LOCUS       NZ_KZ984531            10282 bp    DNA     linear   CON 31-MAR-2024
DEFINITION  Staphylococcus argenteus strain PR02 PR02_scaffold_1, whole genome
            shotgun sequence.
ACCESSION   NZ_KZ984531
VERSION     NZ_KZ984531.1
KEYWORDS    WGS; RefSeq.
SOURCE      Staphylococcus argenteus
  ORGANISM  Staphylococcus argenteus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2648450)
  AUTHORS   Lee,L.S., Teh,L.K., Zainuddin,Z.F. and Salleh,M.Z.
  TITLE     Tracing the evolution of antibiotic resistance in Staphylococcus
            aureus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2648450)
  AUTHORS   Lee,L.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2013) Faculty of Pharmacy, Universiti Teknologi
            MARA, Level 7, Block FF3, Bandar Puncak Alam, Selangor 42300,
            Malaysia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: CLC Genomics Workbench v. September 2012
            Expected Final Version            :: no
            Genome Coverage                   :: 500.0x
            Sequencing Technology             :: Illumina GAIIx
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003569945.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 03:29:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,610
            CDSs (total)                      :: 2,561
            Genes (coding)                    :: 2,485
            CDSs (with protein)               :: 2,485
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 33 of 76
            Pseudo Genes (incomplete)         :: 41 of 76
            Pseudo Genes (internal stop)      :: 18 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:46:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 352063
            Orig. end                         :: 362345
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KZ984531.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria available from Pharmacogenomics Lab, Universiti Teknologi
            MARA. Source DNA available from Pharmacogenomics Lab, Universiti
            Teknologi MARA.
FEATURES             Location/Qualifiers
     region          1..10282
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10282
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10282
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[<5000:5282](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      <5001..5282
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(459..474)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.78"
                     /tool="antismash"
     tRNA            complement(486..574)
                     /anticodon=(pos:complement(352600..352602),aa:Ser,seq:gct)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="AHP18_RS01745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /product="tRNA-Ser"
     gene            complement(486..574)
                     /locus_tag="AHP18_RS01745"
     misc_feature    complement(512..521)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 15.28"
                     /tool="antismash"
     tRNA            complement(577..651)
                     /anticodon=(pos:complement(352680..352682),aa:Asn,seq:gtt)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="AHP18_RS01750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /product="tRNA-Asn"
     gene            complement(577..651)
                     /locus_tag="AHP18_RS01750"
     assembly_gap    652..718
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     tRNA            complement(720..794)
                     /anticodon=(pos:complement(352823..352825),aa:Asn,seq:gtt)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="AHP18_RS01755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /product="tRNA-Asn"
     gene            complement(720..794)
                     /locus_tag="AHP18_RS01755"
     gene            complement(936..1505)
                     /locus_tag="AHP18_RS01760"
     CDS             complement(936..1505)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /locus_tag="AHP18_RS01760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="competence protein ComK"
                     /protein_id="WP_000287261.1"
                     /transl_table=11
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFKV
                     QKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQHENIWLN
                     MHYIENIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMVSKNP
                     DQPIDYNKATLNVFEALTRYSLFEDK"
     misc_feature    1572..1585
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.83"
                     /tool="antismash"
     misc_feature    complement(1575..1591)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 25.02"
                     /tool="antismash"
     gene            1715..1933
                     /locus_tag="AHP18_RS01765"
     CDS             1715..1933
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /locus_tag="AHP18_RS01765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="IDEAL domain-containing protein"
                     /protein_id="WP_072507491.1"
                     /transl_table=11
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRELVELID
                     DALVNKDEAAFNQYTTEYKNLEAFLGE"
     gene            complement(2014..3000)
                     /locus_tag="AHP18_RS01770"
     CDS             complement(2014..3000)
                     /EC_number="6.3.1.20"
                     /GO_function="GO:0017118 - lipoyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /locus_tag="AHP18_RS01770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lipoate--protein ligase"
                     /protein_id="WP_072507490.1"
                     /transl_table=11
                     /translation="MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIVG
                     KNQNTIEEVNQPYIDSHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKFTE
                     PIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQNAL
                     KVNPAKIKSKGIKSVRKRVANIQEFLNEPLEIEEFKKIILKTIFGETEVETYHLTDEDW
                     KNIEKLSNDKYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIFGDFFG
                     VGDVTDLENALVGCLHDFEHIEESLSEYDLYHYFGDIDKYELIRLMS"
     gene            3195..3371
                     /locus_tag="AHP18_RS01775"
     CDS             3195..3371
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /locus_tag="AHP18_RS01775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YkvS family protein"
                     /protein_id="WP_000214898.1"
                     /transl_table=11
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLPE
                     KTVINHKRYKIVE"
     gene            3386..3988
                     /locus_tag="AHP18_RS01780"
     CDS             3386..3988
                     /EC_number="3.4.-.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /locus_tag="AHP18_RS01780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="CPBP family glutamic-type intramembrane protease"
                     /protein_id="WP_072507489.1"
                     /sec_met_domain="Abi (E-value: 4.6e-07, bitscore: 27.4,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKISKALTWFIISFVIFHLILFIMWGEHQEYWYLYTGIMLIAGI
                     SYVFYQRDIESKRLLTSIGIGIITAIILIMLQLLFSLITSNLSYSSLIKELARTGVNWK
                     WQMLVTLLFVIPCHELYMRTVLQKELTHFSLPKWAVIIITAVCSSSLFIYLDNWWIVTF
                     IFVAQVILSLSYEYTRRIATTSVAQIVAIILLLIFNA"
     misc_feature    complement(4069..4087)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 25.94"
                     /tool="antismash"
     misc_feature    4932..4946
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 26.74"
                     /tool="antismash"
     gene            <5001..5282
                     /locus_tag="AHP18_RS01790"
                     /pseudo=""
     CDS             <5001..5282
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790905.1"
                     /locus_tag="AHP18_RS01790"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="lactococcin 972 family bacteriocin"
                     /pseudo=""
                     /sec_met_domain="Lactococcin_972 (E-value: 1.2e-22,
                     bitscore: 77.5, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="KNKSFDSLLYLSLALGLFRVAYASTEYAEGCAWTHGVGSKYVWSY
                     YYHGHKGHGATAIGKYRSFSGYTRAGVKAKASATKNNWWVNRAYYNIY"
     misc_feature    5002..5015
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 21.37"
                     /tool="antismash"
     gene            5326..7290
                     /locus_tag="AHP18_RS01795"
     CDS             5326..7290
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /locus_tag="AHP18_RS01795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_072507488.1"
                     /transl_table=11
                     /translation="MKWFKLILDVTIFILIAILLFVYTYKENEEILPDTKYPIVVTDWN
                     KKYSKNEIYKHINQFAKNENVAIYKSTSNYTNKKVDKDIYVFNKSKATPITPFNSKYNI
                     HYINDNELLKKDIKGSYFVKDTNFDVSKFINLLKQYGVSAESYKVNHMMIAVGVIKQMN
                     LVVPLSALLIVYFIYYIFEKNINFKGYAIKYLNGFTLGKIIFEKFSMKCTYWLTLMITQ
                     IILTTCILWILNYTGNLDLFILRLVLLSSLFILTISSINLWTFLMLLNLNIANMIKGKQ
                     HFKTIRFINTVCKSILLVLISTVIIENASVIKDLNKIKETEKYWNVLDDYYTVEFAPYH
                     ETKQSLIDNMVRSEQLVKASEAENNAILFKPKSDSADSDNFSPDEGNVILVNNQFWSIY
                     HKQFQPDIPIKNQKNNVEVIIPQKFHAMRNEINQAYHSWFEFVQNKNNKENKLSMQFIN
                     TNDYRIFTFDARDSRHLSFIDSPIIVNVQASDLSNDFYYAMISQGGYLFKNYDALVKNI
                     EKYHLDGEISGITNYKDSVMEMYHENNLKLTVFIFSQIIIAIILVIIILFDVKYYFEQH
                     RKLLVIKKLYGYSTLRANYQYLLINNIFVVFIGILTNVFINSHYLVMIFATILVVQILL
                     QFCSLYYLDRRFNEVIKEF"
     gene            7293..7613
                     /locus_tag="AHP18_RS01800"
     CDS             7293..7613
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /locus_tag="AHP18_RS01800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YxeA family protein"
                     /protein_id="WP_119024926.1"
                     /transl_table=11
                     /translation="MKFIIAILLGLIMAITIAFTIIHHPILDRFNPFLKTESSFAKVPK
                     GTQQYVNITAYSEKGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEREETPKEVR
                     QE"
     gene            7610..8251
                     /locus_tag="AHP18_RS01805"
     CDS             7610..8251
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /locus_tag="AHP18_RS01805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_072507487.1"
                     /transl_table=11
                     /translation="MIELKDLTIQKGNTSILKKLNLKFQCGKSYALIGKSGCGKSTLLN
                     VIAGLEKMGKQHIYFNGQEERFKPNFYRDKLGYLFQNYGLIDNMSVNENLEIGLAYKKI
                     SKKEKEQLKICYLEQFGLYDSLKRKVHTLSGGEQQRVALIRMILKDPTIILADEPTGAL
                     DPKTGQLIIQTLFNLVDENKVLILATHDMAIANRCDVVIDLEQFKNVVSM"
     gene            complement(8339..8587)
                     /locus_tag="AHP18_RS01810"
     CDS             complement(8339..8587)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499524.1"
                     /locus_tag="AHP18_RS01810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_240430928.1"
                     /transl_table=11
                     /translation="MLTRTYNIIGIISCLLSFIIMALPIIWYTASALWFFPGAIIILLL
                     SLFIVFCYIKTKNQLHLILLVLNIIILLFFSLPLLLS"
     gene            8923..9210
                     /locus_tag="AHP18_RS01815"
     CDS             8923..9210
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499525.1"
                     /locus_tag="AHP18_RS01815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_072507486.1"
                     /transl_table=11
                     /translation="MEKGIFNYDNAKVLKLDTNQLNENIKVIDNVFKNYEQLEPTIEIE
                     NGKSVLKLNGHFIASIIGPLNVNKLNNLYVDEDFYHTYNELIVKYTEVKE"
ORIGIN
        1 gatgaacaat taagtttaac aatcattgat gaaacgttat gtacaatgtg gcaggggttt
       61 aactatgcga ttgatatgag ccgagatccg gggcaccgtg ttgaattaga aaatattaat
      121 ttagaaaaga cttatagaat tacaatgaca gattattgtt ttagaaatta caaaaattat
      181 ttgaaagatg caatcgttca tgagtcttat gaggatacaa tgagtacgtt gattgcagaa
      241 aagttaaaag atccagatta tagcattaaa tgtaaagaga actttatagt tcgaaaatag
      301 tgaaccgttg catcataaca aaatagacaa gattaactaa acttaaaaac ctttcttaga
      361 gaatttcagc taagaaaggt ttttaagttt tagaatatgc aatatagctg attgacaagt
      421 atttaaaatc tactttaaaa actaaataaa aaagcttaaa tggtttatac catttaagca
      481 tgtaacggaa acggagggat tcgaaccctc gcgccgcttt cgcgacctac acccttagca
      541 ggggcgcctc ttcagccaac ttgagtacgt ttccaatggc tccacaggta ggactcgaac
      601 ctacgaccga tcggttaaca gccgatagct ctaccactga gctactgtgg annnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat
      721 ggctccacag gtaggactcg aacctacgac cgatcggtta acagccgata gctctaccac
      781 tgagctactg tggattaatt tgtatgtgtt tgaatatcaa gcacaaatat tattataaca
      841 agattaagtt aatttgcaat agataccata aaaaaatatg cagaacattt acactatatt
      901 tataaaacat tccgcatatt ttaaaaataa acaatttatt tatcttcgaa tagggaataa
      961 cgtgttaatg cttcaaatac gtttaatgtg gctttattat aatctatagg ttgatcggga
     1021 tttttagaaa ccatacgaga ttgtttatct accatatagt agtaaaaaat ggaattgtta
     1081 tattgatgcc ataaactgtg gtatgaaaca tgaagaataa ttgattcgtt attaataaaa
     1141 gttactttac atttacgatt ttttaattct ttaatatttt ctatataatg catatttagc
     1201 caaatgtttt cgtgttgtct gtcagaatga gttgggaaaa aataagttgg aaataagggt
     1261 gttagtaaaa taggtggttt actagaaatg ccagtaatgc ggtttgtttc tgctttcttg
     1321 ccaaggtatg tattaccata aaatttgcaa gagcgttcta tgattttctg gactttaaat
     1381 ggattttgta tacgcgtttt atcaaaccgg ataatttcac taccatttct ttgatcatca
     1441 tcatcaaatg ctggtcgaat aaccatgtct cctttgcgta tcacataaat gttttgagaa
     1501 tacataagca aaacctcgct ttattaagtt ttaaaccatt aatataaaaa tacaattaat
     1561 gatataaaga tattttataa taaaatttca agttgtcaag ttattgtttt ggtgaaaaac
     1621 tacaataatt tttgcttaat agtcagaata ttcattgatt tcaaaattag aaaaatgtat
     1681 acttattata aattaaagaa aggtagtgat ttctatgaaa tacaatacta atgttaaaca
     1741 tacaacttta gaagcatttg tcacaaccgt caatgattta ggtattgaat taattatcaa
     1801 tgaagcactc cgagaggtaa gaaaacgaga gctcgtagaa ctcatagatg atgcactcgt
     1861 caataaagat gaagcagcat ttaatcaata tacgacagaa tacaaaaatt tggaggcatt
     1921 tctcggtgaa taatattgat tcgaattcca taacgcaatc ataaaaggac tattcttaac
     1981 atttcataca tgtaagaata gtccttttat caattacgac attaatcgaa ttaattcgta
     2041 tttatcaata tcaccaaaat aatgatataa atcatattct gatagagatt cttctatatg
     2101 ttcaaagtcg tgtagacaac caactaatgc attttcaagg tcagttacat ctccaacacc
     2161 aaagaaatcc ccgaaaattt ttgcatgttc gattttacca cgcttaacat caaatttgat
     2221 ttgtacaaat cctttttcaa atttttcctc gcgttcaaag ttatatttag ggtttctacc
     2281 ataattccat tcccaagttc tatatttgtc attgcttaat ttttcaatat ttttccaatc
     2341 ttcatccgtt aaatgatacg tttctacttc agtttctcca aagattgttt tcaagataat
     2401 tttcttaaat tcttcaatct ctaatggttc atttaaaaat tcttgaatgt ttgctacgcg
     2461 tttacgtacc gacttaatac ctttagactt aattttagct ggattaactt ttagagcgtt
     2521 ttgaacttca tctaaatcac tgtttaacat taatgtacca tgactaaaca ttctattttt
     2581 aacttttacc attgcgttac ctgagatttt tgcttgtcct acttgaatat cattgcgccc
     2641 agttaattcg gcattaacac ctaaagattg tagcgcttga acaattggtt ctgtgaattt
     2701 ctggaagttg tggaaactgt taccatcatc atcagtaata aaactaaagt ttaaattgcc
     2761 tgtatcatga taaacagcgc caccacctga aattcttcta actacatcga tgttatgtga
     2821 atcaatatat ggctgattta cttcttcaat tgtattttga ttcttcccaa caatgataga
     2881 tggtctattt atgtaaaaca aaaagtaact ttcttcagct ggtagatttt ttaaaacata
     2941 ctcttccata gctaaattta aagttggatc tgtaatattg ttgttactaa taaatttcat
     3001 tacaatctct cccttatttc tatattctct tcttatttat gccctaagtt tgcttaaaat
     3061 gccaattatt tactcataaa tcattctaaa tattggctta aagttacatt ttattatttt
     3121 tgattgttaa tagtttattt gttaagtgaa ttttagtaca atataattta gttatgattt
     3181 aaggaggact taaaatgact gttgcagaag tgggtaacat tgttgagttt atggatggat
     3241 taagaggtcg tgttgaaaaa atcaacgata actctgttat tgttgactta acaattatgg
     3301 aaaattttaa tgaccttgat ttaccagaaa aaactgttat taatcataag cgatataaga
     3361 tcgttgaata agaaggtaag ttataatgaa taaaatctcg aaggctttaa cttggtttat
     3421 tataagtttc gttatatttc atctcatatt atttatcatg tggggcgaac accaagaata
     3481 ctggtattta tatacaggta taatgttaat tgctggtata agttatgtat tttatcaaag
     3541 agatattgaa tctaagcggc tgcttacatc aattggtatt ggtattatta cagcaattat
     3601 tctaattatg cttcaacttt tattctcact tataacttct aatttaagtt atagttcatt
     3661 aattaaagaa ttagcgagaa cgggtgtcaa ttggaaatgg caaatgttag taacattgct
     3721 ttttgtcatt ccgtgccacg agttatatat gagaactgtt ttacaaaaag aattaacaca
     3781 tttttcatta ccaaaatggg ctgttattat aataactgca gtttgttcta gttcattatt
     3841 catctattta gataattggt ggatagtgac atttatattt gttgctcaag tgatattatc
     3901 gttaagttat gaatatactc gacgtattgc aacaacatcc gttgcacaaa ttgtagctat
     3961 tatattgtta ttaattttca atgcttaaat aagcttttct tagacgtttt tgttctaaga
     4021 aaagcttttt gctttagaat agcaatatga agattcaagg catgcttcat tatagtgtat
     4081 acatttttaa cagcaattgg ttattatcga gtttacattt tagccagttt ttattattaa
     4141 atattttata aaaacggtat aatattttgt gagcaatttg aattgatggt ggctatctga
     4201 gtaaaggggg tggtgcctat ggcattactt aacacttttg aaaggaaaag cctattgtga
     4261 tatctattgc aaacgcatta catttaatgt taagtttcgg tatgtttatc gtaactttca
     4321 ttggtatagt agtcgcaata attaatttaa acaataaaaa ataaccatca tttcaaaact
     4381 ttgacaaaga atgatggtta aagaaaatct catttagtca ccgtcttttt aacgggatca
     4441 ataggcgaca tgttcctgca tgtcgtcttt ttacattttt atagtaacat atcttttttt
     4501 aatctgtcta tttttattag atgtgtaaca aaattttgaa atagtttttg tatattgctt
     4561 gttaaacatg cgatatttgt atggaaaatc atactatgat agaaatcact tatgattcta
     4621 tggtagtact tataaataag tgatttctat tgaatatgta atgaagttca tatattaata
     4681 taactttcat tggtatagta gtcgcaataa ttaatttaaa caataaaaaa taactatcat
     4741 ttcaaaactt tgacaacgaa tgatggttaa agaaaatctc atttagtcac cgtcttttta
     4801 acgggctcaa taggcgactt tttagcacat atcatttttt gtactgcaca atttaataat
     4861 aatacaatta taatttggtg tctacttaac atatcgtact agaaatatat ggcggatgaa
     4921 ttaatcttga ctattttcag aaaattctgt ataaataaca aaaaagagag aaaataaata
     4981 taaagaggtg tgtttaacag aagaacaaaa gttttgactc tttattatat ttatctttag
     5041 cacttggatt attcagagta gcttatgcat ctacagaata tgcagaagga tgcgcgtgga
     5101 ctcacggtgt cggcagtaag tatgtttggt cttattatta tcatggtcat aaaggtcatg
     5161 gtgcaacagc tattggaaaa tacagatcat ttagtggtta tacaagagct ggtgtaaaag
     5221 caaaagcatc agctactaaa aataattggt gggtcaatag agcatattat aacatttatt
     5281 aaatactaaa taatcataca aaacaaacaa cgcaagaggt acaacatgaa atggttcaaa
     5341 ttaatacttg atgttacaat cttcattcta attgctatat tgttatttgt ttatacatat
     5401 aaagaaaacg aagaaatatt gcctgatact aaatacccta tagtggtaac tgactggaat
     5461 aaaaaatata gtaaaaatga gatttataaa catataaatc aattcgctaa aaatgagaac
     5521 gtagcaatct ataaatcaac ttcaaattat acaaataaaa aagtagataa agatatatat
     5581 gtatttaata aatcgaaagc aacacctata acaccgttta actctaaata taacattcac
     5641 tatataaatg acaatgaatt attaaaaaag gatatcaaag ggagttattt tgtcaaagac
     5701 acaaattttg acgtatctaa atttataaat cttttaaaac aatatggtgt ttctgctgaa
     5761 tcatacaaag ttaatcatat gatgattgcg gtaggtgtca ttaaacaaat gaatttagta
     5821 gtgccgttat ccgcactttt aatcgtttat tttatatatt atattttcga aaaaaacatt
     5881 aactttaaag gttatgcgat taagtattta aatggtttta cattaggaaa aataattttc
     5941 gagaagtttt caatgaaatg tacgtattgg ttaacgttaa tgataactca aattatttta
     6001 actacatgca tactttggat tttaaactac acaggtaatt tagatttatt catattaaga
     6061 ttagttcttc tttcaagtct ttttatttta acgattagta gtattaattt atggactttt
     6121 ttaatgttat taaatttaaa tattgccaac atgattaaag gtaagcagca ctttaaaaca
     6181 atacgtttta ttaatacagt ttgtaaaagt attcttttag tactaatatc taccgtaatt
     6241 atagaaaatg ctagcgtaat taaagatttg aataaaataa aagaaactga gaaatattgg
     6301 aatgtattag atgattatta cacggttgaa tttgcacctt atcacgaaac gaaacaaagt
     6361 ttgattgata atatggtgcg atcagagcaa ttagtaaagg ctagtgaagc agaaaataat
     6421 gcgattttat tcaaaccaaa gagtgactcc gctgacagtg acaacttttc gcctgatgag
     6481 gggaatgtaa tattagtaaa taatcagttt tggtcgattt atcacaagca gtttcaacct
     6541 gatattccga taaaaaatca aaaaaataat gtcgaagtaa ttattccaca aaagtttcat
     6601 gcaatgcgta atgaaatcaa tcaagcgtat cattcatggt ttgaatttgt acaaaataaa
     6661 aataataaag aaaataagtt atccatgcag tttataaaca caaatgatta ccgaattttt
     6721 acatttgatg cacgagatag tcgtcatttg tcatttatag attctccaat cattgtgaat
     6781 gttcaggcct cagatttatc caatgatttt tattatgcca tgataagtca aggcggatat
     6841 ttattcaaaa attatgacgc gttagtaaaa aatattgaaa agtatcatct tgatggggaa
     6901 atcagtggaa taactaatta taaagatagc gtaatggaaa tgtatcatga aaacaatctg
     6961 aagttaacag tattcatatt ttcacaaatc attattgcaa tcattttagt aattattatt
     7021 ttatttgatg tgaaatatta ttttgaacag catcgaaaat tactcgtaat caaaaaacta
     7081 tatggctatt caacattaag agcaaattat caatatttat taataaataa tatttttgtt
     7141 gtttttattg gaatattaac gaatgtattt ataaattctc attatttagt gatgatattt
     7201 gcgacaattc tcgtagttca aatactgttg cagttttgta gcttatatta tttagataga
     7261 cgttttaatg aagttatcaa ggagttttaa ctatgaaatt tatcattgca atattattag
     7321 gattaattat ggctattact attgcgttca caattataca ccatccaata cttgatcgtt
     7381 ttaatccttt cttaaaaacg gagtccagct ttgccaaagt gccaaaaggt acgcaacaat
     7441 atgtgaatat tacggcttat agtgaaaaag gggaaaaact tgattataaa ttaacattta
     7501 atggattctc tccaagtaga acatatgtcg aaataaagca taaaggccaa tatgttatat
     7561 cgataacata tgttgaaaga gaggaaacgc caaaagaggt aagacaagaa tgatagaatt
     7621 aaaagattta actatacaaa aaggtaatac aagtatttta aagaaactta acttgaaatt
     7681 tcaatgtgga aaatcatatg cacttattgg taaaagtgga tgtggaaaat ctactttatt
     7741 aaatgtaatt gctggacttg aaaaaatggg gaaacagcat atttatttta atggtcaaga
     7801 agaacgattc aaacctaatt tttataggga taagttaggt tatttattcc aaaattatgg
     7861 actaatcgat aatatgagtg taaatgaaaa tctagagatt ggattagcat ataaaaaaat
     7921 aagtaagaaa gaaaaagaac aattaaaaat atgttattta gaacagtttg gactatatga
     7981 tagtttaaag agaaaagttc atacgttaag tggtggagaa caacaacgcg ttgccctaat
     8041 aagaatgata ttaaaagatc caactattat tttagcagat gaaccaacag gtgcattaga
     8101 tccgaaaaca ggacagttga tcattcaaac attatttaat ttagtcgatg aaaataaagt
     8161 actaatttta gcgacacatg atatggctat tgcaaatcga tgtgatgtag taattgattt
     8221 ggaacagttt aaaaatgtag tatctatgtg atgcaataaa gttatattta aagccaatca
     8281 aaaggaatgt tgaaatgaac atgcccagtt gattggcttt ttgttatgcg ttgttaagtt
     8341 atgaaagtaa caacggcaaa ctgaaaaaaa gtaaaataat aatatttaag acaagcaata
     8401 ttaaatgtaa ttgatttttc gttttaatgt aacaaaaaac aataaaaagt gataacagca
     8461 aaatgatgat tgcgccaggg aagaaccata atgctgaagc agtgtaccaa attattggca
     8521 acgccataat aataaaagat aaaagacaag aaatgatacc aataatatta tatgttctag
     8581 tcaaaattac acctccaaat ttatatttaa aatgatatgc ctaaaacaca cattttaaat
     8641 acatcataca ataaaattaa attgtgcgat agaagaattt gaaattaaat tgaattgtct
     8701 ttggtggaaa tgtaatttac cctaaagtgt atttttatac catgaagaaa aatgtagcaa
     8761 gttaagataa tattcgttgt ttaaaatttt gcgtaatatt attgttttta ttatgcaaat
     8821 aaatataata acaatattta attattgtat ttgtagttga ttaataatgt tacgaagtat
     8881 aaaattattt tgtaaaacaa tttttttatt aagaggtgcg ctatggaaaa ggggattttc
     8941 aattacgata atgcgaaagt attaaaatta gatacgaatc aattaaatga aaatataaaa
     9001 gtcattgata atgtatttaa aaattatgaa cagttagagc caactataga aattgaaaat
     9061 gggaagtcag ttttaaaatt aaatggacat tttattgcgt caattatagg accattaaat
     9121 gtaaataaac ttaataattt atatgttgat gaagattttt atcatacata taacgaatta
     9181 attgttaaat atactgaggt aaaggaataa ttatgatgaa aaaagtattt atgatggtgc
     9241 atgagttaga tgtcaacaaa ggcggtatga cctcttcgat gttcaataga agtaaagagt
     9301 tttatgatgc ggacattcct gctgatatag ttactttcga ttataaaggc aactatgatg
     9361 acattataaa aacattgaaa aaacaaggga aaatggatcg aagaacaaaa atgtataatg
     9421 tatttgaata ttttaagcaa atatcgaata acaaacattt taagtcaaat aaattgttat
     9481 ataaacatat ttcagaaaga ttgaaaaata cgattgaaat tgaagagagt aaaggcattt
     9541 caagattttt tgatatgacg acaggaacat atattgccta cataagaaaa agtaaatctg
     9601 aaaaagtgat tgatttcttt aaagataata aacgaataga acgatttagt tttataggta
     9661 ataaagtgca tatgaaggaa acatttaatg tagataataa ggtttgttat caagtgtttt
     9721 acgatgaaaa gggataccca tatatttcta gaaatattaa cgctaataat ggcgctgtag
     9781 gtaaaactta tgtgttagtt aataaaaaag aatttaaaaa caatctagca ctatgtgttt
     9841 actatttaga aaaactaata aaagacacta aagatagtat tatgatttgt gatggaccag
     9901 gtagttttcc aaaaatgttt aatacaaagc ataaaaacgc tcagaaatat ggtgttattc
     9961 atgttaatca tcatgaaaac ttcgataata ctggtgcatt taaaaaaagt gaaaaatata
    10021 ttattgaaaa tgcgaataaa attaatggtg taattgtatt aacagaggca caacgattag
    10081 atattcttaa gcaatttgat gtagaaaata ttttcactat tagcaatttt gttaaaatac
    10141 ataatgctcc aaaacatttt cagaccgaaa aaatcgttgg tcatatttct agaatggtac
    10201 caacgaaacg aattgattta cttattgaag tagctgagtt agtcgtaaaa aaagataatg
    10261 gtgttaaatt ttatatatat gg
//
