LOCUS       NZ_QZFU01000034         4043 bp    DNA     linear   CON 08-AUG-2024
DEFINITION  Nocardia panacis strain YIM PH 21724 NODE_567, whole genome shotgun
            sequence.
ACCESSION   NZ_QZFU01000034
VERSION     NZ_QZFU01000034.1
KEYWORDS    WGS; RefSeq.
SOURCE      Nocardia panacis
  ORGANISM  Nocardia panacis
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Nocardiaceae; Nocardia.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Miao,C.
  TITLE     YIM PH21274 draft genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Miao,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-SEP-2018) Yunnan University, Yunnan Institute of
            Microbiology, 121, Kunming 650091, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 29.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003598715.1-RS_2024_08_08
            Annotation Date                   :: 08/08/2024 03:06:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,880
            CDSs (total)                      :: 6,804
            Genes (coding)                    :: 6,707
            CDSs (with protein)               :: 6,707
            Genes (RNA)                       :: 76
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 32 of 97
            Pseudo Genes (incomplete)         :: 82 of 97
            Pseudo Genes (internal stop)      :: 7 of 97
            Pseudo Genes (multiple problems)  :: 22 of 97
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:26:29
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 4043
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            QZFU01000034.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4043
                     /collection_date="2015-05-12"
                     /db_xref="taxon:2340916"
                     /geo_loc_name="China"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Nocardia panacis"
                     /strain="YIM PH 21724"
                     /submitter_seqid="NODE_567"
                     /type_material="type material of Nocardia panacis"
     region          1..4043
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..4043
                     /SMILES="C([*])C(-O)"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..4043
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[5:3806](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      6..3806
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     gene            6..3806
                     /locus_tag="D5S18_RS25220"
                     /old_locus_tag="D5S18_25210"
     CDS             6..3806
                     /NRPS_PKS="Domain: PKS_AT (195-494). E-value: 7.5e-108.
                     Score: 352.3. Matches aSDomain:
                     nrpspksdomains_D5S18_RS25220_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_KR (816-995). E-value: 8.9e-58.
                     Score: 187.2. Matches aSDomain:
                     nrpspksdomains_D5S18_RS25220_PKS_KR.1"
                     /NRPS_PKS="Domain: ACP (1112-1184). E-value: 1.6e-29.
                     Score: 94.0. Matches aSDomain:
                     nrpspksdomains_D5S18_RS25220_ACP.1"
                     /NRPS_PKS="Domain: PKS_Docking_Cterm (1195-1260). E-value:
                     3.4e-05. Score: 16.6. Matches aSDomain:
                     nrpspksdomains_D5S18_RS25220_PKS_Docking_Cterm.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /locus_tag="D5S18_RS25220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="D5S18_25210"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_282434881.1"
                     /sec_met_domain="mod_KS (E-value: 1.3e-43, bitscore: 136.4,
                     seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 2.5e-109, bitscore:
                     352.3, seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 9.2e-60, bitscore:
                     188.7, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 5.1e-16, bitscore:
                     46.2, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGHTQAAAGVAGVIKMVMAMRHGVLPPTLHADEPTPHVDWSAGRV
                     ELLTEARDWPETGRLRRAGVSAFGVSGTNAHVILEQAPAVEPTLVADVQAPVAEFAETA
                     DSALVWVLSGRGGEALAGQARRLGRYLERCPDLGVADVARSLLGRSVFEHRAVIVGGDR
                     SELMAGLAAVAEQVPASGVVSGVAHEQGKTVLVFPGQGAQWLGMGRQLLASAPVFAEKM
                     AECDKAFASLVEWSLLDVLAGEDSVWLERVDVVQPVLFAVMVSLAELWRSFGVEPDAVV
                     GHSQGEIAAAYVSGALSLEDAARIVILRSAALTVLEGQGGMMSVGLPLKQVEQLLEGFD
                     GLSVAAANGPRATVVSGLSEQLDALLAVCEAREVRARRVPVDYASHSAQVERLREPLLE
                     ALAGLQARSSQVVFYSTVTGAVLDTSELDARYWYANLREPVRFEHTVQGLYRDGYTVFI
                     EASPHPLLVVDIEQIGDALEEGKSSAAVVVGSLRRGEDGVARFAQSVAQLGVSGVGVEW
                     ESVLRGRGRWVELPTYAFQRRRYWLEKTADRTSSNAIDAEFWRAVEKGDLGALGIDPDR
                     SVDEALPLLSSWRRRHQDQTAIGSWSYRIDWRLLPDEPVGVRGNWLVVGSSGVDAGEGV
                     LEALRESGVHTRYLEINADRMSRLDVVDLLRDATGRSELSGVLSFAALDDRQCSQSSSV
                     TQGLLANLLLLQALGDLGGGQRLWCVTSGAVNVVSEERISSTMQVQMWGLGQVAGLEYP
                     QWWGGLIDLPDGWDQTVVQRLLASLSRSDGEDQLAVRRSGVYGRRMVRASLPGSAAADG
                     WKPTGTVLITGGTGGIGRNLAHWVADNGAEHVVLASRRGRRAPDVEVLETQLRALGSRV
                     TIATCDMTRRDDVAALLSAIDDDPAPLTAVIHAAGVGSLTPLTEIDLSSLITVVEPKIA
                     GAWYLDELLGDRPLDAFVMFSSGAVTWGSAGSAAYAAANAYLDGLAHDRRTRGLTATAL
                     AWGGWAGGGMVEADGSAELLSHGSSIADFLALNGVRLMDPDLALQALSQAVGRGETTMT
                     IADIEWRRFAPIYAASRDRCLLHELSDAQAALQAEQAEAEAEALAGLRERAAGLSESDR
                     YAAVLEMVCGQVAAVLGYSGAEAIDVDRNFRDLGFDSLTAVEARNRLNTITGLRLPATL
                     VFDYPTPNAVTGYILQELFDTTESVEDSFLEDLERFESTLRRIDPDIAGSDSVTRRIEG
                     IIEKLRELKTPDNIGNADDSSLIDSLDPESLVRHIIEKQMD"
     CDS_motif       9..68
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_D5S18_RS25220_0001"
                     /evalue="7.20E-01"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="D5S18_RS25220"
                     /protein_end="21"
                     /protein_start="1"
                     /score="3.1"
                     /tool="antismash"
                     /translation="GHTQAAAGVAGVIKMVMAMR"
     CDS_motif       537..560
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_D5S18_RS25220_0002"
                     /evalue="7.50E+01"
                     /label="PKSI-AT-mM_m5"
                     /locus_tag="D5S18_RS25220"
                     /protein_end="185"
                     /protein_start="177"
                     /score="-2.0"
                     /tool="antismash"
                     /translation="PASGVVSG"
     CDS_motif       585..641
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_D5S18_RS25220_0003"
                     /evalue="8.30E-09"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="D5S18_RS25220"
                     /protein_end="212"
                     /protein_start="193"
                     /score="27.2"
                     /tool="antismash"
                     /translation="VLVFPGQGAQWLGMGRQLL"
     aSDomain        591..1487
                     /ASF="Methylmalonyl-CoA specific"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_D5S18_RS25220_PKS_AT.1"
                     /evalue="7.50E-108"
                     /label="D5S18_RS25220_PKS_AT.1"
                     /locus_tag="D5S18_RS25220"
                     /protein_end="494"
                     /protein_start="195"
                     /score="352.3"
                     /specificity="substrate consensus: pk"
                     /specificity="PKS signature: Methylmalonyl-CoA"
                     /specificity="Minowa: emal"
                     /tool="antismash"
                     /translation="VFPGQGAQWLGMGRQLLASAPVFAEKMAECDKAFASLVEWSLLDV
                     LAGEDSVWLERVDVVQPVLFAVMVSLAELWRSFGVEPDAVVGHSQGEIAAAYVSGALSL
                     EDAARIVILRSAALTVLEGQGGMMSVGLPLKQVEQLLEGFDGLSVAAANGPRATVVSGL
                     SEQLDALLAVCEAREVRARRVPVDYASHSAQVERLREPLLEALAGLQARSSQVVFYSTV
                     TGAVLDTSELDARYWYANLREPVRFEHTVQGLYRDGYTVFIEASPHPLLVVDIEQIGDA
                     LEEGKSSAAVVVGSLRRG"
     aSModule        591..3557
                     /complete
                     /domains="nrpspksdomains_D5S18_RS25220_PKS_AT.1"
                     /domains="nrpspksdomains_D5S18_RS25220_PKS_KR.1"
                     /domains="nrpspksdomains_D5S18_RS25220_ACP.1"
                     /locus_tags="D5S18_RS25220"
                     /monomer_pairings="pk -> pk"
                     /starter_module
                     /tool="antismash"
                     /type="pks"
     CDS_motif       750..821
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_D5S18_RS25220_0004"
                     /evalue="6.30E-12"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="D5S18_RS25220"
                     /protein_end="272"
                     /protein_start="248"
                     /score="37.2"
                     /tool="antismash"
                     /translation="LERVDVVQPVLFAVMVSLAELWRS"
     CDS_motif       825..935
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_D5S18_RS25220_0005"
                     /evalue="1.20E-19"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="D5S18_RS25220"
                     /protein_end="310"
                     /protein_start="273"
                     /score="62.0"
                     /tool="antismash"
                     /translation="GVEPDAVVGHSQGEIAAAYVSGALSLEDAARIVILRS"
     CDS_motif       1038..1076
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_D5S18_RS25220_0006"
                     /evalue="1.90E-03"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="D5S18_RS25220"
                     /protein_end="357"
                     /protein_start="344"
                     /score="11.6"
                     /tool="antismash"
                     /translation="AAANGPRATVVSG"
     CDS_motif       1146..1178
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_D5S18_RS25220_0007"
                     /evalue="3.10E-04"
                     /label="PKSI-AT-mM_m6"
                     /locus_tag="D5S18_RS25220"
                     /protein_end="391"
                     /protein_start="380"
                     /score="13.4"
                     /tool="antismash"
                     /translation="VDYASHSAQVE"
     CDS_motif       1284..1331
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_D5S18_RS25220_0008"
                     /evalue="6.00E-06"
                     /label="PKSI-AT-mM_m8"
                     /locus_tag="D5S18_RS25220"
                     /protein_end="442"
                     /protein_start="426"
                     /score="18.1"
                     /tool="antismash"
                     /translation="LDARYWYANLREPVRF"
     aSDomain        2454..2990
                     /ASF="KR domain putatively catalyzing L-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_D5S18_RS25220_PKS_KR.1"
                     /evalue="8.90E-58"
                     /label="D5S18_RS25220_PKS_KR.1"
                     /locus_tag="D5S18_RS25220"
                     /protein_end="995"
                     /protein_start="816"
                     /score="187.2"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: A1"
                     /tool="antismash"
                     /translation="GTVLITGGTGGIGRNLAHWVADNGAEHVVLASRRGRRAPDVEVLE
                     TQLRALGSRVTIATCDMTRRDDVAALLSAIDDDPAPLTAVIHAAGVGSLTPLTEIDLSS
                     LITVVEPKIAGAWYLDELLGDRPLDAFVMFSSGAVTWGSAGSAAYAAANAYLDGLAHDR
                     RTRGLTATALAWGGWA"
     CDS_motif       2457..2555
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_D5S18_RS25220_0009"
                     /evalue="2.20E-14"
                     /label="PKSI-KR_m1"
                     /locus_tag="D5S18_RS25220"
                     /protein_end="850"
                     /protein_start="817"
                     /score="45.9"
                     /tool="antismash"
                     /translation="TVLITGGTGGIGRNLAHWVADNGAEHVVLASRR"
     CDS_motif       2835..2945
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_D5S18_RS25220_0010"
                     /evalue="1.30E-16"
                     /label="PKSI-KR_m4"
                     /locus_tag="D5S18_RS25220"
                     /protein_end="980"
                     /protein_start="943"
                     /score="53.1"
                     /tool="antismash"
                     /translation="LDAFVMFSSGAVTWGSAGSAAYAAANAYLDGLAHDRR"
     aSDomain        3342..3557
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_D5S18_RS25220_ACP.1"
                     /evalue="1.60E-29"
                     /label="D5S18_RS25220_ACP.1"
                     /locus_tag="D5S18_RS25220"
                     /protein_end="1184"
                     /protein_start="1112"
                     /score="94.0"
                     /tool="antismash"
                     /translation="EMVCGQVAAVLGYSGAEAIDVDRNFRDLGFDSLTAVEARNRLNTI
                     TGLRLPATLVFDYPTPNAVTGYILQEL"
     aSDomain        3591..3785
                     /aSDomain="PKS_Docking_Cterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_D5S18_RS25220_PKS_Docking_Cterm.
                     1"
                     /evalue="3.40E-05"
                     /label="D5S18_RS25220_PKS_Docking_Cterm.1"
                     /locus_tag="D5S18_RS25220"
                     /protein_end="1260"
                     /protein_start="1195"
                     /score="16.6"
                     /tool="antismash"
                     /translation="LEDLERFESTLRRIDPDIAGSDSVTRRIEGIIEKLRELKTPDNIG
                     NADDSSLIDSLDPESLVRHI"
     gene            3929..>4043
                     /locus_tag="D5S18_RS36460"
     CDS             3929..>4043
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /locus_tag="D5S18_RS36460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="WP_158607953.1"
                     /sec_met_domain="ketoacyl-synt (E-value: 6.6e-13, bitscore:
                     35.9, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAIVGIGCRFPGGVSSAGELWQLLAEGRDVIGGFPTDR"
ORIGIN
        1 ccaatatcgg tcacactcag gcggctgcgg gggtggctgg tgtgatcaag atggtgatgg
       61 cgatgcggca tggggtgctg ccgccgacgt tgcatgcgga tgagccgact ccgcatgtgg
      121 attggtctgc gggtcgggtg gagttgctga ctgaggcgcg ggactggccc gagacgggtc
      181 gtctgcggcg cgcgggtgtg tccgccttcg gtgtcagtgg taccaacgct cacgtgatcc
      241 ttgagcaggc accggctgtc gagccgacac tggttgccga tgtgcaggcg ccggtagccg
      301 aattcgccga gacggccgac tctgcgttgg tgtgggtgtt gtcggggcgt ggtggggagg
      361 cgttggctgg gcaggcgcgg cggttggggc ggtatttgga gcggtgtccg gatctcggcg
      421 tggccgatgt ggcgcggtcg ttgttggggc ggtcggtgtt cgagcatcgt gcggtgatcg
      481 tgggtggtga tcggtcggag ttgatggccg ggttggctgc ggtggctgag caggttccgg
      541 cgtcgggtgt ggtgtcgggt gtggcgcatg agcagggtaa gacggtgttg gtgttcccgg
      601 ggcagggtgc gcagtggctg ggaatgggtc ggcaactgct ggcatcggca ccggtgttcg
      661 ccgagaagat ggccgagtgc gacaaggcgt tcgcttcgtt ggtggagtgg tcgctgctgg
      721 atgtgcttgc cggcgaggat tcggtgtggc tcgagcgggt cgatgtggtc cagccggtgt
      781 tattcgcggt gatggtatcg ctggctgagc tgtggcgttc tttcggtgtc gaacccgacg
      841 cggtggtcgg tcattcccag ggtgaaatcg ctgcggcgta tgtgtcgggg gcgttgtccc
      901 ttgaggatgc ggcccggatc gtgatcctgc gttcggcggc tttgaccgtg ctggagggtc
      961 agggcggcat gatgtcggtc gggttgccgc tgaagcaggt cgagcagctg ctcgagggtt
     1021 tcgacgggtt gtcggtggct gcggccaacg ggccccgggc cacggtggtc tcgggcctga
     1081 gcgagcagct ggatgccctg ctggcggtgt gcgaagcccg cgaggtccgc gccagacggg
     1141 tcccggtaga ttacgcctcg cactcggcgc aggtcgagcg gttgcgtgaa ccgttgctgg
     1201 aggcgttggc cggactccag gcccgctcgt cgcaggtggt gttctattcg acggtgaccg
     1261 gcgcagtgct cgacaccagc gaattggatg cgcggtactg gtatgccaat ctgcgcgaac
     1321 ctgttcgttt cgagcacact gtccaagggt tgtatcgcga tgggtacacg gtgttcatcg
     1381 aggccagccc gcatccgttg ctggtggtcg atatcgagca gatcggtgac gcgctagaag
     1441 aggggaagtc atctgcggcg gtggtggtgg gctcgttgcg tcgtggcgag gacggcgtgg
     1501 cgcggtttgc tcaatcggtc gcgcagttgg gcgtgtcggg tgtaggtgtg gagtgggaat
     1561 ccgtattgcg ggggcgtggc cggtgggtcg agttgcctac gtatgcgttc cagcggcgcc
     1621 ggtactggct cgaaaagacc gccgatcgga cctcctccaa cgcgatcgat gccgagttct
     1681 ggcgtgcggt cgaaaaggga gatctgggtg cgttggggat agatccggat cgatctgtcg
     1741 atgaggcgct accgctgctg tcatcgtggc ggcgacgcca tcaggatcag acagcaatcg
     1801 gttcatggtc ctatcggatc gattggcgac tactgccgga cgagccggtg ggtgtgcgcg
     1861 gaaattggct tgtagtcggg tccagtggcg tggatgcggg tgaaggcgtg ctcgaagcgt
     1921 tgcgggaatc cggtgtccat acccgctacc ttgagatcaa cgccgaccgt atgtcgcgcc
     1981 tggatgtggt ggaccttctg cgcgatgcga ccgggcggag cgagttgagt ggtgtgttgt
     2041 cgtttgcggc gctcgatgat cggcagtgtt cgcagagttc gtccgtgacg caggggttgt
     2101 tggcgaatct gctgctgctg caggcactgg gcgatctcgg tggcggacag cgtttgtggt
     2161 gtgtgaccag cggtgcggtg aacgtcgttt ccgaggagcg gatcagcagc acgatgcagg
     2221 tccagatgtg gggtctgggt caggtagccg ggctggagta cccgcagtgg tggggtggcc
     2281 tgatcgatct gccggacgga tgggaccaga cggttgtgca gcgtctgttg gcttcgttgt
     2341 cccgaagcga tggggaagat cagttggcgg tccggcggtc aggggtctac ggccgccgga
     2401 tggtgcgggc atcgttgccc ggatccgccg ccgccgatgg gtggaagccg acaggaacgg
     2461 tcctgatcac cggcggtacc ggcggaatcg gcaggaatct ggcgcactgg gtggccgaca
     2521 acggcgccga gcatgtcgtg ctggccagtc gtcgtggtcg acgggcaccc gatgtcgagg
     2581 tgctcgaaac gcaactgcgt gcgctgggca gccgggtcac gatcgcgacc tgcgatatga
     2641 cccgacgcga tgacgtggcc gcgctgttgt cggcgatcga cgacgacccc gctccgctca
     2701 cagcggtcat ccatgccgcc ggtgtcggct cgttgacccc gcttaccgag atcgacctat
     2761 cgtcactgat cacggtggtg gaacccaaga tcgccggtgc gtggtatcta gacgaactgc
     2821 tcggtgaccg ccccctggat gcgttcgtca tgttctcctc cggcgcggtg acttggggca
     2881 gcgctggcag cgcggcctac gccgccgcga atgcctacct cgacggcctg gcccacgacc
     2941 gcagaacgcg gggcctcacc gctaccgcac tggcatgggg aggctgggct ggcgggggaa
     3001 tggtcgaggc cgacggcagc gccgaactcc tgtcccacgg aagctccatc gcagacttcc
     3061 tggcactcaa tggtgttcgg ttgatggatc cggatctggc cttgcaggca ttgagccaag
     3121 cggtgggtcg tggtgagacg accatgacga tcgccgatat cgagtggcgg cggttcgcac
     3181 cgatctacgc ggcgtcgagg gatcgttgtc tgctgcacga actgtccgac gcgcaggcgg
     3241 cgctgcaagc tgagcaagcc gaagccgaag ccgaagctct ggcaggcttg cgggagcggg
     3301 cggcgggatt gtccgagtcc gatcggtatg cggcagtgct cgagatggtc tgcggtcagg
     3361 tcgcggcggt attgggttat tccggtgctg aggcgatcga tgtcgaccgc aatttccggg
     3421 acctgggatt cgattcgctg accgcagtgg aagcccgaaa tcgtctcaac accatcaccg
     3481 ggctgcgact accggccacg ctggtcttcg actacccgac accgaacgcg gtgaccggat
     3541 acatcctcca agaactcttc gataccaccg aatcagtgga agacagcttc ttggaagatc
     3601 tggagaggtt tgaatcgacc ctccgccgga ttgatccgga tatcgcggga agtgattcgg
     3661 tcactcgtcg catcgaggga attatcgaga aacttcgcga gttgaagacg ccggacaata
     3721 tcggtaacgc tgatgattcc agcctgatcg actcgttgga tcccgaaagt ctcgtacgtc
     3781 atattattga gaagcagatg gactgaccga ggggatttgc gtgtccattt cgattgatga
     3841 cctgaccaga gctctgcgaa tttcggcgcg agataacgag tccctacgaa gggagaatcg
     3901 ggagctacgt gcactagcgt cggagccggt ggcaatcgtg gggatcggat gtcggttccc
     3961 gggtggggtg tcctctgcgg gggagttgtg gcagttgctg gccgaggggc gtgacgtgat
     4021 cggtgggttc ccgaccgatc ggg
//
