LOCUS       NZ_CP032543            11326 bp    DNA     linear   CON 15-NOV-2023
DEFINITION  Streptomyces griseus subsp. griseus strain ATCC 13273 chromosome,
            complete genome.
ACCESSION   NZ_CP032543
VERSION     NZ_CP032543.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces griseus subsp. griseus
  ORGANISM  Streptomyces griseus subsp. griseus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7238194)
  AUTHORS   Liu,J., Yu,B., Liu,X. and Shen,C.
  TITLE     Complete genome sequence of Streptomyces griseus ATCC 13273
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7238194)
  AUTHORS   Liu,J., Yu,B., Liu,X. and Shen,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2018) Jiangsu Key Laboratory of Tcm Evaluation and
            Translational Research, China Pharmaceutical University, Longmian
            Avenue, Nanjing, Jiangsu 211198, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2016
            Assembly Method                   :: SMRT analysis v. 2.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 88.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/15/2023 05:04:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,408
            CDSs (total)                      :: 6,322
            Genes (coding)                    :: 5,933
            CDSs (with protein)               :: 5,933
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 299 of 389
            Pseudo Genes (incomplete)         :: 144 of 389
            Pseudo Genes (internal stop)      :: 16 of 389
            Pseudo Genes (multiple problems)  :: 65 of 389
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:22:13
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6065548
            Orig. end                         :: 6076874
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032543.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from China pharmaceutical
            University, Jiangsu Key Laboratory of TCM Evaluation and
            Translational Research.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11326
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="23"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11326
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11326
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6326](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6326)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(650..1522)
                     /locus_tag="D6270_RS27220"
     CDS             complement(650..1522)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019356589.1"
                     /locus_tag="D6270_RS27220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="WP_109163055.1"
                     /transl_table=11
                     /translation="MKLLVHGAGVCGSLLAAQLHEAGADVSLLARGRRLESLRRHGVRL
                     AEEDSPVVRQVPVPVVEHAADGYDLAAVTVRTHQVDAVLESLAGFGGDVLFLHNWAAGA
                     QPLEEVVGRGRVLLGFPAVGGTMDGDVVRHRASNPVTDRVAMPIGEPDGRTTPRLERIV
                     RVFRAAGVNTKAEPRMDAWLKTHAAFSVPLGRAAYTAGGPAALAEDSDAVRAMLRLMRQ
                     NLAALPTPPVPRPFGALDKVPEGVLVPLLRRFLRSRTAVRSGLSDTSPATAAELDRLAG
                     QLRALTQAG"
     misc_feature    1516..1532
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.81"
                     /tool="antismash"
     misc_feature    1543..1556
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: strong, score: 23.98"
                     /tool="antismash"
     gene            1583..2215
                     /locus_tag="D6270_RS27225"
     CDS             1583..2215
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049265.1"
                     /locus_tag="D6270_RS27225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR family transcriptional regulator"
                     /protein_id="WP_109163054.1"
                     /transl_table=11
                     /translation="MVLPDTRSNVLDAAERLFAENGYRGTSVRAITNLAGANLAAVAYH
                     FGSKAELMAAVARRVAGPINRAQHARLDEALAKNADPDVADLVEAFVGPLFDEMPADDA
                     DGARTSRLIMTILSDPAEEARSWTGPDEAAVRERFLSAFTRALPGVAPQELWFRLRGVL
                     AVTAVDRVEVHNQPTPGCSTAAGEDAKRWAITFLTAAMSAPATETVR"
     misc_feature    1623..1638
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.58"
                     /tool="antismash"
     gene            complement(2285..3850)
                     /locus_tag="D6270_RS27230"
     CDS             complement(2285..3850)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (104-471). E-value:
                     3.3e-17. Score: 54.8. Matches aSDomain:
                     nrpspksdomains_D6270_RS27230_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956907.1"
                     /locus_tag="D6270_RS27230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_109167254.1"
                     /sec_met_domain="PF00561 (E-value: 4.4e-22, bitscore: 77.5,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAVPLYATIGSLVLSALVAAPATASDGPAPASAEERGTTVAAVR
                     AAAAGISFGACPEEEQLPDTLECGTVGVPLDYAEPDGRTIELTVSRALATGDAAGRQGS
                     LVYNPGGPGASSVTFPLMGEMSEWKEIAEAYDMVGYAPRGVGASAPLSCQDPAAFAQSP
                     TDSPVHPTAAYKKQRIAQARAYARGCEKHAGEALRHYTSLNNARDLEVVRAALGDERLT
                     FMGASYGTYLGSLYATLFPSRVRRMVFDSAVDPDPEQIWYRSNLDQSLAFESRWEDFRR
                     WVARHDDVYRLGTTPEAVQRHYDDVRTRLAAEPAGGKVGPGQFHAAFLQAGYYDDYWAM
                     RATALSEYVRGNPEPLITQMSPRAITAKGDENARAVYTAIECNDAPWPEEWEVWDRDHS
                     DLATVAPFQTWDNAFTNLPCAFWPAPRQQPLDVSTGWGELPPVLILAAERDAATPYKGA
                     LELQRRLRGAVLVTERDAGTHGIGGAGNTCVDDHLRRYLLTGATPDGDAECAPHAEPNP
                     LSLD"
     aSDomain        complement(2438..3538)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_D6270_RS27230_Abhydrolase_1.1"
                     /evalue="3.30E-17"
                     /label="D6270_RS27230_Abhydrolase_1.1"
                     /locus_tag="D6270_RS27230"
                     /protein_end="471"
                     /protein_start="104"
                     /score="54.8"
                     /tool="antismash"
                     /translation="LVYNPGGPGASSVTFPLMGEMSEWKEIAEAYDMVGYAPRGVGASA
                     PLSCQDPAAFAQSPTDSPVHPTAAYKKQRIAQARAYARGCEKHAGEALRHYTSLNNARD
                     LEVVRAALGDERLTFMGASYGTYLGSLYATLFPSRVRRMVFDSAVDPDPEQIWYRSNLD
                     QSLAFESRWEDFRRWVARHDDVYRLGTTPEAVQRHYDDVRTRLAAEPAGGKVGPGQFHA
                     AFLQAGYYDDYWAMRATALSEYVRGNPEPLITQMSPRAITAKGDENARAVYTAIECNDA
                     PWPEEWEVWDRDHSDLATVAPFQTWDNAFTNLPCAFWPAPRQQPLDVSTGWGELPPVLI
                     LAAERDAATPYKGALELQRRLRGAVLV"
     gene            complement(3973..4995)
                     /locus_tag="D6270_RS27235"
     CDS             complement(3973..4995)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018514514.1"
                     /locus_tag="D6270_RS27235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_204117046.1"
                     /transl_table=11
                     /translation="MNTLALVMNLVAALSTVLLLAKVAVSRSRRPSADPSATVHDLYEA
                     AFLNGGPARVADTALTALYTDGRILIGGPGIISVQRAQANDPVERAVFQALSTSPSGAL
                     HAVREAVMHHPAVQEIGDGLAQRGLLVRPADGRPVRRWGLIQGVSCFVAFPLSILLTVL
                     QYIAHDSPFDMPFPFVVKVLPGILLGGISGLVVAASGAKRITRAGRRAAQAFRAANAYL
                     LTPPYLVANRGLHALPDPVLQAQLIAAAQQHLRTRRSRSGFSGGRSAGYVGGAAASGFA
                     ATTVWCADSGPGHTSCGGGGGCGGGSGCGGSGSGCGSSGSSCGGSSSSCGGGSSCGSSS
                     "
     gene            complement(5001..6326)
                     /locus_tag="D6270_RS27240"
     CDS             complement(5001..6326)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018100703.1"
                     /locus_tag="D6270_RS27240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_109163053.1"
                     /sec_met_domain="DUF692 (E-value: 1.9e-92, bitscore: 307.9,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLGIGIGWRPEIAAEVEALTGIDWVEAVAENLCADHLPDSLVRL
                     RERGVTVVPHGVSLGLGGADRPDAGRLAGLAARAELLGTPLVTEHIAFVRAGGPLTASA
                     RLEAGHLLPVPRTWDALEVLCENVRIAQDSLPVPLALENIAALVSWPDEELTEGQFLAE
                     LVDRTGVRLLIDVANLHTNHVNLGQDPAKALDELPVEAIAYVHVAGGVEKDGVWHDTHA
                     HPVTAPVLDVLAELRSRVDPPGVLLERDDAFPPGAELAGELDTIRETLRSAATGSRRAV
                     PRTATSLACEAAPRTETPSAPTAAPGARDRTAVAQTVLLSALVAGTPAPEGFDHRRLRV
                     QSRALAAKRADVVAKVAPELPEILGDGYRAAFLDYAEGRPMSAGYRRDALGFVERILVS
                     GGLPDPVAQRRLTHWWEDRSGPRPPSRTTRIVRAARAVLAGK"
     gene            complement(6504..7250)
                     /locus_tag="D6270_RS27245"
     CDS             complement(6504..7250)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072530.1"
                     /locus_tag="D6270_RS27245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_202419124.1"
                     /transl_table=11
                     /translation="MRRINGTALIIAALVATVGALAFPVWSYADRSGTGEANLNASSVA
                     TQWGPLSATDRDFLVKVRLAGLWELPAGQQAIERAPSEATKAAGDHLVVGHTDLDRRAR
                     DVAAKLGVELPNQPTEQQRAWLAELTAASGDEYERKFANILRAAHGKVFGLIGQVRHTT
                     RNTLIRQLASDANQTVLDHITMLEGTGFVDFDALAREAAGGSTASPSGPSMPQGGQVPQ
                     VPVPVTPTGDQSFTSRPVPPTMPPLP"
     gene            complement(7482..8249)
                     /locus_tag="D6270_RS27250"
     CDS             complement(7482..8249)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019993147.1"
                     /locus_tag="D6270_RS27250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_109163051.1"
                     /transl_table=11
                     /translation="MSSDDTSQNTSSRTTVSPSPFHNGPTDRDEAPQYVLPLVVHLEKT
                     APPARTDALRTAARAVLTILSDERSLGEGEWAGAMRDWEDARIRKVVRRARGAEWRKAA
                     ALPGITVTGTGAEVRVYPPIPLDGWPKELAKLQVSGTDLDDPEPPAAPDPEGPVLWLNP
                     ELEMSAGKTMAQVGHGAQLAWWELSQTERKAWREAGFPLSVATATPARWRELTTSGLPV
                     VRDAGFTEIAPGPTVAVEGGRRFCPLPGVRLRR"
     gene            8322..8996
                     /locus_tag="D6270_RS27255"
     CDS             8322..8996
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378552.1"
                     /locus_tag="D6270_RS27255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AIM24 family protein"
                     /protein_id="WP_109163050.1"
                     /transl_table=11
                     /translation="MKSDLFSSEHMAQPATAPGMTLQNPKSIKYAVNGEMHARQGSMIA
                     FRGNLQFERKGQGIGGLLKRAVTGEGLALMAVRGQGEAWFAHEAANCFILEMDQGDQIT
                     INGRNVLCFDATLSYEIKTVKGAGMVGGGLFNSVFSGYGKLGLMCEGTPIVIPVTGAQP
                     VYVDTDAVVGWSQQLTTSLHRSQSVGSMVRGGSGEAVQLMLQGEGFVIVRPSEARPERT
                     AN"
     gene            9063..9245
                     /locus_tag="D6270_RS27260"
     CDS             9063..9245
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049036.1"
                     /locus_tag="D6270_RS27260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_109163049.1"
                     /transl_table=11
                     /translation="MDAAAYCETPAPVTTEADAGPPFAECVLCREPTEYPESAKGATLC
                     PVCAWQEAGRSACSG"
     gene            complement(9688..10422)
                     /locus_tag="D6270_RS27265"
     CDS             complement(9688..10422)
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019766594.1"
                     /locus_tag="D6270_RS27265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_225977084.1"
                     /transl_table=11
                     /translation="MAPGPAGRTPVFERRPAGGAAAAGGGGTAGGAAAEKVVALTFDAD
                     MTADQGPRAAAGERFDNPELIALLRRLKVPSTVFMTGRWAEEYPAQAKGIGTDDLFEVA
                     NHSYSHHAFTADCYGLPVVAKESMARDVERAFSAFREAGVRNVVPYFRFPGGCYDDAAL
                     GALAPANVTAVQWDVVSGDAFATDAGAVAEQVLDGVRPGSLVVLHCTRSAAPVTAEAIS
                     RIVPELRKRGYRFVKVSELMEG"
ORIGIN
        1 tggtcgaaca tggccctgca ccggcccgcc gagttcaaca agtcgcacgt tccggcgttc
       61 ctcgccgacg agacgccccg cgactacatc agcgtctacc ccttcgtacg gtcctacgac
      121 tggtacctgc tgcccgacga ggaccgccgc cgcatgctcg cggaccacgg caagatggcc
      181 cggggctacc ccgacgtccg cgccaacacc gtcgcctcgt tctcgctcgg cgactacgag
      241 tgggtcctcg ccttcgaggc ggacgagctg taccgcatcg tcgacctgat gcgccacctg
      301 cgcgcctccg aggcacggct ccacgtgcgc gaggaggtcc cgttctacac gggccgcagg
      361 aggagcgtcg cagacctggt ggccgggctc gcatagccag cgagttcccg caccacccca
      421 acccggaaga tcagaagacg ggcaccaggc accgaccgtg ccgcccgccg ttccagcgac
      481 accgggttcg gcttcgggcc ctgtcggagt gcacccgctg cactccgaca gggcccgtcc
      541 gcgctccagg ttcggttgtc gacgcggccg acgggtggcc ggccgtcgag gccgtgcgcc
      601 ggtgacggtc gcggggcgaa ggaaccggaa cggcccggtg gctcggggcc tagccggctt
      661 gcgtgagcgc gcgcagttgt ccggccagcc ggtccagctc ggcggccgtc gcgggagagg
      721 tgtcgctgag tccgctgcgg acggcggtcc ggctgcgcag gaagcgcctc agcaggggca
      781 cgagcacccc ttccggcact ttgtccagtg cgccgaacgg tcgcgggacc ggcggtgtcg
      841 gcagggcggc caggttctgg cgcatgaggc ggagcatcgc gcgcacggcg tcggaatcct
      901 cggccagagc cgccgggccg cccgccgtgt acgcggcccg gccgagcggc accgagaacg
      961 cggcgtgggt cttgagccag gcgtccatcc ggggctcggc cttcgtattg acgccggcgg
     1021 cccggaagac gcggacgatc cgttccagcc gcggggtggt gcgcccgtcg ggctcaccga
     1081 tcggcatggc gacccggtcg gtcacggggt tgctcgcgcg gtggcggacc acgtcgccgt
     1141 ccatggtgcc gccgacggca gggaagccga gcagcacgcg cccccggccg accacctctt
     1201 ccagcggctg tgcgccggcc gcccagttgt gcaggaacag cacatcgccg ccgaagccgg
     1261 ccagtgactc cagcaccgcg tccacctgat gggtgcggac ggtgacggcg gccaggtcgt
     1321 acccgtccgc cgcgtgctcg accaccggga ccggcacctg ccggacgacg gggctgtcct
     1381 cctcggcgag tcgcacaccg tgccgccgca gggactccag ccgtcggccc ctggccagca
     1441 gcgagacgtc ggccccggcc tcgtggagct gggccgcgag caggctgccg cacaccccgg
     1501 cgccatggac gagcagtttc atgcgatcac atccgcctaa gtcaaacgtt cgtttcaatc
     1561 agtcgtctgt acagtacggc acatggtgct tcccgatacc cggagcaacg ttctcgacgc
     1621 tgccgaacgc ctcttcgccg agaacggcta ccgcgggacc tcggtccgcg cgatcaccaa
     1681 cctcgccggg gccaacctgg ccgctgtggc ctatcacttc gggtcgaagg cggaactcat
     1741 ggcggcggtg gcccgccgcg tcgccgggcc catcaacagg gcccagcacg cccggctcga
     1801 cgaggctctc gcgaagaacg cggacccgga cgtcgccgac ctcgtggaag ccttcgtcgg
     1861 ccccctgttc gacgagatgc ccgccgacga cgccgacgga gcccgcacgt cccggctgat
     1921 catgacgatc ctcagcgatc cggccgaaga ggcccgcagc tggaccggac cggacgaagc
     1981 cgccgtccgt gagcggttcc tgtcggcctt cacccgcgca ctgccgggcg ttgccccgca
     2041 ggagctgtgg ttccggctgc ggggagtcct cgccgtgacg gccgtggacc gcgtcgaggt
     2101 ccacaaccag ccgacccccg gctgctccac ggcagcgggg gaggatgcca agcgctgggc
     2161 gatcacgttc ctgacggcgg cgatgagcgc cccggcgacc gaaaccgttc gctaggccgt
     2221 ttcgccgccc gcgttccggg ccacggcggg ccggacacgg cgcacgccct cgccgccggt
     2281 acggctagtc cagcgagagg gggttcggct ccgcgtgcgg ggcgcactcc gcgtccccgt
     2341 ccggtgtcgc ccccgtcagc aggtagcgcc gcagatggtc gtccacgcag gtgttcccgg
     2401 ccccgccgat gccgtgcgtc cccgcgtccc gctcggtcac gagcaccgcg ccgcgcagcc
     2461 gccgctgaag ttccagcgcc cccttgtacg gggtcgccgc atcgcgctcc gccgccagga
     2521 tcagcaccgg cggcagctcg ccccacccgg tggagacgtc cagcggctgc tgccggggcg
     2581 cgggccagaa ggcgcacggc aggttggtga aggcgttgtc ccaggtctgg aagggggcga
     2641 cggtcgcgag gtcgctgtgg tcgcggtccc agacctccca ctcctcgggc catggggcgt
     2701 cgttgcactc gatcgcggtg tagacggccc gcgcgttctc gtcccccttg gccgtgatcg
     2761 cgcgcggcga catctgggtg atcagcggct ccgggttccc ccggacgtac tcggagaggg
     2821 cggtcgcccg catcgcccag tagtcgtcgt agtaaccggc ctggaggaac gccgcgtgga
     2881 actggccggg ccccaccttc ccgcccgccg gttcggccgc gaggcgggtg cgcacgtcgt
     2941 cgtaatgacg ctgcacggcc tcgggagtgg tgccgagccg gtagacgtcg tcgtgcctgg
     3001 cgacccagcg ccggaagtcc tcccagcggg actcgaaggc cagcgactgg tcgaggttgg
     3061 agcggtacca gatctgctcc gggtccgggt cgaccgcgga atcgaagacc atacggcgca
     3121 cgcgtgaggg aaagagggtc gcgtacaagg aacccagata cgtgccgtac gaggcaccca
     3181 tgaacgtcag ccgctcgtcc ccgagcgccg cccgcaccac ctccaggtca cgcgcgttgt
     3241 tgagcgaggt gtagtggcgc agggcctcgc ccgcgtgctt ctcgcacccc cgcgcgtacg
     3301 cccgcgcctg cgcgatccgc tgcttcttgt acgcggccgt ggggtgcacg ggggagtcgg
     3361 tggggctctg cgcgaaggcc gcggggtcct ggcaggagag cggggcggag gcgccgacac
     3421 cgcgcggggc gtagccgacc atgtcgtacg cctcggcgat ctccttccac tcggacatct
     3481 cgcccatcag cgggaaggtg acgctggagg cgccggggcc gcccgggttg tagacgaggg
     3541 agccctgccg gccggccgca tcgccggtgg ccagggcgcg gctgacggtg agttcgatgg
     3601 tccggccgtc gggctccgcg tagtccaggg ggacgccgac ggtgccgcac tccagggtgt
     3661 ccggcagctg ctcctcctcc gggcaggcgc cgaaggagat cccggcggct gccgcgcgga
     3721 ccgccgccac cgtcgtaccg cgctcctcgg cggaggccgg agcggggccg tccgaggcgg
     3781 tggcaggggc ggccaccagg gcggacagga ccagggagcc gatcgttgcg tacagcggta
     3841 ctgctctcac ggcgggacct ctctgcggat gcggagaggg gatggtgagg tgcggagcgg
     3901 gtgaagtcca gcaccgggcc ggtgtgttgc ggggaacgac ccggttgccg cactccttct
     3961 cccggtggcg gttcaggaac tgctgccgca gctggagccg ccaccacaac tggagcttga
     4021 ccccccgcag ctggaccccg agctcccgca gccggacccc gaaccgccgc acccggaccc
     4081 accgccgcag ccgcctccgc ccccgcaact cgtatgcccc gggcccgagt cggcgcacca
     4141 caccgtggtc gccgcgaagc ccgacgccgc cgcacctccc acgtaccccg ccgaccggcc
     4201 ccccgagaag ccggaccgtg agcgccgggt ccgcaggtgc tgctgggccg ccgcgatcag
     4261 ctgggcctgg agcaccgggt cggggagagc gtgcagcccc cggttggcga cgaggtacgg
     4321 cggggtcagc aggtaggcgt tcgcggctcg aaaggcctgc gcggcccggc ggcccgcccg
     4381 ggtgatccgc ttggcgccgg acgcggccac gacgagcccg ctgatcccgc ccagcaggat
     4441 gccgggcagc accttcacga cgaaggggaa cggcatgtcg aacggggagt cgtgtgcgat
     4501 gtactgaagg accgtgagca ggatggagag cgggaacgcg acgaagcagc tcaccccctg
     4561 gatcagtccc caccgccgca cgggacggcc gtcggcgggc cggacgagca gcccgcgctg
     4621 agccagcccg tcgccgatct cctgcaccgc cgggtggtgc atcaccgcct cccgaaccgc
     4681 gtgcagagcc ccgctcggtg aggtcgacag cgcctggaac acggcgcgtt cgaccgggtc
     4741 gttcgcctgc gcacgctgta cggagatgat gccggggccg ccgatgagga tccggccgtc
     4801 ggtgtacagg gcggtgagcg cggtgtccgc gaccctggcc ggaccgccgt tcaggaacgc
     4861 cgcctcgtag aggtcgtgga cggtggcgga ggggtcggcg gacgggcggc gggaccggga
     4921 gacggccacc ttcgccagga gcagcacggt ggagagggcg gccacgaggt tcattaccag
     4981 ggcgagcgtg ttcacggcgg tcacttcccc gcgaggacgg cacgggccgc ccggacgata
     5041 cgggtggtcc ggctcggtgg ccggggcccg gagcggtcct cccaccagtg cgtcagcctg
     5101 cgctgagcca cggggtcggg aaggccgccc gagaccagga tccgctccac gaaaccgagg
     5161 gcgtcgcgcc ggtaaccggc gctcatcggg cggccctcgg cgtagtcgag gaacgcggcg
     5221 cggtagccgt cgccgaggat ctccggcagc tcgggcgcca ccttcgcgac gacatcggcg
     5281 cgcttggcgg ccagcgcccg gctctgcacc cgcagccgcc ggtggtcgaa gccctcgggc
     5341 gccggggtgc ccgcgacgag cgcggagagc agtacggtct gggcgacggc ggtacggtcg
     5401 cgggctccgg gggcggcggt gggcgccgag ggcgtctccg tacgaggggc ggcttcgcac
     5461 gccagggacg tcgccgtgcg ggggacggcc cggcgtgagc cggtcgccgc ggaccgcagc
     5521 gtctcccgga tcgtgtccag ctcgcccgcc agctccgcgc cgggcgggaa ggcgtcgtcg
     5581 cgctccagca gcacgcccgg cgggtcgacc cgcgagcgca gctcggccag tacgtcgagg
     5641 accggggcgg tcaccgggtg ggcgtgcgtg tcgtgccaga cgccgtcctt ctcgaccccg
     5701 cccgccacat ggacgtacgc gatggcctcc accggcagct cgtccagcgc cttcgccggg
     5761 tcctggccga ggttgacgtg gttggtgtgc aggttggcca cgtcgatcag cagccgcacg
     5821 ccggtccggt cgaccagctc cgccaggaac tgcccctcgg tcagctcctc gtccggccag
     5881 gagaccagcg ccgcgatgtt ctccagggcc agcggcacgg gcagcgagtc ctgggcgatg
     5941 cgcacgttct cgcagagcac ctccagcgcg tcccaggtgc gcggtacggg cagcaggtgg
     6001 cccgcctcca gccgcgcgga cgcggtgagc ggccccccgg cccgtacgaa cgcgatgtgc
     6061 tcggtcacca gcggtgtacc cagcagctcg gcccgcgccg ccagccccgc gagccgcccg
     6121 gcgtccgggc ggtccgcgcc gcccaggccg agcgagacgc cgtgcgggac gactgtcacc
     6181 ccccgttccc gcaggcgcac tagggagtcc gggaggtggt cggcgcagag gttctccgcc
     6241 accgcctcca cccagtcgat ccccgtgagc gcctccacct cggccgcgat ctccggccgc
     6301 cagccgatcc cgatgcccag cttcatggtg tccccctcct ccgctccctg tccgggtgat
     6361 ggccccggga agcggtgccc aacccgaaca ggggacgttc agaggaggat ttgaggttcc
     6421 gggcgggcgg gtctcgttac ggcatcggct tggagggctg tcctgcggga gcggcttcgg
     6481 aggccgcgtc agggtggaag gccctacggc agcggcggca tcgtcggcgg taccgggcgc
     6541 gaggtgaacg actggtcgcc ggtcggtgtc accggcaccg gaacctgcgg cacctgcccg
     6601 ccctgcggca tcgacgggcc cgaggggctg gccgtggacc cgccggccgc ctcacgcgcc
     6661 agggcgtcga agtcgacgaa gccggtgccc tccagcatgg tgatgtggtc cagcacggtc
     6721 tggttcgcgt cgctggcgag ctgccggatc agggtgttgc gggtggtgtg ccggacctgg
     6781 cctatcagcc cgaacacctt gccgtgcgcg gcccgcagga tgttggcgaa cttccgctcg
     6841 tactcgtccc cgctcgccgc cgtcagctcc gcgagccacg cccgctgctg ctcggtcggc
     6901 tggttgggca gctccacccc gagcttcgcg gccacgtccc gggcccgccg gtccaggtcc
     6961 gtgtggccga ccaccagatg gtccccggcc gccttcgtcg cctcgctggg cgcccgttcg
     7021 atcgcctgct gaccggcggg cagctcccac agcccggcga gacgcacctt gaccaggaag
     7081 tcccggtcgg tcgcggagag cgggccccac tgggtggcga cgctggacgc gttcaggttc
     7141 gcctcgccgg tgcccgaacg gtccgcgtag gaccagacgg ggaaggcgag cgcgcccact
     7201 gtggcgacca gtgccgcgat gatgagggcc gttccgttga tgcgtcgcaa caaggtgtct
     7261 cccgggacga ggcgaggcgt gacagcgccg gtcagtcgat caggcgtcaa tcaaccgccg
     7321 accggctcgc cgcgcaggct acttgtcggt aatgccggtg gggcagtatc gggagatacg
     7381 tagtcgggcg caggaatact cagccgcggg cgggccggtc ccggcctacg ggggcgccgc
     7441 gccgtggagg gcggccggcg gatctccgag ggggcgtgcg ttcagcggcg gaggcgtacg
     7501 cccgggaggg gacagaaccg ccggccgccc tccacggcga cggtgggccc gggggcgatc
     7561 tccgtgaacc ccgcgtcgcg caccaccggc aggccgctcg tggtcagctc gcgccagcgc
     7621 gccggggtgg ccgtggccac ggagagcggg aaccccgcct cgcgccaggc cttgcgctcg
     7681 gtctgtgaca gctcccacca ggcgagctgg gcgccgtggc cgacctgggc catcgtcttg
     7741 cccgccgaca tctccagctc cgggttgagc cacagcaccg gcccctccgg gtcgggggcg
     7801 gcgggcggct cggggtcgtc caggtcggtg cccgacacct ggagcttggc cagctccttg
     7861 ggccagccgt ccagcgggat cggcgggtag acccggacct ccgcgccggt gcccgtcacc
     7921 gtgatcccgg gcagcgcggc cgccttgcgc cactccgccc cacgtgcccg gcgcaccacc
     7981 ttgcggatcc gggcgtcctc ccagtcccgc atcgcccccg cccactcgcc ctcgccgagg
     8041 gaccgctcgt cggacaggat cgtcagcacc gcgcgggcgg cggtgcgcag ggcgtccgta
     8101 cgggcgggcg gggccgtctt ctccaggtgc accacgagcg ggagcacgta ctgcggggct
     8161 tcgtcccggt cggtcggacc gttgtggaag gggctcgggg agacagtggt ccgggaggag
     8221 gtgttctggg aggtgtcgtc gctgctcacg gtcccagtct gccaggcgga gattcgccgg
     8281 ctccttggct tgacgggccg ccccgggtga ggatgctcca catgaagagc gaccttttct
     8341 ccagcgagca catggcccag ccggccaccg cccccggcat gaccctgcag aacccgaagt
     8401 cgatcaagta cgccgtcaac ggcgagatgc acgcccgcca ggggtcgatg atcgccttcc
     8461 gcggcaatct ccagttcgaa cgcaagggcc agggcatcgg cggcctgctc aagcgcgcgg
     8521 tcaccggcga gggcctcgcg ctcatggccg tacgcgggca gggcgaggcg tggttcgcgc
     8581 acgaggccgc caactgcttc atcctggaga tggaccaggg cgaccagatc accatcaacg
     8641 gccgtaacgt cctctgtttc gacgccaccc tctcgtacga gatcaagacg gtgaagggcg
     8701 ccgggatggt cggcggcggc ctgttcaaca gcgtcttctc cggctacggc aagctcggcc
     8761 tgatgtgtga gggcacgccc atcgtgatcc cggtgacggg cgcccagccg gtgtacgtcg
     8821 acacggacgc ggtcgtcggc tggagccagc agctcacgac gtccctgcac cgctcccaga
     8881 gcgtcggatc gatggtgcgc ggcggctcgg gcgaggccgt gcagctgatg ctccagggcg
     8941 aggggttcgt catcgtgcgg ccgagcgagg ccaggccgga gcggaccgcc aactgacagt
     9001 ccggttctgc cgacccgatc cgacggcccg gtccggcgac cccgttacgt acgcttgtgg
     9061 gcatggacgc cgccgcgtac tgcgaaacgc ccgcacccgt gaccacggag gcggacgcgg
     9121 gcccgccgtt cgcggagtgc gtgctctgcc gcgagccgac cgagtacccg gagtcggcca
     9181 agggcgccac gctctgcccg gtctgcgcct ggcaggaggc ggggcgctcg gcctgctccg
     9241 gctgagtccg gtccggcctc cccctcggag gactccgcca gggcccgttc cgttcggcgt
     9301 ggacgggggg atcgggggac gggagggcgc cgcagcgcac cgtcgccctt catgccgagg
     9361 catctccggc cagggcacca ccggatggcg ccgactcccg gcaaccccgg cacggcgccg
     9421 ctccgcacgc gtgcgacacg cgatgccggt gatcggggga gaggcgcggg ggatgccgac
     9481 catccacgtt gccgtgtttc cgaccgtcgt cgttctggtg gaccgcgacg ccggccctcc
     9541 gggccggtgg cggtcggccc cgccgggaac tcctgcgtgc ggtgccgcct ccgcgcagtc
     9601 cgggtccgct gtcctgaccc ggggacgggt agcgggcact ccggccggcc cctgtccgac
     9661 ggctgtggag ccgccacaac cgacccgtca gccctccatc agctccgaca ccttcacgaa
     9721 ccggtaaccc cgcttccgca gctccggaac gattctgctg atcgcctccg ccgtgaccgg
     9781 cgccgcgctg cgcgtgcagt gcagtaccac cagcgacccc ggccgcaccc cgtccagcac
     9841 ctgttcggcc accgcccccg cgtccgtcgc gaacgcgtcg ccgctcacca cgtcccactg
     9901 caccgccgtc acgttcgccg gggccagcgc gcccagcgcc gcgtcgtcgt agcagccgcc
     9961 ggggaaacgg aagtacggca ccacgttccg tacaccggcc tcgcggaagg cggagaaggc
    10021 ccgctccacg tcgcgggcca tcgactcctt cgcgaccacc ggcaggccgt agcagtcggc
    10081 ggtgaaggcg tggtggctgt acgagtggtt ggcgacctcg aacaggtcgt ccgttccgat
    10141 gcccttcgcc tgcgccgggt actcctccgc ccaccgcccg gtcatgaaca cggtcgaggg
    10201 caccttcagc cgccgcagca gggcgatcag ctccgggttg tcgaagcgct cgcccgccgc
    10261 cgcccggggg ccctgatcgg ccgtcatgtc ggcgtcgaac gtgagcgcga cgaccttctc
    10321 cgccgcggcc cctcccgccg tacccccgcc tccggccgcc gcagccccgc ccgccggccg
    10381 ccgctcgaag accggggtgc gccccgccgg gccgggggcc atggtgggtg gcttcttcgg
    10441 ggcgggagag gtggtggcgg cggagggggc gggggtcgtg ggggaggacg gagcggggga
    10501 ggagggggcg agggcggtgg gtgcgggggt ggtgtccccg cacccggcca gcacaccgcc
    10561 gagcagcacc gccgcgaccg tcagggcagt tgtcttccac ttccgtgcag tagtgatcac
    10621 tcacggaaaa tatccgacat cccgcttata tggttatagc ggcactccgg tgcggcgtca
    10681 gtggtgcggt gccgacggtg cggctctcag cgccacggcc ccgtcaccgc gaacgtcgtc
    10741 cccggcgtgt agcagttcac gtacatcgtc gcgccgtccg gcgagaaggt gacccccgcg
    10801 aactcgcccc actccggctc ctcgggcgtc ccgatgttct gccgcccgcg cgccatcgcg
    10861 tacacctcgc cccgccgcgt cacccccagc acgtgctgcg cgccgccgcc gtcctcgcag
    10921 accatcagcc cgccgtcggc cgccaggcag atgttgtccg gggactcgcc ggggagctgg
    10981 atgtcggtgt ccgggccgaa gaccacgacc agggtcagcc ggcgcttctt cggctcgtac
    11041 cgccagatct gcccgaagtg gtcggccgcc gagccctcgt cgctgcgggc gaagctggag
    11101 acgaagtaga cgcacgagcc gccccagtag cagccctcca gcttctgggc gtgcgtgatg
    11161 cccttggggc cgaagtcctg gagccggatc ggggtctcgg ccgccagcgg gtccggtacg
    11221 ggtacccact ccacccggtc gaagctcgcc ccggtctcct ggaccacgga gaggtccggc
    11281 agccccggca cccgcatcgc ctccagcgcc ccgcccgccc gcagcg
//
