GCF_003335105.1 - 5 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

NZ_QFWQ01000003.1 (Rhodanobacter denitrificans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 10.1	RiPP-like	56,613	67,479
Region 10.2	terpene-precursor	667,230	688,129

NZ_QFWQ01000005.1 (Rhodanobacter denitrificans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 12.1	acyl_amino_acids	429,991	490,836

NZ_QFWQ01000007.1 (Rhodanobacter denitrificans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 14.1	arylpolyene	202,587	243,780	Low	xanthomonadin I	other:other

NZ_QFWQ01000009.1 (Rhodanobacter denitrificans)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 16.1	lassopeptide	35,343	57,777

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 10.1	RiPP-like	56,613	67,479
Region 10.2	terpene-precursor	667,230	688,129
Region 12.1	acyl_amino_acids	429,991	490,836
Region 14.1	arylpolyene	202,587	243,780	Low	xanthomonadin I	other:other
Region 16.1	lassopeptide	35,343	57,777

No secondary metabolite regions were found in these records:: NZ_QFWQ01000001.1 (Rhodanobacter denitrificans); NZ_QFWQ01000010.1 (Rhodanobacter denitrificans); NZ_QFWQ01000011.1 (Rhodanobacter denitrificans); NZ_QFWQ01000012.1 (Rhodanobacter denitrificans); NZ_QFWQ01000013.1 (Rhodanobacter denitrificans); NZ_QFWQ01000014.1 (Rhodanobacter denitrificans); NZ_QFWQ01000015.1 (Rhodanobacter denitrificans); NZ_QFWQ01000002.1 (Rhodanobacter denitrificans); NZ_QFWQ01000020.1 (Rhodanobacter denitrificans); NZ_QFWQ01000004.1 (Rhodanobacter denitrificans); NZ_QFWQ01000006.1 (Rhodanobacter denitrificans); NZ_QFWQ01000008.1 (Rhodanobacter denitrificans)

Compact view

NZ_QFWQ01000003.1 - Region 1 - RiPP-like

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Location: 56,613 - 67,479 nt. (total: 10,867 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

DEO45_RS02525: pyridine nucleotide-disulfide oxidoreductase

smcogs: SMCOG1175: pyridine nucleotide-disulfide oxidoreductase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002804.2	0.28	other (other)	protegencin	Pseudomonas protegens CHA0
BGC0002470.2	0.26	other (other)	synechobactin C9, synechobactin C11, synechobactin 13, synechobactin 14, synechobactin 16, synechobactin A, synechobactin B, synechobactin C	Synechococcus sp. PCC 7002
BGC0002788.2	0.22	PKS	14-hydroxyisochainin	Streptomyces peucetius subsp. caesius ATCC 27952
BGC0002559.2	0.19	PKS	lenoremycin	Streptomyces hygroscopicus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002804.2	0.28	other (other)	protegencin	Pseudomonas protegens CHA0
BGC0002470.2	0.26	other (other)	synechobactin C9, synechobactin C11, synechobactin 13, synechobactin 14, synechobactin 16, synechobactin A, synechobactin B, synechobactin C	Synechococcus sp. PCC 7002
BGC0002788.2	0.22	PKS	14-hydroxyisochainin	Streptomyces peucetius subsp. caesius ATCC 27952
BGC0002559.2	0.19	PKS	lenoremycin	Streptomyces hygroscopicus

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 19.02 out of 25.31

Confidence: medium

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 24.73 out of 30.22

Confidence: medium

Additional weak hits (1):

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.79 out of 29.65

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CatR	H2O2-responsive repressor
ClgR	Caseinolytic protease gene regulator

NZ_QFWQ01000003.1 - Region 2 - terpene-precursor

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Location: 667,230 - 688,129 nt. (total: 20,900 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

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Gene overview

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MIBiG comparison

KnownClusterBlast

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Terpene

TFBS Finder

Gene/CDS overview

Filter:

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: transferase

DEO45_RS05430: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

Ligases: synthetase

DEO45_RS05455: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.39	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000640.3	0.37	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0000630.3	0.36	terpene	(2R,3S,3'S)-2-hydroxyastaxanthin	Paracoccus haeundaensis
BGC0000639.3	0.34	terpene	carotenoid	Enterobacteriaceae bacterium DC260
BGC0000641.3	0.34	terpene	carotenoid	Enterobacteriaceae bacterium DC416
BGC0000642.3	0.34	terpene	carotenoid	Enterobacteriaceae bacterium DC413
BGC0000638.3	0.34	terpene	carotenoid	Pantoea ananatis
BGC0000635.3	0.33	terpene	carotenoid	Paracoccus sp. N81106

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.39	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000640.3	0.37	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0000630.3	0.36	terpene	(2R,3S,3'S)-2-hydroxyastaxanthin	Paracoccus haeundaensis
BGC0000639.3	0.34	terpene	carotenoid	Enterobacteriaceae bacterium DC260
BGC0000641.3	0.34	terpene	carotenoid	Enterobacteriaceae bacterium DC416
BGC0000642.3	0.34	terpene	carotenoid	Enterobacteriaceae bacterium DC413
BGC0000638.3	0.34	terpene	carotenoid	Pantoea ananatis
BGC0000635.3	0.33	terpene	carotenoid	Paracoccus sp. N81106

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor		FPP	C15	acyclic	PP

Genes with terpene domains:

DEO45_RS05455: PT_FPPS_like

Domain 1 (35 - 297): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: Farnesyl diphosphate synthase, bacterial

Reactions:: DMAPP, IPP → FPP

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 16.73 out of 18.46

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.92 out of 20.47

Confidence: medium

Additional weak hits (3):

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 18.70 out of 36.54

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.03 out of 21.55

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.77 out of 24.78

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
RpoN	Regulator of nitrogen assimilation and virulence
VqsM	Regulator of quorum-sensing signalling systems

NZ_QFWQ01000005.1 - Region 1 - acyl_amino_acids

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Location: 429,991 - 490,836 nt. (total: 60,846 nt) Show pHMM detection rules used

acyl_amino_acids: NasY

Legend:

core biosynthetic genes

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other genes

resistance

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Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

DEO45_RS09445: pyridine nucleotide-disulfide oxidoreductase

smcogs: SMCOG1175: pyridine nucleotide-disulfide oxidoreductase

DEO45_RS09450: pyridine nucleotide-disulfide oxidoreductase

smcogs: SMCOG1175: pyridine nucleotide-disulfide oxidoreductase

Transferases: acyltransferase

DEO45_RS09300: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001591.4	0.44	other (other)	fatty acid enol ester, N-tetradecanoyl tyrosine	uncultured bacterium CSLC2
BGC0002334.3	0.44	other (other), ribosomal	heptyltyrazolone, nonyltyrazolone, undecyltyrazolone, (Z)-ω-6-undecenyltyrazolone	Pseudoalteromonas rubra
BGC0001687.3	0.44	other (other)	N-tetradecanoyl tyrosine	uncultured bacterium CSL12
BGC0002423.3	0.42	other (other)	arsinothricin	Burkholderia gladioli
BGC0001588.3	0.41	saccharide	exopolysaccharides	Burkholderia pseudomallei 1026b
BGC0001589.3	0.39	saccharide	exopolysaccharides	Burkholderia cenocepacia J2315
BGC0001377.5	0.38	PKS	uncialamycin	Streptomyces uncialis
BGC0002000.4	0.37	PKS	setomimycin	Streptomyces aurantiacus JA 4570
BGC0000559.4	0.37	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0000845.5	0.37	other (non-nrp beta-lactam)	clavulanic acid	Streptomyces clavuligerus ATCC 27064

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001591.4	0.44	other (other)	fatty acid enol ester, N-tetradecanoyl tyrosine	uncultured bacterium CSLC2
BGC0002334.3	0.44	other (other), ribosomal	heptyltyrazolone, nonyltyrazolone, undecyltyrazolone, (Z)-ω-6-undecenyltyrazolone	Pseudoalteromonas rubra
BGC0001687.3	0.44	other (other)	N-tetradecanoyl tyrosine	uncultured bacterium CSL12
BGC0002423.3	0.42	other (other)	arsinothricin	Burkholderia gladioli
BGC0001588.3	0.41	saccharide	exopolysaccharides	Burkholderia pseudomallei 1026b
BGC0001589.3	0.39	saccharide	exopolysaccharides	Burkholderia cenocepacia J2315
BGC0001377.5	0.38	PKS	uncialamycin	Streptomyces uncialis
BGC0002000.4	0.37	PKS	setomimycin	Streptomyces aurantiacus JA 4570
BGC0000559.4	0.37	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0000845.5	0.37	other (non-nrp beta-lactam)	clavulanic acid	Streptomyces clavuligerus ATCC 27064

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 18.15 out of 18.46

Confidence: strong

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.38 out of 19.26

Confidence: medium

Additional weak hits (12):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.70 out of 26.33

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.91 out of 27.78

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.78 out of 21.55

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.21 out of 20.01

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 20.44 out of 29.25

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.30 out of 21.84

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.60 out of 30.22

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 21.06 out of 27.55

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.84 out of 27.78

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.54 out of 28.19

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
RpoN	Regulator of nitrogen assimilation and virulence

NZ_QFWQ01000007.1 - Region 1 - arylpolyene

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Location: 202,587 - 243,780 nt. (total: 41,194 nt) Show pHMM detection rules used

arylpolyene: (APE_KS1 or APE_KS2)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

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NRPS/PKS domains

Gene/CDS overview

Filter:

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

DEO45_RS12845: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: transferase

DEO45_RS12890: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

Hydrolases: hydrolase

DEO45_RS12795: amidohydrolase

smcogs: SMCOG1244: amidohydrolase

Isomerases: mutase

DEO45_RS12805: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Ligases: synthase

DEO45_RS12830: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

DEO45_RS12840: polyprenol-monophosphomannose synthase ppm1

smcogs: SMCOG1123: polyprenol-monophosphomannose synthase ppm1

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001164.3	0.58	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.58	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0000840.5	0.52	other (other)	xanthomonadin I	Xanthomonas oryzae pv. oryzae
BGC0001217.5	0.50	other (other)	PreQ0 Base	Streptomyces qinglanensis
BGC0001400.3	0.45	PKS	citreoviridin	Aspergillus terreus NIH2624
BGC0001987.2	0.44	PKS	koreenceine A, koreenceine B, koreenceine C, koreenceine D	Pseudomonas koreensis
BGC0002008.3	0.42	other (other)	aryl polyenes	Xenorhabdus doucetiae
BGC0000836.5	0.42	other (other)	APE Ec	Escherichia coli CFT073
BGC0002711.2	0.42	PKS	nostovalerolactone, 9-dehydronostovalerolactone	Nostoc punctiforme PCC 73102
BGC0000243.4	0.41	PKS	nonactin, monactin, dinactin, trinactin, tetranactin	Streptomyces griseus subsp. griseus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001164.3	0.58	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.58	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0000840.5	0.52	other (other)	xanthomonadin I	Xanthomonas oryzae pv. oryzae
BGC0001217.5	0.50	other (other)	PreQ0 Base	Streptomyces qinglanensis
BGC0001400.3	0.45	PKS	citreoviridin	Aspergillus terreus NIH2624
BGC0001987.2	0.44	PKS	koreenceine A, koreenceine B, koreenceine C, koreenceine D	Pseudomonas koreensis
BGC0002008.3	0.42	other (other)	aryl polyenes	Xenorhabdus doucetiae
BGC0000836.5	0.42	other (other)	APE Ec	Escherichia coli CFT073
BGC0002711.2	0.42	PKS	nostovalerolactone, 9-dehydronostovalerolactone	Nostoc punctiforme PCC 73102
BGC0000243.4	0.41	PKS	nonactin, monactin, dinactin, trinactin, tetranactin	Streptomyces griseus subsp. griseus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 21.57 out of 23.79

Confidence: strong

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 26.08 out of 30.22

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 20.28 out of 23.43

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.28 out of 23.05

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.48 out of 23.79

Confidence: medium

Additional weak hits (11):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.09 out of 21.55

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.40 out of 30.11

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.20 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.52 out of 29.38

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 18.61 out of 30.97

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 21.54 out of 36.53

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.10 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.17 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.08 out of 26.06

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.66 out of 25.88

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 21.46 out of 33.14

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

TFBS Finder

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
AfsR	Pleiotropic regulatory for antibiotic production
BldD	Development and antibiotic global regulator
ClgR	Caseinolytic protease gene regulator
IolR	Regulator of the inositol catabolism

NZ_QFWQ01000009.1 - Region 1 - lassopeptide

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Location: 35,343 - 57,777 nt. (total: 22,435 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lasso peptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase

DEO45_RS14195: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000577.3	0.65	ribosomal	sphingonodin II	Sphingobium japonicum UT26S
BGC0000580.3	0.65	ribosomal	xanthomonin II, xanthomonin I	Xanthomonas gardneri ATCC 19865
BGC0000573.3	0.61	ribosomal	caulonodin I, caulonodin II	Caulobacter sp. K31
BGC0000574.3	0.58	ribosomal	caulosegnin I, caulosegnin II, caulosegnin III	Caulobacter segnis ATCC 21756
BGC0001786.3	0.56	ribosomal	subterisin	Novosphingobium subterraneum
BGC0000570.3	0.51	ribosomal	astexin-1	Asticcacaulis excentricus CB 48
BGC0000576.3	0.51	ribosomal	rubrivinodin	Rubrivivax gelatinosus IL144
BGC0002350.2	0.19	NRPS, PKS, saccharide	bleomycin A2, bleomycin B2	Streptomyces mobaraensis NBRC 13819 = DSM 40847

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000577.3	0.65	ribosomal	sphingonodin II	Sphingobium japonicum UT26S
BGC0000580.3	0.65	ribosomal	xanthomonin II, xanthomonin I	Xanthomonas gardneri ATCC 19865
BGC0000573.3	0.61	ribosomal	caulonodin I, caulonodin II	Caulobacter sp. K31
BGC0000574.3	0.58	ribosomal	caulosegnin I, caulosegnin II, caulosegnin III	Caulobacter segnis ATCC 21756
BGC0001786.3	0.56	ribosomal	subterisin	Novosphingobium subterraneum
BGC0000570.3	0.51	ribosomal	astexin-1	Asticcacaulis excentricus CB 48
BGC0000576.3	0.51	ribosomal	rubrivinodin	Rubrivivax gelatinosus IL144
BGC0002350.2	0.19	NRPS, PKS, saccharide	bleomycin A2, bleomycin B2	Streptomyces mobaraensis NBRC 13819 = DSM 40847

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lasso peptide predictions

Lassopeptide(s) for DEO45_RS14205

DEO45_RS14205

Leader:

MDTNENIRSNAQDDVIELGVASVETK

Full core:

GPGPSNEGTGQGIPFVPG

Putative macrolactam:

GPGPSNE

DEO45_RS14205

Cleaved off residues:

ISEE

antiSMASH-DB 4.0 matches for DEO45_RS14205

100.0%

NZ_QFWQ01000009.1:

Lassopeptides: DEO45_RS14205_lassopeptide

GPGPSNEGTGQGIPFVPG

57.9%

NZ_LMGH01000013.1:

Lassopeptides: ASD55_RS18465_lassopeptide

GPGPSNEGTGQGIPFVPG

GPG + EG G G P VPG

GPG-ATEGNGHGFPIVPG

44.4%

NC_020541.1:

Lassopeptides: R2APBS1_RS19990_lassopeptide

NEGTGQGIPFVPG

EG GQG P +PG

TEGGGQGKPMIPG

with 5 more sequence matches

44.4%

NZ_CP113061.1:

Lassopeptides: OVA03_RS07770_lassopeptide

GPSNEGTGQGIPFVPG

G N G G PF+PG

GSENAGEEHGEPFLPG

44.4%

NZ_JAETWA010000022.1:

Lassopeptides: JMO03_RS05165_lassopeptide

NEGTGQGIPFVPG

EGTG+G P +PG

QEGTGEGRPVIPG

38.9%

NZ_CP019797.1and 2 others:

NZ_JAACYG010000056.1 Lassopeptides: GXB84_RS17155_lassopeptideNZ_LMLT01000004.1 Lassopeptides: ASF01_RS18080_lassopeptide

Lassopeptides: B1L07_RS13675_lassopeptide

EGTGQGIPFVPG

E TG G+P +PG

EPTGPGLPMIPG

38.9%

NZ_SMBO01000018.1and 1 other:

NZ_SODQ01000013.1 Lassopeptides: EDF57_RS23870_lassopeptide

Lassopeptides: EDF58_RS24335_lassopeptide

GPGPSNEGTGQ

GP PS+E GQ

GPQPSDEALGQ

11.1%

NZ_CP010407.1and 2 others:

NZ_CP010407.1 Lanthipeptides: allorf_8470819_8470980_lanthipeptideNZ_CP010407.1 Lanthipeptides: allorf_8470819_8470980_lanthipeptide

Lanthipeptides: allorf_8470819_8470980_lanthipeptide

NEGTGQGIPFVPG

+ G QG+ + PG

SRGASQGLTYCPG

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.39 out of 19.26

Confidence: strong

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 21.67 out of 28.19

Confidence: medium

Additional weak hits (12):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.59 out of 27.78

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.31 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 19.08 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.64 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 19.50 out of 29.38

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 18.37 out of 26.06

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 20.06 out of 30.25

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.54 out of 26.06

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.44 out of 30.22

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 18.39 out of 26.06

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.14 out of 28.19

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 22.41 out of 36.53

Confidence: weak

Lasso peptides

RREFinder

TFBS Finder

Core peptide predictions

DEO45_RS14205 - Class II: Cleavage pHMM score: 12.70; RODEO score: 23; Monoisotopic mass: 1648.8 Da; Molecular weight: 1649.8 Da; Number of bridges: 0

RRE predictions

: RRE type: Lasso_Fused_RRE; Location: 0 - 66; Score: 54.7

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
CRP	Cyclic AMP receptor protein
ZuR variant 1	Zinc-responsive repressor