LOCUS       NZ_CP031455            10399 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Streptomyces olivoreticuli subsp. olivoreticuli strain ATCC 31159
            chromosome, complete genome.
ACCESSION   NZ_CP031455
VERSION     NZ_CP031455.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces olivoreticuli subsp. olivoreticuli
  ORGANISM  Streptomyces olivoreticuli subsp. olivoreticuli
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8809793)
  AUTHORS   Zhang,H.
  TITLE     Complete genome sequence of Streptomyces olivoreticuli ATCC 31159,
            producer of anticancer bestatin and diverse secondary metabolites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8809793)
  AUTHORS   Zhang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2018) Tianjin Key Laboratory of Food
            Biotechnology, College of Biotechnology and Food Science, Tianjin
            University of Commerce, NO.409 Guangrong Road, Tianjin, Tianjin
            300134, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-JUN-2018
            Assembly Method                   :: SMRT Link v. 5.1.0
            Assembly Name                     :: TJCU_BT_311
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: SMRT Link v5.1.0
            Genome Coverage                   :: 145.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003391135.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 04:49:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,562
            CDSs (total)                      :: 7,459
            Genes (coding)                    :: 7,276
            CDSs (with protein)               :: 7,276
            Genes (RNA)                       :: 103
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 61 of 183
            Pseudo Genes (incomplete)         :: 150 of 183
            Pseudo Genes (internal stop)      :: 18 of 183
            Pseudo Genes (multiple problems)  :: 41 of 183
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:12:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3007844
            Orig. end                         :: 3018243
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031455.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Hongyu Zhang.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="25"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(545..1468)
                     /locus_tag="DXZ73_RS12720"
     CDS             complement(545..1468)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019885267.1"
                     /locus_tag="DXZ73_RS12720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SCO1860 family LAETG-anchored protein"
                     /protein_id="WP_116215585.1"
                     /transl_table=11
                     /translation="MPVRRLAALAAAAAATATAVAGPAYATGGGSGDRGKSGAAVLRTG
                     LDVALLNKTVHVPLNASLNEVRAPADADRTALTVTLDGVEQGKPVDVLRADAATARATT
                     DRNKAEGYANLVHARVHVPGLPFLSLIEVQQVTSTAVCEAGRKPTATSNLLGSVSVLGR
                     KVTLTTGGTTKVEVPGVGEVRLDLSRTATTSRTAAASALALRVAVNPLKLNVAEVSGEV
                     TLAEATCEAPAAGEASGTSGGGGGDVKPQTAARASAEPVAAKHAENLAETGGSSATPYV
                     AGAAAALVVAGGGAVVVARRRAAARR"
     misc_feature    complement(1645..1660)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.42"
                     /tool="antismash"
     gene            1741..2715
                     /locus_tag="DXZ73_RS12725"
     CDS             1741..2715
                     /GO_function="GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986841.1"
                     /locus_tag="DXZ73_RS12725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="WP_116211105.1"
                     /transl_table=11
                     /translation="MSTERISPKPVAAQGGPTADRTARLAVTVFPLLVLAAGGVGLSAP
                     AHFTGWAPAVPYLLGVVMFTMGLTLTPPDFRTVVKRPWAVGLGLAAHYVIMPGLGWLIA
                     TVLDLPPQLAAGVILVGCAPSGTASNVVTYLARGDVALSVSVATVSTLVAPLVTPPLTL
                     LLADRFLDVDAGAMVGDILRTVLVPVLGGALVRAVAGRYVDRALRALPWVSALTISVIV
                     LVVVSGSAARIKDAAGLVLLAVVLHNALGLALGYASGRLARLGPPASRAMAFEVGMQNS
                     GLAASLATAHFSPAAALPAAVFSVWHNVSGAAVAAWMSRSRRA"
     misc_feature    2724..2739
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.11"
                     /tool="antismash"
     misc_feature    complement(2740..2754)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.9"
                     /tool="antismash"
     gene            3008..4048
                     /locus_tag="DXZ73_RS12730"
     CDS             3008..4048
                     /NRPS_PKS="Domain: Aminotran_5 (46-342). E-value: 5.4e-23.
                     Score: 73.4. Matches aSDomain:
                     nrpspksdomains_DXZ73_RS12730_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718911.1"
                     /locus_tag="DXZ73_RS12730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_116211106.1"
                     /sec_met_domain="Aminotran_5 (E-value: 8.3e-17, bitscore:
                     59.9, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSLAPDHFSPETVYLNTASWGLIPTGAVAVMTASVAAASVGRPQ
                     EEVFSAAVEAARAAYGRLLGVPAGRVATGSSVAAYCGLIAQSLPSGSEVLVADGDFSSL
                     VNPFHVRHDLRVRTVPLEKVADEVRPGTALVAVSAVQSADGRIAALDEIGAAARAHGAR
                     TLIDVSQAVGWLPVDATAHDYTVCVGYKWLMCPRGTAFLTVPEDLGGLTPVFAGWTSGE
                     EPWESCYGRVEPLAASARRFDVNVPVFSYVAARESLGLVEALGVERIGAYDVALADRFR
                     AGVAGLGHVPVEAAGSAIVAVPGLGDAAARLAAAGVHVSARAGNLRAAFHLYNTVADVD
                     RLLEAL"
     aSDomain        3146..4033
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DXZ73_RS12730_Aminotran_5.1"
                     /evalue="5.40E-23"
                     /label="DXZ73_RS12730_Aminotran_5.1"
                     /locus_tag="DXZ73_RS12730"
                     /protein_end="342"
                     /protein_start="46"
                     /score="73.4"
                     /tool="antismash"
                     /translation="EVFSAAVEAARAAYGRLLGVPAGRVATGSSVAAYCGLIAQSLPSG
                     SEVLVADGDFSSLVNPFHVRHDLRVRTVPLEKVADEVRPGTALVAVSAVQSADGRIAAL
                     DEIGAAARAHGARTLIDVSQAVGWLPVDATAHDYTVCVGYKWLMCPRGTAFLTVPEDLG
                     GLTPVFAGWTSGEEPWESCYGRVEPLAASARRFDVNVPVFSYVAARESLGLVEALGVER
                     IGAYDVALADRFRAGVAGLGHVPVEAAGSAIVAVPGLGDAAARLAAAGVHVSARAGNLR
                     AAFHLYNTVADVDRL"
     gene            complement(4107..4997)
                     /gene="thpD"
                     /locus_tag="DXZ73_RS12735"
     CDS             complement(4107..4997)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013004613.1"
                     /locus_tag="DXZ73_RS12735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /protein_id="WP_116211107.1"
                     /sec_met_domain="PhyH (E-value: 1.2e-37, bitscore: 129.0,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTATATDLYPTRGAREVSVPRQDPVTWSAPGTPGPIGAAELEGYD
                     RDGFLTVDQLLGPYEVAHYRAELDRLIADPAVRADERSVVEPKSRDVRSVFEVHRISEV
                     FAALVRDPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPRMR
                     TVSVSIALTENHDTNGGLMIMPGSHRTFLGCAGATPRDNYKRSLRTQDAGTPSDEALTG
                     LAERHGIKLFTGPAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENAAVEPFAAPV
                     RRPEFVGARDFTPVV"
     gene            complement(5001..5399)
                     /locus_tag="DXZ73_RS12740"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123624.1"
                     /locus_tag="DXZ73_RS12740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_116211108.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.3e-53, bitscore:
                     179.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVRARSGTWESKRIVLAEERVGFSLHETTLY
                     AGTETSMWYANHIEAVLCVEGEAELTNDETGEKHRITPGTMYLLDGHEKHTMRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEEG"
     gene            complement(5440..6744)
                     /gene="ectB"
                     /locus_tag="DXZ73_RS12745"
     CDS             complement(5440..6744)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-364). E-value: 2.2e-70.
                     Score: 229.2. Matches aSDomain:
                     nrpspksdomains_DXZ73_RS12745_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="DXZ73_RS12745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_116211109.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1e-73, bitscore:
                     247.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRSWPAVFDRAHGSRMYDEDGHTY
                     LDFFAGAGALNYGHNNPVLKRALLDHLERDGVTHGLDMATTAKRVFLETFQDVVLRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKAKGREPVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFEGRVPDFLWFERLLEDQGSGLDTPAAVIVETVQGEGGVNVARP
                     EWLRALAALCERRDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTLSKSIGGYGMPMS
                     LTLFKPELDLWEPGEHNGTFRGNNPSFVTAAAALNAYWTDGQMEKQTLARGEQVAQALR
                     AIGAEHPELGARYRGRGLVWGLEFRGSGGRSPEGRSADRARASAVAARAFELGLLIETS
                     GPESEVVKLLPALTITPEDLDEGLRILARAVRETA"
     aSDomain        complement(5653..6660)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DXZ73_RS12745_Aminotran_3.1"
                     /evalue="2.20E-70"
                     /label="DXZ73_RS12745_Aminotran_3.1"
                     /locus_tag="DXZ73_RS12745"
                     /protein_end="364"
                     /protein_start="28"
                     /score="229.2"
                     /tool="antismash"
                     /translation="FDRAHGSRMYDEDGHTYLDFFAGAGALNYGHNNPVLKRALLDHLE
                     RDGVTHGLDMATTAKRVFLETFQDVVLRPRDLPYKVMFPGPTGTNAVEAALKLARKAKG
                     REPVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFEGRVPDFLWFER
                     LLEDQGSGLDTPAAVIVETVQGEGGVNVARPEWLRALAALCERRDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTLSKSIGGYGMPMSLTLFKPELDLWEPGEHNGTFRGNNPSFV
                     TAAAALNAYWTDGQMEKQTLARGEQVAQALRAIGAEHPELGARYRGRGLVWGLEF"
     gene            complement(6789..7337)
                     /gene="ectA"
                     /locus_tag="DXZ73_RS12750"
     CDS             complement(6789..7337)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607778.1"
                     /locus_tag="DXZ73_RS12750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_116211110.1"
                     /transl_table=11
                     /translation="MTAAQADLVRARSEFPEMPEGLTLDSPRVEDGAAIWRIARDSEVL
                     DLNSSYSYLLWCRDFAATSAVARGADGEPAGFVTGYLRPGRPCTLVVWQVAVDHAHRGR
                     GLAAALLDGLVARTARDLGVRGVETTVTPDNTASNRLFASFAERHGAPVEREVLFAGGL
                     FPDAGHAPEVLHRIGPLAA"
     misc_feature    complement(7362..7371)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: medium, score: 16.86"
                     /tool="antismash"
     misc_feature    complement(7470..7484)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.51"
                     /tool="antismash"
     misc_feature    complement(7494..7509)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     gene            7737..8837
                     /locus_tag="DXZ73_RS12755"
     CDS             7737..8837
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606343.1"
                     /locus_tag="DXZ73_RS12755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="amidohydrolase family protein"
                     /protein_id="WP_205519161.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 4.1e-14, bitscore:
                     51.3, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDSEVLHIKGRVLTGPDPDHGTRDELWAVDGRITFDRPTGVRDV
                     TTVEGWVLPGLVDAHCHVGLDAHGPVDESTSEKQALTDRDAGALLLRDAGSPSDTRWVD
                     DREDLPRIIRAGRHIARTRRYIRNYAHEIEPEDLVAYVAREARRGDGWVKLVGDWIDRE
                     TGDLSLCWPRGAVEAAIAEAHRLGARVTAHCFAEESLAPLVEAGIDCVEHATGLTEETI
                     PLFAERGVAIVPTLVNIATFPGLAQGGEAKFPRWADHMRRLHARRYETVGAAYDAGIPV
                     YVGTDAGGSLAHGLVAQEVAELVKAGIPATAALAAASWGAREWLGRPGLEEGAPADLVV
                     YESDPREDVRVLGAPRRVVLRGKVVR"
     gene            complement(9103..10257)
                     /locus_tag="DXZ73_RS12760"
     CDS             complement(9103..10257)
                     /EC_number="3.1.1.103"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019060324.1"
                     /locus_tag="DXZ73_RS12760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="WP_116211112.1"
                     /transl_table=11
                     /translation="MSEKAIARGTVAKGYEAVREEFEATLAEERAGHSAQLAAYVDGRQ
                     VVDLWGGPGIEADSLTGVFSSTKGAAHLAVALLVQDGTLDLDREVSHYWPEFAAEGKEH
                     ITLRDLLAHRAGLPGVEGGLAPDELADDRVIAERLAAQRPLWRPGSAFGYHALVIGALT
                     GEVVRRVTGLTMQEFYETRIRAPYGIDFYMGLPEDQEHRVLHTQPMDPTPEQKQALAQG
                     HSGPESLQGIAFNINHPVPTVLEELPNSRAVRAAGPASVGGMASARGLARLYAAAISHV
                     DGKEPLLTPGTAADFAQIHSVGYDVVLRQHMTFGLGFSPVSDAYPFLGGRSFGHGGAAG
                     SHAFADPGRALAFGYNRRRFAFPGGPAPEVARLAQAVYDCASAR"
ORIGIN
        1 cgagcgcgag tccctggcgt cccgtacgga cctgccgggc ctggtggtgc tgcgcagcct
       61 cacgaagacc tggggtctcg cggggctgcg catcggctac gtcctcgcct cgcccggtgc
      121 gatcgcgaag ctggaacggg cccagccgct gtggcccgtc tcctcccccg ccctcgcggc
      181 ggcggaggtg tgcagcgctc ccccggccct cgccgaggcc gcccacgcgg cacaccattt
      241 ggcgtcggac cgtgcccatc tggtcgcggc cctcgcggag ttcgaggggg tgcgcgtgca
      301 cggcccggcg gcgggcccct tcgtcctgat acacctgccg gaagcggtga cggtccgctc
      361 ccgcctgcgc ggcctgggct tcgcggtccg ccggggcgac acgttccccg gcctgggccc
      421 ggactacctc cgcctggcgg tccgcgaccg cgcgacgacg gaccgcttcg tggacgcgct
      481 gcgatcggta ctcacggagg gctgaaattc cagcccagtt acgggaaggg gcggggtggg
      541 gaaactagcg ccgggcagcc gcacgacgac gggccacaac caccgctccc ccacccgcca
      601 cgacaagcgc cgcggccgcc cccgccacat acggcgtggc cgagctgcct cccgtctcgg
      661 ccagattctc cgcgtgcttc gcggcaaccg gctccgcgga cgcacgggcc gccgtctgcg
      721 gcttgacatc accaccgccc ccacccgacg tgccggaagc ctccccggcc gcaggggcct
      781 cgcatgtcgc ctccgccagg gtcacctcgc ccgagacctc cgcgacgttc agcttcagcg
      841 gattgacggc gacgcgcaac gcgagcgcgc tggcggcggc cgtccgcgag gtggtcgccg
      901 tccgggacag atccagccgc acctcgccga cgccgggcac ctcgaccttc gtcgtgccgc
      961 ccgtggtgag cgtgaccttc ctgccgagca ccgagacgct gcccaggagg ttcgaggtgg
     1021 cggtgggctt ccggccggcc tcgcacacgg ccgtcgacgt gacctgctgg acctcgatga
     1081 gcgagaggaa cggcagtccg gggacgtgca cccgggcgtg gacgaggttg gcgtagccct
     1141 ccgccttgtt ccggtcggtg gtggcgcggg cggtggcggc gtcggcgcgc aggacgtcga
     1201 cgggcttgcc ctgttcgacg ccgtcgagcg tgacggtcag ggcggtcctg tcggcgtcgg
     1261 ccggtgcgcg cacctcgttc agggaggcgt tgagcgggac gtggacggtc ttgttgagca
     1321 gggcgacgtc gagtccggtg cggaggacgg ccgcgccgga cttccctcgg tcgccggagc
     1381 cgcctccagt ggcatacgcg ggcccggcga cggcggtcgc ggtcgcggcg gccgccgccg
     1441 cgagcgcggc gagacggcgt acgggcatgc cgaaggaagg gctggtggtg ctgtacacgt
     1501 gtgtggaacc cccacaagag gcttggtgcc gtcggcgcgg tcgatggggg acagcgccgc
     1561 gccgacggcg ggaccccgcc atatttgacc cctggagagt gaacgggcag tctcctgagg
     1621 ccagttcacc ccaacgagta agtttcgcag ccgcattcga ttcgtccggc gcgtgagtcg
     1681 accctcgtca cccgcgttac ccctgttcgg cggaggcggt gatcgctaag gtgccgccgt
     1741 atgtcaaccg agcggatatc cccgaagccc gtcgcagctc agggcggccc caccgccgac
     1801 cggacggccc ggctggccgt caccgtcttc ccgctgctgg tgctcgcggc gggcggcgtg
     1861 gggctctcgg cccccgcgca cttcaccggc tgggccccgg cggtgcccta tctgctgggc
     1921 gtggtgatgt tcaccatggg cctcaccctg acgcccccgg acttccgtac cgtcgtcaag
     1981 cggccctggg cggtggggct gggtctggcc gcccactatg tgatcatgcc ggggctgggc
     2041 tggctgatcg ccaccgtgct cgacctgccg ccgcaactgg ccgccggggt catcctggtg
     2101 ggctgcgcgc ccagcggtac ggcctccaat gtggtcacct acctggcgcg gggcgacgtc
     2161 gcgctgtccg tctcggtcgc gacggtgtcc acgctggtcg ccccgctggt gacacccccg
     2221 ctgacgctgc tgctcgccga ccgcttcctg gacgtggacg cgggggcgat ggtcggtgac
     2281 atcctcagga cggtgctcgt cccggtcctc ggcggggcgc tcgtccgcgc cgtcgccggg
     2341 cggtacgtcg accgggcgct gcgcgccctg ccgtgggtct cggcactgac gatctccgtc
     2401 atcgtgctcg tcgtggtgtc cggcagcgcg gcccgcatca aggacgccgc cggcctggtc
     2461 ctcctggcgg tcgtcctgca caacgccctc ggcctggcgc tcggttacgc ctccggccgg
     2521 ctggcccggc tgggcccgcc cgcgagccgg gccatggcct tcgaggtcgg catgcagaac
     2581 tccggcctcg cggcgtccct ggccaccgcg cacttcagcc cggcggcggc cctgcccgcc
     2641 gcggtcttct cggtctggca caacgtctcg ggcgcggcgg tcgcggcgtg gatgtcccgc
     2701 tcgcggcgcg cttgacggcg gcggtcacgt ggtcgtccct ggagatgcgg ttcatacgac
     2761 cagggtcccg ggggcgggcg cccggcgcgt cggccccagc ggcggatcct ctccgccgca
     2821 gggctgatcc ttttctcgtc ctcgaggcag aacgaggcgc gcggacctcg cccttgagga
     2881 ggacgggcgg tacggctccg ccccgagccc gcggggcgcc ccggccacag gcggcgacgg
     2941 acccgcaccc ggcgaagcca ccgcaagccg tggacggatc gatcaccccc acccatagtg
     3001 gacaaccatg aagagcctcg cacctgatca cttcagtccc gagaccgtct acctcaacac
     3061 cgcctcctgg ggcctgatcc ccacgggcgc ggtcgcggtc atgaccgcgt ccgtcgccgc
     3121 cgcgtccgtg gggcgcccgc aggaggaggt gttctcggcg gcggtggagg cggcgcgcgc
     3181 ggcgtacggg cggttgctcg gcgtgcccgc cgggcgggtg gccacgggga gttcggtggc
     3241 ggcctactgc gggctgatcg cccagtccct gccgtccggg tccgaagtcc tcgtcgccga
     3301 cggcgatttc agctcgctcg tcaacccctt ccatgtgcgc cacgacctgc gggtgcgcac
     3361 cgtccccctg gagaaggtcg ccgacgaggt gcggccgggc acggccctgg tcgcggtgag
     3421 cgccgtccag tcggcggacg gccggatcgc cgcgctcgac gagatcggcg ccgccgcccg
     3481 cgcccacggc gcccgtacgc tgatcgacgt cagccaggcc gtcggctggc tgcccgtcga
     3541 cgcgaccgcc catgactaca cggtctgcgt ggggtacaag tggctgatgt gcccccgtgg
     3601 caccgccttc ctgaccgtgc ccgaggatct cggcggtctc acaccggtgt tcgcgggctg
     3661 gacctcgggc gaggaaccgt gggagagctg ttacgggcgg gtcgagccgc tcgccgcgtc
     3721 cgcgcgccgc ttcgatgtga acgtgcccgt cttctcctac gtggccgccc gcgagagcct
     3781 cggcctggtg gaggcgctcg gcgtcgagcg catcggcgcg tacgacgtgg cgctcgccga
     3841 ccggttccgc gcgggggtcg ccgggctcgg tcacgtaccg gtggaggcgg cgggatccgc
     3901 gatcgtggcg gtcccgggcc tcggggacgc ggcggcgcgg ctcgcggcgg cgggcgtgca
     3961 cgtgagcgcg cgtgcgggca atctccgggc ggcgttccac ctctacaaca cggtcgcgga
     4021 cgtggaccgg ttgctggaag ccctgtagcc cggtcccgcg cggccgggcg ggcgggattc
     4081 cccggcccgc ccggccgcgc ggctgctcac accaccggcg tgaaatccct cgcgcccacg
     4141 aactccggcc tgcgcaccgg tgcggcgaac ggttccaccg ccgcgttctc cacgctgttg
     4201 aagacgatga agacgttgct gcgcgggaag ggcgtgatgt tgtcaccgga accgtgcatg
     4261 cagttgcagt cgaaccatgt cgccgacccg gccggcccgg tgaacagctt gatgccgtgc
     4321 cgctccgcga ggccggtcag ggcctcgtcg gacggtgtgc ccgcgtcctg cgtgcgcagc
     4381 gaccgcttgt agttgtcgcg cggggtcgca ccggcgcagc cgaggaacgt ccggtgcgag
     4441 ccgggcatga tcatcagacc gccgttggtg tcgtggttct cggtgagggc gatggagacc
     4501 gacacggtcc gcatgcgcgg gagcccgtcc tcggcgtgcc aggtctcgaa gtcggagtgc
     4561 cagtagaacc ccgaggcacc gaagccgggc ttgacgttga tcctggactg gtggacgtag
     4621 acgtccgagc cgaggatctg ccgggcccgg cccaccaccc gggggtcgcg cacgagcgcg
     4681 gcgaagacct cgctgatgcg gtggacctcg aagaccgacc gtacgtcccg cgacttcggc
     4741 tcgacgacgg agcgctcgtc ggcgcgtacg gccgggtcgg cgatcagccg gtcgagttcg
     4801 gcgcggtagt gggccacctc gtacgggccg aggagttgat ccaccgtcag gaaaccgtcg
     4861 cggtcgtagc cctccagctc ggccgcgccg atcgggcccg gggtgccggg ggcggaccag
     4921 gtgacggggt cctggcgggg gacggacacc tctcgggcgc cgcgggtggg gtagaggtct
     4981 gtggccgtgg cggtcatcgg tcagccctcc tcggtgagca gggggtagac gccgttctcg
     5041 tcgtggtcct cccgtccggt gaccggcggg ttgaagacgc agacgcagcg gaagtcggtc
     5101 ttcggccgca tggtgtgctt ctcgtggccg tccagaaggt acatcgtgcc gggcgtgatc
     5161 cggtgcttct cgccggtctc gtcattggtg agttcggcct cgccctccac gcacaggacg
     5221 gcctcgatgt ggttggcgta ccacatggag gtctcggtcc cggcgtagag cgtggtctcg
     5281 tgcagggaga agccgacgcg ttcctcggcg aggacgatgc gcttgctctc ccaggtgccg
     5341 gacctggccc tgacgtgccg gtcggtgccc tcgatgtcct tgaacgagcg gacgatcacg
     5401 gtgctgcctc tttctgtcgg gggtgcgttg ccggtcgggt caggcggtct cgcggaccgc
     5461 gcgggcgagg atccgcaggc cctcgtcgag gtcctcgggg gtgatggtca gggccgggag
     5521 cagcttgacg acctcgctct cggggccgga ggtctcgatg agcaggccga gttcgaaggc
     5581 ccgcgcggcg acggcggagg cccgcgcccg gtcggcgctg cgtccttccg gggagcgacc
     5641 cccggacccc cggaactcaa gaccccagac gagaccccgg ccgcggtacc gcgcgcccag
     5701 ctcggggtgt tcggcgccga tcgcccggag ggcctgcgcg acctgttcgc cgcgcgcgag
     5761 ggtctgcttc tccatctggc cgtcggtcca gtaggcgttc agggccgcgg cggcggtgac
     5821 gaaggagggg ttgttgccgc ggaaggtgcc gttgtgctcg ccgggctccc agaggtcgag
     5881 ctcgggcttg aagagggtga gcgacatcgg catcccgtag ccgccgatgg acttcgagag
     5941 ggtgacgatg tccggggtga tgcccgcctc ctcgaaggag aagaaggcgc cggtgcggcc
     6001 gcagcccatc tggatgtcgt cgacgatcag cagcatgtcg cggcgctcgc agagcgcggc
     6061 gagggcgcgc agccactcgg ggcgggcgac gttgacgccg ccctcgccct gcacggtctc
     6121 gacgatgacg gcggcggggg tgtcgaggcc cgagccctgg tcctccagga gccgctcgaa
     6181 ccacaggaag tccgggacgc gcccctcgaa gtagttgtcg aacggcatgg gcgtgccgtg
     6241 caccagcgga atgccggccc cggcgcgctt gaaggcgttt ccggtgacgg cgagcgagcc
     6301 gagcgacatg ccgtggaagg cgttggtgaa ggagacgacc ggctcacggc ccttggcctt
     6361 gcgggccagt ttgagcgcgg cttccacggc gttggtgccc gtggggccgg ggaacatcac
     6421 cttgtacggg aggtcgcgcg gccggagcac gacgtcctgg aaggtctcca ggaacacccg
     6481 cttggcggtc gtcgccatgt ccaggccgtg ggtgacgccg tcgcgttcca gatggtcgag
     6541 cagggcgcgt ttgagcacgg ggttgttgtg cccgtagttg agcgccccgg cgccggcgaa
     6601 gaagtcgagg taggtgtggc cgtcctcgtc gtacatccgg ctgccgtggg cgcggtcgaa
     6661 gacggcgggc cagctgcggc agtagctgcg cacctccgac tccagggtct cgaagacgct
     6721 cagtgcgggc ggggtgatgg tcacagcaca ctccaggaat gggggtgtcg gggctcggcg
     6781 ggggggggtc aggccgcgag ggggccgatg cggtggagca cctcgggcgc gtgcccggcg
     6841 tcggggaaca ggccgccggc gaagagcacc tcgcgctcca cgggcgcgcc gtgccgctcg
     6901 gcgaaggagg cgaacagccg gttggaggcg gtgttgtcgg gcgtgacggt ggtctcgacc
     6961 ccgcgcacgc cgaggtcccg ggcggtccgg gcgaccaggc cgtcgagcag ggcggcggcc
     7021 aggccccggc cgcggtgcgc gtggtcgacg gcgacctgcc agaccacgag ggtgcagggc
     7081 cggccgggac ggaggtagcc ggtgacgaac ccggccggct ccccgtcggc accgcgggcc
     7141 acggcggagg tggcggcgaa gtcgcggcac cacagcaggt agctgtagga ggagttgagg
     7201 tccagcacct ccgagtcacg ggcgatccgc cagatcgcgg ccccgtcctc gacgcgtggg
     7261 ctgtcgagtg tgagcccctc cggcatttcc gggaattcac tgcgggcacg tacaaggtct
     7321 gcttgtgcgg cggtcatgcg gagtgaattt accgaggcga atcaaaaaat gcatgggggg
     7381 acgaccttta cggtcgcggc ggtgccgtgt tatcgcgcgg gtatgggcgg gcgcgcaagt
     7441 ggacgcctaa atgccctcat ttatccggta tcgacgggcg ttatgggcgg gcttgtggag
     7501 tcggtcacag ggcggggccg cgtgttggga agtcattgga attccccggt ttagggtcgg
     7561 gaagggaggg caggcgaagg cagggaattt gcattcacgc atatctggag ggaatcggaa
     7621 ccggaaaagc cggtgccatg cgaaacgagc ccgtccggaa tccgcggacc ggggtccggg
     7681 gcggcgcccc ggcaacggcg ccgcacccga acacgccccc gaataaagtg gcgcccatga
     7741 gcgacagcga agtgctgcac atcaaggggc gagtgctcac cggtccggac cccgaccacg
     7801 ggacccgcga cgaactgtgg gccgtggacg gccggatcac cttcgaccgc ccgacggggg
     7861 tgcgcgacgt caccaccgtc gagggctggg tcctccccgg tctcgtggac gcccactgcc
     7921 acgtggggct cgacgcccac ggcccggtcg acgaatcgac cagcgagaag caggcgttga
     7981 cggaccgcga cgcgggggcg ctgctgctgc gggacgcggg ttccccgtcg gacacccggt
     8041 gggtcgatga ccgcgaggac ctgccgcgga tcatccgcgc gggccgccac atcgcccgta
     8101 cccgccgcta catccgcaac tacgcgcacg agatcgagcc cgaggacctg gtcgcctatg
     8161 tggcgcgcga ggcgcggcgc ggggacggct gggtcaagct cgtcggcgac tggatcgacc
     8221 gggagacggg cgacctgtcg ctgtgctggc cgcgcggcgc ggtggaggcc gcgatcgcgg
     8281 aggcgcaccg gctgggggcg cgggtcacgg cgcactgctt cgccgaggag tccctcgcgc
     8341 cgctggtcga ggcgggcatc gactgcgtcg agcacgccac cgggctgacc gaggagacga
     8401 tcccgctgtt cgccgagcgg ggcgtggcga tcgtgccgac gctggtcaac atcgcgacgt
     8461 tcccggggct ggcgcagggc ggcgaggcga agttcccgcg ctgggcggac cacatgcgcc
     8521 ggctgcacgc gcggcgctac gagacggtgg gcgcggcgta cgacgcgggc atccccgtct
     8581 acgtcggcac ggacgcgggc ggttcgctcg cgcacggcct ggtggcccag gaggtcgccg
     8641 agctggtcaa ggcgggcatc ccggcgacgg cggcgctcgc ggcggcgtcc tggggcgcgc
     8701 gggagtggct cggccgccct ggcctggagg agggcgcccc ggcggacctg gtggtctacg
     8761 agagcgatcc ccgggaggac gtcagggtgc tgggcgcccc tcgacgggtg gtgctccggg
     8821 ggaaggtggt gcgatagtcc gcgtccggcg gatcattgcc gagtgcgggc cgtcgtaccg
     8881 ccctccgcgg cgggcccctg ctgagggtcc cccggaggtg cccccacggg cttgatccgg
     8941 tgcggatcga cgatcacctg ccggcacgtc cagcccgtcc ggcgactgag gacatcaccc
     9001 tcagcggtgg tgcaggcgac acggcccgcg aaggcccgcg caccggaccc accggacacg
     9061 gcgctcccgg gcgcgtggcg cgcgcccggg agcggcagcg gctcagcgcg cggacgcgca
     9121 gtcgtacacc gcctgggcga ggcgggcgac ctccggggcc gggccgccgg ggaaggcgaa
     9181 gcggcggcgg ttgtagccga aggcgagagc gcggcccggg tcggcgaagg cgtgcgagcc
     9241 ggccgcgccg ccgtgtccga aggaccgtcc gcccaggaac gggtaggcgt ccgagaccgg
     9301 ggagaagccc aggccgaagg tcatgtgctg acgcaggacc acgtcgtatc ccacggagtg
     9361 gatctgggcg aagtcggcgg cggtcccggg cgtcagcagc ggctccttgc cgtcgacgtg
     9421 gctgatggcc gccgcgtaca ggcgggcgag gccgcgggcg gaggccatgc cgccgacgga
     9481 ggcggggccg gcggcgcgga cggcacggga gttgggcagc tcctccagca cggtcggcac
     9541 ggggtggttg atgttgaacg ctatgccctg gaggctctcc ggtccgctgt gcccctgcgc
     9601 cagggcctgc ttctgctcgg gcgtcgggtc catgggctgg gtgtgcagca cccggtgctc
     9661 ctggtcctcg ggcaggccca tgtagaagtc gatgccgtac ggggcgcgga tccgggtctc
     9721 gtagaactcc tgcatggtca gcccggtcac gcgccggacg acctcgccgg tgagggcgcc
     9781 gatgaccagg gcgtggtagc cgaaggcgct gcccgggcgc cagagcgggc gctgcgcggc
     9841 cagccgctcg gcgatcacgc ggtcgtccgc gagctcgtca ggggcgaggc cgccctcgac
     9901 accgggcagg ccggcgcggt gggcgagcag gtcgcgcagc gtgatgtgtt ccttgccctc
     9961 ggcggcgaac tcgggccagt agtggctcac ttcccggtcc aggtcgaggg tgccgtcctg
    10021 gacgagcagc gccacggcga ggtgggccgc gcccttggtg gaggagaaga cgccggtgag
    10081 cgagtcggcc tcgatcccgg gcccgcccca caggtcgacc acctggcggc cgtccacgta
    10141 ggcggcgagc tgggcggagt gccccgcgcg ctcctccgcg agcgtggcct cgaactcctc
    10201 gcgcaccgct tcgtagccct tggctacggt cccccgggcg atggccttct ccgacatgtc
    10261 gcgcgctccc tcgaagctcg atgatcttcc gtcggggagt ggagcgacga gaggcgcccg
    10321 gatattccgt cacccggccc ggggcgcgcg gccacggttt cggggagggg cggggccggg
    10381 gaaggagaac ccatggcca
//
