LOCUS       NZ_CP032543            10870 bp    DNA     linear   CON 15-NOV-2023
DEFINITION  Streptomyces griseus subsp. griseus strain ATCC 13273 chromosome,
            complete genome.
ACCESSION   NZ_CP032543
VERSION     NZ_CP032543.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces griseus subsp. griseus
  ORGANISM  Streptomyces griseus subsp. griseus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7238194)
  AUTHORS   Liu,J., Yu,B., Liu,X. and Shen,C.
  TITLE     Complete genome sequence of Streptomyces griseus ATCC 13273
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7238194)
  AUTHORS   Liu,J., Yu,B., Liu,X. and Shen,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2018) Jiangsu Key Laboratory of Tcm Evaluation and
            Translational Research, China Pharmaceutical University, Longmian
            Avenue, Nanjing, Jiangsu 211198, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2016
            Assembly Method                   :: SMRT analysis v. 2.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 88.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/15/2023 05:04:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,408
            CDSs (total)                      :: 6,322
            Genes (coding)                    :: 5,933
            CDSs (with protein)               :: 5,933
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 389
            CDSs (without protein)            :: 389
            Pseudo Genes (ambiguous residues) :: 0 of 389
            Pseudo Genes (frameshifted)       :: 299 of 389
            Pseudo Genes (incomplete)         :: 144 of 389
            Pseudo Genes (internal stop)      :: 16 of 389
            Pseudo Genes (multiple problems)  :: 65 of 389
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:22:13
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 125631
            Orig. end                         :: 136501
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032543.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from China pharmaceutical
            University, Jiangsu Key Laboratory of TCM Evaluation and
            Translational Research.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10870
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10870
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10870
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5870](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5870)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(569..585)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 18.11"
                     /tool="antismash"
     gene            630..995
                     /locus_tag="D6270_RS00620"
     CDS             630..995
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606636.1"
                     /locus_tag="D6270_RS00620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_109167742.1"
                     /transl_table=11
                     /translation="MSTTERGTTSGRQLAHPSVEEIRLETVLHALADPVRLRIARELAD
                     GHADMACIAFDLPVSKSTTTHHFKVLREAGVIRQHYEGTSRMSRLREADLEARFPGLLA
                     AVLEAARRSARSPAPSA"
     gene            complement(1069..2109)
                     /locus_tag="D6270_RS00625"
     CDS             complement(1069..2109)
                     /EC_number="1.1.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (19-269). E-value: 1.1e-12.
                     Score: 39.7. Matches aSDomain:
                     nrpspksdomains_D6270_RS00625_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019761872.1"
                     /locus_tag="D6270_RS00625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="WP_109167741.1"
                     /sec_met_domain="ADH_N (E-value: 1.5e-27, bitscore: 94.1,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 2.8e-21, bitscore:
                     74.4, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVAAYAAPRAKAPLVRTTIERRPVGEFDVLIDIKFAGICHSDI
                     HQARDGWGEGIFPMVPGHEIAGIVTETGPGVTKFKAGDRVGVGCMVDSCGKCDACLMGR
                     EQHCAEGNTQTYNALDRGGEPTYGGYSTHIVVTEGFVVGIPEGIALDEAAPLLCAGITT
                     YSPLRRWGAGPGKKVAVIGLGGLGHMAVKIAHTLGAEVTVLSQSLRKKDDGLKLGADHY
                     YATSDPKTFEELAGTFDIILSTVSAPLDFGAYLALLRTDGTLVNVGAPEEPVSLNLFSL
                     IGGNKSMAGSAIGGIAETQEMLDFCAVHGLGAEIEVIGAGQVNEAYERVLASDVRYRFV
                     IDTATI"
     aSDomain        complement(1303..2052)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_D6270_RS00625_PKS_ER.1"
                     /evalue="1.10E-12"
                     /label="D6270_RS00625_PKS_ER.1"
                     /locus_tag="D6270_RS00625"
                     /protein_end="269"
                     /protein_start="19"
                     /score="39.7"
                     /tool="antismash"
                     /translation="TIERRPVGEFDVLIDIKFAGICHSDIHQARDGWGEGIFPMVPGHE
                     IAGIVTETGPGVTKFKAGDRVGVGCMVDSCGKCDACLMGREQHCAEGNTQTYNALDRGG
                     EPTYGGYSTHIVVTEGFVVGIPEGIALDEAAPLLCAGITTYSPLRRWGAGPGKKVAVIG
                     LGGLGHMAVKIAHTLGAEVTVLSQSLRKKDDGLKLGADHYYATSDPKTFEELAGTFDII
                     LSTVSAPLDFGAYLALLRTDGTLVNVGA"
     CDS_motif       complement(1891..1926)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_D6270_RS00625_0001"
                     /evalue="1.10E-02"
                     /label="PKSI-ER_m2"
                     /locus_tag="D6270_RS00625"
                     /protein_end="73"
                     /protein_start="61"
                     /score="8.9"
                     /tool="antismash"
                     /translation="GHEIAGIVTETG"
     misc_feature    2172..2187
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 17.13"
                     /tool="antismash"
     gene            2263..3177
                     /locus_tag="D6270_RS00630"
     CDS             2263..3177
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012382294.1"
                     /locus_tag="D6270_RS00630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_109167740.1"
                     /transl_table=11
                     /translation="MDVSPDTSAPEPLDRRAELSEFLRTRRARLRPQDVGLPEFGRARR
                     VPGLRREELAQLAGVSVAYYTRLEQGNGRNVSAEVLDAIARALRLSDAEHAHLTHMAKP
                     AQHKKKRRPARVQRVRTGLLHLLDSLEGVPAYVSGARSDILAWNPMAAALFGDWGALPP
                     EQRNWARLVFLSPSYRDLFVEWDSKASDMVSYLRLYAGCHPDDPQLSALVGELSVKNDE
                     FRRLWATHNVKEKGHGTKLLRHPLVGDMALSYETLHLPDDQEQCLVTYHAEPGSESAQA
                     LRLLAGWGADAFGDAPRGLAGER"
     gene            complement(3202..3882)
                     /locus_tag="D6270_RS00635"
     CDS             complement(3202..3882)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018515159.1"
                     /locus_tag="D6270_RS00635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_109167739.1"
                     /transl_table=11
                     /translation="MTKQVRAARTRRALIRSAAIVFEQHGYGQARLALISSGANVSTGA
                     LHFHFENKAAVAQAVVAEASHGLLEVSTGIRRGTDTALQTLVDTSHALVELLGRDPVTR
                     AGFRLGYDGERCDEPGLHAQWRRCVGELLDDAAAAGTLTEGLSRPDVTAATVAATTGFE
                     VLGRHDSTWLSARTLTGFWRLLLPRLATPETLPLLDPSGTAAARQAVAAGRTGRERSGA
                     GAPG"
     gene            complement(4061..4921)
                     /locus_tag="D6270_RS00640"
     CDS             complement(4061..4921)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606640.1"
                     /locus_tag="D6270_RS00640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)H-binding protein"
                     /protein_id="WP_109167738.1"
                     /transl_table=11
                     /translation="MILVTGATGAVGREVAGQLAAAGPVRILARRPERVTVRGPGVDVV
                     AGEYADRASLDRALRGIDAVFLVTNSPTEPDDARVAAAAAAAGVRHLVKLSMMAVEEPD
                     ADDFITRLQRGNERTIRQSGVPWTFIRPRAFMSNTLSWATGIRSDGVVRDLYGDAPVAC
                     VDPRDVAAVAVAALTGAGHEGQAYAVSGPEVITAREQTAQLSGLLGRPLRFEELPLDRA
                     REALLAKYPQPVAEAFLESAERRRAGAKAAVLPTVEKLTGRPARTFRTWAADHTASFAA
                     TVRAG"
     gene            complement(5001..5870)
                     /locus_tag="D6270_RS00645"
     CDS             complement(5001..5870)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018515157.1"
                     /locus_tag="D6270_RS00645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_109167737.1"
                     /sec_met_domain="AfsA (E-value: 4.8e-25, bitscore: 86.0,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELVHRSRAEDAFPRTWEQVGPDRFHVEAVLPHDHPFFAPVGADR
                     HDPLLVTEAMRQAAMLAFHAGYGVPVGYHFLMADLDYATRPEGLVVGGLPTVIDVEVTC
                     SELKWRGRLPVQGRVDWVVHRAGQLVATGMGATRFTSPRVYRRMRGGSAEPCVAIPRTA
                     PVDAARAGRDRAQDVVLAESVREGVWELRVDTRHPTLFQRPNDHVPGMLLLEAARQAAC
                     LAAGGAGFVPAEGRTHFHRYAEFGSPCAIAAEVLPGAAPGEARVRVMGHQDGEPVFDTV
                     LSGPLGAS"
     misc_feature    complement(5955..5970)
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 23.65"
                     /tool="antismash"
     gene            6108..6746
                     /locus_tag="D6270_RS00650"
     CDS             6108..6746
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606642.1"
                     /locus_tag="D6270_RS00650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_109167736.1"
                     /transl_table=11
                     /translation="MAKQDRAIRTRQAILMAAAEVFEKYGYQAATITEILKIAGVTKGA
                     LYFHFPSKEELALGVIEAQEPPAPTPDQLLKLQKLVDMGMLFSHRLHTSLLTRAGVRLS
                     MDQQAQGLDRTGPFLRWQRTTHDLLAQAKQNGELLPHVNPTETADVYVAAFTGIQAVSQ
                     TLTNYRDLEQRYITLQRHILPSIAAPSILTALDLSPQRTTDLARLAPAY"
     gene            6823..7470
                     /locus_tag="D6270_RS00655"
     CDS             6823..7470
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018510005.1"
                     /locus_tag="D6270_RS00655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_109167735.1"
                     /transl_table=11
                     /translation="MQLPKDPSNVTSALVAAFPTRLAGDVKSVLAAMPEAGLARAMPFE
                     VEVQGETVAIPSRIYIEEPSADQEEPRLAGTQQVILHCLYSRHHDGRVRQRHLEQMVAS
                     DEPWVVPFVVQLAGEYVLEIIEAIGRGLPGLAVPGSAQRRLYGEFIARNPAFFARTERR
                     VVSYWSCHYRWKYGAFGTYPGCVLLEAFRAAVVEQVGGRWPRHAPHPAGGPG"
     gene            complement(7797..8408)
                     /locus_tag="D6270_RS00660"
     CDS             complement(7797..8408)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459226.1"
                     /locus_tag="D6270_RS00660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="WP_109167734.1"
                     /transl_table=11
                     /translation="MTNALTTAAAGFGTGLSLIVAIGAQNALVLRQGVRREAVLAVVVI
                     CALSDAVLITLGVAGVGAVVVAWPSALTVVSLLGGVFLIGYGVLAARRAFRPSALHAQD
                     APSGARRGAVLACLAMTWLNPHVYLDTVFLLGSVAADRGPLRWTFGLGAVLASVCWFAA
                     LGFGARLLSGVLARPSSWRVLDGLVAAMMIGLGTRLALTA"
     misc_feature    8428..8446
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 18.93"
                     /tool="antismash"
     misc_feature    8442..8457
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.09"
                     /tool="antismash"
     misc_feature    8445..8458
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.41"
                     /tool="antismash"
     misc_feature    complement(8445..8460)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.66"
                     /tool="antismash"
     gene            8475..9389
                     /locus_tag="D6270_RS00665"
     CDS             8475..9389
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1031: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606644.1"
                     /locus_tag="D6270_RS00665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="WP_109167733.1"
                     /transl_table=11
                     /translation="MMSGLPLDQVRTLLAVVDEGTFDAAADVLHVTPSAVSQRVKALEQ
                     RTGRVLLMRTKPVRPTESGEVVVRFARQLARLEQDAQSALGMSGPGEPTRLTVAVNSDS
                     LATWFLPALGRVPEELGVCYELRREDQDHTAALLREGLVMAAVTSSPEAVTGCSVRSLG
                     RMRYLPVATPEFAGRWLGRGTGTPLRELIADAPVVDFDRRDDLQDSFVRAVGAGRRAGF
                     RRHFVPTSEGFVDAVAAGMGWGMLPDVQARPLLGSGRLVLLAPDRHVDVPLYWQQWKLD
                     SPALATVAEAVVVEAAEALDGAP"
     gene            9474..10136
                     /locus_tag="D6270_RS00670"
     CDS             9474..10136
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060650.1"
                     /locus_tag="D6270_RS00670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /protein_id="WP_109167732.1"
                     /transl_table=11
                     /translation="MRVLLVEDDDDLRAVIAAGLREAGFAVDGTDDWPEADVMLHVTAY
                     DCVVLDRMLPSGDSLARLESRRRAGWSVPVLCLTALDALDDRLRGLESGADDYLAKPFA
                     MRELVLRVRSLTRRAAERLPAFLSCADVVMDLARREVRRGGVLLSLSPKEYAVLQQLLV
                     HRDSVVTRTALLEHCWDEMADPVSNVVDAVIAGIRRKLGAPGLVHTVRGQGFLMSSA"
ORIGIN
        1 taccagctgc tggatctcgt cctcggtcag ctcgtgcggg acgggcagcc cgtcgaacgc
       61 caccgggctg ggggccacgg accgccagcc gccgtcgctc tcggggacgt actcaccgcc
      121 gagccagggc cgatcggtgg acgccttgcg gccggcgtgg gcgagctgga tcgcggggac
      181 cgatccgtgg gaggcgatgg ccgcggcgat ccgggcgaag gcctcctgct ggcggtcgtt
      241 ccacagaccc aggtcccagg ggctgatccg cccgtcgggg cgtacgccgg tcgcctccgc
      301 catcacgagc ccggcgcctc cggcggcgcg gctcgcgagg tgggtgaggt ggaagtcggt
      361 cgggaccccg gcctcgggcc cgtgggaggc ggcggagtac atgcacatcg gggacatcca
      421 cacgcggttg gctatctcca gggaccgcag ggtcaggggc gtgaacagag cactcaccgg
      481 tatgtctcct ccgggtcggg gcctgggcgc cctgccggac gggaagggca gcagcgccat
      541 ggccaatacg atagacctcg tagtacggca gttgtcaaac tacgaggatg ttcgtacttt
      601 gggggtgcga gaaccgaagg aggccaccca tgtccacgac cgagcgcggc accacgtccg
      661 gccggcagct ggcccatccg tccgtggagg agatccgtct ggagaccgtc ctgcacgcgc
      721 tggccgaccc tgtccggctg cgtatcgcgc gtgagctggc ggacgggcac gcggacatgg
      781 cctgcatcgc gttcgacctt ccggtgagca agtcgaccac cacccaccac ttcaaggtgc
      841 tgcgcgaggc gggtgtcatc cgtcagcact acgaggggac ctcccgcatg agcaggctgc
      901 gcgaggccga cctggaggca cgcttcccgg gactgctggc ggccgtcctg gaggcggcac
      961 ggcgctccgc ccgctccccc gcgccttcgg cctgaacaac gcctgtacgt cgtccgcccc
     1021 gggcctcttc gcaggcccgg ggcggacgac gtacagctgt cggaccgctc agatggtcgc
     1081 ggtgtcgatc acgaagcggt agcggacgtc gctggcgagg acccgctcgt acgcctcgtt
     1141 cacctggccg gccccgatga cctcgatctc ggcgccgagg ccgtgcacgg cgcagaagtc
     1201 cagcatctcc tgggtctcgg cgatgccgcc gatcgcggag cccgccatcg acttgttgcc
     1261 gccgatgagc gagaagaggt tgagcgagac cggctcctcg ggcgcgccca cgttgaccag
     1321 ggtgccgtcc gtccgcagca gggcgaggta ggcgccgaag tccagcgggg ccgagaccgt
     1381 ggagaggatg atgtcgaagg ttccggccag ttcctcgaag gtcttggggt cgctggtcgc
     1441 gtagtagtgg tcggcgccca gcttgagccc gtcgtccttc ttgcgcagcg actgggagag
     1501 gaccgtgacc tcggcgccca gggtgtgcgc gatcttgacg gccatgtggc cgagcccgcc
     1561 gaggccgatg acggcgacct tcttcccggg gccggcgccc cagcggcgga gcggggagta
     1621 cgtggtgatg cccgcgcaga gcagcggcgc ggcctcgtcc agcgcgatgc cctcggggat
     1681 accgacgacg aacccctcgg tcacgacgat gtgcgtcgag tagccgccgt aggtgggctc
     1741 gccgccgcgg tcgagcgcgt tgtacgtctg ggtgttcccc tcggcgcagt gctgctcgcg
     1801 gcccatcaga caggcatcgc acttcccgca ggagtcgacc atgcagccga cgccgacacg
     1861 gtcgccggcc ttgaacttgg tgacgccggg gccggtctcg gtgacgatcc cggcgatctc
     1921 gtggccgggc accatcggga agatgccctc gccccagccg tcgcgggcct ggtggatgtc
     1981 ggagtggcag ataccggcga acttgatgtc gatcaggacg tcgaactcgc ccaccgggcg
     2041 gcgctcgatc gtggtgcgca ccagcggggc cttggcacgg ggagcggcgt aggcggcaac
     2101 ggtggtcatg ccggaggttc tcctctgggt gatgcggtag gaacgtctcc cagcatcagg
     2161 gtgcggccag ggtttaccca ggtcaccgct ttgcctacgt ccagtggtcc taccactggc
     2221 ggggacaggc tgccacgcgt acgaccagga ataatgggag ccatggacgt ttcgcccgac
     2281 acctccgccc ccgagcccct cgaccggcgc gccgagctca gcgagttcct gcgcacccgg
     2341 cgggcccggc tccggccgca ggacgtgggg ctgccggagt tcggccgggc ccgacgggtg
     2401 cccgggctgc gacgggagga gctggcgcag ctggccgggg tctcggtggc gtactacacg
     2461 cgcctggagc agggcaacgg gcgcaatgtg tcggccgagg tgctggacgc gatcgcccgg
     2521 gcgctgcggc tcagcgacgc cgagcacgcg catctcacgc atatggccaa gccggcgcag
     2581 cacaagaaga agcgtcggcc ggccagggtg cagcgggtgc ggacggggct gctgcacctc
     2641 ctggacagcc tggagggcgt ccccgcgtac gtctccgggg cccgctccga catcctcgcc
     2701 tggaacccga tggcggcggc gttgttcggc gactggggtg ccttgccgcc cgagcagcgc
     2761 aactgggcgc ggctggtgtt cctctccccc tcctaccggg acctgttcgt ggagtgggac
     2821 tccaaggcct cggacatggt cagctatctg cgcctgtacg cgggctgcca cccggacgat
     2881 ccgcagctct ccgcgctggt gggtgaactg tcggtcaaga acgacgagtt caggcggctg
     2941 tgggccacgc acaacgtgaa ggagaagggc cacggcacga agctgctccg gcatccgctc
     3001 gtcggcgaca tggccctgtc gtacgagacc ctgcatctgc cggacgacca ggaacagtgc
     3061 ctggtcacct accacgcgga accggggtcg gagtcggccc aggcactgcg cctgctggcc
     3121 ggctggggtg cggacgcgtt cggcgacgct ccccggggcc tggccgggga gcgctgaggg
     3181 cggaccgcag gggtccgggc gtcagcccgg ggcacccgcc ccggatctct cgcggccggt
     3241 cctgcccgcc gcgacggcct gcctcgccgc ggcggttccc gacgggtcca ggaggggcag
     3301 ggtctccgga gtggcgagac ggggcagcag caggcgccag aagccggtga gcgtccgggc
     3361 ggacagccag gtggagtcgt ggcggccgag cacctcgaag ccggtggtcg cggccaccgt
     3421 cgcggctgtg acgtcagggc gggacagccc ctcggtgagc gtgcccgcgg cggccgcatc
     3481 gtccagcaac tcgcctacgc agcgccgcca ctgcgcgtgc agacccggct cgtcgcatct
     3541 ctccccgtcg taaccgagcc ggaatccggc cctggtcacg gggtcgcggc cgaggagttc
     3601 caccagggcg tgcgaggtgt cgaccagggt ctgcagggcg gtgtccgttc cgcggcggat
     3661 gccggtggag acctccagca gcccgtggga ggcctccgcg accacggcct gggcgaccgc
     3721 ggccttgttc tcgaagtgga agtgcagcgc gcccgtgctg acattggcgc cggagctgat
     3781 cagcgccagg cgcgcctgtc cgtacccgtg ctgctcgaag acgatcgccg cggagcggat
     3841 cagcgcccgg cgggtgcgcg cggcgcgcac ctgcttggtc accgccgcct ccgtcggtgg
     3901 ggggagcggg cgggaagggg gcctgggccg tgagccacga gggccgggag ctctccaccg
     3961 gagccgtaca gcagtgtcat ggggaatatc aaaccaactt accggtttga tttccagggg
     4021 aacgtgtgcg cggacgggct ccatgaccgc gcgcggtccg ctatccggcg cggaccgtgg
     4081 cggcgaagga ggcggtgtgg tcggctgccc aggtacggaa cgtcctggcg gggcggcccg
     4141 tgagcttctc tacggtgggc agcacggcgg ccttggcccc cgcgcgccgg cgctccgcgc
     4201 tttcgaggaa cgcctcggcc accggttgag gatacttcgc gagcagcgcc tcgcgcgccc
     4261 ggtccagggg cagttcctcg aagcgcagcg gccggcccag cagtccggag agctgagccg
     4321 tctgctcccg cgcggtgatc acctccgggc ccgacacggc gtaggcctgc ccctcgtgac
     4381 ccgcaccggt gagggctgcc acggccaccg ccgcgacatc gcgcggatcg acgcacgcga
     4441 ccggtgcgtc cccgtagagg tcgcgcacca cgccgtccga ccggatgccc gtggcccagg
     4501 agagcgtgtt ggacatgaac gcccgggggc ggatgaacgt ccagggcacc ccggattgcc
     4561 tgatggtccg ttcgttgccc cgctggagcc gggtgatgaa gtcgtcggcg tccggctcct
     4621 ccaccgccat catcgacagc ttcacgaggt ggcgcacccc cgccgccgcg gccgccgcgg
     4681 cgaccctcgc gtcgtcgggc tcggtggggc tgttcgtcac gaggaacacc gcgtcgatcc
     4741 cgcgcagcgc ccggtcgaga gaggcgcggt ccgcgtactc gcccgccacg acgtcgactc
     4801 cgggcccgcg taccgtcacg cgctccggcc gcctcgccag gatccgtacc ggaccggcgg
     4861 ccgccagctg accggcgacc tcgcgcccca ccgccccggt cgctcctgtc accagaatca
     4921 cgccgctcac tcctcacaca ccgtcatcac cctgccggac acccgcgaca cagctcgcgc
     4981 tcccgcgcgc ggtcgcgccc tcagcttgcg cccagagggc ccgagagcac cgtgtcgaag
     5041 accggctccc cgtcctgatg gcccatcacc cggactctcg cctcgcccgg cgccgcgccc
     5101 ggcaggacct cggcggcgat cgcgcagggg ctgccgaact ccgcgtaccg gtggaagtgc
     5161 gtacgcccct cggcggggac gaacccggcg cccccggcgg ccaggcacgc ggcctgccga
     5221 gccgcctcca gcaacagcat gcccggcaca tggtcgttgg gccgctggaa gagcgtcgga
     5281 tgacgggtgt ccacccgcag ctcccagacc ccttcgcgca cgctctccgc gagcaccacg
     5341 tcctgcgccc ggtcacggcc cgcccgggcg gcgtcgaccg gcgccgtccg gggtatcgcc
     5401 acgcacggct ccgccgatcc accgcgcatc cgccggtaga cccgggggct ggtgaaccgg
     5461 gtcgcgccca tgccggtcgc gaccaactgg cccgcgcggt gcaccaccca gtcgacccgg
     5521 ccctgcaccg gcagcctgcc gcgccacttg agctcggagc aggtcacctc cacgtcgatc
     5581 acggtgggca gaccgcccac gacgaggcct tcggggcggg tcgcgtagtc gagatccgcc
     5641 atcaggaagt ggtagccgac ggggacgccg tacccggcgt ggaaggccag catcgccgcc
     5701 tgccgcatcg cctccgtcac cagcagcggg tcgtggcggt ccgcgcccac gggggcgaag
     5761 aacggatggt cgtgcggcag caccgcctcc acgtggaagc ggtccgggcc cacctgttcc
     5821 caagtgcgcg ggaacgcgtc ctccgccctg ctcctgtgaa ccagttccat tcccaccgga
     5881 tgcaccactc gggcctccga gcccacggcc gaggccgcta caggatcggt caacactgct
     5941 gcttcgggca taactccccc ccaggggtca tgtgatgggc gagccgttcc tgcggtgcgc
     6001 acgcgctgat aagatacaga ctaagcggtt ttttttctat gcccacagcc ctccgcagaa
     6061 cgcccgtccg gccgcacgac cggcacaccc cgacgaagga gacagggatg gccaagcagg
     6121 accgggcgat ccgcacccgc caggcaatcc tgatggcagc ggctgaggtt ttcgagaagt
     6181 acggctacca agcagccacc atcacggaaa tactcaagat cgccggggtc acgaaaggcg
     6241 cgctctactt ccatttcccc tccaaggaag agctcgcgct cggtgtgatc gaggcgcagg
     6301 agcctccggc gcccaccccc gaccaactcc tcaagctcca gaagctcgtg gacatgggga
     6361 tgctgttcag ccaccgcctc cacaccagtc tcctcacccg tgccggcgtc cgtctctcca
     6421 tggaccagca ggcccaaggc ctggaccgca ccggcccgtt cctgcgctgg cagcggacga
     6481 cccacgatct gctggcccaa gccaaacaga acggcgaact cctcccgcac gtcaacccca
     6541 ccgagaccgc ggatgtctat gtcgcggcct tcaccggcat ccaggccgtc tcccagaccc
     6601 tcacgaacta ccgtgatctg gagcagcgtt acatcacgct ccagcggcac atcctcccca
     6661 gcatcgccgc cccctccatc ctcaccgcgc tggacctcag cccccagcgc accaccgacc
     6721 tcgcccgcct cgcccccgcg tactgagccc cctaagagct gtcccgcagc gccgaggcgg
     6781 gcggtcgaga gacggagagg ttcgccgctt gtcctctctg ttgtgcagct gcccaaggat
     6841 ccgtcgaacg tcacctctgc cctggtggca gcgtttccga cccgtcttgc cggtgacgtg
     6901 aagagcgtcc tggcggccat gccggaggcc gggctcgcgc gggcgatgcc cttcgaggtc
     6961 gaggtgcagg gcgagaccgt ggccatcccg tcgcgcatct acatcgagga gccgagcgcc
     7021 gaccaggagg agccgcgcct ggccgggact cagcaggtga tcctgcactg cctgtactcc
     7081 cggcaccacg atggccgggt ccgccagcgg caccttgagc agatggtggc gtcggacgag
     7141 ccgtgggtgg tcccgttcgt ggtgcagttg gccggcgaat acgtgctgga gatcatcgag
     7201 gcgatcggcc gggggcttcc cggactcgcc gtcccgggct cggcccagcg ccgcctctac
     7261 ggggagttca tcgcccggaa tccggccttc ttcgcacgca cggagcggcg cgtggtgagc
     7321 tattggtcgt gccactaccg ctggaagtac ggagcgttcg ggacgtatcc cgggtgcgtc
     7381 cttctggaag cgttccgggc cgctgtggtg gaacaggtgg gcgggcggtg gccgcggcac
     7441 gcaccgcacc ccgccggcgg acccgggtga gggacctgcc cgaggtcggg tcgatgcggt
     7501 cgatgatccg gccggccgcc ggcttcgagt tcgcgcacag cggggccagt tggcacatgg
     7561 tcacgtcggt gcgccagcag gccgcgacca ggagaacgcg gtcttcaggt gaccggcctc
     7621 gcggaggacc cgtccgtggt ccgactgcgc tcccgcgctg caccgcggtc accaattcgc
     7681 ctgaacggcg cgggctcggc ccggtgagcg gacgggtcca ggacggctcc gatgccacgg
     7741 tcacaccagc cacaccaaga tcatctcacc cgtgaacagc agttgcggga cagccctcag
     7801 gcggtcaggg ccagccgggt gccgaggccg atcatcatcg cggcgacgag gccgtccagg
     7861 acgcgccacg acgacgggcg ggcgagtacc ccgctcaaca gccgggcgcc gaagccgagg
     7921 gcggcgaacc agcagacgct ggcgaggacc gcacccagcc cgaacgtcca gcgcagcggg
     7981 ccgcggtcgg cggcgacgga gccgagcagg aacaccgtgt cgaggtagac gtgcgggttg
     8041 agccaggtca tcgcgaggca ggcgaggacc gcaccccgcc tcgccccgga gggcgcgtcc
     8101 tgcgcatgga gggcggaggg gcggaaggcc cggcgtgcgg ccaggacgcc gtaaccgatc
     8161 aggaacacgc cgcccagcag ggagaccacg gtcagggcgg acggccaggc cacgacgacg
     8221 gcaccgaccc cggcgacgcc gagggtgatg aggaccgcgt cggacagggc gcagatgacg
     8281 accaccgcga ggacggcctc ccggcgcacg ccctgccgca ggaccagggc gttctgggcg
     8341 ccgatggcga cgatgaggga gaggccggta ccgaacccgg cggccgcggt ggtcagagcg
     8401 ttggtcatgg tccagacgct agggaggtcc tcgacacatg tacagctaat gattcttacg
     8461 tatcattagc tgctgtgatg tcggggcttc ctcttgatca ggtgcggacc ctgctggccg
     8521 tcgtcgacga aggcaccttc gacgcggcgg ccgacgtcct ccatgtgacg ccgtctgcgg
     8581 tcagccagcg ggtgaaggcc ctggagcagc gcacggggcg ggtgctcctg atgcggacga
     8641 agccggtgcg gcccaccgag tccggcgagg tcgtggtccg cttcgcccgc cagctggccc
     8701 ggctggagca ggacgcgcag tcggccctcg ggatgtcggg ccccggcgag ccgacgcggc
     8761 tgacggtcgc ggtgaactcc gattcgctgg cgacctggtt cctgcccgcg ctcggccgcg
     8821 taccggagga gctgggtgtc tgttacgagc tgcgccggga ggaccaggac catacggcgg
     8881 cgctgctccg ggaggggctg gtgatggcgg cggtgacctc gtcgcccgag gcggtgaccg
     8941 ggtgttcggt gcggagtctg ggccggatgc gctatctgcc ggtggcgacc ccggagttcg
     9001 ccgggcgctg gctcgggcgg gggacgggga ccccgttgcg cgagctgatc gcggacgcgc
     9061 cggtggtgga cttcgaccgg cgcgacgatc tccaggactc cttcgtccgc gcggtcgggg
     9121 ccggtcgccg ggcgggcttt cggcgccact tcgtgcccac gtcggaaggg ttcgtggacg
     9181 ccgtggcggc ggggatgggc tggggcatgc tccccgacgt ccaggcccgc ccgctgctgg
     9241 gctcggggcg gctggtcctc ctcgccccgg accgccacgt cgacgtgccc ctctactggc
     9301 agcagtggaa gctggactca ccggccctcg cgaccgtggc cgaggcggtg gtggtggagg
     9361 ccgccgaggc actcgacggg gccccttagg atggcgggtc tcccccgggt cagggctccc
     9421 gctacaggcc ggccgcgccc gggggccatc ccgagagacc cggaggcgtc cccttgcgcg
     9481 tgctgctggt ggaggacgac gacgacctgc gggcggtgat cgccgccggt ctgcgcgagg
     9541 ccgggttcgc cgtcgacggt acggacgact ggccggaggc ggacgtgatg ctgcacgtca
     9601 ccgcgtacga ctgtgtggtc ctggaccgga tgctgccctc cggtgactcc ctcgcgcggc
     9661 tggagagcag gcgccgggcg ggctggtcgg ttcccgtgct ctgcctcacc gccctggacg
     9721 ccctcgacga ccggctacgc gggctggaga gcggggcgga cgactacctc gccaagccct
     9781 tcgcgatgcg cgaactcgtc ctgcgggtac gcagtctgac ccggcgggcg gccgagcggc
     9841 tgcccgcctt cctgagctgc gcggatgtgg tgatggatct ggcgcggcgc gaggtgcgcc
     9901 ggggcggggt gctgctctcg ctgagcccga aggagtacgc cgtgctccag cagctcctgg
     9961 tccaccggga cagtgtcgtc acccgtacgg cgctgctgga gcactgctgg gacgagatgg
    10021 ccgacccggt ctccaatgtg gtggacgccg tcatcgccgg catacggcgc aagctcggcg
    10081 ccccgggcct cgtgcacacc gtgcgcggcc aggggttcct gatgagctcg gcatgaaccg
    10141 gcgcgaaggc ctcggctccg cgcggcgcgg ctccccggcc cggagtcggc tgcgccggct
    10201 ccagctgcgc ctgaccgtcg cgtacacgct gatcaccgtc gtcgggctga gctgtctgtc
    10261 ctggctggtg atccggaccg acgaccggtc ccgggaagcc tccgagtacg acgagatgcg
    10321 ccgccgggcc tccgtcgccg cctcgctcgt ctactacgag gacgaccgcg tccgcctcga
    10381 cggcctggcc gacgacgagg cgaccgcggg cacgccccag gtcctggtgc tggaggaccg
    10441 gcccggtggc gttccggccg tggtgttccg gggcggcgcc cagcagttcg acgtaccggc
    10501 cgcgacgctc gcgcgggcgg cgcgatcggc gatgcgcggc gaggagacgg tacgggagga
    10561 ggcgcacgac cgcacgggcg ggcccgtccg gctgctggcc gtgcccttct accacgacgc
    10621 gaccgacgag gtggcgggcg cggccgtcgc ggtcggggac ccggcgtacg ggtcggccga
    10681 gcaccgcagc ctggtcctct ccctcgtcgt cgggtgcgcc gcgctcaccg cgctggccgg
    10741 gctcaccggc catgtgctct ccgggcggtc gatgcggccc gcctggcagg cgctggagca
    10801 gcaggagcgg ctgctggccg acgcggcgca cgagctgcgg accccggtgg cggtgatgcg
    10861 cggttcggtg
//
