LOCUS       NZ_CP031749            10303 bp    DNA     circular CON 06-NOV-2024
DEFINITION  Bacillus sp. E25 chromosome.
ACCESSION   NZ_CP031749
VERSION     NZ_CP031749.1
KEYWORDS    RefSeq.
SOURCE      Bacillus sp. E25
  ORGANISM  Bacillus sp. E25
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae;
            Bacillus.
REFERENCE   1  (bases 1 to 5915788)
  AUTHORS   Equihua,A., Hernandez-Salmeron,J.E. and Santoyo,G.
  TITLE     Draft genome sequence of an endophytic bacterial strain isolated
            from tomato plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5915788)
  AUTHORS   Equihua,A., Hernandez-Salmeron,J.E. and Santoyo,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2018) IIQB, Unversidad Michoacana de San Nicolas
            de Hidalgo, Santiago Tapia, Morelia, Michoacan 58000, Mexico
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-MAY-2018
            Assembly Method                   :: SPAdes v. 3.10.0
            Assembly Name                     :: Bacillus sp. E25
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: Bacillus sp. E25
            Genome Coverage                   :: 358.587x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003429225.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 01:21:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,042
            CDSs (total)                      :: 5,949
            Genes (coding)                    :: 5,722
            CDSs (with protein)               :: 5,722
            Genes (RNA)                       :: 93
            rRNAs                             :: 10, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 10, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 94 of 227
            Pseudo Genes (incomplete)         :: 145 of 227
            Pseudo Genes (internal stop)      :: 69 of 227
            Pseudo Genes (multiple problems)  :: 62 of 227
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:28:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2790150
            Orig. end                         :: 2800453
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031749.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10303
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="12"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10303
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10303
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5303](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5303
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    439..453
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 17.35"
                     /tool="antismash"
     gene            529..1977
                     /locus_tag="DPQ26_RS13915"
                     /old_locus_tag="DPQ26_14300"
     CDS             529..1977
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (145-469). E-value:
                     1.7e-30. Score: 98.2. Matches aSDomain:
                     nrpspksdomains_DPQ26_RS13915_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016108507.1"
                     /locus_tag="DPQ26_RS13915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DPQ26_14300"
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_000471637.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1.9e-28, bitscore:
                     98.1, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFKDFKVVKDRPVYIQLKDYLKKMIMKGHLLGNQKIPSTRELSEL
                     LSVSRNTVLAAYADLEQEGLIYAVKGKGNFVAKVDISNTSSVEIDWKNKLNTVTALADE
                     LDLMKHGVRWEKGMIVFNSIAPDEKLFDVENFKRAFLTRMSIEGDIVLNYGYAKGYKPL
                     MNYLLHYMEMKGVDISNKDILITNGFTEGLDIVLSSLSKKSGRVICENPTHHAALKLFR
                     LHGLEVHGIDMNEDGIDTNEVEKSLREKDFDFAYLIPSYHNPTGIVTSSEKRTELMRLF
                     SKYKVPIIEDGFNEELRYSGSHLAPLLTFAGAGNNVIYISSFSKVLFPGLRVGWIIADK
                     ELIHYLESVKRARTIHTSTLDQAVLFQYLHEGYFEKYLKKARSVYKKKYELAVGACNQF
                     IPFKRMTGDGGLHLFIELEEHMNARTLLQKCYEKGVTFSPGDVFYSDGEGANTFRLGFS
                     RLKEEEIVRGIKIIGDTLKNEIWS"
     aSDomain        964..1935
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DPQ26_RS13915_Aminotran_1_2.1"
                     /evalue="1.70E-30"
                     /label="DPQ26_RS13915_Aminotran_1_2.1"
                     /locus_tag="DPQ26_RS13915"
                     /protein_end="469"
                     /protein_start="145"
                     /score="98.2"
                     /tool="antismash"
                     /translation="IEGDIVLNYGYAKGYKPLMNYLLHYMEMKGVDISNKDILITNGFT
                     EGLDIVLSSLSKKSGRVICENPTHHAALKLFRLHGLEVHGIDMNEDGIDTNEVEKSLRE
                     KDFDFAYLIPSYHNPTGIVTSSEKRTELMRLFSKYKVPIIEDGFNEELRYSGSHLAPLL
                     TFAGAGNNVIYISSFSKVLFPGLRVGWIIADKELIHYLESVKRARTIHTSTLDQAVLFQ
                     YLHEGYFEKYLKKARSVYKKKYELAVGACNQFIPFKRMTGDGGLHLFIELEEHMNARTL
                     LQKCYEKGVTFSPGDVFYSDGEGANTFRLGFSRLKEEEIVRGI"
     gene            1982..2896
                     /locus_tag="DPQ26_RS13920"
                     /old_locus_tag="DPQ26_14305"
     CDS             1982..2896
                     /EC_number="6.3.2.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002038752.1"
                     /locus_tag="DPQ26_RS13920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DPQ26_14305"
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="WP_002162788.1"
                     /sec_met_domain="Dala_Dala_lig_N (E-value: 1.2e-21,
                     bitscore: 75.6, seeds: 508, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp_3 (E-value: 1.1e-13, bitscore:
                     50.0, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="RimK (E-value: 1.2e-08, bitscore: 33.2,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 3.8e-53,
                     bitscore: 178.4, seeds: 12, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp (E-value: 1.2e-05, bitscore:
                     23.4, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEILPITLNEKMD
                     LIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIPYSGSNMLSSSICMDKNISKKILRY
                     EGVETPDWIELTKMEDLKLDELDKLGFPLVVKPNSGGSSVGVKIVYNKNELISMLETVF
                     EWDSEVVIEKYIKGDEITCSILDGKQLPIVSIRHAAEFFDYNAKYDDTSTVEEVIELPA
                     EINERVNKASLACYKALKCSVYARVDMMVKDGIPYVMEINTLPGMTQSSLLPKSAEAAG
                     ISYSKLLDMIIETSLKVRKEEGF"
     misc_feature    complement(2919..2931)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.27"
                     /tool="antismash"
     regulatory      2970..3075
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     gene            3203..3892
                     /gene="tenA"
                     /locus_tag="DPQ26_RS13925"
                     /old_locus_tag="DPQ26_14310"
     CDS             3203..3892
                     /EC_number="3.5.99.2"
                     /GO_function="GO:0050334 - thiaminase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="tenA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144490.1"
                     /locus_tag="DPQ26_RS13925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DPQ26_14310"
                     /product="thiaminase II"
                     /protein_id="WP_000144509.1"
                     /transl_table=11
                     /translation="MTFSQSLRKEVDSIWEASFNHPFVKKLGEGTLDLASFRYYVLQDS
                     YYLSHFARVQTLGAAKALELETTARMAHHAQNTYEAELSLHENFAKKLGITKEEKDNFI
                     PAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGERLKECQPEEPIYNEWISAY
                     GSDWFRTLVEEQITRLDTIAEKVTEADRKRMKQHFIISSQYEYSFWEMAYTLEKWPVDT
                     DVKDVIG"
     gene            4290..4553
                     /locus_tag="DPQ26_RS13930"
                     /old_locus_tag="DPQ26_14320"
     CDS             4290..4553
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002101034.1"
                     /locus_tag="DPQ26_RS13930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DPQ26_14320"
                     /product="DUF3937 domain-containing protein"
                     /protein_id="WP_002082716.1"
                     /transl_table=11
                     /translation="MNFERCYMFTNKKLIRFGLSLFVFLGIINFTISYFQTYLETAADI
                     KWVIPEIWKTILLDVPQGILVLLGAIALYDFTKEASQKDASI"
     gene            5001..5303
                     /locus_tag="DPQ26_RS13935"
                     /old_locus_tag="DPQ26_14325"
     CDS             5001..5303
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002059557.1"
                     /locus_tag="DPQ26_RS13935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DPQ26_14325"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_098578256.1"
                     /sec_met_domain="TIGR03601 (E-value: 2.6e-40, bitscore:
                     135.2, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKMNQFQQEIQALSLNDYRSGNIVYWDQQNQYPYYYIEYAARRCG
                     GCGRCGGCGGFRCGGFRCIGCFGCFGCGGCGGCSNCFNGFSGTTDTTGTIITYEY"
     gene            <5456..5587
                     /locus_tag="DPQ26_RS13940"
                     /old_locus_tag="DPQ26_14330"
                     /pseudo=""
     CDS             <5456..5587
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016077325.1"
                     /locus_tag="DPQ26_RS13940"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="DPQ26_14330"
                     /product="site-specific integrase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MYVPTKNIVIINGYIQSFK"
     misc_feature    complement(5747..5765)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.87"
                     /tool="antismash"
     gene            5797..6282
                     /locus_tag="DPQ26_RS13945"
                     /old_locus_tag="DPQ26_14335"
     CDS             5797..6282
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016124614.1"
                     /locus_tag="DPQ26_RS13945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DPQ26_14335"
                     /product="hypothetical protein"
                     /protein_id="WP_002082715.1"
                     /transl_table=11
                     /translation="MNMVELKSIFHSYKVKRKIAKDLYGKRDELTLLLNEFNNMKCTVT
                     SEKKKNNILSRLQLIYQNIKLDKQYPLSVSFDSKLLERLEKESLHTIEDGVTCLHLMLD
                     MNYEKIKQYGSSTSRSFVPLSQSSICLADCICLTGFIVGLLGVISFGGFMLSICSTT"
     misc_feature    complement(6331..6344)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 20.67"
                     /tool="antismash"
     misc_feature    6332..6345
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 20.94"
                     /tool="antismash"
     assembly_gap    6390..6439
                     /estimated_length=unknown
                     /gap_type="within scaffold"
                     /linkage_evidence="align genus"
     misc_feature    complement(6736..6749)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.22"
                     /tool="antismash"
     misc_feature    6791..6804
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.3"
                     /tool="antismash"
     gene            6796..9183
                     /locus_tag="DPQ26_RS13950"
                     /old_locus_tag="DPQ26_14340"
     CDS             6796..9183
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736192.1"
                     /locus_tag="DPQ26_RS13950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DPQ26_14340"
                     /product="RNaseH domain-containing protein"
                     /protein_id="WP_081143677.1"
                     /transl_table=11
                     /translation="MKKLRLLTFENIVEPLLNEKVSFIYFPIEWLDIVEIHYKTFLLTS
                     KLKRLNERLYDMFSDILFIQHNPYVLNENTPWIVSKEPIKQEQLNYIFQSWYEIIHDWK
                     PNKLVEPPKYEWQSDLISNLPVLHDNETYSKWVPALISHIFCERPIYLENTNAEEIYFS
                     PLRSQNICEAMSGPIKDEKTQDFFSYVYRFECITRGGENAPLLNISIGIRRFYQEYNHP
                     DIPLLFRRKRGMILISTPEFASNNKKIRFVKLKMQQATTGMKWIKIFRNLKDDFQIGGD
                     LDLDHILQYPKDYIVGENKRVLFPYNERIYKVQGTKIEPGIKVKEREYLFKEFQRKFSY
                     FTILPECKKVATNNKNELFPLFAPKGLETLTLEVWSENIALEIEQALLDSEIVLTKIGE
                     NSYVLNTDFPVLLKIVRYNIEKVLQNPYKMQYCQKYKTNLVDDVTKAVYATAGERSDAT
                     LALIAMKNCDGREKIDPKQIIREGFARTNRISTFINLFIGQSVSRKTIVNSVFSLLEQK
                     GFLKRSWNKINLPCTYVNLSIERISKFDFLPILSKIKGKEILYKLYGNTEWQKIDYVLL
                     NINKHNAFLSQPSKRNDMGALFKQYVFETLMEIVQYAKEQNEQVYFIVDANLRKYWIKE
                     LQNKEINTDILPDIVPEVLKVPNLNIIRINTGFDVPSYGVIECDDALDRIGLYADQKGM
                     YYSTGEYSLNCSEVFQRYIIEILPLGVKPVERDYIAKMIHYMCCNSSMLSKKNVHMTYS
                     VHMEKVIKSYITDIDAREFKEFDDELDVDVVKVEKKDSIILL"
     gene            9391..10158
                     /locus_tag="DPQ26_RS13955"
                     /old_locus_tag="DPQ26_14345"
     CDS             9391..10158
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017560251.1"
                     /locus_tag="DPQ26_RS13955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DPQ26_14345"
                     /product="DUF3959 family protein"
                     /protein_id="WP_014894749.1"
                     /transl_table=11
                     /translation="MKKLDVLLMLLSVLFPIAGLTKQIPLEQSVYIGGLLFFTSFGSYF
                     AKKMYSRICSWIAYAPFITLLLVIWHQDITTSSIIANAKIAACVALIPCLFRFRTYGLT
                     LGLFSLWAALLWDIKEVQSLVILERMTSLMTSHYVYILLLIGGLVLGGLLAMFIHRKEK
                     DNNKENINLLGPKKKRKRLSFKIRLPRLPKLKMKLFKFGGKESKSKIPEKIHEHKYEEP
                     VATYKMTEKIDPYKENEVQGQTRMERRRNRFNA"
ORIGIN
        1 agcactgaaa gaagcgcaaa ataaaggaat tgctgagaca aaccctatat tagatataag
       61 tggttcagat agtgcgtgta aattgttact tttgacaaac agcttaatgg aaacagagaa
      121 aacacttact gatatacata taaaaggaat cgagcacgtt acaaaacaac aaatacaaaa
      181 tgctaaggaa caacataaaa ttattaaatt aatagcatca atatataagg atgagggtgg
      241 caatgtgaat ttaaatgttg aaccgtgcga aatagaaaaa gatcatccgt tagcaaaagt
      301 aaatgggacc gaaaaaggaa ttacgttctt tacagatacg atgggacaag ttactacaat
      361 tggtggggct tctaatccac gaggcgctgc agctgcagct ttaaaagatg taattaactt
      421 atatcgaaaa gacttgtaag accgtaccgc cctttataaa aaatacatat gtcgtggacc
      481 ttttgtgaat gaaataaata tgcaaaaatg attgcagggg gtaaggaatt gtttaaggat
      541 tttaaagttg ttaaagatcg tcccgtttat attcaattga aagattattt gaagaagatg
      601 attatgaaag ggcacttgct cgggaatcaa aaaatcccat caacaaggga actaagtgaa
      661 ttactaagtg tgagtagaaa cactgtactt gctgcttatg cagatttaga gcaagaagga
      721 ctcatttatg cagttaaagg aaaagggaac tttgttgcga aggtagatat atctaacact
      781 tcgtctgttg aaatagattg gaaaaataaa cttaatacag tgaccgcatt agcggatgaa
      841 ttagatttaa tgaaacatgg tgttcgttgg gaaaagggaa tgattgtttt taatagtata
      901 gccccggatg agaagctatt tgatgttgaa aacttcaaaa gagcttttct tactcgtatg
      961 tccattgaag gggacatcgt attgaactac ggatatgcaa agggatataa accgttaatg
     1021 aattatttac ttcattacat ggaaatgaaa ggtgtagaca tttcgaataa agatattcta
     1081 attacaaatg gatttacaga aggattggat attgtacttt cttctttatc aaaaaaatcc
     1141 ggtcgtgtca tttgtgagaa tccaactcac cacgctgcac taaagctgtt ccgtttacat
     1201 ggacttgaag ttcatgggat tgatatgaat gaggatggta ttgatacgaa tgaagttgaa
     1261 aaaagcttac gtgaaaaaga ttttgatttt gcttatttaa ttccttctta tcataatcca
     1321 accggtattg ttacgagttc agagaaaaga acggaattga tgaggttatt ctcgaaatat
     1381 aaggttccta ttatagagga cggatttaat gaagaattac gttattcagg ttcacattta
     1441 gctcccttat taacttttgc tggagctggc aataatgtga tttatattag tagcttttca
     1501 aaggtacttt tccctggttt acgtgtaggt tggattattg cagataaaga gctgattcat
     1561 tatttagaaa gcgtaaaaag agcaagaaca atccacacat ctacgttaga tcaagcggtt
     1621 ctatttcaat atttacatga gggatatttt gagaaatatt taaaaaaggc aagatcggtt
     1681 tataagaaaa aatatgagtt agctgtcggc gcgtgtaatc agttcattcc tttcaaaaga
     1741 atgactggag atggtggact tcatttattt atagagctag aagagcatat gaatgcccgc
     1801 acacttttac aaaagtgtta tgagaaaggc gttacgtttt cacctggaga tgttttttac
     1861 tctgatggag aaggggcgaa cactttccga ttagggtttt cgcgcttgaa agaagaagaa
     1921 atagttcggg gaattaaaat aattggtgat acattaaaaa atgaaatttg gagttgagaa
     1981 aatgaaaatc ggtgttatta tgggcggggt atcgtctgaa aaacaagttt caattatgac
     2041 agggaatgaa atgattgctc atttagataa aaataagtat gaaatactcc caattacatt
     2101 aaatgaaaaa atggatttaa ttgaaaaagc gaaagacatt gactttgcgt tgctcgcatt
     2161 acacgggaaa tatggagaag atgggacagt tcaaggaaca ctggagagtt tagggattcc
     2221 ttatagcgga agtaatatgt tatctagtag tatatgtatg gataagaata tttcaaaaaa
     2281 gattttacgt tatgaaggtg tagaaacacc agattggatt gaacttacaa aaatggagga
     2341 tctaaagctt gatgagttag ataagttagg atttccatta gtggtaaaac caaactctgg
     2401 cggatcgagt gtcggggtga aaatcgttta caataaaaat gaattgatct caatgctaga
     2461 aactgtattt gaatgggatt ctgaagtagt aattgagaag tatataaaag gtgatgaaat
     2521 tacatgttcc attctggatg gaaaacagtt acctatagtt tcaattcgac atgcagccga
     2581 gttctttgac tacaatgcaa aatatgatga tactagtaca gttgaggaag ttatcgaact
     2641 tccagcggaa attaatgaac gtgtaaataa agcgtcactg gcttgttata aagcattaaa
     2701 atgtagtgtt tatgcaagag ttgatatgat ggtgaaggat ggaattccat atgtaatgga
     2761 aattaataca ttaccaggga tgacacaatc aagtttattg ccaaaaagtg cagaagcagc
     2821 gggaattagt tatagcaaac tattagatat gattattgaa acatcattaa aagttagaaa
     2881 agaagaaggg ttttaagggg attatataac taattctttt gacagtttca cgaagtatgc
     2941 tatgatgaat acattataaa aacaaaatat ttatccacta ggggggcctt ttataggctg
     3001 agatcaaatg tgattttgag actcttagta cctgatctgg ttaatgccag cgtagggaag
     3061 tgggaaagac gttgctattt aatgagtaaa taaatactgt caatttcact ttctttacgc
     3121 ctgtaaagaa agttttttta ttttacagct ttaataattg tggataaata cttatatttt
     3181 atacattatt ggagggattt aaatgacttt ttcacaatca ttacgtaaag aagtagattc
     3241 aatttgggaa gcgagtttta accatccttt tgtaaaaaaa cttggtgaag gaacgttaga
     3301 tttagctagt tttcgctatt atgtgcttca agattcatat tatttgagtc attttgctag
     3361 agtacaaact ttaggagctg caaaagctct tgaattagag acgacagctc gtatggcaca
     3421 tcatgctcaa aatacatatg aggccgagtt atctttacat gagaattttg ctaaaaagct
     3481 agggataaca aaagaagaaa aagataattt tattcctgcg ccaactgcat atgcgtatac
     3541 ttcacatatg tatcgtgctg cgtatgaagg gcatttagga gatatcattg cagcaatttt
     3601 accttgctat tggttgtatt acgaaattgg ggaacgttta aaagaatgtc agccggaaga
     3661 gccaatttat aacgaatgga tttctgctta tggatctgat tggttccgta cgctagttga
     3721 agaacaaatt acacggttag atactattgc tgaaaaagta acagaggcag accgcaagcg
     3781 tatgaaacag cattttatta ttagcagtca atatgaatat tcattttggg aaatggctta
     3841 tacattagaa aagtggccag tggatacaga cgtaaaagac gtaattggat aaagaataga
     3901 agtaatgtta gagataggta tggatcatac ctatctctat attttttctt gtcaaatttg
     3961 cgtaaagtca ttagtgctac ggatatggtg aaatagaata tcgataattt agctcattta
     4021 attgtctacg cattaactgg ataaacttct gtcacattta ttttacaatt aaagaacgta
     4081 aatgttggtg gtatgaagtt aaataagaaa agaaacccga gctgttagct gtaacggagt
     4141 tgttttttct ttgcaggaat ttctaagtta acatgaagtg ctgttcatac aatatgcaaa
     4201 tttgcatatt gtattttttt gagttctttg tgtgttaaaa tcaaatatag agatatagaa
     4261 atataaagaa gcaaaagcag gaatatccaa tgaattttga gaggtgttat atgtttacaa
     4321 ataagaaatt aattcgattt ggtttatcgt tgtttgtatt tttagggata attaatttta
     4381 caatcagcta tttccaaaca tatcttgaaa cagcagcaga tattaaatgg gtaattccag
     4441 aaatttggaa aactatttta ctagatgttc ctcaaggtat acttgttctt ttaggcgcaa
     4501 ttgcattata tgatttcaca aaagaggcat cacaaaaaga cgcatcaatc taagtgtgtc
     4561 ttttttcaat tagaaatttc atagagagct ttgctggaga aatagaataa aaagccctaa
     4621 ttagggcttt tttctttata ggaatttctt aaccttcata gaaaccggtt acttagaggt
     4681 gttatagcaa aggcatttac agtacaagaa aatctaagtg atatatggaa atatatagga
     4741 tttgtccgcg tgattattga gaaattaatt gagcttagta agtatgaaga tatgacgcta
     4801 gaatttatat tttataattt ctatttataa aaatgagccc atagtaaaaa taggtatatg
     4861 aacaaggtgc atcatctagt aataagtcat actttgttgg tgtacgtatt gaacttagtt
     4921 tatagcgcta tttctactta gatataacga acagaaatat agggaagcat caatgaggtt
     4981 gtattttata aacggaggtt atgaaaatga atcagtttca acaagaaata caagcgttaa
     5041 gccttaatga ttaccgatct ggaaatattg tctattggga ccagcaaaat caatatccat
     5101 attactatat tgaatatgct gctcgtcgct gtgggggttg tggtcgttgt gggggttgtg
     5161 gcggattccg ttgtggtgga ttccgttgta ttggttgctt cggttgtttt ggttgcggag
     5221 gttgtggcgg ttgctctaac tgttttaatg gttttagtgg tactactgat actactggta
     5281 ctattataac gtatgaatat tgattgcatt tatcctgcta tttgccgggc agtaagaccc
     5341 taaaaaaatt cagcgagagc aaagagaagt taggtggccg gctgcccata aaagcccgat
     5401 ggatgagggc taattaaagt ttcaccttat caattaaaaa acattagttt tatatatgta
     5461 cgtgccgaca aagaatatag tcattattaa tggatatatt caatcattca agtgaaagag
     5521 taacaataag atatataggt gtaatcaaga tgtgatggat aaagcaatga ctagatttaa
     5581 aatctaatca ttgttttttt cttatggttt tatcattaaa tatctgtcta gtttgggcat
     5641 tgcttccttc tagctaatga ttttattaca accgtgtaag gttaatgtaa tgttaatcga
     5701 tagtaaaata agttgcttga gtgtatttta aataagatta aaaagtactg tttatctata
     5761 aaataattgg gtaaataata tgtaattagg aagtgtatga atatggttga actaaaatct
     5821 attttccatt cgtataaagt aaagagaaaa atagctaaag atttatacgg aaaaagagat
     5881 gaattgacac tgttattaaa tgaatttaat aatatgaaat gtacagtaac atctgaaaag
     5941 aagaaaaata atatattatc tcgtttgcaa ctaatttatc aaaatataaa attagataag
     6001 cagtatcctc tatcagtttc ttttgatagt aaattattgg agcgattaga aaaagaatct
     6061 ctacatacta ttgaggatgg tgtaacatgt ctacatttaa tgttagatat gaattatgaa
     6121 aaaataaaac aatatgggtc gagtacaagt aggtcattcg ttccattatc gcagtcttct
     6181 atttgtcttg ctgattgtat ttgtttaaca ggatttatag taggtttact aggagtaatt
     6241 tcatttggag ggttcatgtt atctatatgt tcaactacat aacgcttgat atttgtaaaa
     6301 agagcaccga tttgaactgc accccaattg ttagacacag tctaacaatt ggaggtgcag
     6361 tttttctatg gctaaattta cagctgatgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnng cccggtacaa taccggactc acttttatca agctgcctaa
     6481 tcaaataacc gtgtctaact tttaggggtc acttcaattt aaggtgttct tttttggttt
     6541 ttctgtaaag ttattgtaaa tgataagtac aatatattat atatattata taatttaaat
     6601 gtattaaatt acgaatacta agaggtgttg tagataataa ttacttttaa tttttaaaag
     6661 agtcggtttg ataatgtttt atgtagatag tttttacttt tcttttaaat actttgttta
     6721 aatggattag attaagtaac ttttgttcta gctttttgat aatcaagaca actgaagaaa
     6781 ggaaagaatc attttatgaa aaaattaagg ttgttaacat ttgaaaatat agtagaacct
     6841 cttttaaatg aaaaggtatc atttatatac tttcctattg aatggctgga catcgtagag
     6901 atacattata agacgttttt attaacgagt aagttgaaac gtttgaatga aagactgtat
     6961 gatatgtttt ctgatatatt gtttattcag cataatccgt atgtattaaa tgaaaataca
     7021 ccatggattg tatcaaaaga acctattaaa caagagcagc tcaattatat ttttcaaagt
     7081 tggtatgaga ttattcatga ttggaaacca aataaattag tagagccacc aaaatatgaa
     7141 tggcaatccg atttgatttc taatttgcca gtactacatg ataatgaaac gtattctaag
     7201 tgggtgcccg ctttaatctc acatattttt tgtgagcgtc ctatatattt agaaaataca
     7261 aatgcagaag aaatctattt ttctcctctt agatcacaaa atatttgtga ggcgatgtca
     7321 gggccaataa aagatgaaaa aacacaagat tttttctcct atgtatatcg attcgaatgc
     7381 ataactcgtg gtggtgagaa cgctccatta ttaaatattt caatagggat tcggagattt
     7441 tatcaagaat ataatcatcc agatattcct ttacttttca gacgaaaacg agggatgata
     7501 ttgatttcta ccccagagtt tgcatcaaat aataaaaaaa tacggtttgt aaaactaaaa
     7561 atgcaacaag ctacaaccgg catgaaatgg attaagatat ttagaaattt aaaagatgat
     7621 tttcaaatag gtggagattt ggatttagac catattctcc aatatccgaa agattatatt
     7681 gtcggggaaa ataaaagggt gttatttcca tacaatgaaa gaatatataa agtccaaggt
     7741 actaaaatag aaccaggtat aaaagtaaag gaaagagagt atttatttaa agaattccag
     7801 cgtaaattct cttatttcac aatacttcca gaatgtaaaa aggtagcaac aaataacaaa
     7861 aatgaattat ttcctttatt tgcaccaaaa ggattagaga cattaacatt agaagtttgg
     7921 tctgaaaata tagcgctgga aatagaacaa gcattacttg atagcgaaat tgttttgact
     7981 aaaattggtg aaaattctta tgtattgaat acagatttcc cagtattatt aaagattgtg
     8041 agatacaata ttgaaaaggt gttacaaaat ccatataaaa tgcaatattg tcaaaaatat
     8101 aaaacaaatc ttgtagatga tgttacgaaa gctgtctatg ctaccgcggg tgagcgcagt
     8161 gatgcgacat tagcattaat cgcaatgaaa aattgtgatg gacgcgaaaa aattgatcca
     8221 aaacaaatca ttagagaggg atttgcacga acaaatcgta tttcaacatt tattaattta
     8281 tttataggcc aaagtgtatc tcgtaaaaca attgtaaata gtgtttttag tctattagaa
     8341 caaaaaggat ttttgaagcg tagttggaat aagataaatt taccttgtac atatgttaat
     8401 ttatcaattg aacgaatatc gaaatttgat ttcttaccta ttctttcaaa aataaaagga
     8461 aaagaaattt tatataaatt atatgggaat acagagtggc aaaagattga ttacgtatta
     8521 ttaaatataa ataaacataa tgcattttta tcacaaccat caaagagaaa tgatatgggg
     8581 gctcttttta aacaatatgt tttcgaaaca ttaatggaaa ttgtacaata tgcaaaagaa
     8641 caaaatgaac aagtttactt cattgtagat gcaaatttga gaaaatattg gataaaagag
     8701 ttacaaaata aagaaattaa tacagatatt ttacctgata ttgttccaga ggtattaaag
     8761 gttcccaact taaatattat tagaataaat acaggttttg atgtaccaag ctacggtgta
     8821 atagaatgtg atgatgctct agatagaata ggcttatatg cagatcaaaa agggatgtat
     8881 tatagcactg gtgaatattc acttaattgt agtgaagttt ttcagcgata tataattgaa
     8941 attctaccat taggtgtaaa acctgtagaa agggattata tcgctaaaat gatacattac
     9001 atgtgctgta attctagtat gctttcgaaa aagaatgtac acatgacata ttccgtacat
     9061 atggaaaaag taataaagag ttatattact gacatagatg caagagaatt taaagaattt
     9121 gatgatgaat tggatgtaga tgtagtaaaa gtagaaaaga aagattcaat tattctactt
     9181 taaatactag taaacaaatc ctgctcgtta tatcgatcag gatttgtcat ttttttatgc
     9241 aaataaggat atcatttgtc aagtatcttt tcgtttacat atttacttta tgtgcagaca
     9301 ggattaatat gttttttaga aatacaaaaa ctataccaaa tggaaaagcg atttttatta
     9361 attgaaaaaa tttgaaagga atggggaatg gtgaagaaat tagatgtgct cttaatgcta
     9421 ctgagcgtcc tctttccaat tgcaggactt acaaagcaaa tccctctaga acaatctgta
     9481 tatattggag ggcttttatt ttttactagt ttcggtagtt attttgctaa aaagatgtat
     9541 tcacgtatat gcagctggat agcgtatgcg ccatttataa cattactgtt agttatttgg
     9601 catcaggata ttacaacaag ctcaatcata gcaaatgcga aaattgctgc atgtgtcgcg
     9661 ttaattccat gtttatttcg ttttcgtaca tatggactta ctttgggatt attctcatta
     9721 tgggccgctt tactatggga tataaaagaa gtacagtcgt tagtcatact tgaacgcatg
     9781 acgagtttaa tgacaagcca ttatgtatat atattactat taattggagg acttgtatta
     9841 ggaggattac ttgcgatgtt cattcaccgc aaagagaaag ataataataa agaaaatata
     9901 aatctacttg gaccaaaaaa gaaacgcaaa agattatcat ttaagattcg tcttccgaga
     9961 ttaccgaaat taaagatgaa actatttaag tttggtggga aagaatctaa aagtaaaatt
    10021 ccagaaaaga tacatgagca taagtacgaa gaaccagttg caacatataa aatgacagag
    10081 aaaatagatc catacaaaga aaatgaagtt caaggtcaaa caaggatgga acgccgaaga
    10141 aataggttta atgcataatg aagtttaata accccgtaat gacttatctt aatattgata
    10201 aatcattacg gtttttttat gtaattaaat aaaggttgct aatgaggggg gggatagcat
    10261 attttatagg tgagaagtta aataaaataa ggggaggata aaa
//
