LOCUS       NZ_CP031455            10972 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Streptomyces olivoreticuli subsp. olivoreticuli strain ATCC 31159
            chromosome, complete genome.
ACCESSION   NZ_CP031455
VERSION     NZ_CP031455.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces olivoreticuli subsp. olivoreticuli
  ORGANISM  Streptomyces olivoreticuli subsp. olivoreticuli
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8809793)
  AUTHORS   Zhang,H.
  TITLE     Complete genome sequence of Streptomyces olivoreticuli ATCC 31159,
            producer of anticancer bestatin and diverse secondary metabolites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8809793)
  AUTHORS   Zhang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2018) Tianjin Key Laboratory of Food
            Biotechnology, College of Biotechnology and Food Science, Tianjin
            University of Commerce, NO.409 Guangrong Road, Tianjin, Tianjin
            300134, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-JUN-2018
            Assembly Method                   :: SMRT Link v. 5.1.0
            Assembly Name                     :: TJCU_BT_311
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: SMRT Link v5.1.0
            Genome Coverage                   :: 145.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003391135.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 04:49:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,562
            CDSs (total)                      :: 7,459
            Genes (coding)                    :: 7,276
            CDSs (with protein)               :: 7,276
            Genes (RNA)                       :: 103
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 61 of 183
            Pseudo Genes (incomplete)         :: 150 of 183
            Pseudo Genes (internal stop)      :: 18 of 183
            Pseudo Genes (multiple problems)  :: 41 of 183
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:12:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6532471
            Orig. end                         :: 6543443
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031455.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Hongyu Zhang.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10972
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="32"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10972
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10972
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5972](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5972)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            548..1756
                     /locus_tag="DXZ73_RS29110"
     CDS             548..1756
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1184: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019525351.1"
                     /locus_tag="DXZ73_RS29110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_116213985.1"
                     /transl_table=11
                     /translation="MPLALLALAISAFGIGTTEFVTNGLLPEVADDLSVSIPTAGLLTS
                     GYAMGVVVGAPVLTAITTRMRRKHLLIGLMVLFIAGNLVAAVAPNYALLMGGRIISALC
                     HGAFFGVGSVVAANLVSPQKKAGAISLMFTGLTLANVLGMPMGTLLGQHFGWRSAFWGV
                     VALGVIGLIGIAVLVPNQPNEEGSGLRGELAAFRKPQVWLGLAVTAVGFSGLLASFTYV
                     APMMTDVAGFSDSSITWLLMLYGLGLVVGNYAGGKAADRALTATIFTLLIALAVVLTVF
                     TFTAHDKVAAAVTLFVLGGVGFGTIPPLQTRVMQLAGGPATLVSAANIAAFNLGAAVGA
                     WLGGVTIDAGLGYTSPNWVGAVITTCGIGLAVVSVGLERRRRAGALPSVTEQPVAPASA
                     PQH"
     gene            1836..2681
                     /locus_tag="DXZ73_RS29115"
     CDS             1836..2681
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (25-252). E-value:
                     4.7e-27. Score: 87.1. Matches aSDomain:
                     nrpspksdomains_DXZ73_RS29115_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018531693.1"
                     /locus_tag="DXZ73_RS29115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_116213986.1"
                     /sec_met_domain="Abhydrolase_6 (E-value: 1.1e-16, bitscore:
                     60.9, seeds: 455, tool: rule-based-clusters)"
                     /sec_met_domain="PF00561 (E-value: 5.1e-11, bitscore: 41.5,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPTVTVPVTGGVSEVEYLVTGSGPGVVLVHGTGANPGANFGPLIE
                     ALQDTFTVVAPFLSGSARTTDPGGPLTMDDLVAQVLAAADDAGLGSYHLAGHSLGAAVA
                     AAAAGARPDRVRSLLLHAGWVTTDPWLAFQFDLWQRLLRVDPDLLARVVQLTAMGPGTL
                     AALGRDEFEETAAGFTDLFEQGDMARQAALNMRIDISALLPRITAPALVIAGTHDRIVP
                     PHHQRRLAELIPRATFVELDCGHGLPFENPQLFVDTALSFLSRGQAEEVTESGSTVRSS
                     "
     aSDomain        1911..2591
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DXZ73_RS29115_Abhydrolase_1.1"
                     /evalue="4.70E-27"
                     /label="DXZ73_RS29115_Abhydrolase_1.1"
                     /locus_tag="DXZ73_RS29115"
                     /protein_end="252"
                     /protein_start="25"
                     /score="87.1"
                     /tool="antismash"
                     /translation="VVLVHGTGANPGANFGPLIEALQDTFTVVAPFLSGSARTTDPGGP
                     LTMDDLVAQVLAAADDAGLGSYHLAGHSLGAAVAAAAAGARPDRVRSLLLHAGWVTTDP
                     WLAFQFDLWQRLLRVDPDLLARVVQLTAMGPGTLAALGRDEFEETAAGFTDLFEQGDMA
                     RQAALNMRIDISALLPRITAPALVIAGTHDRIVPPHHQRRLAELIPRATFVELDCGHGL
                     PFENP"
     CDS_motif       2103..2159
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DXZ73_RS29115_0001"
                     /evalue="2.70E-05"
                     /label="NRPS-te1"
                     /locus_tag="DXZ73_RS29115"
                     /protein_end="108"
                     /protein_start="89"
                     /score="16.7"
                     /tool="antismash"
                     /translation="GSYHLAGHSLGAAVAAAAA"
     gene            complement(<2696..3565)
                     /gene="galU"
                     /locus_tag="DXZ73_RS29120"
                     /pseudo=""
     CDS             complement(<2696..3565)
                     /GO_function="GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="galU"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064: glucose-1-phosphate
                     adenylyl/thymidylyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003950226.1"
                     /locus_tag="DXZ73_RS29120"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /pseudo=""
                     /sec_met_domain="NTP_transf_3 (E-value: 4.4e-10, bitscore:
                     38.9, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRSRIRIRKAVIPAAGLGTRFLPATKATPKEMLPVVDKPAIQYV
                     VEEACAAGLGDILMVTGRNKRPLEDHFDRNFELEAALSLKRDDKRLAQVRESSGLADIH
                     YVRQGDPRGLGHAVLCAAPHVGDEPFAVLLGDDLIDPRDPLLARMTQVQERFGGSVVAL
                     MEVPAAQIGQYGCAAIRATGEGDVVRVTDLVEKPAPGTAPSNLAVIGRYVLEPAVFDVL
                     RRTEPGLGGEVQLTDALRELARGAGGPVHGVLFSGRRYDTGDRADYLRAIVRLACERED
                     LGPDFRAWL"
     misc_feature    3545..3560
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.63"
                     /tool="antismash"
     gene            complement(3685..4638)
                     /locus_tag="DXZ73_RS29125"
     CDS             complement(3685..4638)
                     /codon_start=1
                     /gene_functions="regulatory (extras) StrR_like: StrR-like
                     pathway specfic regulators"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018540612.1"
                     /locus_tag="DXZ73_RS29125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="WP_240351532.1"
                     /transl_table=11
                     /translation="MLAESDAELPPIVVHRPTMRVIDGMHRLRAAALRGDTEIEARLYD
                     GTAEDAFVFAVRLNTRHGLPLSAQERSRAAARIMTTHPQWSDRRIALVAGLSSKTVGAI
                     RRRGAGELPQPLTRVGHDGRVRPVNGAAGRRRAAEVLAEKPGASLREVAGLAGVSPGTV
                     RDVRDRLRRGDDPVPAGRRESGARRATGSPPGWGGVPRNAVRQDVPRRSLQRDYESILR
                     TLRKDPSLRFTEAGRNVLRLLDTRALFVEEQEGLISGLPGHCMPMLSDAARECANAWIE
                     FAKRLDRLERTQYPQARARAALREPGRGFEERPAAG"
     misc_feature    complement(4836..4846)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     misc_feature    4976..4990
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 18.58"
                     /tool="antismash"
     misc_feature    complement(4983..4999)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.88"
                     /tool="antismash"
     gene            complement(5001..5972)
                     /locus_tag="DXZ73_RS29130"
     CDS             complement(5001..5972)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="DXZ73_RS29130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_162890370.1"
                     /sec_met_domain="AfsA (E-value: 5.9e-17, bitscore: 60.3,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFASLQSPAAGPAAGSVPPTYVHKADPAEVRLTSWCRTGPHAFLI
                     GTRRPAGHELRHRHGESTDPLLFVETVRQTFPLLCHVAYSVPLGHQLIWQRFSYRIQND
                     AYDTQLPDGDVSLYVECFDIALRRERLTALSMSFRMMRGDVLLATAETHFAVQTPGVYQ
                     RLRGAQADATDAMLRALPAPAPLSPELVGRRDAEEVALSPAGSSPAGAHQWQLRVVTTH
                     PVFFDHPVDHAPGTLLIEAAHQAARMTDSGLPVDITALDCEFTRYVELDAPCLLSATAL
                     ETAADGSLRTRVVAEQGGEVRFAATVAHRGAPVLAATCVSAR"
     misc_feature    complement(5643..5645)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(6017..6034)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.34"
                     /tool="antismash"
     misc_feature    complement(6030..6050)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 21.27"
                     /tool="antismash"
     gene            6089..6703
                     /locus_tag="DXZ73_RS29135"
     CDS             6089..6703
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986960.1"
                     /locus_tag="DXZ73_RS29135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_240351533.1"
                     /transl_table=11
                     /translation="MQERAVQTRKLILRAAAEAFDESGYHGAQITGILGRAGLTQGAMY
                     FHFKSKEDLARTVMLEQAADLRLPERPAGLKQLVEITLALAVEMQRNVLFRAGVRLAVD
                     LEGPAVRDSSIYRWWTDRFNDELVEAHRKGELLPHVDGCELAEMLVGAYTGIQLMSQIT
                     SGRKDLPRRITSIWRYLLPGIATPEALAGITLADDGALERP"
     gene            6700..7671
                     /locus_tag="DXZ73_RS29140"
     CDS             6700..7671
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: TD (5-137). E-value: 7.8e-09. Score:
                     27.1. Matches aSDomain: nrpspksdomains_DXZ73_RS29140_TD.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008742094.1"
                     /locus_tag="DXZ73_RS29140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_116213991.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 3.8e-08, bitscore:
                     31.5, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVRRVVLTGATGFIGSKVVDALRRRTAATDTVLRVVGRNAPHRS
                     PDAGTTWVAADLTAPATLTGVCDGGDVLIHLASRVSGSPELCAAVNVDGTAALMAEAAR
                     AGVGRIVYLSTAAVYGRGPHRGVGVDEVDPSPASPASATRLLAERLVLAAGGTVLRPGL
                     VLGAGDRWVVPALGELLVRVPARWDGGAALSSVVDVHDLAGLIVAAALAPDPLPAAAFH
                     ASHPVPVRNRDLMDELAAHRILPPVPPRDWAWQQCLDRLRATDGQLGERQFTLLARDHW
                     YRSEDVWLRTGHRPGPGPLARLADSAGWYRAHLAAEPGGPPR"
     aSDomain        6715..7110
                     /aSDomain="TD"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DXZ73_RS29140_TD.1"
                     /evalue="7.80E-09"
                     /label="DXZ73_RS29140_TD.1"
                     /locus_tag="DXZ73_RS29140"
                     /protein_end="137"
                     /protein_start="5"
                     /score="27.1"
                     /tool="antismash"
                     /translation="VVLTGATGFIGSKVVDALRRRTAATDTVLRVVGRNAPHRSPDAGT
                     TWVAADLTAPATLTGVCDGGDVLIHLASRVSGSPELCAAVNVDGTAALMAEAARAGVGR
                     IVYLSTAAVYGRGPHRGVGVDEVDPSPA"
     gene            7754..8590
                     /locus_tag="DXZ73_RS29145"
     CDS             7754..8590
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1069: putative ABC
                     transporter permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982212.1"
                     /locus_tag="DXZ73_RS29145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="WP_116213992.1"
                     /transl_table=11
                     /translation="MPPLLTRRTRQRLAADAALLAVALAFAVPLVWLILASVDDGAGLR
                     VRAPEKPTLGNFSAVMNEEITFRPMLNSLLLCGAATALTMVCAALAAYPLSRFRSRFSR
                     PYLLTVLFTTCLPITAVMVPVYGLFVRIDLIDTVYGTALFMATAQLPFAIWLMKNFMDG
                     VPAVLEEAAWTDGASRLQALVRVILPLMGPGVAVVAIYSFIMLWGNFFVPFMLLLSPEQ
                     LPASVSIFTFFGNYDQVAYGQLAAFSILYSTPVILLYTLISRRLGGGFALGGAVKG"
     gene            8864..9862
                     /locus_tag="DXZ73_RS29150"
     CDS             8864..9862
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1287: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382325.1"
                     /locus_tag="DXZ73_RS29150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AraC family transcriptional regulator"
                     /protein_id="WP_240351534.1"
                     /transl_table=11
                     /translation="MTEPGVGPEAPGNMRTLVLSNDLDETREIISSAYSPYQLSVLGDP
                     DDFSAWYAEGGFPGVTVSGLQYGAETLVAPQPLDSYLLVCKLVRGRVRVISPGREEQVV
                     GAGETYVLDPYRQFQVHWAPGARMTTVRLARETVEAAAADALGVEGPLRARFGLGGPIS
                     AGAARSWAGISAAVHREVLGEGIARTSPLIAAHLTQTTAALLMETYRLITDGVDAQRPG
                     VVAHGAVRRAMALAEERPDRPLTLADLALAARVSPRALQEAFRRHLGTTPLGYLREVRL
                     GRAHADLMAGRGNPDVTVAQVACHWGFANLGRFAGLYRDRYGVPPSRTLRG"
     misc_feature    9317..9319
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 cccggtcgag cgcggcgtcg agcgtgctgt cgtgcacggg gcgcgcgatc cgcagcagtt
       61 cccggccggc cgcggtcagt tcggtgacca cgccgcgccg gtcggtcgtg cacacggacc
      121 gggcgagcag gccgcggcgc tccagccggt tgatcaggcg ggtggtggcg ctgtggctga
      181 gcgccgcggc gctgctgatc tgctgcatcc gcaggtgcca gccgccctgc cggcccaggg
      241 tctccagcac cgtgtactcc accacggaca gcccgtgttg gcgcagtgcc ttctccaagg
      301 cgatctccag cgcgcagtgg agtgccatga gggtgcgcca cccctggacc cggatttcga
      361 cggcgtcatc ggcggcgtac atggctgcct ctctgactca tcggactctc tggctcatcg
      421 gacagatcga tctcatcgga cagttcaact gcgcacgcac actaccgcct tgcgcctgta
      481 tggcgcgcgt gcaaatgttg catgcgccca ataagtgcgc acgcgcacaa ctgaggaggg
      541 tttccccatg ccactcgcgc tgctggcgct ggcgatcagc gccttcggca tcggcaccac
      601 ggagttcgtg accaacggtc tcctgcccga ggtcgccgac gacctctcgg tgtccatccc
      661 cactgccggg ctgctcacct ccggttacgc catgggcgtc gtcgtcggcg ccccggtgct
      721 caccgccatc accacccgga tgcggcgcaa gcacctgttg atcggcctga tggtgctgtt
      781 catcgccggc aacctggtcg ccgccgtcgc gccgaactac gcgctgctga tgggcggccg
      841 catcatctcc gccctgtgcc acggcgcctt cttcggcgtg ggttccgtgg tcgccgccaa
      901 cctggtcagc ccgcagaaga aggcgggcgc catctccctg atgttcaccg gcctgacgct
      961 ggcgaacgtc ctgggcatgc ccatgggcac gctgctgggg cagcacttcg gctggcgttc
     1021 ggcgttctgg ggcgtggtcg ccctcggtgt gatcggcctg atcggcatcg ccgtactcgt
     1081 gccgaaccag ccgaacgagg agggcagcgg cctgcgcggt gagctggccg cgttccgcaa
     1141 gccgcaggtg tggctggggc tcgcggtgac cgccgtcggc ttcagcggtc tgctggcgtc
     1201 gttcacctac gtcgcgccga tgatgaccga cgtcgccggg ttctccgact ccagcatcac
     1261 ctggctgctg atgctgtacg gactcggtct cgtcgtcggc aactacgcgg gcggcaaggc
     1321 cgccgaccgg gcgctgaccg ccacgatctt caccctgctc atcgcgctgg ccgtggtgct
     1381 gacggtcttc accttcaccg ctcacgacaa ggtggcggcg gcggtgacgc tcttcgtgct
     1441 cggcggtgtg ggcttcggca ccatcccgcc gttgcagacc cgtgtgatgc aactggcggg
     1501 cgggccggcc accttggtct ccgccgccaa catcgcggcg ttcaacctcg gtgcggccgt
     1561 gggcgcctgg ctcggcggtg tcaccatcga cgccggactc gggtacacct ccccgaactg
     1621 ggtcggcgcc gtcatcacga cgtgcggcat cgggctggcc gtcgtctcgg tcgggctgga
     1681 gcgccgcagg cgggcgggcg cactgccgtc cgtgaccgag cagccggtcg ctcccgcctc
     1741 cgcaccccag cactgaaccg tccgggttcc cgccgccgcg cacgcggcgg cgggaacccg
     1801 gggcggccaa cggaccgtac gcgaaaggcc ctttcatgcc cacagtcacc gttcccgtca
     1861 ccggcggcgt cagcgaggtc gagtacctcg tgacgggttc cgggcccggt gtggtgctgg
     1921 tgcacggcac cggcgcgaac cccggggcga atttcggccc gctcatcgag gcgctccagg
     1981 acaccttcac cgtcgtggca cccttcctgt ccggctcggc gcgcaccacc gacccgggcg
     2041 gccccctgac gatggacgac ctggtggccc aggtgctggc ggcggccgac gacgccgggc
     2101 tcggctccta ccacctggcc ggccactccc tgggggcggc cgtggccgcg gccgcggccg
     2161 gagcccgccc cgaccgggtc cgctcgctgc tgctccacgc gggctgggtg acaaccgatc
     2221 cgtggctcgc cttccagttc gacctctggc agcggctgct gcgcgtcgat ccggacctgc
     2281 tcgcccgcgt ggtgcagctg accgcgatgg gcccgggcac gctggcggcc ctcggcaggg
     2341 acgagttcga ggagacggcc gcgggtttca ccgacctctt cgagcaaggg gacatggccc
     2401 ggcaggccgc gctcaacatg cggatcgaca tctccgcgct cctcccgcgc atcaccgctc
     2461 ccgcgctcgt gatcgcgggc acgcacgacc ggatcgttcc cccgcaccac cagcgccggc
     2521 tcgccgagct gataccccgc gcgaccttcg tggaactcga ctgcgggcac ggcctcccct
     2581 tcgagaaccc tcagctgttc gtcgacacgg cgctgtcgtt cctcagcagg ggccaggccg
     2641 aagaggtgac ggagtccggc tccacggtcc gcagttcgtg atcggcgtag ccgagcagcc
     2701 aggcccggaa gtccgggccg agatcctcgc gctcgcaggc gagcctgacg atggcgcgca
     2761 ggtagtcggc gcggtcgccg gtgtcgtagc ggcggccgga gaacagcacg ccgtgcaccg
     2821 gccccccggc gccccgcgcc agctcgcgca gggcgtcggt gagctggacc tcgccaccga
     2881 gccccggctc cgtcctcctc agcacgtcga acaccgcggg ttcgagcaca tagcggccga
     2941 tcaccgcgag attgctcggg gcggtgccgg gcgcgggctt ctcgacgagg tcggtcaccc
     3001 gcaccacgtc gccctcgccg gtcgcgcgga tcgcggcaca gccgtactgg ccgatctgcg
     3061 cggcgggcac ctccatgagc gccaccacgc tgccgccgaa ccgctcctgc acctgcgtca
     3121 tccgggccag cagcgggtcg cgcgggtcga tgaggtcgtc gccgagcaac accgcgaagg
     3181 gctcgtcgcc gacgtgcggg gcggcgcaca gcaccgcgtg gcccagcccc cggggatcgc
     3241 cctgccgcac gtaatggata tccgcgagtc cgctggattc gcgcacttgc gcaagccttt
     3301 tgtcgtcgcg cttgagggaa agcgcggcct ccagctcgaa attgcggtcg aaatggtctt
     3361 ccagcgggcg tttgtttcgc ccggtcacca tcaggatgtc tccgagaccg gcggcgcagg
     3421 cctcttccac gacgtactgg atggcgggct tgtcgacgac ggggagcatt tccttcggcg
     3481 tcgccttggt ggcgggcaga aagcgggtgc cgagcccggc ggcgggtatg acggctttcc
     3541 tgatccgaat gcgcgatcga ctcatgccgc caccataatg agatctcgtt gcggagaaaa
     3601 cattcatcgg aaaagaaagc gcggcattcc tggtccggcc attgtgcaac cgcggaaatc
     3661 cgccggttgc acaatggccg gacctcagcc ggcggccggt ctttcctcga acccgcgccc
     3721 cggctcccgc agggccgccc gggcgcgggc ctgcgggtac tgggtgcgct ccagccggtc
     3781 cagccgcttc gcgaactcga tccaggcgtt ggcgcattca cgggcggcgt ccgagagcat
     3841 gggcatgcag tgcccgggga gaccgctgat gagcccctcc tgctcctcga cgaagagggc
     3901 gcgcgtatcc agcaggcgga gcacgttccg gcccgcttcg gtgaagcgga gggaaggatc
     3961 cttgcgcaag gtccgcagga tcgactcgta gtcccgctgc aaggatcgcc gcggcacgtc
     4021 ctgccgcacg gcattcctcg gcacgccgcc ccaaccgggc ggggagccgg tggcccgccg
     4081 ggcgccgctc tcccggcgcc ccgcgggtac ggggtcgtcg ccgcggcgca gtctgtcccg
     4141 tacgtcccgg accgttcccg gcgacacgcc cgcgagcccg gcgacttcgc gcagggacgc
     4201 acccggcttc tcggcgagca cctcggccgc ccggcggcgc cccgcggcgc cgttcaccgg
     4261 gcgtacgcgg ccgtcgtggc cgacccgggt gagcggttgg gggagctccc cggccccgcg
     4321 gcgccggatg gcgccgaccg tcttggagga gagcccggcg accagggcga tgcgccggtc
     4381 cgaccactgc ggatgggtgg tcatgatgcg ggcggcggcc cgtgagcgct cctgcgccga
     4441 gagcggcagg ccgtggcggg tgttcagccg gacggcgaag acgaaggcgt cctcggccgt
     4501 gccgtcgtag agccgcgcct cgatctcggt gtcaccgcgc agagcggcgg cacgcaggcg
     4561 gtgcatgccg tcgatcacgc gcatggtggg gcggtgcacg acgatgggcg gcagctccgc
     4621 gtcgctctcc gcgagcatcc gcacatgctc ggcgctctcc ccgccactcc ggggtgaatc
     4681 ggccgggagc agcaggccga tggtgaccgt ctccgtgacg gccgaggtta atgagtggtc
     4741 ctccagtacg tcctctaccg cccgccccgt atcttcttca gatacccgaa ggtcggcgac
     4801 cgaacgtccc acaattcccc ctagtttctc gtgccgtggc atggcatggc gtcaacatct
     4861 cccggcccgg cgccgatgcc ggggcgtgga cgtcgctgtc caggaaaggg tcgtcgctcc
     4921 gcgcggaccg ctgcgcaact ccctgctctg cgagcagcgg ccggcggagg gaaaaatccc
     4981 gactgtcgat aaattccgga ctatcgcgcc gaaacgcagg tggccgccag gaccggcgcg
     5041 ccgcgatggg cgacagtcgc cgcgaatcgc acctcgccgc cctgttccgc cacgacgcgg
     5101 gtgcgcaggg atccgtccgc ggccgtctcg agagccgtgg cactcagcag acagggggcg
     5161 tcgagctcta cgtatcgggt gaattcgcag tcgagagcgg tgatgtcgac cggcaggccg
     5221 ctgtccgtca tgcgcgcggc ctggtgggcg gcttcgatga gcagggtgcc gggcgcgtgg
     5281 tccaccgggt ggtcgaagaa gacggggtgc gtcgtgacca ctcggagctg ccactggtgc
     5341 gcaccggcgg gagaggagcc ggcgggggag agggcgactt cttcggcgtc cctccgcccg
     5401 acgagctcgg gggaaagggg ggcgggcgcg ggcagggccc gcagcatggc gtccgtggca
     5461 tcggcttgcg cgccgcgcag ccgttggtag acgcccggtg tctggaccgc gaagtgcgtc
     5521 tcggccgtgg ccagcagcac gtcgccccgc atcatgcgga acgacatcga cagggccgtg
     5581 aggcgttcgc gtcggagggc tatgtcgaag cactccacat acagggacac gtcaccgtcg
     5641 ggtaactgag tgtcatatgc atcattctgg atacggtaag agaaccgctg ccagatgagc
     5701 tgatgcccca gcggcacact gtaggcgacg tgacacagga gcgggaaggt ctgccggacg
     5761 gtctcgacga acagcagcgg atcggtggac tcgccgtgcc ggtggcggag ttcgtgcccc
     5821 gcaggccgac gggtgcctat caggaaggcg tggggacccg tgcggcacca ggacgtgagg
     5881 cggacctcgg ccggatccgc cttgtggacg taagtcggag gtacggaccc cgccgcggga
     5941 ccggcggcag gggactgcag ggacgcgaac acgactcccc ctagttctgc gctgtgccga
     6001 agtgacggaa cagcaggtcg catgtcgata acatagaggg cgttttgttt tgttgtccag
     6061 gaagttcagg ccacggatgg tgcgaccgat gcaggaaaga gctgtacaga cacgcaagtt
     6121 gatcctccgc gcggctgccg aggccttcga cgagtccggc taccacggcg ctcagatcac
     6181 cggcatcctg gggcgggccg gcctcaccca gggagcgatg tacttccact tcaagtcgaa
     6241 ggaggacctc gcccggacgg tcatgctgga gcaggccgcc gacctgcggc tgccggagcg
     6301 gccggcgggg ctgaagcagc tggtggagat caccctcgcg ctcgcggtgg agatgcagcg
     6361 caacgtgctc ttccgggccg gcgtccggct ggccgtcgac ctcgaaggac cggccgtccg
     6421 ggactccagc atctaccgct ggtggaccga ccggttcaac gacgagctcg tcgaggccca
     6481 ccgcaagggc gagttgctgc cgcacgtcga cgggtgcgag ctcgccgaga tgctggtcgg
     6541 cgcctacacg ggcatccagc tcatgtcgca gatcacctcc ggcaggaagg acctgccacg
     6601 ccggatcacc tcgatctggc ggtacctgct gcccgggatc gccacccccg aggccctggc
     6661 cgggatcacc ctcgccgacg acggcgccct ggaacggccg tgagcgtccg gcgcgtggtg
     6721 ctcaccgggg ccaccgggtt catcggctcg aaggtcgtgg acgcgctgcg acggcggacg
     6781 gccgccacgg acaccgtcct gcgcgtcgtc gggcgcaacg ccccgcaccg gtcaccggac
     6841 gcggggacca catgggtcgc cgcggacctg accgccccgg cgaccctgac cggggtctgc
     6901 gacggcgggg acgtcctcat ccacctggcc tcccgtgtca gcggatcccc cgaactgtgc
     6961 gcggcggtga acgtggacgg caccgccgcc ctcatggcgg aggccgcgcg cgcgggagtc
     7021 gggcgcatcg tctatctgtc gacggcggcg gtgtacggaa gggggccgca ccggggcgtc
     7081 ggcgtcgacg aggtcgaccc cagccccgcc tcaccggcca gcgcgacacg cctgctcgcc
     7141 gagcgtctcg tcctggcggc cggcggcacc gtcctgcggc cgggcctcgt cctgggcgcg
     7201 ggggaccgct gggtggtgcc ggccctgggc gaactgctgg tccgcgtccc cgcccgctgg
     7261 gacggcggcg ccgcactgtc gtccgtggtg gacgtccacg acctggccgg tctgatcgtg
     7321 gcggcggcgc tcgcgccgga cccgctgccc gccgcggcct tccacgcctc ccaccccgtg
     7381 cccgtgcgca accgggacct gatggacgaa ctggccgcgc accggatcct gccgcccgta
     7441 cccccgcggg actgggcctg gcagcagtgc ctggaccgcc tccgggccac ggacgggcag
     7501 ctgggcgaac ggcagttcac actcctggcg cgtgaccact ggtatcgcag cgaggacgtc
     7561 tggctccgga cgggacaccg gcccgggccg ggcccgctgg cacggctggc ggactccgcc
     7621 ggctggtacc gggcccacct ggcggccgag cccggcggac ccccgcggtg agcggcatcc
     7681 gggccgcccc gcccggtccc ggcggagccg cccccgcgct atcctccgac agccgttcgc
     7741 gtacggaggt gccatgccgc cgctgctcac ccgccgcacc cgtcaacgac tcgccgccga
     7801 cgccgcgttg ctcgccgtcg ccctcgcgtt cgccgtacca ctcgtctggc tgatcctggc
     7861 gtccgtcgac gacggggcgg ggctgcgggt gcgggcgccc gagaagccga cgctgggcaa
     7921 cttctcggcg gtgatgaacg aggagatcac cttccggccg atgctcaaca gcctgctcct
     7981 gtgcggcgcg gccaccgcgc tgaccatggt ctgcgcggcg ctcgccgcct atccgctgtc
     8041 gcgcttccgg tcgcgcttca gccggccgta cctgctgacc gtgctgttca ccacctgcct
     8101 gccgatcacc gcggtcatgg tgccggtcta cgggctgttc gtacggatcg acctcatcga
     8161 caccgtctac ggcaccgccc tgttcatggc aaccgcccaa ctgcccttcg ccatctggct
     8221 gatgaagaac ttcatggacg gtgtgccggc cgtcctggag gaggccgcct ggacggacgg
     8281 cgcgagcagg ctccaggcgc tggtcagggt catcctgccg ctgatgggac ccggggtggc
     8341 cgtcgtggcg atctacagct tcatcatgct ctggggcaac ttcttcgtgc ccttcatgct
     8401 gctgctctcg cccgaacaac tcccggcgtc cgtcagcatc ttcacgttct tcgggaacta
     8461 cgaccaggtg gcgtacgggc agctcgccgc gttctcgatc ctctactcga cgccggtgat
     8521 cctgctctac acgctgatct cccggcgcct cggcggcggc ttcgcgctgg gcggcgccgt
     8581 caagggctga tccctcaagg gccggggtga caggacgcag aagaaccccg ccgtctgtcc
     8641 gctgacggcg gggcaccccc tccggtgggg atccggccct ggatgtcccc cgtggatcca
     8701 gggccgcgcc cgcgcacggc gccgggccct gtcccgaaag agctgtgggg acgacccccg
     8761 ccttgactgc ggacaagcag aacggtacta acggggccac ctgtcggcta ctcgtcaaac
     8821 gcggctcctg gccggagaac gcgggactcc cgaggaacgc ctgatgaccg aacccggagt
     8881 ggggccagag gcccccggca acatgcgcac gctcgtactc agcaacgatc tcgacgaaac
     8941 ccgcgagatc atcagctccg cctacagccc ctaccagctc agtgtgctcg gcgaccccga
     9001 cgacttctcc gcgtggtacg ccgagggcgg cttccccggt gtcaccgtct ccggcctcca
     9061 gtacggggcc gagaccctgg tcgcgcccca gccgctcgac tcctacctcc tcgtctgcaa
     9121 gctcgtccgg ggccgggtgc gggtgatctc ccccgggcgg gaggagcagg tcgtgggggc
     9181 cggcgagacc tatgtgctcg acccctaccg ccagttccag gtgcactggg cgcccggcgc
     9241 gcggatgacc accgtccggc tcgcgcggga gaccgtggaa gcggccgcgg ccgacgcgct
     9301 cggcgtggag ggcccgttac gcgcccgctt cgggctcggc ggcccgatct ccgccggggc
     9361 cgcccggagc tgggccggga tctccgccgc cgtgcaccgc gaagtgctcg gcgagggcat
     9421 cgcgcgcacc agcccgctga tcgccgccca tctgacccag accaccgccg ccctgctgat
     9481 ggagacctac cggctgatca ccgacggagt ggacgcccag cggccgggcg tcgtcgcgca
     9541 cggcgcggta cgccgggcca tggccctcgc ggaggagcgc cccgaccggc cgctcaccct
     9601 cgccgacctc gcactggcgg cccgcgtcag cccacgtgcc ctgcaggagg cgttccgccg
     9661 gcacctgggg acgacaccgc tcggctacct ccgcgaggtg cggctggggc gggcacacgc
     9721 ggatctcatg gcggggcgtg gcaacccgga cgtcaccgtg gcccaggtcg cctgccactg
     9781 gggcttcgcc aacctgggcc gcttcgcggg cctgtaccga gatcggtacg gggtgccgcc
     9841 gtcgcgtacg ttgcgtgggt gagcgcgcgc tgtcgcctgc ggcgggcttg ttccccaccc
     9901 cgccccttcc cgaaaccggg gagaccccgg acccccagcg tcctgcgggc gcgtcctcaa
     9961 tcgccggacg ggctgaattc agcccgtccg gcgattgagg acagcgcgcg aagcgtgctc
    10021 cggggtctgg ggcgaagccc cagttacggg aaggggcggg gtaggggaac aaagcccgcc
    10081 gggccgcacc cgagcacgaa tccagggcgc agccccggcc gcggcggagc cgagtatgag
    10141 acccgccccg taggggcccc catgcgccct gaggatgacg ccgtacatct ccggtactac
    10201 ggccgacagg ccgcctcgcc cttttgaccg gtacccgatt ccggccgtac ggctgaggcc
    10261 cggcaacccc ccgccaccca gacttggagg agttgattcc ggccattgcg gccaccccga
    10321 gcacccatga gaacgccccc tccggaggag cccccgtgtc gtcgcaccga accgccaccc
    10381 ccacccgcgt ggcagcccgc gccaccggcc tgagcaaggt ctacggccac ggggagacgc
    10441 aggtgcgcgc cctggacgcg gtctccgtgg agttccgccg cggcgagttc accgcgatca
    10501 tgggcccctc cggctccggc aagtcgacgc tgatgcactg catggcgggg ctcgactcgg
    10561 tctcctccgg ctccgcgcag atcggcgagg ccgagctcgc gtcgctcgac gaccgccggc
    10621 tgacgaggct ccgccgggac aagatcggct tcgtcttcca ggcgttcaac ctcctcccga
    10681 cgctgaccgc gctggagaac atcaccctgc ccatggacat cgcgggccgt aagcccgacg
    10741 cccagtggct gaacatggtc gtcgacaccg tcggcctctc cgggcggctc gcacaccgcc
    10801 cgagccagct gtccggcggc cagcagcagc gcgtcgccgt ggcccgcgcg ctcgccgccc
    10861 agcccgagat catcttcgcg gacgagccga ccggcaacct cgactcccgc tccggtgccg
    10921 aactcctggg cttcctgcac gagtccgtac gcgcgctcca gcagacgatc gt
//
