LOCUS       NZ_CP031455            10438 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Streptomyces olivoreticuli subsp. olivoreticuli strain ATCC 31159
            chromosome, complete genome.
ACCESSION   NZ_CP031455
VERSION     NZ_CP031455.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces olivoreticuli subsp. olivoreticuli
  ORGANISM  Streptomyces olivoreticuli subsp. olivoreticuli
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8809793)
  AUTHORS   Zhang,H.
  TITLE     Complete genome sequence of Streptomyces olivoreticuli ATCC 31159,
            producer of anticancer bestatin and diverse secondary metabolites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8809793)
  AUTHORS   Zhang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2018) Tianjin Key Laboratory of Food
            Biotechnology, College of Biotechnology and Food Science, Tianjin
            University of Commerce, NO.409 Guangrong Road, Tianjin, Tianjin
            300134, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-JUN-2018
            Assembly Method                   :: SMRT Link v. 5.1.0
            Assembly Name                     :: TJCU_BT_311
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: SMRT Link v5.1.0
            Genome Coverage                   :: 145.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003391135.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 04:49:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,562
            CDSs (total)                      :: 7,459
            Genes (coding)                    :: 7,276
            CDSs (with protein)               :: 7,276
            Genes (RNA)                       :: 103
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 61 of 183
            Pseudo Genes (incomplete)         :: 150 of 183
            Pseudo Genes (internal stop)      :: 18 of 183
            Pseudo Genes (multiple problems)  :: 41 of 183
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:12:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5655421
            Orig. end                         :: 5665859
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031455.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Hongyu Zhang.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10438
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="30"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10438
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10438
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5438](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5438)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    21..35
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 24.85"
                     /tool="antismash"
     misc_feature    complement(366..381)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 20.89"
                     /tool="antismash"
     misc_feature    367..380
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.59"
                     /tool="antismash"
     misc_feature    complement(404..418)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.25"
                     /tool="antismash"
     misc_feature    complement(411..425)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.91"
                     /tool="antismash"
     gene            complement(481..843)
                     /locus_tag="DXZ73_RS25065"
     CDS             complement(481..843)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /locus_tag="DXZ73_RS25065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="WP_116213308.1"
                     /transl_table=11
                     /translation="MIGSGVLAGLLENWRVSFDEHRVKDIVGLFAEDALFQGLSPRLLV
                     GRAEITTYYENVPAGMTAAVELLHSRTVGAEAVSGFATVTFTDASGVKLPVRLSVLAQH
                     LDDAWLIRQYHVSKLG"
     misc_feature    869..886
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.59"
                     /tool="antismash"
     misc_feature    918..931
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.07"
                     /tool="antismash"
     misc_feature    complement(921..934)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.08"
                     /tool="antismash"
     gene            1116..1973
                     /locus_tag="DXZ73_RS25070"
     CDS             1116..1973
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /locus_tag="DXZ73_RS25070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_162890322.1"
                     /transl_table=11
                     /translation="MTPPDDPDILVPVAPEVALAELVRPIEQSLERQRMDAQSLAGAFV
                     PVHAIYTAAKREHQGWAMPIHGERVINSTLVHAVRSCQDELMTVQPGGERLVYDLEELD
                     HVRRRTRHRSLYQHAVRTQSATMRHIEDIVGAGGEVRTLDHVFDRLVVIDRAVAYVPGD
                     DARRTTALEIRHPDIVSFLVQVFEEAWERATPVVAAAPRSLEPVVTDETQRAIMRMLVA
                     GYTDDAIARRLGISRRTVAGRVSRISASLRSRSRAQLGYLIATTGMLPMDDGLPEEPEE
                     ELSA"
     gene            complement(2066..3562)
                     /locus_tag="DXZ73_RS25075"
     CDS             complement(2066..3562)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1038: phenylalanine-specific permease"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004956163.1"
                     /locus_tag="DXZ73_RS25075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="amino acid permease"
                     /protein_id="WP_116213310.1"
                     /transl_table=11
                     /translation="MAAQATKAPAEAGHPSAGSPAVDPAPRAADQKEGGELGNGLRPRH
                     LTMLALGGVIGAGLFVGSGTGLRVAGPAIIVSYALAAGLALLVMRMLGELAANMPASGS
                     FSVYAERALGKWAGFTAGWLYWWVTVIAIAIELIAASSILHQWLPAVPQWAFALAALAV
                     FTIANMLSVASFGEAEFWFAGIKVAAIIAFLALGVTAMCGALPGVDSPGLDNITGHGGF
                     VPQGWNSIVAGLLAVVFAFGGMEVITMAAAESGDPARSVTKAVNSAIWRISLFYLGSIA
                     VIVALLPWDSAVPGRSPYVAVLDRLGVPGAGRLIEVVVLIALLSAMNANLYGASRMAHS
                     LAARGDAPRALLRTSRRGAPRLAVAASAAFGFCSIALDYGWPERILPFLAKAMGGAMLF
                     MWLTVAVAHLVLRRRLERDSPEALVVRMRGYPYLSWTAVVGIVTVLVLMALDVKARGQL
                     GWSALLVLVLLGISAVRSMPFGSAPKGRGEPRDQPRPTRG"
     gene            complement(3627..4082)
                     /locus_tag="DXZ73_RS25080"
     CDS             complement(3627..4082)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF022164.4"
                     /locus_tag="DXZ73_RS25080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MoaF N-terminal domain-containing protein"
                     /protein_id="WP_116213311.1"
                     /transl_table=11
                     /translation="MRIRHTVPAVAAAALIALGAAGTAQAAPADPAAVPAASVRSTTPA
                     FAGHTYVLTLDNGAVLRNSYSADGKTLDWTALAGPMEGRSGQEKLTVREVGEGLYFVNW
                     VEASGMTVSHVMDLRTKTVSIYWTYGTPDGGRAGELHTAKLERVSAQ"
     gene            complement(4122..4988)
                     /locus_tag="DXZ73_RS25085"
     CDS             complement(4122..4988)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007262496.1"
                     /locus_tag="DXZ73_RS25085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase family protein"
                     /protein_id="WP_116213312.1"
                     /transl_table=11
                     /translation="MYTRKNQKNLTSAEKKKFVAALLELKRRGRYDEYVKLHGKYFVSD
                     GEAGVRVGHMSPSFFPWHRRFLLEFERDLQSVDPDVTLPYWDWTVDQSPTSSLWADDFL
                     GGTGRASDGQVMTGPFAHAAGHWSIDVGVTEEKFLMRDLGRPAAPVALPTRQELAHAVD
                     DPVYDSAPWDSTVTTAGFRNKMEGWTVGADRGWTNHNRVHLWVGGQMTGGTSPNDPVFW
                     LHHTFIDLIWARWQRKHPGASYEPRKKVPAGDPQFGRVISLDEPMDPWGARPSDLLDHE
                     RYYRYAE"
     gene            complement(5001..5438)
                     /locus_tag="DXZ73_RS25090"
     CDS             complement(5001..5438)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613014.1"
                     /locus_tag="DXZ73_RS25090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_116213313.1"
                     /sec_met_domain="melC (E-value: 2.6e-46, bitscore: 156.1,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVTRRNVLMTAFTAAVVTGTGAALAPVVLADQDDGAAAPNGGP
                     DLATDSFEETYRGRRIQGRTLSTCGARPAAYRKGAAAAPDVPGIEISIDGRPLHVMRRA
                     DGTYLSLVNHYESFPTPLAVARAAVDRIGTAQLSLTTTHHH"
     gene            complement(5479..7011)
                     /locus_tag="DXZ73_RS25095"
     CDS             complement(5479..7011)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024846.4"
                     /locus_tag="DXZ73_RS25095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="WP_162890323.1"
                     /transl_table=11
                     /translation="MTMNASDHPRMRIGIVGGGAAAVCLLDRIARTPGRGPGSITVFEP
                     SDNLWRGRAYQRDVDSVLANIIPSEMSATTDDPESFLRWLGEAGFPHGVGDYAADGHFP
                     PRHVFGSYLERTAEQARAELALGDWPVTVVRDAVVGARQRADDVVLTTAGGRAESFDYV
                     ICSVGAGRPADHYDLAGTPGFHGDPYPLTRTLPAIPETADVTVLGTGLAAVDVVVSLAA
                     RGHRGRITMASRNGALPAVRQRPIHPELRHFTPASLRALAAGGPDGRIGLDELLGLLER
                     ELAEHGAGPSGVLAELFSIDREDPVARLIRQYENIDSTDLGMRIMQHAIPESGPDAWTL
                     LRAEDQEFIRENHYRAIMSLCCPMPPANARTLLRLREEGLLRVTGGLWSVKPLATEGFH
                     IAVGDDSYETDVVVNAVSAPSHRVPVEAHPFVESLVEEEAAEYHPSGGLHIEPSTSRIA
                     GHGRVHRRMHAIGDLGAGTLFFTSGMPSVAERADDIVRAIAEDIEAARIGTA"
     gene            complement(7050..8042)
                     /locus_tag="DXZ73_RS25100"
     CDS             complement(7050..8042)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="DXZ73_RS25100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_162890324.1"
                     /transl_table=11
                     /translation="MSSSAAPRVEARKILIIVAVVQFVIGGWFSWTASHAKPRDLPVLV
                     VSASEDLADSLRKAAPGAYEITTAADTAAADRKLEDRAAYGAFIATPKGIELHVASAAS
                     PTVAQTLTQQAQQLQDKPVAVTDVVPADADDKHGSGMAMGFLPLLITAVIAGLLFVLKV
                     RDARAKLLGLVGFAVLSGLLSSLMLTHLSGVLPGNYFVNAGVIALLELAMVGVITGFGS
                     LVGPPGIAVGAVLMFVVGNPLSGMASAPEMLPQPLGAIGQFLPAGAAATLFRSVGSFHG
                     AGGGTPLLVLVVWSLIGLALLFLSARRAAGRTAPEAAPAPDAPVTASVG"
     gene            complement(8073..8873)
                     /locus_tag="DXZ73_RS25105"
     CDS             complement(8073..8873)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016473237.1"
                     /locus_tag="DXZ73_RS25105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class II aldolase/adducin family protein"
                     /protein_id="WP_205519221.1"
                     /transl_table=11
                     /translation="MSTDTLANHLADTHTGLPIPADREFTSPDEERRHRKQRLAAAMRL
                     FGKAGFAEGISGHISVRDPEDLDTFWVNPFGLSFNQVRVRDLIRVDAEGRVVDGTGKVN
                     PSAFTIHSKIHERPEANAIAHGHTVHARALGALERLLEPYDQESAAFYQDQVLYGDYDG
                     PAISVEHGQDIADRLGDRRAMLLRHHGLITVGGSLEEAVHWFFTYESCAQVQLLALAAG
                     KPVPMSHEQALLARDGFGDEQLGRFSFQIMWDEITADQPDLLDE"
     misc_feature    complement(8867..8880)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
     gene            complement(9219..10043)
                     /locus_tag="DXZ73_RS25110"
     CDS             complement(9219..10043)
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: oMT (61-213). E-value: 2.1e-30. Score:
                     97.1. Matches aSDomain: nrpspksdomains_DXZ73_RS25110_oMT.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016644836.1"
                     /locus_tag="DXZ73_RS25110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="methyltransferase domain-containing protein"
                     /protein_id="WP_116213317.1"
                     /transl_table=11
                     /translation="MTQDNVARTYYDTGDVDGFYAQAWGGENIHVGIYTDEREAIGVAA
                     RRTVERMAAKAADALGPGRAILDLGSGYGGAARQLAERFGCRVTGLNISEVQNRRHREI
                     NAERGLDGLIDVVTGSFQDVPAPDGAYDVVWSQDALCHSPDRATVLREAVRVLKPGGHL
                     VLTDFMAVEDAPADELRPLTDRLTVTGFATPAFYDEQLRSLPLSDVSFEDLGEHLQTHY
                     ERLTDEVRDPSPTLVASVSAAYLEGLRESLPQVAAACAKGLLKWGIFHARRV"
     aSDomain        complement(9405..9860)
                     /aSDomain="MT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DXZ73_RS25110_oMT.1"
                     /domain_subtypes="oMT"
                     /evalue="2.10E-30"
                     /label="DXZ73_RS25110_oMT.1"
                     /locus_tag="DXZ73_RS25110"
                     /protein_end="213"
                     /protein_start="61"
                     /score="97.1"
                     /tool="antismash"
                     /translation="PGRAILDLGSGYGGAARQLAERFGCRVTGLNISEVQNRRHREINA
                     ERGLDGLIDVVTGSFQDVPAPDGAYDVVWSQDALCHSPDRATVLREAVRVLKPGGHLVL
                     TDFMAVEDAPADELRPLTDRLTVTGFATPAFYDEQLRSLPLSDVSFED"
ORIGIN
        1 gggcgagaag ccacaggacc aggccgaccg gcgcttcacc ggcggcagca acgcggacct
       61 gcactaccgc gtggccgcct acgcgccggg cgacgcggag aagggcatcg cgcggctcaa
      121 gacgctcccg caggcctgcg atcagttcga tgtcgcctac tcgggcggcg tcaagggctc
      181 cggccaggtt gtcacgtcca agctgcccgg cgtgggcgag ggcgacgtgg gtctgcagct
      241 gacgctcaag accacgctca agggcctccc ggcgaccctc accctggaca cagccgccgt
      301 ccgcgtcggc ccgaacgccc tcaccctcac caacggcggc ctcggcggag cggtccaccc
      361 tgtcaccgaa caagcggtca agcagggcac tcagcagctc aaggatgttc tgaagaagca
      421 ttccacttga gcgttcgagg cgcggggctg gcggtgtcgg gcaggccgcg tgacagatgg
      481 tcagccgagc ttcgacacgt ggtactgccg gatgagccag gcgtcgtcca agtgctgggc
      541 cagcaccgag aggcgtacgg gcagcttgac ccccgacgcg tccgtgaagg tgaccgtcgc
      601 gaagccgctc acggcctcgg cgccgaccgt gcgggagtgg agcagctcca cggcggccgt
      661 catgccggcc gggacgttct cgtagtacgt ggtgatctcg gcgcggccca ccagcagccg
      721 gggggagaga ccctggaata gggcgtcctc ggcgaagagg ccgacgatgt ccttcacccg
      781 gtgttcatcg aaggagaccc gccagttctc cagcaggccc gccaatacac cagagccgat
      841 cattcgctcc tcattccctg cgtatcgaag gttacgtgtt tgtgcaacca gcagtatgtg
      901 tcgcactaag cccctacatc tttgattgaa aggtgcataa ttgcgccatg caggatcacg
      961 caaagcagtt gggggattgc tgcagcgggt cgcaccctgc cgacggggcc gatgccctgt
     1021 gcggcgaggg tctgacgcgc tatcggcagg ccgtggtcaa gggatgtaca ccgaaggagg
     1081 gggctccgca gtgtctgttg cggaaggggt tgctggtgac gccaccagac gacccggaca
     1141 ttttggtacc tgttgcaccc gaggtggcgc tcgctgagct cgtgcgcccc atcgagcagt
     1201 cgctggagcg gcagcggatg gacgcgcaga gcctcgccgg tgccttcgtc ccggtgcacg
     1261 cgatctacac cgcggccaag cgggaacacc agggctgggc gatgccgatc cacggggaac
     1321 gggtcatcaa ctccacgctg gtgcacgccg tccgctcgtg ccaggacgag ctgatgaccg
     1381 tacagccggg gggcgaacga ctggtctacg acctggagga gctggatcac gtacggcgcc
     1441 ggacccggca ccggagcctg taccagcacg cggtgcgcac ccagtccgcg acgatgcggc
     1501 acatcgagga catcgtgggg gcgggcggcg aggtccgcac gctggaccac gtcttcgacc
     1561 ggctcgtcgt catcgaccgg gcggtggcgt acgtgccggg cgacgacgcc cggcgcacca
     1621 cggcgctgga gatccggcac cccgacatcg tctcgttcct cgtccaggtc ttcgaggagg
     1681 cgtgggagcg ggccaccccc gtggtcgccg cggccccgcg ctccctggag ccggtcgtca
     1741 ccgatgagac gcagcgggcc atcatgcgga tgctggtggc cggttacacc gacgacgcga
     1801 tcgcccgccg gctgggcatc agccggcgga ccgtggccgg gcgggtgagc cggatctccg
     1861 cgtcgctgcg cagccgcagc cgggcgcagc tggggtatct catcgcgacg acggggatgc
     1921 tgccgatgga cgacggactg ccggaggagc cggaggagga gctctcggcc tgaccggacg
     1981 agccgtggcc gggcgggtcg ccacccgccc ggccacggct cgtccccggg cccggccctg
     2041 agcgttccgc cggaagtccg gtacgtcagc cgcgggtggg ccgtggctgg tcgcgcggtt
     2101 ccccgcgccc cttcggggca ctgccgaacg gcatgctgcg taccgccgag atgcccagca
     2161 gcaccagcac cagcagggcc gaccacccga gctggccacg ggccttgacg tcgagggcca
     2221 tcagcaccag caccgtcacg atgcccacca cggcggtcca ggacagatac ggatagccgc
     2281 gcatccgcac caccagcgcc tcgggcgagt cgcgttcgag gcggcggcgc agcaccaggt
     2341 gggccaccgc cacggtcagc cacatgaaga gcatcgcccc gcccatggcc ttggccagga
     2401 acggcaggat ccgctcgggc catccgtagt cgagcgcgat ggagcagaac ccgaaggccg
     2461 cggacgccgc caccgcgagc cggggcgcgc cgcgccgcga ggtgcgcagc agcgcccggg
     2521 gggcgtcgcc gcgggcggcg agcgagtggg ccatgcgcga ggcgccgtag aggttggcgt
     2581 tcatcgccga cagcagggcg atgaggacga cgacctcgat gagccggccc gcgccgggga
     2641 cgccgagccg gtcgagcacg gcgacgtacg ggctgcggcc gggcacggcg gagtcccagg
     2701 gcagcagggc gacgatcacc gcgatcgagc cgaggtagaa gaggctgatg cgccagatgg
     2761 cgctgttgac ggccttggtg accgagcggg cggggtcgcc ggactcggcc gccgccatgg
     2821 tgatgacctc catgccgccg aaggcgaaga cgacggcgag cagcccggcg acgatgctgt
     2881 tccagccctg cgggacgaag cccccgtggc cggtgatgtt gtccaggccg ggggagtcca
     2941 ccccgggcag ggcgccgcac atcgcggtga cgcccagtgc gaggaacgcg atgatcgcgg
     3001 cgaccttgat gccggcgaac cagaactcgg cctcgccgaa ggacgcgacg gagagcatgt
     3061 tcgcgatggt gaagacggcc agggcggcca gggcgaaggc ccactgcggg acggcgggca
     3121 gccactggtg gaggatgctc gacgcggcga tcagctcgat cgcgatggcg atgacggtca
     3181 cccaccagta cagccagccg gcggtgaaac cggcccactt gcccagggcg cgctcggcgt
     3241 agacggagaa cgagccgctg gcgggcatgt tcgccgccag ctcgccgagc atccgcatca
     3301 ccagcagggc gaggcccgcg gcgagcgcgt acgagacgat gatggcgggt ccggcgacgc
     3361 gcagcccggt gccggagccc acgaacagcc cggcgccgat gacgccgccg agggcgagca
     3421 tggtcagatg ccgggggcgc aggccgttgc cgagctcccc gccttccttc tggtcggccg
     3481 cccgcggtgc cgggtcgact gccggggatc cggccgacgg gtgtccggcc tcggccggtg
     3541 ccttggtcgc ttgagcggcc atgggtgata tcgctcctgg ggagagaaag gcgtgggcgg
     3601 ggcccgccgg gccccgccca cggacatcac tgtgcggaga cgcgctccag cttcgcggtg
     3661 tgcagctcgc cggcgcggcc gccgtcgggg gtgccgtacg tccagtagat gctcacggtc
     3721 ttggtgcgca ggtccatgac gtggctgacg gtcatgccgg acgcctcgac ccagttgacg
     3781 aagtacaggc cctcgccgac ctcgcggacg gtgagcttct cctggccgct cctgccctcc
     3841 atggggccgg cgagggcggt ccagtcgagg gtcttgccgt cggcggagta ggagttgcgc
     3901 aggacggcgc cgttgtcgag ggtcagcacg taggtgtgcc cggcgaaggc gggcgtggtg
     3961 ctgcggacgg acgcggcggg cacggcggcc gggtcggcgg gggcggcctg ggcggtgccc
     4021 gccgcgccga gggcgatcag ggcggctgcc gcgacagccg ggaccgtgtg acgaatgcgc
     4081 atggtgattc ctcttctcaa ctcagtggga aatgaagggt gttattccgc gtagcggtag
     4141 tagcgctcgt ggtccaggag gtcggacggc cgcgcgcccc acgggtccat gggttcgtcg
     4201 aggctgatga cccgcccgaa ctgggggtcc ccggcgggca ccttcttgcg cggctcgtag
     4261 ctcgcgccgg ggtgcttgcg ctgccagcgg gcccagatca ggtcgatgaa ggtgtggtgg
     4321 agccagaaga ccgggtcgtt gggcgaggtg ccgccggtca tctggccgcc gacccacagg
     4381 tggacgcggt tgtggttggt ccagccccgg tcggcgccga cggtccagcc ctccatcttg
     4441 ttgcggaagc ccgccgtggt gacggtggag tcccaggggg cgctgtcgta gaccgggtcg
     4501 tcgacggcgt gggccagctc ctgcctggtg ggcagggcga cgggggcggc gggacggccg
     4561 aggtcccgca tcaggaactt ctcctccgtc acacccacgt cgatcgacca gtggccggcc
     4621 gcgtgggcga acgggcccgt catgacctgc ccgtcggagg cccggccggt gccgcccagg
     4681 aagtcgtcgg cccacagcga ggaggtgggc gactggtcga cggtccagtc ccagtacggc
     4741 agggtcacgt ccgggtcgac ggactgcagg tcgcgctcga actccagcag gaaccggcgg
     4801 tgccagggga agaacgaggg ggacatgtgg ccgacccgca cgcccgcttc gccgtcggag
     4861 acgaagtact tgccgtgcag cttgacgtat tcgtcgtagc ggccccggcg cttgagctcc
     4921 agcagggcgg cgacgaactt cttcttctcg gcgctggtga gattcttctg gttcttgcgt
     4981 gtgtacaccg gtgctccgga tcagtggtgg tgcgtcgtgg tgagcgacag ctgtgcggtg
     5041 ccgatccggt cgacggcggc gcgggcgacg gcgagcggcg tggggaacga ctcgtagtgg
     5101 ttcaccaggc tcagataggt gccgtcggca cggcgcatca cgtgcagcgg gcgcccgtcg
     5161 atgctgatct cgatgccggg cacgtcgggc gcggcggcgg cgcccttgcg gtaggcggcg
     5221 ggccgcgcgc cgcaggtgga cagcgtcctg ccctggatgc ggcggccccg gtaggtctcc
     5281 tcgaaggagt ccgtcgccag gtccgggccc ccgttcgggg cggccgcgcc gtcgtcctgg
     5341 tcggccagga cgacgggcgc gagcgccgcg ccggtaccgg tgacgacggc ggcggtgaac
     5401 gccgtcatga gcacatttct gcgggtgacc gtggtcatcc tggtgtttcc cccgtgattt
     5461 tgatggtgtt cgcgtggttc aggcggtgcc gatgcgcgcg gcctcgatgt cctcggcgat
     5521 ggcgcggacg atgtcgtcgg cgcgctcggc caccgagggc atgccggagg tgaagaacag
     5581 cgtcccggcg cccaggtcgc cgatcgcgtg catgcgccgg tgcacccggc cgtggccagc
     5641 gatccggctg gtggacggct cgatgtgcag accgccggag gggtggtact cggccgcctc
     5701 ctcctcgacg agcgactcga cgaacgggtg ggcctccacc gggacccggt gggagggcgc
     5761 gctgaccgcg ttgaccacca cgtccgtctc gtagctgtcg tcaccgacgg cgatgtggaa
     5821 gccttccgtg gcgagcggct tgacgctcca cagcccgcct gtcacccgca gcaggccctc
     5881 ttcgcgcagc ctgagcagcg tgcgggcgtt ggccgggggc atcgggcagc acaggctcat
     5941 gatcgcccgg tagtggttct cgcggatgaa ctcctggtcc tccgcgcgca gcagcgtcca
     6001 ggcgtcgggg ccgctctccg ggatcgcgtg ctgcatgatc cgcatgccca ggtcggtgga
     6061 gtcgatgttc tcgtactggc ggatcagccg ggcgacggga tcctcccggt cgatggagaa
     6121 cagctcggcc agcactccgg acgggcccgc gccgtgctcc gccaactccc gctccagcag
     6181 cccgagcagc tcgtccagcc cgatccggcc gtcggggccg ccggccgcca gcgcccgcag
     6241 ggaggcgggg gtgaagtgcc gcagctccgg gtggatgggg cgctgccgga ccgccgggag
     6301 ggcgccgttg cgggacgcca tggtgatccg gccccggtgg ccgcgcgcgg ccagggagac
     6361 gacgacgtcg acggcggcga ggccggtgcc gagcacggtg acgtcggcgg tctccgggat
     6421 cgcgggcagc gtccgcgtca gcgggtacgg gtcgccgtgg aacccgggcg tgcccgccag
     6481 gtcgtagtgg tcggccgggc ggcccgcgcc gaccgagcag atcacgtagt cgaagctctc
     6541 ggcgcggccc ccggcggtgg tcaggacgac gtcgtccgcc cgctgccggg ccccgacgac
     6601 ggcgtcccgg acgacggtca ccggccagtc gccgagggcg agttcggcgc gggcctgctc
     6661 ggccgtccgc tccagatagc tgccgaagac atggcggggc gggaagtggc cgtcggccgc
     6721 gtagtccccc acgccgtggg ggaaccccgc ctcgccgagc cagcgcagga agctctccgg
     6781 gtcgtccgtg gtggccgaca tctccgaggg gatgatgttg gccagcacgg agtcgacgtc
     6841 acgctggtag gcgcggcccc gccacaggtt gtccgagggc tcgaagacgg tgatcgaccc
     6901 ggggccgcgc cccggagtgc gggctatccg gtccagcagg cagacggctg ccgctccgcc
     6961 gccgacgatg ccgatgcgca tccgcgggtg gtcacttgcg ttcatggtca tggtcatctt
     7021 tcgggaacgg aggagcgttg aggagggcgt cagcccacgg aggccgtgac cggggcatcg
     7081 ggcgccgggg ccgcctcggg ggccgtccgg ccggccgcgc ggcgggcgct caggaacagc
     7141 agggccaggc cgatcagcga ccagaccacc agcaccagca ggggtgtgcc gccgcccgcg
     7201 ccgtggaagg agccgaccga gcggaagagc gtggcggccg cgcccgcggg caggaactgg
     7261 ccgatggcgc ccagcggttg gggcagcatc tcgggggccg acgccatgcc ggacagcggg
     7321 ttgccgacga cgaacatcag caccgcgccc accgcgatcc ccggcgggcc caccagcgag
     7381 ccgaagccgg tgatgacacc gaccatggcc agctccagca gcgcgatgac gcccgcgttg
     7441 acgaagtagt tgccgggcag cacgccgctg aggtgggtca gcatcagcga gctgagcagg
     7501 ccggacagca cggcgaagcc gacaagcccg aggagcttgg cgcgggcgtc gcgcaccttc
     7561 agcacgaaca gcaggcccgc gatgacagcg gtgatcagca gcggcaggaa gcccatggcc
     7621 atgccggagc cgtgcttgtc gtcggcgtcg gccgggacga cgtcggtgac ggcgaccggc
     7681 ttgtcctgga gctgctgcgc ctgctgggtc agggtctgcg ccaccgtggg cgaggcggcc
     7741 gaggccacgt ggagctcgat gcccttgggc gtggcgatga aggcgccgta cgccgcgcgg
     7801 tcctcgagct tgcggtcggc cgcggcggtg tcggcggccg tggtgatctc gtacgcgccg
     7861 ggcgcggcct tgcgcagcga gtccgcgagg tcctcgctcg cggacaccac gagcaccggc
     7921 aggtcacgcg gtttggcgtg cgacgccgtc cacgagaacc agccgccgat cacgaactgc
     7981 acgaccgcga cgatgatcag aatcttgcgc gcttccacgc gcggagcggc tgatgaactc
     8041 atcggttcct ccaacttcct ggcgtgggtg ggttattcgt cgagcaggtc gggctggtcg
     8101 gcggtgatct cgtcccacat gatctggaag ctgaaccggc cgagctgctc gtcgccgaag
     8161 ccgtcgcgcg ccagcagggc ctgctcgtgg ctcatcggca cgggcttgcc cgcggcgagg
     8221 gcgaggagct ggacctgggc gcagctctcg taggtgaaga accagtgcac cgcctcctcc
     8281 agcgagccgc cgaccgtgat caggccgtgg tggcggagca gcatcgcgcg ccggtcgccg
     8341 agccggtcgg cgatgtcctg cccgtgctcg accgagatcg ccgggccgtc gtagtcgccg
     8401 tagaggacct ggtcctggta gaacgccgcc gactcctggt cgtagggctc cagcaggcgc
     8461 tccagggcgc cgagtgcgcg ggcgtgcacg gtgtggccgt gcgcgatggc gttggcctcg
     8521 gggcgctcgt ggatcttgga gtggatggtg aaggcgctgg ggttgacctt gcccgtgccg
     8581 tcgacgaccc ggccctcggc gtcgacgcgg atcaggtcgc gcacccgcac ctggttgaag
     8641 gacaggccga aggggttgac ccagaaggtg tcgaggtcct ccgggtcgcg cacggagatg
     8701 tgcccggaga tgccctccgc gaagcccgcc ttgccgaaca gccgcatggc cgccgcgagc
     8761 cgctgcttgc ggtggcggcg ctcctcgtcc ggcgaggtga actcgcggtc ggcggggatc
     8821 ggcagcccgg tgtgggtgtc ggcgagatgg ttcgccaaag tgtccgttga cacgggtcga
     8881 gtctcctgat gaatgtcggg gaatggcccg ccacggcatg gtcgtgaacg ccttcggggg
     8941 cacttcggag gagtggcccg gaatggatgt gggggctgcc gggcccggag cggatcccgc
     9001 ctcgggggcg tccgctcctg ctcacgcatc ttgtcccggc ggcgcggcgg aattcagcct
     9061 tcgatggctc tttcgcgctg cacgatgcgg agtttcacag atgcgcaggg gctctattgc
     9121 ggtgcatacg tgggcaaatc gccccgggga aggtcccttg tcggccacct ggggcccggg
     9181 gcgcacgccc cgggcccgga atcagcgcgc tccggccgtc acaccctgcg cgcgtggaag
     9241 atgccccact tcagcagccc cttcgcgcac gccgccgcca cctgcgggag gctctcgcgc
     9301 aggccctcca agtacgccgc gctgaccgac gccacaagag tcggggacgg gtcgcggacc
     9361 tcgtcggtga ggcgttcgta gtgggtctgg aggtgttcgc cgaggtcctc gaagctcacg
     9421 tcggagagcg gcagcgaccg cagctgctcg tcgtagaagg cgggcgtcgc gaagcccgtg
     9481 accgtcagcc ggtccgtgag cgggcgcagt tcgtcggcgg gggcgtcctc gacggccatg
     9541 aagtcggtga ggaccaggtg cccgccgggc ttgaggacgc gcaccgcctc gcgcaggacc
     9601 gtcgcccggt cgggactgtg gcacagcgcg tcctgggacc agaccacgtc gtacgcgccg
     9661 tccggcgcgg gcacgtcctg gaaggagccg gtcaccacgt cgatcagccc gtcgaggccg
     9721 cgctcggcgt tgatctcgcg gtgccggcgg ttctgcacct cgctgatgtt caggccggtg
     9781 acgcgacagc cgaaccgctc ggccaactgc cgtgccgcgc cgccgtaccc ggaccccagg
     9841 tccaggatcg cgcgccccgg cccgagcgcg tccgcggcct tcgccgccat ccgctcgacg
     9901 gtgcgccggg ccgcgacacc gatcgcttcg cgctcgtcgg tgtagatccc gacgtggatg
     9961 ttctccccgc cccacgcctg cgcgtagaag ccgtcgacat cgccggtgtc gtagtacgta
    10021 cgtgccacgt tgtcctgggt catggcacat acctgggcac cgggcggcgg acgcaaaccc
    10081 cgcgctagag aaccggcgga gccgtcgccg cgcccggcag ccgtacggac gccaccgcgc
    10141 cgccgtgctc cgccggggcg agggagacct cgccgcccgc ctggcggatc gtgcgggcca
    10201 cgatggacag gcccaggccg ctgcccggga ggctgcgggc cgagggggag cgccagaatc
    10261 ggtcgaagac gtgggggagt tcgtcggcgg ggatgccggg gccgtggtcg cgcacggtca
    10321 gctcgccgcc ggtcagggtg acctccaccg tgccgccggg cgggctgaac ttcaccgcgt
    10381 tgtccaggag gttgaccagg gcgcgctcca gggcagccgg ttcggcgcgt acgaacca
//
