacgcctg
     9781 gtagagcgcg ccgccgaggg ccgggcccag gatcagtgcg ccctggtccc cggccgaaag
     9841 cccggccacc acgcgggggc agtcggcggg cggggcgatc atcgccacgg tgccggcggc
     9901 ggcggcctcg tacaccgcgg tggagaaccg ttccaccagg accgtcccga gcagcagcgc
     9961 gagcggcggg ttcccccggg tcacggcggc cgcaaggccg gccatggcga gtgccgagac
    10021 ggtcgcggac cagaacatca cgggtttgcg cgcgccccgg tcggcgggga cggccgcgat
    10081 cggggcgagc agcagcccgg ccgccgtgga cacccccgcg atcaggccga cggtcgccgc
    10141 cgagtggccg agcccgagga gcagcagggg caggacgacg ccggacgcct gggagccgag
    10201 ggcgtcaccg gcgctcgcgc accagaacag ccggacgtcc cgggagatcg gccgtcgggc
    10261 cgcccgtcgg tcggcggccg tggtgccggg ggccatcaga gcgccgcccc tacagaggtc
    10321 cctggaaggc ccagggactc cggttcacgc acagccaccc cacagttggt caagttgcga
    10381 agaatatgtg caggaatcga aacgagcggg agcctaacca ggcgcccgct cggctcacaa
    10441 gggttccgcc atcgaggccg gaatcgaact ccgtaagggc caggcaaaga aaccgattgc
    10501 ccgctccccg ggacggcccc gatccgcccc cggccccgcc cgggacgaaa gggaaaagga
    10561 gcgggaccac cgccgaccgc cggatacccc cgccgctccc ccgcctccgc ttcgcagcgg
    10621 cgaacatcgg tcccgcgcgc ggcagttcgc gcagcacgtc cggcatatgc gccttcccgg
    10681 cggcgaacct gccggtgttc ccaggcaccg gcagggcccg ggccggcggc tcacgacaac
    10741 cgcccgcccc ggctcgggcg tcctcgcggc gggccctggg agcgggcgtg agcggaacgg
    10801 ttccctccgg tgcgctcatt ggccaaaaac gtacgcaccg gatcgccgcg ccgggggcga
    10861 acgagccctc gaaggaggct cctgttcggt caactaccgc ccttccacag cgcagttgac
    10921 gggtcaggcg gcgtggcgat attcagcgag tgcctggttc cggatacggg tgcaggtgct
    10981 gctcagcaga cgcgagacgt gcatctggga gatcccgagt tccgcggcga tctgatgctg
    11041 cgtcatgtcc tggaagaacc gcatgtgcag gatgcggcac tcgcgttccg ggagggcgcg
    11101 cagggcgggc ttgaccgact cgcgatccac gaccgtgtcg aatccccgct cgggtgtgcc
    11161 gagggtgtcg gccagggccg gcccgtcacc gtcgccgccc cgggactcga cgtcgagcga
    11221 caggctgcgg taggactcca gcgcttcgag gccctcacgg acgtcctgtt cgtcgagccc
    11281 gcaccgggcg gcgaggtcgg cgatcgtggg ctcgcccggc cggacgtcct ggagatcgcg
    11341 gcgggcgacc cgcaccttgt tgcgcaactg ctgtacccgg cgcgggacat ggaccgccca
    11401 caggtggtcg cggaagtgcc gcttcagttc gccgtcgatg gtcgggatcg cgtacgcctc
    11461 gaacgcgccg cgcgcggcct cgtagcggtc gacggccttg acgagaccca tcgcggcgac
    11521 ctggtggagg tcttcgagct cggcgccgcg gttgcggtag cggcccgcga gccgccgggc
    11581 catggggagc cactgcgcca cgaggtcgtc ccgcagggcg tcgtgcgccg gacccggttc
    11641 gagcgtcgtc agacgcgcga aggcctccac cgtgtcgggg gcgtcgtcgt ggttcctcgt
    11701 acgcgtgcgg ccgggggtgg gcatggcgga actcctcggg cgtcgggtcg gactgtcacg
    11761 gcccccacag ggccccaccc cgacgacgca cccggacacg gcacgccccg ggccggaaga
    11821 acggccatgc gggcgtgcct ccgttccgaa gcacacagcg cgcctgccct gaccttcacc
    11881 acccaaacgg cccagtggcg cccgggccga caccgacgcg tccccccacc cacga
//
