LOCUS       NZ_CP030862            10834 bp    DNA     linear   CON 04-MAY-2024
DEFINITION  Streptomyces globosus strain LZH-48 chromosome, complete genome.
ACCESSION   NZ_CP030862
VERSION     NZ_CP030862.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces globosus
  ORGANISM  Streptomyces globosus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 6863360)
  AUTHORS   Ran,K., Li,Z., Wei,S. and Dong,R.
  TITLE     Draft genome Sequence of streptomyces globosus LZH-48
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6863360)
  AUTHORS   Ran,K., Li,Z., Wei,S. and Dong,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2018) Institute of Biophysics, Dezhou University,
            566 DaXuexi RD, Dezhou, Shandong 253023, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT Portal v. version 2.2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 110.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003325375.1-RS_2024_05_04
            Annotation Date                   :: 05/04/2024 02:22:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,671
            CDSs (total)                      :: 6,576
            Genes (coding)                    :: 6,458
            CDSs (with protein)               :: 6,458
            Genes (RNA)                       :: 95
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 41 of 118
            Pseudo Genes (incomplete)         :: 101 of 118
            Pseudo Genes (internal stop)      :: 11 of 118
            Pseudo Genes (multiple problems)  :: 32 of 118
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:39:31
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1633114
            Orig. end                         :: 1643948
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP030862.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10834
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="5"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10834
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10834
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5834](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5834)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    824..839
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.27"
                     /tool="antismash"
     gene            complement(1026..1880)
                     /locus_tag="C0216_RS07620"
                     /old_locus_tag="C0216_07615"
     CDS             complement(1026..1880)
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018384427.1"
                     /locus_tag="C0216_RS07620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C0216_07615"
                     /product="SAM-dependent methyltransferase"
                     /protein_id="WP_114054525.1"
                     /transl_table=11
                     /translation="MPVLNPVERTALLTAALRAAETRREDRLYEDPYAAGLVGEAGPEL
                     LREIREATFPPGRPRTLPSTPDYNAIRTRFFDDFLQEAARAPETTQIVLAPAGMDSRAY
                     RLPWPGRVRYFEVDRQAVLDFKAERLGGAEPRVDHRTVAVDLTSDGWEDRLAAAGYDPS
                     VPSTWLLEGLLYYIPEADAHRMLERVAAVSAPGSRIAADIVNAAALTLPHMRGLLDVFA
                     GWGCPWVFGTDEPEDLFARYGFDVRAVQPGEPGADFGRWPDPVPPRAVRRVRRVFFVHG
                     RRR"
     gene            complement(1934..3616)
                     /locus_tag="C0216_RS07625"
                     /old_locus_tag="C0216_07620"
     CDS             complement(1934..3616)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018384428.1"
                     /locus_tag="C0216_RS07625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C0216_07620"
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="WP_114054526.1"
                     /transl_table=11
                     /translation="MNVSSVWHAPGHAVRYRERVFAARTRLTRDHTDLPEAQRRALTDL
                     VGFNASTAFGRAHGLGRVLTMEDFRSAVPVRDYADHAPLIERTAAGERNLLSADTPAVY
                     FTSSGSTGAHKKVPVTPRFMKTTFMPFYYAAWAPLVEHFPEVLERPDAVLNLKHDPLTA
                     PPTTADGRPHVGASQVDFGARFGEPLSAEPGTGAPWAALPVPTDPADHLERAYLRLRLA
                     VQADLRCMIGINPAMVAAVPYQLNLWWPRIVKEVRDGTLGGLPHGRPDPARAAELERLA
                     DSSGRVRPAQVWPRMRALFCWTTGVASLYLPALSGEFGADVAVLPAPVAASEGPVGVAL
                     DRHPSAGSLTASASVYEFVPAEEDLRPDSDTLLPDELEAGRDYHVVFSHVGGLYRYAVG
                     DVVRVVDTAGGVPRVEYAGRGGRSDAAGERLRDAEAVRALHTALRPGGLALRNAACRVE
                     PSPGGTPHYVFAVSPRGPWPPAGAARFTAALDGALSAESAGYAAARAAGRLAAPSLRLL
                     DAEAFDRDWHAAVASGIRPTQVKDRLFRQDDALWGRLTAAPNG"
     gene            complement(3613..4242)
                     /locus_tag="C0216_RS07630"
                     /old_locus_tag="C0216_07625"
     CDS             complement(3613..4242)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018384429.1"
                     /locus_tag="C0216_RS07630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C0216_07625"
                     /product="hypothetical protein"
                     /protein_id="WP_114054527.1"
                     /transl_table=11
                     /translation="MSGPRFHVLLGPDGAGKSSVMRRLAAQLPEWRLVSADRAFVAPEH
                     DLVNRLRQDVHEHLLPGLGTAYSADFMASVLQTAVVHLRDQLDGPDPRVPVLVDSYYYK
                     ILAKCRMAGVSDSRLYSWWRSFPQPARVVFLDVSPETAWHRRADGARLNALEYEGRDGR
                     WADFERYQKNLRKILLEEVRDVPVTVLAEQSDPDRAARAVLEVLAA"
     gene            complement(4239..4922)
                     /locus_tag="C0216_RS07635"
                     /old_locus_tag="C0216_07630"
     CDS             complement(4239..4922)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018556338.1"
                     /locus_tag="C0216_RS07635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C0216_07630"
                     /product="HAD-IB family hydrolase"
                     /protein_id="WP_114054528.1"
                     /transl_table=11
                     /translation="MSDTTRPHTVFSDVDETLITVKSMFDFLHYRLVRLHGAAGEEEYE
                     RIMAVLRRRAADGVPREEINRAYYRHYAGEDVAAVTALADDWYTERAASTRGFWIDSTR
                     EALHRHRAAGASVVLVSGSFPPLLEPLAREIGADAILCTRPLADGGTYTGEVAVPVIGE
                     GKREAVLAHLASRPDTDPGDCWAYGDHVSDLPMLELVGHPVAVGDDPELRERLALRAET
                     TAAAR"
     gene            complement(5001..5834)
                     /locus_tag="C0216_RS07640"
                     /old_locus_tag="C0216_07635"
     CDS             complement(5001..5834)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018556339.1"
                     /locus_tag="C0216_RS07640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C0216_07635"
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="WP_114054529.1"
                     /sec_met_domain="AfsA (E-value: 1.2e-15, bitscore: 55.7,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQETSQAGEARTGALFLVGDVFAEFAHHEGVFTVSQLAKRIRSG
                     AFPDDAGSGPVRIALGQGVSLFDVAFIRDTLDRRGLGDRVAIDDQALHTRAGREIAHKH
                     RPENVLISDPRPAGTDWYEADLLVDAGNEVMSDHVTGQHMQGMLATEAARQMFIAVAEQ
                     FYLPTEAVGSRYFVIDSFSTRYRNFLFPLPAVVRCHVLSHRSPHHTRTAFHCALSVTQG
                     GSETAGMDVRFTAFDTQVSHAKETRAAQRSLQDGVALATAEAAATAPARSEVGAS"
     gene            complement(5924..6142)
                     /locus_tag="C0216_RS07645"
                     /old_locus_tag="C0216_07640"
     CDS             complement(5924..6142)
                     /GO_function="GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018556340.1"
                     /locus_tag="C0216_RS07645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C0216_07640"
                     /product="tautomerase family protein"
                     /protein_id="WP_114054530.1"
                     /transl_table=11
                     /translation="MPIISVTTWDGQDDAQSRELLEELTRTVRRVTGAPLDKITVYIQE
                     VPRNRWAEGGALGSDPKFPELSRRTAE"
     gene            complement(6191..7093)
                     /locus_tag="C0216_RS07650"
                     /old_locus_tag="C0216_07645"
     CDS             complement(6191..7093)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436517.1"
                     /locus_tag="C0216_RS07650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C0216_07645"
                     /product="hypothetical protein"
                     /protein_id="WP_114054531.1"
                     /transl_table=11
                     /translation="MSETATAAAPAGAATPHRREVHELSPTTQGPLYPPSEVMDGDGNF
                     LVVGMVNRPAPGGGTAPGWGAAVVSPASPVPEFGRLAPYTVVRELDTDPDGADRDVVLH
                     TLPLPLPCNNYPMVFAPEQLPDADRVVRPSHAFHEVPIPDLRAEDGPKVTEPVTFGEWM
                     RASGTLEVAVTPDGHAGTFDFRFSRLVPNSVYTVMSLRARDLDPAGPTRPGPLGVPNVF
                     TTDAEGSGSYHATLPNPFPDPDLPGANRIINVVVLWMSYQRSYGGAIGEFGLGGDIHAH
                     LKLRGPSFPDLRTTAAPQT"
     gene            complement(7086..7706)
                     /locus_tag="C0216_RS07655"
                     /old_locus_tag="C0216_07650"
     CDS             complement(7086..7706)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018556342.1"
                     /locus_tag="C0216_RS07655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C0216_07650"
                     /product="hypothetical protein"
                     /protein_id="WP_114054532.1"
                     /transl_table=11
                     /translation="MSSTLLVLNEVAAAPGKADHVLEQWQQFNRKEPVAGRALYRSLDD
                     DNLLEITPVAEVSEVAALNARWHELWDLVSEDLATDFRRQLLDFVEAPKDVAEPVPATP
                     YVQLRHIEVKPREYAAYREWRENTIFNVVRRADEVKAFLAYHSLLSTEPGVMFVSGFEC
                     APEQYQAVFTSPEYQEIVQQAGDRYIVGGESGLYTRVYQRADV"
     gene            complement(7902..8144)
                     /locus_tag="C0216_RS07660"
                     /old_locus_tag="C0216_07655"
     CDS             complement(7902..8144)
                     /NRPS_PKS="Domain: PP-binding (12-71). E-value: 6.3e-11.
                     Score: 34.4. Matches aSDomain:
                     nrpspksdomains_C0216_RS07660_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018556343.1"
                     /locus_tag="C0216_RS07660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C0216_07655"
                     /product="phosphopantetheine-binding protein"
                     /protein_id="WP_114054533.1"
                     /sec_met_domain="PP-binding (E-value: 6e-08, bitscore:
                     31.5, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MENSIVSRVVTVLGELEVSTAGVTPDTTFEAMEIDSLLLEELALR
                     LQLSFGVEIEMGELVPEQTVAEAAAVIASKGVPVA"
     aSDomain        complement(7932..8108)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C0216_RS07660_PP-binding.1"
                     /evalue="6.30E-11"
                     /label="C0216_RS07660_PP-binding.1"
                     /locus_tag="C0216_RS07660"
                     /protein_end="71"
                     /protein_start="12"
                     /score="34.4"
                     /tool="antismash"
                     /translation="LGELEVSTAGVTPDTTFEAMEIDSLLLEELALRLQLSFGVEIEMG
                     ELVPEQTVAEAAAV"
     gene            complement(8380..9240)
                     /locus_tag="C0216_RS07665"
                     /old_locus_tag="C0216_07660"
     CDS             complement(8380..9240)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018556344.1"
                     /locus_tag="C0216_RS07665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C0216_07660"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_114054534.1"
                     /transl_table=11
                     /translation="MQTAASAPASGLLRRARLRHFLRARREQITPEEVGLVNFDRRRTP
                     GLRREEVAALAGVGVSWYTWLEQGRDINVSEGIVNAVSNALRLEDCERDYFHRLAGHNP
                     PPRTPDRGQEGGHARMRALVEGWSPNPAYITDRYGRVELASRSARSLFHIGTRGHNCVV
                     KFFTESATREKYPDTEEVGRGLVAQFRAQSARFPQDPEFGRIAGRLSEMSPHFAELWDC
                     HEVVGPNQGLRRIALPQAGVTVFDRVVMRVPESPESLLTLYIAQSGAERAGRRDEPAGF
                     GAEAA"
     gene            9419..10213
                     /locus_tag="C0216_RS07670"
                     /old_locus_tag="C0216_07665"
     CDS             9419..10213
                     /NRPS_PKS="Domain: Thioesterase (29-246). E-value: 1.3e-35.
                     Score: 115.8. Matches aSDomain:
                     nrpspksdomains_C0216_RS07670_Thioesterase.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Thioesterase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1004: thioesterase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018556345.1"
                     /locus_tag="C0216_RS07670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C0216_07665"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_114054535.1"
                     /sec_met_domain="Thioesterase (E-value: 2.5e-33, bitscore:
                     114.6, seeds: 16, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 2.7e-07, bitscore:
                     29.9, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFHTAENIPAATAAGTAERVPRRSRTARLRLFCFHHAGGSPWSFA
                     GWGQHLGRGVEVVPVSMPPRSSAPGGVPGAVSTMDALVREVTRQLTPFLDGPYAFYGHS
                     MGSLVAHGAAQRIVAAGRRPPASFVAGAHRAPHLQAAPGPTVGLSDSASLELLLGLGGA
                     GRLLRESPARMRSVVHRLREDLRVAATHRNPVAGHRPLPCPVHAMLGDRDPLVPAADAE
                     AWRLHASGPFSFRLLPGGHFFHREDKDRFFAELEDVLWDGVR"
     aSDomain        9506..10156
                     /ASF="active site serine inconclusive"
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C0216_RS07670_Thioesterase.1"
                     /evalue="1.30E-35"
                     /label="C0216_RS07670_Thioesterase.1"
                     /locus_tag="C0216_RS07670"
                     /protein_end="246"
                     /protein_start="29"
                     /score="115.8"
                     /tool="antismash"
                     /translation="RLFCFHHAGGSPWSFAGWGQHLGRGVEVVPVSMPPRSSAPGGVPG
                     AVSTMDALVREVTRQLTPFLDGPYAFYGHSMGSLVAHGAAQRIVAAGRRPPASFVAGAH
                     RAPHLQAAPGPTVGLSDSASLELLLGLGGAGRLLRESPARMRSVVHRLREDLRVAATHR
                     NPVAGHRPLPCPVHAMLGDRDPLVPAADAEAWRLHASGPFSFRLLPGGHFFHRE"
     CDS_motif       9509..9535
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C0216_RS07670_0001"
                     /evalue="4.30E-03"
                     /label="NRPS-beforete1"
                     /locus_tag="C0216_RS07670"
                     /protein_end="39"
                     /protein_start="30"
                     /score="10.2"
                     /tool="antismash"
                     /translation="LFCFHHAGG"
     CDS_motif       9701..9766
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C0216_RS07670_0002"
                     /evalue="7.00E-08"
                     /label="NRPS-te1"
                     /locus_tag="C0216_RS07670"
                     /protein_end="116"
                     /protein_start="94"
                     /score="24.9"
                     /tool="antismash"
                     /translation="DGPYAFYGHSMGSLVAHGAAQR"
     CDS_motif       10031..10048
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C0216_RS07670_0003"
                     /evalue="9.90E+01"
                     /label="NRPS-beforete1"
                     /locus_tag="C0216_RS07670"
                     /protein_end="210"
                     /protein_start="204"
                     /score="-3.1"
                     /tool="antismash"
                     /translation="VHAMLG"
ORIGIN
        1 gtgcgccagg gccagggaca gcccgcgccc gaacatcggg tcggtggtgc acgcggcgtc
       61 gccgacctgg aggagcccgc ggacgggccg ctgccggtcg gttgccgtgc cgcgcaggac
      121 gttcggcggc atgccggtcg cacggaccgg gccgagcggc tgcgcggcgt cctcctcgac
      181 ccacggcgcg aggtacgggg agagccggca ggcggcggtg aacgccccgg cgccgcgcag
      241 cgcggccaag gcggggtcgc cgggcagggc gccgagggtg atggcgaaga cgtcgttgtc
      301 ggcggggtgc aggacggccg agtagtggcc ccagatcccc ccggcggcat tgccccggtt
      361 gagcggtccg ggcagcgggc cgcaggggcg gcgcagccgg tagaagcggc cgtacgcgtg
      421 cagccggctg gggccgctca ggtcgtccgc caccggcagc ccggccgccg cgagccagga
      481 ccgggacgcg gagcggcgcc cggtcgcgtc cacgacgagg cgggccggga tccgctcccc
      541 gctgtccgcg acgactcccg ccacccggtg gcccgacggc ccggccgggg ccgtcagcag
      601 cccggcggcg gccgtgcggt ggctgaagga cacgcccggc gcggccgctg cggcccggtg
      661 caggacgacg tccaggaggg agcggcgggc gaccagggtc accagctcct cctcgccggg
      721 cccggcgggg ccgccgggcg ccgccgcggt caggtcgagg agccgtgcgc cgtgcgccag
      781 caggctgccg agcacgtcgg gggcgtgccg gcgcagcatg cgcacgccga gcgcgttcag
      841 gatgtggtcg tggacggcct gcgggacccc cggccgccgc caccgcagcg cgctctccac
      901 ggccggcccg tccggcgggg ggccgccccg ctccaggacc cgcacccggt ggccgcgccg
      961 gccgagcgcc agggcggtgg cgaggccggc ggtgccgccg ccggcgatga cgatggctgc
     1021 catcgtcagc gacgcctgcc gtggacgaag aacacgcgcc gcacccggcg caccgcgcgc
     1081 ggcggtacgg ggtcgggcca gcgcccgaag tcggcccccg gctcgccggg ctgtacggcg
     1141 cggacgtcga acccgtagcg cgcgaacagg tcttcgggct cgtcggtgcc gaacacccag
     1201 gggcaccccc agcccgcgaa gacgtcgagc agcccccgca tgtgggggag tgtcagcgcg
     1261 gcggcgttga cgatgtcggc ggcgatgcgg ctgccgggcg cggagacggc cgcgacccgc
     1321 tccagcatcc ggtgcgcgtc ggcctcgggg atgtagtaga gcagcccctc cagcagccac
     1381 gtcgagggaa cggacgggtc gtagccggcc gcggcgaggc ggtcctccca gccgtcggag
     1441 gtgaggtcga cggccaccgt gcggtggtcg acccgcggct cggcgccccc cagccgctcg
     1501 gccttgaagt cgaggacggc ctgccggtcg acctcgaagt agcggacgcg gccgggccag
     1561 gggagccggt aggcgcgcga gtccatgccg gcgggggcca gcacgatctg cgtcgtctcc
     1621 ggtgcgcggg ccgcttcctg gaggaagtcg tcgaagaacc gggtgcggat ggcgttgtag
     1681 tcgggggtgc tgggcagggt tcgcggccgg ccgggaggga aggtcgcctc gcggatctcc
     1741 cgcagcagtt cggggccggc ctcgccgacg agtccggcgg cgtacgggtc ctcgtacagc
     1801 cggtcttcgc ggcgggtctc ggcggcccgc agcgccgcgg tgagcagggc ggtccgttcg
     1861 acgggattga gtacgggcat gggggctcct tggtgcaggg gtgggccggg ggccggcggg
     1921 ccgggcggcg cggtcagccg ttcggcgcgg cggtcagacg cccccacagc gcgtcgtcct
     1981 gccggaagag gcggtccttg acctgggtgg ggcggatgcc ggaggcgacc gccgcgtgcc
     2041 agtcgcggtc gaaggcctcg gcgtcgagga ggcgcaggga cggggcggcc aggcggccgg
     2101 ccgcgcgggc ggctgcgtat ccggcggact ctgcggacag cgccccgtcc agggcggcgg
     2161 tgaagcgcgc ggccccggcc ggcggccagg gcccgcgggg ggagacggcg aagacgtagt
     2221 gcggggtgcc gccgggcgac ggctcgacgc ggcaggcggc gttccgcagg gcgagcccgc
     2281 cggggcggag ggcggtgtgc agggcgcgga cggcctcggc gtcccgcagg cgctcgccgg
     2341 cggcgtcgga gcggccgccg cggccggcgt actcgacgcg ggggacccct ccggcggtgt
     2401 ccacgacgcg gaccacgtcg ccgacggcgt accggtacag gccgccgacg tggctgaaca
     2461 ccacgtggta gtcgcggccg gcttcgagct cgtcgggcag cagggtgtcg ctgtcggggc
     2521 ggaggtcctc ctccgcgggc acgaactcgt acaccgaggc ggaggcggtg aggctgccgg
     2581 ccgacgggtg ccggtccagc gccacgccga ccggcccctc ggaggcggcg accggcgcgg
     2641 gcaggacggc gacgtccgcg ccgaactccc cgctcagcgc cggcaggtag agggaggcca
     2701 ctccggtggt ccagcagaac agcgcccgca tccggggcca cacctgcgcg gggcgcaccc
     2761 tgcccgagga atcggcgagc cgctccagtt cggcggcgcg cgccgggtcg ggacggccgt
     2821 ggggcagccc gccgagggtg ccgtcgcgga cctccttcac gatgcgcggc caccacaggt
     2881 tcagctggta cgggacggcg gcgaccatcg ccgggttgat gccgatcatg cagcgcaggt
     2941 cggcctggac ggcgagccgg agccgcaggt aggcccgctc caggtggtcg gcggggtcgg
     3001 tggggacggg cagtgccgcc cagggcgcgc cggtgccggg ttcggcggag aggggttcgc
     3061 cgaagcgggc gccgaagtcg acctggctgg ctccgacgtg tggccggccg tcggcggtgg
     3121 tgggcggggc ggtgagcggg tcgtgcttga ggttcaggac cgcgtcgggg cgctccagca
     3181 cctcggggaa gtgctcgacg aggggggccc aggccgcgta gtagaacggc atgaaggtcg
     3241 tcttcatgaa gcggggcgtg acggggacct tcttgtgcgc tccggtgctg ccgctgctgg
     3301 tgaagtacac ggcgggggtg tcggcggaca gcaggttccg ctcgccggcg gccgtccgct
     3361 cgatcagcgg cgcgtggtcg gcgtagtcgc ggacggggac ggcggagcgg aagtcctcca
     3421 tcgtcaggac gcggcccagg ccgtgggccc ggccgaacgc ggtggaggcg ttgaagccga
     3481 cgaggtcggt cagggcgcgc cgctgcgcct cggggaggtc ggtgtggtcc cgggtgagcc
     3541 gggtgcgggc ggcgaagacg cgctcgcggt agcggacggc gtggcccggc gcgtgccaga
     3601 cggaggaaac gttcatgcgg ccagtacctc cagcacggcc ctcgccgcgc ggtcggggtc
     3661 ggactgctcg gcgagcacgg tcaccggcac gtcgcggacc tcctccagca ggatcttccg
     3721 caggttcttc tggtagcgct cgaagtccgc ccagcggccg tcccggccct cgtactccag
     3781 ggcgttgagc cgggccccgt ccgccctgcg gtgccaggcc gtctcggggg acacgtcgag
     3841 gaacaccacc cgcgcgggct gcgggaagga gcgccaccag gagtacaggc ggctgtcgct
     3901 gacgcccgcc atccggcact tcgcgaggat cttgtagtag tacgagtcca cgagcacggg
     3961 gacgcgcggg tcggggccgt ccagctggtc cctgaggtgg accacggccg tctgcagcac
     4021 gctcgccatg aagtcggcgg agtacgcggt gcccaggccc ggcaggaggt gctcgtgcac
     4081 gtcctgccgc agcctgttga ccaggtcgtg ctcgggggcg acgaacgccc tgtccgccga
     4141 caccagccgc cactccggca gctgcgccgc gaggcgccgc atgaccgacg acttgccggc
     4201 cccgtcgggc cccagcagca cgtggaagcg cggtccgctc atcgggcggc ggctgtcgtc
     4261 tcggcgcgca gggcgaggcg ttcgcgcagc tccgggtcgt cgccgacggc gacggggtgt
     4321 ccgacgagct ccagcatggg caggtcggag acgtggtccc cgtacgccca gcagtccccg
     4381 gggtcggtgt cggggcggga ggccaggtgg gccagtacgg cctcgcgctt gccctcgccg
     4441 atgacgggca cggcgacctc gccggtgtac gtcccgccgt cggccagcgg gcgcgtgcac
     4501 aggatcgcgt cggcgccgat ctcgcgggcg agcggctcca ggaggggcgg gaaggagccg
     4561 gagaccagga cgacggacgc gccggccgcg cggtggcggt gcagggcctc gcgcgtggag
     4621 tcgatccaga acccgcgggt ggaggcggcg cgttcggtgt accagtcgtc cgccagcgcg
     4681 gtgaccgccg cgacgtcctc gccggcgtag tgccggtagt aggcgcggtt gatctcctcg
     4741 cggggcacgc cgtcggcggc gcgccgccgg agcacggcca tgatccgctc gtactcctcc
     4801 tcgccggcag cgccgtgcag gcgcaccagc cggtagtgga ggaagtcgaa catgctcttg
     4861 acggtgatca gcgtctcgtc gacgtcgctg aagacggtgt ggggccgggt ggtgtctgac
     4921 atgggttggc tccttgcggg ggcggtcggc ggcccgctgt ccgtacgggc cgccacttca
     4981 ggtgcagctg tccgccgtgg tcacgacgcg cccacctcgg accgcgccgg ggcggtggcc
     5041 gccgcctcgg ccgtcgccag ggccacgccg tcctggaggg agcgctgcgc cgcccgtgtc
     5101 tccttggcgt gggagacctg cgtgtcgaag gcggtgaacc ggacgtccat gcccgcggtc
     5161 tccgacccgc cctgggtcac ggacagtgcg cagtggaacg cggtccgcgt gtggtgcggc
     5221 gagcggtggg acaggacgtg gcaccgcacc accgcgggca gcgggaagag gaagttgcgg
     5281 taccgggtgg agaaggagtc gatcacgaag tacctgctgc cgacggcctc cgtcggcagg
     5341 tagaactgct cggccaccgc gatgaacatc tgccgcgcgg cctccgtggc gagcatcccc
     5401 tgcatgtgct ggccggtgac gtggtcggac atgacctcgt tgccggcgtc cacgagcagg
     5461 tcggcttcgt accagtcggt gccggccggg cgcgggtcgg agatcagcac gttctccggg
     5521 cggtgcttgt gtgcgatctc ccgtccggcg cgggtgtgca gggcctggtc gtcgatggcg
     5581 acccggtccc cgagcccgcg gcggtccagg gtgtcgcgga tgaacgccac gtcgaacagg
     5641 gagacgccct gcccgagggc gatgcggacg gggccgctgc cggcgtcgtc cgggaacgcc
     5701 ccgctcctga tgcgcttggc gagctgggag accgtgaaca cgccttcgtg gtgggcgaac
     5761 tcggcgaaca cgtcgcccac gaggaagagc gcgcccgtcc tggcctcgcc ggcctgggat
     5821 gtttcctggg tcatggctgg tgacctttcc cgttgtcctg ggtggatgtc gtggcgcgcg
     5881 gccccccggc ggtcgtcgcc cggtccgccg ggggctcatc gcgtcattcg gcggtgcgcc
     5941 ggctcagttc ggggaacttc gggtcgctgc ccagggcgcc gccctccgcc cagcggttgc
     6001 gcggcacctc ctggatgtag acggtgatct tgtccagcgg tgctcccgtg acgcgccgga
     6061 cggtccgggt gagctcctcc aggagctccc gggactgggc gtcgtcctgg ccgtcccagg
     6121 tggtgacgga gatgatgggc atgggacccc ttccgggttc tggtggttct gctctgccgc
     6181 gtcgggccgg tcaggtctgc ggagcggcgg tggtccgcag gtccgggaag gacgggccgc
     6241 gcagcttcag gtgggcgtgg atgtccccgc cgaggccgaa ctcgccgatg gcgccgccgt
     6301 agctgcgctg gtagctcatc cacagcacga ccacgttgat gatccggttg gcgcccggga
     6361 ggtccgggtc ggggaacggg ttcggcaggg tggcgtggta gctgccggag ccctcggcgt
     6421 cggtggtgaa cacgttgggc acgccgagcg ggcccgggcg ggtggggccc gcggggtcga
     6481 ggtcgcgggc ccgcagcgac atcaccgtgt agacgctgtt cggtacgagc cgggagaacc
     6541 ggaagtcgaa ggtccccgcg tggccgtcgg gggtgacggc gacctccagc gtgccgctcg
     6601 cccgcatcca ctccccgaac gtcaccggct cggtgacctt ggggccgtcc tcggcccgca
     6661 ggtcggggat cggcacctcg tggaaggcgt ggctggggcg tacgacccgg tcggcgtcgg
     6721 gcagttgctc gggcgcgaac accatcgggt agttgttgca cggcaggggc agcggcagcg
     6781 tgtgcaggac gacgtcccgg tccgcgccgt ccgggtcggt gtccagctcc cgtacgaccg
     6841 tgtacggggc gagccggccg aactccggga cggggctggc gggcgagacg accgccgcgc
     6901 cccacccggg tgcggtcccg ccgcccgggg cgggccggtt gaccatgccc accaccagga
     6961 agttgccgtc gccgtccatg acctcgctcg gcgggtacag cgggccctgc gtggtcgggg
     7021 agagctcgtg gacctccctg cggtgcgggg tggcggcccc ggccggggcg gccgccgtcg
     7081 cggtctcaga catcggcgcg ctggtagacg cgggtgtaga ggccgctctc gccgccgacg
     7141 atgtagcggt cgccggcctg ctggacgatc tcctggtact cgggcgaggt gaagaccgcc
     7201 tggtactgct cgggcgcgca ctcgaagccg gacacgaaca tcacgccggg ctcggtgctg
     7261 aggagggagt ggtacgcgag gaacgccttc acctcgtcgg cccgccggac gacgttgaag
     7321 atcgtgttct cgcgccactc gcggtacgcc gcgtactcgc ggggcttgac ctcgatgtgg
     7381 cgcagctgga cgtacggggt cgccgggacg ggctcggcga cgtccttggg ggcctcgacg
     7441 aagtcgagga gctgacggcg gaagtcggtg gccaggtcct cggagacgag gtcccacagc
     7501 tcgtgccagc gggcgttgag ggcggcgacc tcggacacct ccgcgacggg ggtgatctcc
     7561 aggaggttgt cgtcgtccag gctgcggtag agggcgcggc cggcgaccgg ctccttccgg
     7621 ttgaactgct gccactgctc caggacgtgg tccgccttgc cgggggccgc ggcgacttcg
     7681 ttcaggacga ggagggtgga ggacatgggg tgttcctttc ggacggtacg ggaagggcgg
     7741 tgggtggtgc ggcgggggac ggcgcggggt ggggcccgtt ccgggggatg cgtgccgaac
     7801 cggtgtgggg atgcgagggg gccggggatc gggaaggccc cgtggacgcg ggcccgtcgg
     7861 gcccgccgcg tggggggtgc gaggtgttcg aggggcgggc gtcaggcgac ggggacgccc
     7921 ttggaggcga tcaccgcggc ggcttccgcg acggtctgct ccgggacgag ctcgcccatc
     7981 tcgatctcca cgccgaagga cagctgcagg cgcagcgcca gctcctccag cagcagcgag
     8041 tcgatctcca tggcctcgaa cgtggtgtcg ggcgtgacgc ccgccgtcga gacctccagc
     8101 tcaccgagga cggtgacgac ccggctgacg atcgaattct ccattacatt cctccgtgaa
     8161 tcaaggggga acgccgaaat ccgagggcct gtgcgcccgt tggacacggc ataccgccgc
     8221 cgcgagaaag aatggaaccg gtgaattcag gggatcgaat cctccgcgtc ccaacgcatt
     8281 catcatggga tccgcgaacg ggcggaggaa gtgtctcccg atcatggtgg tgcgaacacc
     8341 acctgtggga aaaccggttg cgccggtgcc ccgtacggct caggccgcct cggccccgaa
     8401 cccggccggc tcgtcccggc ggccggcccg ctccgccccg gactgggcga tgtacaaggt
     8461 caggagggat tccgggctct ctggcacccg catcaccacc cggtcgaaga ccgtcacccc
     8521 ggcctgcggc aacgcgatcc tgcgcagccc ctgattgggc ccgaccacct cgtgacagtc
     8581 ccacagttcc gcgaaatgcg ggctcatctc gctgagccgg cccgcaattc ggccgaattc
     8641 cgggtcctgt gggaagcggg ccgactgcgc ccggaactgc gcgacgagac cgcgccccac
     8701 ctcctccgtg tccgggtatt tctcacgggt cgccgactcg gtgaagaact tcacgacgca
     8761 gttgtgcccg cgcgtgccga tgtggaaaag ggagcgcgcc gagcggctgg cgagttcgac
     8821 gcgcccgtac cggtcggtga tgtacgccgg attcggcgac cagccctcga cgagcgcccg
     8881 catccgcgcg tgcccgccct cctggccgcg gtcgggtgtg cgcggcggcg ggttgtggcc
     8941 ggccagccgg tggaagtagt cgcgctcgca gtcctccagc cgcagggcgt tgctgaccgc
     9001 gttcacgatg ccttcggaga cgttgatgtc ccggccctgt tcgagccagg tgtaccagga
     9061 caccccgacg ccggcgaggg ccgccacctc ctcgcgccgc aggccggggg tccgccggcg
     9121 gtcgaagttc accagcccga cctcttccgg cgtgatctgc tcccggcggg cccgcaggaa
     9181 gtgccgcagc ctcgctcgcc gcagcagtcc ggatgccggt gcggatgcag ctgtctgcat
     9241 gtctcgactt ctttccttct acacaggact tcggtgacgg atggcggtgg gcggacggcg
     9301 ggtgccgggg ggagggagcg gggcgtgcgg ctccggctgt cggtcccacc gctggtggtg
     9361 gcaccaaccg gttcgacaga cactccctcc cgtcggcacc cggcatcaca ctggcggcat
     9421 gttccacaca gcggagaaca tcccggcagc cacggcggcg ggtacggcgg agcgggttcc
     9481 gaggaggagc aggaccgctc ggctccgcct gttctgcttc caccacgcgg gcggcagccc
     9541 ctggtcgttc gcgggctggg ggcagcacct cggccgcggc gtcgaggtgg tcccggtgtc
     9601 catgcccccg aggtcgtcgg ccccgggggg cgtccccggc gccgtcagca cgatggacgc
     9661 cctcgtacgc gaggtgacac gtcagctgac tcccttcctc gacgggccct acgccttcta
     9721 cgggcacagc atgggatcgc tggtcgccca cggtgcggcg cagcggatcg tcgccgcggg
     9781 gcggcgccca cctgcgtctt tcgtggccgg tgcccaccgc gcgccgcacc ttcaggctgc
     9841 gcccggccca accgtaggac tgtccgatag tgcgtccctc gagctgttgt tgggtctcgg
     9901 tggagccggt cggctgctcc gggagagccc cgcgcgcatg aggtccgtgg tgcaccggct
     9961 gcgcgaggac ctccgcgtcg ccgccaccca ccggaatccg gttgccggcc accggccgct
    10021 gccgtgcccg gtgcacgcca tgctcggtga ccgggacccg ctggtcccgg ccgcggacgc
    10081 ggaggcgtgg cgactccatg cgagcgggcc gttctcgttc cgcttgctgc ccggcgggca
    10141 cttcttccac cgcgaggaca aggaccgctt cttcgccgag ttggaggacg tcctgtggga
    10201 cggcgtacgc tgacgctcat tcacggctgc ggtgttgtgg ccgagttccc ggtgtccggt
    10261 gggcccggcg gtgcctgcgc ctgtgtggcc cgaaccgtgc gcagtgtgat tctcgccgct
    10321 gacagtgcgt gtcggtgtga cctaccttcg gtcggtatgg acgccacgcc ccccggggcc
    10381 gagacgaaga agaagcggcg cacccagctg gcgcacttcc tgcgcacccg ccgagccgcg
    10441 ctctcccccg aggacgtggg cctggcgacc gccggcaggc gcgccagggt cggcctgcgc
    10501 cgcgaggagg tcgcggtcct ggccggagtc agcgcgtcct ggtacgcctg gctggagcag
    10561 ggccgcccca tcaaggtgtc ggacggcatc ctggacgcga tcagctccgc cctgcggctc
    10621 gacgagctcg aacgggtcca cctgtaccgc ctcgcggacg ccaacccgcc cgtccccctg
    10681 cggcccctcg gcaccgccga cccggaggtc ttccagcgga tggtcgacac ccagctgtcg
    10741 gggccctcct gtgtcatcga ccgctactgg gacgtcgtcg ccgccaaccg ccaggccgcc
    10801 gacctgctgc gcctgacccg tgacggcaac ggca
//
