LOCUS       NZ_QZFU01000030         4657 bp    DNA     linear   CON 08-AUG-2024
DEFINITION  Nocardia panacis strain YIM PH 21724 NODE_511, whole genome shotgun
            sequence.
ACCESSION   NZ_QZFU01000030
VERSION     NZ_QZFU01000030.1
KEYWORDS    WGS; RefSeq.
SOURCE      Nocardia panacis
  ORGANISM  Nocardia panacis
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Nocardiaceae; Nocardia.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Miao,C.
  TITLE     YIM PH21274 draft genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Miao,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-SEP-2018) Yunnan University, Yunnan Institute of
            Microbiology, 121, Kunming 650091, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 29.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003598715.1-RS_2024_08_08
            Annotation Date                   :: 08/08/2024 03:06:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,880
            CDSs (total)                      :: 6,804
            Genes (coding)                    :: 6,707
            CDSs (with protein)               :: 6,707
            Genes (RNA)                       :: 76
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 4, 3 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 32 of 97
            Pseudo Genes (incomplete)         :: 82 of 97
            Pseudo Genes (internal stop)      :: 7 of 97
            Pseudo Genes (multiple problems)  :: 22 of 97
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:26:29
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 4657
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            QZFU01000030.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            complement(<1..229)
                     /locus_tag="D5S18_RS36440"
                     /old_locus_tag="D5S18_24795"
     CDS             complement(<1..229)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /locus_tag="D5S18_RS36440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="D5S18_24795"
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="WP_147404127.1"
                     /sec_met_domain="mod_KS (E-value: 2e-18, bitscore: 53.4,
                     seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 2.3e-12, bitscore:
                     34.1, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSTTNGLAEALRASLKDRERLHQEVARLREIAHEPIAIVGVGCR
                     FPGGVRSAGDLWSVVSRGRDVISGFPGDRGW"
     source          1..4657
                     /collection_date="2015-05-12"
                     /db_xref="taxon:2340916"
                     /geo_loc_name="China"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Nocardia panacis"
                     /strain="YIM PH 21724"
                     /submitter_seqid="NODE_511"
                     /type_material="type material of Nocardia panacis"
     region          1..4657
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..4657
                     /SMILES="C=CC(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..4657
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[365:4652](-)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(366..4652)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     gene            complement(366..4652)
                     /locus_tag="D5S18_RS24805"
                     /old_locus_tag="D5S18_24800"
     CDS             complement(366..4652)
                     /EC_number="2.3.1.-"
                     /EC_number="6.4.-.-"
                     /GO_component="GO:0034081 - polyketide synthase complex
                     [Evidence IEA]"
                     /GO_process="GO:0030639 - polyketide biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_AT (185-462). E-value: 5.7e-105.
                     Score: 342.9. Matches aSDomain:
                     nrpspksdomains_D5S18_RS24805_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_DH (531-697). E-value: 5.3e-39.
                     Score: 126.2. Matches aSDomain:
                     nrpspksdomains_D5S18_RS24805_PKS_DH.1"
                     /NRPS_PKS="Domain: PKS_KR (1022-1202). E-value: 1.1e-57.
                     Score: 186.9. Matches aSDomain:
                     nrpspksdomains_D5S18_RS24805_PKS_KR.1"
                     /NRPS_PKS="Domain: PKS_PP (1294-1375). E-value: 4.1e-25.
                     Score: 79.9. Matches aSDomain:
                     nrpspksdomains_D5S18_RS24805_PKS_PP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1021: malonyl CoA-acyl carrier protein transacylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /locus_tag="D5S18_RS24805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="D5S18_24800"
                     /product="type I polyketide synthase"
                     /protein_id="WP_282434875.1"
                     /sec_met_domain="mod_KS (E-value: 6.6e-42, bitscore: 130.8,
                     seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 1.9e-106, bitscore:
                     342.9, seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 2.7e-60, bitscore:
                     190.5, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 7.8e-10,
                     bitscore: 25.4, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.2e-16, bitscore:
                     48.3, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGHTQAAAGVAGVIKMVMAMRHGVLPPTLHADEPTPHVDWSSGGL
                     RLLTESTPWGEDGRTRRAAVSSFGISGTNAHVILEQAPAREPAAELPNETAPAVLPWVI
                     SAKTKAGLRSQAEQLLALAAADVSPGVVGCALATTRTQFEHRAAVVGSSLDDFRKGLVA
                     FRDGRLVANVVSGVAGVGKTAFMFTGQGAQRSGMGRELYDAFPVFADALDEVCGYLSAE
                     LGQRIQDVMWDEDGDLLHQTGYTQPALFALETALYRLVRHWGIEPNYLIGHSIGEITAA
                     HVAGVLSVQDAAVLVAARARLMQGLTARGAMAAVVAAEGDVVPYLNDEVSLAAVNGSLS
                     VVISGAEGSVLELAAVLEQRGHKTRRLTVSNAFHSACMDPILEDFREVAAGLKYGEPTI
                     PIISNVTGAVATIDELRSPDYWVEHLRRTVRFHDGVETLRGLGVTRFLELGPDATLSSF
                     VNQHELAAIPLLRKKQSELKTVVTALAKIQIRGVAVDWVGFYPANSGIDLPTYPFQHQT
                     YWLDGSLDTSSRTGHPLINASVELEDGGVIYQGQISTQRHSWLLDHIVHERVVVPGTTW
                     VELVSWAGRQVGCTEVAELTHHAPLVLPSGIFVQIQLHILGPDRADRREFSLRCRAERS
                     PGAPWVTLARGIVSVPDHLATVTAEIAVATWPPEGAQPLDSEDYYPSVARKGFYGWGLA
                     FHSLRKVWRRDDEIFAEIRYADSLDSGSFDLHPALFDATMHALGVDGAPETLSGLVADE
                     GTDYARPRIPFMWRGVAIRAQGRRAIRVRIAESSEEGVSITLFDDSGQVVGTIRGMVLL
                     AVSGEQLRSSAGIDQIDSLYEITWNTTPTASSYVDWPSGWMGVAAADLAFSGSNYESMS
                     ALTAAVRSGGDIPELVLMQCEHGVRDDAGTAASRAVVSAVAAIQEWLSEPMLTSCRLVL
                     VTRGMAVPLDESAGEPAQSAVWGLVRSAQAEHPGRIVLVDVDDEEDSLRALPGYLAEDT
                     PEPQFVVRRGQRLVPRLARVRATSDALSSFDPHGTVLITGGTGTLGAQVARHLAERYSV
                     PRLILVSRRGPSANGAAELASELKRLGADVEILACDVSDREALAAMLRNLTTRYRVTTV
                     VHAAGVIDDAVISELDASRIEMVFRPKASAAWHLHQLTMELELEKFVLFSAAAGTIGSV
                     GQANYAAANAFLDGLACHRRDIGLPAISLAWGLWDDRSGMTAHLTGVDRERISRQGITG
                     LSNAHGLALFDASLSYDRPCLVPAGIDAAKLGDVATLSPVFRSLTAKRLNSNASQEQPN
                     PDRSSAHELRVKTGRERERAVHDLLVANIAAVIGRPGESIDSDAPFREIGLDSLMAIEL
                     RHRLSTATELPLSATFAFDHPTVQAVSRYLCGLLDSTAPEGDIGIPERHDLQRGEIARP
                     VVQEIDEMDDEALIQLAHESPWSGDY"
     aSDomain        complement(528..770)
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_D5S18_RS24805_PKS_PP.1"
                     /evalue="4.10E-25"
                     /label="D5S18_RS24805_PKS_PP.1"
                     /locus_tag="D5S18_RS24805"
                     /protein_end="1375"
                     /protein_start="1294"
                     /score="79.9"
                     /tool="antismash"
                     /translation="VKTGRERERAVHDLLVANIAAVIGRPGESIDSDAPFREIGLDSLM
                     AIELRHRLSTATELPLSATFAFDHPTVQAVSRYLCG"
     aSModule        complement(528..4097)
                     /complete
                     /domains="nrpspksdomains_D5S18_RS24805_PKS_AT.1"
                     /domains="nrpspksdomains_D5S18_RS24805_PKS_DH.1"
                     /domains="nrpspksdomains_D5S18_RS24805_PKS_KR.1"
                     /domains="nrpspksdomains_D5S18_RS24805_PKS_PP.1"
                     /locus_tags="D5S18_RS24805"
                     /monomer_pairings="mal -> ccmal"
                     /starter_module
                     /tool="antismash"
                     /type="pks"
     aSDomain        complement(1047..1586)
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_D5S18_RS24805_PKS_KR.1"
                     /evalue="1.10E-57"
                     /label="D5S18_RS24805_PKS_KR.1"
                     /locus_tag="D5S18_RS24805"
                     /protein_end="1202"
                     /protein_start="1022"
                     /score="186.9"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: (unknown)"
                     /tool="antismash"
                     /translation="GTVLITGGTGTLGAQVARHLAERYSVPRLILVSRRGPSANGAAEL
                     ASELKRLGADVEILACDVSDREALAAMLRNLTTRYRVTTVVHAAGVIDDAVISELDASR
                     IEMVFRPKASAAWHLHQLTMELELEKFVLFSAAAGTIGSVGQANYAAANAFLDGLACHR
                     RDIGLPAISLAWGLWDD"
     CDS_motif       complement(1095..1196)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_D5S18_RS24805_0009"
                     /evalue="8.80E-18"
                     /label="PKSI-KR_m4"
                     /locus_tag="D5S18_RS24805"
                     /protein_end="1186"
                     /protein_start="1152"
                     /score="56.9"
                     /tool="antismash"
                     /translation="FVLFSAAAGTIGSVGQANYAAANAFLDGLACHRR"
     CDS_motif       complement(1482..1583)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_D5S18_RS24805_0008"
                     /evalue="4.70E-10"
                     /label="PKSI-KR_m1"
                     /locus_tag="D5S18_RS24805"
                     /protein_end="1057"
                     /protein_start="1023"
                     /score="32.1"
                     /tool="antismash"
                     /translation="TVLITGGTGTLGAQVARHLAERYSVPRLILVSRR"
     aSDomain        complement(2562..3059)
                     /ASF="catalytic triad H,G,P inconclusive"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_D5S18_RS24805_PKS_DH.1"
                     /evalue="5.30E-39"
                     /label="D5S18_RS24805_PKS_DH.1"
                     /locus_tag="D5S18_RS24805"
                     /protein_end="697"
                     /protein_start="531"
                     /score="126.2"
                     /tool="antismash"
                     /translation="HPLINASVELEDGGVIYQGQISTQRHSWLLDHIVHERVVVPGTTW
                     VELVSWAGRQVGCTEVAELTHHAPLVLPSGIFVQIQLHILGPDRADRREFSLRCRAERS
                     PGAPWVTLARGIVSVPDHLATVTAEIAVATWPPEGAQPLDSEDYYPSVARKGFYGWGLA
                     FHS"
     CDS_motif       complement(2916..2978)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_D5S18_RS24805_0007"
                     /evalue="1.10E-05"
                     /label="PKSI-DH_m2"
                     /locus_tag="D5S18_RS24805"
                     /protein_end="579"
                     /protein_start="558"
                     /score="18.0"
                     /tool="antismash"
                     /translation="WLLDHIVHERVVVPGTTWVEL"
     aSDomain        complement(3267..4097)
                     /ASF="Neither malonyl-CoA or methylmalonyl-CoA specific"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_D5S18_RS24805_PKS_AT.1"
                     /evalue="5.70E-105"
                     /label="D5S18_RS24805_PKS_AT.1"
                     /locus_tag="D5S18_RS24805"
                     /protein_end="462"
                     /protein_start="185"
                     /score="342.9"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="MFTGQGAQRSGMGRELYDAFPVFADALDEVCGYLSAELGQRIQDV
                     MWDEDGDLLHQTGYTQPALFALETALYRLVRHWGIEPNYLIGHSIGEITAAHVAGVLSV
                     QDAAVLVAARARLMQGLTARGAMAAVVAAEGDVVPYLNDEVSLAAVNGSLSVVISGAEG
                     SVLELAAVLEQRGHKTRRLTVSNAFHSACMDPILEDFREVAAGLKYGEPTIPIISNVTG
                     AVATIDELRSPDYWVEHLRRTVRFHDGVETLRGLGVTRFLELGPDATLSSFVNQH"
     CDS_motif       complement(3360..3395)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_D5S18_RS24805_0006"
                     /evalue="3.30E-04"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="D5S18_RS24805"
                     /protein_end="431"
                     /protein_start="419"
                     /score="14.1"
                     /tool="antismash"
                     /translation="YWVEHLRRTVRF"
     CDS_motif       complement(3432..3464)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_D5S18_RS24805_0005"
                     /evalue="5.30E-03"
                     /label="PKSI-AT-M_m7"
                     /locus_tag="D5S18_RS24805"
                     /protein_end="407"
                     /protein_start="396"
                     /score="9.6"
                     /tool="antismash"
                     /translation="PTIPIISNVTG"
     CDS_motif       complement(3618..3656)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_D5S18_RS24805_0004"
                     /evalue="5.60E-03"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="D5S18_RS24805"
                     /protein_end="345"
                     /protein_start="332"
                     /score="10.1"
                     /tool="antismash"
                     /translation="AAVNGSLSVVISG"
     CDS_motif       complement(3741..3863)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_D5S18_RS24805_0003"
                     /evalue="5.10E-23"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="D5S18_RS24805"
                     /protein_end="304"
                     /protein_start="263"
                     /score="73.4"
                     /tool="antismash"
                     /translation="GIEPNYLIGHSIGEITAAHVAGVLSVQDAAVLVAARARLMQ"
     CDS_motif       complement(4047..4094)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_D5S18_RS24805_0002"
                     /evalue="7.00E-06"
                     /label="PKSI-AT-M_m1"
                     /locus_tag="D5S18_RS24805"
                     /protein_end="202"
                     /protein_start="186"
                     /score="18.5"
                     /tool="antismash"
                     /translation="FTGQGAQRSGMGRELY"
     CDS_motif       complement(4590..4649)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_D5S18_RS24805_0001"
                     /evalue="8.20E-01"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="D5S18_RS24805"
                     /protein_end="21"
                     /protein_start="1"
                     /score="2.9"
                     /tool="antismash"
                     /translation="GHTQAAAGVAGVIKMVMAMR"
ORIGIN
        1 cccaaccacg atcaccagga aaccccgaga tcacatcccg cccccgcgac accacagacc
       61 acagatcacc cgcagaccgc accccacccg gaaaccgaca acccacaccc acaatcgcaa
      121 tcggctcgtg cgcgatctcg cgcaggcgcg ccacctcttg gtggagccgc tccctgtctt
      181 tgagcgaggc acggagcgct tctgcgaggc cattcgttgt cgaattcatc taagtccctt
      241 tgatcagtac agccatcgaa gccgaactcc aaacagctaa tcgctcattc gattgacgca
      301 ccagagccgc tcactccccc gactagcgag tcgaatggcc ctcgcagcgt ccttccggtc
      361 tgccctcagt agtcccccga ccacggggac tcgtgagcga gttgaattag cgcctcatca
      421 tccatctcat cgatttcctg aaccaccggt cgagctattt caccgcgctg gaggtcatgc
      481 cgttctggta tgccgatgtc gccctctggc gcggtcgagt cgaggaggcc gcacagatac
      541 cgcgataccg cttgtacagt cggatggtcg aatgcgaacg tagccgacaa gggaagttcg
      601 gtcgctgtgg acagtctgtg tctgagctcg atcgccatca gcgagtccaa tccgatttca
      661 cggaacggag catccgaatc tattgattcg cccggcctgc cgataaccgc agctatattc
      721 gcgacaagaa ggtcgtgcac agcccgttcc cgttcccgcc ctgtcttaac gcgcaattcg
      781 tgggcacttg agcgatcagg attcggttgt tcttgggaag cgttgctgtt cagacgcttc
      841 gccgtcaaac tccggaagac tggcgaaagt gtcgcaacat ctcccagctt cgccgcgtcg
      901 atccctgcgg gaaccaggca cgggcggtca taactcagcg atgcgtcgaa gagagctagt
      961 ccatgcgcgt tcgacaatcc ggtgatgcct tggcgggaaa tacgttcacg gtccaccccg
     1021 gtcagatgag cagtcatgcc gcttcggtcg tcccacagac cccaagccaa cgatatcgca
     1081 ggcagaccta tgtctcgccg gtggcaggcg agaccgtcca ggaatgcgtt cgctgcggca
     1141 tagttcgcct gacccacgct tccaatggtc cccgccgccg cggagaagag gacgaatttc
     1201 tccagttcga gctccatggt gagctgatgc aaatgccagg ctgccgaggc cttcggccga
     1261 aacaccattt cgatacgcga ggcgtccagc tcggagatga ccgcgtcgtc gatcactccg
     1321 gccgcgtgta cgaccgttgt aacacgatac cgggttgtca ggttgcgaag catggctgcc
     1381 agcgcttcac gatccgagac atcgcaggcc aggatctcga catccgcacc gagacgcttc
     1441 agctcggacg caagctcggc agcgccgttg gcgctcggtc cccgtcgact gaccaggatc
     1501 agccgtggaa cggaataccg ttccgcaagg tgacgggcca cctgcgcacc gagagtgcct
     1561 gtgccaccgg tgatcagcac tgttccgtga ggatcgaatg acgagagtgc gtctgatgtc
     1621 gctcgaaccc gcgccagtcg cggaacgagg cgttgacctc gcctgaccac aaactgaggc
     1681 tcgggcgtgt cttccgcaag gtatccgggg agcgcccgca gtgaatcttc ttcatcgtcc
     1741 acgtcgacca gcacaatccg gcctggatgc tcggcttgtg ccgaccgaac gagtccccaa
     1801 accgctgatt gcgcaggctc ccccgccgac tcgtccagtg gaacagccat accccgggtg
     1861 acgaggacca gccggcacga cgtcaacatc ggctctgaca accactcttg gatagcggcc
     1921 accgccgaca ctacggctcg gctcgcagcc gtaccggcat cgtcgcgaac tccgtgttcg
     1981 cactgcataa gcaccaactc cgggatatca cccccgcttc ggactgcggc cgtcaaagcc
     2041 gacatggact catagttgga cccagagaag gccagatccg ccgcagcgac gcccatccat
     2101 ccactcggcc aatcgacgta cgacgaagca gtcggagttg tgttccaggt gatttcgtac
     2161 aatgaatcga tctggtcgat gccagcagag ctacgcaatt gctcgccgga gacggcgagc
     2221 aggaccatcc cacggatagt tccgacgacc tggccggaat cgtcgaagag cgttatcgat
     2281 acgccttctt cactagactc ggctattcga acccgtattg cccgccgacc ctgcgctctg
     2341 atggccacac cccgccacat gaaaggaatg cgcggacgag cgtaatccgt gccttcatcg
     2401 gcaaccaggc cggatagggt ctctggtgcc ccgtcgacac cgagtgcatg catcgttgcg
     2461 tcgaacaggg ctggatgcag atcgaatgag ccggagtcga gagaatcggc atatctgatc
     2521 tccgcgaaga tctcgtcgtc gcgtcgccat accttccgca acgaatggaa ggcaagtccc
     2581 cagccgtaga atccttttcg agcgacgctt ggatagtaat cctcactatc gagtggctgt
     2641 gccccctccg gcggccacgt cgctacggcg atctctgcgg tcacagtagc gagatgatct
     2701 ggcacactga caattccgcg tgcgagggtg acccacggcg cccctggact gcgctctgcg
     2761 cggcaccgca acgagaactc tcgcctgtcc gctctgtccg gccccagtat atgcagctga
     2821 atctgtacga aaatcccgga cggcaacaca aggggtgcgt ggtgagtcag ctccgcgact
     2881 tcggtgcagc ccacctgcct tcctgcccaa gacaccaact ccacccaggt tgtgcccggc
     2941 acaacaactc tctcgtgcac aatatgatcg aggagccaag aatgccgttg cgtcgaaatc
     3001 tggccttgat aaatcacacc gccgtcctcc aactcgaccg atgcgtttat caacggatga
     3061 ccggtgcggg agctcgtatc cagcgagccg tccagccagt acgtttggtg ttgaaacgga
     3121 tatgtcggta ggtcgattcc actgttggca gggtaaaatc cgacccagtc cacagcaaca
     3181 ccgcgaattt gaattttggc gagtgcggtg accaccgttt tcagttcgga ctgcttcttc
     3241 cgcagcagcg gtatcgccgc gagttcgtgc tggttgacga acgaggagag cgtggcatcc
     3301 ggaccgagct caaggaagcg ggtgacgccg agaccgcgaa gcgtctcgac tccgtcgtgg
     3361 aagcgcaccg tccgtcgaag gtgttccacc cagtaatccg gcgaacgcaa ttcatcgatg
     3421 gtggcgacag ctcccgtaac attggaaatg atcggtatgg tcggctcgcc gtatttcagg
     3481 cctgccgcta cttcccgaaa atcttcgagg atcgggtcca tgcaggccga gtggaatgcg
     3541 ttgctgacgg tcagccgacg cgtcttgtga ccacgctgct ccaagacggc ggcgagttcg
     3601 agaacgcttc cttccgcacc ggaaatgaca acagagagac ttccattgac ggccgccaac
     3661 gatacctcgt cattcagata cgggacgaca tcgccctcgg ccgccacgac ggccgccatg
     3721 gcaccgcgtg cggtcaggcc ttgcatgagg cgtgcacgtg ccgcaacaag gacggcggcg
     3781 tcctgaacag atagcacgcc tgcgacgtgg gccgcagtga tctcaccgat cgaatgaccg
     3841 atgaggtagt ttggctcgat gccccagtgt cgtaccagcc ggtagagggc tgtctcaaga
     3901 gcgaatagcg caggttgggt gtagccggtc tgatgcagca ggtctccgtc ctcgtcccac
     3961 atgacatcct ggatacgctg gccgagctcg gcggacagat agccacacac ctcgtcaagc
     4021 gcgtcagcga agaccggaaa cgcgtcgtag agctcacgtc ccatgcccga gcgttgagct
     4081 ccctgtccgg taaacatgaa cgcggtcttc ccaacgcctg cgacaccgga aaccacgttc
     4141 gcgaccagcc ggccatcgcg gaaggcaacg agacccttcc ggaagtcgtc gagcgagctg
     4201 ccgaccactg ccgctcggtg ctcgaattgc gtgcgcgtag tcgccaacgc acacccgaca
     4261 actcctggac tgacgtccgc agccgccagt gcgagaagtt gttccgcctg agatctcaac
     4321 cccgcctttg tcttcgcgga gatcacccag ggcaggaccg ccggcgccgt ttcgttcggc
     4381 aactccgccg ctggctcgcg tgcgggtgcc tgttcgagaa tgacatgtgc gttggtaccg
     4441 ctgataccga acgaggacac cgctgctcgt cgggttcgcc cgtcctcgcc ccacggagtg
     4501 gactcggtca acaaccgcaa tccaccactc gaccaatcca catgcggagt cggctcatcc
     4561 gcatgcaacg tcggcggcag caccccatgc cgcatcgcca tcaccatctt gatcacacca
     4621 gccacccccg cagccgcctg agtgtgaccg atattgg
//
