LOCUS       NZ_QOIM01000069         4693 bp    DNA     linear   CON 04-MAY-2024
DEFINITION  Streptomyces reniochalinae strain LHW50302
            NODE_42_length_4693_cov_19.1299_ID_83, whole genome shotgun
            sequence.
ACCESSION   NZ_QOIM01000069
VERSION     NZ_QOIM01000069.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces reniochalinae
  ORGANISM  Streptomyces reniochalinae
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Li,L.
  TITLE     Streptomyces reniochalinae sp. nov. and Streptomyces diacarnus sp.
            nov. from marine sponges
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Li,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2018) Department of Pharmacy, Ren Ji Hospital
            School of Medicine, Shanghai Jiao Tong University, Pujian, Pudong,
            Shanghai 200127, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-FEB-2016
            Assembly Method                   :: A5-misseq v. MARCH-2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003323735.1-RS_2024_05_04
            Annotation Date                   :: 05/04/2024 01:54:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,751
            CDSs (total)                      :: 6,669
            Genes (coding)                    :: 6,475
            CDSs (with protein)               :: 6,475
            Genes (RNA)                       :: 82
            rRNAs                             :: 6, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 4, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 66 of 194
            Pseudo Genes (incomplete)         :: 154 of 194
            Pseudo Genes (internal stop)      :: 16 of 194
            Pseudo Genes (multiple problems)  :: 41 of 194
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:03:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 4693
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            QOIM01000069.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            complement(<1..4649)
                     /locus_tag="DQ392_RS35920"
                     /old_locus_tag="DQ392_33915"
     CDS             complement(<1..4649)
                     /NRPS_PKS="Domain: PKS_DH (162-325). E-value: 4.3e-58.
                     Score: 188.3. Matches aSDomain:
                     nrpspksdomains_DQ392_RS35920_PKS_DH.1"
                     /NRPS_PKS="Domain: PKS_KR (625-803). E-value: 6.3e-53.
                     Score: 171.4. Matches aSDomain:
                     nrpspksdomains_DQ392_RS35920_PKS_KR.1"
                     /NRPS_PKS="Domain: ACP (901-973). E-value: 3.7e-29. Score:
                     92.9. Matches aSDomain: nrpspksdomains_DQ392_RS35920_ACP.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (995-1410). E-value:
                     1.7e-180. Score: 592.4. Matches aSDomain:
                     nrpspksdomains_DQ392_RS35920_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /locus_tag="DQ392_RS35920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DQ392_33915"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_220182696.1"
                     /sec_met_domain="PKS_AT (E-value: 9.7e-26, bitscore: 76.9,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 5.6e-58, bitscore:
                     181.9, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.3e-16, bitscore:
                     47.2, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="mod_KS (E-value: 1.9e-225, bitscore:
                     734.6, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 9.4e-95, bitscore:
                     303.0, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDPMLEEFAAVAATLSYHEPVLPIVSTVTGQLVVEGQLTDPGYWV
                     DQVRRAVRFADGVTATDASVFVEIGPDGVLSALAQHSVDDATVAVPAVRKDGDEAQAFV
                     EALGRLHTTGVPVDWTAYLGHAPVHVDLPTYAFQHQRYWIEPNRAAGDATHAGLTPVDH
                     PLLTAATELPGTETLLFTGRISLATHPWLADHEVLGTVLVPGTGLLELALQAGHEAGLP
                     EVRELTLREPMLVPATGALEIRVVVGAPDDAGERSVSVHSRGEADTTAAWMLHAEGTLT
                     AAAATPPATEQVWPPVGAVSVDVSGVYEGLLGRGYVYGPVFQGLRAVWERGDEVFAEVE
                     LPEDGVVDAGRFGLHPAVFDAAMHALLVVGGGDAETVLPFVWSGVRLHAAGASRLRVRI
                     SRVSGAMALADAAGAPVLTVESLVSRPVSVEQLGARNEALFRVEWSPLSVGEVAGAGTE
                     MVSVGDPVPGLSVDGFDDLVSLAESGSVAPLVVSGCCGEEESGDVVGAVRRSVGGVLDL
                     VQGFLGDARLAGSRLVVVTRGAVAVGSGDDVVPTAAPVWGLVRAAQAEHPGRIVLVDVD
                     GASASVGVVAGALASGEPEVAVRSGRVLVPRLVRVAAAAHEVPVVDPASTVLITGGTGG
                     LGALVARHLVTERGVRHLLLVSRSGSDAPGAAELSAELVAAGALVDVRACDVTDRGALS
                     EVIGSVSVEHPLVGVVHAAGVGDTGLIESVSVERLAGVFGPKVDAAWFLHELTCELDLA
                     FFVLFSSAGGLVLPAGQASYAAANVFLDGLAAYRRGLGLVATSLAFGLWETSTGLTRWL
                     GDADVERMRRQGLPALSVEAGLAGFDAGLASSEALLVPLRVDVPALRGRADVPALLRGL
                     APPRARRAAQVSTASDLVERLAGDDREQVLLDLVREKAAAVLRYPSSESVEPERAFQDL
                     GFDSLTAIELRNELNAATGLRLSATLVFDHPNAQAVARRIESELVGDAAPSSGSVVATS
                     SASDEPIAIVGMACRYPGGISTPEELWQLVTTATDAIDDFPTDRGWDLDRLYDPEPGVP
                     GKSYTRTGGFLYEAGDFDPGFFRISPREALGMDPQQRLLLETSWEAFERAGVDPRSRRG
                     SRTGVFAGLMYHDYGLGTPGATSTGSLLSGRVSYTFGFEGPAVTVDTACSSSLVALHLA
                     VQALRSGECDLALAGGATVMSTPGTFVEFSRQRGLSPDGRCKAFGAGADGTGWGEGVGM
                     LLVERLSDARRNGHPVLAVVRGSAVNQDGASNGLTAPNGPSQQRVIGQALANAGLSAFD
                     VDAVEAHGTGTALGDPIEAQALIAAYGQDRPAGRPLWLGSIKSNIGHAQAAAGVAGVIK
                     MVMAMRHGVLPQTLHAEEPSPHVDWSAGTVELLTEARQWTTSDRPRRAGISSFGIAGTN
                     AHVVVEQVDEPAAVEAVAAPPVVPWLVSAATAEGVSAQASKLLTHLDTEADAAAVGAAL
                     VSRTVLEHRAVVVGADRAELIEGLRALAAGEVCAGVVAGSGSGGKLGLLFAGQGSQRLG
                     MGRSLVAAFPVFAEALDEVVGVLDPLVSFS"
     source          1..4693
                     /collected_by="Lei Li"
                     /collection_date="2015-04-30"
                     /country="China: Xisha"
                     /culture_collection="CCTCC:AA 2018013"
                     /culture_collection="DSM:106194"
                     /db_xref="taxon:2250578"
                     /identified_by="Lei Li"
                     /isolation_source="marine sponge"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces reniochalinae"
                     /strain="LHW50302"
                     /submitter_seqid="NODE_42_length_4693_cov_19.1299_ID_83"
                     /type_material="type strain of Streptomyces reniochalinae"
     region          1..4693
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..4693
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..4693
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[<0:4649](-)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..4649)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     CDS_motif       complement(75..122)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DQ392_RS35920_0008"
                     /evalue="1.80E-04"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="DQ392_RS35920"
                     /protein_end="1525"
                     /protein_start="1509"
                     /score="14.1"
                     /tool="antismash"
                     /translation="LFAGQGSQRLGMGRSL"
     aSDomain        complement(420..1664)
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DQ392_RS35920_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="1.70E-180"
                     /label="DQ392_RS35920_PKS_KS.1"
                     /locus_tag="DQ392_RS35920"
                     /protein_end="1410"
                     /protein_start="995"
                     /score="592.4"
                     /tool="antismash"
                     /translation="IAIVGMACRYPGGISTPEELWQLVTTATDAIDDFPTDRGWDLDRL
                     YDPEPGVPGKSYTRTGGFLYEAGDFDPGFFRISPREALGMDPQQRLLLETSWEAFERAG
                     VDPRSRRGSRTGVFAGLMYHDYGLGTPGATSTGSLLSGRVSYTFGFEGPAVTVDTACSS
                     SLVALHLAVQALRSGECDLALAGGATVMSTPGTFVEFSRQRGLSPDGRCKAFGAGADGT
                     GWGEGVGMLLVERLSDARRNGHPVLAVVRGSAVNQDGASNGLTAPNGPSQQRVIGQALA
                     NAGLSAFDVDAVEAHGTGTALGDPIEAQALIAAYGQDRPAGRPLWLGSIKSNIGHAQAA
                     AGVAGVIKMVMAMRHGVLPQTLHAEEPSPHVDWSAGTVELLTEARQWTTSDRPRRAGIS
                     SFGIAGTNAHVVVEQV"
     CDS_motif       complement(639..683)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DQ392_RS35920_0007"
                     /evalue="1.60E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="DQ392_RS35920"
                     /protein_end="1337"
                     /protein_start="1322"
                     /score="11.1"
                     /tool="antismash"
                     /translation="GSIKSNIGHAQAAAG"
     CDS_motif       complement(744..788)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DQ392_RS35920_0006"
                     /evalue="4.00E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="DQ392_RS35920"
                     /protein_end="1302"
                     /protein_start="1287"
                     /score="12.6"
                     /tool="antismash"
                     /translation="VEAHGTGTALGDPIE"
     CDS_motif       complement(1164..1211)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DQ392_RS35920_0005"
                     /evalue="7.50E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="DQ392_RS35920"
                     /protein_end="1162"
                     /protein_start="1146"
                     /score="21.0"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVA"
     aSDomain        complement(1731..1946)
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DQ392_RS35920_ACP.1"
                     /evalue="3.70E-29"
                     /label="DQ392_RS35920_ACP.1"
                     /locus_tag="DQ392_RS35920"
                     /protein_end="973"
                     /protein_start="901"
                     /score="92.9"
                     /tool="antismash"
                     /translation="LDLVREKAAAVLRYPSSESVEPERAFQDLGFDSLTAIELRNELNA
                     ATGLRLSATLVFDHPNAQAVARRIESE"
     aSModule        complement(1731..4163)
                     /domains="nrpspksdomains_DQ392_RS35920_PKS_DH.1"
                     /domains="nrpspksdomains_DQ392_RS35920_PKS_KR.1"
                     /domains="nrpspksdomains_DQ392_RS35920_ACP.1"
                     /incomplete
                     /locus_tags="DQ392_RS35920"
                     /tool="antismash"
                     /type="pks"
     aSDomain        complement(2241..2774)
                     /ASF="KR domain putatively catalyzing L-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N found: True"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DQ392_RS35920_PKS_KR.1"
                     /evalue="6.30E-53"
                     /label="DQ392_RS35920_PKS_KR.1"
                     /locus_tag="DQ392_RS35920"
                     /protein_end="803"
                     /protein_start="625"
                     /score="171.4"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: (unknown)"
                     /tool="antismash"
                     /translation="TVLITGGTGGLGALVARHLVTERGVRHLLLVSRSGSDAPGAAELS
                     AELVAAGALVDVRACDVTDRGALSEVIGSVSVEHPLVGVVHAAGVGDTGLIESVSVERL
                     AGVFGPKVDAAWFLHELTCELDLAFFVLFSSAGGLVLPAGQASYAAANVFLDGLAAYRR
                     GLGLVATSLAFGLWE"
     CDS_motif       complement(2286..2396)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DQ392_RS35920_0004"
                     /evalue="1.00E-15"
                     /label="PKSI-KR_m4"
                     /locus_tag="DQ392_RS35920"
                     /protein_end="788"
                     /protein_start="751"
                     /score="50.2"
                     /tool="antismash"
                     /translation="LAFFVLFSSAGGLVLPAGQASYAAANVFLDGLAAYRR"
     CDS_motif       complement(2673..2774)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DQ392_RS35920_0003"
                     /evalue="2.80E-11"
                     /label="PKSI-KR_m1"
                     /locus_tag="DQ392_RS35920"
                     /protein_end="659"
                     /protein_start="625"
                     /score="36.0"
                     /tool="antismash"
                     /translation="TVLITGGTGGLGALVARHLVTERGVRHLLLVSRS"
     aSDomain        complement(3675..4163)
                     /ASF="catalytic triad H,G,P inconclusive"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_DQ392_RS35920_PKS_DH.1"
                     /evalue="4.30E-58"
                     /label="DQ392_RS35920_PKS_DH.1"
                     /locus_tag="DQ392_RS35920"
                     /protein_end="325"
                     /protein_start="162"
                     /score="188.3"
                     /tool="antismash"
                     /translation="HPLLTAATELPGTETLLFTGRISLATHPWLADHEVLGTVLVPGTG
                     LLELALQAGHEAGLPEVRELTLREPMLVPATGALEIRVVVGAPDDAGERSVSVHSRGEA
                     DTTAAWMLHAEGTLTAAAATPPATEQVWPPVGAVSVDVSGVYEGLLGRGYVYGPVFQGL
                     "
     CDS_motif       complement(4002..4079)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DQ392_RS35920_0002"
                     /evalue="1.00E-09"
                     /label="PKSI-DH_m2"
                     /locus_tag="DQ392_RS35920"
                     /protein_end="216"
                     /protein_start="190"
                     /score="30.8"
                     /tool="antismash"
                     /translation="WLADHEVLGTVLVPGTGLLELALQAG"
     CDS_motif       complement(4488..4523)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_DQ392_RS35920_0001"
                     /evalue="2.30E-03"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="DQ392_RS35920"
                     /protein_end="54"
                     /protein_start="42"
                     /score="11.5"
                     /tool="antismash"
                     /translation="YWVDQVRRAVRF"
ORIGIN
        1 gagaacgaca ccaacggatc caaaaccccc acaacctcat ccaacgcctc agcaaacacc
       61 gggaacgccg ccacaagcga ccgccccata cccaaccgct gactcccctg accagcgaac
      121 agcaacccca gcttcccacc cgaccccgaa ccagccacca caccagcaca gacctcaccc
      181 gccgccagcg cacgcaggcc ctcgatcagc tcagcgcggt ccgcaccgac caccaccgca
      241 cggtgttcca acacggttcg tgacaccaac gccgcgccca cagcggcggc gtccgcttcg
      301 gtgtccaggt gcgtcagcag cttcgaagcc tgcgcggaca cgccctccgc ggtcgcggca
      361 ctgaccaacc acggcaccac cggcggagcc gccacagcct cgaccgccgc gggctcgtcc
      421 acctgctcga cgaccacgtg cgcgttggta ccggcgatgc cgaacgagga gatgccggcc
      481 ctgcgcggcc ggtcgctggt ggtccactgg cgtgcctcgg tcagcagctc gacggtgccg
      541 gccgaccagt ccacgtgggg gctgggctct tcggcgtgca gagtctgcgg cagcacaccg
      601 tgccgcatgg ccatcaccat cttgatcaca ccggccacac cggcggcggc ctgggcgtgg
      661 ccgatgttcg acttgatgga ccccagccac agcggccggc cggcgggccg gtcctggccg
      721 taggcggcga tgagggcctg cgcctcgatc gggtcaccca gcgccgtacc cgtgccgtgc
      781 gcctccaccg cgtccacgtc gaaagccgac agaccggcat tcgccagggc ctgcccgatc
      841 acccgctgct gggaagggcc gttgggcgcc gtcaggccgt tgctggcacc gtcctgattg
      901 accgcagaac cccgcaccac cgcgagcacg ggatgcccgt tccgccgcgc gtccgagagc
      961 cgctccacca agagcatgcc cacgccctca ccccagccgg taccgtccgc accggcaccg
     1021 aacgccttgc accgcccgtc gggtgacaga ccccgctgac gggagaactc cacgaacgtg
     1081 ccgggggtcg acatcacggt ggcgccgccg gccagcgcga gatcacactc accgctacgc
     1141 aatgcctgta ccgcgagatg cagggcaacc agcgacgacg agcacgccgt gtccaccgtc
     1201 accgcggggc cctcgaaccc gaacgtgtag gaaacccgcc cagaaagcag acttcccgta
     1261 ctcgtcgccc caggtgtccc caggccgtag tcgtggtaca tcagcccggc gaacacaccg
     1321 gtccggctgc cacgacgcga acgcgggtcg acaccagcac gctcgaacgc ctcccacgac
     1381 gtctccaaca acagacgctg ctgcgggtcc atccccagag cctcacgggg agagattctg
     1441 aagaacccgg ggtcgaaatc gccggcctca tacaggaagc caccggtacg ggtatagctc
     1501 ttacccggca ctcccggctc cgggtcgtac agccggtcaa gatcccaacc ccgatcggtc
     1561 gggaaatcgt ctatggcgtc cgtggcagtg gtgaccagct gccagagctc ttccggagtg
     1621 ctgatgccac ccgggtagcg gcaggccatg cccacgatgg cgatcggctc atcgctcgcg
     1681 ctggaggtcg ccacgacact gcccgaagag ggagccgcgt caccgaccag ctcggactcg
     1741 atgcgccgag ccacagcctg ggcattcggg tggtcgaaca ccagcgtcgc cgagagccgc
     1801 aggccggtcg ccgcgttcag ctcgttacgc agctcgatcg ccgtcagcga gtcgaagccc
     1861 aggtcctgga acgccctctc cggctcgacc gactccgatg acgggtagcg gaggacggcc
     1921 gcggccttct cccgcaccag gtccagcagc acctgctcac ggtcgtcacc ggccaggcgc
     1981 tctacgaggt ccgacgcggt cgagacctgg gctgcccgcc gggcccgtgg cggtgccagg
     2041 ccccgcagaa gcgcaggcac atccgcccgg ccacgcaacg ccggaacatc cacccgcaac
     2101 ggcaccaaca gggcctcgga cgaagccagc ccggcatcga acccggcaag ccccgcctcc
     2161 accgacaacg ccggcaaacc ctgacgacgc atccgctcca catcagcgtc acccaaccac
     2221 cgcgtcaacc ccgtcgacgt ctcccacaac ccgaacgcca acgacgtcgc aaccaacccg
     2281 agcccacgac gataagccgc caacccatcc aagaacacat tcgccgccgc atacgacgcc
     2341 tgccccgccg gcaacaccaa accacccgcc gacgagaaca acacaaagaa cgccaaatcc
     2401 aactcacacg tcagctcatg caagaaccac gccgcatcaa ccttcggccc gaacacacca
     2461 gccagacgct ccaccgacac cgactcgatc aacccggtat ccccgacccc ggcagcatgc
     2521 accacaccca ccaacggatg ctccaccgaa acagacccaa tcacctccga aagcgcgccc
     2581 cgatccgtga catcacacgc cctcacatcc accaacgcac ccgccgcaac caactcagcc
     2641 gacaactccg ccgcgcccgg cgcatcggaa ccactacgac tcaccaacag cagatggcgc
     2701 accccgcgct cagtcacaag atgacgagcc accaacgcac ccaaaccgcc ggtaccaccg
     2761 gtgatcaaca cggtgctcgc cggatcaacc acgggcacct catgcgccgc agcggccacc
     2821 cgcaccagac gaggcaccag cacccgaccc gaccgcaccg ccacctccgg ctcaccggac
     2881 gccaacgcac ccgcaaccac acccaccgac gccgacgccc catccacatc caccagcaca
     2941 attcgccccg gatgctccgc ctgcgccgca cgcaccaaac cccacaccgg cgccgcagtc
     3001 ggcaccacgt cgtcacccga ccccaccgcg accgcgcccc gcgtcaccac caccaagcga
     3061 gaccccgcaa gccgcgcatc ccccaagaac ccctgcacca gatccaacac accccccacc
     3121 gaccgccgca ccgcacccac cacgtcaccc gattcctcct caccacagca ccccgacacc
     3181 accaagggag ccacagaccc cgactcagcc aacgacacca gatcatcgaa cccatccacg
     3241 gacaacccag gaaccgggtc accaaccgaa accatctcgg tcccggctcc cgccacctcg
     3301 cccaccgaca acggcgacca ctcgacccgg aacaacgcct cgttccgggc acccaactgc
     3361 tccaccgaca ccggccgcga caccaacgac tccaccgtca acaccggcgc acccgcagca
     3421 tccgccaacg ccatcgcacc actcacccgc gaaatccgca cccgcaaccg cgaagcaccc
     3481 gcggcatgca accgcacacc ggaccacacg aacggcaaca ccgtctccgc gtccccacca
     3541 cccacgacca acaacgcatg catcgccgcg tcgaacacag caggatgcaa accaaaccga
     3601 cccgcatcaa caaccccgtc ctcaggcaac tccacctcag cgaaaacctc atccccccgc
     3661 tcccacaccg cacgcaaccc ctgaaacacc gggccataca catagccccg acccaacaaa
     3721 ccctcataca cacccgacac atcaacagac acagcaccaa ccggcggcca cacctgttcc
     3781 gtcgcggggg gagtcgctgc cgccgctgtc agggttcctt cggcgtgcaa catccacgca
     3841 gccgtggtgt cggcctctcc tcggctgtgc acgctgacgc tgcgctcgcc cgcgtcgtcc
     3901 ggggctccga ccacgacgcg gatctccagt gcgcccgtcg cggggaccag catcggctcc
     3961 cggagcgtca gttcccgcac ctcggggagt cccgcttcgt ggccggcctg caacgccagc
     4021 tccagcagac ccgtcccggg taccagcacg gtgcccagca cctcgtggtc ggccagccac
     4081 ggatgcgtgg ccagagagat ccgcccggtg aacaggaggg tctcggtgcc gggaagttcg
     4141 gtggcggccg tcaggagggg gtggtcgacg ggtgtgagcc cggcgtgggt ggcgtcgccg
     4201 gcggctcggt tgggttcgat ccagtaccgc tggtgctgga aggcgtaggt cggcagatcg
     4261 acatggacgg gggcgtgtcc gaggtaggcg gtccagtcga cgggcacacc tgtggtgtgc
     4321 agccggccca gtgcctccac gaacgcctgg gcctcgtccc cgtccttgcg gacggccggc
     4381 acggccaccg tggcgtcgtc cacgctgtgc tgcgccagcg cgctgagtac accgtcgggc
     4441 ccgatctcga cgaagaccga cgcgtccgtg gcagtgacgc catcggcgaa ccgcacggcc
     4501 cgacgcacct gatcgaccca gtaacccggg tcggtgagct gcccctcgac aaccaactgc
     4561 cccgtgacag tagagacgat aggcaacaca ggctcgtgat aactgagcgt cgcggccaca
     4621 gccgcgaact cctccaacat cggatccatc aacggcgagt ggaacgcatg gctgacccgc
     4681 aaccgccgag ccc
//
