LOCUS       NZ_NMOM01000021        10262 bp    DNA     linear   CON 22-JUN-2024
DEFINITION  Bacillus subtilis strain PT26A contig_22, whole genome shotgun
            sequence.
ACCESSION   NZ_NMOM01000021
VERSION     NZ_NMOM01000021.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 10262)
  AUTHORS   Li,J., Zhang,Y. and Wang,N.
  TITLE     Bacillus subtilis PT26A genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10262)
  AUTHORS   Li,J., Zhang,Y. and Wang,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2017) Microbiology and Cell Science, Citrus
            Research and Education Center, University of Florida, 700 Experiment
            Station Road, Lake Alfred, FL 33850, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2015
            Assembly Method                   :: CLC NGS Cell v. 6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 170.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003399995.1-RS_2024_06_22
            Annotation Date                   :: 06/22/2024 02:15:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,652
            CDSs (total)                      :: 4,563
            Genes (coding)                    :: 4,421
            CDSs (with protein)               :: 4,421
            Genes (RNA)                       :: 89
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 77 of 142
            Pseudo Genes (incomplete)         :: 71 of 142
            Pseudo Genes (internal stop)      :: 30 of 142
            Pseudo Genes (multiple problems)  :: 33 of 142
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:54:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 10262
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NMOM01000021.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..10262
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:>10262](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..>10262)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            complement(<1..3647)
                     /gene="srfAB"
                     /locus_tag="CFI07_RS23850"
                     /pseudo=""
     CDS             complement(<1..3647)
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: AMP-binding (12-415). E-value: 9e-116.
                     Score: 378.8. Matches aSDomain:
                     nrpspksdomains_CFI07_RS23850_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (521-589). E-value: 9.6e-26. Score:
                     81.8. Matches aSDomain: nrpspksdomains_CFI07_RS23850_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (605-892). E-value:
                     3.6e-106. Score: 346.5. Matches aSDomain:
                     nrpspksdomains_CFI07_RS23850_Condensation_LCL.1"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="CFI07_RS23850"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="AMP-binding (E-value: 5.8e-127, bitscore:
                     412.0, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 5.6e-18, bitscore:
                     53.8, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 7.8e-101, bitscore:
                     325.4, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQITEATFAALFEKQAQQTPDHPAVKAGGNLLTYRELDEQANQL
                     AHHLRAQGAGNEDIVAIVMDRSAEVMVSILGVMKAGAAFLPIDPDTPEERIRYSLEDSG
                     AKFAVVNERNMTAIGQYEGTIVSLDDGKWRNESKECPSSISGSRNLAYVIYTSGTTGKP
                     KGVQIEHRNLTNYVSWFSEEAGLTENDKTVLLSSYAFDLGYTSMFPVLLGGGELHIVQK
                     ETYTAPDEIAHYIKEHGITYIKLTPSLFHTIVNTASFAKDANFESLRLIVLGGEKIIPT
                     DVIAFRKMYGHTEFINHYGPTEATIGAIAGRVDLSEPDAFAKRPTIGRPIANAGALVLN
                     EALKLVPPGASGQLYITGQGLARGYLNRPQLTAERFVENPYSPGSLMYKTGDVVRRLSD
                     GTLAFIGRADDQVKIRGYRIEPKEIETVMLSLSGIQEAVVLAVSEGGLQELCAYYTSDQ
                     AIEKAELRNQLSLTLPSHMIPAFFVQVDAIPLTANGKTDRNALPKPNAAQSGGKALAAP
                     ETALEESLCRIWQKTLGIEAIGIDDNFFDLGGHSLKGMMLIANIQAELEKSVPLKALFE
                     QPTVRQLAAYMEASAVSGGHQVLKPADKQDMYPLSSAQKRMYVLNQLDRQTISYNMPSV
                     LLMEGELDISCLRDSLNQLVNRHESLRTSFMEANGEPVQRIIEKAEVDLHVFEAKEDEA
                     DQKIKEFIRPFDLNDAPLIRAALLRIEAKKHLLLLDMHHIIADGVSRGIFVKELALLYK
                     GEQLPEPALHYKDFAVWQNEAEQQERMKEHEAYWMSVLSGELPELDLPLDYARPPVQSF
                     KGDTIRFRTGSETAKAVEKLLAETGTTLHMVLHAVFHVFLSKISGQRDIVIGSVTAGRT
                     NADVQDMPGMFVNTLALRMEAKEQQTFAELLEQAKQTNLSALEHQEYPFEDLVNQLDLP
                     RDMSRNPLFNVMVTTENPDKEQLTLQNLSISPYEAHQGTSKFDLTLGGFTDENGIGLQL
                     EYATDLFAKETAEKWSEYVLRLLKAVADNPNQPLSSLSLVTETEKQALLEAWKGKALPV
                     PTDKTVHQLFEETVQRHKDRPAVTYNGQSWTYGELNAKANRLARILMDCGISPDDRVGI
                     LTKPSLEMSAAVLGVLKAGAAFVPIDPDYPDQRIEYILQDSGAKLLLKQEGISVPDSFT
                     GDVILLDGSRTILSLPLDENDEGNPETAVTAENLAYMIYTSGTTGQPKG"
     source          1..10262
                     /collection_date="2010"
                     /db_xref="taxon:1423"
                     /geo_loc_name="USA: Florida"
                     /isolation_source="soil"
                     /lat_lon="27.26 N 80.20 W"
                     /mol_type="genomic DNA"
                     /organism="Bacillus subtilis"
                     /strain="PT26A"
                     /submitter_seqid="contig_22"
     region          1..10262
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..10262
                     /SMILES="NC(C(C)C)C(=O)NC(CC(=O)O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     CDS_motif       complement(3..47)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23850_0011"
                     /evalue="2.60E-06"
                     /label="NRPS-A_a3"
                     /locus_tag="CFI07_RS23850"
                     /protein_end="1215"
                     /protein_start="1200"
                     /score="20.0"
                     /tool="antismash"
                     /translation="AYMIYTSGTTGQPKG"
     CDS_motif       complement(249..290)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23850_0010"
                     /evalue="1.20E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="CFI07_RS23850"
                     /protein_end="1133"
                     /protein_start="1119"
                     /score="18.4"
                     /tool="antismash"
                     /translation="LGVLKAGAAFVPID"
     CDS_motif       complement(819..932)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23850_0009"
                     /evalue="7.10E-18"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="CFI07_RS23850"
                     /protein_end="943"
                     /protein_start="905"
                     /score="57.0"
                     /tool="antismash"
                     /translation="KQTNLSALEHQEYPFEDLVNQLDLPRDMSRNPLFNVMV"
     aSDomain        complement(972..1832)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CFI07_RS23850_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="3.60E-106"
                     /label="CFI07_RS23850_Condensation_LCL.1"
                     /locus_tag="CFI07_RS23850"
                     /protein_end="892"
                     /protein_start="605"
                     /score="346.5"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIADG"
                     /tool="antismash"
                     /translation="DMYPLSSAQKRMYVLNQLDRQTISYNMPSVLLMEGELDISCLRDS
                     LNQLVNRHESLRTSFMEANGEPVQRIIEKAEVDLHVFEAKEDEADQKIKEFIRPFDLND
                     APLIRAALLRIEAKKHLLLLDMHHIIADGVSRGIFVKELALLYKGEQLPEPALHYKDFA
                     VWQNEAEQQERMKEHEAYWMSVLSGELPELDLPLDYARPPVQSFKGDTIRFRTGSETAK
                     AVEKLLAETGTTLHMVLHAVFHVFLSKISGQRDIVIGSVTAGRTNADVQDMPGMFVNTL
                     ALRMEA"
     CDS_motif       complement(981..1070)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23850_0008"
                     /evalue="1.30E-14"
                     /label="C5_LCL_267-296"
                     /locus_tag="CFI07_RS23850"
                     /protein_end="889"
                     /protein_start="859"
                     /score="46.4"
                     /tool="antismash"
                     /translation="DIVIGSVTAGRTNADVQDMPGMFVNTLALR"
     CDS_motif       complement(1329..1361)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23850_0007"
                     /evalue="1.50E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="CFI07_RS23850"
                     /protein_end="773"
                     /protein_start="762"
                     /score="14.4"
                     /tool="antismash"
                     /translation="HYKDFAVWQNE"
     CDS_motif       complement(1392..1457)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23850_0006"
                     /evalue="2.20E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="CFI07_RS23850"
                     /protein_end="752"
                     /protein_start="730"
                     /score="29.8"
                     /tool="antismash"
                     /translation="MHHIIADGVSRGIFVKELALLY"
     CDS_motif       complement(1653..1763)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23850_0005"
                     /evalue="8.40E-16"
                     /label="C2_LCL_024-062"
                     /locus_tag="CFI07_RS23850"
                     /protein_end="665"
                     /protein_start="628"
                     /score="50.3"
                     /tool="antismash"
                     /translation="SYNMPSVLLMEGELDISCLRDSLNQLVNRHESLRTSF"
     aSDomain        complement(1881..2084)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CFI07_RS23850_PCP.1"
                     /evalue="9.60E-26"
                     /label="CFI07_RS23850_PCP.1"
                     /locus_tag="CFI07_RS23850"
                     /protein_end="589"
                     /protein_start="521"
                     /score="81.8"
                     /tool="antismash"
                     /translation="EESLCRIWQKTLGIEAIGIDDNFFDLGGHSLKGMMLIANIQAELE
                     KSVPLKALFEQPTVRQLAAYMEA"
     aSModule        complement(1881..4886)
                     /complete
                     /domains="nrpspksdomains_CFI07_RS23855_Condensation_LCL.1"
                     /domains="nrpspksdomains_CFI07_RS23850_AMP-binding.1"
                     /domains="nrpspksdomains_CFI07_RS23850_PCP.1"
                     /locus_tags="CFI07_RS23850"
                     /locus_tags="CFI07_RS23855"
                     /monomer_pairings="Asp -> Asp"
                     /monomer_pairings="Asp -> Asp"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(2373..2438)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23850_0004"
                     /evalue="4.20E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="CFI07_RS23850"
                     /protein_end="425"
                     /protein_start="403"
                     /score="37.9"
                     /tool="antismash"
                     /translation="FIGRADDQVKIRGYRIEPKEIE"
     aSDomain        complement(2403..3611)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CFI07_RS23850_AMP-binding.1"
                     /evalue="9.00E-116"
                     /label="CFI07_RS23850_AMP-binding.1"
                     /locus_tag="CFI07_RS23850"
                     /protein_end="415"
                     /protein_start="12"
                     /score="378.8"
                     /specificity="substrate consensus: Asp"
                     /tool="antismash"
                     /translation="FEKQAQQTPDHPAVKAGGNLLTYRELDEQANQLAHHLRAQGAGNE
                     DIVAIVMDRSAEVMVSILGVMKAGAAFLPIDPDTPEERIRYSLEDSGAKFAVVNERNMT
                     AIGQYEGTIVSLDDGKWRNESKECPSSISGSRNLAYVIYTSGTTGKPKGVQIEHRNLTN
                     YVSWFSEEAGLTENDKTVLLSSYAFDLGYTSMFPVLLGGGELHIVQKETYTAPDEIAHY
                     IKEHGITYIKLTPSLFHTIVNTASFAKDANFESLRLIVLGGEKIIPTDVIAFRKMYGHT
                     EFINHYGPTEATIGAIAGRVDLSEPDAFAKRPTIGRPIANAGALVLNEALKLVPPGASG
                     QLYITGQGLARGYLNRPQLTAERFVENPYSPGSLMYKTGDVVRRLSDGTLAFIGRADDQ
                     VKIR"
     CDS_motif       complement(2520..2609)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23850_0003"
                     /evalue="5.30E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="CFI07_RS23850"
                     /protein_end="376"
                     /protein_start="346"
                     /score="50.7"
                     /tool="antismash"
                     /translation="PPGASGQLYITGQGLARGYLNRPQLTAERF"
     CDS_motif       complement(3138..3197)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23850_0002"
                     /evalue="1.20E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="CFI07_RS23850"
                     /protein_end="170"
                     /protein_start="150"
                     /score="33.2"
                     /tool="antismash"
                     /translation="AYVIYTSGTTGKPKGVQIEH"
     CDS_motif       complement(3384..3425)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23850_0001"
                     /evalue="4.20E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="CFI07_RS23850"
                     /protein_end="88"
                     /protein_start="74"
                     /score="16.7"
                     /tool="antismash"
                     /translation="LGVMKAGAAFLPID"
     gene            complement(<3720..>4892)
                     /locus_tag="CFI07_RS23855"
                     /pseudo=""
     CDS             complement(<3720..4892)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (2-264). E-value:
                     3.8e-94. Score: 307.0. Matches aSDomain:
                     nrpspksdomains_CFI07_RS23855_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="CFI07_RS23855"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="condensation domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 2.4e-85, bitscore:
                     274.5, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MANTSYNVPAVLLLEGEVDKDRLENAIQQLINRHEILRTSFDMID
                     GEVVQTVHKNISFQLEAAKGREEDAEEIIKAFVQPFELNCAPLVRSKLVQLGEKRHLLL
                     IDMHHIITDGSSTGILIGDLAKIYQGADLELPQIHYKDYAVWHKEQTNYQKDEEYWLDV
                     FKGELPILDLPADFERPAERSFAGERVMFGLDKQITAQIKSLMAETDTTMYMFLLAAFN
                     VLLSKYASQDDIIVGSPTAGRTHPDLQGVPGMFVNTVALRTAPAGDKTFAQFLEEVKTA
                     SLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVMFNMQNMEIPSLRLGDLKISSYSMLH
                     HVAKFDLSLEAVEREEDIGLSFDYATALFKDETIRRWSRHFVNIIKAAAAN"
     CDS_motif       complement(3828..3875)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23855_0006"
                     /evalue="1.00E+01"
                     /label="C3_dual_135-156"
                     /locus_tag="CFI07_RS23855"
                     /protein_end="355"
                     /protein_start="339"
                     /score="-0.4"
                     /tool="antismash"
                     /translation="HHVAKFDLSLEAVERE"
     CDS_motif       complement(3939..4052)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23855_0005"
                     /evalue="7.60E-17"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="CFI07_RS23855"
                     /protein_end="318"
                     /protein_start="280"
                     /score="53.6"
                     /tool="antismash"
                     /translation="ASLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVMFNM"
     aSDomain        complement(4101..4886)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CFI07_RS23855_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="3.80E-94"
                     /label="CFI07_RS23855_Condensation_LCL.1"
                     /locus_tag="CFI07_RS23855"
                     /protein_end="264"
                     /protein_start="2"
                     /score="307.0"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIITDG"
                     /tool="antismash"
                     /translation="NTSYNVPAVLLLEGEVDKDRLENAIQQLINRHEILRTSFDMIDGE
                     VVQTVHKNISFQLEAAKGREEDAEEIIKAFVQPFELNCAPLVRSKLVQLGEKRHLLLID
                     MHHIITDGSSTGILIGDLAKIYQGADLELPQIHYKDYAVWHKEQTNYQKDEEYWLDVFK
                     GELPILDLPADFERPAERSFAGERVMFGLDKQITAQIKSLMAETDTTMYMFLLAAFNVL
                     LSKYASQDDIIVGSPTAGRTHPDLQGVPGMFVNTVALRTA"
     CDS_motif       complement(4107..4196)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23855_0004"
                     /evalue="3.00E-16"
                     /label="C5_LCL_267-296"
                     /locus_tag="CFI07_RS23855"
                     /protein_end="262"
                     /protein_start="232"
                     /score="51.7"
                     /tool="antismash"
                     /translation="DIIVGSPTAGRTHPDLQGVPGMFVNTVALR"
     CDS_motif       complement(4443..4478)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23855_0003"
                     /evalue="4.10E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="CFI07_RS23855"
                     /protein_end="150"
                     /protein_start="138"
                     /score="13.1"
                     /tool="antismash"
                     /translation="HYKDYAVWHKEQ"
     CDS_motif       complement(4509..4574)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23855_0002"
                     /evalue="2.80E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="CFI07_RS23855"
                     /protein_end="128"
                     /protein_start="106"
                     /score="29.5"
                     /tool="antismash"
                     /translation="MHHIITDGSSTGILIGDLAKIY"
     CDS_motif       complement(4770..4883)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23855_0001"
                     /evalue="5.20E-18"
                     /label="C2_LCL_024-062"
                     /locus_tag="CFI07_RS23855"
                     /protein_end="41"
                     /protein_start="3"
                     /score="57.4"
                     /tool="antismash"
                     /translation="TSYNVPAVLLLEGEVDKDRLENAIQQLINRHEILRTSF"
     gene            complement(4838..4978)
                     /gene="comS"
                     /locus_tag="CFI07_RS23860"
     CDS             complement(4838..4978)
                     /codon_start=1
                     /gene="comS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388232.1"
                     /locus_tag="CFI07_RS23860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="competence protein ComS"
                     /protein_id="WP_010886404.1"
                     /transl_table=11
                     /translation="MNRSGKHLISSIILYPRPSGECISSISLDKQTQATTSPLYFCWRE
                     K"
     gene            complement(<5070..8114)
                     /gene="srfAB"
                     /locus_tag="CFI07_RS23865"
                     /pseudo=""
     CDS             complement(<5070..8114)
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (0-29). E-value: 1.4e-14.
                     Score: 45.0. Matches aSDomain:
                     nrpspksdomains_CFI07_RS23865_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_DCL (6-305). E-value:
                     7.2e-115. Score: 375.1. Matches aSDomain:
                     nrpspksdomains_CFI07_RS23865_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (470-864). E-value:
                     3.2e-112. Score: 367.0. Matches aSDomain:
                     nrpspksdomains_CFI07_RS23865_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (970-1013). E-value: 3.3e-14. Score:
                     44.8. Matches aSDomain: nrpspksdomains_CFI07_RS23865_PCP.1"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="CFI07_RS23865"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 4.6e-92, bitscore:
                     296.6, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 4.8e-117, bitscore:
                     379.3, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 4.8e-09, bitscore:
                     25.2, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKKSIQKVYALTPMQEGMLYHAMLDPHSSSYFTQLELGIHGAFD
                     LEIFEKSVNELIRSYDILRTVFVHQQLQKPRQVVLAERKTKVHYEDISHADENRQKEHI
                     ERYKQDVQRQGFNLAKDILFKVAVFRLAADQLYLVWSNHHIMMDGWSMGVLMKSLFQNY
                     EALRAGRTPANGQGKPYSDYIKWLGKQDNEEAESYWSERLAGFEQPSVLPGRLPVKKDE
                     YVNKEYSFTWDETLVARIQQTANLHQVTGPNLFQAVWGIVLSKYNFTDDVIFGTVVSGR
                     PSEINGIETMAGLFINTIPVRVKVERDAAFADIFTAVQQHAVEAERYDYVPLYEIQKRS
                     ALDGNLLNHLVAFENYPLDQELENGSMEDRLGFSIKVESAFEQTSFDFNLIVYPGKTWT
                     VKIKYNGAAFDSAFIERTAEHLTRMMEAAVAQPAAFVREYGLVGNEEQRQIVEVFNSTK
                     AELPEGMAVHQVFEEQAKRTPASTAVVYEGTELTYRELNATANRLARKLVEQGLQKGET
                     AAIMNDRSVETVVGMLAVLKAGAAYVPLDPALPGARLRFMAEDSSVRMVLTGNSYTGQA
                     HQLQVPVLTLDIGFEESEAADNLNLPSAPSDLAYIMYTSGSTGKPKGVMIEHKSILRLV
                     KNAGYVPVTEEDRMAQTGAVSFDAGTFEVFGALLNGAALYPVKKETLLDAKQFAAFLRE
                     QSITTMWLTSPLFNQLAAKDAGMFGTLRHLIIGGDALVPHIVSKVKQASPSLSLWNGYG
                     PTENTTFSTSFLIDREYGGSIPIGKPIGNSTAYILDEQQCLQPIGAPGELCVGGIGVAR
                     GYVNLPELTEKQFLEDPFRPGERIYRTGDLARWLPDGNIEFLGRIDNQVKVRGFRIELG
                     EIETKLNMAEHVTEAAVIIRKNKADENEICAYFTADREVAVIELRKTLSQSLPDYMVPA
                     HLIQMDSLPLTPNGKINKKELPAPQSEAVQPEYAAPKTESEKKLAEIWEGILGVKAGVT
                     DNFFMIGGHSLKAMMMTAKIQEHFHK"
     aSDomain        complement(5076..5204)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CFI07_RS23865_PCP.1"
                     /evalue="3.30E-14"
                     /label="CFI07_RS23865_PCP.1"
                     /locus_tag="CFI07_RS23865"
                     /protein_end="1013"
                     /protein_start="970"
                     /score="44.8"
                     /tool="antismash"
                     /translation="EKKLAEIWEGILGVKAGVTDNFFMIGGHSLKAMMMTAKIQEHF"
     aSModule        complement(5076..8096)
                     /complete
                     /domains="nrpspksdomains_CFI07_RS23865_Condensation_DCL.1"
                     /domains="nrpspksdomains_CFI07_RS23865_AMP-binding.1"
                     /domains="nrpspksdomains_CFI07_RS23865_PCP.1"
                     /locus_tags="CFI07_RS23865"
                     /monomer_pairings="Val -> Val"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(5493..5558)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23865_0010"
                     /evalue="1.40E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="CFI07_RS23865"
                     /protein_end="874"
                     /protein_start="852"
                     /score="39.4"
                     /tool="antismash"
                     /translation="FLGRIDNQVKVRGFRIELGEIE"
     aSDomain        complement(5523..6704)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CFI07_RS23865_AMP-binding.1"
                     /evalue="3.20E-112"
                     /label="CFI07_RS23865_AMP-binding.1"
                     /locus_tag="CFI07_RS23865"
                     /protein_end="864"
                     /protein_start="470"
                     /score="367.0"
                     /specificity="substrate consensus: Val"
                     /tool="antismash"
                     /translation="FEEQAKRTPASTAVVYEGTELTYRELNATANRLARKLVEQGLQKG
                     ETAAIMNDRSVETVVGMLAVLKAGAAYVPLDPALPGARLRFMAEDSSVRMVLTGNSYTG
                     QAHQLQVPVLTLDIGFEESEAADNLNLPSAPSDLAYIMYTSGSTGKPKGVMIEHKSILR
                     LVKNAGYVPVTEEDRMAQTGAVSFDAGTFEVFGALLNGAALYPVKKETLLDAKQFAAFL
                     REQSITTMWLTSPLFNQLAAKDAGMFGTLRHLIIGGDALVPHIVSKVKQASPSLSLWNG
                     YGPTENTTFSTSFLIDREYGGSIPIGKPIGNSTAYILDEQQCLQPIGAPGELCVGGIGV
                     ARGYVNLPELTEKQFLEDPFRPGERIYRTGDLARWLPDGNIEFLGRIDNQVKVR"
     CDS_motif       complement(5640..5729)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23865_0009"
                     /evalue="2.70E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="CFI07_RS23865"
                     /protein_end="825"
                     /protein_start="795"
                     /score="48.4"
                     /tool="antismash"
                     /translation="PIGAPGELCVGGIGVARGYVNLPELTEKQF"
     CDS_motif       complement(6231..6290)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23865_0008"
                     /evalue="1.80E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="CFI07_RS23865"
                     /protein_end="628"
                     /protein_start="608"
                     /score="35.6"
                     /tool="antismash"
                     /translation="AYIMYTSGSTGKPKGVMIEH"
     CDS_motif       complement(6477..6518)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23865_0007"
                     /evalue="1.50E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="CFI07_RS23865"
                     /protein_end="546"
                     /protein_start="532"
                     /score="21.1"
                     /tool="antismash"
                     /translation="LAVLKAGAAYVPLD"
     CDS_motif       complement(7044..7160)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23865_0006"
                     /evalue="1.70E-17"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="CFI07_RS23865"
                     /protein_end="357"
                     /protein_start="318"
                     /score="55.8"
                     /tool="antismash"
                     /translation="QQHAVEAERYDYVPLYEIQKRSALDGNLLNHLVAFENYP"
     aSDomain        complement(7200..8096)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CFI07_RS23865_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="7.20E-115"
                     /label="CFI07_RS23865_Condensation_DCL.1"
                     /locus_tag="CFI07_RS23865"
                     /protein_end="305"
                     /protein_start="6"
                     /score="375.1"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIMMDG"
                     /tool="antismash"
                     /translation="QKVYALTPMQEGMLYHAMLDPHSSSYFTQLELGIHGAFDLEIFEK
                     SVNELIRSYDILRTVFVHQQLQKPRQVVLAERKTKVHYEDISHADENRQKEHIERYKQD
                     VQRQGFNLAKDILFKVAVFRLAADQLYLVWSNHHIMMDGWSMGVLMKSLFQNYEALRAG
                     RTPANGQGKPYSDYIKWLGKQDNEEAESYWSERLAGFEQPSVLPGRLPVKKDEYVNKEY
                     SFTWDETLVARIQQTANLHQVTGPNLFQAVWGIVLSKYNFTDDVIFGTVVSGRPSEING
                     IETMAGLFINTIPVRVKV"
     CDS_motif       complement(7209..7304)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23865_0005"
                     /evalue="2.30E-19"
                     /label="C5_DCL_263-294"
                     /locus_tag="CFI07_RS23865"
                     /protein_end="302"
                     /protein_start="270"
                     /score="61.8"
                     /tool="antismash"
                     /translation="DVIFGTVVSGRPSEINGIETMAGLFINTIPVR"
     CDS_motif       complement(7542..7580)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23865_0004"
                     /evalue="1.20E-03"
                     /label="C4_DCL_171-183"
                     /locus_tag="CFI07_RS23865"
                     /protein_end="191"
                     /protein_start="178"
                     /score="11.5"
                     /tool="antismash"
                     /translation="PYSDYIKWLGKQD"
     CDS_motif       complement(7626..7691)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23865_0003"
                     /evalue="1.10E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="CFI07_RS23865"
                     /protein_end="163"
                     /protein_start="141"
                     /score="37.0"
                     /tool="antismash"
                     /translation="NHHIMMDGWSMGVLMKSLFQNY"
     CDS_motif       complement(7908..8024)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23865_0002"
                     /evalue="2.80E-14"
                     /label="C2_DCL_024-062"
                     /locus_tag="CFI07_RS23865"
                     /protein_end="69"
                     /protein_start="30"
                     /score="45.3"
                     /tool="antismash"
                     /translation="SYFTQLELGIHGAFDLEIFEKSVNELIRSYDILRTVFVH"
     aSDomain        complement(8028..8114)
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CFI07_RS23865_NRPS-COM_Nterm.1"
                     /evalue="1.40E-14"
                     /label="CFI07_RS23865_NRPS-COM_Nterm.1"
                     /locus_tag="CFI07_RS23865"
                     /protein_end="29"
                     /protein_start="0"
                     /score="45.0"
                     /tool="antismash"
                     /translation="MSKKSIQKVYALTPMQEGMLYHAMLDPHS"
     CDS_motif       complement(8049..8087)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23865_0001"
                     /evalue="2.30E-05"
                     /label="C1_dual_004-017"
                     /locus_tag="CFI07_RS23865"
                     /protein_end="22"
                     /protein_start="9"
                     /score="17.0"
                     /tool="antismash"
                     /translation="YALTPMQEGMLYH"
     gene            complement(8127..>10262)
                     /locus_tag="CFI07_RS23020"
     CDS             complement(8127..>10262)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (167-235). E-value: 5e-28. Score:
                     89.1. Matches aSDomain: nrpspksdomains_CFI07_RS23020_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (245-548). E-value:
                     5.2e-179. Score: 588.6. Matches aSDomain:
                     nrpspksdomains_CFI07_RS23020_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (553-707). E-value: 8.1e-56.
                     Score: 180.1. Matches aSDomain:
                     nrpspksdomains_CFI07_RS23020_TIGR01720.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388230.2"
                     /locus_tag="CFI07_RS23020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="condensation domain-containing protein"
                     /protein_id="WP_162869004.1"
                     /sec_met_domain="AMP-binding (E-value: 2e-33, bitscore:
                     103.9, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 4.6e-19, bitscore:
                     57.3, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.1e-98, bitscore:
                     318.4, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="ELCVAGRGLARGYLNREDETAKRFVADPFVPGERMYRTGDLVKWT
                     GGGIEYIGRIDQQVKVRGYRIELSEIEVQLAQLSEVQDAAVTAVKDKGGNTAIAAYVTP
                     ETADIEALKSALKETLPDYMIPAFWVTLNELPVTANGKVDRKALPEPDIEAGSGEYKAP
                     TTDMEELLAGIWQDVLGMSEVGVTDNFFSLGGDSIKGIQMASRLNQHGWRLEMKDLFQH
                     PTIEELTQYVERAEGKQADQGPVEGEVILTPIQRWFFEKNFTNKHHWNQSVMLHAKKGF
                     DPERVEKTLQALIEHHDALRMVYREGQEDVIQYNRGLEAASAQLEVIQIEGQAADYEDR
                     IEREAERLQSSIDLQEGGLLKAGLFQAEDGDHLLLAIHHLVVDGVSWRILLEDFAAVYT
                     QLEQGNEPVLPQKTHSFAEYAERLQDFANSKAFLKEKEYWRQLEEQAVAAKLPKDRESG
                     DQRMKHTKTIEFSLTAEETEQLTTKVHEAYHTEMNDILLTAFGLAMKEWTGQDRVNVHL
                     EGHGREEIIEDLTISRTVGWFTSMYPMVLDMKHADDLGYQLKQMKEDIRHVPNKGVGYG
                     ILRYLTASEHKEDVAFSIQPDVSFNYLGQFDEMSDAGLFTRSELPSGQSLSPETEKPNA
                     LDVVGYIENGKLTMSLAYHSLEFHEKTVQTFSDSFKAHLLRIIEHCLSQDGTELTPSDL
                     GDDDLTLDELDKLMEIF"
     aSDomain        complement(8139..8600)
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CFI07_RS23020_TIGR01720.1"
                     /evalue="8.10E-56"
                     /label="CFI07_RS23020_TIGR01720.1"
                     /locus_tag="CFI07_RS23020"
                     /protein_end="707"
                     /protein_start="553"
                     /score="180.1"
                     /tool="antismash"
                     /translation="LGYQLKQMKEDIRHVPNKGVGYGILRYLTASEHKEDVAFSIQPDV
                     SFNYLGQFDEMSDAGLFTRSELPSGQSLSPETEKPNALDVVGYIENGKLTMSLAYHSLE
                     FHEKTVQTFSDSFKAHLLRIIEHCLSQDGTELTPSDLGDDDLTLDELDKL"
     aSModule        complement(8139..9758)
                     /domains="nrpspksdomains_CFI07_RS23020_PCP.1"
                     /domains="nrpspksdomains_CFI07_RS23020_Epimerization.1"
                     /domains="nrpspksdomains_CFI07_RS23020_TIGR01720.1"
                     /incomplete
                     /locus_tags="CFI07_RS23020"
                     /tool="antismash"
                     /type="unknown"
     CDS_motif       complement(8517..8576)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23020_0007"
                     /evalue="1.30E-09"
                     /label="NRPS-E6"
                     /locus_tag="CFI07_RS23020"
                     /protein_end="581"
                     /protein_start="561"
                     /score="30.3"
                     /tool="antismash"
                     /translation="KEDIRHVPNKGVGYGILRYL"
     aSDomain        complement(8616..9524)
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CFI07_RS23020_Epimerization.1"
                     /evalue="5.20E-179"
                     /label="CFI07_RS23020_Epimerization.1"
                     /locus_tag="CFI07_RS23020"
                     /protein_end="548"
                     /protein_start="245"
                     /score="588.6"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="EGEVILTPIQRWFFEKNFTNKHHWNQSVMLHAKKGFDPERVEKTL
                     QALIEHHDALRMVYREGQEDVIQYNRGLEAASAQLEVIQIEGQAADYEDRIEREAERLQ
                     SSIDLQEGGLLKAGLFQAEDGDHLLLAIHHLVVDGVSWRILLEDFAAVYTQLEQGNEPV
                     LPQKTHSFAEYAERLQDFANSKAFLKEKEYWRQLEEQAVAAKLPKDRESGDQRMKHTKT
                     IEFSLTAEETEQLTTKVHEAYHTEMNDILLTAFGLAMKEWTGQDRVNVHLEGHGREEII
                     EDLTISRTVGWFTSMYPMVLDM"
     CDS_motif       complement(8622..8669)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23020_0006"
                     /evalue="1.30E-08"
                     /label="NRPS-E5"
                     /locus_tag="CFI07_RS23020"
                     /protein_end="546"
                     /protein_start="530"
                     /score="26.8"
                     /tool="antismash"
                     /translation="ISRTVGWFTSMYPMVL"
     CDS_motif       complement(9078..9143)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23020_0005"
                     /evalue="2.40E-12"
                     /label="NRPS-E2"
                     /locus_tag="CFI07_RS23020"
                     /protein_end="394"
                     /protein_start="372"
                     /score="38.9"
                     /tool="antismash"
                     /translation="LLLAIHHLVVDGVSWRILLEDF"
     CDS_motif       complement(9345..9419)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23020_0004"
                     /evalue="2.60E-06"
                     /label="C2_LCL_024-062"
                     /locus_tag="CFI07_RS23020"
                     /protein_end="305"
                     /protein_start="280"
                     /score="19.8"
                     /tool="antismash"
                     /translation="FDPERVEKTLQALIEHHDALRMVYR"
     CDS_motif       complement(9480..9509)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23020_0003"
                     /evalue="4.50E-05"
                     /label="NRPS-E1"
                     /locus_tag="CFI07_RS23020"
                     /protein_end="260"
                     /protein_start="250"
                     /score="16.1"
                     /tool="antismash"
                     /translation="LTPIQRWFFE"
     aSDomain        complement(9555..9758)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CFI07_RS23020_PCP.1"
                     /evalue="5.00E-28"
                     /label="CFI07_RS23020_PCP.1"
                     /locus_tag="CFI07_RS23020"
                     /protein_end="235"
                     /protein_start="167"
                     /score="89.1"
                     /tool="antismash"
                     /translation="EELLAGIWQDVLGMSEVGVTDNFFSLGGDSIKGIQMASRLNQHGW
                     RLEMKDLFQHPTIEELTQYVERA"
     CDS_motif       complement(10044..10109)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23020_0002"
                     /evalue="1.40E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="CFI07_RS23020"
                     /protein_end="72"
                     /protein_start="50"
                     /score="36.3"
                     /tool="antismash"
                     /translation="YIGRIDQQVKVRGYRIELSEIE"
     CDS_motif       complement(10188..10259)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CFI07_RS23020_0001"
                     /evalue="4.20E-12"
                     /label="NRPS-A_a6"
                     /locus_tag="CFI07_RS23020"
                     /protein_end="24"
                     /protein_start="0"
                     /score="38.3"
                     /tool="antismash"
                     /translation="ELCVAGRGLARGYLNREDETAKRF"
ORIGIN
        1 acacccttcg gctgtccggt cgttccagac gtgtaaatca tatacgccaa gttctccgcg
       61 gttacagcgg tttctggatt tccctcgtcg ttttcatcaa gcggcaggct tagaatcgtg
      121 cggcttccgt cgagaagaat gacatctccc gtaaagctgt ccggcactga aatgccttcc
      181 tgtttcaaga gaagcttcgc gccgctgtcc tgtaaaatat actcaatccg ctgatccggg
      241 tagtcaggat caatcggcac aaacgccgct ccggctttca agacgccgag caccgcggcg
      301 gacatttcaa gcgacggctt cgtgagaatg ccgacgcggt catccgggct gatgccgcag
      361 tccatcagaa tccgcgcgag acggtttgcc ttcgcattca gctcgccgta cgtccaagac
      421 tggccgttgt atgtgacagc agggcggtct ttgtggcgct ggacagtctc ttcgaatagc
      481 tgatgaaccg ttttgtctgt cggcacaggc agcgccttgc ccttccatgc ttcaagaagc
      541 gcttgctttt ctgtctcggt gaccagtgac aggctggaaa gcggctggtt cgggttatcc
      601 gcaacagcct ttagtagccg cagaacgtat tcgctccatt tttcagctgt ttcttttgcg
      661 aacagatctg tcgcatattc gagctgcaag ccaatgccat tttcatcagt aaatccgccc
      721 agtgtcagat caaacttaga agttccctga tgagcctcat aaggtgaaat gctcagattt
      781 tgcaatgtaa gctgttcttt atcagggttt tctgtcgtca ccatcacatt aaacaatggg
      841 tttcggctca tatcccgagg gagatcaagc tgattaacca gatcttcaaa cggatactcc
      901 tgatgctcaa gcgctgacag gttcgtctgc tttgcttgct ccaaaagctc cgcaaatgtt
      961 tgctgttctt tcgcttccat tctcagagca agtgtattga cgaacattcc cggcatgtcc
     1021 tgaacatcag cattcgtccg gccggcagta acggagccga tgacgatatc ccgctgtccg
     1081 gaaattttgc ttaaaaagac gtggaaaaca gcatgaagca ccatgtgcaa ggtcgttccg
     1141 gtttccgcaa gcagtttttc taccgccttt gccgtctcac ttcccgtacg gaaacggatc
     1201 gtatctcctt taaagctttg cacaggcgga cgggcgtaat cgagcgggag atcaagctct
     1261 ggcagctcgc ctgaaagaac tgacatccag tacgcctcat gctccttcat ccgttcttgt
     1321 tgctcagctt cattttgcca aacggcgaaa tctttataat gaagcgccgg ctccggaagc
     1381 tgctctcctt tgtaaagcag cgccaattct tttacaaaga tgcctcttga gacgccgtct
     1441 gcgatgatat gatgcatatc taaaagcagc aaatgttttt tcgcttctat tcgaagcaaa
     1501 gctgcgcgaa tgagcggtgc gtcgtttaag tcgaatggcc ggataaattc cttaatcttt
     1561 tggtccgctt cgtcttcttt ggcttcaaac acatgaagat caacctccgc cttctcaatg
     1621 atgcgctgaa caggctcacc attagcttcc ataaatgacg tccgcaatga ttcgtgacgg
     1681 ttcacaagct gattgagtga atcgcgcagg cacgaaatat caagctctcc ttccattaga
     1741 agaacagatg gcatgttgta gcttatcgtc tggcggtcaa gctgattgag cacgtacatc
     1801 cgtttctgtg cggatgacaa tggatacata tcctgcttgt cagccggttt gaggacttga
     1861 tggccgcctg aaacagcaga cgcctccata taagccgcca gctggcgaac agtcggctgc
     1921 tcgaacagtg ctttaagcgg tacgcttttc tccaattccg cctgaatgtt ggcaatcagc
     1981 atcatccctt ttaatgaatg gccgcctaaa tcgaaaaaat tgtcgtcaat gccgatggct
     2041 tctatgccaa gcgttttctg ccaaatgcgg cataagcttt cttcaagcgc tgtctccggt
     2101 gcggccaagg ctttgccacc ggattgtgcc gcgttcggct tcggcaaagc gtttctgtcg
     2161 gtttttccgt ttgccgtcag cggaatcgcg tcaacctgca caaagaaagc aggaatcata
     2221 tgagacggca gtgttaggga aagctggttc cggagctctg ctttttcaat agcttgatcc
     2281 gacgtataat acgcgcaaag ctcttgaaga ccgccctcgg aaaccgctag tacaaccgct
     2341 tcttgaattc cgctgaggct gagcatgacc gtttcaattt ctttcggctc aatgcggtag
     2401 ccgcggattt tcacctgatc atcagcccgg ccgataaatg caagcgtacc gtcagaaagt
     2461 ctccgtacga catctccggt tttgtacatg aggcttcccg gcgaatatgg attttctaca
     2521 aatctctcgg ctgtcagctg aggcctgttg agataccctc tcgcaagccc ctgtcccgtg
     2581 atatagagct gtccgctcgc tcccggcggc acaagcttca atgcttcatt taagacaagc
     2641 gcgccggcat tcgcaatcgg ccgtccgatt gtcgggcgtt tcgcaaatgc atccggttca
     2701 gacagatcaa cccgcccggc gatggcgccg atcgttgctt ctgtcgggcc gtagtgattg
     2761 ataaattcgg tatgtccata catcttacgg aaggcgataa catcagtcgg gatgattttt
     2821 tctcctccca agacgatcaa gcgcaaggat tcaaagttcg catcttttgc aaaactggcg
     2881 gtgttcacta ttgtatggaa cagagacggt gtcagcttaa tataagtgat cccatgctcc
     2941 ttgatatagt gcgctatttc atccggcgcc gtataggttt ccttctggac gatatggagc
     3001 tcgcccccgc ccagaagtac agggaacatg ctcgtatagc caaggtcaaa tgcgtaagat
     3061 gaaagcaata cagtcttatc attttccgtc aggcccgcct cttcactaaa ccaagagaca
     3121 tagtttgtca gattgcgatg ctcaatctgc acgccctttg gctttccggt cgtaccggac
     3181 gtataaatga cgtatgcaag attgcgagac ccggaaatgg atgatgggca ctccttgctt
     3241 tcatttctcc atttaccgtc atcaaggctg acgattgtcc cttcatattg cccaatagcc
     3301 gtcatgtttc tttcattcac gaccgcaaat tttgctccgc tgtcctctaa tgaataacgg
     3361 attcgttctt caggtgtatc aggatcaatc ggaaggaaag ctgcccccgc cttcatgaca
     3421 ccgagaatgg ataccatgac ttcagctgac cggtccataa caatcgcgac aatgtcttca
     3481 tttcctgccc cttgggcacg aagatgatgc gccagctggt tcgcctgttc atcaagctcg
     3541 cgataggtca gcagatttcc gccagctttc accgcaggat ggtcaggtgt ttgctgggcc
     3601 tgtttttcaa aaagagcggc aaaggttgct tcggtaattt gagtcatatg tctttcttct
     3661 agcaaagcag ccgtttctgc agatgaaagc agatctacat cagacagccg aacgtttgga
     3721 ttagccgcgg ccgctttgat gatattgaca aagtggcggc tccagcggcg gatcgtctcg
     3781 tccttaaaca aggcagtcgc atagtcaaag cttaggccga tatcctcttc acgctcgacc
     3841 gcttccaagg aaagatcaaa tttcgcaaca tgatgaagca tggaatacga ggaaatcttc
     3901 aaatctccta atcttaatga aggaatctcc atattctgca tgttgaacat cacgctgaac
     3961 agcggacttc tgcttgtatc ccttgtaagc ggaagctttt caatcaactc ctcaagcggg
     4021 tagctctggt gctcgaatgc ttgaaggcta gctgttttga cctcttcaag aaattgcgcg
     4081 aaggttttat ctcccgctgg tgccgttctg agtgccaccg tgttgacaaa catacccggc
     4141 acaccttgca gatcaggatg tgttctgcca gctgtcggcg agccaacaat gatatcatcc
     4201 tgtgacgcgt acttggaaag gagtacattg aacgccgcca gcaaaaacat gtacatcgtc
     4261 gtatctgttt ctgccatgag cgatttgatt tgagccgtga tttgcttatc aagcccaaac
     4321 atcacgcgct ctcccgcaaa gctccgttca gctggccgct cgaaatccgc gggaagatcc
     4381 agtattggca gttcgccttt aaagacatcg agccagtatt cctcatcttt ttgataatta
     4441 gtttgttctt tgtgccaaac tgcgtaatct ttatagtgaa tttgtggcag ttccagatca
     4501 gcgccttgat atattttggc aagatcaccg attagaatgc ctgttgaact gccgtcagta
     4561 ataatatgat gcatatcaat gagcagtagg tggcgttttt ctcccagctg gacaagcttc
     4621 gaacggacga gcggcgcgca gtttaattca aacggctgaa caaatgcttt gattatctct
     4681 tccgcgtctt cttcccgtcc cttggcagcc tccagctgga acgatatgtt tttatgaacg
     4741 gtttgcacaa cctctccgtc gatcatgtca aacgatgtac ggaggatttc gtgccggttg
     4801 attaattgct gaatcgcgtt ttcaagccgg tctttatcta cttctccctc cagcagaagt
     4861 acagcgggga cgttgtagct tgtgtttgct tgtccaagct gattgaggat atacattctc
     4921 cgctgggccg gggatacagg ataatgctgc tgataagatg cttgcctgat cggttcaaac
     4981 gtctgctcct ccttgctttc gttctcttcc aaatacagtg ccagttcttg aatagtcggc
     5041 ttttcaaaaa gcacttttat cggaacttcc ttatgaaaat gctcttgaat tttcgccgtc
     5101 atcatcatcg ctttcaaaga atggccgccg atcataaaga agttatcggt aacgcctgct
     5161 ttgacgccga gtattccttc ccagatctcc gctaatttct tttcactctc tgtttttggt
     5221 gctgcgtatt ccggctgcac ggcttctgat tgcggtgcag gcagttcctt tttgttgatt
     5281 ttcccgtttg gcgtgagcgg cagactgtcc atttgaatca ggtgggcagg gaccatatag
     5341 tcaggcaaag actgagacag tgtttttctc agctcgatca cagccacttc acggtccgcc
     5401 gtaaagtacg cgcaaatttc attttcatcc gctttgttct tgcggatgat caccgcggct
     5461 tctgtcacat gctcagccat gttcagtttt gtttcaattt cgccaagctc aattcggaag
     5521 ccgcgcacct tcacttggtt gtcgattctg cctaaaaatt cgatattgcc gtccggcagc
     5581 catcttgcca agtcaccagt gcgataaatt ctctcacccg gtctgaacgg atcttcgaga
     5641 aattgcttct ctgtcagctc agggagattg acatacccac gcgctacacc gattccgcct
     5701 acgcaaagct cgccaggcgc gccaattggc tgcaggcatt gctgctcatc caggatgtag
     5761 gcagtggaat ttccgatcgg cttcccgatt gggatagagc cgccatactc gcggtcgatc
     5821 agaaaacttg ttgaaaacgt cgtgttttct gtcgggccgt atccgttcca caatgaaagc
     5881 gacggcgatg cctgttttac tttgctgaca atatgcggga caagggcatc tccgccgatg
     5941 attaaatggc gcagtgtgcc gaacataccc gcatcctttg cagcaagctg gttgaataaa
     6001 ggtgatgtca gccacatagt tgtgatgctt tgctcacgca ggaatgcagc aaattgtttc
     6061 gcatcaagca gtgtctcttt tttgaccgga taaagcgctg cgccatttag cagtgcgccg
     6121 aacacttcaa acgttccggc atcaaagctg actgcccctg tttgcgccat gcggtcctct
     6181 tcagtaacag gaacgtaccc ggcatttttg acgaggcgca gaatgctttt atgttcgatc
     6241 atgacgcctt tcggtttgcc cgttgaaccg gatgtgtaca tgatgtacgc caaatcagac
     6301 ggggcggatg gcaggttgag attgtcagcc gcttcgctct cttcaaagcc tatgtccagt
     6361 gtaagaaccg gcacttgcag ctgatgtgcc tgccctgtat aagaatttcc ggtcaaaacc
     6421 attcgaacgg agctgtcctc tgccatgaaa cgaagacgag ccccaggaag cgctggatca
     6481 agcggcacat atgcggcacc tgcttttaac acagccagca tgccgacaac agtttctact
     6541 gagcgatcgt tcataatcgc tgctgtttcc cctttttgaa gcccttgttc gacaagcttt
     6601 ctcgccagac ggttagccgt tgcattcagc tcgcggtacg tcagctcggt tccttcatat
     6661 acgacggcgg tgctcgccgg cgtccgtttc gcttgctctt caaacacttg gtgtacagcc
     6721 attccttcag ggagttcggc tttcgtgctg ttaaacacct cgacaatttg ccgctgctct
     6781 tcgtttccaa caagcccgta ctcacgcaca aaagcggccg gctgagcgac agctgcttcc
     6841 atcatgcggg taaggtgctc cgctgtccgt tcgataaaag cggaatcaaa ggcggctccg
     6901 ttatatttaa ttttgacggt ccacgttttg cccggataca caatcaggtt gaaatcgaag
     6961 ctcgtttgtt caaatgcgct ttctacctta atcgaaaacc cgaggcggtc ttccatgctg
     7021 ccgttttcaa gctcttgatc aagcggataa ttttcaaacg cgacaagatg gtttaagaga
     7081 ttgccatcaa gagctgagcg tttttgaatc tcatagagcg gcacgtaatc gtaacgctct
     7141 gcctctactg catgctgctg aacagctgtg aaaatatcag cgaatgcagc atctcgttca
     7201 actttcaccc gcactggaat ggtgttgata aacagccccg ccatcgtttc gattccgttg
     7261 atttcagacg gtcgtcccga gacgaccgtt ccgaagatca catcatccgt aaagttgtat
     7321 ttgctgagaa caatgcccca aacggcctga aataggttag gccctgtcac ttgatggaga
     7381 tttgcggttt gctgaatacg ggcaaccagt gtttcgtccc atgtaaatga atattcttta
     7441 ttgacgtatt cgtctttttt cacaggcagg cggcccggga gcacgcttgg ctgttcaaaa
     7501 cccgccaagc gctcgctcca gtagctctcc gcttcttcat tgtcctgttt tccaagccac
     7561 ttgatgtaat cggaataagg cttgccctga ccgtttgccg gtgtcctgcc tgctctgagc
     7621 gcttcatagt tttggaacag gcttttcatg aggacgccca tgctccagcc gtccatcata
     7681 atatgatgat tgctccagac aagatacagc tgatctgcag caaggcggaa aaccgccacc
     7741 ttgaacaata tgtcttttgc caggttaaag ccttggcgct gaacgtcttg tttgtaacgc
     7801 tcaatgtgct ccttctggcg attctcgtct gcatgactga tatcctcata atgcaccttt
     7861 gttttgcgtt ccgctaacac gacctgacgc ggtttttgca gctgctgatg cacaaatacc
     7921 gtacggagaa tatcgtatga ccgaatcagt tcattgacgc ttttctcaaa gatttcaaga
     7981 tcaaaagcgc cgtgaatccc aagctctaat tgtgtgaagt acgaggaaga atgcggatca
     8041 agcatcgcat gatacagcat tccctcctgc attggtgtca gtgcgtacac cttttgaatc
     8101 gattttttgc tcatatgcca cctctattag aaaatttcca ttaatttatc cagctcatcc
     8161 agcgtcaaat cgtcgtcacc aagatcactc ggggtcagtt ccgtaccgtc ttgagatagg
     8221 caatgttcaa tgattctgag aagatgcgct ttaaagctgt cactgaatgt ttgtactgtt
     8281 ttttcatgaa attcaagaga atgataggcc agtgacatcg tcagttttcc gttttcgata
     8341 tatccgacaa catccagcgc attcggtttt tctgtttcag ggcttaatga ctgtcctgat
     8401 ggcagctctg atctcgtaaa caaacctgca tccgacattt cgtcaaactg acctaaatag
     8461 ttgaagctga catccggctg aatcgagaac gccacatctt ctttatgttc cgatgccgtc
     8521 agatagcgca gaatgccgta tccgactccc ttattcggca catgtctgat atcttctttc
     8581 atttgcttca gctggtagcc cagatcatcc gcatgcttca tatcgagcac cattgggtac
     8641 atacttgtaa accagccgac tgtgcgggaa atggtcaggt cttctatgat ttcttcacgt
     8701 ccgtgcccct ctaaatgaac atttactcga tcctgacccg tccactcctt cattgccaat
     8761 ccgaatgccg tcagcaaaat atcattcatt tctgtgtgat atgcctcatg cacctttgtg
     8821 gtgagctgtt ctgtctcttc agcagtcagc gagaattcaa ttgtctttgt atgtttcatt
     8881 cgctgatcac cagattcgcg gtctttcgga agctttgccg caacagcttg ttcttcaagc
     8941 tgtctccaat actctttttc tttcaaaaag gctttggagt tcgcgaagtc ttgcaatctc
     9001 tctgcatact ctgcaaatga atgtgttttc tgcgggagaa ccggttcatt gccttgctca
     9061 agctgtgtat atactgcggc gaaatcctcc agtaaaatcc gccacgacac gccgtcaacc
     9121 actaagtggt gaatggcaag aagcaagtgg tctccgtctt ccgcttggaa caagcctgct
     9181 tttaacaagc cgccttcctg caagtcgatg ctgctttgca aacgctccgc ttctctctct
     9241 atacggtctt cgtaatctgc agcttggccc tcaatctgga tgacctccaa ttgagcggaa
     9301 gcagcttcaa gacctctgtt gtattgaatg acgtcttcct gtccctcacg gtagaccata
     9361 cggagcgcgt catgatgctc gatcagcgcc tgcaatgttt tctccacccg ttcaggatca
     9421 aagccctttt tggcgtgaag catcacagat tggttccagt ggtgcttgtt cgtgaagttc
     9481 ttttcaaaga accagcgctg gatcggcgtc aggatgactt cgccctctac cgggccttgg
     9541 tctgcctgtt tgccttcggc acgctctaca tattgggtaa gctcttcgat cgtcggatgc
     9601 tggaagagat ctttcatttc cagcctccag ccgtgctgat tcaagcggct cgccatttga
     9661 attcctttga tggaatctcc gccaagcgag aagaaattgt cggtgacacc gacttcagac
     9721 attccaagca cgtcctgcca gatgccggca agcagctctt ccatgtcggt cgtcggcgct
     9781 ttgtattctc cgcttcccgc ttcgatgtcc ggctcaggca aggctttgcg gtccactttg
     9841 ccgtttgccg taaccggaag ctcgttcagc gtcacccaga acgccgggat catgtaatcc
     9901 ggcagggttt ccttcagtgc tgattttagt gcttctatgt cagctgtttc cggtgtgaca
     9961 tacgccgcga ttgctgtgtt gccgccttta tccttgacag ctgtcaccgc cgcatcctgc
    10021 acctcagaaa gctgggcgag ctggacttca atttctgaaa gctcgatccg gtagccgcgg
    10081 accttgacct gctggtcgat ccggccgatg tattcgatgc cgccgcccgt ccatttcacc
    10141 aaatcgccgg tgcggtacat gcgctcaccc ggcacaaacg gatcagcgac aaaccgcttc
    10201 gcggtttcgt cttctcgatt cagatagcct cgtgccagtc cgcgccccgc cacgcaaagc
    10261 tc
//
