LOCUS       NZ_CP031455            11191 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Streptomyces olivoreticuli subsp. olivoreticuli strain ATCC 31159
            chromosome, complete genome.
ACCESSION   NZ_CP031455
VERSION     NZ_CP031455.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces olivoreticuli subsp. olivoreticuli
  ORGANISM  Streptomyces olivoreticuli subsp. olivoreticuli
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8809793)
  AUTHORS   Zhang,H.
  TITLE     Complete genome sequence of Streptomyces olivoreticuli ATCC 31159,
            producer of anticancer bestatin and diverse secondary metabolites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8809793)
  AUTHORS   Zhang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2018) Tianjin Key Laboratory of Food
            Biotechnology, College of Biotechnology and Food Science, Tianjin
            University of Commerce, NO.409 Guangrong Road, Tianjin, Tianjin
            300134, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-JUN-2018
            Assembly Method                   :: SMRT Link v. 5.1.0
            Assembly Name                     :: TJCU_BT_311
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: SMRT Link v5.1.0
            Genome Coverage                   :: 145.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003391135.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 04:49:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,562
            CDSs (total)                      :: 7,459
            Genes (coding)                    :: 7,276
            CDSs (with protein)               :: 7,276
            Genes (RNA)                       :: 103
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 61 of 183
            Pseudo Genes (incomplete)         :: 150 of 183
            Pseudo Genes (internal stop)      :: 18 of 183
            Pseudo Genes (multiple problems)  :: 41 of 183
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:12:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 470514
            Orig. end                         :: 481705
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031455.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Hongyu Zhang.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11191
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11191
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11191
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6191](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6191
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(24..36)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 22.57"
                     /tool="antismash"
     gene            316..954
                     /locus_tag="DXZ73_RS02150"
     CDS             316..954
                     /EC_number="3.1.3.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1101: phosphoglycerate mutase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /locus_tag="DXZ73_RS02150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="histidine phosphatase family protein"
                     /protein_id="WP_116209347.1"
                     /transl_table=11
                     /translation="MTGSVVGSVTITTVRHSLTAHNTAGIITGRLNEPLSAEGRAAASG
                     FARAHGTLSAEVVLSSPARRAIDTARLVTGLPEEDIVLDARCHERHYGALQGLDREQVS
                     AYVARIVYVEAGGTRHSVNPPGGETLTELRARAHRFLMAVLSLSTTSVLVSSHGTFLQQ
                     FHGLLLERGTMETLACHIRSLQIDRFTLRGEGRPRHQLIHPGLSTGLVW"
     gene            1158..1790
                     /locus_tag="DXZ73_RS02155"
     CDS             1158..1790
                     /GO_function="GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1228: methionyl-tRNA formyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /locus_tag="DXZ73_RS02155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="formyltransferase family protein"
                     /protein_id="WP_162889968.1"
                     /transl_table=11
                     /translation="MLDRLDYPPGELMSFHHLAGNLGVPFHEIENINTGHGSRVAREFQ
                     PDLILSVKFGQIFKEPILSLPSFGILNTHSGALPQYAGLWAPVRTLLAGERRFTSTLHV
                     VDQGIDTGPVIASGHLPVEPNRSLFWHLPRLYVLGIELFLQVLPGLTKCTRITAHRQEN
                     SRRRYYGKLTTEEEAAFEKQGFRFLDDRDYAEITRRFGVDLFTVSSE"
     gene            2010..2354
                     /locus_tag="DXZ73_RS02160"
     CDS             2010..2354
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /locus_tag="DXZ73_RS02160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transposase"
                     /protein_id="WP_162889969.1"
                     /transl_table=11
                     /translation="MKAVRRVTVTQAAPALVALPGVGPETAGQLLETAGDNPDRLVSGA
                     AFTHPCATAPVSASSGRTDRHRLNHGSNRHPNSALHTIVLVRMSFDHCTREYVARRTTQ
                     GLTKKESCDA"
     gene            complement(2380..3495)
                     /locus_tag="DXZ73_RS02165"
     CDS             complement(2380..3495)
                     /EC_number="2.4.-.-"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /locus_tag="DXZ73_RS02165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="WP_116209350.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 2.3e-14,
                     bitscore: 52.2, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRIAYLHGGSIPSFFANGVHVMRMCDAFTAAGHDVTLYALPGSWP
                     ANDPYAYYGVRHRFPIRTVPAPDYTPAGYWKRAERIRDLLIRDTVPDLVYGREPYSLTV
                     ASDLAPFVYEAHQIRRDLPPGTEHALLGLPRLARLVVITHALARDLRSTHGDLGALPIL
                     VAPDCADPPPPAGAAAAPRLPGRPDVPRIGYVGHLYAGRGIGLILDLADRLPGLEFHLV
                     GGTSEDRAHWEGSRPLPNAHFHGHRPPAALPAYFPLFDVALAPHQRKVYTWGKLDEIGR
                     WTSPMKLFEYMSHGRPIIASDLPVLREVLQDRVNCLLRPPDEPDAWADALTELVGDETL
                     RRTLGDEARRQFLERYTWRRRADLVLEGLGA"
     misc_feature    3717..3733
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.32"
                     /tool="antismash"
     misc_feature    3773..3789
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.73"
                     /tool="antismash"
     gene            4093..5001
                     /locus_tag="DXZ73_RS02170"
     CDS             4093..5001
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04267.2"
                     /locus_tag="DXZ73_RS02170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HEXXH motif-containing putative peptide
                     modification protein"
                     /protein_id="WP_162889970.1"
                     /transl_table=11
                     /translation="MTRSALTRAELTVTQKESAHPAVVETAHAAQRVLRAGPDRERLDG
                     LRRMLDRARAGIAAAPPSVLPWPVDLAAPHPHLERSVTRALESVPKRDGDFESPHGSAV
                     VAWRERDISTLRETIALLAAAWPEAHAELRETLVQIALLDGPAIDGFTDFVVHGAVFIR
                     RDRLETGRDALPGPVRLAEALVHEGAHTRCNAASVAGEPFLRPARGSEPLVTTPLRIDP
                     RPLNGLFQQVVVLARSLLLYRRMLDTDATAAASAAVRARHDHVARAAAQGVRALSEHRG
                     ALTDRGASVLEDAAGLPRVRA"
     gene            5001..6191
                     /locus_tag="DXZ73_RS02175"
     CDS             5001..6191
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /locus_tag="DXZ73_RS02175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YcaO-like family protein"
                     /protein_id="WP_116209352.1"
                     /sec_met_domain="YcaO (E-value: 1.2e-27, bitscore: 96.3,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTPRWEPQVFAPYAPWPEVVFARVAARSPQFDGVCAAGDQPVVV
                     GSAAGHRTEHVVASARGELLERVGNILAGRAAEANATVVAAHGELRRKGIPALDPAELA
                     PADSPDTVRSARRLWVLGRWARTGDETFVPAGAVFLHHRAPVGCAPGPGAGSTGVASHP
                     DPRSAADHAAWETLERDLVRRSWYGISPAPTALPAAAIGLPAPLAKLLDGIGTTATALC
                     LPSPGGSRCVVVCLHAPDRTRQAFGARCGPDDGDLAPLVEKAAYEALMVRWSVHTPVAR
                     GTWARWHGRGSPDSAVQHALWAYHRQDSLGLWTARAPAHGRRPAATAGGEPVRSGIAAL
                     SAHTGRDLVLVDTTCGPARDAGATVVRVVAPGARALPTGPDGERGLPVPPGPHPFG"
     gene            6281..7096
                     /locus_tag="DXZ73_RS02180"
     CDS             6281..7096
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1248: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /locus_tag="DXZ73_RS02180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_116209353.1"
                     /transl_table=11
                     /translation="MPTSQDLTAPATATNPDAAHTGNGHPAPAACGPGYADAHAADYDR
                     WFSKPGITGATVDTLANLAGDAPVLELGVGTGRIALPLAERGLDVHGVEASHAMIRRLR
                     EKPGGTRIKVTMGDFADVPLTGTFSLVYAAGGTFFELADREAKQRCLAAVADRLAPGGA
                     LVLDSHVPEALAVAAASGVPEVVSETDEHLVLCHRRIDPSTQTYRSHYVIHETDRTRHL
                     RIHFHYVSPGELDLMAERAGLRLIQRQGNWAGGPFTKDSTYHVSVYGPR"
     gene            7148..8341
                     /locus_tag="DXZ73_RS02185"
     CDS             7148..8341
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /locus_tag="DXZ73_RS02185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cytochrome P450"
                     /protein_id="WP_116209354.1"
                     /sec_met_domain="p450 (E-value: 7.3e-18, bitscore: 63.3,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDTDPGPTTGFPLPHRDGLGPLPEFTALRRDAPCARIRLPSGDP
                     AWLVTRYADVRTVLGDPRFSRTRATRGTGPRMASVPTLPDSILAADPPEHTRLRKLIAP
                     AFTARRAQAMRRDMARLVSGLLDALTAHDRPADLVPLFTRPLPLAVICDLLGTPRQDGD
                     RLDAWCDTLRNLTAAPDAAVTAAVDEMTGYLAALVAAKRRHPADDVVSTLIAAHDDGER
                     LTQEELISFCLVLLVGGYGTTADRLAGMVHLLLDEPGRYRRLCRAPDTIPRAAEELLRY
                     AQTNVQANLRVATEDVELGGVTIAEGEAVMAVNSSANHDETVFEDPGRLDLDRAHNPHL
                     SFGYGVHHCVGAQIARVQLQEALAGLVHRLPDLRAAAPPRWKTGLKTRAPRTLPVTW"
     gene            8349..8621
                     /locus_tag="DXZ73_RS02190"
     CDS             8349..8621
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018540990.1"
                     /locus_tag="DXZ73_RS02190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ferredoxin"
                     /protein_id="WP_116209355.1"
                     /transl_table=11
                     /translation="MPSGDDPATASDGTLRAHIDPELCVGSGICLATAPRHFEPTMDAR
                     SRARPDAPPADDRAQDAAALCPMEAIHFVRAREPTPRTAPPEARS"
     gene            8868..9809
                     /locus_tag="DXZ73_RS02200"
     CDS             8868..9809
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /locus_tag="DXZ73_RS02200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_275898610.1"
                     /transl_table=11
                     /translation="MCGAASTFFMPASSDILTDIVPAETRSAANALLRFGQTAVKVGGP
                     AAGAALVAVVGPPWGIAWDALSFAGSAVLLSRLTVRGATVTRQPEFRHQLRDGWAGFRS
                     RRRLAIMTRQAALAVPVRMAGHQLLGPLYGQRVLGGASAWGTVMSAFAAGLVSGADAAL
                     WWRPRGVGIVGCAGTGAMAAPPATMALHTGATGLVVTTASTGAAVSLSMTGWLQEVIPL
                     DRVTADTAFGQSVTLPLDYLAASPAAHVLGLGAALIAVLVVLLPEVRLLGRPVIVSGRG
                     GAGRRPPRRPRAATAVRGSDAWRRSRSRSART"
     gene            complement(9691..10536)
                     /locus_tag="DXZ73_RS02205"
     CDS             complement(9691..10536)
                     /GO_function="GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (extras)
                     Sulfotransfer_1: Sulfotransferase domain"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /locus_tag="DXZ73_RS02205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="WP_162889971.1"
                     /transl_table=11
                     /translation="MVLPTPSGGPAKPADAEGDVYVISFPKCGRTWLRVLMAKAISTAR
                     GIPMEVCRDLALTEFSAVDSTVPRVVFWHDDRVPWRTPAGLSWNKAFYRDRKVVLLVRD
                     IRDAAVSRYFQVRHRGLLPYTAGIGDFLTEEEGSVRTCLTFWNIWHARQDIPASFLLTS
                     YERLTADTAGELARVLDFCGLPPTGQEALRTAVDFADFKAMRVMELSDALDTDRLRPAV
                     PGNPDSYKTRRGVIGGFRDYLTSGQIDYVNDATRRILAPPWQPAAFAADAAPHRPDRTL
                     "
ORIGIN
        1 cccgtccggc tgccgtctgc tcggtgacaa cttcacctcc acctgctcca cgcaactggc
       61 agccaaagcg cgccacgtcg acggcgttct gctcaagccc gaccagatcg gcctcatgag
      121 cggggtgtac cagttcgcct cgctggccag ggaccacggg ctgccgctga tcgcctcggc
      181 ccggtccgtg gaaaccgaca acccgctgat cagccacgtc gccacggagc tttgcctgga
      241 ctacctcaag gcgggcccgt accaggactt cagctccgtg atgcgatcga acgagctgct
      301 acgcgcaggt gcggcatgac cggcagcgtg gtgggatccg tcaccatcac cacggtgcgg
      361 cactcgctga cggctcacaa cacggccggc atcatcactg gccgcctcaa cgagcccctg
      421 tccgccgagg ggcgggctgc tgccagcggg tttgcccggg cccatggcac actgtcggcc
      481 gaggtcgtgc tctcctcccc tgcccggcga gccatcgaca ctgccaggct cgtcaccggg
      541 ctgccggaag aggacatcgt cctcgacgca cgctgccatg aacggcatta cggagccttg
      601 caaggcctgg accgagagca agtgtccgca tacgtagccc ggatcgtcta tgtggaagcg
      661 ggcgggacgc ggcattcagt caatccgccc ggcggggaga cgctcaccga gctccgggcc
      721 cgggcccacc gcttcctgat ggccgtcctg tccctgtcca cgacgtcggt cctggtgtcc
      781 tctcatggga ccttcctcca gcagttccac ggtctgctgc tcgagcgggg cacgatggag
      841 accctcgcct gccacatccg cagcctgcag atcgaccgct tcacgctccg cggcgagggc
      901 aggccacgac accagctgat ccacccgggc ctgagcaccg gcctggtctg gtgagcgggg
      961 ttccggtgcg tattcttgtt tgcacaggac gtgacctggt gacctgtgtg gcactgaacc
     1021 ggctgctacc tgcgctccaa gaccatacga ctgccgtcgc actgctgagg ctgcccccgg
     1081 tcgacttgag gtcgggcagc tctttggacc gcctgcggtg gcacctgcgg gaccttcctg
     1141 acaaggagtt cttccccgtg ctggaccggt tggactaccc accaggcgag ttgatgtcgt
     1201 ttcaccacct cgcaggcaac ctgggcgtac cgttccacga gatcgagaac atcaataccg
     1261 gtcacggcag ccgggtcgca cgggagttcc agccggacct gatcctgtcg gtgaagttcg
     1321 gccagatctt caaagagccg atcctttccc tcccttcttt cggcatcctc aacacccatt
     1381 ccggggccct tccgcagtat gcgggcctgt gggcacccgt ccgcaccctc cttgccggcg
     1441 agcgcaggtt taccagtacc ctgcacgtgg tcgaccaggg catcgacacc ggaccggtca
     1501 tcgcgagcgg acatctgcca gtcgagccaa accgttcact gttctggcat ctgcccaggc
     1561 tgtacgtgct ggggatcgag ctgttcctgc aggtgctgcc cggcctgacc aagtgcaccc
     1621 ggatcacagc acatcggcag gagaactccc ggcgccggta ctacggcaag ctgacaacgg
     1681 aagaggaagc cgccttcgag aaacagggct tccggtttct cgacgatcgc gactacgcgg
     1741 aaatcacccg acgcttcgga gtggatctct tcaccgtgag ctccgaatga cagggaagtg
     1801 cccaggcgta gcccagttcg gcgagggcag cccattgctc ggcattcagc cggtcacgcc
     1861 tggcatccaa ttggcggccg cccctgactg gcctccgtgc gccctgccgc aagggtggat
     1921 gtgaggtact gactgagtgg agtggcaaac ctcctggacg aggtgtgcgt catccggcgc
     1981 ctcccccgtc tcaacaggag ggttttctcg tgaaggctgt ccgccgcgtc accgtcaccc
     2041 aggccgcccc ggcactcgtc gcgctgcccg gcgtcggccc cgagaccgcc ggccagctgc
     2101 tggagaccgc gggcgacaat cccgaccgcc tcgtctccgg agccgccttc acccacccgt
     2161 gcgccaccgc tcccgtctcc gcgtcctccg gacgcacaga ccgccacagg ctcaaccacg
     2221 gcagcaaccg acacccgaac tccgctctgc acacgatcgt cctcgtccgc atgtccttcg
     2281 accactgcac ccgggaatac gtcgcccggc ggaccaccca ggggctgacg aagaaagaat
     2341 catgcgatgc ctgaaaccac ttgacgacct ataggagttt cacgccccaa ggccttcgag
     2401 gacgagatcg gcgcggcgcc gccaggtgta gcgctccagg aactgccgtc gggcctcgtc
     2461 accgagcgtc cggcgcagcg tctcgtcgcc gacgagctcg gtgagggcgt cggcccaggc
     2521 gtccggttcg tcgggcgggc gcaggaggca gttgacgcgg tcctggagga cttcgcgcag
     2581 gacgggaagg tccgaggcga tgatcgggcg gccgtgggac atgtactcga agagcttcat
     2641 gggggaggtc cagcgcccga tctcgtcgag cttgccccac gtgtacacct ttctctggtg
     2701 cggggcgagg gccacgtcga agagggggaa gtaggcgggg agggcggccg gcggccggtg
     2761 cccgtggaag tgcgcgttgg ggagcgggcg ggatccctcc cagtgggccc ggtcctcgga
     2821 ggtgccgccg accaggtgga actccaggcc ggggaggcgg tcggcgaggt cgaggatgag
     2881 gccgatgccg cggccggcgt agaggtgtcc gacgtagccg atccgcggga cgtccgggcg
     2941 gcccggcagc cgcggcgcgg cggctgcacc ggcgggcggt ggcgggtcgg cgcagtcggg
     3001 tgcgacgagg atcggcagcg cgcccaggtc gccgtgggtg ctccggaggt cacgcgccag
     3061 tgcgtgggtg atcacgacga ggcgggcgag ccggggcagg ccgaggagcg cgtgttcggt
     3121 tccgggtggc aggtccctgc ggatctggtg ggcctcgtac acgaaggggg cgaggtcgga
     3181 ggcgacagtg aggctgtagg gctcgcgtcc gtagacgagg tcggggacgg tgtcacggat
     3241 cagcaggtcg cgtatgcgct cggcgcgttt ccagtatccg gccggggtgt agtccggggc
     3301 ggggacggtg cggatgggga agcgatggcg gacgccgtag taggcgtacg ggtcgttggc
     3361 cggccaggag ccgggcaggg cgtacagggt gacgtcgtgc ccggcggcgg tgaacgcgtc
     3421 gcacatgcgc atgacgtgga cgccgttggc gaagaacgaa gggatgcttc cgccgtgcag
     3481 atacgcgatg cgcatgctct cctcgcgata ggccgtgagc ggtctgggag gggacggacg
     3541 ggggacactg ccgctcgcga tcccgccttc ccggatcgcc ggtcaaccct gaagaacgcg
     3601 tgttgacgtg gggcatcatc gcgcgtcagc gatcggcatc caagagcgac tgcgcaggag
     3661 aaggtggatt tcggctacgc gccattcacg cccaaagccc cccacacagg caggggttga
     3721 cacaacagca cacccctcgg cagcagcaaa caaccacgct ctccacataa agtgatgact
     3781 taattacacc ttgtgatcga atcgattccg tatctacctt ggcggcgccg ggtgtccccg
     3841 tcgacaggtg gagccggtca atcgaaggga gatcgagatg gggaacgaca tcgagcaggt
     3901 ccccgaggaa gaactgccgg agctggcgct gatgatcgac ataccctcgg actgacgccg
     3961 cttcactccc gggcgttggg ctggcgcgtt ccgacccaac gcccacctgt tcccgggatc
     4021 acggaggtat tcgcggtgcg agtggcgccc gacgcgcatc agagcgccga ggaatgctcg
     4081 gttctggcgc gcatgacccg atcggccctg acgagagccg aattgacggt cacccagaag
     4141 gaatcggcgc accccgccgt cgtcgagacc gcgcatgccg cccagcgtgt cctgcgtgcc
     4201 ggtcctgacc gggaacgcct cgacgggctc cgccgcatgc tcgatcgggc cagagccggg
     4261 atcgccgccg caccaccatc cgtactcccc tggcctgtcg acctcgccgc gccccacccc
     4321 catctcgaac gcagcgtcac ccgggccctg gagtccgtac cgaagcggga cggcgacttc
     4381 gagagcccgc atggctccgc ggtcgtcgca tggcgcgagc gcgacatctc cacgctccgc
     4441 gagacgatcg cgctcctggc ggcggcctgg cccgaggcac acgccgaact gcgggaaacc
     4501 ctcgtccaga tcgccctgtt ggacggaccc gcgatcgacg gtttcaccga cttcgtggtg
     4561 cacggtgccg tgttcatccg gcgcgaccgc ctggagacgg ggcgagacgc gctgccgggc
     4621 cccgtccgcc tggcggaggc actcgtccac gagggcgccc acacccggtg caacgcggcg
     4681 tccgtggccg gggagccctt cctgcggccc gcgcggggca gcgaaccgct cgtcaccacg
     4741 ccgttgcgga tcgaccccag gccgctgaac gggctcttcc agcaggtcgt ggtcctggcc
     4801 cggagccttc tgttgtaccg gcgcatgctc gacacggacg cgacggccgc ggcgtcggcg
     4861 gccgtgcggg cacgtcacga ccacgtggcc cgcgccgccg ctcagggcgt gcgcgccctg
     4921 tcggagcacc gcggcgcgct caccgaccgc ggggcgtccg tcctggagga cgccgccgga
     4981 ctgccccggg tgcgcgcctg atgtccacgc cgcggtggga gccacaggtc ttcgccccgt
     5041 acgccccctg gcccgaggtc gtcttcgcgc gcgtcgccgc ccgctcgccg cagttcgacg
     5101 gcgtctgcgc cgccggtgac cagcccgtcg tcgtcggcag cgcggccggg caccgtacgg
     5161 aacacgtcgt cgcgagcgcg cgcggcgaac tcctcgaacg cgtcggcaac atcctggcgg
     5221 gccgcgctgc cgaagcgaac gcgacggtcg tggcggccca cggggaactg cgccgtaaag
     5281 ggatacccgc actggatccc gccgagctcg cccccgcgga ctctccggac accgtccgca
     5341 gtgcccggcg gctgtgggtt ctcggccggt gggcccgtac cggcgacgag accttcgtac
     5401 ccgccggtgc cgtcttcctc caccaccggg cgccggtcgg ctgcgccccc ggccccggtg
     5461 ccgggtccac cggcgtcgcc tcgcaccccg atccacggtc ggccgcggac cacgccgcat
     5521 gggagacgct ggaacgtgac ctcgtccgca ggagctggta cggcatctcc ccggccccca
     5581 ccgcccttcc agcggcagcg atcggcctgc ccgcgccgct ggcgaagctg ctggacggaa
     5641 tcgggacgac ggcgaccgcc ctctgcctcc cctcccccgg cggatcccgg tgcgtcgtcg
     5701 tctgcctgca cgcacccgac cgcacgcggc aggcgttcgg cgcccgctgc ggaccggacg
     5761 acggggacct cgccccgctg gtcgaaaagg ccgcctacga ggccctgatg gtgcgttgga
     5821 gcgtccacac cccggtggcc cgcggcacct gggcgcgttg gcacggccgc gggtcgcccg
     5881 attcggctgt ccagcacgcg ctctgggcct atcaccggca ggacagcctg ggcctgtgga
     5941 ccgcacgggc accggcgcac ggccgccgtc ccgccgcgac ggcgggcggc gaaccggtcc
     6001 gctccggcat cgccgcgctg tccgcccaca ccggccggga cctcgtcctg gtggacacca
     6061 cctgcggacc ggcacgggac gcaggcgcga ccgtcgtacg ggtggtggct cccggggccc
     6121 gtgccctgcc caccggaccc gacggcgaac ggggcctgcc cgtcccgccg ggcccgcacc
     6181 ccttcggctg agaccacggc ccggtcacgg ccccggccac gcggcccggc cggccgcagg
     6241 acgaccgggc ccatcacagg acgaggagag acacgcgacc atgcccacgt cccaggacct
     6301 caccgccccg gcgacagcga cgaaccccga tgccgcgcat accgggaacg gacaccccgc
     6361 ccccgcggcg tgcggccccg gatacgccga cgcccacgcg gccgactacg accgctggtt
     6421 ctccaagccc ggcatcaccg gcgcgaccgt ggacaccctc gcgaatctcg cgggcgacgc
     6481 acccgtgctc gaactcggcg tcggcaccgg cagaatcgcc ctgcccctgg ccgagcgcgg
     6541 cctcgacgtc cacggcgtcg aggcgtcgca cgcaatgatc cggcggctgc gcgagaaacc
     6601 cggcgggacc cgaatcaaag tcacgatggg cgacttcgcc gacgtgccgc tcaccggcac
     6661 gttctcgctc gtctacgccg ccggtggcac gttcttcgaa ctcgcggacc gcgaggcgaa
     6721 acaacgctgc ctcgcggcgg tcgccgaccg tctcgcgccc ggcggagccc tcgtcctcga
     6781 ctcgcacgtg cccgaggccc ttgccgtcgc ggcggcgtcg ggcgtacccg aggtcgtctc
     6841 cgagaccgac gagcacctcg tcctctgcca tcgccggatc gacccatcca cccagaccta
     6901 ccggtcccac tacgtcatcc acgagaccga ccggacccgc cacctccgga tccacttcca
     6961 ctacgtgtca cccggcgaac tcgacctcat ggccgaacgg gccggcctcc gcctgataca
     7021 gcgtcaaggc aactgggcag gagggccgtt caccaaggac agcacttacc acgtctccgt
     7081 ctacggaccc cgctgacgtc gaccccgccc tgccgccaca cggccgccca cagcctccgg
     7141 agcagtcatg acggacaccg accccggccc caccaccggc ttccctctcc cgcaccgtga
     7201 cgggctcggt cccctgccgg agttcaccgc gctccgccgc gacgcaccct gcgcccggat
     7261 ccgcctgccg agcggagatc cggcctggct ggtcacccgg tacgcggacg tacgcaccgt
     7321 cttgggggat ccgcgcttca gccgcacccg cgccacccgc ggcacgggac cccgcatggc
     7381 ctccgtgccc accctgcccg actcgatcct cgccgccgac ccgcccgagc acacccggct
     7441 gcgcaagctc atcgccccgg ccttcaccgc ccgccgcgcc caggccatgc gacgggacat
     7501 ggcgcgactg gtctccggcc tcctggacgc gctcaccgcg cacgaccggc cggccgacct
     7561 cgtcccgctg ttcacccggc ccctccccct ggcggtcatc tgcgacctgc tcggcacgcc
     7621 ccggcaggac ggcgaccggc tcgacgcctg gtgcgacacc ctgcgcaacc tcaccgcggc
     7681 ccccgacgcc gcggtcaccg ccgcggtcga cgagatgacg ggctatctcg ccgcgctcgt
     7741 tgccgccaaa cgccgccacc ccgccgacga cgtcgtgagc accctgatcg ccgcacacga
     7801 cgacggcgaa cggctcaccc aggaagagct gatctccttc tgcctcgtgc tgctcgtcgg
     7861 cgggtacggc accacggcgg accgcctcgc cggcatggtc cacctcctgc tcgacgaacc
     7921 cggccgctat cgccgtctgt gccgcgcacc ggacacgatc ccccgggccg ccgaggaact
     7981 cctccgctac gcccagacca atgtgcaggc gaacctccgc gtcgccaccg aggacgtcga
     8041 actcggcggt gtcacgatcg ccgaaggcga agccgtgatg gccgtcaact cgtcggccaa
     8101 ccacgacgaa acggtcttcg aggaccccgg acggctcgac ctcgaccgtg cccacaaccc
     8161 ccacttgagc ttcggctacg gcgtccacca ctgcgtcggc gcgcaaatcg cccgtgtcca
     8221 gctccaggaa gccctggccg gactcgtcca ccgcctgccc gacctgcgcg ccgcggcacc
     8281 accgcgctgg aagaccggcc tcaagacccg ggccccccgc accctgcccg tcacctggtg
     8341 acccggccat gcccagcggc gacgaccccg ccacggcctc ggacgggacg ctccgcgccc
     8401 acatcgaccc ggagctctgc gtcggctccg gcatctgcct cgccaccgcc ccgcgccact
     8461 tcgaaccgac catggacgcg cgctcccgcg cccggcccga cgcaccgccc gccgacgacc
     8521 gggcacagga cgccgcggcc ctgtgcccga tggaggcgat ccacttcgtc cgggcccggg
     8581 agccgacacc acggaccgca ccgccggagg cacgctcctg atacggcagt ggagcatctc
     8641 cagcgggggg cgtagaagtc cgtaccgaac tcgcgtacac cggcgtcgga aacctccagc
     8701 agtacccagg caacctcgcc gaatccggcc gcccgcagac acttctcgta gacctcgcgg
     8761 cgcgggcggt tggcgacgaa cgagatcggc gggtcgagca gcgccgtgat gcggacttgc
     8821 gatccggtct cggcctcctc accggtgcag ctggcgctgc tcgcgcagtg tgcggggcgg
     8881 cctcgacgtt cttcatgccg gcgtcctcgg acatccttac ggacatcgtt ccggcggaga
     8941 cgcggtcggc ggcgaacgca ctgctgcggt tcgggcagac cgcggtgaag gtgggcggcc
     9001 cggcggccgg cgcggcgttg gtggcggtcg tcggcccgcc atggggcatc gcctgggacg
     9061 ccttgtcgtt cgccgggtcg gccgtgctgc tctcacggct cacggtccgc ggtgccactg
     9121 tgacacgtca gccggagttc cggcaccaac tgcgcgacgg gtgggcgggc ttccgatcac
     9181 ggcgtcggct ggcgatcatg acccggcagg ccgctctggc cgtccctgtg aggatggccg
     9241 gccaccagct cctggggccg ctgtatgggc agcgtgtgct gggcggcgcc agtgcctggg
     9301 gcacggtaat gtccgccttc gcggcgggcc tggtcagcgg tgccgacgcg gcgctgtggt
     9361 ggcggccgcg cggcgtcggg atcgtggggt gcgcgggcac gggggcgatg gcggctccgc
     9421 ctgcgacgat ggcactgcac acgggggcga ccggtctcgt ggtgacgacc gcatcgacag
     9481 gggccgcggt gtcgctgtcg atgacggggt ggttgcagga ggtgatcccc ctggaccgcg
     9541 tgacagcgga tacggctttc ggccagtcgg tgacgctgcc gctcgactat ctcgcggcgt
     9601 cccccgccgc ccatgtgctg ggcctgggcg cggcactgat cgccgtattg gtggtgctgc
     9661 tgccggaggt acggctgctg ggcaggcccg tcatagtgtc cggtcggggc ggtgcggggc
     9721 ggcgtccgcc gcgaaggccg cgggctgcca cggcggtgcg aggatccgac gcgtggcgtc
     9781 gttcacgtag tcgatctgcc cggacgtgag gtagtcgcgg aacccgccga tgacgccgcg
     9841 gcgggtcttg taggagtcgg ggtttccggg aacggcgggg cgcagcctgt ccgtgtccag
     9901 cgcgtcggag agttccatga cacgcatggc tttgaagtct gcgaagtcga ccgcggtgcg
     9961 cagcgcctcc tggccggtcg gaggcagtcc gcagaagtcg agcacgcgcg cgagttcgcc
    10021 ggcagtgtcg gcggtcagcc gctcgtacga ggtgagcagg aacgaggcgg gtatgtcctg
    10081 ccgggcgtgc cagatgttcc agaaggtcag gcaggtcctg acgctgcctt cttcctcggt
    10141 caggaagtcc ccgattcccg cggtgtacgg gaggagaccg cggtggcgca cctggaaata
    10201 gcgggatacg gcggcgtccc ggatgtcgcg gaccaggagg accactttgc ggtcgcggta
    10261 gaaagccttg ttccaggaca gtccggccgg ggttcgccag gggacccggt cgtcgtgcca
    10321 gaagacgacg cgcgggacgg tggagtccac cgcgctgaat tcggtcagcg ccaggtcccg
    10381 gcagacctcc atggggattc cgcgcgccgt ggagatggcc ttggccatga gcacccgcag
    10441 ccaggtgcgg ccgcacttgg ggaacgagat gacgtagacg tcgccttccg cgtcggcggg
    10501 tttcgcgggc ccgccggagg gcgtggggag taccacggtg gtcctttcct gtcggtcatg
    10561 tccagggggt gttctcggcg gtggctccgc ggttgtggcg tgggaagggc tcgccgggga
    10621 cgggcaggcc gaggaaggga gcgagccgct cgtatccctg gccggcggcg atatcgagga
    10681 ccagtaggtt gtcccggccg gcgaagtggc gtgcgacttc ggcggcgtgc tgccgggcga
    10741 tccggcggaa ccgttccggg tggaaggcgt agtagccgta gaccgcgccc cgcaggaagc
    10801 gctggaggtc caggcaggtc tgtccgtggt ccgctttgct gtcgactttg ctgtcgacgg
    10861 gccgctccca gtgcttccgg caggagcgca gccggctctc ttcctcgctg accgtgagga
    10921 cgaacttcgc gcccggccac aggagatcca gttcctggta gccgggtacg gcggtgatgt
    10981 cggtgatccc gtcgtaccgg tcgagcagcg ggaagcgggc ggtgccggcc agcagcgtcg
    11041 tgtaggtggt ggggtcggag gggtagcggg cgacgtcgaa gcccagggtg tgcagggcgc
    11101 tggtgagaga ggtggtgccg gtgcacgaga ggccgatgcc gaagactttc ggcttcgtgc
    11161 ggcgcgagag ggacgcggcg gtgcgtgcgg g
//
