LOCUS       NZ_CP032543            10798 bp    DNA     linear   CON 15-NOV-2023
DEFINITION  Streptomyces griseus subsp. griseus strain ATCC 13273 chromosome,
            complete genome.
ACCESSION   NZ_CP032543
VERSION     NZ_CP032543.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces griseus subsp. griseus
  ORGANISM  Streptomyces griseus subsp. griseus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7238194)
  AUTHORS   Liu,J., Yu,B., Liu,X. and Shen,C.
  TITLE     Complete genome sequence of Streptomyces griseus ATCC 13273
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7238194)
  AUTHORS   Liu,J., Yu,B., Liu,X. and Shen,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2018) Jiangsu Key Laboratory of Tcm Evaluation and
            Translational Research, China Pharmaceutical University, Longmian
            Avenue, Nanjing, Jiangsu 211198, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2016
            Assembly Method                   :: SMRT analysis v. 2.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 88.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/15/2023 05:04:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,408
            CDSs (total)                      :: 6,322
            Genes (coding)                    :: 5,933
            CDSs (with protein)               :: 5,933
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 299 of 389
            Pseudo Genes (incomplete)         :: 144 of 389
            Pseudo Genes (internal stop)      :: 16 of 389
            Pseudo Genes (multiple problems)  :: 65 of 389
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:22:13
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6868155
            Orig. end                         :: 6878953
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032543.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from China pharmaceutical
            University, Jiangsu Key Laboratory of TCM Evaluation and
            Translational Research.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10798
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="29"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10798
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10798
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5798](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5798)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            241..525
                     /locus_tag="D6270_RS30725"
     CDS             241..525
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="D6270_RS30725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_158650558.1"
                     /transl_table=11
                     /translation="MPSDCAAARAASRVTGPVRLRIALKLLSATQQEAAGLNLFSRSSV
                     ASATASLGHEVGELIRAEGRIVALPAPRSASFDFGDGGPEACLSARWVT"
     gene            513..872
                     /locus_tag="D6270_RS30730"
     CDS             513..872
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="D6270_RS30730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_151414741.1"
                     /transl_table=11
                     /translation="MGDLITSHVSTGAPSIEVYVQSGPPLPLGLDLDSLPEGPTPAERE
                     VGRSKILAEATGRDGTVARTLVDTPTGYRFTELSAVEIARRVLVDDHAPGFRSPVSAYG
                     PELVLAIADSHGTDL"
     gene            905..1213
                     /locus_tag="D6270_RS30735"
     CDS             905..1213
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016906428.1"
                     /locus_tag="D6270_RS30735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_225976994.1"
                     /transl_table=11
                     /translation="MSDNEAAPEHSALDAANSRALQVRALCEQVEQQVLGRTWTLPELA
                     VGFTRDTACVGRLVPAAEGTGVSGPPGVIRRGVSRVGTWSARVLRNLGSTARARDSL"
     gene            1277..1702
                     /gene="panD"
                     /locus_tag="D6270_RS30740"
     CDS             1277..1702
                     /EC_number="4.1.1.11"
                     /GO_function="GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="panD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964661.1"
                     /locus_tag="D6270_RS30740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aspartate 1-decarboxylase"
                     /protein_id="WP_109162447.1"
                     /transl_table=11
                     /translation="MMRTMFKSKIHRATVTQADLHYVGSVTVDADLMDAADLLAGELVH
                     IVDIDNGARLETYVIEGERGSGVIGINGAAAHLVHPGDLVILISYAQVEDAEARALTPK
                     VVHVDADNRIVALGSDASAPVPGSRTERSPQAVVAGG"
     gene            1745..2098
                     /locus_tag="D6270_RS30745"
     CDS             1745..2098
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018513131.1"
                     /locus_tag="D6270_RS30745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_109162446.1"
                     /transl_table=11
                     /translation="MAENAAVEISDDRERGVLTAYEDGKAAGTVAYFVMEPAPGALVAV
                     HTVVRPEYEGKGIAGALVRKFYGLAAQEGVPVVPLCPYAAKWAQRHPDEAPAAPEELVR
                     EAERQLKDHPELL"
     gene            complement(2139..2908)
                     /locus_tag="D6270_RS30750"
                     /pseudo=""
     CDS             complement(2139..2908)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PIG-L"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955646.1"
                     /locus_tag="D6270_RS30750"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="PIG-L family deacetylase"
                     /pseudo=""
                     /sec_met_domain="PIG-L (E-value: 3.6e-21, bitscore: 74.7,
                     seeds: 137, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLPSLLAVHAHPDDESIFSGGVLAQHAAAGARTAVVTATWVGGS
                     HRAAELARALEILGAGAPRLLGYADARVPESAPGRPRFLDAPLDEAVGAVVGHIRAVRP
                     RVVIAPDPYGGMTGHPDHVHTHRVTSLAVRAAGLARLFPEAGAPWQPSALYLATHPRSA
                     VEEVGRRLGRPGTPTDAFYVSDDAWITATVDVAPGSRRSWRRSSPTAARWNGEPPPAGS
                     PLCHRTFGTRYWAPSGTSARTSSPGARQTPH"
     misc_feature    2982..2999
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 19.59"
                     /tool="antismash"
     gene            complement(3573..4949)
                     /locus_tag="D6270_RS30760"
     CDS             complement(3573..4949)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_Arg_deC_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_DAP_Arg_deC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1264: diaminopimelate decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018487365.1"
                     /locus_tag="D6270_RS30760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminopimelate decarboxylase"
                     /protein_id="WP_109162444.1"
                     /sec_met_domain="Orn_Arg_deC_N (E-value: 6.8e-36, bitscore:
                     122.4, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="Orn_DAP_Arg_deC (E-value: 1e-09, bitscore:
                     37.1, seeds: 500, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGAAITDSGRTTQPPAPPIARPAVRFDAAVRAAVGQGLLSESRPV
                     AGFIHTDGVRSSVAGLREAFAHSPGVLHTFAAKASSLVPVLRLLADCGMGCEVASPGEL
                     RLAVEAGFAPGTIVLDSPAKTREEIRLALALGVAVNADSIDELRRIDTLRHRDAPSVLG
                     LRVNPQVGGGAIGPMSTATDTSKFGVALRDPGAREAVVQAFQARPWLTRLHAHVGSQGC
                     PLELIAAGVARTYELAEEINERLGTRQVTSLDIGGGLPVNFTDDTTHPTYSAYVAALTA
                     AAPGLFSGRYGLVTEFGRSLLAKNGFIGARVEYTKDAGGRRIALTHAGAQTATRTVFMP
                     DSWPLRIGTFTEDGSPRQGPALAQDIAGPCCFAGDVVAHGRELPELAEGDYVVLYDTGA
                     YYFSTPWAYNSLPRPAVYGFRSTAPAGEVVFATVREAQTMDAIAAESGLAHANALTGLG
                     "
     gene            complement(5001..5798)
                     /locus_tag="D6270_RS30765"
     CDS             complement(5001..5798)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955648.1"
                     /locus_tag="D6270_RS30765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_109162443.1"
                     /sec_met_domain="Linocin_M18 (E-value: 2.3e-88, bitscore:
                     294.1, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRELAPVTPAAWEEIEEEARRTFRRHVAGRRVVDVSDPDGP
                     TLAAVGDGHLRDIDPPTPDVVARARTSMPVIEWQVPFTVSRQAVDDVERGSADSDWQPV
                     KDAARTCAFAEDMAVIDGYAAAGITGLRDGSSHEPLPLPADARDYPVAVSQAVTRLRLA
                     GVDGPYRLLLGADAFTEAAETSDHGYPVKTHLARIVDDEILWAPAVKGGVLLSTRGGDF
                     ELCLGQDLSIGYADHDATSVHLYFQQAFTFRMLTPEAVVALIA"
     gene            complement(5798..6868)
                     /locus_tag="D6270_RS30770"
     CDS             complement(5798..6868)
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007456253.1"
                     /locus_tag="D6270_RS30770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_109162442.1"
                     /transl_table=11
                     /translation="MSAAVPDPVSVQPVVAPLTSAALFLVGTIEPGGEDAVREVLPDLA
                     AVARSLGFRYPDAGLACVTGFGSTAWDRLFAGERPAHLHPFPELRGRLHHAPSTPGDVL
                     LHIRADRMDVCYEWAAQLLTRLGGALRIVDETHGFRYLDHRDLLGFVDGTENPVGDDAR
                     AAALVGAEDPEFEGGSYVVVQKYLHDLTSWNALSVEEQERAIGRTKLNDVEFPDEEKPA
                     DSHLALNTITDPDGTERDILRANMPFGNFGQGEFGTYFIGYAADPDVTERMLRNMFLGD
                     PPGTHDRILDFSTAVTGSLFFAPRADFLDGPPPPPDQVRAGDRPEPVPAPVRQGPVAAG
                     SDHGSLRIGSLQESAQ"
     gene            complement(7145..8587)
                     /gene="gndA"
                     /locus_tag="D6270_RS30775"
     CDS             complement(7145..8587)
                     /EC_number="1.1.1.44"
                     /GO_function="GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /GO_process="GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="gndA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1100: 3-hydroxyisobutyrate dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015612845.1"
                     /locus_tag="D6270_RS30775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="WP_109162441.1"
                     /transl_table=11
                     /translation="MNGTAQIGVTGLAVMGRNLARNFARNGFTVAVHNRTTSRTDALVK
                     EFGDEGTFVAARTPEEFVAALERPRRLVIMVKAGDPTDAVIQEFAPLLEDGDVIIDGGN
                     AHFEDTRRREKELRERGIHFVGAGISGGEEGALHGPSIMPGGSAESYESLGPMLERIAA
                     KAKDGTPCTAHIGPDGAGHFVKMVHNGIEYADMQLIAEAYDLLRRVAGYSPARIADTFR
                     DWNTGRLDSYLIEITAEVLAHTDAATGKPFVDIVQDRAEQKGTGRWTVQIALDLGVPVS
                     GIAEAVFARSLSGHADLREASRELPGPVAKPLEGADASAFADRVEQALYASKIVSYTQG
                     FHQIRAGSEAYGWDIDLGSVAAIWRAGCIIRAAFLDRIRAAYGSRPELVSLLSDEEFGR
                     EIGAAQDDWRAVVAEAARQGVPTPGFAAALSYYDGLRADRLPAALTQGQRDFFGAHTYR
                     RTDREGSFHTLWGGDRSEAEAG"
     misc_feature    complement(8609..8622)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.35"
                     /tool="antismash"
     misc_feature    complement(8652..8663)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
     gene            complement(8656..9273)
                     /locus_tag="D6270_RS30780"
     CDS             complement(8656..9273)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018513126.1"
                     /locus_tag="D6270_RS30780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF5709 domain-containing protein"
                     /protein_id="WP_109162440.1"
                     /transl_table=11
                     /translation="MSDTDRGDDVYQPQEPEASDPDEQLGAEDTLLDPSGTGDPLDEGY
                     SPPDRPWVVDGLGTTAAESLTGESLEARLTRELPEAPGFTGDGVGDLAGGDGELWDGKV
                     GVARAGRLTQQAEGSVPATLTASDVGIDGAAASAEEAAMHVIPEDGDDDALLDGSLPDG
                     ALVDGASEDGGPRVDGSHADTSRVDGSHGDSSPVEGSAADGT"
     gene            9556..9696
                     /locus_tag="D6270_RS32625"
     CDS             9556..9696
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="D6270_RS32625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_158650559.1"
                     /transl_table=11
                     /translation="MGRSAEPRKSPHRAASALPPDGPPAAAPVPADAAPGTGRTALHGH
                     G"
ORIGIN
        1 gcccgcagcc cgcggcgcga tcgcggtcgg ggcagaggac cccggtgacg agatcgaggt
       61 gatcacctca ctcgggggtc ctgtggtgca agtgatgcac cacgggccct tcggtaacga
      121 gtacgagacg ctcgctcgcc tgggcgcctt cgcgcgtgaa agagggctgc gccgcgccgg
      181 tccgcaccgt gaggtcagcc ttgacccctt caccactcag acgctatggg gtgggacgtc
      241 gtgcccagcg actgcgccgc cgcccgcgcc gcttcccggg tcaccggccc cgtccggctg
      301 aggatcgccc tcaagctgct gagtgccaca cagcaggagg ccgccggcct gaacctcttc
      361 tcccgcagct ccgtcgccag cgcgacggca agcctgggac acgaggtggg cgagctgatc
      421 cgagctgagg gccggatcgt ggcgctcccg gcgcccagga gcgcctcgtt cgatttcggc
      481 gacggcggcc cggaggcgtg cctgtccgcc cgatgggtga cctgattacc agccacgtct
      541 ccaccggagc accctccatc gaggtatacg tgcagagcgg tccgccgctc ccgctcggcc
      601 tggatctcga cagcctgcct gagggaccca cgccggcgga gcgtgaggtg ggccggagca
      661 agatcctcgc cgaggcgacg gggcgcgacg gcaccgtggc ccgcaccctc gtcgacaccc
      721 cgaccggcta ccgcttcacc gagctctccg ccgtcgagat cgcccgccgg gtactcgtgg
      781 atgaccacgc ccccggcttc cggtccccgg tctccgccta cggacccgag ctcgtcctcg
      841 ccatcgccga cagccacggc accgacctgt gaacccccac ccgcacaccc aggagtcgcc
      901 acccatgagt gacaacgagg ccgcacccga gcacagcgca ctcgacgcgg cgaacagccg
      961 cgcactgcag gtccgcgctc tctgcgaaca ggtcgagcag caggtgctcg gccggacctg
     1021 gaccctgccc gagctggcgg tcgggttcac ccgcgacacc gcctgtgtcg gccggctggt
     1081 cccggcggcc gagggtacgg gggtgagtgg ccctcccggt gtcatccggc gaggcgtcag
     1141 cagagtcggc acgtggtcag cacgagtcct gaggaacctg gggagcaccg cccgggcacg
     1201 cgactcgttg tagagtgggg aaacagcact tgacctgcaa aaacgcaggc ggggagccta
     1261 gcccaggagt accgcagtga tgcgcaccat gttcaagtcc aagatccacc gggccaccgt
     1321 cacccaggcc gacctgcact acgtcggttc cgtgacggtc gacgccgacc tgatggacgc
     1381 cgccgacctg ctggccggtg agttggtcca tatcgttgac atcgacaacg gcgcccgcct
     1441 ggagacgtac gtcatcgaag gggagcgcgg ctccggggtc atcgggatca acggcgccgc
     1501 cgcacacctc gtgcaccccg gtgacctggt catcctgatc agctatgcgc aggtcgagga
     1561 cgcggaggcg cgggcgctca ccccgaaggt cgtccatgtc gacgcggaca accggatcgt
     1621 ggcgctcggg agcgatgcgt cggcgccggt gcccgggagc cgtacggagc ggagcccgca
     1681 ggcggtcgtc gcgggcggct gagtctgccg ctcgcaccct acggaacgag gagagcggaa
     1741 gcccatggcg gagaacgctg ctgtagagat cagcgacgac cgggagcgtg gcgtgctcac
     1801 ggcctacgag gacgggaagg ccgccggaac ggtcgcgtac ttcgtcatgg agcccgctcc
     1861 gggcgcgctc gtggccgtcc acacggtcgt ccggcccgag tacgagggca agggcatcgc
     1921 cggtgcgctc gtccggaagt tctacggcct cgccgctcag gagggcgtcc ccgtggtgcc
     1981 cctgtgcccg tatgcggcga agtgggcgca gcgccaccca gacgaggcgc ccgcggctcc
     2041 ggaggagctc gtacgggagg cggagcggca gctcaaggac catcccgagc tgctctgagg
     2101 agcggcggac gcccgggacc ggccgggcgt ccgccggctc acgcgctcag tgaggtgtct
     2161 gccgcgcccc gggggacgag gtcctggcgg atgtaccact cggtgcccag tacctggtgc
     2221 cgaacgtccg gtggcagagc ggcgatccgg ccgggggcgg ctccccgttc cacctcgctg
     2281 cggtgggcga ggatcgccgc cagcttctgc gggacccagg ggcgacgtcg accgttgcgg
     2341 tgatccatgc gtcgtcggag acgtagaagg cgtcggtggg ggtgcccggc cggcccaggc
     2401 gcctgcccac ctcctccacg gccgaacgcg ggtgcgtggc caggtagagg gcgctcggct
     2461 gccacggggc acccgcttcg gggaagagcc tggccagccc cgccgcccgg acggccagtg
     2521 aggtgacccg gtgggtgtgt acgtggtcgg ggtggccggt catgccgccg taggggtccg
     2581 gggcgatcac tacccgcggg cgtacggcac ggatgtggcc cacgacggcg cccaccgcct
     2641 cgtccagcgg cgcgtccagg aacctgggcc ggccaggggc ggactccgga acgcgtgcgt
     2701 ccgcgtagcc gaggagccgg ggcgctcccg cccccaggat ctccagggcc cgggccagct
     2761 cggccgcccg gtggcttccc cccacccagg tcgcggtgac cacggcggtc cgcgctccgg
     2821 ccgccgcgtg ctgggccagt acgccgcccg agaagatgga ctcgtcgtcc gggtgggcgt
     2881 ggacggcgag caggctcggc agggacatgg cgggcgcctt cctgtcggtg tacgggagag
     2941 cgggcggtgc ggtggggccg ggtgtcccac acccccgggc ccatcccgcc gtgcggatct
     3001 ccgggtctca gcgggaccgg cgggtcagcg gctcgaagcc ggcatcgtcg ctgtggcgca
     3061 cagcaggcgc ccgtcggcga aaccgtgtcc ggctgccctg gtgaccggca actcccgcag
     3121 caggatacgc gcgtgggaca tctcggccga gccggaaccc gtactccctc atgcgttgta
     3181 cgtgtggact cgacaggcgc gggcagccgc accgggagac ggtggaggaa agctccatct
     3241 gtccgtttca tgtcgttgtg gaggatcgaa atgacccacc cgtaccccga ccccgtaccg
     3301 ccgtcgccga cgcctccgcc cgtgcctggt ccgcccaccc ccgtgccgag cccgccgccc
     3361 gtgccgccgg gccccgcccc ggtgcctccc ggcccggccc cggacccgat cccgcagccc
     3421 ccggcgcccg gcccggaccc cgtaccggac ccggagccgc agcccgagcc cgccgggtcg
     3481 tagcgggagc ggagggggcg tagggaggtg cgtgggggcg gccgtcgagg tcgctcgcac
     3541 gcgcgtcccc gcgccgacgg cgggcgggag gctcagccga ggcccgtcag cgcgttcgcg
     3601 tgcgccaggc cgctctccgc ggcgatcgcg tccatcgtct gggcctcccg caccgtcgcg
     3661 aacaccacct cgccggccgg tgccgtggag cggaagccgt acaccgccgg gcgcggcagg
     3721 ctgttgtacg cccaaggggt ggagaagtag tacgccccgg tgtcgtagag gacgacgtag
     3781 tcaccctcgg ccagctccgg cagctcccgg ccgtgtgcca ccacatcgcc cgcgaagcag
     3841 cagggccccg cgatgtcctg cgccagcgcc ggtccctgtc tgggtgagcc gtcctccgtg
     3901 aacgtaccga tgcgcagcgg ccaggagtcg ggcatgaaga cggtacgggt ggcggtctgc
     3961 gcccccgcgt gggtgagtgc gatccggcgt ccgcccgcgt ccttcgtgta ctccacccgc
     4021 gccccgatga agccgttctt cgcgagcagc gaccggccga actccgtcac gagtccgtac
     4081 cggccggaga acagtcccgg agcggccgcc gtcagcgcgg ccacgtacgc ggagtaggtc
     4141 gggtgcgtgg tgtcgtccgt gaagttgacc ggcagtccgc cgccgatgtc caggctggtc
     4201 acctggcggg tgccgaggcg ttcgttgatc tcctcggcca gctcgtacgt ccgcgccacg
     4261 cccgccgcga tcagctccaa cgggcagccc tgggagccga catgggcgtg cagacgggtc
     4321 agccacgggc gcgcctggaa ggcctgtacg accgcctccc gggcacccgg gtcgcgcagc
     4381 gccacgccga acttcgaggt gtccgtggcc gtggacatcg gcccgatcgc cccgccgccc
     4441 acctgcggat tgactcgcag cccgaggacg gagggggcat cgcggtgccg cagagtgtcg
     4501 atacggcgga gttcgtcgat gctgtcggcg ttgacggcca ccccgagggc gagcgccagc
     4561 cggatctcct cgcgggtctt ggcgggggag tccaggacga tcgtccccgg cgcgaaaccg
     4621 gcctcgacgg cgagccgcag ctcacccggg ctcgccacct cgcaccccat cccgcagtcg
     4681 gcgagcagcc gcaggaccgg gacgagggag gaggccttgg ccgcgaaggt gtgcagcacc
     4741 ccggggctgt gggcgaacgc ctcccgcagc ccggcgacgg acgagcgcac cccgtccgtg
     4801 tggatgaacc cggcgaccgg ccgggactcg ctcagcagcc cctgcccgac ggccgcccgg
     4861 acggccgcgt cgaagcggac ggcggggcgt gcgatgggcg gcgcgggtgg ctgggtcgta
     4921 cggccggagt cggtgatggc tgcccccacg ggtctcggcc cttcggctct tcggcggacg
     4981 gctcgggaac ggcccgcccc tcaggcgatg agcgcgacca ccgcctccgg cgtcagcatc
     5041 cggaacgtga acgcctgctg gaagtacagg tggacgctgg tggcgtcgtg gtccgcgtag
     5101 ccgatcgaca ggtcctggcc caggcagagt tcgaagtcac cgccccgcgt cgagagcagc
     5161 accccgccct tcaccgcggg cgcccacagg atctcgtcgt ccacgatacg cgccaggtgg
     5221 gtcttgaccg gatacccgtg atcggaggtc tccgcggcct cggtgaacgc gtccgcgccg
     5281 agcagcagcc ggtacggtcc gtcgaccccg gccagccgca gccgcgtcac cgcctggctg
     5341 accgccaccg gatagtcccg ggcgtcggcg ggcagcggca gcggttcgtg cgaggagccg
     5401 tcccgcagac cggtgatccc ggctgccgcg tagccgtcga tgaccgccat gtcctcggcg
     5461 aacgcgcagg tgcgcgcggc gtccttgacg ggctgccagt cgctgtcggc ggagccgcgc
     5521 tccacatcgt ccacggcctg cctgctcacc gtgaacggca cctgccactc gatgaccggc
     5581 atcgacgtac gcgcccgggc caccacgtcc ggggtgggcg ggtcgatgtc ccggagatgc
     5641 ccgtcaccga ccgcggccag cgtcgggccg tccggatcgg agacatcgac gacccgccgg
     5701 cccgccacat ggcggcggaa cgtacgccgg gcctcctcct cgatctcttc ccaggcggcg
     5761 ggggtgaccg gtgcgagttc gcggtgcaga ttgttcatca ctgggcgctt tcttgcaggc
     5821 tgccgatgcg cagcgaaccg tggtccgacc cggcggcgac gggcccctgc cggaccggtg
     5881 cgggcaccgg ttccggccgg tccccggcac ggacctggtc gggcgggggc ggcgggccgt
     5941 cgaggaagtc ggcgcggggg gcgaagaaga gggagccggt gaccgccgtg gagaagtcga
     6001 ggatgcggtc gtgcgtgccg ggcggatcgc cgaggaacat gttgcggagc atgcgttcgg
     6061 tgacgtcggg gtcggcggcg tacccgatga agtacgtgcc gaactcgccc tggccgaagt
     6121 tgccgaaagg catgttcgcc cgcaggatgt cgcgttcggt gccgtccggg tcggtgatgg
     6181 tgttgagcgc gagatgggag tcggcgggct tctcctcgtc ggggaactcc acgtcgttca
     6241 gcttggtgcg gccgatggcg cgctcctgct cctccacgga gagcgcgttc cacgaggtca
     6301 ggtcgtgcag atatttctgt acgacgacat agctgccgcc ctcgaactcc gggtcctccg
     6361 cacccacgag cgctgccgcg cgggcgtcgt cgcccaccgg attctccgtc ccgtccacga
     6421 agcccagcag gtcgcggtgg tcgagatagc ggaaaccgtg cgtctcgtcc acgatccgca
     6481 gcgccccgcc gagcctggtg agcagctgcg ccgcccactc gtagcagacg tccatccgat
     6541 cggcccggat gtgcagcagc acatcgcccg gggtcgacgg ggcatggtgc agccggccgc
     6601 ggagctccgg gaacgggtgg aggtgggcgg ggcgctcccc ggcgaacagc cggtcccagg
     6661 cggtggagcc gaaccccgtc acacaggcga gtccggcgtc cgggtagcgg aagccgaggg
     6721 agcgggcgac ggccgccagg tccggcagca cctctcgcac cgcgtcctcc ccacccggct
     6781 cgatcgtgcc gaccaggaag agcgccgcgc tggtcagcgg ggccacgacg ggctggacgg
     6841 agacggggtc ggggacggct gcggacatgc gcggcactcc aggcgtcgga tggatcgtgc
     6901 gcgttcgcgt gggatcgcgt gcacagggct cgtagcccac taagccatgc cctgcggacg
     6961 cccgcacccg gggtggcccg gccggctgac accgtccggg ccgccccagg cgcaggccga
     7021 ctgggagggt cccggggtgg cctcggccgg ctgggaccgt gccggggttg gtcccgccga
     7081 ctcgcaccgc cccgtgcgcc gccccctcag ggctcgcccc gcccccgcct ccggcgcgtg
     7141 cgactcagcc ggcctcggcc tcggaccggt ccccgcccca cagcgtgtgg aacgaaccct
     7201 cccggtccgt acgccgatac gtgtgtgccc cgaagaagtc ccgctgcccc tgcgtgagcg
     7261 ccgcgggcag ccggtccgcg cgcagcccgt cgtagtagga gagcgcggcg gcgaaacccg
     7321 gcgtcggcac accctggcgc gccgcctccg ccaccaccgc ccgccagtcg tcctgcgcgg
     7381 ccccgatctc ccgcccgaac tcctcgtcgg acagcaggct caccagctcg ggccgggaac
     7441 cgtaggcggc tctgatccgg tccaggaacg ccgcccggat gatgcagccc gcccgccaga
     7501 tcgcggccac ggaaccgagg tcgatgtccc agccgtacgc ctcgctgccg gcccggatct
     7561 ggtggaagcc ctgcgtgtac gagacgatct tcgaggcgta cagggcctgt tcgacccggt
     7621 ccgcgaacgc cgacgcgtcc gccccctcca gcggcttcgc caccgggccc ggcagctccc
     7681 gtgacgcctc gcgcaggtcc gcgtggcccg acagcgagcg ggcgaagacc gcctcggcga
     7741 tcccggagac cggaacgccc aggtccagcg cgatctgcac ggtccaccgc ccggtgccct
     7801 tctgttccgc ccggtcctgc acgatgtcga cgaacggctt gccggtggcc gcgtccgtgt
     7861 gcgccaggac ctcggcggtg atctcgatca gatacgaatc cagccgcccc gtgttccagt
     7921 cgcggaacgt gtcggcgatc cgcgcggggg agtacccggc gacccggcgc agcaggtcgt
     7981 acgcctccgc gatgagctgc atgtccgcgt actcgatgcc gttgtgcacc atcttcacga
     8041 agtgcccggc gccgtccggg ccgatgtgcg cggtgcacgg ggtgccgtcc ttcgccttgg
     8101 cggcgatcct ctccagcatc gggcccagcg actcgtacga ctcggccgat ccgcccggca
     8161 tgatgctcgg gccgtggagc gcgccctcct cgccgccgga gatgcccgca ccgacgaagt
     8221 ggatcccgcg ctcgcgcagc tccttctccc ggcggcgggt gtcctcgaag tgggcgttgc
     8281 cgccgtcgat gatgacgtcg ccgtcctcca ggagcggcgc gaactcctgg atcaccgcgt
     8341 ccgtggggtc cccggccttg accatgatca ccaggcgcct gggccgctcc agagccgcga
     8401 cgaactcctc gggggtgcgc gcggcgacga acgtgccctc gtcgccgaac tccttcacca
     8461 gggcgtcggt gcgggaggtc gtgcggttgt gcacggcgac ggtgaaaccg ttgcgggcga
     8521 agttccgggc caggttgcgg cccatgaccg cgagtccggt gacgccgatc tgggcggtgc
     8581 cgttcatctg tgtgctcctg cgcttccggg gacgttcgtt cggtcagggg ggtacgggac
     8641 gggggacggc cgcgttcacg tgccgtcggc ggcggagcct tcgacggggg agctgtcgcc
     8701 atgagagccg tcgacccggg aggtgtcggc atgagaaccg tccacacggg ggccgccgtc
     8761 ctcggaagcc ccgtcgacga gcgcgccgtc cggcagcgaa ccgtccagca gggcgtcgtc
     8821 gtccccgtcc tcggggatca catgcatcgc ggcctcctcg gccgacgccg ccgcgccgtc
     8881 gatgcccaca tcgctggcgg tgagcgtggc cgggacgctg ccctccgcct gctgggtcag
     8941 ccgcccggcg cgggcgacgc cgaccttgcc gtcccagagc tcgccgtccc cgcccgccag
     9001 gtctcccacg ccgtcgccgg tgaacccggg cgcctcggga agttccctgg tcagccgcgc
     9061 ctccagcgac tcgcccgtga ggctttcggc ggctgtggtg ccgagtccat cgaccaccca
     9121 gggccggtcc ggcggtgagt agccctcgtc gagggggtcg ccggtgcctg aggggtccag
     9181 cagcgtgtcc tcggcgccga gctgttcatc ggggtcggaa gcctccggct cctggggctg
     9241 gtagacgtcg tccccccggt cggtgtcgct catggcgcgt cctttcctcg ggagagaacc
     9301 actgtcccct gtacgcaatt ccactccggt accgctccgg ccgcaaaggg aagatcggaa
     9361 tccggcactc cgccgtggcg cgcccaccgg gtccgcacgg aacccgcacc cctgctcctc
     9421 ccgcccgccg acggccctga cgcgccttga ggggccctgg tccggcgcga ggacgtggcc
     9481 cgctccgccc cgagggggtg ccccggtccg gcccgagcgg accggggcac gtcgggccgg
     9541 acgtacactc ggctcatggg tcgtagcgcc gaaccgagga agagcccgca ccgggccgcc
     9601 tccgcgctgc ccccggacgg gccccccgcc gccgcaccgg tgcccgccga cgcggcgccc
     9661 ggaacgggcc gtaccgcgct gcacggccac ggctagcggt cttcggcctc cgcggacccg
     9721 ggccgacacc ccggtccgtc gcccctccca cgaacgactc cggcctctcc gcgccggagc
     9781 gagccgccgc ccccggcccg cgccggatgc gcgatcggcc cccggctcgg cacaccgatc
     9841 acgcgcacca cacctgactg attacgtcac gcatcacgca tcacgcatca cgcatcacgc
     9901 atcacgcatc acgcggcaca cggcgcctcc gcccgtgccg ccgcccggcg acggccgctg
     9961 agacgggccg agccgcactc gaagggacca ccgtgaagct gagcgaccta ctggccgggc
    10021 aggaccacca cgtactgcag ggaagtcccg agcgcacgcg gatcaccgcc ggtacgacgt
    10081 tcgacgcgga ccgggtcacc cccggctcgg tcttcgtggc ggtccccggc cacgcggccg
    10141 gcggtcccga cgccgtcgca ccggccgtcg cccgcggcgc ggcagccgtc gtcgtcgacg
    10201 cccaggcccc acctctcccc gcgtcggcgg gcgacgtgtg cgcgatccgg gtgccggacg
    10261 cccgcaaggc cgcggccgtc atcgcctcgc gctacttcgg tgagccgggc cgtgcgatgg
    10321 acctgatcgc gatcaccggg accaacggca agacctccgt ctcgtacatg gtcgagtcgg
    10381 tgctgcggct cgccgagggc gcccgggtcg gggtgatcgg cacggcgggg agccggatcg
    10441 gggacgaacc gatcccgatg ccgccctccg cgctctccac ccccgaatcg cccgacctcc
    10501 agtacctgct gagccggatg cgggacagcg cggccggcag cgtggtcctg gaagccacct
    10561 cgatgggcct gctgacccac cgtctggacc ggaccttcgt cgacgtgggc gtcttcacca
    10621 atctgtcgca ggaccacctc gacgaccacg gcaccatgga gcactaccgg gacgccaaac
    10681 tccgcctctt ccaggggctg tgccggcgcg cggtcatcaa cgcggacgac ccggtcggtg
    10741 ccggcatccg ggagctgatg cccgacgctg tgaccacgta cgcgctggac ggcccggc
//
