LOCUS       NZ_CP031749            10261 bp    DNA     circular CON 06-NOV-2024
DEFINITION  Bacillus sp. E25 chromosome.
ACCESSION   NZ_CP031749
VERSION     NZ_CP031749.1
KEYWORDS    RefSeq.
SOURCE      Bacillus sp. E25
  ORGANISM  Bacillus sp. E25
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae;
            Bacillus.
REFERENCE   1  (bases 1 to 5915788)
  AUTHORS   Equihua,A., Hernandez-Salmeron,J.E. and Santoyo,G.
  TITLE     Draft genome sequence of an endophytic bacterial strain isolated
            from tomato plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5915788)
  AUTHORS   Equihua,A., Hernandez-Salmeron,J.E. and Santoyo,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2018) IIQB, Unversidad Michoacana de San Nicolas
            de Hidalgo, Santiago Tapia, Morelia, Michoacan 58000, Mexico
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-MAY-2018
            Assembly Method                   :: SPAdes v. 3.10.0
            Assembly Name                     :: Bacillus sp. E25
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: Bacillus sp. E25
            Genome Coverage                   :: 358.587x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003429225.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 01:21:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,042
            CDSs (total)                      :: 5,949
            Genes (coding)                    :: 5,722
            CDSs (with protein)               :: 5,722
            Genes (RNA)                       :: 93
            rRNAs                             :: 10, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 10, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 94 of 227
            Pseudo Genes (incomplete)         :: 145 of 227
            Pseudo Genes (internal stop)      :: 69 of 227
            Pseudo Genes (multiple problems)  :: 62 of 227
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:28:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2849871
            Orig. end                         :: 2860132
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031749.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10261
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="13"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10261
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10261
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5261](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5261
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            977..2338
                     /locus_tag="DPQ26_RS14205"
                     /old_locus_tag="DPQ26_14605"
     CDS             977..2338
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017763222.1"
                     /locus_tag="DPQ26_RS14205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DPQ26_14605"
                     /product="glycosyl hydrolase family 8"
                     /protein_id="WP_098424918.1"
                     /transl_table=11
                     /translation="MNGKRKFFTCISIIGIGLASFSNSSFAANVTDNSVQNSIPVVNQQ
                     VASAKEMKPFPQQVNYAGVIKPTHVTQESLNASVRSYYDNWKKKYLKNDLSSLPGGYYV
                     KGEITGDADGFKPLGTSEGQGYGMIITVLMAGYDSNAQKIYDGLFKTARTFKSSQNPNL
                     MGWVVADSKKAQGHFDSATDGDLDIAYSLLLAHKQWGSNGTVNYLKEAQDMITKGIKAS
                     NVTNNSRLNLGDWDSKNSLDTRPSDWMMSHLRAFYEFTGDKTWLTVINNLYDVYTQFSN
                     KYSPNTGLISDFVVKNPPQPAPKDFLEESEYTNAYYYNASRVPLRIVMDYAMYGEKRSK
                     VISDKVSSWIQNKTNGNPSKIVDGYQLNGSNIGSYSTAVFVSPFIAASITSSNNQKWVN
                     SGWDWMKNKRESYFSDSYNLLTMLFITGNWWKPVPDDKKIQNQINDAIYEGYDN"
     gene            2501..3424
                     /locus_tag="DPQ26_RS14210"
                     /old_locus_tag="DPQ26_14610"
     CDS             2501..3424
                     /EC_number="3.2.2.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016087665.1"
                     /locus_tag="DPQ26_RS14210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DPQ26_14610"
                     /product="nucleoside hydrolase"
                     /protein_id="WP_098900435.1"
                     /transl_table=11
                     /translation="MEKVLFFGDPGIDDSFAIMYGLLHPEIEIVGIVTGYGNVEHIHAA
                     HNAAYILQLANRQDIPVISGATKPLTEEITTYYPEIHGAEGLGPIHVPESVLSIPIYNF
                     GSIAAILVKYGKDLTIVDVGRSTSLAIAFNLWNDLMLNVKAIYFMGGVFLEVGNVTPLA
                     EANVYGDPIASKIVFHQAKNLLIFPLNVTNKAVLTPNVFSYIQANTKNPFHMIMKPMYD
                     YYLSAYQKLNPSIEGPLLHDVVAISGLVNPSFFEFVHRTVNVDTYGDTKGQTFADFRPS
                     SIPEGAHIALEIDEQKFIQDFIKIML"
     gene            3502..3960
                     /locus_tag="DPQ26_RS14215"
                     /old_locus_tag="DPQ26_14615"
     CDS             3502..3960
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102696.1"
                     /locus_tag="DPQ26_RS14215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DPQ26_14615"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_001102680.1"
                     /transl_table=11
                     /translation="MNVQLKVVTRENWEEALKLQVKENQLKFVPSVAVSLAKVYIKPDG
                     DNVEYIPFAIYVGDLMVGFVMHAVVRKTSDMYWINGFIIDQKQQGNGYGKAALQESINI
                     IKNTFKSCKEIRLTVHKDNISAKKLYERYSFKSLEQEYDGEQVYRLFV"
     gene            3991..4338
                     /gene="cutA"
                     /locus_tag="DPQ26_RS14220"
                     /old_locus_tag="DPQ26_14620"
     CDS             3991..4338
                     /GO_process="GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="cutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160776.1"
                     /locus_tag="DPQ26_RS14220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DPQ26_14620"
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="WP_001160789.1"
                     /transl_table=11
                     /translation="MQFTEVKIEVFIPEEYIETLRDELNKIGACKTGEYDHCLSYSSVK
                     GYWRPLDEASPFNGEIGRICEGQECKVEIKCKRELVKDALEVINDIHPYETPMIYIIPI
                     LNDYFEQLYIE"
     gene            4355..4801
                     /locus_tag="DPQ26_RS14225"
                     /old_locus_tag="DPQ26_14625"
     CDS             4355..4801
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="DPQ26_RS14225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DPQ26_14625"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_000836826.1"
                     /transl_table=11
                     /translation="MKSKFHHIVRAVMIKDEKLLVAEYIGHHYFLPGGHVEIGESAENA
                     LIRELREELGVNCSIQQFLGVIENQWQDKEVLHHEINHIFEVESQDLHTDLPPKSSESH
                     LAFQWIDCNKEALNHYEIMPMPLVKELLERKLSDELLNCWISNF"
     gene            5001..5261
                     /locus_tag="DPQ26_RS14230"
                     /old_locus_tag="DPQ26_14630"
     CDS             5001..5261
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071342.1"
                     /locus_tag="DPQ26_RS14230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DPQ26_14630"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_001071342.1"
                     /sec_met_domain="TIGR03601 (E-value: 7.3e-40, bitscore:
                     133.7, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQHELQTLNLNDYQTGNVVYWDQQQSQYPYYYIQDDARRCGG
                     CGGCGGRCGGCGGGRCGGCGGRCVGCAGCFGCFNCWNWWII"
     gene            5363..6379
                     /locus_tag="DPQ26_RS14235"
                     /old_locus_tag="DPQ26_14635"
     CDS             5363..6379
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288887.1"
                     /locus_tag="DPQ26_RS14235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DPQ26_14635"
                     /product="serine hydrolase"
                     /protein_id="WP_098578111.1"
                     /transl_table=11
                     /translation="MYTYEKLISWVENIKEKNQSSAAALCIIKDNKIVLEHYSGYHSNT
                     STSKKVTAPSQFNVASARKSYLGLIIAYALYEGKINSIDDEAIKYFKDFDPILLGKTTI
                     RHLVTHSHGLEETNDGTIFREFEPGQSWAYRDINVRMMTRLIYQLYNKSFPELLKERVF
                     KPAKFQETGWRIQQDENLVEIINNPNEDPISGIGTVNDGTEKNLFVSAREFAQWGNLHL
                     NQGMIHDKQIVPKEVIKIATSLQSPTFINKELPQNGVFWFIQNEPAQLSELGERIPKGS
                     YQILGITGPTILVIPEYNVVVAKMYNKRYNYGGDNYLYYLREFSNLVADTFSNRIRA"
     gene            6411..6554
                     /locus_tag="DPQ26_RS14240"
     CDS             6411..6554
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="DPQ26_RS14240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_000653680.1"
                     /transl_table=11
                     /translation="MKDKVNKNKKQNIKDVNTEQNAIYSDPKDAANMQTVPQPKDFDEI
                     EY"
     gene            6835..8043
                     /locus_tag="DPQ26_RS14245"
                     /old_locus_tag="DPQ26_14640"
     CDS             6835..8043
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="DPQ26_RS14245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DPQ26_14640"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_098578112.1"
                     /transl_table=11
                     /translation="MKQSSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAAKLQKKLGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYINGNLIDNLGVS
                     QAFLYWGIIVLLLVLIGSFFLREAIVSNAVTETLHNDYTPREMMRTKQVYLLFFMLFTS
                     CMGGLYLISMVKDIGVQLVGLSAATAANAVAMIAIFNTVGRIILGTLSDKIGRMKIVSA
                     TFIIIGLSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGAILGGFQPTFIIIGVLSVISFIISILIRPPKAEKKKEKQHLYR
                     KVA"
     gene            8342..8968
                     /locus_tag="DPQ26_RS14250"
                     /old_locus_tag="DPQ26_14645"
     CDS             8342..8968
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237130.1"
                     /locus_tag="DPQ26_RS14250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DPQ26_14645"
                     /product="cadmium resistance transporter"
                     /protein_id="WP_098578510.1"
                     /transl_table=11
                     /translation="MVTTIISSFMAFISTNIDDIFILLVLFSQVKTGVIKKEGRAVPEK
                     TKMKELYIVVGQYLGFSVIIFLSIIGSLSSFFIPISWIGLLGFVPIYMGVKALLSLRSY
                     KRNTVIDNVSGSIFKVASITLGNGADNISIYIPMFASQNFKTNIVTLVIFFCMIAIWCL
                     ISYKLLRAPILAKVLERNCHIIVPIVLICLGMFILFRSHTIELFY"
     gene            9157..9582
                     /locus_tag="DPQ26_RS14255"
                     /old_locus_tag="DPQ26_14650"
     CDS             9157..9582
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706722.1"
                     /locus_tag="DPQ26_RS14255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DPQ26_14650"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_098578511.1"
                     /transl_table=11
                     /translation="MKIVKQWVQEDSDYIREKVIEYNQKHISDEEKKPSEKISFIVKNE
                     KEEIVGGITAITFWHHVHVDFLWVSKDYRHEGYGSKLLKLIEEFAIEKECRLINLDTFS
                     FQAPNFYKKHGYKVIGVSEDHPKGHNRYYLEKRLENI"
     gene            9794..10237
                     /locus_tag="DPQ26_RS14260"
                     /old_locus_tag="DPQ26_14655"
     CDS             9794..10237
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757615.1"
                     /locus_tag="DPQ26_RS14260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DPQ26_14655"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_098578512.1"
                     /transl_table=11
                     /translation="MKKVNVTYALLYDKTNEKILMVKNKGKNGSYYTLPGGAVKFGETL
                     EEAVIREVKEETGLHITVNGIGYISEAFFEERGHHAIFFNFLGEIIDGEINITRPKEIE
                     GITWMELHKAAPHLRIPEQLLNTLQRKETAPYFFNGTIIHQSS"
ORIGIN
        1 gcaattcgta tggggcttga atcttataca cctgagcatc ttactttact acgcttatta
       61 atcgcttcat ttatacttct gttgttttct tttatctaca agttacggct accggattta
      121 aaggatatcc cagcaatttt tatgttcggt gctttaggat tcacttttta tcacattgca
      181 ttaaactacg gcgaaaaaac agtaaatgct ggttctgcaa gtttaattgt ttcggtcaca
      241 cctatagtaa cggcaattct tgcttctata tttttaaacg aaaaaatgaa attaaatggt
      301 tggatcggtg gtgtaattag ttttatagga attgctctca tatcatttag tcaaggagat
      361 agtattcaat taaatagtgg gggattattt atattatttg cagcaatttc agaaagccta
      421 tattttgttt tccaaacttc ttacttaaaa aagtacggct tcttaccatt caccatatat
      481 acaattgtat ctagtactgt atgtatgctt attttcttac cgggaatgta tcaagaaata
      541 ctagcagctc ctcttgaagt gaatgtgagt gttttatatt taggcttatt tccaacagta
      601 ctcccatata tcgcattggc ctatatcata tctcatgctg gtgctgctga ggcaacaagc
      661 tctctatatt taacccccag taactgcatg ttttattgct tggttatggt taggagaagt
      721 gccaacttta gtttcgataa ttggcggagg gattactata cttggtatcg tgattgctca
      781 tataccagta ttaagaaaag aaaaacatag gaatttagca aataatcaat ccgtataatg
      841 taattcaata atgattttat gatacttttt catatagttt ctttcttatg ttgataaata
      901 taatattttg tatttcagtt aaatattgta tgttacattt tatgtagtaa gcattttaaa
      961 aggagatgac aacataatga atggaaaaag aaaatttttc acatgtattt ctattatagg
     1021 aatcggacta gctagttttt ctaattctag tttcgcagca aatgtaacgg acaattcagt
     1081 acaaaattct attcctgtag ttaatcaaca agtagcttct gcaaaggaaa tgaaaccatt
     1141 tccccagcaa gttaattatg caggtgttat aaaacctact catgttacac aggaaagttt
     1201 aaatgcttct gtaagaagtt actacgataa ttggaaaaag aaatatttga aaaatgattt
     1261 atcttcttta cctggtggtt attatgtaaa aggagagatt acaggtgatg cggatgggtt
     1321 taagccactt ggaacttcag aaggtcaagg gtatgggatg ataattacag tattaatggc
     1381 tggttatgat tcgaatgctc aaaaaatcta tgacggttta tttaaaacag caagaacttt
     1441 taaaagctct caaaatccta atttaatggg atgggttgtc gcagatagta aaaaagcaca
     1501 aggtcatttt gattctgcta ctgatgggga tttagatatt gcgtattctc ttcttcttgc
     1561 tcataagcag tggggatcaa atggaacagt taattattta aaagaagcac aagacatgat
     1621 tacaaaaggt attaaagcta gtaatgttac aaataatagc cgactaaatt taggagattg
     1681 ggattctaaa aattcacttg atacgaggcc atctgattgg atgatgtcac accttagagc
     1741 attttatgaa tttacaggtg ataaaacttg gcttactgtt attaataatt tgtacgatgt
     1801 ttatacgcaa tttagtaata agtactctcc aaatacagga cttatttcag atttcgttgt
     1861 aaaaaaccca ccacaacccg cacctaaaga cttcttagag gagtcagaat atacaaatgc
     1921 atattattac aatgctagtc gggtaccatt gagaattgta atggactatg cgatgtacgg
     1981 cgagaaaaga agtaaagtca tttctgataa agtctcttca tggattcaaa ataaaacgaa
     2041 tggaaatcct tctaaaattg tggatggtta tcaattaaat ggatctaata ttggtagtta
     2101 ttcaactgct gtatttgttt caccgtttat tgctgcaagt ataacgagta gcaataatca
     2161 aaagtgggta aatagcggtt gggattggat gaagaataag agagaaagtt attttagtga
     2221 tagttataat ttattaacta tgttatttat tacaggaaat tggtggaaac ctgtacctga
     2281 tgataaaaaa atacaaaatc aaataaatga tgcaatttat gaaggatacg ataattaatt
     2341 aaatttgaga atggttactt aagaaaggaa ttagcatacg ctagttcctt tttaatattt
     2401 tgtaaaacat tatccattct aaaattcgca tagatatact tgcttttttc atatacatag
     2461 ttattgatga ctctatgttt ttatgagggg agagtatttc ttggaaaaag tattattttt
     2521 cggagaccca ggtattgatg actcatttgc cattatgtat ggtttactgc atcccgaaat
     2581 tgaaattgta ggtattgtaa ctggatacgg aaatgttgag catatacatg ctgcacataa
     2641 tgcagcgtat attttacagt tagcaaatag acaagacatt cctgtcatta gtggtgcaac
     2701 aaaaccactt acagaagaaa ttactacgta ctaccctgaa attcacggag cagaaggatt
     2761 agggcccatc catgttccag aaagcgtatt atctattcca atatacaatt ttggtagtat
     2821 cgctgcgatt cttgtaaagt acggaaaaga tttaacgatt gttgatgtag gaagatctac
     2881 ctcgttagca attgctttta atttatggaa tgatttaatg ctaaatgtaa aagcaatata
     2941 tttcatgggt ggtgtttttt tagaagtagg aaacgttacg ccgttagcag aggcaaatgt
     3001 gtatggagat cctatagcaa gtaaaattgt tttccatcaa gcaaagaatt tattaatatt
     3061 ccctttaaat gtaacaaaca aagctgtgtt aacaccaaat gtatttagct atattcaagc
     3121 taatacaaag aatccatttc atatgataat gaaaccaatg tatgactact atttatctgc
     3181 ataccaaaaa cttaatccat ctatcgaagg acctttgcta catgatgtgg ttgcaataag
     3241 tggattagta aatccaagtt tttttgaatt tgtgcatcgt acagtaaatg tagatacata
     3301 cggagatacg aaaggacaaa catttgctga ttttcgccct agctctatac ctgaaggggc
     3361 ccatatagca ttagaaatag atgaacaaaa attcattcaa gattttataa aaatcatgtt
     3421 ataagttgtt gtcgaaaaag ggaattattt caatgcgttg aatataaaaa tacatccctc
     3481 tagataaagg agcttacaat attgaatgtc cagttaaagg ttgttacgag agaaaattgg
     3541 gaagaagcat taaagctaca agttaaagaa aatcagttga aatttgttcc atccgtagca
     3601 gtttcgcttg ctaaagtata tataaaacca gatggtgaca atgtagagta cataccattc
     3661 gctatatacg ttggtgacct tatggttggt tttgtaatgc atgcagttgt aagaaagaca
     3721 tcagatatgt attggattaa cggatttatt attgatcaaa agcagcaagg taatggatat
     3781 ggaaaagcag cattacaaga aagtattaat ataataaaaa atacttttaa atcgtgtaaa
     3841 gaaattagat taaccgtaca taaagataat atctctgcaa agaaactata tgaacgatac
     3901 agtttcaaat cattagaaca ggagtatgat ggcgagcaag tatatcgttt atttgtttaa
     3961 ctattaggaa gtaaaggcgt gaaatgaaat atgcaattta ctgaagtgaa aattgaagtg
     4021 ttcattcctg aagaatatat tgaaacatta agggatgaat taaataaaat tggagcatgt
     4081 aaaacgggcg aatatgacca ttgtctttcc tatagttcag tgaaaggata ttggagaccg
     4141 ttagacgagg catctccttt taatggagaa attggtcgaa tatgcgaagg gcaagaatgt
     4201 aaagttgaaa taaagtgtaa acgagaactt gtaaaagatg ctcttgaagt tattaatgat
     4261 attcatccgt atgaaacacc tatgatttat attataccaa tactaaatga ctattttgag
     4321 caactataca ttgaataact aggaggaata tcaattgaaa agtaaatttc atcatattgt
     4381 acgagcagtc atgataaaag atgaaaaatt attagttgct gaatatattg gccatcatta
     4441 ttttttacct ggtggccatg ttgaaattgg agaatcagca gagaatgcat taataagaga
     4501 gttaagagaa gaacttggag taaactgtag tatacaacaa tttttaggag tcatagaaaa
     4561 tcaatggcaa gataaagaag tgcttcatca tgaaattaac cacatttttg aagtagagtc
     4621 acaagattta catacggact taccaccgaa atctagcgag tctcatttag catttcaatg
     4681 gatagattgt aataaagaag ccttaaatca ttatgaaatc atgccaatgc ctttagtgaa
     4741 ggaattacta gaaagaaagt taagtgatga actactaaat tgttggatta gtaattttta
     4801 aatcacttta tttatagtaa atacgtccat cgaatgaata taaagaaaat aggctgaaaa
     4861 acaaggtaca tcacctagtg taagttcata ttttgttgat gtacgcattt agtctatagt
     4921 cttgtttcta ctttgttcac aagaatttat taactttaat atttgggcgg aatttatatt
     4981 tataaatgga ggggttctta atgaatcagt ttcaacatga actacaaaca ttaaatctta
     5041 atgattatca aactggtaat gttgtgtatt gggatcaaca acaaagtcaa tatccatact
     5101 actacattca agacgatgca cgtcgttgcg gaggatgcgg aggttgtggt ggacgctgtg
     5161 gcggttgtgg cggcggtaga tgtggtggct gtggtggacg ttgcgtaggt tgcgctggtt
     5221 gttttggttg ctttaattgc tggaactggt ggattattta aatatagtac atcattaata
     5281 ttttatatga aagaaaggtg taaatactgt ttatactttt ctttcatgct gtaattttgt
     5341 attagtgtta agggggattt atttgtatac atacgaaaag ttgatttctt gggtagaaaa
     5401 tataaaagaa aaaaatcaaa gttctgctgc agcactttgt attataaaag ataataaaat
     5461 cgtgctagag cattatagtg gttatcactc aaatacatct acaagcaaga aagtaacagc
     5521 accttcacaa tttaacgttg cttctgctag aaaaagttat ttaggattaa taatagcgta
     5581 tgcgctttat gaggggaaaa taaactctat tgatgatgaa gcgataaaat attttaaaga
     5641 ctttgatcct atattgcttg gtaaaacgac gataagacat ttagtaacac attcgcacgg
     5701 attagaagaa acgaatgacg ggacaatttt tcgtgaattt gaaccgggac aatcatgggc
     5761 gtatagagat attaatgtaa gaatgatgac acgtcttatt tatcagctat ataacaaaag
     5821 ttttcctgaa ttgttaaaag agcgtgtttt taaacctgct aaatttcaag aaacaggatg
     5881 gagaatacag caagatgaaa atttggttga aattattaat aatccaaatg aagacccgat
     5941 aagtggaatt ggtacagtaa atgatggtac tgaaaaaaat ctatttgtct cagctagaga
     6001 atttgcgcag tggggcaatc ttcatctaaa tcaaggcatg atacatgata aacaaatcgt
     6061 tccaaaagaa gttataaaaa ttgctacgag tttgcagagt ccaacattta taaacaaaga
     6121 gctaccacaa aatggggtgt tttggttcat ccaaaatgag cctgcacaat taagcgaact
     6181 tggtgaacgt attccaaaag ggtcgtatca aatattaggg attactggac cgactatttt
     6241 agtaatacct gaatataatg tagttgttgc aaaaatgtat aataaaagat ataactacgg
     6301 cggtgataat tacttatatt atttacgtga atttagtaat ttagtcgctg atacatttag
     6361 taaccgtatt agggcataat tactcctata acaaaaaggg gtgattgaaa gtgaaagata
     6421 aagtgaacaa aaataaaaag caaaatataa aagatgtaaa tacagagcaa aatgcaattt
     6481 atagcgatcc taaagatgca gctaatatgc aaactgtacc gcagccgaaa gattttgatg
     6541 aaatcgaata ttaatttttc atccgactat atgatgaatg aaaaatacgt cttaagtctc
     6601 ataacattta agaaattatt tgataacata tatacataag catttgaaaa tgtcttatct
     6661 taacaaagct ttctttagaa atatctgata taacctgcaa aaggcacttc tttatgaagt
     6721 gtctttttgt gtatatgaac ttaattacca ttttccgtac agtatcttgt aatttatgat
     6781 ggatatacta actgtgaaaa ttcatgtcaa gataaaagaa ggggacaagt tagtatgaaa
     6841 caaagttcta taaatccgtt actaattgtt ctaggtacaa tcattgttca aattggcctt
     6901 ggaacaattt atacatggag tttatttaat caaccccttg taagtaagtt tggatggaat
     6961 cttaattcag tagcgataac tttctccata acaagttttt ctttatcatt ctcaacttta
     7021 tttgcagcaa agttgcagaa aaaattagga ctgcgaaaac ttattgctac tgcaggaatt
     7081 gttttaggac tcggcttaat acttagttca caagtttctt ctttaccact attatattta
     7141 ttagctggtg tcgttgttgg ttatgcggat ggaacagctt atattacatc actatctaat
     7201 ttaattaaat ggtttccaaa tcggaaaggg cttatttcag gtatatctgt atcagcgtat
     7261 ggaatgggca gcttaatctt tagatatata aacggaaatc ttatcgataa ccttggtgta
     7321 tcacaagcat tcttatattg gggtattatc gtgttacttt tagtgttaat cggatcgttc
     7381 ttcttacgtg aggcaattgt aagtaatgct gtaactgaaa cattacacaa tgactatact
     7441 ccgcgtgaaa tgatgcgaac gaaacaagta tatctcctat tttttatgtt attcacctcg
     7501 tgtatgggtg gtttgtattt aataagtatg gtaaaagata tcggcgtaca actcgttgga
     7561 cttagcgcag caaccgctgc aaacgccgtt gctatgattg caatctttaa tacagtaggt
     7621 agaattattc ttgggacgct atcagataaa atcggccgaa tgaaaattgt ctctgcaacg
     7681 tttattatta taggattgtc agtctttact ttaagtttta ttccgctaaa ttacggaatc
     7741 tattttgctt gtgtagcaag tgtcgccttt tgcttcggtg gtaatataac tatattccca
     7801 gctattgtcg gagatttctt tggattaaaa aaccatagta caaattacgg cattgtctac
     7861 caaggtttcg gatttggtgc gcttgcagga tcatttattg gagcgatact cgggggattt
     7921 caaccgacct ttattataat cggtgtttta agtgttattt cctttattat ttcgatatta
     7981 attcgtccgc caaaagcaga aaagaaaaaa gaaaaacaac atttgtatcg gaaagtagct
     8041 taattttaaa aagagtcctc atatagggct ctttttatat tatgaaatat ttgattttcc
     8101 tttttactaa ttgtgtagga agaaaagagt tacatcattc aaagtaaaat gatttaaaaa
     8161 ggaataaagt agtttatttc ttaacgaacc aagtagcaga ttagtaaaat aatcatattg
     8221 ttttataaaa gaaccaaaat attcttaaaa actacagtta atcaactttg ttattagaaa
     8281 tgcaatacat ttatagacca ttattttctt ataaatttat aatgaaggta gttggcttat
     8341 attggttaca accataattt cttcttttat ggcattcatt tcaacaaata ttgatgacat
     8401 ttttatactc cttgttttat tttcacaagt aaaaacagga gtaattaaga aagaaggtag
     8461 agcagtcccg gaaaaaacta agatgaaaga actctatatt gttgttggac aatatttagg
     8521 gtttagtgta attattttcc taagtatcat tggttcttta agctcttttt tcattcctat
     8581 atcgtggatt gggttattag gattcgtgcc aatttatatg ggagtgaaag cactattgtc
     8641 acttcgttct tataaaagaa atacagtcat tgataacgtt tcaggttcaa tatttaaagt
     8701 ggcttcgatt acattaggta atggagctga caatatttca atttatatac caatgttcgc
     8761 tagtcaaaat tttaaaacaa atattgttac attagttatc tttttttgca tgatagcaat
     8821 atggtgtctt attagctaca aattgttaag agctcctatt ttagctaaag tacttgagag
     8881 aaattgtcat attattgttc caatagttct aatttgttta gggatgttca ttctttttcg
     8941 cagtcataca attgaattat tttactaata tgtttaaact aacggaagtt tttattaaat
     9001 gaatcgaact acgtataaag ccttattaca gatggagtgt agtagggctt ttctttattt
     9061 tagtcaaatg aaacagtgct ctacgactat taaattagac aggacattat gattattaaa
     9121 ttgaatagat actttaaaag gagcgtgata attaaaatga aaatagtaaa acaatgggtc
     9181 caagaagata gtgattacat aagagaaaaa gtaattgaat ataaccaaaa acatatttcg
     9241 gatgaagaga aaaagccttc agaaaaaatt agttttatag tgaaaaatga aaaggaagaa
     9301 attgtaggtg gaataactgc aattacattt tggcaccacg tgcatgtcga tttcctttgg
     9361 gtttctaaag attatagaca cgaaggatat ggaagcaagt tattaaaact tattgaagaa
     9421 tttgcaattg aaaaagagtg taggctaatc aatttagata cttttagttt tcaggcccct
     9481 aatttttata aaaagcatgg atataaagtg attggagtaa gtgaagatca tccgaaaggg
     9541 cacaatcgat attatttaga aaaaaggtta gaaaatatat agaatcccaa aacaaatcaa
     9601 agcataaaga gcgtacattg aataattact ttcaacatac gttctttttt atatagcaat
     9661 gaaaggttat cggttattaa ctttactaag agcagataaa tgaaatactc tattaatttt
     9721 tttatatttt aaaacgatta aatggtagaa tgtaacaaag acagaaaatt ctcgttattg
     9781 aaggtggact tagatgaaaa aagtaaatgt tacatatgca cttttatatg ataaaacaaa
     9841 cgaaaaaatt ctaatggtaa aaaacaaagg gaagaatggc tcttattaca cgttaccagg
     9901 tggcgcagtt aaatttggag aaacgttaga agaggcagta attcgggaag tgaaagaaga
     9961 aaccgggcta catataactg taaatggtat tggttacatt agcgaagctt tttttgaaga
    10021 gagagggcac catgccattt tctttaattt tttaggtgaa ataatcgacg gggagataaa
    10081 tataacaaga ccaaaagaaa tcgaagggat tacttggatg gaattacata aagcagcacc
    10141 gcatttacgt ataccagaac aattattaaa tacattacaa aggaaagaaa cagcacctta
    10201 cttttttaac ggaacaatca ttcatcaatc ttcataacat taacatatca tgtaaggcgg
    10261 g
//
